MD05G1079200.v1.1

B3 domain-containing protein Os01g0234100-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0001540
Physical Location & Seq
Forward (+)
16671366 .. 16674109
2744 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1079200.v1.1.491

Sequence Viewer

Length: 1038 bp
ATGCTGCCTTCTCACGTTGCTGGCTGTTTCTGGCTTGGCCTTTCCAAGAAATTCTGCTTTGATCATATGCCAAAACAAGATAAGACGATTGTTTTGGAAGATGAAAATGGGGATAAATTTGAAACTAAATATCTTGCGGAGAAGGTGGGACTGAGTGGTGGATGGAGAGGTTTTTCAATTGCTCGCAAGCTACAGGAGGGAGATGCCGTGATTTTCCATCTGGTCACCCCGTCCAAATTTAAGGTGTATATTATTAGATCCAATGATTTAGACGACATGGATTGCGCTATTGACCTTGTGAAATTAGATGCTTCTATTAAACTGGCGGATACTGGAGATGTTATAGCATGTGGAAGGGAAGAGAGGGAGAAATTGGAAACTATTCCAAAGGACTCCGCAATAGCCTTTGCTTCAAAGTCTGGTTCGATATCAGATCATTCTGGAAATGATATTGAAGATCTTGGTTTTGAAGTTCTGGATGGACTTCGGCTTTCAGAGTCTAACGTCGCTTTCAAAGAAGTGAAAAGCGTGGAGAACTTCAATGTTCTTGTCAATGGTTTAAACATAAATTCTGAGTTCTCAAAACACCTTCTGACCAAGTACTACGATCTCTGCTGCAGTCAAAACTCGTTCCTCCATGAACTTCTTATCGAGGGTCTAAACTGCAAACTGATTTCTGGAGCAATTTCTGAGACAATCAACATTGCAGATGCTATCAGGTCCTGCAGTGTTACTACATTGGGATGCAATTTTTCCGCTTGGTATAAGACTTTGAAAGCCTTTGAGGGTTTAGGCATGAATGTCGGGTTCCTGCGTACCCGTCTGGACCAGCTTGTAAGTCTTGCTTCAAGATCGAAAAGATTTAAAGAGGCTAGACTTGAAAAGGAGCAGGCGGAAGAAGAGATGAGGAGCTTGGAGGCAAAACTTTTGGTAGTAAAAGATGCTATAAATATACTTGACTCTGAGATTGAGAGTCTAAACACGAGTTTGGAGAAGCTTGAGCATATGTTCCAAGAAGTGGCTAAAGCCCCTTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

346

Amino Acids

38.51

Weight (kDa)

5.21

Isoelectric Point (pI)

