Prupe.8G122600_v2.0.a1

B3 domain-containing protein Os01g0234100-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0001540
Physical Location & Seq
Forward (+)
14820045 .. 14822956
2912 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G122600.1

Sequence Viewer

Length: 1251 bp
ATGAGGGAGCCAATGGCTGTTCTTCAAGATCAGAACCGGAACTTCTCTTACCCACCATCCGAGAGCATGAAAATGGAAAAGCTGTTTGGGCAAAAATCTTCATGCCTCGAACTGATGGCTTTAAGCAATGTAGTCAATGTTAATCCCATTATGTCTCAAAGTCATAAACGAAAGAGAGTCATTACAAGAGTTAAGGTAGAAAGCTTATATGATAGTTCCCAAGCTCAGTCTTCTGTTATGGAAAGAGCGAAGAAGGTTGAAGCAAGCCTAGATCCTAAGTTCCCTGTCCTGCTCAAAGTTATGATGCCATCACATGTTACTGGAGGATTCTGCCTTAGTATTCCGAAGAAATTTAGCTGTCAGCATTTGCCAAAACAAGATACGATGATTATTTTGGAAGATGAAGATGGGAAGGAATTCGAAACGAAATATCTTGTGGAGAAGGGTGGACTGAGTGGTGGATGGAGAGGGTTTTCAATTGCTCACAAGCTACTGCAGGGAGATATTGTGATTTTCCATGTAGTGGCGCCTTTTAAAATTAAGGTGTATATTGTTAGATCAAATGGTTTAGATGAAGTGGACTGCGTTCATGGCCTTATGAGGTTAGATGCTTGCACTAAACAGATTAATGCTGGACAGATTATTCCATATGAAACCGAAAAGTCTGGTCCGGTATCAGATCAGTCTGAAAATGATAGCGAAGATCTTGGTTTTGAATTTATGGATGGACTTAGACTTGCAGAGTCTGATGTCACTTTCAAAGAAGTGAAATGCATGGAGAACTTCACCGTTGTCGTCAATGGTTTAGTCATAAATTCTGAGTTTTCGAATTACATTCTGACCAAGTATTATGAGCTTTGTTGCAGTCAAAACGCATTCCTTCATGAACATCTTCTTGAGGGTCTGAACTGCAAATTGATAGCTGGAGTGATTTCTGAGACAGTCAACATTGCAGATGCCATCAGGGCCTGCAAGATTACTACCACCGAAGGTGATTTTTCCACTTGGAGTAAGACTTTGAAAGCTTTTGAGGATTTAGGCATGAATTCCGGGTTCCTGCGTGTCCGGCTGGACCGGCTTGCAAGTCTTGCTTCAGAATCAAAAAGATTGAAGGAGGCTAGACTTGAAAAGGACCATCCAGAAGAAGAGATGACGAGCTTGGATGCAAATCCTTTGGAAGTAAAAGAGACCATAAATATGACTTCAATTCTCGAACTTACATTCCAAGAAGTGGCTAAAGCCCCTTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

417

Amino Acids

46.78

Weight (kDa)

5.55

Isoelectric Point (pI)

