RLG00000014649

B3 domain-containing protein Os01g0234100-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0001540
Physical Location & Seq
Forward (+)
55761649 .. 55772772
11124 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000014649

Sequence Viewer

Length: 1803 bp
ATGCCATTAGAGCTTGCAGGCTTTCCAGTTTCCACCCCCCACGGTGCTTATGTATTGTGCGAGAAGACTTTAGATGCCTTTGAGATTTTGGGTATGAATGTTGGATTCTTACGTGCCAGGCTGAAGCGGCTTCTGAGCCTTTCTTTCAGGACCAAACAGGCTCTGATAAGGAAATCAAAGGAGGCTAAACTTGAGCAAGCTCGTGCAAAGGACAAGGTGATAACTCTTGAATCCAAGTATTGGAAATGGAAAGAGAGGATGGTAAGTGCTGATGCTAAGATAAAGGCTCTAAAGGAAGTTGCAGAGAGGCGTGAACTTTTCTTCAGGAAGAGGCTGTTGCTCCATGGGGACGAGAAGAATTCCGGCTCCCATTTTAGGCAAGTTTCTAGTCCCCTTGCGTGTCTCACTTGCAATCTTTCTCTATTTTACACTACACTATTCAAGCTCAGAGTCCTAAGAGAACCCATGGCTATTGTAAAAGAACAGAAACAGAGCTCCAAGGGTTTTGCTTTGCAGAATAAGAGGGCAAGATCATCAAACAATGAAGATGAGCACACGATTGGAACCAGTGTGAGAGAATTACGCTCTGTTTTTTATTACTTTAGCAAACAAAAGAAATTTAAGAGAGCCACAGAAGAAAGTTTATATGAGACTTCTGAAGCTCAGTTTTCTGTTCTCGAAAGAGCAAATGAGGTTGCAGCAAACCTAGATCCTCAGTTTCCTAGCATGGTCAAAGTTATGTTGCCATCACATGTCTCAGGAGGATTCTGGCTTGGTATTCCTAGGTCGTTCTGCCGTGAGCATATGCCAAAGGATGACACAATGATTGTCTTGGAAGATGAAAATGGGGAAGAATACCAAACGAAATATCTTGTGGCGAAGGAAGGATTGAGCGGTGGATGGAGAGCATTCTCAGTTGCTCACAAGTTACTGGAGGGAGATGTTCTAGTTTTCCACAAAGTCATGCCATCAAAATTGAAGGTGTATATTGTTAGATCAAATGGTTCAGATGAAGTGGATTGCTCTCTTGGTCTTATCAAATTTGATCATGGCATTAAACGAATCAAAACTACAGAAGTTAGTGCATGTGAAAGCTCAAAAAATGGGAAACTGGAACCTATTTCAGTGGAAACTCTGCATGAAAATGTTCAGAAGGAGAACACCACAGCCTGTAATACCAAGTCTGGTCCAATATCAAACCAGTATCAAATAGATAGTGAAGTTCTTTATTCTGAAGTTCTGGATGGAATAAGGGTTTCAGAGTCTGTTATCCCTTTCAAAGAAGTGAGCTGCATTGAGAACTTTAAGGTCACAGTCCGTGGTTCAATCATAAATGCTGAGATCTCCGAACACCATCTGATCAAGTACTATGAGCTCTGCCGCAGTCAAAACTCATTTCTTCATGAGCATATTCTTGAGCGTCTTAACTCCAGTCTGGTTGCTGGAATAATTTCTGAGACAGTAAATCTTTCGGATGCCATTAGAGCCTGCAAGATTACTACTTCAGATTGTGATTTTTCGACTTGGGATAGGACCCTGCAAGCTTCTGAGATGTTAGGCATGAATGTTGGGTTCTTGCGTGCCCGGCTGGACCGGCTTGCAAGCCTTGCTTCAAAATCAAAACTGTGTAAAGAGGCTAGACTTGCAAGAGACCAAGCAGTAAAGGATATGATAACCTTGGAAGCAAAGCTTGTAAGAGCCAGAGAGGTCATAAATAGGCTCAACTTTGAGATTGAGAGTCTAAATACAAGTTCAAAGAAGCTTGAGGCTATGTTCAAAGAAGTGGCTAAGGCCCCTTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

