pycom05g07220

B3 domain-containing protein Os01g0234100-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0001540
Physical Location & Seq
Forward (+)
10195081 .. 10196946
1866 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g07220.5

Sequence Viewer

Length: 1176 bp
ATGATGTTTAAATGTCGTAAACGAAAGGCCCCTGTAAGAGTTACAGTAGAAAGCTTATACGATAGTTCCCAAGCTCAGTCTTCTGTTTTGGAAAAAGCGAAGAAGATCGAAGCAAGCCTAGATCATAAGTTTCCTTCACTACTCAAAGTTATGATGCCGTCAGAAGTTTCTGGAGGATTCTACCTTAATCTTGCTAAGAAATTTTGCAGTGAATATATGCCAAAACAAGATTCAATGATTGTTTTGGAAGATGAAAATGGGAAGGAATTTGGAACGAAGTATCTTGTGGAGAAGGGTGGACTGAGTGGTGGATGGAGAGGGTTTTCAATTACTCACAAGATAATGAAGGGAGATGTTGTGATTTTCCATCTAGTCACTCCTTTCAAATTTAAGAAAAAAAGGATAAGATCTATAACATTTTATTGTTTTGAGGCACTACTTCTGAATGCGTACGAGACACTCCATTCTTTTGCTACCTACTTGTTGCAGGTATATATCATTAGGTCAAATGAAGTGAACTGGAGTCTTCCTCTTATGAGGGTAGATCCTCCCATCAGAAAGATGGACACCGGAGGCTTTACTGCATGTGGAAAGGAAGAACGTGAGAATTTGGAACCTAATCCGCAGGAGAATGCAATAGTCTATTATACGGACTCTGGTCCAACATCAGATCAGTCTGGAAATGACGGCGAAGATGTTGGTTCCGAATTTCTGGATGGTCTTAGACTTTCAGAGTCTGTTGTCACTTTTAAAGAAGTGCACTGTTTGGACAACTTTAACGTTCAGGTCAATGGTTTAATCATAAACTCTGAATTTTCGAAATACGTCCTGACCAAGTATTATGAGCTTTGCCGCAGCCAGAACTCCTTCCTTCATGAACAACTTCTCGAGGGTCTGAACAGCAAACTGATTGCCGGAGTAATTTCTGAGACAGTCAACATTGCAGATGCCATCAGGACTCTGAAAGCCTTTAGTGATTTAGGCATGAATGTCCATTTCCTGCGTGCCCGCCTGGACCAGCTTACAAGTCTTGCTTCAAAATCAAAAAGACTAGAGGCAAATGTTTTGGAAGTGAAAGAGGCCATGAATAGTCTTGACTCTGAGATTGAGACTCCGAATACGAGTTTAGAGGAGCGGGAAATTCTGTTCCAAGAAATGGCTAAAGCCCCTTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

392

Amino Acids

44.32

Weight (kDa)

6.07

Isoelectric Point (pI)

