RchiOBHm_Chr6g0256841

B3 domain-containing protein Os01g0234100-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0001540
Physical Location & Seq
Forward (+)
12060136 .. 12063550
3415 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ23041

Sequence Viewer

Length: 1356 bp
ATGGCGATTGTTCAAGAACAGAAACAGAGCTCCAAAGTGTTGCAGATTAAGAGGGGAAGATCACCAAACAAGGAAGAAAAGCTGCTGAAGAGAAAATCTTCATGTCTCAAACAGACAACCAAGAACAATATGGTCAATGCTAAATCCATCAAGTCTGAAAGTAGTCTTAAACCAAAGCGATTAAAGAGAGCGACAGAAGAAAGCGTATATGACACTTCCCAAGCTCAGCTCTCTGTTCTGGAAAGAGCAAAAGAGATTGAAGCAAGTTTAGCACCAGCGTTTCCTACACTGATCAAAGTTATGCTACCGTCACATGTTACGGGAGGATTCTGGCTTGGTCTTCCTAAGAAATTCTGTTGTGTACATTTGCCAAAACATGACACGATGATTGCTTTGGAAGATGAAAGTGGGGAAGAATACCAAACCAAATATCTTGCGGACAAGGTTGGACTAAGTGGTGGATGGAGAGGCTTTTCGATTGCTCACAAGTTAGTGGAGGGAGATGTGGTAGTTTTCCATCGTGTCACTCCTTCAAAGTTCAAGGTGTATATTGTTCGATCAAAGGGTTCAGATGAATCAGATTGCTCTCTTGGCCTTATGAACCTAGGTGTTTGCATTAAAGAAATGGATACTGGGGACATTACTACTTGTGAAGGGGCAGAATATGAGGATATTAGACCTATTTCAGTGGAAAATCCTCAAAACTATAACACCATAGCCTGCAATACTAAATCCAGTCCGATTGCTGATCTGTCTGAAAATGATACTGGAAGTCTTGGTTCTGAAGTGTTTGATGGAATTAGACTCGCAGAGTCTGTGATTCCTTTCAAAGAAGTGAGCTCCTTGGAGAACTTCAACATCATCGTCGATGGTTTCATCATAAATTCCGAGTTTCCCAAACCAGTTTTGGCGAAGTACTATGACCTTTGCTGCGGTCAAAACGCATACCTTCATGAACATCTTCTGGACGGTCTTAATTGGCAACTCATAACCGGGGCAATTTCTGAAATAACCACCATCGCAGAAGGCATAAGAACCTGCAAGGCTTCCACATTAGAATGTAATTTTCCAACTTGGAATAAGACTCTACAGGCCTTTGAGGTGTTTGGCATGAATGTTGGTTTCCTGCGTACCCGGCTAGACAAGCTTGCAAGTCTCGCTTCAGAGTCAAAAAGAGGTAAAGAGGCAAGGCATGAAAGGGAACAAGCAGAAATAGAGATGAGAACCTTGGAGGCAAAGCTGTTGGAAGTGAAAAGGACCATAAGCAGGCTGTTGGAAGTAGAGACTGAGACTCAAAATGTGAGTTCAGAAAAGCTTGAGGACATGCTCCAACAAGTGGCTAAGGCCCCTTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

451

Amino Acids

50.39

Weight (kDa)

6.32

Isoelectric Point (pI)