45.16

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 526
AccB7I CCANNNNNTGG 2 cut(s) 523, 1231
AclWI GGATC 1 cut(s) 266
AcsI RAATTY 5 cut(s) 350, 416, 716, 814, 1045
AcuI CTGAAG 1 cut(s) 1077
AcyI GRCGYC 1 cut(s) 527
AfiI CCNNNNNNNGG 2 cut(s) 523, 1231
AflIII ACRYGT 1 cut(s) 313
AgsI TTSAA 9 cut(s) 26, 260, 477, 716, 760, 1021, 1111, 1127, 1206
AjuI GAANNNNNNNTTGG 2 cut(s) 69, 101
AloI GAACNNNNNNTCC 2 cut(s) 1206, 1238
AluBI AGCT 9 cut(s) 82, 204, 224, 357, 490, 856, 923, 1025, 1158
AluI AGCT 9 cut(s) 82, 204, 224, 357, 490, 856, 923, 1025, 1158
Alw26I GTCTC 3 cut(s) 159, 932, 1181
AlwI GGATC 1 cut(s) 266
AlwNI CAGNNNCTG 2 cut(s) 746, 969
AoxI GGCC 2 cut(s) 592, 966
ApoI RAATTY 5 cut(s) 350, 416, 716, 814, 1045
AseI ATTAAT 1 cut(s) 627
Asp700I GAANNNNTTC 2 cut(s) 416, 891
AspLEI GCGC 1 cut(s) 529
AspS9I GGNCC 4 cut(s) 668, 966, 1072, 1132
AsuC2I CCSGG 1 cut(s) 1051
AsuHPI GGTGA 2 cut(s) 778, 1004
AsuII TTCGAA 2 cut(s) 420, 827
AvaII GGWCC 3 cut(s) 668, 1072, 1132
BanI GGYRCC 1 cut(s) 526
BbsI GAAGAC 1 cut(s) 222
BccI CCATC 8 cut(s) 64, 109, 316, 401, 456, 719, 968, 1143
BcnI CCSGG 1 cut(s) 1051
BcoDI GTCTC 3 cut(s) 159, 932, 1181
BfaI CTAG 2 cut(s) 269, 1119
BfmI CTRYAG 1 cut(s) 494
BfoI RGCGCY 1 cut(s) 530
BglI GCCNNNNNGGC 1 cut(s) 965
BglII AGATCT 1 cut(s) 703
Bme1390I CCNGG 1 cut(s) 1051
Bme18I GGWCC 3 cut(s) 668, 1072, 1132
BmgT120I GGNCC 4 cut(s) 668, 966, 1072, 1132
BmiI GGNNCC 3 cut(s) 9, 528, 1055
BmrFI CCNGG 1 cut(s) 1051
BmsI GCATC 4 cut(s) 294, 598, 946, 1153
BpiI GAAGAC 1 cut(s) 222
BpmI CTGGAG 2 cut(s) 342, 945
Bpu14I TTCGAA 2 cut(s) 420, 827
BpuEI CTTGAG 1 cut(s) 917
BpuMI CCSGG 1 cut(s) 1051
BsaBI GATNNNNATC 1 cut(s) 1167
BsaHI GRCGYC 1 cut(s) 527
BsaI GGTCTC 1 cut(s) 1181
BsaJI CCNNGG 1 cut(s) 1244
BsaWI WCCGGW 2 cut(s) 36, 670
BsaXI ACNNNNNCTCC 2 cut(s) 492, 522
Bsc4I CCNNNNNNNGG 2 cut(s) 523, 1231
Bse118I RCCGGY 1 cut(s) 1074
Bse1I ACTGG 1 cut(s) 325
Bse3DI GCAATG 2 cut(s) 133, 948
Bse8I GATNNNNATC 1 cut(s) 1167
BseDI CCNNGG 1 cut(s) 1244
BseGI GGATG 5 cut(s) 56, 467, 730, 1135, 1168
BseJI GATNNNNATC 1 cut(s) 1167
BseLI CCNNNNNNNGG 2 cut(s) 523, 1231
BseMI GCAATG 2 cut(s) 133, 948
BseMII CTCAG 4 cut(s) 239, 443, 810, 927
