Prupe.8G122800_v2.0.a1

B3 domain-containing protein Os01g0234100-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0001540
Physical Location & Seq
Forward (+)
14835235 .. 14839215
3981 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G122800.3

Sequence Viewer

Length: 1356 bp
ATGGCTGTTCTTCAAGACCGGAAACAGAGCTCCTCTGACCTACCACCACCCAAGAGTAATAGGCGACGACCACCAAGGAACAAGGAAAATATGTTTAGGAAGAAATCTTCATCTCTCACTCAGTCACAGACGACCAAAAGCATTGTAGTCAGTGCTAATCCAATGATGCCTGAAAGTCATAAACGAAAGAGAGCCACAGTGGAAAGCATATATGATACTTCCCAAGCTCAGTCTTCTGTTCTGGAAAGAGCACAGGAGGTTGAAGTAAGCCTAGATCCTAAGTTCCCTAGCCTACTCAAAGTTATGCTTCCATCACATGTTACTGGGGGATTCTGGCTGGGTCTTCCTAAGAAATTCTGCTGTGAGCATTTGCCAAAACAGGATATAATGATTGCTCTGGAAGATGAAAATGGGGAGGTATTCGAAACAAAATATCTGGCGGATAAGGTGGGACTGAGTGGTGGATGGAGAGGGTTTTCAATTGCTCACAAGCTACTGGAAGGAGATGTTATAGTTTTCCATCTAGTCACTCCTTCCAAATTTAAGGTGTATATTGTGAGATCAAATGGATTAGATGAACTTGATGGCGCTCTTGGCCTTATGAGATTAGATGCTTGCAAAAAAATGGACACTGTCTTAAATCATGCAGGAGACACTGCTGCATGTGAAAGGGAAGAAAGTGAGACTTTGGAACCCATTTCAGAGGACAATGCAATAGCCTTTAATACAAAATCTTGTCCAATATTAGACCACTCTGAAAGTGAAAGTGAAGATGATGTTGGTTTTGAAGTTCTGGATGGACTTAGACTTTCAGAATCTGTTGTCACATTCGAAGAAGTGAAATGCATGGATGACTTCAACGTTCTGGTCAATGGTTTAATCATAAATTCTGAGTTTTCGAAATACATTTTGAACAAGTATTATGAGCTTTGTTGCAGTCAAAACTCTTTCCTTCATGAACATCTTCTTGATGGTCTGAACTGTAAACTCGTAGCTGGAGTAATTTCTGAGACCGTAAACATTGCAGATGCCATCAGGGCGTGCAATATTACAACCCCAGAAGGTAATTTTTCCACCTGGGATCAGACTTTGAAAGCCTTTCAGGGTTTAGGCATGAACATCGGATTCCTTCTAGCCCGGCTAGACCAGCTTGTAAGTCTTGCTTCAAAATCAAAAAGATTTAAAGAGGCTAGACTTGAAAAGGACCAGGCAGAAGAAGAGATGAGGTGCTTGGAGGCAAAGCTTTTCGAAGTAAAAGAGGCTGTAAATAGGCTTGAATCTGAGATTGAGATGCTAAATACAAGTTCAGAGAACCCTGAGCTTGTGTTCCAAGAGGTGGCTAAATCCTCTTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

452

Amino Acids

50.53

Weight (kDa)

5.16

Isoelectric Point (pI)

