Rroxscaffold_7G00208220

B3 domain-containing protein Os01g0234100-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0001540
Physical Location & Seq
Forward (+)
58205890 .. 58209485
3596 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00208220.1

Sequence Viewer

Length: 1359 bp
ATGGCGATTGTTCAAGAACAGAAACAGAGCTCCAAACAGGTGTTGCAGATTAAGAGGGGAAGATCACCAAACAAGGAAGAAAAGCTGTTGAAGAGAAAATCTTCATGTCTCAAGCAGACAACCGAGAACAATATGGTCAATGCTAAATCCATCAAGTCTGAAAGTAGTCTTAAACCAAAGAGATTAAAGAGAGCGACAGAAGAAAGTGTATATGACACTTCCCAAGCTCAGCTCTCTGTTCTGGAAAGAGCAAAAGAGATTGAAGCAAGTTTAGCACCAGAGTTTCCTACACTGATCAAAGTTATGCTACCGTCACATGTTACGGGAGGATTCTGGCTTGGTCTTCCTAAGAAATTCTGTTGTGTGCATTTGCCAAAACATGACACGATGATTGCTTTGGAAGATGAAAGTGGGGAAGAATACCAAACCAAATATCTTGCGGACAAGGTTGGACTAAGTGGTGGATGGAGAGGCTTTTCGATTGCTCACAAGTTAGTGGAGGGAGATGTGGTAGTTTTCCATCGTGTCACTCCTTCAAAGTTCAAGGTGTATATTGTTCGATCAAAGGGTTCAGATGAATCGGATTGCTCTCTTGGCCTTATGAACCTAGGTGTTTGCATTAAAGAAATGGATACTGGGGACATTACTACTTGTGAAGGGGCAGAATATGAGGATATTAGACCTATTTCGGTGGAAAATCCTCAAAACTATAACACCATAGCCTGCAATACTAAATCCAGTCCGATTGCTGATCTGTCTGAAAATGATACTGGAAGTCTTGGTTCTGAAGTGTTTGATGGAATTAGACTCGCAGAGTCTGTGATTCCTTTCAAAGAAGTGAGCTCCTTGGAGAACTTCAACATCATTGTTGATGGTTTCATCATAAATTCCGAGTTTCCCAAACCAGTTTTGGCGAAGTACTATGACCTTTGCTGCAGTCAAAACGCATACCTTCACGAACATCTTCTGGATGGTCTTAATTGGCAACTCATAACCGGGGCAATTTCTGAAATAACCACCATCGCAGAAGGCATAAGAACCTGCAAGGCTTCCACATTAGAATGTAATTTTCCAACTTGGAATAAGACTCTACAGGCCTTTGAGGTGTTTGGCATGAATGTTGGTTTCCTGCGTACCCGGCTAGACAAGCTTGCAAGTCTCGCTTCAGAGTCAAAAAGAGGTAAAGAGGCAAGACATGAAAGGGAACAAGCAGAAGTAGAGATGAGAACCTTAGAGGCAAAGCTGTTGGAAGTGAAAAGGACCATAAGCAGGCTGTTGGAAGTAGAGACTGAGACTCAAAATGTGAGTACAGAAAAGCTTGAGGATATGCTCCAACAAGTGGCTAAGGCCCCTTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

452

Amino Acids

50.61

Weight (kDa)

5.91

Isoelectric Point (pI)

