RchiOBHm_Chr6g0256821

B3 domain-containing protein Os01g0234100-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0001540
Physical Location & Seq
Forward (+)
12047496 .. 12051943
4448 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ23040

Sequence Viewer

Length: 1341 bp
ATGAGTGAGACACAGAGACTCTCAATGGCTATTGTTCCATTCCAGGAACACATACCCTCTTGTTCACCCCCCAAGTTCAACACTGAGAGGCCACTAAAGGAGAAGGAACATGTTTTCAGGGACAAGTCTTCATGTCTCAGAGAGATAACCAACGTGGTCAATGCTAATACCATGAAGTCTGAAAGTGACAAACTGAAGAGATGTAAGAAATCTACTGTAGAAAGCTTGTATGATACTTCCGAAGCTCAGTGTTCTGTTATGGAGAGAGCGAAGAAGCTTGAAGCCAATCTAGCACCGGCGTTCCCTAGAGTGGCCAAAGTTATGCGCCCGTCAAATGTCACTGGATGCTTCTGTCTTAATCTTCCTAAGAAATTTTGCGGTGATCATTTGCCAAAGCAGGATACAATGATTGTTTTAGAAGATGAAAGTGGGAGAGAATTCCAAACGAAATATCTTGTGGGAAAGGCTGGACTTAGTGCTGGATGGAGAGGATTTTCAATTGCTCACAAGTTATTGGCTGGAGATGTTGTAGTTTTCCAGCGAGTTACTCCTTCCAAATTTAAGGTGTATATTGTTCGATCAAGTGGTTTAGATGTGGCAGATTGTGCTCTTGGCCTTGTTAAATTAGATGCTGGCATCAGACAAATGGATACTGCAAACTTTACTGCATGTGAAACTGCAAAAGTGTCCATTCCTCGTGAACCTATTCAAAAGAAGAACACCATAGCCTGCAATACCTCATTGGCTCCAATGTCAGATCGCTCTGGAAATGATGGTGAAGATTTTGGTTCTGAAGTTCTAGATGGAATTAGGCTTGCAGAGTCTGTTATCCCTTTCAAAGAAGTGAAAGGCATGGAAAACTTTGATGTCATTATCAATGGTTTGGTCCTAAATTCTGAGTTTTCAAAGTACCATTTGACCAAGTACTATGAGCTCTGCTGCAGTCAAAGCTCATTCCTTCATAAGAATATTCTTGAGGGTCTTAACTTCAAACTGGTTGCTGGAATAATTTCTGAGACAATCAATATTTCAGATGCCATTAGAGCCTGCAAGATTACCACTTCAGAGTGTGATTTTTTGACTTGGGATAAGACTTTGCAAGCCTCTGAGACGTTAGGCATGAATGTTGGCTTCCTGCGTACCCGGCTAAACAAGCTTGCAAGCCTTGCTTCAGAATCAAAACGATATAGTAATCTTGATGGAGGCCGAGCAGAAGGGGAAGTAAAGCTTTTGGGGAAAAAGGAGACCACAACTTGGTTTGATTCTGAGATTGAGACTCATAATTTGTTGTCTGAGAGGCTTAAAGCTATGTTCGAAGAAGTGGCCAGGGCCCCTTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

446

Amino Acids

49.67

Weight (kDa)

8.28

Isoelectric Point (pI)

