Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0001142

Physical Location & Seq

Genomic Coordinates Forward (+)

25286022 .. 25288385

Gene Structure 2364 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1185400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2364 bp

ATGGAGAACGGTCACGATTGGAAAGTCGCGGAGAAATTCTCAGGGTTGACGGTTTCCGCCGCCGACCCAATTCCTTCTTCTACCCCCACCGCCACCCCAAACAATCAAGACAGCAGCTTGATTCAGGTTATGAACGCCATGGAAGCTGCGGAGGCCACAATCAAGCAGCAGGTGGAAGAGAACATCCGATTGAGGAATCAGCTCGAACACAAGATTCTCGAGTTGCACAGGTACAAGCTCCAAGAATCCATGGCTGCCCAGTTGCCGCCTTCTGCCCGCGGATCTCAGGACCAAGCTCATCAGGTAGTTGACCCCGGCGTCGATAATCGAATCGAGAGGATTGCGAATCACGGTTCTGCACAGCATAGTCGCGGGGAATCGGAAACAAGAGAGACCCAATTAGGCGGCGGCGAAATCAATGGAGGGATGAAGGTGGTTCGGGTTTCGGGTACTCCGCGGGCGGCTGATAACGCGAACAGCGGGATATCGAGATTGTCGTCGCCGTCCACCACGACGTCGTTGTCGCCAGGTAGGTCGAGCACGGAGGGGGATTACGGAAATGGGTTGATGCCATTGACCGAATCGAACAACCCTAATAGCAGCATGTGGAAGAAGGATCTGGTTGCTAGGGTTCATGAGCACGAGCAAGAAATTATGCAATTGCGAAAGCATTTGGGGGATTATTCGATCAAAGAGGCGGAAATTCGGAACGAAAAGTATGTGCTGGAGAAGCGCATTGCTTACATGCGCTTGGCGTTTGATCAGCAGCAGCAGGACCTTGTGGATGCTGCATCAAAGTCTCTATCTTACAGGCAAGACATAATTGAGGAGAACATTCGGCTCGCTTACGCTGTGCAGGATGCGCAGCAGGAGAGATCGACGTTTGTTTCGTCTTTGCTGCCGATTCTGGCTGAGTACTCGTTGCAGCCACCCGTCCCCGATGCGCAATCAATTGTTGGTAATGTGAAGGTTTTGTTTAGGCATTTGCAGGAGAAGTTGCTTCTTACTGAGTCCAAATTGAAGGAGTTGCAGTATCAGTTAACGCCTTGGCGATCAGATTCGAATCAGCAGCCTAATAATGTTAATGTTTCTGCGCCGCAGTTATCACCACCGCCACCACCTCAGTCCGTTGCTGGAGCAGCATTGCTGAATGTAAGCAGAAATGCTCTTGAAATGGTTGCTCAGAATCAGCAAGCATATTCTTACATTGGTGGAAGCAGGGCGCCTGCTACCTCTGATGCTCAGACAACCCCGGAATGGAATCCTCATCAGCAGAGTGGTTTTGGTGGGGGTGTCGCCAACAATGTGGAACACAATGACCTGGCCGTGGGGCATAGGTATTCCCCTGTTCCAAACCGGACTATTGCTGCCCAAGATGTTCCCATTAATCATGCTGTTGTTCCAGGTGATACGCAAGGAAATCGGCATAATGAAGTAACAAGCAATAGACAGGTGACGTTTCGTGATCCTATCAGGAACACACACAGGGAGATGGATGATCAAGATGCAGAGGGAAACCACAATGAGAGAGATTCATCCAATTGGAGTGCAGGAACATCTCCTTATGCATCGCCACACAACGAATCCAGCTCCGCGTATCCCCATTTCCTACCGCCAGTTATGGAAGAGGGTTCTTCAACGTATTCAGAGGCTGCAGATGATGACCCGTTACCAGCTATAGAGGGCCTTCAAATATCGGGTGAAGCTTTTCCAGGACACGAGCTTCAAGCATGCGGATACTCCATCAACGGAACAACCAGCTGCAACTTTGAGTGGGTAAGACATCTCCAAGACGGATCGGTTAACTACATTGACGGAGCAAAGCAACCGAACTACCTTGTAACTGCTGATGATGTGGATTCATACCTTGCTATTGAAGTTCAGCCTCTGGACAACAGGAAGCGAAAGGGGGATCTCGTAAAGGTGTACGCCAACGACCACAAGAAGATAACTTGCGACCCCGAAGTGCAATCTCAGATAGAGAAGATTCTTCACAAGGGCCATGCCTCATTCAAGATATCCCAGTTCGCCGGGTACATCGACATATGGGAGCCTGCAACACTGGCCATAAAGAGGGGAGCTTACAGCATAAAGGGCAGTGGAGGACCAAGTGGAAGCGGAGTTGTAGTTGCAGAGAAGTTTTCTCCGAACACATTTGTCACAATCCCATATGGAAGTACAAACGAATTCGTAATCCATAGCGGCTCCGATAACGGAGGTGAGTATGCGTTGAAAACGGACAATGAGAGCTGTTCTAGAGATACAGTTGTGCTTACATTGAGATTGTTTATTCATAGGGCTGATGAGAGAAAGACTACAAAGAAAGGGAAAATAGGGACTTACTTTTTCAACAAGTATTTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