45.35

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 478
AccB7I CCANNNNNTGG 1 cut(s) 1156
AccBSI CCGCTC 1 cut(s) 1135
AciI CCGC 4 cut(s) 623, 853, 1009, 1135
AclI AACGTT 1 cut(s) 780
AclWI GGATC 1 cut(s) 539
AcsI RAATTY 7 cut(s) 200, 266, 386, 607, 707, 812, 1140
AfaI GTAC 1 cut(s) 452
AfiI CCNNNNNNNGG 1 cut(s) 1156
AgsI TTSAA 4 cut(s) 234, 327, 385, 1038
AjnI CCWGG 1 cut(s) 1011
AjuI GAANNNNNNNTTGG 2 cut(s) 214, 246
AloI GAACNNNNNNTCC 2 cut(s) 1130, 1162
AluBI AGCT 4 cut(s) 54, 74, 847, 1021
AluI AGCT 4 cut(s) 54, 74, 847, 1021
Alw21I GWGCWC 1 cut(s) 762
Alw26I GTCTC 3 cut(s) 449, 923, 1103
Alw44I GTGCAC 1 cut(s) 758
AlwI GGATC 1 cut(s) 539
AlwNI CAGNNNCTG 1 cut(s) 737
Ama87I CYCGRG 1 cut(s) 887
AoxI GGCC 2 cut(s) 27, 1080
ApaLI GTGCAC 1 cut(s) 758
ApeKI GCWGC 1 cut(s) 855
ApoI RAATTY 7 cut(s) 200, 266, 386, 607, 707, 812, 1140
Asp700I GAANNNNTTC 2 cut(s) 866, 882
AspS9I GGNCC 3 cut(s) 28, 659, 1015
AsuII TTCGAA 1 cut(s) 818
AvaI CYCGRG 1 cut(s) 887
AvaII GGWCC 2 cut(s) 659, 1015
BaeGI GKGCMC 2 cut(s) 762, 1009
BbsI GAAGAC 2 cut(s) 72, 518
Bbv12I GWGCWC 1 cut(s) 762
BbvI GCAGC 1 cut(s) 867
BccI CCATC 6 cut(s) 306, 375, 556, 560, 710, 959
BceAI ACGGC 2 cut(s) 142, 703
BciT130I CCWGG 1 cut(s) 1013
BcoDI GTCTC 3 cut(s) 449, 923, 1103
BfaI CTAG 3 cut(s) 119, 371, 1052
BfuAI ACCTGC 1 cut(s) 478
BglII AGATCT 1 cut(s) 407
BisI GCNGC 2 cut(s) 853, 856
BlsI GCNGC 2 cut(s) 854, 857
Bme1390I CCNGG 1 cut(s) 1013
Bme18I GGWCC 2 cut(s) 659, 1015
BmeT110I CYCGRG 1 cut(s) 887
BmgT120I GGNCC 3 cut(s) 28, 659, 1015
BmiI GGNNCC 3 cut(s) 30, 615, 703
BmrFI CCNGG 1 cut(s) 1013
BmsI GCATC 2 cut(s) 144, 937
BoxI GACNNNNGTC 1 cut(s) 657
BpiI GAAGAC 2 cut(s) 72, 518
BplI GAGNNNNNCTC 2 cut(s) 514, 546
BpmI CTGGAG 2 cut(s) 192, 541
Bpu14I TTCGAA 1 cut(s) 818
BsaJI CCNNGG 1 cut(s) 1169
BsaWI WCCGGW 1 cut(s) 569
BsaXI ACNNNNNCTCC 2 cut(s) 342, 372
Bsc4I CCNNNNNNNGG 1 cut(s) 1156
Bse1I ACTGG 1 cut(s) 524
Bse3DI GCAATG 1 cut(s) 939
BseBI CCWGG 1 cut(s) 1013
BseDI CCNNGG 1 cut(s) 1169
BseGI GGATG 2 cut(s) 317, 721
BseLI CCNNNNNNNGG 1 cut(s) 1156
BseMI GCAATG 1 cut(s) 939
BseMII CTCAG 4 cut(s) 89, 293, 918, 1092
BseNI ACTGG 1 cut(s) 524
