Rh6DG079100

B3 domain-containing protein Os01g0234100-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0001540
Physical Location & Seq
Reverse (-)
9013822 .. 9017314
3493 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG079100.1

Sequence Viewer

Length: 1050 bp
ATGCACTTAACTTGGGGTTGTCAGGGTATTCCTAGGTCGTTCTGCCGTGAGCATATGCCAAAGGATGACACAATGATTGTCTTGGAAGATGAAAATGGGGAAGAATACCAAACGAAATATCTTGTGGCGAAGGAAGGATTGAGCGGTGGATGGAGAGCATTCTCAGCTGCTCACAAGTTACTGGAGGGAGATGTTCTAGTTTTCCACAAAGTCATGCCATCAAAATTGAAGGTGTATATTGTTAGATCAACTGGTTCAGATGAAGTGGATTGCTCTCTTGGTCTTATCAAATTTGATCATGGCATTAGACGAATCAAAACTAAAGTTACTGCATGTGAAAGCTCAAAAAATGGGAAACTGGAACCTATTTCAGTGGAAACTCTGCATGAAAATGTTCAGAAGGAGAACACCACAGCCTGTAATACCATGTCTGGTCCAATATCAAACCAGTATCAAATAGATAGTGAAGTTCTTTATTCTGAAGTTCTGGATGGAATAAGGCTTTCAGAGTCTGTTATCCCTTTCAAAGAAGTGAGCTGCATTGAGAACTTTAAGGTCACAGTCAGTGGTTCAATCATAAATGCTGAGATCTCCGAACACCATCTGATCAAGTACTATGAGCTCTGCCGCAGTCAAAACTCATTTCTTCATGAGCATATTCTTGAGCGTCTTAACTCCAGTCTGGTTGCTGGAATAATTTCCGAGACGGTAAATCTTTCGGATGCCATTAGAGCCTGCAAGATTACTACTTCAGAGTGTGATTTTTGGACTTGGGATAGGACTCTGCAAGCTTCTGAGATGTTAGGCATGAATGTTGGGTTCTTGCGTGCCCGGCTGGACCAGCTTGCAAGCCTTGCTTCAAAATCAAAACTGTGTAAAGAGGCTAGACTTGCAAGAGACCAAGCAGTAAAGGATATGATAACCTTGGAAGCAAAGCTTGTAAGAGCCAGAGAGGTCATAAATAGGCTCAACTTTGAGATTGAGAGTCTAAATGCAAGTTCAAAGAAGCTTGAGGCTATGTTCAAAGAAGTGGCTGAGGCCCCTTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

349

Amino Acids

39.31

Weight (kDa)

5.99

Isoelectric Point (pI)