36.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 1 - 87 4.9e-14 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1018
AciI CCGC 5 cut(s) 137, 326, 396, 756, 893
AclWI GGATC 1 cut(s) 252
AcsI RAATTY 4 cut(s) 50, 116, 236, 568
AfaI GTAC 2 cut(s) 602, 817
AfiI CCNNNNNNNGG 1 cut(s) 1018
AluBI AGCT 4 cut(s) 190, 832, 912, 997
AluI AGCT 4 cut(s) 190, 832, 912, 997
Alw26I GTCTC 1 cut(s) 686
AlwI GGATC 1 cut(s) 252
AlwNI CAGNNNCTG 1 cut(s) 723
AoxI GGCC 1 cut(s) 37
ApeKI GCWGC 2 cut(s) 4, 615
ApoI RAATTY 4 cut(s) 50, 116, 236, 568
ArsI GACNNNNNNTTYG 2 cut(s) 76, 108
Asp700I GAANNNNTTC 1 cut(s) 381
AspLEI GCGC 1 cut(s) 287
AspS9I GGNCC 2 cut(s) 720, 826
AsuHPI GGTGA 1 cut(s) 217
AvaII GGWCC 2 cut(s) 720, 826
BauI CACGAG 1 cut(s) 982
BbvI GCAGC 1 cut(s) 602
BccI CCATC 3 cut(s) 156, 225, 473
BceAI ACGGC 1 cut(s) 191
BcgI CGANNNNNNTGC 2 cut(s) 784, 818
BciVI GTATCC 1 cut(s) 322
BclI TGATCA 1 cut(s) 61
BcoDI GTCTC 1 cut(s) 686
BfaI CTAG 1 cut(s) 873
BfmI CTRYAG 3 cut(s) 191, 616, 724
BfuI GTATCC 1 cut(s) 322
BglII AGATCT 1 cut(s) 457
BisI GCNGC 2 cut(s) 5, 616
BlsI GCNGC 2 cut(s) 6, 617
BmcAI AGTACT 1 cut(s) 602
Bme18I GGWCC 2 cut(s) 720, 826
BmgT120I GGNCC 2 cut(s) 720, 826
BmiI GGNNCC 1 cut(s) 809
BmsI GCATC 5 cut(s) 193, 298, 700, 734, 931
BpmI CTGGAG 2 cut(s) 354, 699
BpuEI CTTGAG 1 cut(s) 1019
BsaJI CCNNGG 1 cut(s) 1031
BsaXI ACNNNNNCTCC 2 cut(s) 192, 222
Bsc4I CCNNNNNNNGG 1 cut(s) 1018
Bse1I ACTGG 2 cut(s) 327, 337
Bse3DI GCAATG 1 cut(s) 702
BseDI CCNNGG 1 cut(s) 1031
BseGI GGATG 3 cut(s) 167, 484, 749
BseLI CCNNNNNNNGG 1 cut(s) 1018
BseMI GCAATG 1 cut(s) 702
BseMII CTCAG 4 cut(s) 143, 564, 681, 954
BseNI ACTGG 2 cut(s) 327, 337
BseRI GAGGAG 1 cut(s) 922
BseXI GCAGC 1 cut(s) 602
BshFI GGCC 1 cut(s) 39
BslFI GGGAC 1 cut(s) 162
BslI CCNNNNNNNGG 1 cut(s) 1018
BsmAI GTCTC 1 cut(s) 686
BsmFI GGGAC 1 cut(s) 162
BsnI GGCC 1 cut(s) 39
Bsp143I GATC 6 cut(s) 61, 257, 433, 457, 607, 851
BspACI CCGC 5 cut(s) 137, 326, 396, 756, 893
BspANI GGCC 1 cut(s) 39
BspCNI CTCAG 4 cut(s) 144, 565, 682, 955
BspLI GGNNCC 1 cut(s) 809
BspMAI CTGCAG 2 cut(s) 620, 728
BspPI GGATC 1 cut(s) 252
BsrDI GCAATG 1 cut(s) 702
BsrI ACTGG 2 cut(s) 327, 337
BssECI CCNNGG 1 cut(s) 1031
BssMI GATC 6 cut(s) 61, 257, 433, 457, 607, 851
BssSI CACGAG 1 cut(s) 982
BssT1I CCWWGG 1 cut(s) 1031
Bst2BI CACGAG 1 cut(s) 982
Bst6I CTCTTC 2 cut(s) 354, 894
BstC8I GCNNGC 4 cut(s) 22, 184, 188, 891
BstDEI CTNAG 4 cut(s) 152, 573, 690, 963
BstEII GGTNACC 1 cut(s) 223