BseRI GAGGAG 1 cut(s) 1145
BseSI GKGCMC 2 cut(s) 762, 1009
BseXI GCAGC 1 cut(s) 867
BshFI GGCC 2 cut(s) 29, 1082
BsiHKAI GWGCWC 1 cut(s) 762
BsiHKCI CYCGRG 1 cut(s) 887
BsiSI CCGG 2 cut(s) 570, 915
BsiWI CGTACG 1 cut(s) 450
BslI CCNNNNNNNGG 1 cut(s) 1156
BsmAI GTCTC 3 cut(s) 449, 923, 1103
BsmI GAATGC 2 cut(s) 451, 637
BsnI GGCC 2 cut(s) 29, 1082
BsoBI CYCGRG 1 cut(s) 887
Bsp119I TTCGAA 1 cut(s) 818
Bsp1286I GDGCHC 2 cut(s) 762, 1009
Bsp143I GATC 5 cut(s) 105, 121, 407, 544, 670
BspACI CCGC 4 cut(s) 623, 853, 1009, 1135
BspANI GGCC 2 cut(s) 29, 1082
BspCNI CTCAG 4 cut(s) 88, 294, 919, 1093
BspHI TCATGA 1 cut(s) 874
BspLI GGNNCC 3 cut(s) 30, 615, 703
BspMI ACCTGC 1 cut(s) 478
BspPI GGATC 1 cut(s) 539
BspT104I TTCGAA 1 cut(s) 818
BsrBI CCGCTC 1 cut(s) 1135
BsrDI GCAATG 1 cut(s) 939
BsrI ACTGG 1 cut(s) 524
BssECI CCNNGG 1 cut(s) 1169
BssMI GATC 5 cut(s) 105, 121, 407, 544, 670
BssT1I CCWWGG 1 cut(s) 1169
Bst2UI CCWGG 1 cut(s) 1013
Bst4CI ACNGT 3 cut(s) 46, 764, 934
BstBI TTCGAA 1 cut(s) 818
BstC8I GCNNGC 3 cut(s) 115, 1005, 1009
BstDEI CTNAG 6 cut(s) 75, 195, 302, 722, 927, 1101
BstF5I GGATG 2 cut(s) 317, 721
BstKTI GATC 5 cut(s) 108, 124, 410, 547, 673
BstMAI GTCTC 3 cut(s) 449, 923, 1103
BstMBI GATC 5 cut(s) 105, 121, 407, 544, 670
BstNI CCWGG 1 cut(s) 1013
BstNSI RCATGY 1 cut(s) 588
BstPAI GACNNNNGTC 1 cut(s) 657
BstSCI CCNGG 1 cut(s) 1011
BstSLI GKGCMC 2 cut(s) 762, 1009
BstV1I GCAGC 1 cut(s) 867
BstV2I GAAGAC 2 cut(s) 72, 518
BstX2I RGATCY 2 cut(s) 407, 544
BstYI RGATCY 2 cut(s) 407, 544
BsuRI GGCC 2 cut(s) 29, 1082
BtsCI GGATG 2 cut(s) 317, 721
BtsI GCAGTG 1 cut(s) 214
BtsIMutI CAGTG 2 cut(s) 214, 760
BveI ACCTGC 1 cut(s) 478
Cac8I GCNNGC 3 cut(s) 115, 1005, 1009
CaiI CAGNNNCTG 1 cut(s) 737
CciI TCATGA 1 cut(s) 874
Cfr13I GGNCC 3 cut(s) 28, 659, 1015
Csp6I GTAC 1 cut(s) 451
CviAII CATG 4 cut(s) 585, 875, 985, 1084
CviQI GTAC 1 cut(s) 451
DdeI CTNAG 6 cut(s) 75, 195, 302, 722, 927, 1101
DpnI GATC 5 cut(s) 107, 123, 409, 546, 672
DpnII GATC 5 cut(s) 105, 121, 407, 544, 670
DraI TTTAAA 2 cut(s) 10, 751
Eco130I CCWWGG 1 cut(s) 1169
Eco47I GGWCC 2 cut(s) 659, 1015
Eco88I CYCGRG 1 cut(s) 887
EcoO109I RGGNCCY 1 cut(s) 28
