Prupe.8G122700_v2.0.a1

B3 domain-containing protein Os01g0234100-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0001540
Physical Location & Seq
Forward (+)
14823514 .. 14831809
8296 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G122700.3

Sequence Viewer

Length: 1236 bp
ATGGCTGTTCTTCAAAACCAGAAACATAGCTACTCCTCTGACACTCCACTACCATCCAAGAGCAATAGGGGAAGACTACCAAAGGACAAGGAAAAGCTGTTTAGCAAGAAATATTCATGTCTCACTCAGTCACAGATCACTAAAAGCATTGTAGTCAGTGCTAATCCAAAGATGCCTGAAAGTCGTAAATTGAAGAGAGCTACAGTAGAAAGCTTATATGATACTTCCCAAGCTCAGTCTTCTGTTCTGCAAAGAGCAAAGGAGGTTGAAGCAAGCCTAGATCCGAAGTTCCCTACCCTACTCAAAGTTATGCTCCCATCACATGTTACTGGATGTTTCTGGCTTGGTCTTCCTAAGAAATTCTGCTGTGATCATTTGCTAAAACAAGATACAATGATTGTTCTGGAAGATGAAAATGGGGAAGAATTTGAAACGAAATATCTTGCGGAGAAGGTAGGACTGAGTGGTGGATGGAGAGGGTTTTCAATGTCTCACAAGCTACTGGAGGAAGATATTGTGATTTTACATGTAGTCACTCCTTCCAAATTCAAGGTTTATATTATTAGATCAAATGGTTTAGATGAAGCGGATTGCGCTGTTGGCCTCACGAGGTTAGATGCTGGCATTAAGCAGATGGATACTGGACACATTATTTCATGTGTAAGGGAAGAAAGTGGGAATTTGGAACCTATTCCAGAGGACTATGCAATAGCCTTTAATACAAAATCTCGTCCACTATCAGACCAGTCTGAAAATGATAGTGAAGATCTTGGTTTTGAAGTTCTGGATGGACTTAGACTTTCAGAGTCTGTTGTCCCATTCAAAGAAATGTACCGTATGGATAACTTCAACGTCTTGGTCAATGGTTTAAACATAAATTCCGAGTTTTCGAAATACGTTCTGAACAAGTATTACGAGCTTTGCTGCAGTCAAAACTCATTCCTTCATGAACATCTTCTTGAGGGTCTGAACTGCAAACTGATAGTTGGAGTAACTTCTGAGACAATCAACATTGCAGATGCCATCAGGGCCTGCAAGATTACAACCTCAGAAGGTTACTTTTCCACTTGGAATAAGACTTTGATAGCCTTTGAGGGTTTAGGCATGAATGTCGGGTTCCTGCGTGCTCGGCTTGACCAGCTGGCAATTCTTGCTTCAAAATCAAAAGGATTTAAGGAGGCTAGACTTGAAGAGCTTAGAGGCAAAGCTTTTGGAAGTAAAAGAGACCATAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

412

Amino Acids

46.13

Weight (kDa)

7.09

Isoelectric Point (pI)