601

Amino Acids

68.11

Weight (kDa)

9.1

Isoelectric Point (pI)

37.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 244 - 333 1.9e-17 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 240
AccBSI CCGCTC 1 cut(s) 894
AciI CCGC 3 cut(s) 127, 894, 1381
AclWI GGATC 1 cut(s) 704
AcsI RAATTY 3 cut(s) 358, 617, 1040
AcuI CTGAAG 5 cut(s) 143, 307, 678, 1254, 1488
AfaI GTAC 1 cut(s) 1367
AfiI CCNNNNNNNGG 2 cut(s) 240, 375
AflIII ACRYGT 1 cut(s) 751
AgsI TTSAA 8 cut(s) 230, 442, 979, 1279, 1326, 1614, 1755, 1777
AjnI CCWGG 1 cut(s) 116
Alw21I GWGCWC 3 cut(s) 497, 555, 1377
Alw26I GTCTC 5 cut(s) 407, 644, 760, 1451, 1644
AlwI GGATC 1 cut(s) 704
AlwNI CAGNNNCTG 2 cut(s) 163, 1265
AoxI GGCC 1 cut(s) 1791
ApeKI GCWGC 2 cut(s) 698, 1290
ApoI RAATTY 3 cut(s) 358, 617, 1040
Asp700I GAANNNNTTC 2 cut(s) 1146, 1450
AspA2I CCTAGG 1 cut(s) 782
AspS9I GGNCC 5 cut(s) 150, 1187, 1533, 1591, 1792
AsuC2I CCSGG 1 cut(s) 1585
AsuHPI GGTGA 1 cut(s) 229
AvaII GGWCC 4 cut(s) 150, 1187, 1533, 1591
AvrII CCTAGG 1 cut(s) 782
BaeGI GKGCMC 1 cut(s) 1585
BanII GRGCYC 2 cut(s) 497, 1377
BauI CACGAG 1 cut(s) 201
BbsI GAAGAC 1 cut(s) 71
Bbv12I GWGCWC 3 cut(s) 497, 555, 1377
BbvI GCAGC 2 cut(s) 710, 1277
BccI CCATC 6 cut(s) 253, 754, 894, 976, 1238, 1362
BceAI ACGGC 1 cut(s) 780
BciT130I CCWGG 1 cut(s) 118
BclI TGATCA 2 cut(s) 1045, 1359
BcnI CCSGG 1 cut(s) 1585
BcoDI GTCTC 5 cut(s) 407, 644, 760, 1451, 1644
BfaI CTAG 6 cut(s) 387, 707, 723, 783, 947, 1638
BfmI CTRYAG 1 cut(s) 1071
BglII AGATCT 1 cut(s) 1341
BisI GCNGC 4 cut(s) 128, 699, 1291, 1381
BlnI CCTAGG 1 cut(s) 782
BlsI GCNGC 4 cut(s) 129, 700, 1292, 1382
BmcAI AGTACT 1 cut(s) 1367
Bme1390I CCNGG 2 cut(s) 118, 1585
Bme18I GGWCC 4 cut(s) 150, 1187, 1533, 1591
BmgT120I GGNCC 5 cut(s) 150, 1187, 1533, 1591, 1792
BmiI GGNNCC 5 cut(s) 367, 565, 1116, 1535, 1794
BmrFI CCNGG 2 cut(s) 118, 1585
BmsI GCATC 3 cut(s) 64, 262, 1465
BpiI GAAGAC 1 cut(s) 71
BpmI CTGGAG 2 cut(s) 953, 1414
Bpu10I CCTNAGC 1 cut(s) 1788
BpuEI CTTGAG 3 cut(s) 212, 1436, 1784