42.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 98 - 186 7.8e-16 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1046
AccB7I CCANNNNNTGG 1 cut(s) 1336
AciI CCGC 2 cut(s) 437, 933
AcsI RAATTY 2 cut(s) 350, 883
AcuI CTGAAG 3 cut(s) 107, 804, 1146
AfaI GTAC 3 cut(s) 363, 917, 1132
AfiI CCNNNNNNNGG 2 cut(s) 1266, 1336
AflIII ACRYGT 1 cut(s) 313
AgsI TTSAA 6 cut(s) 14, 260, 534, 541, 829, 856
AluBI AGCT 8 cut(s) 30, 82, 224, 229, 840, 1147, 1240, 1315
AluI AGCT 8 cut(s) 30, 82, 224, 229, 840, 1147, 1240, 1315
Alw21I GWGCWC 2 cut(s) 32, 842
Alw26I GTCTC 4 cut(s) 110, 1160, 1277, 1283
AlwNI CAGNNNCTG 1 cut(s) 815
AoxI GGCC 3 cut(s) 592, 1092, 1344
ApeKI GCWGC 2 cut(s) 82, 930
ApoI RAATTY 2 cut(s) 350, 883
Asp700I GAANNNNTTC 2 cut(s) 97, 960
AspA2I CCTAGG 1 cut(s) 604
AspS9I GGNCC 2 cut(s) 1257, 1345
AsuC2I CCSGG 2 cut(s) 994, 1135
AsuHPI GGTGA 1 cut(s) 54
AvaII GGWCC 1 cut(s) 1257
AvrII CCTAGG 1 cut(s) 604
BanII GRGCYC 2 cut(s) 32, 842
BbsI GAAGAC 1 cut(s) 332
Bbv12I GWGCWC 2 cut(s) 32, 842
BbvI GCAGC 2 cut(s) 69, 917
BccI CCATC 6 cut(s) 155, 456, 525, 788, 863, 1025
BciVI GTATCC 1 cut(s) 622
BclI TGATCA 1 cut(s) 291
BcnI CCSGG 2 cut(s) 994, 1135
BcoDI GTCTC 4 cut(s) 110, 1160, 1277, 1283
BfaI CTAG 2 cut(s) 605, 1139
BfmI CTRYAG 1 cut(s) 1088
BfuAI ACCTGC 1 cut(s) 1046
BfuI GTATCC 1 cut(s) 622
BisI GCNGC 2 cut(s) 83, 931
BlnI CCTAGG 1 cut(s) 604
BlpI GCTNAGC 1 cut(s) 225
BlsI GCNGC 2 cut(s) 84, 932
BmcAI AGTACT 1 cut(s) 917
Bme1390I CCNGG 2 cut(s) 994, 1135
Bme18I GGWCC 1 cut(s) 1257
BmgT120I GGNCC 2 cut(s) 1257, 1345
BmiI GGNNCC 1 cut(s) 1347
BmrFI CCNGG 2 cut(s) 994, 1135
BmrI ACTGGG 1 cut(s) 642
BmuI ACTGGG 1 cut(s) 642
BpiI GAAGAC 1 cut(s) 332
Bpu10I CCTNAGC 1 cut(s) 1341
Bpu1102I GCTNAGC 1 cut(s) 225
BpuEI CTTGAG 1 cut(s) 1337
BpuMI CCSGG 2 cut(s) 994, 1135
BsaJI CCNNGG 5 cut(s) 604, 843, 993, 1227, 1349
BsaXI ACNNNNNCTCC 2 cut(s) 492, 522
Bsc4I CCNNNNNNNGG 2 cut(s) 1266, 1336
Bse1I ACTGG 4 cut(s) 637, 735, 772, 902
BseDI CCNNGG 5 cut(s) 604, 843, 993, 1227, 1349
BseGI GGATG 1 cut(s) 467
BseLI CCNNNNNNNGG 2 cut(s) 1266, 1336
BseMII CTCAG 2 cut(s) 239, 1278
BseNI ACTGG 4 cut(s) 637, 735, 772, 902