40.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 105 - 194 3.5e-15 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1254
AciI CCGC 1 cut(s) 378
AcoI YGGCCR 2 cut(s) 312, 1323
AcsI RAATTY 4 cut(s) 371, 437, 557, 892
AcuI CTGAAG 4 cut(s) 215, 813, 1047, 1155
AfaI GTAC 3 cut(s) 911, 926, 1141
AfiI CCNNNNNNNGG 2 cut(s) 310, 1254
AflIII ACRYGT 1 cut(s) 109
AgsI TTSAA 7 cut(s) 79, 281, 498, 710, 838, 906, 991
AjnI CCWGG 2 cut(s) 42, 1325
AluBI AGCT 8 cut(s) 225, 245, 277, 934, 951, 1156, 1228, 1307
AluI AGCT 8 cut(s) 225, 245, 277, 934, 951, 1156, 1228, 1307
Alw21I GWGCWC 2 cut(s) 610, 936
Alw26I GTCTC 7 cut(s) 2, 10, 140, 1010, 1103, 1238, 1268
AlwNI CAGNNNCTG 1 cut(s) 824
AoxI GGCC 6 cut(s) 89, 312, 613, 1204, 1323, 1329
ApaI GGGCCC 1 cut(s) 1333
ApeKI GCWGC 1 cut(s) 939
ApoI RAATTY 4 cut(s) 371, 437, 557, 892
ArsI GACNNNNNNTTYG 2 cut(s) 1267, 1299
Asp700I GAANNNNTTC 2 cut(s) 705, 1009
AspLEI GCGC 1 cut(s) 327
AspS9I GGNCC 3 cut(s) 886, 1329, 1330
AsuC2I CCSGG 1 cut(s) 1144
AsuHPI GGTGA 3 cut(s) 57, 392, 788
AsuII TTCGAA 1 cut(s) 1314
AvaII GGWCC 1 cut(s) 886
BaeGI GKGCMC 1 cut(s) 1333
BalI TGGCCA 2 cut(s) 314, 1325
BanII GRGCYC 2 cut(s) 936, 1333
BauI CACGAG 1 cut(s) 696
BbsI GAAGAC 1 cut(s) 120
Bbv12I GWGCWC 2 cut(s) 610, 936
BbvI GCAGC 1 cut(s) 926
BccI CCATC 4 cut(s) 477, 767, 797, 1193
BciT130I CCWGG 2 cut(s) 44, 1327
BciVI GTATCC 2 cut(s) 394, 643
BclI TGATCA 1 cut(s) 382
BcnI CCSGG 1 cut(s) 1144
BcoDI GTCTC 7 cut(s) 2, 10, 140, 1010, 1103, 1238, 1268
BfaI CTAG 3 cut(s) 290, 306, 800
BfmI CTRYAG 2 cut(s) 216, 940
BfuI GTATCC 2 cut(s) 394, 643
BisI GCNGC 1 cut(s) 940
BlsI GCNGC 1 cut(s) 941
BmcAI AGTACT 1 cut(s) 926
Bme1390I CCNGG 3 cut(s) 44, 1144, 1327
Bme18I GGWCC 1 cut(s) 886
BmgT120I GGNCC 3 cut(s) 886, 1329, 1330
BmiI GGNNCC 3 cut(s) 747, 1331, 1332
BmrFI CCNGG 3 cut(s) 44, 1144, 1327
BmsI GCATC 4 cut(s) 335, 619, 645, 1024
BpiI GAAGAC 1 cut(s) 120
BpmI CTGGAG 1 cut(s) 540
Bpu14I TTCGAA 1 cut(s) 1314
BpuEI CTTGAG 1 cut(s) 995
BpuMI CCSGG 1 cut(s) 1144
BsaI GGTCTC 1 cut(s) 1238
BsaJI CCNNGG 2 cut(s) 1326, 1334
BsaXI ACNNNNNCTCC 2 cut(s) 513, 543
Bsc4I CCNNNNNNNGG 2 cut(s) 310, 1254
Bse118I RCCGGY 1 cut(s) 295
Bse1I ACTGG 2 cut(s) 346, 999
BseBI CCWGG 2 cut(s) 44, 1327
BseDI CCNNGG 2 cut(s) 1326, 1334
BseGI GGATG 2 cut(s) 350, 488
BseLI CCNNNNNNNGG 2 cut(s) 310, 1254
BseMII CTCAG 8 cut(s) 75, 151, 260, 888, 1005, 1098, 1257, 1284
BseNI ACTGG 2 cut(s) 346, 999
BseSI GKGCMC 1 cut(s) 1333
