Rorug05G0577500

B3 domain-containing protein Os01g0234100-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0001540
Physical Location & Seq
Forward (+)
77369042 .. 77371559
2518 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0577500.1

Sequence Viewer

Length: 630 bp
ATGTCACATCATACTGGGGAAACTACCACAAGCGATTTCATTACCAGTTTGATTGCAAATTTAGGGCAAGTCAATAGGTCATCAGTGAACGAAGATGATGCCCATGTCTCACTTAACTGGAAAGATATTGGACTTTCTGTTCCACCAACACTCAAGAGGGCTTTTGGTTGTTGCACAATGCTTGGACCCATGAATACTGAGTATAAGGAAAGGAAGGCTGCTGTCCGGCGTACAAAGCCTACCACGGCTCATCTGCCCACAGAGATTAATGAAGGAGGGGAAACAAATGAGACAGACTTAAACATGTTAACAATGTATGAGATATTAAAGAAGAAGAAGAATGTCGAACTCAGTTGTTTGATACTGAATAGAAGGTCCTTTGCACAGACCGTGGAGAATCTATTCGCTCTTTCATTCCTCGCAAAAGACGGTAGAGTTAGAATTGCCGTCGAAGCAAATGGTTCTCATGTCGTTTCACCTACGAATGGTCCTGTTGCCAATGCAGTGGCTTATCACCATTTTGTGTTCCGATTTGATTTCAAGGACTGGAAGATAATGACTGAAATGGTGGCTGTTGGTGAGGAGCTGATGCCTCATAGAGACCACACAAAGTGCACACCTGCCCCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

209

Amino Acids

23.33

Weight (kDa)

7.12

Isoelectric Point (pI)