BseXI GCAGC 2 cut(s) 69, 917
BshFI GGCC 3 cut(s) 594, 1094, 1346
BsiHKAI GWGCWC 2 cut(s) 32, 842
BsiSI CCGG 2 cut(s) 993, 1135
BslFI GGGAC 1 cut(s) 650
BslI CCNNNNNNNGG 2 cut(s) 1266, 1336
BsmAI GTCTC 4 cut(s) 110, 1160, 1277, 1283
BsmFI GGGAC 1 cut(s) 650
BsnI GGCC 3 cut(s) 594, 1094, 1346
Bsp1286I GDGCHC 2 cut(s) 32, 842
Bsp1407I TGTACA 1 cut(s) 361
Bsp143I GATC 4 cut(s) 59, 291, 557, 748
Bsp1720I GCTNAGC 1 cut(s) 225
BspACI CCGC 2 cut(s) 437, 933
BspANI GGCC 3 cut(s) 594, 1094, 1346
BspCNI CTCAG 2 cut(s) 238, 1279
BspHI TCATGA 1 cut(s) 952
BspLI GGNNCC 1 cut(s) 1347
BspMI ACCTGC 1 cut(s) 1046
BsrGI TGTACA 1 cut(s) 361
BsrI ACTGG 4 cut(s) 637, 735, 772, 902
BssECI CCNNGG 5 cut(s) 604, 843, 993, 1227, 1349
BssMI GATC 4 cut(s) 59, 291, 557, 748
BssT1I CCWWGG 4 cut(s) 604, 843, 1227, 1349
Bst4CI ACNGT 2 cut(s) 309, 971
Bst6I CTCTTC 1 cut(s) 83
BstAUI TGTACA 1 cut(s) 361
BstC8I GCNNGC 3 cut(s) 721, 1149, 1268
BstDEI CTNAG 5 cut(s) 225, 345, 452, 1287, 1341
BstF5I GGATG 1 cut(s) 467
BstKTI GATC 4 cut(s) 62, 294, 560, 751
BstMAI GTCTC 4 cut(s) 110, 1160, 1277, 1283
BstMBI GATC 4 cut(s) 59, 291, 557, 748
BstMWI GCNNNNNNNGC 5 cut(s) 269, 591, 1135, 1144, 1157
BstNSI RCATGY 2 cut(s) 317, 1327
BstSCI CCNGG 2 cut(s) 992, 1133
BstSFI CTRYAG 1 cut(s) 1088
BstV1I GCAGC 2 cut(s) 69, 917
BstV2I GAAGAC 1 cut(s) 332
BsuI GTATCC 1 cut(s) 622
BsuRI GGCC 3 cut(s) 594, 1094, 1346
BtgZI GCGATG 1 cut(s) 1003
BtsCI GGATG 1 cut(s) 467
BtsIMutI CAGTG 2 cut(s) 287, 693
BveI ACCTGC 1 cut(s) 1046
Cac8I GCNNGC 3 cut(s) 721, 1149, 1268
CaiI CAGNNNCTG 1 cut(s) 815
CciI TCATGA 1 cut(s) 952
Cfr13I GGNCC 2 cut(s) 1257, 1345
Csp6I GTAC 3 cut(s) 362, 916, 1131
CviAII CATG 7 cut(s) 102, 314, 377, 953, 1111, 1193, 1324
CviQI GTAC 3 cut(s) 362, 916, 1131
DdeI CTNAG 5 cut(s) 225, 345, 452, 1287, 1341
DpnI GATC 4 cut(s) 61, 293, 559, 750
DpnII GATC 4 cut(s) 59, 291, 557, 748
Eam1104I CTCTTC 1 cut(s) 83
EarI CTCTTC 1 cut(s) 83
Ecl136II GAGCTC 2 cut(s) 30, 840
Eco130I CCWWGG 4 cut(s) 604, 843, 1227, 1349
Eco147I AGGCCT 1 cut(s) 1094
Eco24I GRGCYC 2 cut(s) 32, 842
Eco47I GGWCC 1 cut(s) 1257
Eco53kI GAGCTC 2 cut(s) 30, 840