BpuMI CCSGG 1 cut(s) 1585
BsaAI YACGTR 1 cut(s) 113
BsaI GGTCTC 1 cut(s) 1644
BsaJI CCNNGG 8 cut(s) 40, 343, 465, 498, 782, 1318, 1677, 1796
Bsc4I CCNNNNNNNGG 2 cut(s) 240, 375
Bse118I RCCGGY 1 cut(s) 1593
Bse1I ACTGG 6 cut(s) 26, 567, 936, 1116, 1201, 1431
BseBI CCWGG 1 cut(s) 118
BseDI CCNNGG 8 cut(s) 40, 343, 465, 498, 782, 1318, 1677, 1796
BseGI GGATG 5 cut(s) 264, 820, 905, 1249, 1480
BseLI CCNNNNNNNGG 2 cut(s) 240, 375
BseMII CTCAG 9 cut(s) 125, 460, 677, 728, 771, 927, 1329, 1446, 1539
BseNI ACTGG 6 cut(s) 26, 567, 936, 1116, 1201, 1431
BseSI GKGCMC 1 cut(s) 1585
BseXI GCAGC 2 cut(s) 710, 1277
BshFI GGCC 1 cut(s) 1793
BsiHKAI GWGCWC 3 cut(s) 497, 555, 1377
BsiSI CCGG 3 cut(s) 363, 1585, 1594
BslFI GGGAC 2 cut(s) 362, 375
BslI CCNNNNNNNGG 2 cut(s) 240, 375
BsmAI GTCTC 5 cut(s) 407, 644, 760, 1451, 1644
BsmFI GGGAC 2 cut(s) 362, 375
BsmI GAATGC 1 cut(s) 908
BsnI GGCC 1 cut(s) 1793
Bso31I GGTCTC 1 cut(s) 1644
Bsp1286I GDGCHC 4 cut(s) 497, 555, 1377, 1585
Bsp143I GATC 6 cut(s) 530, 709, 995, 1045, 1341, 1359
Bsp19I CCATGG 2 cut(s) 343, 465
BspACI CCGC 3 cut(s) 127, 894, 1381
BspANI GGCC 1 cut(s) 1793
BspCNI CTCAG 9 cut(s) 126, 459, 676, 727, 770, 926, 1330, 1447, 1540
BspHI TCATGA 1 cut(s) 1402
BspLI GGNNCC 5 cut(s) 367, 565, 1116, 1535, 1794
BspPI GGATC 1 cut(s) 704
BspTNI GGTCTC 1 cut(s) 1644
BsrBI CCGCTC 1 cut(s) 894
BsrFI RCCGGY 1 cut(s) 1593
BsrI ACTGG 6 cut(s) 26, 567, 936, 1116, 1201, 1431
BssAI RCCGGY 1 cut(s) 1593
BssECI CCNNGG 8 cut(s) 40, 343, 465, 498, 782, 1318, 1677, 1796
BssMI GATC 6 cut(s) 530, 709, 995, 1045, 1341, 1359
BssSI CACGAG 1 cut(s) 201
BssT1I CCWWGG 6 cut(s) 343, 465, 498, 782, 1677, 1796
Bst2BI CACGAG 1 cut(s) 201
Bst2UI CCWGG 1 cut(s) 118
Bst4CI ACNGT 4 cut(s) 44, 1315, 1462, 1626
Bst6I CTCTTC 1 cut(s) 323
BstAPI GCANNNNNTGC 1 cut(s) 1607
BstBAI YACGTR 1 cut(s) 113
BstC8I GCNNGC 8 cut(s) 15, 19, 198, 1489, 1542, 1581, 1599, 1603
BstDSI CCRYGG 4 cut(s) 40, 343, 465, 1318
BstF5I GGATG 5 cut(s) 264, 820, 905, 1249, 1480
BstKTI GATC 6 cut(s) 533, 712, 998, 1048, 1344, 1362
BstMAI GTCTC 5 cut(s) 407, 644, 760, 1451, 1644