No domains found.

Protein Analysis

788

Amino Acids

86.59

Weight (kDa)

5.65

Isoelectric Point (pI)

50.33

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
IG_AIR9	PF23197	561 - 642	8.3e-07	AIR9 A9 domain
DUF7046	PF23080	675 - 775	1.8e-26	Domain of unknown function (DUF7046)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001142)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G08440 AT5G08440 AT5G08440 AT5G23490 AT5G23490 AT5G23490 AT5G23490 AT5G23510 AT5G23510 AT5G23510
fragaria_vesca	FvH4_1g25960 FvH4_1g25960 FvH4_1g25960 FvH4_1g25960 FvH4_1g25960 FvH4_1g25960 FvH4_3g21410 FvH4_7g23540
malus_domestica	MD03G1185400.v1.1 MD04G1186900.v1.1 MD11G1230900.v1.1
prunus_persica	Prupe.4G196300_v2.0.a1 Prupe.4G288600_v2.0.a1 Prupe.4G288600_v2.0.a1 Prupe.4G288600_v2.0.a1 Prupe.4G288600_v2.0.a1 Prupe.4G288600_v2.0.a1 Prupe.4G288600_v2.0.a1 Prupe.4G288600_v2.0.a1 Prupe.4G288600_v2.0.a1 Prupe.4G288600_v2.0.a1
pyrus_communis	pycom03g16520 pycom03g16530
rosa_chinensis	RchiOBHm_Chr1g0368691 RchiOBHm_Chr3g0485101 RchiOBHm_Chr5g0036791
rosa_laevigata	RLG00000023166 RLG00000027197 RLG00000033721
rosa_multiflora	Rmu_co8159396.1_g000001 Rmu_sc0000399.1_g000002 Rmu_sc0006040.1_g000006 Rmu_sc0006040.1_g000007
rosa_roxburghii	Rroxscaffold_1G00044160 Rroxscaffold_4G00288350 Rroxscaffold_6G00397190
rosa_rugosa	Rorug01G0343700 Rorug03G0216200 Rorug03G0216300
rosa_samantha	Rh3AG266300 Rh3BG301700 Rh3CG300000 Rh3DG295800 Rh5AG250300 Rh5BG252000 Rh5CG284000 Rh5DG260300
rosa_wichuraiana	Rw5G023020

