pycom05g07230

B3 domain-containing protein Os01g0234100-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0001540
Physical Location & Seq
Forward (+)
10198021 .. 10200643
2623 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g07230.1

Sequence Viewer

Length: 1239 bp
ATGGCCATGGCTGTTCTTCAAGATCAGAAGCAGAAGCAGAGCTCTTCTTGCCCACCACCACCTAAGATACAGGTAGTCAGTTGCAGTAATGCTAATCCAAATATGCCTGAAAGCCGTAAGCGAAAGAGAGTTACCGTAGCAAAAAGCGTATATGAAAGAGCAAAGGAGGTTGAAGCAACCCTCGATCCTAAGTTCCCTATCCTGCTCAAAGTCATGCTGCCTTCTCACGTTGCTGGATGTTTCTGGCTTGGTCTTTCCAAGAAATTCTGCTTTGATCATATGCCAAAACAAGATAAGACAATTGTTTTGGAAGATGAAAATGGGGATAAATTTGAAACTAAATATCTTGCGCAGAAGGTGGGACTGAGTGGTGGATGGAGAGGTTTTTCAATTGCTCGCAAGCTACAGGAGGGAGATGCCGTGATTTTCCATCTGGTCACCCCGTCCAAATTTAAGGTGTATATTATTAGATCAAATGATTTAGACGGCATGGATTGCAATATTGACCCTTTGAAATTAGATGCTTCTATTAAACTGGCGGATACTGGAGATGTTATCGCATGTGAAAGGGAAGAGAGGGAGAAATTGGAAACTATTCCAAAGGATACCGCAATAGCCTTTGCTTCGATATCAGATCATTCTGGAAATGATAGTGAAGATCGTGGTTTCGAAGTTCTGGATGGACTTCAGCTTTCAGAGTCTAACGTCGCTTTCAAAGAAGTGAAAAGCATGGAGAACTTCAATGTTCTTGTCAATGGTTTAAACATAAATTCTGAGTTCTCAAAACACCTTCTGACCAAGTACTATGATCTCTGCTGCAGTCAAAACTCGTTCCTCCATGAACTTCTTCTCAAGGGTCTAAACTGCAAACTGATTTCTGGAGCAATTTCTGAGACAATCAACATTGCAGATGCCATTAGGTCCTGCAGTGTTACTACATTGGAAGGCAATTTTACCACTTGGTATAAGACTTTGAAAGCCTTTGAGGGTTTAGGCATGAATGTCGGGTTCCTGCATACCCGTCTGAACCAGCTTGTAAGTCTTGCTTCAAGATCGAAAAGATTTAAAGAGGCTAGACTTGAAAAGGACCAGGCGGAAGAAGAAATGAGGAGCTTGGAGGCAAAACTTTTGGCAGTAAAAGATGCTATAAATATGCTTGACTCTGAGATTGAGAGTCTAAACACGAGTTTGGAGAAGCTTGAGCATATGTTCCAAGAAGTGGCTAAAGCCCCTTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

413

Amino Acids

46.17

Weight (kDa)

6.14

Isoelectric Point (pI)

