FvH4_2g06310

B3 domain-containing protein Os01g0234100-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0001540
Physical Location & Seq
Reverse (-)
5240545 .. 5244106
3562 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g06310.t1

Sequence Viewer

Length: 666 bp
ATGCATGGAAATGTTAAGGAGAACACCATAGCCTGTTATACCAAGTATGGTCAAATATCAGACCACAATCAAATTGATAGGGAAGTTCTTCATTTGGAAGTTCCGGATGCAATAAGGGTTTCAGAGTCTGTTATCCCTTTCGAAGAAGTGAGCTGCTTTGAAAACTTTATGGTCACAGTCGGTGGTTCAATCATAAATGCTGAGATCTCCAAACACCATTTGATCAAGTACTATGAGCTCTGCCGCAGTCAAAACTCATTTCTTCATGAGCACATTCTTGAGCCTCTTAACTCCAATTTGGTTGCCGGAGTTATTTCAGAAACAGTAAATCTATCAGATGTCATTAGAGCCGGCAAGATTACTACTTCAGAGTGTGATTTTTTGACTTGGGATAAGACTTTGCAAGCATCTGAGATCTTGGACATGAATGTTGGATTCTTGCGTGCCCGGCTGGACAAGCTTGCAAGCCTTGCTTCAAAATCAAAACTGAATAGAGAGGCTATACTTGCAAGAGACCAAGCAGTAAAAGATTTGATAACCCTGGAAGCAAAGTTTTTAAGAGCCAAAGAGGTTATAAATAGGCTCCACCTTGAGATTGAGACTCTAAATACAAGTTCAGAGAACCTTGAGGCTATGTTCAAAGAAGTGGCAAAGGCCCCTTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

222

Amino Acids

24.97

Weight (kDa)

5.52

Isoelectric Point (pI)