51.2

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1336
AciI CCGC 1 cut(s) 440
AclI AACGTT 1 cut(s) 861
AclWI GGATC 2 cut(s) 269, 1089
AcsI RAATTY 3 cut(s) 353, 539, 886
AfiI CCNNNNNNNGG 1 cut(s) 1336
AflIII ACRYGT 1 cut(s) 316
AjnI CCWGG 2 cut(s) 1076, 1206
AjuI GAANNNNNNNTTGG 2 cut(s) 762, 794
AluBI AGCT 8 cut(s) 30, 227, 493, 928, 995, 1150, 1243, 1321
AluI AGCT 8 cut(s) 30, 227, 493, 928, 995, 1150, 1243, 1321
Alw21I GWGCWC 2 cut(s) 32, 253
Alw26I GTCTC 3 cut(s) 645, 677, 1004
AlwI GGATC 2 cut(s) 269, 1089
AlwNI CAGNNNCTG 1 cut(s) 818
AoxI GGCC 1 cut(s) 595
ApeKI GCWGC 1 cut(s) 659
ApoI RAATTY 3 cut(s) 353, 539, 886
Asp700I GAANNNNTTC 1 cut(s) 963
AspLEI GCGC 1 cut(s) 590
AspS9I GGNCC 1 cut(s) 1204
AsuC2I CCSGG 1 cut(s) 1138
AsuII TTCGAA 4 cut(s) 423, 831, 899, 1248
AvaII GGWCC 1 cut(s) 1204
BanII GRGCYC 1 cut(s) 32
BbsI GAAGAC 2 cut(s) 225, 335
Bbv12I GWGCWC 2 cut(s) 32, 253
BbvI GCAGC 1 cut(s) 646
BccI CCATC 7 cut(s) 319, 459, 528, 578, 791, 965, 1040
BcgI CGANNNNNNTGC 2 cut(s) 1102, 1136
BciT130I CCWGG 2 cut(s) 1078, 1208
BcnI CCSGG 1 cut(s) 1138
BcoDI GTCTC 3 cut(s) 645, 677, 1004
BfaI CTAG 6 cut(s) 272, 288, 524, 1133, 1142, 1191
BfoI RGCGCY 1 cut(s) 591
BglI GCCNNNNNGGC 1 cut(s) 1037
BisI GCNGC 1 cut(s) 660
BlsI GCNGC 1 cut(s) 661
Bme1390I CCNGG 3 cut(s) 1078, 1138, 1208
Bme18I GGWCC 1 cut(s) 1204
BmgT120I GGNCC 1 cut(s) 1204
BmiI GGNNCC 1 cut(s) 693
BmrFI CCNGG 3 cut(s) 1078, 1138, 1208
BmrI ACTGGG 1 cut(s) 333
BmsI GCATC 4 cut(s) 156, 601, 1018, 1281
BmuI ACTGGG 1 cut(s) 333
BpiI GAAGAC 2 cut(s) 225, 335
BpmI CTGGAG 1 cut(s) 1017
Bpu10I CCTNAGC 1 cut(s) 1317
Bpu14I TTCGAA 4 cut(s) 423, 831, 899, 1248
BpuMI CCSGG 1 cut(s) 1138
BsaI GGTCTC 1 cut(s) 1004
BsaJI CCNNGG 2 cut(s) 74, 1077
BsaWI WCCGGW 1 cut(s) 18
Bsc4I CCNNNNNNNGG 1 cut(s) 1336
Bse1I ACTGG 2 cut(s) 328, 501
Bse3DI GCAATG 1 cut(s) 1020
BseBI CCWGG 2 cut(s) 1078, 1208
BseDI CCNNGG 2 cut(s) 74, 1077
BseGI GGATG 3 cut(s) 470, 802, 856
BseLI CCNNNNNNNGG 1 cut(s) 1336
BseMI GCAATG 1 cut(s) 1020
BseMII CTCAG 7 cut(s) 134, 242, 446, 882, 999, 1272, 1308
BseNI ACTGG 2 cut(s) 328, 501
BseRI GAGGAG 1 cut(s) 22