42.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 99 - 187 7.9e-16 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1049
AccB7I CCANNNNNTGG 1 cut(s) 1339
AciI CCGC 1 cut(s) 440
AcsI RAATTY 2 cut(s) 353, 886
AcuI CTGAAG 2 cut(s) 807, 1149
AfaI GTAC 3 cut(s) 920, 1135, 1309
AfiI CCNNNNNNNGG 2 cut(s) 1269, 1339
AflIII ACRYGT 1 cut(s) 316
AgsI TTSAA 7 cut(s) 14, 91, 263, 537, 544, 832, 859
AluBI AGCT 8 cut(s) 30, 85, 227, 232, 843, 1150, 1243, 1318
AluI AGCT 8 cut(s) 30, 85, 227, 232, 843, 1150, 1243, 1318
Alw21I GWGCWC 2 cut(s) 32, 845
Alw26I GTCTC 4 cut(s) 113, 1163, 1280, 1286
AlwNI CAGNNNCTG 1 cut(s) 818
AoxI GGCC 3 cut(s) 595, 1095, 1347
ApeKI GCWGC 1 cut(s) 933
ApoI RAATTY 2 cut(s) 353, 886
Asp700I GAANNNNTTC 2 cut(s) 100, 963
AspA2I CCTAGG 1 cut(s) 607
AspS9I GGNCC 2 cut(s) 1260, 1348
AsuC2I CCSGG 2 cut(s) 997, 1138
AsuHPI GGTGA 1 cut(s) 57
AvaII GGWCC 1 cut(s) 1260
AvrII CCTAGG 1 cut(s) 607
BanII GRGCYC 2 cut(s) 32, 845
BbsI GAAGAC 1 cut(s) 335
Bbv12I GWGCWC 2 cut(s) 32, 845
BbvI GCAGC 1 cut(s) 920
BccI CCATC 7 cut(s) 158, 459, 528, 791, 866, 965, 1028
BciVI GTATCC 1 cut(s) 625
BclI TGATCA 1 cut(s) 294
BcnI CCSGG 2 cut(s) 997, 1138
BcoDI GTCTC 4 cut(s) 113, 1163, 1280, 1286
BfaI CTAG 2 cut(s) 608, 1142
BfmI CTRYAG 2 cut(s) 934, 1091
BfuAI ACCTGC 1 cut(s) 1049
BfuI GTATCC 1 cut(s) 625
BisI GCNGC 1 cut(s) 934
BlnI CCTAGG 1 cut(s) 607
BlpI GCTNAGC 1 cut(s) 228
BlsI GCNGC 1 cut(s) 935
BmcAI AGTACT 1 cut(s) 920
Bme1390I CCNGG 2 cut(s) 997, 1138
Bme18I GGWCC 1 cut(s) 1260
BmgT120I GGNCC 2 cut(s) 1260, 1348
BmiI GGNNCC 1 cut(s) 1350
BmrFI CCNGG 2 cut(s) 997, 1138
BmrI ACTGGG 1 cut(s) 645
BmuI ACTGGG 1 cut(s) 645
BpiI GAAGAC 1 cut(s) 335
Bpu10I CCTNAGC 1 cut(s) 1344
Bpu1102I GCTNAGC 1 cut(s) 228
BpuEI CTTGAG 2 cut(s) 95, 1340
BpuMI CCSGG 2 cut(s) 997, 1138
BsaJI CCNNGG 4 cut(s) 607, 846, 996, 1352
BsaXI ACNNNNNCTCC 2 cut(s) 495, 525
Bsc4I CCNNNNNNNGG 2 cut(s) 1269, 1339
Bse1I ACTGG 4 cut(s) 640, 738, 775, 905
BseDI CCNNGG 4 cut(s) 607, 846, 996, 1352
BseGI GGATG 2 cut(s) 470, 976
BseLI CCNNNNNNNGG 2 cut(s) 1269, 1339
BseMII CTCAG 2 cut(s) 242, 1281
BseNI ACTGG 4 cut(s) 640, 738, 775, 905
BseXI GCAGC 1 cut(s) 920
BshFI GGCC 3 cut(s) 597, 1097, 1349
BsiHKAI GWGCWC 2 cut(s) 32, 845