33.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 8 - 83 1.4e-13 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 144
AciI CCGC 2 cut(s) 144, 628
AcsI RAATTY 1 cut(s) 290
AcuI CTGAAG 2 cut(s) 501, 735
AfaI GTAC 1 cut(s) 614
AgsI TTSAA 6 cut(s) 229, 526, 573, 861, 1002, 1024
AluBI AGCT 8 cut(s) 167, 342, 537, 622, 791, 844, 937, 1009
AluI AGCT 8 cut(s) 167, 342, 537, 622, 791, 844, 937, 1009
Alw21I GWGCWC 1 cut(s) 624
Alw26I GTCTC 2 cut(s) 698, 891
AlwNI CAGNNNCTG 1 cut(s) 512
AoxI GGCC 1 cut(s) 1038
ApeKI GCWGC 2 cut(s) 167, 537
ApoI RAATTY 1 cut(s) 290
Asp700I GAANNNNTTC 2 cut(s) 393, 697
AspA2I CCTAGG 1 cut(s) 32
AspS9I GGNCC 3 cut(s) 434, 838, 1039
AsuC2I CCSGG 1 cut(s) 832
AvaII GGWCC 2 cut(s) 434, 838
AvrII CCTAGG 1 cut(s) 32
BaeGI GKGCMC 1 cut(s) 832
BanII GRGCYC 1 cut(s) 624
Bbv12I GWGCWC 1 cut(s) 624
BbvCI CCTCAGC 1 cut(s) 1035
BbvI GCAGC 2 cut(s) 154, 524
BccI CCATC 4 cut(s) 144, 226, 485, 609
BceAI ACGGC 1 cut(s) 30
BclI TGATCA 2 cut(s) 295, 606
BcnI CCSGG 1 cut(s) 832
BcoDI GTCTC 2 cut(s) 698, 891
BfaI CTAG 3 cut(s) 33, 197, 885
BglII AGATCT 1 cut(s) 588
BisI GCNGC 3 cut(s) 168, 538, 628
BlnI CCTAGG 1 cut(s) 32
BlsI GCNGC 3 cut(s) 169, 539, 629
BmcAI AGTACT 1 cut(s) 614
Bme1390I CCNGG 1 cut(s) 832
Bme18I GGWCC 2 cut(s) 434, 838
BmgT120I GGNCC 3 cut(s) 434, 838, 1039
BmiI GGNNCC 2 cut(s) 363, 1041
BmrFI CCNGG 1 cut(s) 832
BmsI GCATC 1 cut(s) 712
BpmI CTGGAG 2 cut(s) 203, 661
Bpu10I CCTNAGC 1 cut(s) 1035
BpuEI CTTGAG 2 cut(s) 683, 1031
BpuMI CCSGG 1 cut(s) 832
BsaI GGTCTC 1 cut(s) 891
BsaJI CCNNGG 3 cut(s) 32, 924, 1043
Bse1I ACTGG 5 cut(s) 186, 256, 363, 448, 678
BseDI CCNNGG 3 cut(s) 32, 924, 1043
BseGI GGATG 4 cut(s) 70, 155, 496, 727
BseMII CTCAG 4 cut(s) 177, 576, 786, 1026
BseNI ACTGG 5 cut(s) 186, 256, 363, 448, 678
BseSI GKGCMC 1 cut(s) 832
BseXI GCAGC 2 cut(s) 154, 524
BshFI GGCC 1 cut(s) 1040
BsiHKAI GWGCWC 1 cut(s) 624
BsiSI CCGG 1 cut(s) 832
BsmAI GTCTC 2 cut(s) 698, 891
BsmBI CGTCTC 1 cut(s) 698
BsmI GAATGC 1 cut(s) 158
BsnI GGCC 1 cut(s) 1040
Bso31I GGTCTC 1 cut(s) 891
Bsp1286I GDGCHC 2 cut(s) 624, 832
Bsp143I GATC 4 cut(s) 245, 295, 588, 606
BspACI CCGC 2 cut(s) 144, 628
BspANI GGCC 1 cut(s) 1040
BspCNI CTCAG 4 cut(s) 176, 577, 787, 1027
BspHI TCATGA 1 cut(s) 649
BspLI GGNNCC 2 cut(s) 363, 1041
BspTNI GGTCTC 1 cut(s) 891
BsrBI CCGCTC 1 cut(s) 144
BsrI ACTGG 5 cut(s) 186, 256, 363, 448, 678
BssECI CCNNGG 3 cut(s) 32, 924, 1043
BssMI GATC 4 cut(s) 245, 295, 588, 606
BssT1I CCWWGG 3 cut(s) 32, 924, 1043
Bst4CI ACNGT 3 cut(s) 562, 709, 873