BseNI ACTGG 1 cut(s) 325
BshFI GGCC 2 cut(s) 594, 968
BshNI GGYRCC 1 cut(s) 526
BsiSI CCGG 5 cut(s) 37, 671, 1050, 1066, 1075
BslI CCNNNNNNNGG 2 cut(s) 523, 1231
BsmAI GTCTC 3 cut(s) 159, 932, 1181
BsmI GAATGC 1 cut(s) 875
BsnI GGCC 2 cut(s) 594, 968
Bso31I GGTCTC 1 cut(s) 1181
Bsp119I TTCGAA 2 cut(s) 420, 827
Bsp143I GATC 5 cut(s) 28, 271, 557, 679, 703
BspANI GGCC 2 cut(s) 594, 968
BspCNI CTCAG 4 cut(s) 238, 444, 811, 928
BspHI TCATGA 1 cut(s) 883
BspLI GGNNCC 3 cut(s) 9, 528, 1055
BspMAI CTGCAG 1 cut(s) 498
BspPI GGATC 1 cut(s) 266
BspT104I TTCGAA 2 cut(s) 420, 827
BspT107I GGYRCC 1 cut(s) 526
BspTNI GGTCTC 1 cut(s) 1181
BsrDI GCAATG 2 cut(s) 133, 948
BsrFI RCCGGY 1 cut(s) 1074
BsrI ACTGG 1 cut(s) 325
BssAI RCCGGY 1 cut(s) 1074
BssECI CCNNGG 1 cut(s) 1244
BssMI GATC 5 cut(s) 28, 271, 557, 679, 703
BssNI GRCGYC 1 cut(s) 527
BssT1I CCWWGG 1 cut(s) 1244
Bst4CI ACNGT 2 cut(s) 790, 943
Bst6I CTCTTC 1 cut(s) 1140
BstACI GRCGYC 1 cut(s) 527
BstAPI GCANNNNNTGC 1 cut(s) 1088
BstBI TTCGAA 2 cut(s) 420, 827
BstC8I GCNNGC 4 cut(s) 265, 613, 970, 1080
BstDEI CTNAG 7 cut(s) 225, 276, 335, 452, 731, 819, 936
BstF5I GGATG 5 cut(s) 56, 467, 730, 1135, 1168
BstH2I RGCGCY 1 cut(s) 530
BstHHI GCGC 1 cut(s) 529
BstKTI GATC 5 cut(s) 31, 274, 560, 682, 706
BstMAI GTCTC 3 cut(s) 159, 932, 1181
BstMBI GATC 5 cut(s) 28, 271, 557, 679, 703
BstMWI GCNNNNNNNGC 6 cut(s) 88, 591, 965, 1066, 1075, 1088
BstNSI RCATGY 1 cut(s) 317
BstSCI CCNGG 1 cut(s) 1049
BstSFI CTRYAG 1 cut(s) 494
BstV2I GAAGAC 1 cut(s) 222
BstX2I RGATCY 2 cut(s) 271, 703
BstYI RGATCY 2 cut(s) 271, 703
BsuRI GGCC 2 cut(s) 594, 968
BtsCI GGATG 5 cut(s) 56, 467, 730, 1135, 1168
Cac8I GCNNGC 4 cut(s) 265, 613, 970, 1080
CaiI CAGNNNCTG 2 cut(s) 746, 969
CciI TCATGA 1 cut(s) 883
CfoI GCGC 1 cut(s) 529
Cfr10I RCCGGY 1 cut(s) 1074
Cfr13I GGNCC 4 cut(s) 668, 966, 1072, 1132
CviAII CATG 8 cut(s) 67, 102, 314, 518, 590, 775, 884, 1042
DdeI CTNAG 7 cut(s) 225, 276, 335, 452, 731, 819, 936
DinI GGCGCC 1 cut(s) 528
DpnI GATC 5 cut(s) 30, 273, 559, 681, 705
DpnII GATC 5 cut(s) 28, 271, 557, 679, 703
DraI TTTAAA 1 cut(s) 535
Eam1104I CTCTTC 1 cut(s) 1140
EarI CTCTTC 1 cut(s) 1140