BsiSI CCGG 2 cut(s) 996, 1138
BslFI GGGAC 1 cut(s) 653
BslI CCNNNNNNNGG 2 cut(s) 1269, 1339
BsmAI GTCTC 4 cut(s) 113, 1163, 1280, 1286
BsmFI GGGAC 1 cut(s) 653
BsnI GGCC 3 cut(s) 597, 1097, 1349
Bsp1286I GDGCHC 2 cut(s) 32, 845
Bsp143I GATC 4 cut(s) 62, 294, 560, 751
Bsp1720I GCTNAGC 1 cut(s) 228
BspACI CCGC 1 cut(s) 440
BspANI GGCC 3 cut(s) 597, 1097, 1349
BspCNI CTCAG 2 cut(s) 241, 1282
BspLI GGNNCC 1 cut(s) 1350
BspMAI CTGCAG 1 cut(s) 938
BspMI ACCTGC 1 cut(s) 1049
BsrI ACTGG 4 cut(s) 640, 738, 775, 905
BssECI CCNNGG 4 cut(s) 607, 846, 996, 1352
BssMI GATC 4 cut(s) 62, 294, 560, 751
BssT1I CCWWGG 3 cut(s) 607, 846, 1352
Bst4CI ACNGT 1 cut(s) 312
Bst6I CTCTTC 1 cut(s) 86
BstC8I GCNNGC 3 cut(s) 724, 1152, 1271
BstDEI CTNAG 6 cut(s) 228, 348, 455, 1231, 1290, 1344
BstF5I GGATG 2 cut(s) 470, 976
BstKTI GATC 4 cut(s) 65, 297, 563, 754
BstMAI GTCTC 4 cut(s) 113, 1163, 1280, 1286
BstMBI GATC 4 cut(s) 62, 294, 560, 751
BstMWI GCNNNNNNNGC 5 cut(s) 272, 594, 1138, 1147, 1160
BstNSI RCATGY 1 cut(s) 320
BstSCI CCNGG 2 cut(s) 995, 1136
BstSFI CTRYAG 2 cut(s) 934, 1091
BstV1I GCAGC 1 cut(s) 920
BstV2I GAAGAC 1 cut(s) 335
BsuI GTATCC 1 cut(s) 625
BsuRI GGCC 3 cut(s) 597, 1097, 1349
BtgZI GCGATG 1 cut(s) 1006
BtsCI GGATG 2 cut(s) 470, 976
BtsIMutI CAGTG 1 cut(s) 290
BveI ACCTGC 1 cut(s) 1049
Cac8I GCNNGC 3 cut(s) 724, 1152, 1271
CaiI CAGNNNCTG 1 cut(s) 818
Cfr13I GGNCC 2 cut(s) 1260, 1348
Csp6I GTAC 3 cut(s) 919, 1134, 1308
CviAII CATG 5 cut(s) 105, 317, 380, 1114, 1196
CviQI GTAC 3 cut(s) 919, 1134, 1308
DdeI CTNAG 6 cut(s) 228, 348, 455, 1231, 1290, 1344
DpnI GATC 4 cut(s) 64, 296, 562, 753
DpnII GATC 4 cut(s) 62, 294, 560, 751
Eam1104I CTCTTC 1 cut(s) 86
EarI CTCTTC 1 cut(s) 86
Ecl136II GAGCTC 2 cut(s) 30, 843
Eco130I CCWWGG 3 cut(s) 607, 846, 1352
Eco147I AGGCCT 1 cut(s) 1097
Eco24I GRGCYC 2 cut(s) 32, 845
Eco47I GGWCC 1 cut(s) 1260
Eco53kI GAGCTC 2 cut(s) 30, 843
Eco57I CTGAAG 2 cut(s) 807, 1149
EcoICRI GAGCTC 2 cut(s) 30, 843
EcoO109I RGGNCCY 1 cut(s) 1348
EcoT14I CCWWGG 3 cut(s) 607, 846, 1352
EcoT38I GRGCYC 2 cut(s) 32, 845
ErhI CCWWGG 3 cut(s) 607, 846, 1352
FaeI CATG 5 cut(s) 108, 320, 383, 1117, 1199
FalI AAGNNNNNCTT 2 cut(s) 1147, 1179
FaqI GGGAC 1 cut(s) 653