42.97

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 446, 587
AclWI GGATC 1 cut(s) 275
AcsI RAATTY 5 cut(s) 359, 425, 545, 679, 877
AfaI GTAC 1 cut(s) 833
AflIII ACRYGT 2 cut(s) 322, 526
Alw21I GWGCWC 1 cut(s) 1129
Alw26I GTCTC 4 cut(s) 125, 495, 995, 1218
AlwI GGATC 1 cut(s) 275
AlwNI CAGNNNCTG 2 cut(s) 809, 1032
AoxI GGCC 2 cut(s) 601, 1029
ApeKI GCWGC 1 cut(s) 924
ApoI RAATTY 5 cut(s) 359, 425, 545, 679, 877
Asp700I GAANNNNTTC 2 cut(s) 690, 954
AspLEI GCGC 1 cut(s) 596
AspS9I GGNCC 1 cut(s) 1029
AsuII TTCGAA 1 cut(s) 890
BauI CACGAG 1 cut(s) 607
BbsI GAAGAC 3 cut(s) 79, 231, 341
Bbv12I GWGCWC 1 cut(s) 1129
BbvI GCAGC 1 cut(s) 911
BccI CCATC 6 cut(s) 61, 325, 465, 628, 782, 1031
BcgI CGANNNNNNTGC 2 cut(s) 1093, 1127
BciVI GTATCC 1 cut(s) 631
BclI TGATCA 1 cut(s) 370
BcoDI GTCTC 4 cut(s) 125, 495, 995, 1218
BfaI CTAG 2 cut(s) 278, 1182
BfmI CTRYAG 2 cut(s) 201, 925
BfuI GTATCC 1 cut(s) 631
BglI GCCNNNNNGGC 1 cut(s) 1028
BglII AGATCT 1 cut(s) 766
BisI GCNGC 1 cut(s) 925
BlsI GCNGC 1 cut(s) 926
BmgT120I GGNCC 1 cut(s) 1029
BmiI GGNNCC 2 cut(s) 687, 1118
BmsI GCATC 3 cut(s) 162, 607, 1009
BpiI GAAGAC 3 cut(s) 79, 231, 341
BpmI CTGGAG 1 cut(s) 524
Bpu14I TTCGAA 1 cut(s) 890
BpuEI CTTGAG 1 cut(s) 980
BsaI GGTCTC 1 cut(s) 1218
Bse1I ACTGG 4 cut(s) 334, 507, 646, 745
Bse3DI GCAATG 1 cut(s) 1011
BseGI GGATG 4 cut(s) 53, 338, 476, 793
BseMI GCAATG 1 cut(s) 1011
BseMII CTCAG 5 cut(s) 140, 248, 452, 990, 1062
BseNI ACTGG 4 cut(s) 334, 507, 646, 745
BseRI GAGGAG 1 cut(s) 25
BseXI GCAGC 1 cut(s) 911
BshFI GGCC 2 cut(s) 603, 1031
BsiHKAI GWGCWC 1 cut(s) 1129
BslFI GGGAC 1 cut(s) 800
BsmAI GTCTC 4 cut(s) 125, 495, 995, 1218
BsmFI GGGAC 1 cut(s) 800
BsnI GGCC 2 cut(s) 603, 1031
Bso31I GGTCTC 1 cut(s) 1218
Bsp119I TTCGAA 1 cut(s) 890
Bsp1286I GDGCHC 1 cut(s) 1129
Bsp143I GATC 5 cut(s) 135, 280, 370, 566, 766
BspACI CCGC 2 cut(s) 446, 587
BspANI GGCC 2 cut(s) 603, 1031
BspCNI CTCAG 5 cut(s) 139, 247, 453, 991, 1061
BspHI TCATGA 1 cut(s) 946
BspLI GGNNCC 2 cut(s) 687, 1118
BspMAI CTGCAG 1 cut(s) 929
BspPI GGATC 1 cut(s) 275
BspQI GCTCTTC 1 cut(s) 1185
BspT104I TTCGAA 1 cut(s) 890
BspTNI GGTCTC 1 cut(s) 1218
BsrDI GCAATG 1 cut(s) 1011
BsrI ACTGG 4 cut(s) 334, 507, 646, 745
BssMI GATC 5 cut(s) 135, 280, 370, 566, 766
BssSI CACGAG 1 cut(s) 607
Bst2BI CACGAG 1 cut(s) 607
Bst4CI ACNGT 2 cut(s) 205, 836
Bst6I CTCTTC 2 cut(s) 188, 1185
BstAPI GCANNNNNTGC 1 cut(s) 1151
BstBI TTCGAA 1 cut(s) 890