BstAPI GCANNNNNTGC 1 cut(s) 854
BstC8I GCNNGC 5 cut(s) 736, 789, 828, 846, 850
BstDEI CTNAG 4 cut(s) 163, 585, 795, 1035
BstF5I GGATG 4 cut(s) 70, 155, 496, 727
BstKTI GATC 4 cut(s) 248, 298, 591, 609
BstMAI GTCTC 2 cut(s) 698, 891
BstMBI GATC 4 cut(s) 245, 295, 588, 606
BstMWI GCNNNNNNNGC 6 cut(s) 164, 731, 832, 841, 854, 890
BstNSI RCATGY 1 cut(s) 336
BstSCI CCNGG 1 cut(s) 830
BstSLI GKGCMC 1 cut(s) 832
BstV1I GCAGC 2 cut(s) 154, 524
BstX2I RGATCY 1 cut(s) 588
BstYI RGATCY 1 cut(s) 588
BsuRI GGCC 1 cut(s) 1040
BtsCI GGATG 4 cut(s) 70, 155, 496, 727
BtsIMutI CAGTG 2 cut(s) 378, 571
Cac8I GCNNGC 5 cut(s) 736, 789, 828, 846, 850
CaiI CAGNNNCTG 1 cut(s) 512
CciI TCATGA 1 cut(s) 649
Cfr13I GGNCC 3 cut(s) 434, 838, 1039
CseI GACGC 1 cut(s) 656
Csp6I GTAC 1 cut(s) 613
CviAII CATG 7 cut(s) 214, 299, 333, 386, 427, 650, 808
CviQI GTAC 1 cut(s) 613
DdeI CTNAG 4 cut(s) 163, 585, 795, 1035
DpnI GATC 4 cut(s) 247, 297, 590, 608
DpnII GATC 4 cut(s) 245, 295, 588, 606
Ecl136II GAGCTC 1 cut(s) 622
Eco130I CCWWGG 3 cut(s) 32, 924, 1043
Eco24I GRGCYC 1 cut(s) 624
Eco31I GGTCTC 1 cut(s) 891
Eco47I GGWCC 2 cut(s) 434, 838
Eco53kI GAGCTC 1 cut(s) 622
Eco57I CTGAAG 2 cut(s) 501, 735
EcoICRI GAGCTC 1 cut(s) 622
EcoO109I RGGNCCY 1 cut(s) 1039
EcoT14I CCWWGG 3 cut(s) 32, 924, 1043
EcoT38I GRGCYC 1 cut(s) 624
ErhI CCWWGG 3 cut(s) 32, 924, 1043
Esp3I CGTCTC 1 cut(s) 698
FaeI CATG 7 cut(s) 217, 302, 336, 389, 430, 653, 811
FalI AAGNNNNNCTT 4 cut(s) 841, 873, 921, 953
FatI CATG 7 cut(s) 213, 298, 332, 385, 426, 649, 807
FauNDI CATATG 1 cut(s) 54
FbaI TGATCA 2 cut(s) 295, 606
Fnu4HI GCNGC 3 cut(s) 168, 538, 628
FokI GGATG 4 cut(s) 77, 162, 503, 734
FriOI GRGCYC 1 cut(s) 624
Fsp4HI GCNGC 3 cut(s) 168, 538, 628
FspBI CTAG 3 cut(s) 33, 197, 885
GluI GCNGC 3 cut(s) 168, 538, 628
GsuI CTGGAG 2 cut(s) 203, 661
HaeIII GGCC 1 cut(s) 1040
HapII CCGG 1 cut(s) 832
HgaI GACGC 1 cut(s) 656
Hin1II CATG 7 cut(s) 217, 302, 336, 389, 430, 653, 811
HindIII AAGCTT 3 cut(s) 789, 935, 1007
HinfI GANTC 4 cut(s) 312, 509, 781, 985
HpaII CCGG 1 cut(s) 832
Hpy188III TCNNGA 3 cut(s) 488, 650, 662
HpyAV CCTTC 4 cut(s) 124, 128, 223, 394
HpyCH4III ACNGT 3 cut(s) 562, 709, 873
HpyCH4V TGCA 9 cut(s) 4, 332, 385, 540, 738, 787, 848, 893, 995
HpyF10VI GCNNNNNNNGC 6 cut(s) 164, 731, 832, 841, 854, 890
HpyF3I CTNAG 4 cut(s) 163, 585, 795, 1035
Hsp92II CATG 7 cut(s) 217, 302, 336, 389, 430, 653, 811
Ksp22I TGATCA 2 cut(s) 295, 606
Kzo9I GATC 4 cut(s) 245, 295, 588, 606
Lsp1109I GCAGC 2 cut(s) 154, 524
LweI GCATC 1 cut(s) 712
MaeI CTAG 3 cut(s) 33, 197, 885