39.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 69 - 158 1.3e-15 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 351
AccB7I CCANNNNNTGG 1 cut(s) 1219
AciI CCGC 3 cut(s) 539, 609, 1094
AclWI GGATC 1 cut(s) 179
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 4 cut(s) 263, 329, 449, 769
AcuI CTGAAG 1 cut(s) 671
AfaI GTAC 1 cut(s) 803
AfiI CCNNNNNNNGG 1 cut(s) 1219
AjnI CCWGG 1 cut(s) 1089
AluBI AGCT 6 cut(s) 42, 403, 691, 1033, 1113, 1198
AluI AGCT 6 cut(s) 42, 403, 691, 1033, 1113, 1198
Alw21I GWGCWC 1 cut(s) 44
Alw26I GTCTC 1 cut(s) 887
AlwI GGATC 1 cut(s) 179
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 2 cut(s) 217, 816
ApoI RAATTY 4 cut(s) 263, 329, 449, 769
ArsI GACNNNNNNTTYG 2 cut(s) 289, 321
Asp700I GAANNNNTTC 2 cut(s) 594, 846
AspLEI GCGC 1 cut(s) 352
AspS9I GGNCC 2 cut(s) 921, 1087
AsuHPI GGTGA 1 cut(s) 430
AsuII TTCGAA 1 cut(s) 669
AvaII GGWCC 2 cut(s) 921, 1087
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 1 cut(s) 44
BauI CACGAG 1 cut(s) 1183
Bbv12I GWGCWC 1 cut(s) 44
BbvI GCAGC 2 cut(s) 204, 803
BccI CCATC 3 cut(s) 369, 438, 674
BceAI ACGGC 3 cut(s) 99, 404, 502
BcgI CGANNNNNNTGC 2 cut(s) 985, 1019
BciT130I CCWGG 1 cut(s) 1091
BciVI GTATCC 2 cut(s) 535, 598
BclI TGATCA 1 cut(s) 274
BcoDI GTCTC 1 cut(s) 887
BfaI CTAG 1 cut(s) 1074
BfmI CTRYAG 3 cut(s) 404, 817, 925
BfuI GTATCC 2 cut(s) 535, 598
BisI GCNGC 2 cut(s) 218, 817
BlsI GCNGC 2 cut(s) 219, 818
BmcAI AGTACT 1 cut(s) 803
Bme1390I CCNGG 1 cut(s) 1091
Bme18I GGWCC 2 cut(s) 921, 1087
BmgT120I GGNCC 2 cut(s) 921, 1087
BmiI GGNNCC 1 cut(s) 1010
BmrFI CCNGG 1 cut(s) 1091
BmsI GCATC 4 cut(s) 406, 511, 901, 1132
BpmI CTGGAG 2 cut(s) 567, 900
Bpu14I TTCGAA 1 cut(s) 669
BpuEI CTTGAG 2 cut(s) 836, 1220
BsaJI CCNNGG 2 cut(s) 6, 1232
BsaXI ACNNNNNCTCC 2 cut(s) 405, 435
Bsc4I CCNNNNNNNGG 1 cut(s) 1219
Bse1I ACTGG 2 cut(s) 540, 550
Bse3DI GCAATG 1 cut(s) 903
BseBI CCWGG 1 cut(s) 1091
BseDI CCNNGG 2 cut(s) 6, 1232
BseGI GGATG 3 cut(s) 242, 380, 685
BseLI CCNNNNNNNGG 1 cut(s) 1219
BseMI GCAATG 1 cut(s) 903
BseMII CTCAG 4 cut(s) 356, 765, 882, 1155
BseNI ACTGG 2 cut(s) 540, 550
BseRI GAGGAG 1 cut(s) 1123
BseXI GCAGC 2 cut(s) 204, 803
BshFI GGCC 1 cut(s) 5
BsiHKAI GWGCWC 1 cut(s) 44
BslFI GGGAC 1 cut(s) 375
BslI CCNNNNNNNGG 1 cut(s) 1219