36.56

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 575
AccIII TCCGGA 1 cut(s) 103
AciI CCGC 1 cut(s) 244
AcuI CTGAAG 1 cut(s) 351
AfaI GTAC 1 cut(s) 230
AgsI TTSAA 4 cut(s) 161, 189, 477, 640
AjnI CCWGG 1 cut(s) 540
AluBI AGCT 3 cut(s) 153, 238, 460
AluI AGCT 3 cut(s) 153, 238, 460
Alw21I GWGCWC 2 cut(s) 240, 273
Alw26I GTCTC 2 cut(s) 507, 593
AlwNI CAGNNNCTG 1 cut(s) 128
Aor13HI TCCGGA 1 cut(s) 103
AoxI GGCC 1 cut(s) 654
ApeKI GCWGC 1 cut(s) 153
Asp700I GAANNNNTTC 1 cut(s) 87
AspS9I GGNCC 1 cut(s) 655
AsuC2I CCSGG 1 cut(s) 448
AsuII TTCGAA 1 cut(s) 141
BaeGI GKGCMC 1 cut(s) 448
BanII GRGCYC 1 cut(s) 240
Bbv12I GWGCWC 2 cut(s) 240, 273
BbvI GCAGC 1 cut(s) 140
BciT130I CCWGG 1 cut(s) 542
BclI TGATCA 1 cut(s) 222
BcnI CCSGG 1 cut(s) 448
BcoDI GTCTC 2 cut(s) 507, 593
BglII AGATCT 2 cut(s) 204, 414
BisI GCNGC 2 cut(s) 154, 244
BlsI GCNGC 2 cut(s) 155, 245
BmcAI AGTACT 1 cut(s) 230
Bme1390I CCNGG 2 cut(s) 448, 542
BmgT120I GGNCC 1 cut(s) 655
BmiI GGNNCC 2 cut(s) 584, 657
BmrFI CCNGG 2 cut(s) 448, 542
BmsI GCATC 2 cut(s) 97, 416
Bpu14I TTCGAA 1 cut(s) 141
BpuEI CTTGAG 3 cut(s) 299, 611, 647
BpuMI CCSGG 1 cut(s) 448
BsaI GGTCTC 1 cut(s) 507
BsaJI CCNNGG 2 cut(s) 540, 659
BsaWI WCCGGW 1 cut(s) 103
Bse118I RCCGGY 1 cut(s) 350
BseAI TCCGGA 1 cut(s) 103
BseBI CCWGG 1 cut(s) 542
BseDI CCNNGG 2 cut(s) 540, 659
BseGI GGATG 1 cut(s) 112
BseMII CTCAG 2 cut(s) 192, 402
BseSI GKGCMC 1 cut(s) 448
BseXI GCAGC 1 cut(s) 140
BshFI GGCC 1 cut(s) 656
BsiHKAI GWGCWC 2 cut(s) 240, 273
BsiSI CCGG 4 cut(s) 104, 306, 351, 448
BsmAI GTCTC 2 cut(s) 507, 593
BsnI GGCC 1 cut(s) 656
Bso31I GGTCTC 1 cut(s) 507
Bsp119I TTCGAA 1 cut(s) 141
Bsp1286I GDGCHC 3 cut(s) 240, 273, 448
Bsp13I TCCGGA 1 cut(s) 103
Bsp143I GATC 3 cut(s) 204, 222, 414
BspACI CCGC 1 cut(s) 244
BspANI GGCC 1 cut(s) 656
BspCNI CTCAG 2 cut(s) 193, 403
BspEI TCCGGA 1 cut(s) 103
BspHI TCATGA 1 cut(s) 265
BspLI GGNNCC 2 cut(s) 584, 657
BspT104I TTCGAA 1 cut(s) 141
BspTNI GGTCTC 1 cut(s) 507
BsrFI RCCGGY 1 cut(s) 350
BssAI RCCGGY 1 cut(s) 350
BssECI CCNNGG 2 cut(s) 540, 659
BssMI GATC 3 cut(s) 204, 222, 414
BssT1I CCWWGG 1 cut(s) 659
Bst2UI CCWGG 1 cut(s) 542
Bst4CI ACNGT 2 cut(s) 178, 325
BstAPI GCANNNNNTGC 1 cut(s) 470
BstBI TTCGAA 1 cut(s) 141
BstC8I GCNNGC 5 cut(s) 352, 405, 444, 462, 466
BstDEI CTNAG 2 cut(s) 201, 411
BstF5I GGATG 1 cut(s) 112
BstKTI GATC 3 cut(s) 207, 225, 417
BstMAI GTCTC 2 cut(s) 507, 593
BstMBI GATC 3 cut(s) 204, 222, 414
BstMWI GCNNNNNNNGC 4 cut(s) 448, 457, 470, 506
BstNI CCWGG 1 cut(s) 542
BstSCI CCNGG 2 cut(s) 446, 540
BstSLI GKGCMC 1 cut(s) 448
BstV1I GCAGC 1 cut(s) 140
BstX2I RGATCY 2 cut(s) 204, 414
BstYI RGATCY 2 cut(s) 204, 414
BsuRI GGCC 1 cut(s) 656
BtsCI GGATG 1 cut(s) 112
Cac8I GCNNGC 5 cut(s) 352, 405, 444, 462, 466
CaiI CAGNNNCTG 1 cut(s) 128
CciI TCATGA 1 cut(s) 265
Cfr10I RCCGGY 1 cut(s) 350
Cfr13I GGNCC 1 cut(s) 655
Csp6I GTAC 1 cut(s) 229
CviAII CATG 3 cut(s) 5, 266, 424
CviQI GTAC 1 cut(s) 229
DdeI CTNAG 2 cut(s) 201, 411
DpnI GATC 3 cut(s) 206, 224, 416
DpnII GATC 3 cut(s) 204, 222, 414
Ecl136II GAGCTC 1 cut(s) 238
Eco130I CCWWGG 1 cut(s) 659
Eco24I GRGCYC 1 cut(s) 240
Eco31I GGTCTC 1 cut(s) 507
Eco53kI GAGCTC 1 cut(s) 238
Eco57I CTGAAG 1 cut(s) 351
EcoICRI GAGCTC 1 cut(s) 238
EcoO109I RGGNCCY 1 cut(s) 655
EcoRII CCWGG 1 cut(s) 540
EcoT14I CCWWGG 1 cut(s) 659
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 1 cut(s) 240
ErhI CCWWGG 1 cut(s) 659