Eco130I CCWWGG 1 cut(s) 1244
Eco31I GGTCTC 1 cut(s) 1181
Eco47I GGWCC 3 cut(s) 668, 1072, 1132
Eco57I CTGAAG 1 cut(s) 1077
EcoO109I RGGNCCY 1 cut(s) 966
EcoRI GAATTC 2 cut(s) 416, 1045
EcoT14I CCWWGG 1 cut(s) 1244
EcoT22I ATGCAT 1 cut(s) 776
EgeI GGCGCC 1 cut(s) 528
EheI GGCGCC 1 cut(s) 528
ErhI CCWWGG 1 cut(s) 1244
FaeI CATG 8 cut(s) 70, 105, 317, 521, 593, 778, 887, 1045
FalI AAGNNNNNCTT 2 cut(s) 1075, 1107
FatI CATG 8 cut(s) 66, 101, 313, 517, 589, 774, 883, 1041
FauNDI CATATG 1 cut(s) 649
FokI GGATG 5 cut(s) 43, 474, 737, 1122, 1175
FspBI CTAG 2 cut(s) 269, 1119
GlaI GCGC 1 cut(s) 528
GsuI CTGGAG 2 cut(s) 342, 945
HaeII RGCGCY 1 cut(s) 530
HaeIII GGCC 2 cut(s) 594, 968
HapII CCGG 5 cut(s) 37, 671, 1050, 1066, 1075
HhaI GCGC 1 cut(s) 529
Hin1I GRCGYC 1 cut(s) 527
Hin1II CATG 8 cut(s) 70, 105, 317, 521, 593, 778, 887, 1045
Hin6I GCGC 1 cut(s) 527
HinP1I GCGC 1 cut(s) 527
HincII GTYRAC 1 cut(s) 946
HindII GTYRAC 1 cut(s) 946
HindIII AAGCTT 2 cut(s) 202, 1023
HinfI GANTC 4 cut(s) 177, 327, 743, 1097
HpaII CCGG 5 cut(s) 37, 671, 1050, 1066, 1075
HphI GGTGA 2 cut(s) 778, 1004
Hpy166II GTNNAC 3 cut(s) 449, 580, 946
Hpy188III TCNNGA 5 cut(s) 26, 884, 896, 1139, 1211
Hpy8I GTNNAC 3 cut(s) 449, 580, 946
HpyAV CCTTC 6 cut(s) 247, 406, 436, 890, 983, 1105
HpyCH4III ACNGT 2 cut(s) 790, 943
HpyF10VI GCNNNNNNNGC 6 cut(s) 88, 591, 965, 1066, 1075, 1088
HpyF3I CTNAG 7 cut(s) 225, 276, 335, 452, 731, 819, 936
Hsp92I GRCGYC 1 cut(s) 527
Hsp92II CATG 8 cut(s) 70, 105, 317, 521, 593, 778, 887, 1045
HspAI GCGC 1 cut(s) 527
KasI GGCGCC 1 cut(s) 526
Kzo9I GATC 5 cut(s) 28, 271, 557, 679, 703
LmnI GCTCC 1 cut(s) 7
LweI GCATC 4 cut(s) 294, 598, 946, 1153
MaeI CTAG 2 cut(s) 269, 1119
MaeIII GTNAC 2 cut(s) 316, 751
MalI GATC 5 cut(s) 30, 273, 559, 681, 705
MboI GATC 5 cut(s) 28, 271, 557, 679, 703
MfeI CAATTG 1 cut(s) 477
MflI RGATCY 2 cut(s) 271, 703
Mly113I GGCGCC 1 cut(s) 527
MlyI GAGTC 2 cut(s) 186, 752
MnlI CCTC 7 cut(s) 116, 317, 461, 594, 892, 1024, 1108
Mph1103I ATGCAT 1 cut(s) 776
MroXI GAANNNNTTC 2 cut(s) 416, 891
MseI TTAA 6 cut(s) 122, 141, 192, 534, 540, 627
MslI CAYNNNNRTG 2 cut(s) 71, 1196
MspI CCGG 5 cut(s) 37, 671, 1050, 1066, 1075