BsmAI GTCTC 1 cut(s) 887
BsmFI GGGAC 1 cut(s) 375
BsnI GGCC 1 cut(s) 5
Bsp119I TTCGAA 1 cut(s) 669
Bsp1286I GDGCHC 1 cut(s) 44
Bsp143I GATC 8 cut(s) 22, 184, 274, 470, 634, 658, 808, 1052
Bsp19I CCATGG 1 cut(s) 6
BspACI CCGC 3 cut(s) 539, 609, 1094
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 4 cut(s) 357, 766, 883, 1156
BspLI GGNNCC 1 cut(s) 1010
BspMAI CTGCAG 2 cut(s) 821, 929
BspPI GGATC 1 cut(s) 179
BspQI GCTCTTC 1 cut(s) 49
BspT104I TTCGAA 1 cut(s) 669
BsrDI GCAATG 1 cut(s) 903
BsrI ACTGG 2 cut(s) 540, 550
BssECI CCNNGG 2 cut(s) 6, 1232
BssMI GATC 8 cut(s) 22, 184, 274, 470, 634, 658, 808, 1052
BssSI CACGAG 1 cut(s) 1183
BssT1I CCWWGG 2 cut(s) 6, 1232
Bst2BI CACGAG 1 cut(s) 1183
Bst2UI CCWGG 1 cut(s) 1091
Bst4CI ACNGT 1 cut(s) 136
Bst6I CTCTTC 2 cut(s) 49, 567
BstAPI GCANNNNNTGC 1 cut(s) 495
BstBI TTCGAA 1 cut(s) 669
BstC8I GCNNGC 2 cut(s) 397, 401
BstDEI CTNAG 6 cut(s) 63, 189, 365, 774, 891, 1164
BstDSI CCRYGG 1 cut(s) 6
BstEII GGTNACC 1 cut(s) 436
BstF5I GGATG 3 cut(s) 242, 380, 685
BstHHI GCGC 1 cut(s) 352
BstKTI GATC 8 cut(s) 25, 187, 277, 473, 637, 661, 811, 1055
BstMAI GTCTC 1 cut(s) 887
BstMBI GATC 8 cut(s) 22, 184, 274, 470, 634, 658, 808, 1052
BstMWI GCNNNNNNNGC 2 cut(s) 48, 495
BstNI CCWGG 1 cut(s) 1091
BstNSI RCATGY 1 cut(s) 564
BstPI GGTNACC 1 cut(s) 436
BstSCI CCNGG 1 cut(s) 1089
BstSFI CTRYAG 3 cut(s) 404, 817, 925
BstV1I GCAGC 2 cut(s) 204, 803
BsuI GTATCC 2 cut(s) 535, 598
BsuRI GGCC 1 cut(s) 5
BtgI CCRYGG 1 cut(s) 6
BtsCI GGATG 3 cut(s) 242, 380, 685
BtsI GCAGTG 1 cut(s) 934
BtsIMutI CAGTG 1 cut(s) 934
Cac8I GCNNGC 2 cut(s) 397, 401
CfoI GCGC 1 cut(s) 352
Cfr13I GGNCC 2 cut(s) 921, 1087
Csp6I GTAC 1 cut(s) 802
CviAII CATG 7 cut(s) 7, 214, 490, 561, 730, 839, 997
CviQI GTAC 1 cut(s) 802
DdeI CTNAG 6 cut(s) 63, 189, 365, 774, 891, 1164
DpnI GATC 8 cut(s) 24, 186, 276, 472, 636, 660, 810, 1054
DpnII GATC 8 cut(s) 22, 184, 274, 470, 634, 658, 808, 1052
DraI TTTAAA 2 cut(s) 762, 1066
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 49, 567
EarI CTCTTC 2 cut(s) 49, 567
EciI GGCGGA 2 cut(s) 554, 1109
Ecl136II GAGCTC 1 cut(s) 42
Eco130I CCWWGG 2 cut(s) 6, 1232
Eco24I GRGCYC 1 cut(s) 44
Eco32I GATATC 1 cut(s) 630
Eco47I GGWCC 2 cut(s) 921, 1087
Eco53kI GAGCTC 1 cut(s) 42
Eco57I CTGAAG 1 cut(s) 671