BstC8I GCNNGC 5 cut(s) 274, 622, 1033, 1125, 1143
BstDEI CTNAG 8 cut(s) 126, 234, 354, 461, 794, 999, 1048, 1196
BstF5I GGATG 4 cut(s) 53, 338, 476, 793
BstHHI GCGC 1 cut(s) 596
BstKTI GATC 5 cut(s) 138, 283, 373, 569, 769
BstMAI GTCTC 4 cut(s) 125, 495, 995, 1218
BstMBI GATC 5 cut(s) 135, 280, 370, 566, 766
BstMWI GCNNNNNNNGC 6 cut(s) 593, 600, 1028, 1129, 1138, 1151
BstNSI RCATGY 2 cut(s) 326, 530
BstSFI CTRYAG 2 cut(s) 201, 925
BstV1I GCAGC 1 cut(s) 911
BstV2I GAAGAC 3 cut(s) 79, 231, 341
BstX2I RGATCY 2 cut(s) 280, 766
BstYI RGATCY 2 cut(s) 280, 766
BsuI GTATCC 1 cut(s) 631
BsuRI GGCC 2 cut(s) 603, 1031
BtsCI GGATG 4 cut(s) 53, 338, 476, 793
BtsIMutI CAGTG 1 cut(s) 163
Cac8I GCNNGC 5 cut(s) 274, 622, 1033, 1125, 1143
CaiI CAGNNNCTG 2 cut(s) 809, 1032
CciI TCATGA 1 cut(s) 946
CfoI GCGC 1 cut(s) 596
Cfr13I GGNCC 1 cut(s) 1029
Csp6I GTAC 1 cut(s) 832
CviAII CATG 6 cut(s) 117, 323, 527, 657, 947, 1105
CviQI GTAC 1 cut(s) 832
DdeI CTNAG 8 cut(s) 126, 234, 354, 461, 794, 999, 1048, 1196
DpnI GATC 5 cut(s) 137, 282, 372, 568, 768
DpnII GATC 5 cut(s) 135, 280, 370, 566, 766
DraI TTTAAA 1 cut(s) 870
Eam1104I CTCTTC 2 cut(s) 188, 1185
EarI CTCTTC 2 cut(s) 188, 1185
Eco31I GGTCTC 1 cut(s) 1218
EcoO109I RGGNCCY 1 cut(s) 1029
FaeI CATG 6 cut(s) 120, 326, 530, 660, 950, 1108
FaqI GGGAC 1 cut(s) 800
FatI CATG 6 cut(s) 116, 322, 526, 656, 946, 1104
FbaI TGATCA 1 cut(s) 370
Fnu4HI GCNGC 1 cut(s) 925
FokI GGATG 4 cut(s) 40, 345, 483, 800
Fsp4HI GCNGC 1 cut(s) 925
FspBI CTAG 2 cut(s) 278, 1182
GlaI GCGC 1 cut(s) 595
GluI GCNGC 1 cut(s) 925
GsuI CTGGAG 1 cut(s) 524
HaeIII GGCC 2 cut(s) 603, 1031
HhaI GCGC 1 cut(s) 596
Hin1II CATG 6 cut(s) 120, 326, 530, 660, 950, 1108
Hin6I GCGC 1 cut(s) 594
HinP1I GCGC 1 cut(s) 594
HindIII AAGCTT 2 cut(s) 211, 1206
HinfI GANTC 1 cut(s) 806
Hpy166II GTNNAC 1 cut(s) 734
Hpy188III TCNNGA 6 cut(s) 404, 607, 695, 785, 947, 959
Hpy8I GTNNAC 1 cut(s) 734
HpyAV CCTTC 4 cut(s) 445, 549, 953, 1046
HpyCH4III ACNGT 2 cut(s) 205, 836
HpyCH4IV ACGT 2 cut(s) 852, 897
HpyCH4V TGCA 6 cut(s) 250, 707, 927, 975, 1016, 1035
HpyF10VI GCNNNNNNNGC 6 cut(s) 593, 600, 1028, 1129, 1138, 1151
HpyF3I CTNAG 8 cut(s) 126, 234, 354, 461, 794, 999, 1048, 1196
HpySE526I ACGT 2 cut(s) 852, 897
Hsp92II CATG 6 cut(s) 120, 326, 530, 660, 950, 1108
HspAI GCGC 1 cut(s) 594
Ksp22I TGATCA 1 cut(s) 370
Kzo9I GATC 5 cut(s) 135, 280, 370, 566, 766
LguI GCTCTTC 1 cut(s) 1185
LmnI GCTCC 1 cut(s) 318