MaeIII GTNAC 3 cut(s) 177, 325, 556
MalI GATC 4 cut(s) 247, 297, 590, 608
MbiI CCGCTC 1 cut(s) 144
MboI GATC 4 cut(s) 245, 295, 588, 606
MboII GAAGA 3 cut(s) 98, 113, 638
MflI RGATCY 1 cut(s) 588
MhlI GDGCHC 2 cut(s) 624, 832
MluCI AATT 3 cut(s) 224, 290, 696
MlyI GAGTC 3 cut(s) 518, 775, 994
MnlI CCTC 5 cut(s) 178, 874, 946, 1006, 1030
MroXI GAANNNNTTC 2 cut(s) 393, 697
MseI TTAA 3 cut(s) 8, 552, 672
MslI CAYNNNNRTG 1 cut(s) 390
MspA1I CMGCKG 1 cut(s) 167
MspI CCGG 1 cut(s) 832
MspR9I CCNGG 1 cut(s) 832
Mva1269I GAATGC 1 cut(s) 158
MwoI GCNNNNNNNGC 6 cut(s) 164, 731, 832, 841, 854, 890
NciI CCSGG 1 cut(s) 832
NdeI CATATG 1 cut(s) 54
NdeII GATC 4 cut(s) 245, 295, 588, 606
NlaIII CATG 7 cut(s) 217, 302, 336, 389, 430, 653, 811
NlaIV GGNNCC 2 cut(s) 363, 1041
NmuCI GTSAC 1 cut(s) 556
NspI RCATGY 1 cut(s) 336
PagI TCATGA 1 cut(s) 649
PctI GAATGC 1 cut(s) 158
PdmI GAANNNNTTC 2 cut(s) 393, 697
PfeI GAWTC 1 cut(s) 312
PkrI GCNGC 3 cut(s) 169, 539, 629
PleI GAGTC 3 cut(s) 517, 775, 993
PpsI GAGTC 3 cut(s) 517, 775, 993
Psp124BI GAGCTC 1 cut(s) 624
PspN4I GGNNCC 2 cut(s) 363, 1041
PspPI GGNCC 3 cut(s) 434, 838, 1039
PstNI CAGNNNCTG 1 cut(s) 512
PsuI RGATCY 1 cut(s) 588
PvuII CAGCTG 1 cut(s) 167
RsaI GTAC 1 cut(s) 614
RsaNI GTAC 1 cut(s) 613
RseI CAYNNNNRTG 1 cut(s) 390
SacI GAGCTC 1 cut(s) 624
SaqAI TTAA 3 cut(s) 8, 552, 672
SatI GCNGC 3 cut(s) 168, 538, 628
Sau3AI GATC 4 cut(s) 245, 295, 588, 606
Sau96I GGNCC 3 cut(s) 434, 838, 1039
ScaI AGTACT 1 cut(s) 614
SchI GAGTC 3 cut(s) 518, 775, 994
ScrFI CCNGG 1 cut(s) 832
SduI GDGCHC 2 cut(s) 624, 832
SfaNI GCATC 1 cut(s) 712
SinI GGWCC 2 cut(s) 434, 838
SmiMI CAYNNNNRTG 1 cut(s) 390
SmlI CTYRAG 2 cut(s) 662, 1010
SmoI CTYRAG 2 cut(s) 662, 1010
Sse9I AATT 3 cut(s) 224, 290, 696
SsiI CCGC 2 cut(s) 144, 628
SspMI CTAG 3 cut(s) 33, 197, 885
SstI GAGCTC 1 cut(s) 624
StyD4I CCNGG 1 cut(s) 830
StyI CCWWGG 3 cut(s) 32, 924, 1043
TaaI ACNGT 3 cut(s) 562, 709, 873
TasI AATT 3 cut(s) 224, 290, 696
TatI WGTACW 1 cut(s) 612
TauI GCSGC 1 cut(s) 630
TfiI GAWTC 1 cut(s) 312
Tru1I TTAA 3 cut(s) 8, 552, 672
Tru9I TTAA 3 cut(s) 8, 552, 672
TscAI CASTG 2 cut(s) 378, 571
TseFI GTSAC 1 cut(s) 556
TseI GCWGC 2 cut(s) 167, 537
Tsp45I GTSAC 1 cut(s) 556
TspDTI ATGAA 5 cut(s) 105, 276, 402, 638, 824
TspRI CASTG 2 cut(s) 378, 571
VpaK11BI GGWCC 2 cut(s) 434, 838
XapI RAATTY 1 cut(s) 290
XceI RCATGY 1 cut(s) 336
XmaJI CCTAGG 1 cut(s) 32
XmnI GAANNNNTTC 2 cut(s) 393, 697
XspI CTAG 3 cut(s) 33, 197, 885
ZrmI AGTACT 1 cut(s) 614
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.