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	160
AasI	GACNNNNNNGTC	2 cut(s)	317, 520
AatII	GACGTC	1 cut(s)	518
Acc16I	TGCGCA	2 cut(s)	864, 945
Acc36I	ACCTGC	1 cut(s)	160
AccB1I	GGYRCC	1 cut(s)	1222
AccII	CGCG	6 cut(s)	29, 279, 372, 457, 473, 1595
AclWI	GGATC	5 cut(s)	289, 624, 1460, 1804, 1920
AcoI	YGGCCR	2 cut(s)	1323, 2064
AcsI	RAATTY	3 cut(s)	35, 702, 2186
AcyI	GRCGYC	3 cut(s)	318, 515, 1223
AfaI	GTAC	6 cut(s)	233, 451, 917, 1928, 2036, 2179
AfiI	CCNNNNNNNGG	3 cut(s)	1257, 1327, 2073
AgsI	TTSAA	9 cut(s)	1021, 1172, 1638, 1691, 1727, 1877, 2014, 2233, 2350
AjnI	CCWGG	4 cut(s)	526, 1320, 1402, 1711
AjuI	GAANNNNNNNTTGG	2 cut(s)	61, 93
Alw21I	GWGCWC	2 cut(s)	542, 642
Alw26I	GTCTC	2 cut(s)	386, 804
AlwI	GGATC	5 cut(s)	289, 624, 1460, 1804, 1920
AlwNI	CAGNNNCTG	2 cut(s)	1652, 1888
Ama87I	CYCGRG	1 cut(s)	218
AoxI	GGCC	5 cut(s)	153, 1323, 1684, 1999, 2064
ApoI	RAATTY	3 cut(s)	35, 702, 2186
ArsI	GACNNNNNNTTYG	2 cut(s)	871, 903
AseI	ATTAAT	1 cut(s)	1386
Asp700I	GAANNNNTTC	1 cut(s)	1707
AspLEI	GCGC	6 cut(s)	735, 750, 865, 946, 1096, 1225
AspS9I	GGNCC	5 cut(s)	289, 775, 1684, 1999, 2105
AsuC2I	CCSGG	3 cut(s)	315, 1253, 2032
AsuHPI	GGTGA	5 cut(s)	1098, 1418, 1465, 1712, 2231
AsuII	TTCGAA	1 cut(s)	1061
AvaI	CYCGRG	1 cut(s)	218
AvaII	GGWCC	3 cut(s)	289, 775, 2105
BaeI	ACNNNNGTAYC	2 cut(s)	2253, 2286
BalI	TGGCCA	1 cut(s)	2066
BanI	GGYRCC	1 cut(s)	1222
BauI	CACGAG	2 cut(s)	641, 1718
Bbv12I	GWGCWC	2 cut(s)	542, 642
BccI	CCATC	2 cut(s)	1486, 1751
BceAI	ACGGC	2 cut(s)	487, 1310
BcgI	CGANNNNNNTGC	4 cut(s)	323, 357, 1403, 1437
BciT130I	CCWGG	4 cut(s)	528, 1322, 1404, 1713
BciVI	GTATCC	2 cut(s)	1608, 1730
BclI	TGATCA	2 cut(s)	760, 1498
BcnI	CCSGG	3 cut(s)	315, 1253, 2032
BcoDI	GTCTC	2 cut(s)	386, 804
BfaI	CTAG	2 cut(s)	627, 2256
BfmI	CTRYAG	2 cut(s)	1653, 1677
BfoI	RGCGCY	1 cut(s)	1226
BfuAI	ACCTGC	1 cut(s)	160
BfuI	GTATCC	2 cut(s)	1608, 1730
BmcAI	AGTACT	1 cut(s)	917
Bme1390I	CCNGG	7 cut(s)	315, 528, 1253, 1322, 1404, 1713, 2032
Bme18I	GGWCC	3 cut(s)	289, 775, 2105
BmeT110I	CYCGRG	1 cut(s)	218
BmgT120I	GGNCC	5 cut(s)	289, 775, 1684, 1999, 2105
BmiI	GGNNCC	3 cut(s)	1224, 2052, 2206