Lsp1109I GCAGC 1 cut(s) 911
LweI GCATC 3 cut(s) 162, 607, 1009
MaeI CTAG 2 cut(s) 278, 1182
MaeII ACGT 2 cut(s) 852, 897
MaeIII GTNAC 5 cut(s) 129, 325, 532, 991, 1055
MalI GATC 5 cut(s) 137, 282, 372, 568, 768
MboI GATC 5 cut(s) 135, 280, 370, 566, 766
MflI RGATCY 2 cut(s) 280, 766
MhlI GDGCHC 1 cut(s) 1129
MluCI AATT 7 cut(s) 188, 359, 425, 545, 679, 877, 1146
MlyI GAGTC 1 cut(s) 815
MmeI TCCRAC 1 cut(s) 967
MroXI GAANNNNTTC 2 cut(s) 690, 954
MseI TTAA 4 cut(s) 627, 717, 869, 1173
MspA1I CMGCKG 1 cut(s) 1141
MssI GTTTAAAC 1 cut(s) 870
MwoI GCNNNNNNNGC 6 cut(s) 593, 600, 1028, 1129, 1138, 1151
NdeII GATC 5 cut(s) 135, 280, 370, 566, 766
NlaIII CATG 6 cut(s) 120, 326, 530, 660, 950, 1108
NlaIV GGNNCC 2 cut(s) 687, 1118
NmeAIII GCCGAG 1 cut(s) 1108
NmuCI GTSAC 2 cut(s) 129, 532
NspI RCATGY 2 cut(s) 326, 530
NspV TTCGAA 1 cut(s) 890
PagI TCATGA 1 cut(s) 946
PciI ACATGT 2 cut(s) 322, 526
PciSI GCTCTTC 1 cut(s) 1185
PdmI GAANNNNTTC 2 cut(s) 690, 954
PkrI GCNGC 1 cut(s) 926
PleI GAGTC 1 cut(s) 814
PmeI GTTTAAAC 1 cut(s) 870
PpsI GAGTC 1 cut(s) 814
PscI ACATGT 2 cut(s) 322, 526
PspN4I GGNNCC 2 cut(s) 687, 1118
PspPI GGNCC 1 cut(s) 1029
PsrI GAACNNNNNNTAC 2 cut(s) 896, 928
PstI CTGCAG 1 cut(s) 929
PstNI CAGNNNCTG 2 cut(s) 809, 1032
PsuI RGATCY 2 cut(s) 280, 766
PvuII CAGCTG 1 cut(s) 1141
RsaI GTAC 1 cut(s) 833
RsaNI GTAC 1 cut(s) 832
SapI GCTCTTC 1 cut(s) 1185
SaqAI TTAA 4 cut(s) 627, 717, 869, 1173
SatI GCNGC 1 cut(s) 925
Sau3AI GATC 5 cut(s) 135, 280, 370, 566, 766
Sau96I GGNCC 1 cut(s) 1029
SchI GAGTC 1 cut(s) 815
SduI GDGCHC 1 cut(s) 1129
SfaNI GCATC 3 cut(s) 162, 607, 1009
SfcI CTRYAG 2 cut(s) 201, 925
SfuI TTCGAA 1 cut(s) 890
SmlI CTYRAG 1 cut(s) 959
SmoI CTYRAG 1 cut(s) 959
Sse9I AATT 7 cut(s) 188, 359, 425, 545, 679, 877, 1146
SsiI CCGC 2 cut(s) 446, 587
SspI AATATT 1 cut(s) 113
SspMI CTAG 2 cut(s) 278, 1182
TaaI ACNGT 2 cut(s) 205, 836
TaiI ACGT 2 cut(s) 855, 900
TaqI TCGA 1 cut(s) 890
TasI AATT 7 cut(s) 188, 359, 425, 545, 679, 877, 1146
Tru1I TTAA 4 cut(s) 627, 717, 869, 1173
Tru9I TTAA 4 cut(s) 627, 717, 869, 1173
TscAI CASTG 1 cut(s) 163
TseFI GTSAC 2 cut(s) 129, 532
TseI GCWGC 1 cut(s) 924
Tsp45I GTSAC 2 cut(s) 129, 532
TspDTI ATGAA 7 cut(s) 105, 426, 597, 645, 935, 963, 1121
TspRI CASTG 1 cut(s) 163
XapI RAATTY 5 cut(s) 359, 425, 545, 679, 877
XceI RCATGY 2 cut(s) 326, 530
XmnI GAANNNNTTC 2 cut(s) 690, 954
XspI CTAG 2 cut(s) 278, 1182
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.