EcoRII CCWGG 1 cut(s) 1011
EcoT14I CCWWGG 1 cut(s) 1169
ErhI CCWWGG 1 cut(s) 1169
FaeI CATG 4 cut(s) 588, 878, 988, 1087
FalI AAGNNNNNCTT 2 cut(s) 1018, 1050
FatI CATG 4 cut(s) 584, 874, 984, 1083
FauI CCCGC 2 cut(s) 1016, 1128
Fnu4HI GCNGC 2 cut(s) 853, 856
FokI GGATG 2 cut(s) 324, 728
Fsp4HI GCNGC 2 cut(s) 853, 856
FspBI CTAG 3 cut(s) 119, 371, 1052
GluI GCNGC 2 cut(s) 853, 856
GsuI CTGGAG 2 cut(s) 192, 541
HaeIII GGCC 2 cut(s) 29, 1082
HapII CCGG 2 cut(s) 570, 915
Hin1II CATG 4 cut(s) 588, 878, 988, 1087
HincII GTYRAC 1 cut(s) 937
HindII GTYRAC 1 cut(s) 937
HindIII AAGCTT 1 cut(s) 52
HinfI GANTC 8 cut(s) 177, 230, 523, 653, 734, 958, 1097, 1111
HpaII CCGG 2 cut(s) 570, 915
Hpy166II GTNNAC 5 cut(s) 20, 299, 517, 760, 937
Hpy188III TCNNGA 8 cut(s) 171, 678, 713, 829, 875, 887, 955, 1094
Hpy8I GTNNAC 5 cut(s) 20, 299, 517, 760, 937
HpyAV CCTTC 6 cut(s) 144, 256, 286, 340, 877, 881
HpyCH4III ACNGT 3 cut(s) 46, 764, 934
HpyCH4IV ACGT 3 cut(s) 601, 780, 825
HpyCH4V TGCA 6 cut(s) 207, 487, 584, 635, 760, 944
HpyF3I CTNAG 6 cut(s) 75, 195, 302, 722, 927, 1101
HpySE526I ACGT 3 cut(s) 601, 780, 825
Hsp92II CATG 4 cut(s) 588, 878, 988, 1087
Kzo9I GATC 5 cut(s) 105, 121, 407, 544, 670
LmnI GCTCC 1 cut(s) 1132
Lsp1109I GCAGC 1 cut(s) 867
LweI GCATC 2 cut(s) 144, 937
MaeI CTAG 3 cut(s) 119, 371, 1052
MaeII ACGT 3 cut(s) 601, 780, 825
MaeIII GTNAC 3 cut(s) 40, 373, 742
MalI GATC 5 cut(s) 107, 123, 409, 546, 672
MbiI CCGCTC 1 cut(s) 1135
MboI GATC 5 cut(s) 105, 121, 407, 544, 670
MboII GAAGA 7 cut(s) 72, 112, 115, 260, 518, 608, 704
MflI RGATCY 2 cut(s) 407, 544
MhlI GDGCHC 2 cut(s) 762, 1009
MluCI AATT 9 cut(s) 200, 266, 327, 386, 607, 707, 812, 921, 1140
MlyI GAGTC 6 cut(s) 532, 647, 743, 952, 1091, 1105
MmeI TCCRAC 1 cut(s) 686
MroXI GAANNNNTTC 2 cut(s) 866, 882
MseI TTAA 6 cut(s) 9, 186, 390, 750, 777, 797
MspI CCGG 2 cut(s) 570, 915
MspR9I CCNGG 1 cut(s) 1013
Mva1269I GAATGC 2 cut(s) 451, 637
MvaI CCWGG 1 cut(s) 1013
NdeII GATC 5 cut(s) 105, 121, 407, 544, 670
NlaIII CATG 4 cut(s) 588, 878, 988, 1087
NlaIV GGNNCC 3 cut(s) 30, 615, 703
NmuCI GTSAC 2 cut(s) 373, 742
NspI RCATGY 1 cut(s) 588
NspV TTCGAA 1 cut(s) 818
PaeR7I CTCGAG 1 cut(s) 887