BmrFI	CCNGG	7 cut(s)	315, 528, 1253, 1322, 1404, 1713, 2032
BmrI	ACTGGG	2 cut(s)	253, 2017
BmsI	GCATC	8 cut(s)	558, 775, 800, 850, 931, 1228, 1495, 1577
BmuI	ACTGGG	2 cut(s)	253, 2017
BplI	GAGNNNNNCTC	2 cut(s)	23, 55
BpmI	CTGGAG	2 cut(s)	746, 1155
Bpu14I	TTCGAA	1 cut(s)	1061
BpuMI	CCSGG	3 cut(s)	315, 1253, 2032
BsaBI	GATNNNNATC	1 cut(s)	1062
BsaHI	GRCGYC	3 cut(s)	318, 515, 1223
BsaI	GGTCTC	1 cut(s)	386
BsaJI	CCNNGG	8 cut(s)	138, 249, 277, 313, 455, 1046, 1251, 1326
BsaWI	WCCGGW	1 cut(s)	1356
BsaXI	ACNNNNNCTCC	4 cut(s)	1016, 1046, 2112, 2142
Bsc4I	CCNNNNNNNGG	3 cut(s)	1257, 1327, 2073
Bse1I	ACTGG	4 cut(s)	259, 1616, 2023, 2067
Bse3DI	GCAATG	2 cut(s)	735, 1142
Bse8I	GATNNNNATC	1 cut(s)	1062
BseBI	CCWGG	4 cut(s)	528, 1322, 1404, 1713
BseDI	CCNNGG	8 cut(s)	138, 249, 277, 313, 455, 1046, 1251, 1326
BseGI	GGATG	6 cut(s)	183, 432, 790, 865, 1501, 1535
BseJI	GATNNNNATC	1 cut(s)	1062
BseLI	CCNNNNNNNGG	3 cut(s)	1257, 1327, 2073
BseMI	GCAATG	2 cut(s)	735, 1142
BseMII	CTCAG	8 cut(s)	54, 299, 903, 999, 1136, 1196, 1256, 1988
BseNI	ACTGG	4 cut(s)	259, 1616, 2023, 2067
BseRI	GAGGAG	1 cut(s)	842
BsgI	GTGCAG	3 cut(s)	342, 875, 1569
Bsh1236I	CGCG	6 cut(s)	29, 279, 372, 457, 473, 1595
BshFI	GGCC	5 cut(s)	155, 1325, 1686, 2001, 2066
BshNI	GGYRCC	1 cut(s)	1222
BsiHKAI	GWGCWC	2 cut(s)	542, 642
BsiHKCI	CYCGRG	1 cut(s)	218
BsiSI	CCGG	4 cut(s)	315, 1253, 1357, 2031
BslFI	GGGAC	2 cut(s)	920, 2350
BslI	CCNNNNNNNGG	3 cut(s)	1257, 1327, 2073
BsmAI	GTCTC	2 cut(s)	386, 804
BsmFI	GGGAC	2 cut(s)	920, 2350
BsnI	GGCC	5 cut(s)	155, 1325, 1686, 2001, 2066
Bso31I	GGTCTC	1 cut(s)	386
BsoBI	CYCGRG	1 cut(s)	218
Bsp119I	TTCGAA	1 cut(s)	1061
Bsp1286I	GDGCHC	2 cut(s)	542, 642
Bsp19I	CCATGG	2 cut(s)	138, 249
BspANI	GGCC	5 cut(s)	155, 1325, 1686, 2001, 2066
BspCNI	CTCAG	8 cut(s)	53, 298, 904, 1000, 1135, 1195, 1255, 1987
BspFNI	CGCG	6 cut(s)	29, 279, 372, 457, 473, 1595
BspHI	TCATGA	1 cut(s)	634
BspLI	GGNNCC	3 cut(s)	1224, 2052, 2206
BspMAI	CTGCAG	1 cut(s)	1657
BspMI	ACCTGC	1 cut(s)	160
BspPI	GGATC	5 cut(s)	289, 624, 1460, 1804, 1920
BspT104I	TTCGAA	1 cut(s)	1061
BspT107I	GGYRCC	1 cut(s)	1222
BspTNI	GGTCTC	1 cut(s)	386
BsrDI	GCAATG	2 cut(s)	735, 1142