BseXI GCAGC 1 cut(s) 646
BseYI CCCAGC 1 cut(s) 337
BshFI GGCC 1 cut(s) 597
BsiHKAI GWGCWC 2 cut(s) 32, 253
BsiSI CCGG 2 cut(s) 19, 1138
BslFI GGGAC 1 cut(s) 465
BslI CCNNNNNNNGG 1 cut(s) 1336
BsmAI GTCTC 3 cut(s) 645, 677, 1004
BsmFI GGGAC 1 cut(s) 465
BsnI GGCC 1 cut(s) 597
Bso31I GGTCTC 1 cut(s) 1004
Bsp119I TTCGAA 4 cut(s) 423, 831, 899, 1248
Bsp1286I GDGCHC 2 cut(s) 32, 253
Bsp143I GATC 3 cut(s) 274, 560, 1081
BspACI CCGC 1 cut(s) 440
BspANI GGCC 1 cut(s) 597
BspCNI CTCAG 7 cut(s) 133, 241, 447, 883, 1000, 1273, 1309
BspHI TCATGA 1 cut(s) 955
BspLI GGNNCC 1 cut(s) 693
BspPI GGATC 2 cut(s) 269, 1089
BspT104I TTCGAA 4 cut(s) 423, 831, 899, 1248
BspTNI GGTCTC 1 cut(s) 1004
BsrDI GCAATG 1 cut(s) 1020
BsrI ACTGG 2 cut(s) 328, 501
BssECI CCNNGG 2 cut(s) 74, 1077
BssMI GATC 3 cut(s) 274, 560, 1081
BssT1I CCWWGG 1 cut(s) 74
Bst2UI CCWGG 2 cut(s) 1078, 1208
Bst4CI ACNGT 4 cut(s) 199, 634, 983, 1015
Bst6I CTCTTC 1 cut(s) 1212
BstBI TTCGAA 4 cut(s) 423, 831, 899, 1248
BstC8I GCNNGC 2 cut(s) 616, 1042
BstF5I GGATG 3 cut(s) 470, 802, 856
BstH2I RGCGCY 1 cut(s) 591
BstHHI GCGC 1 cut(s) 590
BstKTI GATC 3 cut(s) 277, 563, 1084
BstMAI GTCTC 3 cut(s) 645, 677, 1004
BstMBI GATC 3 cut(s) 274, 560, 1081
BstMWI GCNNNNNNNGC 3 cut(s) 594, 1037, 1147
BstNI CCWGG 2 cut(s) 1078, 1208
BstNSI RCATGY 2 cut(s) 320, 666
BstSCI CCNGG 3 cut(s) 1076, 1136, 1206
BstV1I GCAGC 1 cut(s) 646
BstV2I GAAGAC 2 cut(s) 225, 335
BstX2I RGATCY 1 cut(s) 274
BstYI RGATCY 1 cut(s) 274
BsuRI GGCC 1 cut(s) 597
BtsCI GGATG 3 cut(s) 470, 802, 856
BtsI GCAGTG 1 cut(s) 654
BtsIMutI CAGTG 4 cut(s) 157, 204, 630, 654
Cac8I GCNNGC 2 cut(s) 616, 1042
CaiI CAGNNNCTG 1 cut(s) 818
CciI TCATGA 1 cut(s) 955
CfoI GCGC 1 cut(s) 590
Cfr13I GGNCC 1 cut(s) 1204
CviAII CATG 6 cut(s) 317, 644, 663, 847, 956, 1114
DpnI GATC 3 cut(s) 276, 562, 1083
DpnII GATC 3 cut(s) 274, 560, 1081
DraI TTTAAA 1 cut(s) 1183
Eam1104I CTCTTC 1 cut(s) 1212
EarI CTCTTC 1 cut(s) 1212
EciI GGCGGA 1 cut(s) 455
Ecl136II GAGCTC 1 cut(s) 30
Eco130I CCWWGG 1 cut(s) 74
Eco24I GRGCYC 1 cut(s) 32
Eco31I GGTCTC 1 cut(s) 1004
Eco47I GGWCC 1 cut(s) 1204
Eco53kI GAGCTC 1 cut(s) 30
EcoICRI GAGCTC 1 cut(s) 30