BstF5I GGATG 3 cut(s) 167, 484, 749
BstHHI GCGC 1 cut(s) 287
BstKTI GATC 6 cut(s) 64, 260, 436, 460, 610, 854
BstMAI GTCTC 1 cut(s) 686
BstMBI GATC 6 cut(s) 61, 257, 433, 457, 607, 851
BstNSI RCATGY 1 cut(s) 351
BstPI GGTNACC 1 cut(s) 223
BstSFI CTRYAG 3 cut(s) 191, 616, 724
BstV1I GCAGC 1 cut(s) 602
BstX2I RGATCY 2 cut(s) 257, 457
BstYI RGATCY 2 cut(s) 257, 457
BsuI GTATCC 1 cut(s) 322
BsuRI GGCC 1 cut(s) 39
BtsCI GGATG 3 cut(s) 167, 484, 749
BtsI GCAGTG 1 cut(s) 733
BtsIMutI CAGTG 1 cut(s) 733
Cac8I GCNNGC 4 cut(s) 22, 184, 188, 891
CaiI CAGNNNCTG 1 cut(s) 723
CfoI GCGC 1 cut(s) 287
Cfr13I GGNCC 2 cut(s) 720, 826
Csp6I GTAC 2 cut(s) 601, 816
CviAII CATG 4 cut(s) 277, 348, 638, 796
CviQI GTAC 2 cut(s) 601, 816
DdeI CTNAG 4 cut(s) 152, 573, 690, 963
DpnI GATC 6 cut(s) 63, 259, 435, 459, 609, 853
DpnII GATC 6 cut(s) 61, 257, 433, 457, 607, 851
DraI TTTAAA 2 cut(s) 561, 865
Eam1104I CTCTTC 2 cut(s) 354, 894
EarI CTCTTC 2 cut(s) 354, 894
EciI GGCGGA 2 cut(s) 341, 908
Eco130I CCWWGG 1 cut(s) 1031
Eco32I GATATC 1 cut(s) 429
Eco47I GGWCC 2 cut(s) 720, 826
Eco91I GGTNACC 1 cut(s) 223
EcoO109I RGGNCCY 1 cut(s) 720
EcoO65I GGTNACC 1 cut(s) 223
EcoRV GATATC 1 cut(s) 429
EcoT14I CCWWGG 1 cut(s) 1031
ErhI CCWWGG 1 cut(s) 1031
FaeI CATG 4 cut(s) 280, 351, 641, 799
FalI AAGNNNNNCTT 2 cut(s) 829, 861
FaqI GGGAC 1 cut(s) 162
FatI CATG 4 cut(s) 276, 347, 637, 795
FauNDI CATATG 2 cut(s) 66, 1005
FbaI TGATCA 1 cut(s) 61
Fnu4HI GCNGC 2 cut(s) 5, 616
FokI GGATG 3 cut(s) 174, 491, 756
Fsp4HI GCNGC 2 cut(s) 5, 616
FspBI CTAG 1 cut(s) 873
GlaI GCGC 1 cut(s) 286
GluI GCNGC 2 cut(s) 5, 616
GsuI CTGGAG 2 cut(s) 354, 699
HaeIII GGCC 1 cut(s) 39
HhaI GCGC 1 cut(s) 287
Hin1II CATG 4 cut(s) 280, 351, 641, 799
Hin6I GCGC 1 cut(s) 285
HinP1I GCGC 1 cut(s) 285
HindIII AAGCTT 1 cut(s) 995
HinfI GANTC 4 cut(s) 392, 497, 959, 973
HphI GGTGA 1 cut(s) 217
Hpy188I TCNGA 6 cut(s) 433, 496, 574, 594, 691, 964
Hpy188III TCNNGA 5 cut(s) 441, 476, 678, 824, 849
Hpy99I CGWCG 1 cut(s) 509
HpyAV CCTTC 4 cut(s) 18, 136, 348, 599
HpyCH4IV ACGT 2 cut(s) 15, 504
HpyCH4V TGCA 5 cut(s) 618, 666, 707, 726, 747
HpyF3I CTNAG 4 cut(s) 152, 573, 690, 963
HpySE526I ACGT 2 cut(s) 15, 504
Hsp92II CATG 4 cut(s) 280, 351, 641, 799
HspAI GCGC 1 cut(s) 285
Ksp22I TGATCA 1 cut(s) 61
Kzo9I GATC 6 cut(s) 61, 257, 433, 457, 607, 851
LmnI GCTCC 3 cut(s) 680, 886, 909
Lsp1109I GCAGC 1 cut(s) 602
LweI GCATC 5 cut(s) 193, 298, 700, 734, 931
MaeI CTAG 1 cut(s) 873
MaeII ACGT 2 cut(s) 15, 504