BstMBI GATC 6 cut(s) 530, 709, 995, 1045, 1341, 1359
BstMWI GCNNNNNNNGC 7 cut(s) 10, 127, 1484, 1585, 1594, 1607, 1643
BstNI CCWGG 1 cut(s) 118
BstNSI RCATGY 2 cut(s) 755, 1089
BstSCI CCNGG 2 cut(s) 116, 1583
BstSFI CTRYAG 1 cut(s) 1071
BstSLI GKGCMC 1 cut(s) 1585
BstV1I GCAGC 2 cut(s) 710, 1277
BstV2I GAAGAC 1 cut(s) 71
BstX2I RGATCY 2 cut(s) 709, 1341
BstYI RGATCY 2 cut(s) 709, 1341
BsuRI GGCC 1 cut(s) 1793
BtgI CCRYGG 4 cut(s) 40, 343, 465, 1318
BtsCI GGATG 5 cut(s) 264, 820, 905, 1249, 1480
BtsIMutI CAGTG 2 cut(s) 574, 1131
Cac8I GCNNGC 8 cut(s) 15, 19, 198, 1489, 1542, 1581, 1599, 1603
CaiI CAGNNNCTG 2 cut(s) 163, 1265
CciI TCATGA 1 cut(s) 1402
Cfr10I RCCGGY 1 cut(s) 1593
Cfr13I GGNCC 5 cut(s) 150, 1187, 1533, 1591, 1792
CseI GACGC 1 cut(s) 1409
Csp6I GTAC 1 cut(s) 1366
CviQI GTAC 1 cut(s) 1366
DpnI GATC 6 cut(s) 532, 711, 997, 1047, 1343, 1361
DpnII GATC 6 cut(s) 530, 709, 995, 1045, 1341, 1359
Eam1104I CTCTTC 1 cut(s) 323
EarI CTCTTC 1 cut(s) 323
Ecl136II GAGCTC 2 cut(s) 495, 1375
Eco130I CCWWGG 6 cut(s) 343, 465, 498, 782, 1677, 1796
Eco24I GRGCYC 2 cut(s) 497, 1377
Eco31I GGTCTC 1 cut(s) 1644
Eco47I GGWCC 4 cut(s) 150, 1187, 1533, 1591
Eco53kI GAGCTC 2 cut(s) 495, 1375
Eco57I CTGAAG 5 cut(s) 143, 307, 678, 1254, 1488
EcoICRI GAGCTC 2 cut(s) 495, 1375
EcoO109I RGGNCCY 2 cut(s) 1533, 1792
EcoRI GAATTC 1 cut(s) 358
EcoRII CCWGG 1 cut(s) 116
EcoT14I CCWWGG 6 cut(s) 343, 465, 498, 782, 1677, 1796
EcoT38I GRGCYC 2 cut(s) 497, 1377
ErhI CCWWGG 6 cut(s) 343, 465, 498, 782, 1677, 1796
FalI AAGNNNNNCTT 4 cut(s) 1594, 1626, 1674, 1706
FaqI GGGAC 2 cut(s) 362, 375
FauNDI CATATG 1 cut(s) 804
FbaI TGATCA 2 cut(s) 1045, 1359
Fnu4HI GCNGC 4 cut(s) 128, 699, 1291, 1381
FokI GGATG 5 cut(s) 271, 827, 912, 1256, 1487
FriOI GRGCYC 2 cut(s) 497, 1377
Fsp4HI GCNGC 4 cut(s) 128, 699, 1291, 1381
FspBI CTAG 6 cut(s) 387, 707, 723, 783, 947, 1638
GluI GCNGC 4 cut(s) 128, 699, 1291, 1381
GsuI CTGGAG 2 cut(s) 953, 1414
HaeIII GGCC 1 cut(s) 1793
HapII CCGG 3 cut(s) 363, 1585, 1594
HgaI GACGC 1 cut(s) 1409
HindIII AAGCTT 3 cut(s) 1542, 1688, 1760