PagI TCATGA 1 cut(s) 874
PctI GAATGC 2 cut(s) 451, 637
PdmI GAANNNNTTC 2 cut(s) 866, 882
PfeI GAWTC 2 cut(s) 177, 230
Pfl23II CGTACG 1 cut(s) 450
PflMI CCANNNNNTGG 1 cut(s) 1156
PkrI GCNGC 2 cut(s) 854, 857
PleI GAGTC 6 cut(s) 531, 647, 742, 952, 1091, 1105
PpsI GAGTC 6 cut(s) 531, 647, 742, 952, 1091, 1105
PshAI GACNNNNGTC 1 cut(s) 657
Psp1406I AACGTT 1 cut(s) 780
Psp6I CCWGG 1 cut(s) 1011
PspGI CCWGG 1 cut(s) 1011
PspLI CGTACG 1 cut(s) 450
PspN4I GGNNCC 3 cut(s) 30, 615, 703
PspPI GGNCC 3 cut(s) 28, 659, 1015
PstNI CAGNNNCTG 1 cut(s) 737
PsuI RGATCY 2 cut(s) 407, 544
RsaI GTAC 1 cut(s) 452
RsaNI GTAC 1 cut(s) 451
SaqAI TTAA 6 cut(s) 9, 186, 390, 750, 777, 797
SatI GCNGC 2 cut(s) 853, 856
Sau3AI GATC 5 cut(s) 105, 121, 407, 544, 670
Sau96I GGNCC 3 cut(s) 28, 659, 1015
SchI GAGTC 6 cut(s) 532, 647, 743, 952, 1091, 1105
ScrFI CCNGG 1 cut(s) 1013
SduI GDGCHC 2 cut(s) 762, 1009
SfaNI GCATC 2 cut(s) 144, 937
Sfr274I CTCGAG 1 cut(s) 887
SfuI TTCGAA 1 cut(s) 818
SinI GGWCC 2 cut(s) 659, 1015
SlaI CTCGAG 1 cut(s) 887
SmlI CTYRAG 1 cut(s) 887
SmoI CTYRAG 1 cut(s) 887
Sse9I AATT 9 cut(s) 200, 266, 327, 386, 607, 707, 812, 921, 1140
SsiI CCGC 4 cut(s) 623, 853, 1009, 1135
SspMI CTAG 3 cut(s) 119, 371, 1052
StyD4I CCNGG 1 cut(s) 1011
StyI CCWWGG 1 cut(s) 1169
TaaI ACNGT 3 cut(s) 46, 764, 934
TaiI ACGT 3 cut(s) 604, 783, 828
TaqI TCGA 3 cut(s) 108, 818, 888
TasI AATT 9 cut(s) 200, 266, 327, 386, 607, 707, 812, 921, 1140
TauI GCSGC 1 cut(s) 855
TfiI GAWTC 2 cut(s) 177, 230
Tru1I TTAA 6 cut(s) 9, 186, 390, 750, 777, 797
Tru9I TTAA 6 cut(s) 9, 186, 390, 750, 777, 797
TscAI CASTG 2 cut(s) 214, 767
TseFI GTSAC 2 cut(s) 373, 742
TseI GCWGC 1 cut(s) 855
Tsp45I GTSAC 2 cut(s) 373, 742
TspDTI ATGAA 7 cut(s) 267, 359, 525, 863, 891, 1001, 1100
TspGWI ACGGA 1 cut(s) 665
TspRI CASTG 2 cut(s) 214, 767
Van91I CCANNNNNTGG 1 cut(s) 1156
VneI GTGCAC 1 cut(s) 758
VpaK11BI GGWCC 2 cut(s) 659, 1015
XapI RAATTY 7 cut(s) 200, 266, 386, 607, 707, 812, 1140
XceI RCATGY 1 cut(s) 588
XcmI CCANNNNNNNNNTGG 1 cut(s) 559
XhoI CTCGAG 1 cut(s) 887
XmnI GAANNNNTTC 2 cut(s) 866, 882
XspI CTAG 3 cut(s) 119, 371, 1052
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.