Eco91I GGTNACC 1 cut(s) 436
EcoICRI GAGCTC 1 cut(s) 42
EcoO109I RGGNCCY 1 cut(s) 921
EcoO65I GGTNACC 1 cut(s) 436
EcoRII CCWGG 1 cut(s) 1089
EcoRV GATATC 1 cut(s) 630
EcoT14I CCWWGG 2 cut(s) 6, 1232
EcoT38I GRGCYC 1 cut(s) 44
ErhI CCWWGG 2 cut(s) 6, 1232
FaeI CATG 7 cut(s) 10, 217, 493, 564, 733, 842, 1000
FalI AAGNNNNNCTT 2 cut(s) 1030, 1062
FaqI GGGAC 1 cut(s) 375
FatI CATG 7 cut(s) 6, 213, 489, 560, 729, 838, 996
FauNDI CATATG 2 cut(s) 279, 1206
FbaI TGATCA 1 cut(s) 274
Fnu4HI GCNGC 2 cut(s) 218, 817
FokI GGATG 3 cut(s) 249, 387, 692
FriOI GRGCYC 1 cut(s) 44
Fsp4HI GCNGC 2 cut(s) 218, 817
FspBI CTAG 1 cut(s) 1074
FspI TGCGCA 1 cut(s) 351
GlaI GCGC 1 cut(s) 351
GluI GCNGC 2 cut(s) 218, 817
GsuI CTGGAG 2 cut(s) 567, 900
HaeIII GGCC 1 cut(s) 5
HhaI GCGC 1 cut(s) 352
Hin1II CATG 7 cut(s) 10, 217, 493, 564, 733, 842, 1000
Hin6I GCGC 1 cut(s) 350
HinP1I GCGC 1 cut(s) 350
HindIII AAGCTT 1 cut(s) 1196
HinfI GANTC 3 cut(s) 698, 1160, 1174
HphI GGTGA 1 cut(s) 430
Hpy188I TCNGA 8 cut(s) 27, 634, 697, 775, 795, 892, 1026, 1165
Hpy188III TCNNGA 5 cut(s) 20, 642, 677, 879, 1050
Hpy99I CGWCG 1 cut(s) 710
HpyAV CCTTC 4 cut(s) 231, 349, 800, 938
HpyCH4III ACNGT 1 cut(s) 136
HpyCH4IV ACGT 2 cut(s) 228, 705
HpyCH4V TGCA 7 cut(s) 84, 498, 819, 867, 908, 927, 1015
HpyF10VI GCNNNNNNNGC 2 cut(s) 48, 495
HpyF3I CTNAG 6 cut(s) 63, 189, 365, 774, 891, 1164
HpySE526I ACGT 2 cut(s) 228, 705
Hsp92II CATG 7 cut(s) 10, 217, 493, 564, 733, 842, 1000
HspAI GCGC 1 cut(s) 350
Ksp22I TGATCA 1 cut(s) 274
Kzo9I GATC 8 cut(s) 22, 184, 274, 470, 634, 658, 808, 1052
LguI GCTCTTC 1 cut(s) 49
LmnI GCTCC 2 cut(s) 881, 1110
Lsp1109I GCAGC 2 cut(s) 204, 803
LweI GCATC 4 cut(s) 406, 511, 901, 1132
MaeI CTAG 1 cut(s) 1074
MaeII ACGT 2 cut(s) 228, 705
MaeIII GTNAC 3 cut(s) 130, 436, 931
MalI GATC 8 cut(s) 24, 186, 276, 472, 636, 660, 810, 1054
MboI GATC 8 cut(s) 22, 184, 274, 470, 634, 658, 808, 1052
MboII GAAGA 8 cut(s) 8, 36, 323, 584, 668, 839, 1109, 1112
MfeI CAATTG 2 cut(s) 300, 390
MhlI GDGCHC 1 cut(s) 44
MlsI TGGCCA 1 cut(s) 5
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 3 cut(s) 707, 1154, 1183
Mox20I TGGCCA 1 cut(s) 5
MroXI GAANNNNTTC 2 cut(s) 594, 846
MscI TGGCCA 1 cut(s) 5
MseI TTAA 4 cut(s) 453, 531, 761, 1065
Msp20I TGGCCA 1 cut(s) 5
MspR9I CCNGG 1 cut(s) 1091