BseXI GCAGC 1 cut(s) 926
BshFI GGCC 6 cut(s) 91, 314, 615, 1206, 1325, 1331
BsiHKAI GWGCWC 2 cut(s) 610, 936
BsiSI CCGG 2 cut(s) 296, 1144
BslFI GGGAC 1 cut(s) 134
BslI CCNNNNNNNGG 2 cut(s) 310, 1254
BsmAI GTCTC 7 cut(s) 2, 10, 140, 1010, 1103, 1238, 1268
BsmBI CGTCTC 1 cut(s) 1103
BsmFI GGGAC 1 cut(s) 134
BsnI GGCC 6 cut(s) 91, 314, 615, 1206, 1325, 1331
Bso31I GGTCTC 1 cut(s) 1238
Bsp119I TTCGAA 1 cut(s) 1314
Bsp120I GGGCCC 1 cut(s) 1329
Bsp1286I GDGCHC 3 cut(s) 610, 936, 1333
Bsp143I GATC 3 cut(s) 382, 578, 757
BspACI CCGC 1 cut(s) 378
BspANI GGCC 6 cut(s) 91, 314, 615, 1206, 1325, 1331
BspCNI CTCAG 8 cut(s) 76, 150, 259, 889, 1006, 1099, 1258, 1285
BspLI GGNNCC 3 cut(s) 747, 1331, 1332
BspMAI CTGCAG 1 cut(s) 944
BspT104I TTCGAA 1 cut(s) 1314
BspTNI GGTCTC 1 cut(s) 1238
BsrFI RCCGGY 1 cut(s) 295
BsrI ACTGG 2 cut(s) 346, 999
BssAI RCCGGY 1 cut(s) 295
BssECI CCNNGG 2 cut(s) 1326, 1334
BssMI GATC 3 cut(s) 382, 578, 757
BssSI CACGAG 1 cut(s) 696
BssT1I CCWWGG 1 cut(s) 1334
Bst2BI CACGAG 1 cut(s) 696
Bst2UI CCWGG 2 cut(s) 44, 1327
Bst4CI ACNGT 1 cut(s) 217
Bst6I CTCTTC 1 cut(s) 191
BstAPI GCANNNNNTGC 2 cut(s) 605, 1166
BstBI TTCGAA 1 cut(s) 1314
BstC8I GCNNGC 7 cut(s) 634, 730, 816, 1048, 1101, 1158, 1162
BstF5I GGATG 2 cut(s) 350, 488
BstHHI GCGC 1 cut(s) 327
BstKTI GATC 3 cut(s) 385, 581, 760
BstMAI GTCTC 7 cut(s) 2, 10, 140, 1010, 1103, 1238, 1268
BstMBI GATC 3 cut(s) 382, 578, 757
BstMWI GCNNNNNNNGC 7 cut(s) 290, 605, 948, 1043, 1144, 1153, 1166
BstNI CCWGG 2 cut(s) 44, 1327
BstNSI RCATGY 2 cut(s) 113, 672
BstSCI CCNGG 3 cut(s) 42, 1142, 1325
BstSFI CTRYAG 2 cut(s) 216, 940
BstSLI GKGCMC 1 cut(s) 1333
BstV1I GCAGC 1 cut(s) 926
BstV2I GAAGAC 1 cut(s) 120
BsuI GTATCC 2 cut(s) 394, 643
BsuRI GGCC 6 cut(s) 91, 314, 615, 1206, 1325, 1331
BtsCI GGATG 2 cut(s) 350, 488
BtsIMutI CAGTG 3 cut(s) 81, 254, 339
Cac8I GCNNGC 7 cut(s) 634, 730, 816, 1048, 1101, 1158, 1162
CaiI CAGNNNCTG 1 cut(s) 824
CfoI GCGC 1 cut(s) 327
Cfr10I RCCGGY 1 cut(s) 295
Cfr13I GGNCC 3 cut(s) 886, 1329, 1330
Csp6I GTAC 3 cut(s) 910, 925, 1140
CviAII CATG 6 cut(s) 110, 132, 172, 669, 853, 1120
CviQI GTAC 3 cut(s) 910, 925, 1140
DpnI GATC 3 cut(s) 384, 580, 759
DpnII GATC 3 cut(s) 382, 578, 757
EaeI YGGCCR 2 cut(s) 312, 1323
Eam1104I CTCTTC 1 cut(s) 191
EarI CTCTTC 1 cut(s) 191
Ecl136II GAGCTC 1 cut(s) 934
Eco130I CCWWGG 1 cut(s) 1334
Eco24I GRGCYC 2 cut(s) 936, 1333
Eco31I GGTCTC 1 cut(s) 1238
Eco47I GGWCC 1 cut(s) 886
Eco53kI GAGCTC 1 cut(s) 934