BsrI	ACTGG	4 cut(s)	259, 1616, 2023, 2067
BssECI	CCNNGG	8 cut(s)	138, 249, 277, 313, 455, 1046, 1251, 1326
BssNI	GRCGYC	3 cut(s)	318, 515, 1223
BssSI	CACGAG	2 cut(s)	641, 1718
BssT1I	CCWWGG	3 cut(s)	138, 249, 1046
Bst2BI	CACGAG	2 cut(s)	641, 1718
Bst2UI	CCWGG	4 cut(s)	528, 1322, 1404, 1713
Bst4CI	ACNGT	4 cut(s)	11, 52, 353, 2266
Bst6I	CTCTTC	2 cut(s)	171, 1620
BstACI	GRCGYC	3 cut(s)	318, 515, 1223
BstBI	TTCGAA	1 cut(s)	1061
BstC8I	GCNNGC	7 cut(s)	277, 459, 843, 1194, 1227, 1732, 2055
BstDEI	CTNAG	8 cut(s)	40, 285, 912, 1008, 1122, 1182, 1242, 1974
BstDSI	CCRYGG	5 cut(s)	138, 249, 277, 455, 1326
BstF5I	GGATG	6 cut(s)	183, 432, 790, 865, 1501, 1535
BstFNI	CGCG	6 cut(s)	29, 279, 372, 457, 473, 1595
BstH2I	RGCGCY	1 cut(s)	1226
BstHHI	GCGC	6 cut(s)	735, 750, 865, 946, 1096, 1225
BstMAI	GTCTC	2 cut(s)	386, 804
BstMWI	GCNNNNNNNGC	8 cut(s)	143, 152, 470, 730, 862, 1139, 2063, 2094
BstNI	CCWGG	4 cut(s)	528, 1322, 1404, 1713
BstNSI	RCATGY	3 cut(s)	607, 748, 1734
BstSCI	CCNGG	7 cut(s)	313, 526, 1251, 1320, 1402, 1711, 2030
BstSFI	CTRYAG	2 cut(s)	1653, 1677
BstUI	CGCG	6 cut(s)	29, 279, 372, 457, 473, 1595
BstX2I	RGATCY	3 cut(s)	281, 616, 1912
BstXI	CCANNNNNNTGG	1 cut(s)	1306
BstYI	RGATCY	3 cut(s)	281, 616, 1912
BsuI	GTATCC	2 cut(s)	1608, 1730
BsuRI	GGCC	5 cut(s)	155, 1325, 1686, 2001, 2066
BtgI	CCRYGG	5 cut(s)	138, 249, 277, 455, 1326
BtgZI	GCGATG	1 cut(s)	1554
BtsCI	GGATG	6 cut(s)	183, 432, 790, 865, 1501, 1535
BtsI	GCAGTG	1 cut(s)	2104
BtsIMutI	CAGTG	2 cut(s)	2060, 2104
BveI	ACCTGC	1 cut(s)	160
Cac8I	GCNNGC	7 cut(s)	277, 459, 843, 1194, 1227, 1732, 2055
CaiI	CAGNNNCTG	2 cut(s)	1652, 1888
CciI	TCATGA	1 cut(s)	634
CfoI	GCGC	6 cut(s)	735, 750, 865, 946, 1096, 1225
Cfr13I	GGNCC	5 cut(s)	289, 775, 1684, 1999, 2105
Cfr42I	CCGCGG	2 cut(s)	280, 458
CseI	GACGC	1 cut(s)	307
Csp6I	GTAC	6 cut(s)	232, 450, 916, 1927, 2035, 2178
CviAII	CATG	8 cut(s)	139, 250, 604, 635, 745, 1391, 1731, 2003
CviQI	GTAC	6 cut(s)	232, 450, 916, 1927, 2035, 2178
DdeI	CTNAG	8 cut(s)	40, 285, 912, 1008, 1122, 1182, 1242, 1974
DinI	GGCGCC	1 cut(s)	1224
DrdI	GACNNNNNNGTC	2 cut(s)	317, 520
DseDI	GACNNNNNNGTC	2 cut(s)	317, 520