Eco57I CTGAAG 3 cut(s) 107, 804, 1146
EcoICRI GAGCTC 2 cut(s) 30, 840
EcoO109I RGGNCCY 1 cut(s) 1345
EcoT14I CCWWGG 4 cut(s) 604, 843, 1227, 1349
EcoT38I GRGCYC 2 cut(s) 32, 842
ErhI CCWWGG 4 cut(s) 604, 843, 1227, 1349
FaeI CATG 7 cut(s) 105, 317, 380, 956, 1114, 1196, 1327
FalI AAGNNNNNCTT 2 cut(s) 1144, 1176
FaqI GGGAC 1 cut(s) 650
FatI CATG 7 cut(s) 101, 313, 376, 952, 1110, 1192, 1323
FbaI TGATCA 1 cut(s) 291
Fnu4HI GCNGC 2 cut(s) 83, 931
FokI GGATG 1 cut(s) 474
FriOI GRGCYC 2 cut(s) 32, 842
Fsp4HI GCNGC 2 cut(s) 83, 931
FspBI CTAG 2 cut(s) 605, 1139
GluI GCNGC 2 cut(s) 83, 931
HaeIII GGCC 3 cut(s) 594, 1094, 1346
HapII CCGG 2 cut(s) 993, 1135
Hin1II CATG 7 cut(s) 105, 317, 380, 956, 1114, 1196, 1327
HindIII AAGCTT 2 cut(s) 1145, 1313
HinfI GANTC 8 cut(s) 327, 575, 804, 812, 820, 1084, 1166, 1291
HpaII CCGG 2 cut(s) 993, 1135
HphI GGTGA 1 cut(s) 54
Hpy166II GTNNAC 1 cut(s) 362
Hpy188III TCNNGA 4 cut(s) 14, 239, 953, 965
Hpy8I GTNNAC 1 cut(s) 362
Hpy99I CGWCG 1 cut(s) 869
HpyAV CCTTC 4 cut(s) 540, 647, 959, 1019
HpyCH4III ACNGT 2 cut(s) 309, 971
HpyCH4V TGCA 5 cut(s) 43, 615, 723, 1041, 1151
HpyF10VI GCNNNNNNNGC 5 cut(s) 269, 591, 1135, 1144, 1157
HpyF3I CTNAG 5 cut(s) 225, 345, 452, 1287, 1341
Hsp92II CATG 7 cut(s) 105, 317, 380, 956, 1114, 1196, 1327
Ksp22I TGATCA 1 cut(s) 291
Kzo9I GATC 4 cut(s) 59, 291, 557, 748
LmnI GCTCC 3 cut(s) 35, 845, 1332
Lsp1109I GCAGC 2 cut(s) 69, 917
MaeI CTAG 2 cut(s) 605, 1139
MaeIII GTNAC 3 cut(s) 309, 316, 523
MalI GATC 4 cut(s) 61, 293, 559, 750
MboI GATC 4 cut(s) 59, 291, 557, 748
MboII GAAGA 9 cut(s) 69, 86, 90, 100, 209, 332, 410, 425, 953
MhlI GDGCHC 2 cut(s) 32, 842
MluCI AATT 6 cut(s) 350, 798, 883, 976, 999, 1063
MlyI GAGTC 5 cut(s) 798, 821, 1078, 1175, 1285
MmeI TCCRAC 5 cut(s) 427, 1094, 1224, 1254, 1354
MroXI GAANNNNTTC 2 cut(s) 97, 960
MseI TTAA 5 cut(s) 48, 168, 182, 618, 975
MslI CAYNNNNRTG 1 cut(s) 1057
MspI CCGG 2 cut(s) 993, 1135
MspR9I CCNGG 2 cut(s) 994, 1135
MwoI GCNNNNNNNGC 5 cut(s) 269, 591, 1135, 1144, 1157
NciI CCSGG 2 cut(s) 994, 1135
NdeII GATC 4 cut(s) 59, 291, 557, 748
NlaIII CATG 7 cut(s) 105, 317, 380, 956, 1114, 1196, 1327