Eco57I CTGAAG 4 cut(s) 215, 813, 1047, 1155
EcoICRI GAGCTC 1 cut(s) 934
EcoO109I RGGNCCY 2 cut(s) 1329, 1330
EcoRI GAATTC 1 cut(s) 437
EcoRII CCWGG 2 cut(s) 42, 1325
EcoT14I CCWWGG 1 cut(s) 1334
EcoT38I GRGCYC 2 cut(s) 936, 1333
ErhI CCWWGG 1 cut(s) 1334
Esp3I CGTCTC 1 cut(s) 1103
FaeI CATG 6 cut(s) 113, 135, 175, 672, 856, 1123
FalI AAGNNNNNCTT 4 cut(s) 1153, 1185, 1212, 1244
FaqI GGGAC 1 cut(s) 134
FatI CATG 6 cut(s) 109, 131, 171, 668, 852, 1119
FbaI TGATCA 1 cut(s) 382
Fnu4HI GCNGC 1 cut(s) 940
FokI GGATG 2 cut(s) 357, 495
FriOI GRGCYC 2 cut(s) 936, 1333
Fsp4HI GCNGC 1 cut(s) 940
FspBI CTAG 3 cut(s) 290, 306, 800
GlaI GCGC 1 cut(s) 326
GluI GCNGC 1 cut(s) 940
GsuI CTGGAG 1 cut(s) 540
HaeIII GGCC 6 cut(s) 91, 314, 615, 1206, 1325, 1331
HapII CCGG 2 cut(s) 296, 1144
HhaI GCGC 1 cut(s) 327
Hin1II CATG 6 cut(s) 113, 135, 175, 672, 856, 1123
Hin6I GCGC 1 cut(s) 325
HinP1I GCGC 1 cut(s) 325
HindIII AAGCTT 4 cut(s) 223, 275, 1154, 1226
HinfI GANTC 5 cut(s) 18, 821, 1175, 1262, 1276
HpaII CCGG 2 cut(s) 296, 1144
HphI GGTGA 3 cut(s) 57, 392, 788
Hpy166II GTNNAC 2 cut(s) 65, 701
Hpy188III TCNNGA 5 cut(s) 698, 765, 800, 974, 1196
Hpy8I GTNNAC 2 cut(s) 65, 701
HpyAV CCTTC 4 cut(s) 97, 561, 968, 1208
HpyCH4III ACNGT 1 cut(s) 217
HpyCH4IV ACGT 2 cut(s) 153, 1112
HpyCH4V TGCA 9 cut(s) 656, 668, 680, 732, 818, 942, 1050, 1099, 1160
HpyF10VI GCNNNNNNNGC 7 cut(s) 290, 605, 948, 1043, 1144, 1153, 1166
HpySE526I ACGT 2 cut(s) 153, 1112
Hsp92II CATG 6 cut(s) 113, 135, 175, 672, 856, 1123
HspAI GCGC 1 cut(s) 325
Ksp22I TGATCA 1 cut(s) 382
Kzo9I GATC 3 cut(s) 382, 578, 757
LmnI GCTCC 1 cut(s) 751
Lsp1109I GCAGC 1 cut(s) 926
LweI GCATC 4 cut(s) 335, 619, 645, 1024
MaeI CTAG 3 cut(s) 290, 306, 800
MaeII ACGT 2 cut(s) 153, 1112
MaeIII GTNAC 3 cut(s) 185, 337, 544
MalI GATC 3 cut(s) 384, 580, 759
MboI GATC 3 cut(s) 382, 578, 757
MboII GAAGA 8 cut(s) 120, 208, 283, 353, 431, 727, 791, 1328
MfeI CAATTG 1 cut(s) 498
MhlI GDGCHC 3 cut(s) 610, 936, 1333
MlsI TGGCCA 2 cut(s) 314, 1325
MluCI AATT 9 cut(s) 371, 437, 498, 557, 623, 807, 892, 1008, 1282
MluNI TGGCCA 2 cut(s) 314, 1325
MlyI GAGTC 3 cut(s) 12, 830, 1270
MnlI CCTC 9 cut(s) 67, 81, 482, 705, 748, 970, 1114, 1196, 1290
Mox20I TGGCCA 2 cut(s) 314, 1325
MroXI GAANNNNTTC 2 cut(s) 705, 1009
MscI TGGCCA 2 cut(s) 314, 1325
MseI TTAA 5 cut(s) 357, 561, 621, 984, 1302
Msp20I TGGCCA 2 cut(s) 314, 1325
MspI CCGG 2 cut(s) 296, 1144
MspR9I CCNGG 3 cut(s) 44, 1144, 1327
MunI CAATTG 1 cut(s) 498
MvaI CCWGG 2 cut(s) 44, 1327