EaeI	YGGCCR	2 cut(s)	1323, 2064
Eam1104I	CTCTTC	2 cut(s)	171, 1620
EarI	CTCTTC	2 cut(s)	171, 1620
EciI	GGCGGA	2 cut(s)	46, 713
Eco130I	CCWWGG	3 cut(s)	138, 249, 1046
Eco31I	GGTCTC	1 cut(s)	386
Eco32I	GATATC	2 cut(s)	486, 2019
Eco47I	GGWCC	3 cut(s)	289, 775, 2105
Eco88I	CYCGRG	1 cut(s)	218
EcoO109I	RGGNCCY	2 cut(s)	775, 1684
EcoRI	GAATTC	1 cut(s)	2186
EcoRII	CCWGG	4 cut(s)	526, 1320, 1402, 1711
EcoRV	GATATC	2 cut(s)	486, 2019
EcoT14I	CCWWGG	3 cut(s)	138, 249, 1046
EcoT22I	ATGCAT	1 cut(s)	1570
EgeI	GGCGCC	1 cut(s)	1224
EheI	GGCGCC	1 cut(s)	1224
ErhI	CCWWGG	3 cut(s)	138, 249, 1046
FaeI	CATG	8 cut(s)	142, 253, 607, 638, 748, 1394, 1734, 2006
FaqI	GGGAC	2 cut(s)	920, 2350
FatI	CATG	8 cut(s)	138, 249, 603, 634, 744, 1390, 1730, 2002
FauI	CCCGC	4 cut(s)	284, 365, 450, 473
FauNDI	CATATG	2 cut(s)	2045, 2170
FbaI	TGATCA	2 cut(s)	760, 1498
FokI	GGATG	6 cut(s)	170, 439, 797, 872, 1508, 1522
FspBI	CTAG	2 cut(s)	627, 2256
FspI	TGCGCA	2 cut(s)	864, 945
GlaI	GCGC	6 cut(s)	734, 749, 864, 945, 1095, 1224
GsuI	CTGGAG	2 cut(s)	746, 1155
HaeII	RGCGCY	1 cut(s)	1226
HaeIII	GGCC	5 cut(s)	155, 1325, 1686, 2001, 2066
HapII	CCGG	4 cut(s)	315, 1253, 1357, 2031
HgaI	GACGC	1 cut(s)	307
HhaI	GCGC	6 cut(s)	735, 750, 865, 946, 1096, 1225
Hin1I	GRCGYC	3 cut(s)	318, 515, 1223
Hin1II	CATG	8 cut(s)	142, 253, 607, 638, 748, 1394, 1734, 2006
Hin6I	GCGC	6 cut(s)	733, 748, 863, 944, 1094, 1223
HinP1I	GCGC	6 cut(s)	733, 748, 863, 944, 1094, 1223
HincII	GTYRAC	4 cut(s)	48, 310, 1041, 1804
HindII	GTYRAC	4 cut(s)	48, 310, 1041, 1804
HindIII	AAGCTT	1 cut(s)	1704
HpaI	GTTAAC	2 cut(s)	1041, 1804
HpaII	CCGG	4 cut(s)	315, 1253, 1357, 2031
HphI	GGTGA	5 cut(s)	1098, 1418, 1465, 1712, 2231
Hpy166II	GTNNAC	6 cut(s)	48, 310, 507, 1041, 1804, 1927
Hpy8I	GTNNAC	6 cut(s)	48, 310, 507, 1041, 1804, 1927
Hpy99I	CGWCG	5 cut(s)	323, 502, 517, 520, 883
HpyAV	CCTTC	7 cut(s)	84, 279, 424, 607, 961, 1015, 1697
HpyCH4III	ACNGT	4 cut(s)	11, 52, 353, 2266
HpyCH4IV	ACGT	4 cut(s)	515, 881, 1457, 1640
HpyF10VI	GCNNNNNNNGC	8 cut(s)	143, 152, 470, 730, 862, 1139, 2063, 2094
HpyF3I	CTNAG	8 cut(s)	40, 285, 912, 1008, 1122, 1182, 1242, 1974