EcoRII CCWGG 2 cut(s) 1076, 1206
EcoT14I CCWWGG 1 cut(s) 74
EcoT22I ATGCAT 1 cut(s) 848
EcoT38I GRGCYC 1 cut(s) 32
ErhI CCWWGG 1 cut(s) 74
FaeI CATG 6 cut(s) 320, 647, 666, 850, 959, 1117
FalI AAGNNNNNCTT 6 cut(s) 291, 323, 670, 702, 1147, 1179
FaqI GGGAC 1 cut(s) 465
FatI CATG 6 cut(s) 316, 643, 662, 846, 955, 1113
Fnu4HI GCNGC 1 cut(s) 660
FokI GGATG 3 cut(s) 477, 809, 863
FriOI GRGCYC 1 cut(s) 32
Fsp4HI GCNGC 1 cut(s) 660
FspBI CTAG 6 cut(s) 272, 288, 524, 1133, 1142, 1191
GlaI GCGC 1 cut(s) 589
GluI GCNGC 1 cut(s) 660
GsaI CCCAGC 1 cut(s) 341
GsuI CTGGAG 1 cut(s) 1017
HaeII RGCGCY 1 cut(s) 591
HaeIII GGCC 1 cut(s) 597
HapII CCGG 2 cut(s) 19, 1138
HhaI GCGC 1 cut(s) 590
Hin1II CATG 6 cut(s) 320, 647, 666, 850, 959, 1117
Hin6I GCGC 1 cut(s) 588
HinP1I GCGC 1 cut(s) 588
HindIII AAGCTT 1 cut(s) 1241
HinfI GANTC 4 cut(s) 330, 815, 1125, 1277
HpaII CCGG 2 cut(s) 19, 1138
Hpy166II GTNNAC 2 cut(s) 986, 1018
Hpy188III TCNNGA 6 cut(s) 14, 242, 398, 794, 956, 968
Hpy8I GTNNAC 2 cut(s) 986, 1018
Hpy99I CGWCG 1 cut(s) 69
HpyAV CCTTC 5 cut(s) 494, 543, 962, 1055, 1139
HpyCH4III ACNGT 4 cut(s) 199, 634, 983, 1015
HpyCH4IV ACGT 1 cut(s) 861
HpyCH4V TGCA 8 cut(s) 618, 647, 662, 713, 846, 936, 1025, 1044
HpyF10VI GCNNNNNNNGC 3 cut(s) 594, 1037, 1147
HpySE526I ACGT 1 cut(s) 861
Hsp92II CATG 6 cut(s) 320, 647, 666, 850, 959, 1117
HspAI GCGC 1 cut(s) 588
Kzo9I GATC 3 cut(s) 274, 560, 1081
LmnI GCTCC 1 cut(s) 35
Lsp1109I GCAGC 1 cut(s) 646
LweI GCATC 4 cut(s) 156, 601, 1018, 1281
MaeI CTAG 6 cut(s) 272, 288, 524, 1133, 1142, 1191
MaeII ACGT 1 cut(s) 861
MaeIII GTNAC 4 cut(s) 123, 319, 526, 823
MalI GATC 3 cut(s) 276, 562, 1083
MboI GATC 3 cut(s) 274, 560, 1081
MfeI CAATTG 1 cut(s) 480
MflI RGATCY 1 cut(s) 274
MhlI GDGCHC 2 cut(s) 32, 253
MluCI AATT 6 cut(s) 353, 480, 539, 886, 1002, 1066
Mph1103I ATGCAT 1 cut(s) 848
MroXI GAANNNNTTC 1 cut(s) 963
MseI TTAA 5 cut(s) 543, 638, 723, 878, 1182
MspI CCGG 2 cut(s) 19, 1138
MspR9I CCNGG 3 cut(s) 1078, 1138, 1208
MunI CAATTG 1 cut(s) 480
MvaI CCWGG 2 cut(s) 1078, 1208
MwoI GCNNNNNNNGC 3 cut(s) 594, 1037, 1147
NciI CCSGG 1 cut(s) 1138
NdeII GATC 3 cut(s) 274, 560, 1081