HinfI GANTC 7 cut(s) 105, 230, 450, 765, 1062, 1262, 1738
HpaII CCGG 3 cut(s) 363, 1585, 1594
HphI GGTGA 1 cut(s) 229
Hpy166II GTNNAC 1 cut(s) 314
Hpy188III TCNNGA 8 cut(s) 148, 227, 325, 677, 759, 1241, 1403, 1415
Hpy8I GTNNAC 1 cut(s) 314
HpyAV CCTTC 4 cut(s) 874, 878, 973, 1147
HpyCH4III ACNGT 4 cut(s) 44, 1315, 1462, 1626
HpyCH4IV ACGT 1 cut(s) 112
HpyF10VI GCNNNNNNNGC 7 cut(s) 10, 127, 1484, 1585, 1594, 1607, 1643
HpySE526I ACGT 1 cut(s) 112
Ksp22I TGATCA 2 cut(s) 1045, 1359
Kzo9I GATC 6 cut(s) 530, 709, 995, 1045, 1341, 1359
LmnI GCTCC 3 cut(s) 345, 371, 500
Lsp1109I GCAGC 2 cut(s) 710, 1277
LweI GCATC 3 cut(s) 64, 262, 1465
MaeI CTAG 6 cut(s) 387, 707, 723, 783, 947, 1638
MaeII ACGT 1 cut(s) 112
MaeIII GTNAC 2 cut(s) 927, 1309
MalI GATC 6 cut(s) 532, 711, 997, 1047, 1343, 1361
MbiI CCGCTC 1 cut(s) 894
MboI GATC 6 cut(s) 530, 709, 995, 1045, 1341, 1359
MboII GAAGA 9 cut(s) 76, 313, 340, 367, 557, 647, 848, 863, 1391
MflI RGATCY 2 cut(s) 709, 1341
MhlI GDGCHC 4 cut(s) 497, 555, 1377, 1585
MluCI AATT 6 cut(s) 358, 578, 617, 974, 1040, 1449
MlyI GAGTC 3 cut(s) 459, 1271, 1747
MmeI TCCRAC 1 cut(s) 82
MroXI GAANNNNTTC 2 cut(s) 1146, 1450
MseI TTAA 4 cut(s) 621, 1056, 1305, 1425
MslI CAYNNNNRTG 1 cut(s) 1143
MspI CCGG 3 cut(s) 363, 1585, 1594
MspR9I CCNGG 2 cut(s) 118, 1585
Mva1269I GAATGC 1 cut(s) 908
MvaI CCWGG 1 cut(s) 118
MwoI GCNNNNNNNGC 7 cut(s) 10, 127, 1484, 1585, 1594, 1607, 1643
NciI CCSGG 1 cut(s) 1585
NcoI CCATGG 2 cut(s) 343, 465
NdeI CATATG 1 cut(s) 804
NdeII GATC 6 cut(s) 530, 709, 995, 1045, 1341, 1359
NlaIV GGNNCC 5 cut(s) 367, 565, 1116, 1535, 1794
NmuCI GTSAC 1 cut(s) 1309
NspI RCATGY 2 cut(s) 755, 1089
PagI TCATGA 1 cut(s) 1402
PciI ACATGT 1 cut(s) 751
PctI GAATGC 1 cut(s) 908
PdmI GAANNNNTTC 2 cut(s) 1146, 1450
PfeI GAWTC 4 cut(s) 105, 230, 765, 1062
PflMI CCANNNNNTGG 1 cut(s) 240
PkrI GCNGC 4 cut(s) 129, 700, 1292, 1382
PleI GAGTC 3 cut(s) 458, 1270, 1746
PpsI GAGTC 3 cut(s) 458, 1270, 1746
Ppu21I YACGTR 1 cut(s) 113
PpuMI RGGWCCY 1 cut(s) 1533
PscI ACATGT 1 cut(s) 751
Psp124BI GAGCTC 2 cut(s) 497, 1377