39.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nse4_C PF08743 115 - 197 2.2e-20 Nse4 C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 58
AdeI CACNNNGTG 1 cut(s) 612
AfaI GTAC 1 cut(s) 232
AfiI CCNNNNNNNGG 1 cut(s) 485
AflIII ACRYGT 1 cut(s) 303
AgsI TTSAA 1 cut(s) 541
AloI GAACNNNNNNTCC 2 cut(s) 123, 155
AluBI AGCT 1 cut(s) 586
AluI AGCT 1 cut(s) 586
Alw21I GWGCWC 1 cut(s) 617
Alw26I GTCTC 3 cut(s) 112, 284, 594
Alw44I GTGCAC 1 cut(s) 613
ApaLI GTGCAC 1 cut(s) 613
ApeKI GCWGC 1 cut(s) 218
ApoI RAATTY 1 cut(s) 58
AseI ATTAAT 1 cut(s) 267
Asp700I GAANNNNTTC 1 cut(s) 401
AspS9I GGNCC 3 cut(s) 185, 375, 488
AsuHPI GGTGA 3 cut(s) 468, 506, 590
AvaII GGWCC 3 cut(s) 185, 375, 488
BaeGI GKGCMC 1 cut(s) 617
Bbv12I GWGCWC 1 cut(s) 617
BbvI GCAGC 1 cut(s) 205
BceAI ACGGC 2 cut(s) 261, 431
BcgI CGANNNNNNTGC 2 cut(s) 80, 114
BcoDI GTCTC 3 cut(s) 112, 284, 594
BisI GCNGC 1 cut(s) 219
BlsI GCNGC 1 cut(s) 220
Bme18I GGWCC 3 cut(s) 185, 375, 488
BmgT120I GGNCC 3 cut(s) 185, 375, 488
BmiI GGNNCC 1 cut(s) 187
BmrI ACTGGG 1 cut(s) 24
BmsI GCATC 2 cut(s) 88, 579
BmuI ACTGGG 1 cut(s) 24
BpuEI CTTGAG 1 cut(s) 137
BsaI GGTCTC 1 cut(s) 594
BsaJI CCNNGG 2 cut(s) 243, 390
Bsc4I CCNNNNNNNGG 1 cut(s) 485
Bse1I ACTGG 4 cut(s) 19, 45, 122, 551
BseDI CCNNGG 2 cut(s) 243, 390
BseLI CCNNNNNNNGG 1 cut(s) 485
BseMII CTCAG 2 cut(s) 189, 364
BseNI ACTGG 4 cut(s) 19, 45, 122, 551
BseRI GAGGAG 1 cut(s) 596
BseSI GKGCMC 1 cut(s) 617
BseXI GCAGC 1 cut(s) 205
BsiHKAI GWGCWC 1 cut(s) 617
BsiSI CCGG 1 cut(s) 226
BslI CCNNNNNNNGG 1 cut(s) 485
BsmAI GTCTC 3 cut(s) 112, 284, 594
Bso31I GGTCTC 1 cut(s) 594
Bsp1286I GDGCHC 1 cut(s) 617
BspCNI CTCAG 2 cut(s) 190, 363
BspLI GGNNCC 1 cut(s) 187
BspTNI GGTCTC 1 cut(s) 594
BsrI ACTGG 4 cut(s) 19, 45, 122, 551
BssECI CCNNGG 2 cut(s) 243, 390
Bst4CI ACNGT 2 cut(s) 391, 431
BstDEI CTNAG 3 cut(s) 198, 350, 627
BstDSI CCRYGG 2 cut(s) 243, 390
BstMAI GTCTC 3 cut(s) 112, 284, 594
BstMWI GCNNNNNNNGC 2 cut(s) 235, 452
BstNSI RCATGY 1 cut(s) 307
BstSLI GKGCMC 1 cut(s) 617
BstV1I GCAGC 1 cut(s) 205
BstXI CCANNNNNNTGG 1 cut(s) 505
BtgI CCRYGG 2 cut(s) 243, 390
BtsI GCAGTG 1 cut(s) 510
BtsIMutI CAGTG 2 cut(s) 90, 510
Cfr13I GGNCC 3 cut(s) 185, 375, 488
Csp6I GTAC 1 cut(s) 231
CviAII CATG 4 cut(s) 104, 190, 304, 467
CviJI RGCY 7 cut(s) 161, 218, 238, 248, 509, 572, 586
CviKI_1 RGCY 7 cut(s) 161, 218, 238, 248, 509, 572, 586
CviQI GTAC 1 cut(s) 231
DdeI CTNAG 3 cut(s) 198, 350, 627
DraIII CACNNNGTG 1 cut(s) 612
Eco31I GGTCTC 1 cut(s) 594
Eco47I GGWCC 3 cut(s) 185, 375, 488
EcoO109I RGGNCCY 1 cut(s) 375
FaeI CATG 4 cut(s) 107, 193, 307, 470
FaiI YATR 8 cut(s) 12, 105, 191, 204, 305, 318, 468, 597
FatI CATG 4 cut(s) 103, 189, 303, 466
Fnu4HI GCNGC 1 cut(s) 219
Fsp4HI GCNGC 1 cut(s) 219
GluI GCNGC 1 cut(s) 219
HapII CCGG 1 cut(s) 226
Hin1II CATG 4 cut(s) 107, 193, 307, 470
HincII GTYRAC 1 cut(s) 309
HindII GTYRAC 1 cut(s) 309
HinfI GANTC 1 cut(s) 397
HpaI GTTAAC 1 cut(s) 309
HpaII CCGG 1 cut(s) 226
HphI GGTGA 3 cut(s) 468, 506, 590
Hpy166II GTNNAC 3 cut(s) 88, 309, 615
Hpy188I TCNGA 1 cut(s) 530
Hpy188III TCNNGA 1 cut(s) 154
Hpy8I GTNNAC 3 cut(s) 88, 309, 615
Hpy99I CGWCG 1 cut(s) 452
HpyAV CCTTC 3 cut(s) 208, 266, 366
HpyCH4III ACNGT 2 cut(s) 391, 431
HpyCH4V TGCA 5 cut(s) 56, 174, 383, 503, 615
HpyF10VI GCNNNNNNNGC 2 cut(s) 235, 452
HpyF3I CTNAG 3 cut(s) 198, 350, 627
Hsp92II CATG 4 cut(s) 107, 193, 307, 470
KspAI GTTAAC 1 cut(s) 309
LmnI GCTCC 1 cut(s) 583
LpnPI CCDG 5 cut(s) 58, 103, 239, 504, 532
Lsp1109I GCAGC 1 cut(s) 205
LweI GCATC 2 cut(s) 88, 579
MaeIII GTNAC 1 cut(s) 3
MboII GAAGA 5 cut(s) 104, 343, 346, 349, 562
MhlI GDGCHC 1 cut(s) 617
MluCI AATT 2 cut(s) 58, 441
MnlI CCTC 5 cut(s) 150, 269, 428, 574, 603
MroXI GAANNNNTTC 1 cut(s) 401
MseI TTAA 5 cut(s) 114, 267, 299, 308, 326
MspI CCGG 1 cut(s) 226
MwoI GCNNNNNNNGC 2 cut(s) 235, 452
NlaIII CATG 4 cut(s) 107, 193, 307, 470
NlaIV GGNNCC 1 cut(s) 187
NmuCI GTSAC 1 cut(s) 3
NspI RCATGY 1 cut(s) 307
PciI ACATGT 1 cut(s) 303
PdmI GAANNNNTTC 1 cut(s) 401
PfeI GAWTC 1 cut(s) 397
PkrI GCNGC 1 cut(s) 220
PpuMI RGGWCCY 1 cut(s) 375
PscI ACATGT 1 cut(s) 303
PshBI ATTAAT 1 cut(s) 267
Psp5II RGGWCCY 1 cut(s) 375
PspN4I GGNNCC 1 cut(s) 187
PspPI GGNCC 3 cut(s) 185, 375, 488
PspPPI RGGWCCY 1 cut(s) 375
RsaI GTAC 1 cut(s) 232
RsaNI GTAC 1 cut(s) 231
SaqAI TTAA 5 cut(s) 114, 267, 299, 308, 326
SatI GCNGC 1 cut(s) 219
Sau96I GGNCC 3 cut(s) 185, 375, 488
SduI GDGCHC 1 cut(s) 617
SetI ASST 5 cut(s) 80, 377, 481, 588, 622
SfaNI GCATC 2 cut(s) 88, 579
SinI GGWCC 3 cut(s) 185, 375, 488
SmlI CTYRAG 1 cut(s) 152
SmoI CTYRAG 1 cut(s) 152
Sse9I AATT 2 cut(s) 58, 441
TaaI ACNGT 2 cut(s) 391, 431
TaqI TCGA 2 cut(s) 345, 450
TasI AATT 2 cut(s) 58, 441
TfiI GAWTC 1 cut(s) 397
Tru1I TTAA 5 cut(s) 114, 267, 299, 308, 326
Tru9I TTAA 5 cut(s) 114, 267, 299, 308, 326
TscAI CASTG 2 cut(s) 90, 510
TseFI GTSAC 1 cut(s) 3
TseI GCWGC 1 cut(s) 218
Tsp45I GTSAC 1 cut(s) 3
TspDTI ATGAA 4 cut(s) 28, 206, 285, 402
TspRI CASTG 2 cut(s) 90, 510
VneI GTGCAC 1 cut(s) 613
VpaK11BI GGWCC 3 cut(s) 185, 375, 488
VspI ATTAAT 1 cut(s) 267
XapI RAATTY 1 cut(s) 58
XceI RCATGY 1 cut(s) 307
XmnI GAANNNNTTC 1 cut(s) 401
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.