FaeI CATG 3 cut(s) 8, 269, 427
FalI AAGNNNNNCTT 2 cut(s) 457, 489
FatI CATG 3 cut(s) 4, 265, 423
FbaI TGATCA 1 cut(s) 222
Fnu4HI GCNGC 2 cut(s) 154, 244
FokI GGATG 1 cut(s) 119
FriOI GRGCYC 1 cut(s) 240
Fsp4HI GCNGC 2 cut(s) 154, 244
GluI GCNGC 2 cut(s) 154, 244
HaeIII GGCC 1 cut(s) 656
HapII CCGG 4 cut(s) 104, 306, 351, 448
Hin1II CATG 3 cut(s) 8, 269, 427
HindIII AAGCTT 1 cut(s) 458
HinfI GANTC 3 cut(s) 125, 435, 601
HpaII CCGG 4 cut(s) 104, 306, 351, 448
Hpy188I TCNGA 7 cut(s) 61, 124, 319, 337, 370, 412, 619
Hpy188III TCNNGA 3 cut(s) 104, 266, 278
HpyCH4III ACNGT 2 cut(s) 178, 325
HpyCH4V TGCA 5 cut(s) 4, 110, 403, 464, 509
HpyF10VI GCNNNNNNNGC 4 cut(s) 448, 457, 470, 506
HpyF3I CTNAG 2 cut(s) 201, 411
Hsp92II CATG 3 cut(s) 8, 269, 427
Kpn2I TCCGGA 1 cut(s) 103
KroI GCCGGC 1 cut(s) 350
KroNI GCCGGC 1 cut(s) 352
Ksp22I TGATCA 1 cut(s) 222
Kzo9I GATC 3 cut(s) 204, 222, 414
LmnI GCTCC 1 cut(s) 588
LpnPI CCDG 8 cut(s) 46, 117, 319, 364, 437, 461, 527, 554
Lsp1109I GCAGC 1 cut(s) 140
LweI GCATC 2 cut(s) 97, 416
MaeIII GTNAC 1 cut(s) 172
MalI GATC 3 cut(s) 206, 224, 416
MboI GATC 3 cut(s) 204, 222, 414
MboII GAAGA 3 cut(s) 80, 155, 254
MflI RGATCY 2 cut(s) 204, 414
MhlI GDGCHC 3 cut(s) 240, 273, 448
MluCI AATT 2 cut(s) 72, 295
MlyI GAGTC 2 cut(s) 134, 595
MmeI TCCRAC 1 cut(s) 412
MnlI CCTC 4 cut(s) 294, 490, 562, 622
Mph1103I ATGCAT 1 cut(s) 6
MroI TCCGGA 1 cut(s) 103
MroNI GCCGGC 1 cut(s) 350
MroXI GAANNNNTTC 1 cut(s) 87
MseI TTAA 3 cut(s) 15, 288, 557
MslI CAYNNNNRTG 1 cut(s) 9
MspI CCGG 4 cut(s) 104, 306, 351, 448
MspR9I CCNGG 2 cut(s) 448, 542
MvaI CCWGG 1 cut(s) 542
MwoI GCNNNNNNNGC 4 cut(s) 448, 457, 470, 506
NaeI GCCGGC 1 cut(s) 352
NciI CCSGG 1 cut(s) 448
NdeII GATC 3 cut(s) 204, 222, 414
NgoMIV GCCGGC 1 cut(s) 350
NlaIII CATG 3 cut(s) 8, 269, 427
NlaIV GGNNCC 2 cut(s) 584, 657
NmuCI GTSAC 1 cut(s) 172
NsiI ATGCAT 1 cut(s) 6
NspV TTCGAA 1 cut(s) 141
PagI TCATGA 1 cut(s) 265
PdiI GCCGGC 1 cut(s) 352
PdmI GAANNNNTTC 1 cut(s) 87
PfeI GAWTC 1 cut(s) 435
PkrI GCNGC 2 cut(s) 155, 245
PleI GAGTC 2 cut(s) 133, 595
PpsI GAGTC 2 cut(s) 133, 595
PsiI TTATAA 1 cut(s) 575
Psp124BI GAGCTC 1 cut(s) 240
Psp6I CCWGG 1 cut(s) 540
PspGI CCWGG 1 cut(s) 540
PspN4I GGNNCC 2 cut(s) 584, 657
PspPI GGNCC 1 cut(s) 655
PstNI CAGNNNCTG 1 cut(s) 128
PsuI RGATCY 2 cut(s) 204, 414
RsaI GTAC 1 cut(s) 230
RsaNI GTAC 1 cut(s) 229
RseI CAYNNNNRTG 1 cut(s) 9
SacI GAGCTC 1 cut(s) 240
SaqAI TTAA 3 cut(s) 15, 288, 557
SatI GCNGC 2 cut(s) 154, 244
Sau3AI GATC 3 cut(s) 204, 222, 414
Sau96I GGNCC 1 cut(s) 655
ScaI AGTACT 1 cut(s) 230
SchI GAGTC 2 cut(s) 134, 595
ScrFI CCNGG 2 cut(s) 448, 542
SduI GDGCHC 3 cut(s) 240, 273, 448
SetI ASST 6 cut(s) 155, 240, 462, 573, 591, 627
SfaNI GCATC 2 cut(s) 97, 416
SfuI TTCGAA 1 cut(s) 141
SmiMI CAYNNNNRTG 1 cut(s) 9
SmlI CTYRAG 3 cut(s) 278, 590, 626
SmoI CTYRAG 3 cut(s) 278, 590, 626
Sse9I AATT 2 cut(s) 72, 295
SsiI CCGC 1 cut(s) 244
SstI GAGCTC 1 cut(s) 240
StyD4I CCNGG 2 cut(s) 446, 540
StyI CCWWGG 1 cut(s) 659
TaaI ACNGT 2 cut(s) 178, 325
TaqI TCGA 1 cut(s) 141
TasI AATT 2 cut(s) 72, 295
TatI WGTACW 1 cut(s) 228
TauI GCSGC 1 cut(s) 246
TfiI GAWTC 1 cut(s) 435
Tru1I TTAA 3 cut(s) 15, 288, 557
Tru9I TTAA 3 cut(s) 15, 288, 557
TseFI GTSAC 1 cut(s) 172
TseI GCWGC 1 cut(s) 153
Tsp45I GTSAC 1 cut(s) 172
TspDTI ATGAA 3 cut(s) 80, 254, 440
XmnI GAANNNNTTC 1 cut(s) 87
ZrmI AGTACT 1 cut(s) 230
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.