NlaIV GGNNCC 1 cut(s) 1347
NmuCI GTSAC 2 cut(s) 309, 523
NspI RCATGY 2 cut(s) 317, 1327
PagI TCATGA 1 cut(s) 952
PceI AGGCCT 1 cut(s) 1094
PciI ACATGT 1 cut(s) 313
PdmI GAANNNNTTC 2 cut(s) 97, 960
PfeI GAWTC 3 cut(s) 327, 575, 820
PflMI CCANNNNNTGG 1 cut(s) 1336
PkrI GCNGC 2 cut(s) 84, 932
PleI GAGTC 5 cut(s) 798, 820, 1078, 1174, 1285
PpsI GAGTC 5 cut(s) 798, 820, 1078, 1174, 1285
PscI ACATGT 1 cut(s) 313
Psp124BI GAGCTC 2 cut(s) 32, 842
PspN4I GGNNCC 1 cut(s) 1347
PspPI GGNCC 2 cut(s) 1257, 1345
PstNI CAGNNNCTG 1 cut(s) 815
RsaI GTAC 3 cut(s) 363, 917, 1132
RsaNI GTAC 3 cut(s) 362, 916, 1131
RseI CAYNNNNRTG 1 cut(s) 1057
SacI GAGCTC 2 cut(s) 32, 842
SaqAI TTAA 5 cut(s) 48, 168, 182, 618, 975
SatI GCNGC 2 cut(s) 83, 931
Sau3AI GATC 4 cut(s) 59, 291, 557, 748
Sau96I GGNCC 2 cut(s) 1257, 1345
ScaI AGTACT 1 cut(s) 917
SchI GAGTC 5 cut(s) 798, 821, 1078, 1175, 1285
ScrFI CCNGG 2 cut(s) 994, 1135
SduI GDGCHC 2 cut(s) 32, 842
SfcI CTRYAG 1 cut(s) 1088
SinI GGWCC 1 cut(s) 1257
SmiMI CAYNNNNRTG 1 cut(s) 1057
SmlI CTYRAG 1 cut(s) 1316
SmoI CTYRAG 1 cut(s) 1316
Sse9I AATT 6 cut(s) 350, 798, 883, 976, 999, 1063
SseBI AGGCCT 1 cut(s) 1094
SsiI CCGC 2 cut(s) 437, 933
SspMI CTAG 2 cut(s) 605, 1139
SstI GAGCTC 2 cut(s) 32, 842
StuI AGGCCT 1 cut(s) 1094
StyD4I CCNGG 2 cut(s) 992, 1133
StyI CCWWGG 4 cut(s) 604, 843, 1227, 1349
TaaI ACNGT 2 cut(s) 309, 971
TaqI TCGA 3 cut(s) 476, 556, 867
TasI AATT 6 cut(s) 350, 798, 883, 976, 999, 1063
TatI WGTACW 2 cut(s) 361, 915
TfiI GAWTC 3 cut(s) 327, 575, 820
Tru1I TTAA 5 cut(s) 48, 168, 182, 618, 975
Tru9I TTAA 5 cut(s) 48, 168, 182, 618, 975
TscAI CASTG 2 cut(s) 294, 693
TseFI GTSAC 2 cut(s) 309, 523
TseI GCWGC 2 cut(s) 82, 930
Tsp45I GTSAC 2 cut(s) 309, 523
TspDTI ATGAA 9 cut(s) 90, 417, 588, 614, 865, 941, 969, 1127, 1209
TspRI CASTG 2 cut(s) 294, 693
Van91I CCANNNNNTGG 1 cut(s) 1336
VpaK11BI GGWCC 1 cut(s) 1257
XapI RAATTY 2 cut(s) 350, 883
XceI RCATGY 2 cut(s) 317, 1327
XcmI CCANNNNNNNNNTGG 2 cut(s) 127, 904
XmaJI CCTAGG 1 cut(s) 604
XmnI GAANNNNTTC 2 cut(s) 97, 960
XspI CTAG 2 cut(s) 605, 1139
ZrmI AGTACT 1 cut(s) 917
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.