MaeIII GTNAC 2 cut(s) 223, 730
MalI GATC 6 cut(s) 63, 259, 435, 459, 609, 853
MboI GATC 6 cut(s) 61, 257, 433, 457, 607, 851
MboII GAAGA 5 cut(s) 110, 371, 467, 908, 911
MfeI CAATTG 1 cut(s) 177
MflI RGATCY 2 cut(s) 257, 457
MluCI AATT 9 cut(s) 50, 116, 177, 236, 302, 371, 568, 684, 748
MlyI GAGTC 4 cut(s) 386, 506, 953, 982
MnlI CCTC 9 cut(s) 161, 190, 357, 644, 646, 778, 862, 900, 910
MroXI GAANNNNTTC 1 cut(s) 381
MseI TTAA 4 cut(s) 240, 318, 560, 864
MslI CAYNNNNRTG 1 cut(s) 742
MssI GTTTAAAC 1 cut(s) 561
MunI CAATTG 1 cut(s) 177
NdeI CATATG 2 cut(s) 66, 1005
NdeII GATC 6 cut(s) 61, 257, 433, 457, 607, 851
NlaIII CATG 4 cut(s) 280, 351, 641, 799
NlaIV GGNNCC 1 cut(s) 809
NmuCI GTSAC 1 cut(s) 223
NspI RCATGY 1 cut(s) 351
PdmI GAANNNNTTC 1 cut(s) 381
PflMI CCANNNNNTGG 1 cut(s) 1018
PkrI GCNGC 2 cut(s) 6, 617
PleI GAGTC 4 cut(s) 386, 505, 953, 981
PmeI GTTTAAAC 1 cut(s) 561
PpsI GAGTC 4 cut(s) 386, 505, 953, 981
PpuMI RGGWCCY 1 cut(s) 720
Psp5II RGGWCCY 1 cut(s) 720
PspEI GGTNACC 1 cut(s) 223
PspN4I GGNNCC 1 cut(s) 809
PspPI GGNCC 2 cut(s) 720, 826
PspPPI RGGWCCY 1 cut(s) 720
PstI CTGCAG 2 cut(s) 620, 728
PstNI CAGNNNCTG 1 cut(s) 723
PsuI RGATCY 2 cut(s) 257, 457
RsaI GTAC 2 cut(s) 602, 817
RsaNI GTAC 2 cut(s) 601, 816
RseI CAYNNNNRTG 1 cut(s) 742
SaqAI TTAA 4 cut(s) 240, 318, 560, 864
SatI GCNGC 2 cut(s) 5, 616
Sau3AI GATC 6 cut(s) 61, 257, 433, 457, 607, 851
Sau96I GGNCC 2 cut(s) 720, 826
ScaI AGTACT 1 cut(s) 602
SchI GAGTC 4 cut(s) 386, 506, 953, 982
SfaNI GCATC 5 cut(s) 193, 298, 700, 734, 931
SfcI CTRYAG 3 cut(s) 191, 616, 724
SinI GGWCC 2 cut(s) 720, 826
SmiMI CAYNNNNRTG 1 cut(s) 742
SmlI CTYRAG 1 cut(s) 998
SmoI CTYRAG 1 cut(s) 998
Sse9I AATT 9 cut(s) 50, 116, 177, 236, 302, 371, 568, 684, 748
SsiI CCGC 5 cut(s) 137, 326, 396, 756, 893
SspMI CTAG 1 cut(s) 873
StyI CCWWGG 1 cut(s) 1031
TaiI ACGT 2 cut(s) 18, 507
TaqI TCGA 3 cut(s) 425, 651, 854
TasI AATT 9 cut(s) 50, 116, 177, 236, 302, 371, 568, 684, 748
TatI WGTACW 1 cut(s) 600
Tru1I TTAA 4 cut(s) 240, 318, 560, 864
Tru9I TTAA 4 cut(s) 240, 318, 560, 864
TscAI CASTG 1 cut(s) 733
TseFI GTSAC 1 cut(s) 223
TseI GCWGC 2 cut(s) 4, 615
Tsp45I GTSAC 1 cut(s) 223
TspDTI ATGAA 3 cut(s) 117, 654, 812
TspRI CASTG 1 cut(s) 733
Van91I CCANNNNNTGG 1 cut(s) 1018
VpaK11BI GGWCC 2 cut(s) 720, 826
XapI RAATTY 4 cut(s) 50, 116, 236, 568
XceI RCATGY 1 cut(s) 351
XmnI GAANNNNTTC 1 cut(s) 381
XspI CTAG 1 cut(s) 873
ZrmI AGTACT 1 cut(s) 602
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.