Psp5II RGGWCCY 1 cut(s) 1533
Psp6I CCWGG 1 cut(s) 116
PspGI CCWGG 1 cut(s) 116
PspN4I GGNNCC 5 cut(s) 367, 565, 1116, 1535, 1794
PspPI GGNCC 5 cut(s) 150, 1187, 1533, 1591, 1792
PspPPI RGGWCCY 1 cut(s) 1533
PstNI CAGNNNCTG 2 cut(s) 163, 1265
PsuI RGATCY 2 cut(s) 709, 1341
RsaI GTAC 1 cut(s) 1367
RsaNI GTAC 1 cut(s) 1366
RseI CAYNNNNRTG 1 cut(s) 1143
SacI GAGCTC 2 cut(s) 497, 1377
SaqAI TTAA 4 cut(s) 621, 1056, 1305, 1425
SatI GCNGC 4 cut(s) 128, 699, 1291, 1381
Sau3AI GATC 6 cut(s) 530, 709, 995, 1045, 1341, 1359
Sau96I GGNCC 5 cut(s) 150, 1187, 1533, 1591, 1792
ScaI AGTACT 1 cut(s) 1367
SchI GAGTC 3 cut(s) 459, 1271, 1747
ScrFI CCNGG 2 cut(s) 118, 1585
SduI GDGCHC 4 cut(s) 497, 555, 1377, 1585
SfaNI GCATC 3 cut(s) 64, 262, 1465
SfcI CTRYAG 1 cut(s) 1071
SinI GGWCC 4 cut(s) 150, 1187, 1533, 1591
SmiMI CAYNNNNRTG 1 cut(s) 1143
SmlI CTYRAG 3 cut(s) 191, 1415, 1763
SmoI CTYRAG 3 cut(s) 191, 1415, 1763
Sse9I AATT 6 cut(s) 358, 578, 617, 974, 1040, 1449
SsiI CCGC 3 cut(s) 127, 894, 1381
SspMI CTAG 6 cut(s) 387, 707, 723, 783, 947, 1638
SstI GAGCTC 2 cut(s) 497, 1377
StyD4I CCNGG 2 cut(s) 116, 1583
StyI CCWWGG 6 cut(s) 343, 465, 498, 782, 1677, 1796
TaaI ACNGT 4 cut(s) 44, 1315, 1462, 1626
TaiI ACGT 1 cut(s) 115
TaqI TCGA 2 cut(s) 678, 1520
TasI AATT 6 cut(s) 358, 578, 617, 974, 1040, 1449
TatI WGTACW 1 cut(s) 1365
TauI GCSGC 2 cut(s) 130, 1383
TfiI GAWTC 4 cut(s) 105, 230, 765, 1062
Tru1I TTAA 4 cut(s) 621, 1056, 1305, 1425
Tru9I TTAA 4 cut(s) 621, 1056, 1305, 1425
TscAI CASTG 2 cut(s) 574, 1131
TseFI GTSAC 1 cut(s) 1309
TseI GCWGC 2 cut(s) 698, 1290
Tsp45I GTSAC 1 cut(s) 1309
TspDTI ATGAA 7 cut(s) 110, 558, 855, 1026, 1155, 1391, 1577
TspGWI ACGGA 1 cut(s) 1307
TspRI CASTG 2 cut(s) 574, 1131
Van91I CCANNNNNTGG 1 cut(s) 240
VpaK11BI GGWCC 4 cut(s) 150, 1187, 1533, 1591
XapI RAATTY 3 cut(s) 358, 617, 1040
XceI RCATGY 2 cut(s) 755, 1089
XmaJI CCTAGG 1 cut(s) 782
XmnI GAANNNNTTC 2 cut(s) 1146, 1450
XspI CTAG 6 cut(s) 387, 707, 723, 783, 947, 1638
ZrmI AGTACT 1 cut(s) 1367
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.