HpySE526I	ACGT	4 cut(s)	515, 881, 1457, 1640
Hsp92I	GRCGYC	3 cut(s)	318, 515, 1223
Hsp92II	CATG	8 cut(s)	142, 253, 607, 638, 748, 1394, 1734, 2006
HspAI	GCGC	6 cut(s)	733, 748, 863, 944, 1094, 1223
KasI	GGCGCC	1 cut(s)	1222
Ksp22I	TGATCA	2 cut(s)	760, 1498
KspAI	GTTAAC	2 cut(s)	1041, 1804
KspI	CCGCGG	2 cut(s)	280, 458
LmnI	GCTCC	7 cut(s)	243, 1136, 1595, 1817, 2050, 2078, 2210
LweI	GCATC	8 cut(s)	558, 775, 800, 850, 931, 1228, 1495, 1577
MaeI	CTAG	2 cut(s)	627, 2256
MaeII	ACGT	4 cut(s)	515, 881, 1457, 1640
MaeIII	GTNAC	6 cut(s)	11, 1435, 1453, 1668, 1840, 2158
MboII	GAAGA	8 cut(s)	69, 188, 622, 1626, 1637, 1957, 1982, 1996
MfeI	CAATTG	3 cut(s)	659, 951, 1540
MflI	RGATCY	3 cut(s)	281, 616, 1912
MhlI	GDGCHC	2 cut(s)	542, 642
MlsI	TGGCCA	1 cut(s)	2066
MluNI	TGGCCA	1 cut(s)	2066
Mly113I	GGCGCC	1 cut(s)	1223
MlyI	GAGTC	1 cut(s)	1019
Mox20I	TGGCCA	1 cut(s)	2066
Mph1103I	ATGCAT	1 cut(s)	1570
MroXI	GAANNNNTTC	1 cut(s)	1707
MscI	TGGCCA	1 cut(s)	2066
MseI	TTAA	4 cut(s)	1040, 1083, 1386, 1803
Msp20I	TGGCCA	1 cut(s)	2066
MspA1I	CMGCKG	4 cut(s)	279, 457, 480, 1761
MspI	CCGG	4 cut(s)	315, 1253, 1357, 2031
MspR9I	CCNGG	7 cut(s)	315, 528, 1253, 1322, 1404, 1713, 2032
MunI	CAATTG	3 cut(s)	659, 951, 1540
MvaI	CCWGG	4 cut(s)	528, 1322, 1404, 1713
MvnI	CGCG	6 cut(s)	29, 279, 372, 457, 473, 1595
MwoI	GCNNNNNNNGC	8 cut(s)	143, 152, 470, 730, 862, 1139, 2063, 2094
NarI	GGCGCC	1 cut(s)	1223
NciI	CCSGG	3 cut(s)	315, 1253, 2032
NcoI	CCATGG	2 cut(s)	138, 249
NdeI	CATATG	2 cut(s)	2045, 2170
NlaIII	CATG	8 cut(s)	142, 253, 607, 638, 748, 1394, 1734, 2006
NlaIV	GGNNCC	3 cut(s)	1224, 2052, 2206
NmuCI	GTSAC	3 cut(s)	11, 1453, 2158
NsbI	TGCGCA	2 cut(s)	864, 945
NsiI	ATGCAT	1 cut(s)	1570
NspI	RCATGY	3 cut(s)	607, 748, 1734
NspV	TTCGAA	1 cut(s)	1061
PaeI	GCATGC	1 cut(s)	1734
PaeR7I	CTCGAG	1 cut(s)	218
PagI	TCATGA	1 cut(s)	634
PaqCI	CACCTGC	1 cut(s)	160
PcsI	WCGNNNNNNNCGW	2 cut(s)	494, 887
PdmI	GAANNNNTTC	1 cut(s)	1707
PfoI	TCCNGGA	1 cut(s)	1711
PleI	GAGTC	1 cut(s)	1018
PluTI	GGCGCC	1 cut(s)	1226
PpsI	GAGTC	1 cut(s)	1018
PpuMI	RGGWCCY	1 cut(s)	775
PshBI	ATTAAT	1 cut(s)	1386
Psp5II	RGGWCCY	1 cut(s)	775
Psp6I	CCWGG	4 cut(s)	526, 1320, 1402, 1711