MwoI GCNNNNNNNGC 7 cut(s) 290, 605, 948, 1043, 1144, 1153, 1166
NciI CCSGG 1 cut(s) 1144
NdeII GATC 3 cut(s) 382, 578, 757
NlaIII CATG 6 cut(s) 113, 135, 175, 672, 856, 1123
NlaIV GGNNCC 3 cut(s) 747, 1331, 1332
NmeAIII GCCGAG 1 cut(s) 1232
NmuCI GTSAC 2 cut(s) 185, 337
NspI RCATGY 2 cut(s) 113, 672
NspV TTCGAA 1 cut(s) 1314
PciI ACATGT 1 cut(s) 109
PdmI GAANNNNTTC 2 cut(s) 705, 1009
PfeI GAWTC 2 cut(s) 1175, 1262
PflMI CCANNNNNTGG 1 cut(s) 1254
PfoI TCCNGGA 1 cut(s) 42
PkrI GCNGC 1 cut(s) 941
PleI GAGTC 3 cut(s) 12, 829, 1270
PpsI GAGTC 3 cut(s) 12, 829, 1270
PscI ACATGT 1 cut(s) 109
Psp124BI GAGCTC 1 cut(s) 936
Psp6I CCWGG 2 cut(s) 42, 1325
PspGI CCWGG 2 cut(s) 42, 1325
PspN4I GGNNCC 3 cut(s) 747, 1331, 1332
PspOMI GGGCCC 1 cut(s) 1329
PspPI GGNCC 3 cut(s) 886, 1329, 1330
PstI CTGCAG 1 cut(s) 944
PstNI CAGNNNCTG 1 cut(s) 824
RsaI GTAC 3 cut(s) 911, 926, 1141
RsaNI GTAC 3 cut(s) 910, 925, 1140
SacI GAGCTC 1 cut(s) 936
SaqAI TTAA 5 cut(s) 357, 561, 621, 984, 1302
SatI GCNGC 1 cut(s) 940
Sau3AI GATC 3 cut(s) 382, 578, 757
Sau96I GGNCC 3 cut(s) 886, 1329, 1330
ScaI AGTACT 1 cut(s) 926
SchI GAGTC 3 cut(s) 12, 830, 1270
ScrFI CCNGG 3 cut(s) 44, 1144, 1327
SduI GDGCHC 3 cut(s) 610, 936, 1333
SfaNI GCATC 4 cut(s) 335, 619, 645, 1024
SfcI CTRYAG 2 cut(s) 216, 940
SfuI TTCGAA 1 cut(s) 1314
SgrAI CRCCGGYG 1 cut(s) 295
SinI GGWCC 1 cut(s) 886
SmlI CTYRAG 1 cut(s) 974
SmoI CTYRAG 1 cut(s) 974
Sse9I AATT 9 cut(s) 371, 437, 498, 557, 623, 807, 892, 1008, 1282
SsiI CCGC 1 cut(s) 378
SspI AATATT 2 cut(s) 970, 1027
SspMI CTAG 3 cut(s) 290, 306, 800
SstI GAGCTC 1 cut(s) 936
StyD4I CCNGG 3 cut(s) 42, 1142, 1325
StyI CCWWGG 1 cut(s) 1334
TaaI ACNGT 1 cut(s) 217
TaiI ACGT 2 cut(s) 156, 1115
TaqI TCGA 2 cut(s) 577, 1314
TasI AATT 9 cut(s) 371, 437, 498, 557, 623, 807, 892, 1008, 1282
TatI WGTACW 1 cut(s) 924
TfiI GAWTC 2 cut(s) 1175, 1262
Tru1I TTAA 5 cut(s) 357, 561, 621, 984, 1302
Tru9I TTAA 5 cut(s) 357, 561, 621, 984, 1302
TscAI CASTG 3 cut(s) 88, 254, 346
TseFI GTSAC 2 cut(s) 185, 337
TseI GCWGC 1 cut(s) 939
Tsp45I GTSAC 2 cut(s) 185, 337
TspDTI ATGAA 5 cut(s) 120, 188, 438, 950, 1136
TspRI CASTG 3 cut(s) 88, 254, 346
Van91I CCANNNNNTGG 1 cut(s) 1254
VpaK11BI GGWCC 1 cut(s) 886
XapI RAATTY 4 cut(s) 371, 437, 557, 892
XbaI TCTAGA 1 cut(s) 799
XceI RCATGY 2 cut(s) 113, 672
XmnI GAANNNNTTC 2 cut(s) 705, 1009
XspI CTAG 3 cut(s) 290, 306, 800
ZrmI AGTACT 1 cut(s) 926
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.