MspR9I CCNGG 1 cut(s) 1051
MunI CAATTG 1 cut(s) 477
Mva1269I GAATGC 1 cut(s) 875
MwoI GCNNNNNNNGC 6 cut(s) 88, 591, 965, 1066, 1075, 1088
NarI GGCGCC 1 cut(s) 527
NciI CCSGG 1 cut(s) 1051
NdeI CATATG 1 cut(s) 649
NdeII GATC 5 cut(s) 28, 271, 557, 679, 703
NlaIII CATG 8 cut(s) 70, 105, 317, 521, 593, 778, 887, 1045
NlaIV GGNNCC 3 cut(s) 9, 528, 1055
NmuCI GTSAC 1 cut(s) 751
NsiI ATGCAT 1 cut(s) 776
NspI RCATGY 1 cut(s) 317
NspV TTCGAA 2 cut(s) 420, 827
PagI TCATGA 1 cut(s) 883
PciI ACATGT 1 cut(s) 313
PctI GAATGC 1 cut(s) 875
PdmI GAANNNNTTC 2 cut(s) 416, 891
PfeI GAWTC 2 cut(s) 327, 1097
PflMI CCANNNNNTGG 2 cut(s) 523, 1231
PleI GAGTC 2 cut(s) 185, 751
PluTI GGCGCC 1 cut(s) 530
PpsI GAGTC 2 cut(s) 185, 751
PscI ACATGT 1 cut(s) 313
PshBI ATTAAT 1 cut(s) 627
PspN4I GGNNCC 3 cut(s) 9, 528, 1055
PspPI GGNCC 4 cut(s) 668, 966, 1072, 1132
PsrI GAACNNNNNNTAC 2 cut(s) 32, 64
PstI CTGCAG 1 cut(s) 498
PstNI CAGNNNCTG 2 cut(s) 746, 969
PsuI RGATCY 2 cut(s) 271, 703
RseI CAYNNNNRTG 2 cut(s) 71, 1196
SaqAI TTAA 6 cut(s) 122, 141, 192, 534, 540, 627
Sau3AI GATC 5 cut(s) 28, 271, 557, 679, 703
Sau96I GGNCC 4 cut(s) 668, 966, 1072, 1132
SchI GAGTC 2 cut(s) 186, 752
ScrFI CCNGG 1 cut(s) 1051
SfaNI GCATC 4 cut(s) 294, 598, 946, 1153
SfcI CTRYAG 1 cut(s) 494
SfoI GGCGCC 1 cut(s) 528
SfuI TTCGAA 2 cut(s) 420, 827
SinI GGWCC 3 cut(s) 668, 1072, 1132
SmiMI CAYNNNNRTG 2 cut(s) 71, 1196
SmlI CTYRAG 1 cut(s) 896
SmoI CTYRAG 1 cut(s) 896
SspDI GGCGCC 1 cut(s) 526
SspMI CTAG 2 cut(s) 269, 1119
StyD4I CCNGG 1 cut(s) 1049
StyI CCWWGG 1 cut(s) 1244
TaaI ACNGT 2 cut(s) 790, 943
TaqI TCGA 4 cut(s) 108, 420, 827, 1212
TfiI GAWTC 2 cut(s) 327, 1097
Tru1I TTAA 6 cut(s) 122, 141, 192, 534, 540, 627
Tru9I TTAA 6 cut(s) 122, 141, 192, 534, 540, 627
TseFI GTSAC 1 cut(s) 751
Tsp45I GTSAC 1 cut(s) 751
TspDTI ATGAA 9 cut(s) 83, 90, 417, 578, 588, 666, 872, 900, 1058
Van91I CCANNNNNTGG 2 cut(s) 523, 1231
VpaK11BI GGWCC 3 cut(s) 668, 1072, 1132
VspI ATTAAT 1 cut(s) 627
XapI RAATTY 5 cut(s) 350, 416, 716, 814, 1045
XceI RCATGY 1 cut(s) 317
XmnI GAANNNNTTC 2 cut(s) 416, 891
XspI CTAG 2 cut(s) 269, 1119
Zsp2I ATGCAT 1 cut(s) 776
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.