PspGI	CCWGG	4 cut(s)	526, 1320, 1402, 1711
PspN4I	GGNNCC	3 cut(s)	1224, 2052, 2206
PspPI	GGNCC	5 cut(s)	289, 775, 1684, 1999, 2105
PspPPI	RGGWCCY	1 cut(s)	775
PstI	CTGCAG	1 cut(s)	1657
PstNI	CAGNNNCTG	2 cut(s)	1652, 1888
PsuI	RGATCY	3 cut(s)	281, 616, 1912
PvuII	CAGCTG	1 cut(s)	1761
RsaI	GTAC	6 cut(s)	233, 451, 917, 1928, 2036, 2179
RsaNI	GTAC	6 cut(s)	232, 450, 916, 1927, 2035, 2178
SacII	CCGCGG	2 cut(s)	280, 458
SaqAI	TTAA	4 cut(s)	1040, 1083, 1386, 1803
Sau96I	GGNCC	5 cut(s)	289, 775, 1684, 1999, 2105
ScaI	AGTACT	1 cut(s)	917
SchI	GAGTC	1 cut(s)	1019
ScrFI	CCNGG	7 cut(s)	315, 528, 1253, 1322, 1404, 1713, 2032
SduI	GDGCHC	2 cut(s)	542, 642
SfaNI	GCATC	8 cut(s)	558, 775, 800, 850, 931, 1228, 1495, 1577
SfcI	CTRYAG	2 cut(s)	1653, 1677
SfoI	GGCGCC	1 cut(s)	1224
Sfr274I	CTCGAG	1 cut(s)	218
Sfr303I	CCGCGG	2 cut(s)	280, 458
SfuI	TTCGAA	1 cut(s)	1061
SgrBI	CCGCGG	2 cut(s)	280, 458
SinI	GGWCC	3 cut(s)	289, 775, 2105
SlaI	CTCGAG	1 cut(s)	218
SmlI	CTYRAG	1 cut(s)	218
SmoI	CTYRAG	1 cut(s)	218
SphI	GCATGC	1 cut(s)	1734
SspDI	GGCGCC	1 cut(s)	1222
SspMI	CTAG	2 cut(s)	627, 2256
StyD4I	CCNGG	7 cut(s)	313, 526, 1251, 1320, 1402, 1711, 2030
StyI	CCWWGG	3 cut(s)	138, 249, 1046
TaaI	ACNGT	4 cut(s)	11, 52, 353, 2266
TaiI	ACGT	4 cut(s)	518, 884, 1460, 1643
TaqII	GACCGA	1 cut(s)	593
TatI	WGTACW	2 cut(s)	915, 2177
TauI	GCSGC	7 cut(s)	62, 268, 408, 411, 464, 1099, 2205
Tru1I	TTAA	4 cut(s)	1040, 1083, 1386, 1803
Tru9I	TTAA	4 cut(s)	1040, 1083, 1386, 1803
TscAI	CASTG	2 cut(s)	2067, 2104
TseFI	GTSAC	3 cut(s)	11, 1453, 2158
Tsp45I	GTSAC	3 cut(s)	11, 1453, 2158
TspDTI	ATGAA	7 cut(s)	146, 443, 623, 1446, 1524, 1851, 2282
TspGWI	ACGGA	8 cut(s)	557, 570, 1117, 1764, 1809, 1830, 2229, 2252
TspRI	CASTG	2 cut(s)	2067, 2104
VpaK11BI	GGWCC	3 cut(s)	289, 775, 2105
VspI	ATTAAT	1 cut(s)	1386
XapI	RAATTY	3 cut(s)	35, 702, 2186
XbaI	TCTAGA	1 cut(s)	2255
XceI	RCATGY	3 cut(s)	607, 748, 1734
XhoI	CTCGAG	1 cut(s)	218
XmnI	GAANNNNTTC	1 cut(s)	1707
XspI	CTAG	2 cut(s)	627, 2256
ZraI	GACGTC	1 cut(s)	516
ZrmI	AGTACT	1 cut(s)	917
Zsp2I	ATGCAT	1 cut(s)	1570

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.