NlaIII CATG 6 cut(s) 320, 647, 666, 850, 959, 1117
NlaIV GGNNCC 1 cut(s) 693
NmuCI GTSAC 3 cut(s) 123, 526, 823
NsiI ATGCAT 1 cut(s) 848
NspI RCATGY 2 cut(s) 320, 666
NspV TTCGAA 4 cut(s) 423, 831, 899, 1248
PagI TCATGA 1 cut(s) 955
PciI ACATGT 1 cut(s) 316
PdmI GAANNNNTTC 1 cut(s) 963
PfeI GAWTC 4 cut(s) 330, 815, 1125, 1277
PflFI GACNNNGTC 1 cut(s) 632
PflMI CCANNNNNTGG 1 cut(s) 1336
PkrI GCNGC 1 cut(s) 661
PscI ACATGT 1 cut(s) 316
Psp124BI GAGCTC 1 cut(s) 32
Psp1406I AACGTT 1 cut(s) 861
Psp6I CCWGG 2 cut(s) 1076, 1206
PspFI CCCAGC 1 cut(s) 337
PspGI CCWGG 2 cut(s) 1076, 1206
PspN4I GGNNCC 1 cut(s) 693
PspPI GGNCC 1 cut(s) 1204
PstNI CAGNNNCTG 1 cut(s) 818
PsuI RGATCY 1 cut(s) 274
PsyI GACNNNGTC 1 cut(s) 632
SacI GAGCTC 1 cut(s) 32
SaqAI TTAA 5 cut(s) 543, 638, 723, 878, 1182
SatI GCNGC 1 cut(s) 660
Sau3AI GATC 3 cut(s) 274, 560, 1081
Sau96I GGNCC 1 cut(s) 1204
ScrFI CCNGG 3 cut(s) 1078, 1138, 1208
SduI GDGCHC 2 cut(s) 32, 253
SfaNI GCATC 4 cut(s) 156, 601, 1018, 1281
SfuI TTCGAA 4 cut(s) 423, 831, 899, 1248
SinI GGWCC 1 cut(s) 1204
Sse9I AATT 6 cut(s) 353, 480, 539, 886, 1002, 1066
SsiI CCGC 1 cut(s) 440
SspI AATATT 2 cut(s) 744, 1048
SspMI CTAG 6 cut(s) 272, 288, 524, 1133, 1142, 1191
SstI GAGCTC 1 cut(s) 32
StyD4I CCNGG 3 cut(s) 1076, 1136, 1206
StyI CCWWGG 1 cut(s) 74
TaaI ACNGT 4 cut(s) 199, 634, 983, 1015
TaiI ACGT 1 cut(s) 864
TaqI TCGA 4 cut(s) 423, 831, 899, 1248
TasI AATT 6 cut(s) 353, 480, 539, 886, 1002, 1066
TfiI GAWTC 4 cut(s) 330, 815, 1125, 1277
Tru1I TTAA 5 cut(s) 543, 638, 723, 878, 1182
Tru9I TTAA 5 cut(s) 543, 638, 723, 878, 1182
TscAI CASTG 4 cut(s) 157, 204, 637, 661
TseFI GTSAC 3 cut(s) 123, 526, 823
TseI GCWGC 1 cut(s) 659
Tsp45I GTSAC 3 cut(s) 123, 526, 823
TspDTI ATGAA 6 cut(s) 99, 420, 591, 944, 972, 1130
TspRI CASTG 4 cut(s) 157, 204, 637, 661
Tth111I GACNNNGTC 1 cut(s) 632
Van91I CCANNNNNTGG 1 cut(s) 1336
VpaK11BI GGWCC 1 cut(s) 1204
XapI RAATTY 3 cut(s) 353, 539, 886
XceI RCATGY 2 cut(s) 320, 666
XmnI GAANNNNTTC 1 cut(s) 963
XspI CTAG 6 cut(s) 272, 288, 524, 1133, 1142, 1191
Zsp2I ATGCAT 1 cut(s) 848
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.