MssI GTTTAAAC 1 cut(s) 762
MunI CAATTG 2 cut(s) 300, 390
MvaI CCWGG 1 cut(s) 1091
MwoI GCNNNNNNNGC 2 cut(s) 48, 495
NcoI CCATGG 1 cut(s) 6
NdeI CATATG 2 cut(s) 279, 1206
NdeII GATC 8 cut(s) 22, 184, 274, 470, 634, 658, 808, 1052
NlaIII CATG 7 cut(s) 10, 217, 493, 564, 733, 842, 1000
NlaIV GGNNCC 1 cut(s) 1010
NmuCI GTSAC 1 cut(s) 436
NsbI TGCGCA 1 cut(s) 351
NspI RCATGY 1 cut(s) 564
NspV TTCGAA 1 cut(s) 669
PciSI GCTCTTC 1 cut(s) 49
PdmI GAANNNNTTC 2 cut(s) 594, 846
PflMI CCANNNNNTGG 1 cut(s) 1219
PkrI GCNGC 2 cut(s) 219, 818
PleI GAGTC 3 cut(s) 706, 1154, 1182
PmeI GTTTAAAC 1 cut(s) 762
PpsI GAGTC 3 cut(s) 706, 1154, 1182
PpuMI RGGWCCY 1 cut(s) 921
Psp124BI GAGCTC 1 cut(s) 44
Psp5II RGGWCCY 1 cut(s) 921
Psp6I CCWGG 1 cut(s) 1089
PspEI GGTNACC 1 cut(s) 436
PspGI CCWGG 1 cut(s) 1089
PspN4I GGNNCC 1 cut(s) 1010
PspPI GGNCC 2 cut(s) 921, 1087
PspPPI RGGWCCY 1 cut(s) 921
PstI CTGCAG 2 cut(s) 821, 929
RsaI GTAC 1 cut(s) 803
RsaNI GTAC 1 cut(s) 802
SacI GAGCTC 1 cut(s) 44
SapI GCTCTTC 1 cut(s) 49
SaqAI TTAA 4 cut(s) 453, 531, 761, 1065
SatI GCNGC 2 cut(s) 218, 817
Sau3AI GATC 8 cut(s) 22, 184, 274, 470, 634, 658, 808, 1052
Sau96I GGNCC 2 cut(s) 921, 1087
ScaI AGTACT 1 cut(s) 803
SchI GAGTC 3 cut(s) 707, 1154, 1183
ScrFI CCNGG 1 cut(s) 1091
SduI GDGCHC 1 cut(s) 44
SfaNI GCATC 4 cut(s) 406, 511, 901, 1132
SfcI CTRYAG 3 cut(s) 404, 817, 925
SfuI TTCGAA 1 cut(s) 669
SinI GGWCC 2 cut(s) 921, 1087
SmlI CTYRAG 2 cut(s) 851, 1199
SmoI CTYRAG 2 cut(s) 851, 1199
SsiI CCGC 3 cut(s) 539, 609, 1094
SspI AATATT 1 cut(s) 502
SspMI CTAG 1 cut(s) 1074
SstI GAGCTC 1 cut(s) 44
StyD4I CCNGG 1 cut(s) 1089
StyI CCWWGG 2 cut(s) 6, 1232
TaaI ACNGT 1 cut(s) 136
TaiI ACGT 2 cut(s) 231, 708
TaqI TCGA 4 cut(s) 183, 626, 669, 1055
TatI WGTACW 1 cut(s) 801
Tru1I TTAA 4 cut(s) 453, 531, 761, 1065
Tru9I TTAA 4 cut(s) 453, 531, 761, 1065
TscAI CASTG 1 cut(s) 934
TseFI GTSAC 1 cut(s) 436
TseI GCWGC 2 cut(s) 217, 816
Tsp45I GTSAC 1 cut(s) 436
TspDTI ATGAA 4 cut(s) 168, 330, 855, 1013
TspRI CASTG 1 cut(s) 934
Van91I CCANNNNNTGG 1 cut(s) 1219
VpaK11BI GGWCC 2 cut(s) 921, 1087
XapI RAATTY 4 cut(s) 263, 329, 449, 769
XceI RCATGY 1 cut(s) 564
XmnI GAANNNNTTC 2 cut(s) 594, 846
XspI CTAG 1 cut(s) 1074
ZrmI AGTACT 1 cut(s) 803
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.