FatI CATG 5 cut(s) 104, 316, 379, 1113, 1195
FbaI TGATCA 1 cut(s) 294
Fnu4HI GCNGC 1 cut(s) 934
FokI GGATG 2 cut(s) 477, 983
FriOI GRGCYC 2 cut(s) 32, 845
Fsp4HI GCNGC 1 cut(s) 934
FspBI CTAG 2 cut(s) 608, 1142
GluI GCNGC 1 cut(s) 934
HaeIII GGCC 3 cut(s) 597, 1097, 1349
HapII CCGG 2 cut(s) 996, 1138
Hin1II CATG 5 cut(s) 108, 320, 383, 1117, 1199
HindIII AAGCTT 2 cut(s) 1148, 1316
HinfI GANTC 8 cut(s) 330, 578, 807, 815, 823, 1087, 1169, 1294
HpaII CCGG 2 cut(s) 996, 1138
HphI GGTGA 1 cut(s) 57
Hpy188I TCNGA 9 cut(s) 160, 574, 583, 744, 760, 787, 892, 1009, 1168
Hpy188III TCNNGA 4 cut(s) 14, 242, 956, 968
HpyAV CCTTC 4 cut(s) 543, 650, 962, 1022
HpyCH4III ACNGT 1 cut(s) 312
HpyCH4V TGCA 7 cut(s) 46, 367, 618, 726, 936, 1044, 1154
HpyF10VI GCNNNNNNNGC 5 cut(s) 272, 594, 1138, 1147, 1160
HpyF3I CTNAG 6 cut(s) 228, 348, 455, 1231, 1290, 1344
Hsp92II CATG 5 cut(s) 108, 320, 383, 1117, 1199
Ksp22I TGATCA 1 cut(s) 294
Kzo9I GATC 4 cut(s) 62, 294, 560, 751
LmnI GCTCC 3 cut(s) 35, 848, 1335
Lsp1109I GCAGC 1 cut(s) 920
MaeI CTAG 2 cut(s) 608, 1142
MaeIII GTNAC 3 cut(s) 312, 319, 526
MalI GATC 4 cut(s) 64, 296, 562, 753
MboI GATC 4 cut(s) 62, 294, 560, 751
MboII GAAGA 9 cut(s) 72, 89, 93, 103, 212, 335, 413, 428, 956
MhlI GDGCHC 2 cut(s) 32, 845
MluCI AATT 6 cut(s) 353, 801, 886, 979, 1002, 1066
MlyI GAGTC 5 cut(s) 801, 824, 1081, 1178, 1288
MmeI TCCRAC 5 cut(s) 430, 1097, 1227, 1257, 1357
MroXI GAANNNNTTC 2 cut(s) 100, 963
MseI TTAA 5 cut(s) 51, 171, 185, 621, 978
MslI CAYNNNNRTG 1 cut(s) 1060
MspI CCGG 2 cut(s) 996, 1138
MspR9I CCNGG 2 cut(s) 997, 1138
MwoI GCNNNNNNNGC 5 cut(s) 272, 594, 1138, 1147, 1160
NciI CCSGG 2 cut(s) 997, 1138
NdeII GATC 4 cut(s) 62, 294, 560, 751
NlaIII CATG 5 cut(s) 108, 320, 383, 1117, 1199
NlaIV GGNNCC 1 cut(s) 1350
NmuCI GTSAC 2 cut(s) 312, 526
NspI RCATGY 1 cut(s) 320
PceI AGGCCT 1 cut(s) 1097
PciI ACATGT 1 cut(s) 316
PdmI GAANNNNTTC 2 cut(s) 100, 963
PfeI GAWTC 3 cut(s) 330, 578, 823
PflMI CCANNNNNTGG 1 cut(s) 1339
PkrI GCNGC 1 cut(s) 935
PleI GAGTC 5 cut(s) 801, 823, 1081, 1177, 1288
PpsI GAGTC 5 cut(s) 801, 823, 1081, 1177, 1288
PscI ACATGT 1 cut(s) 316
Psp124BI GAGCTC 2 cut(s) 32, 845
PspN4I GGNNCC 1 cut(s) 1350
PspPI GGNCC 2 cut(s) 1260, 1348
PstI CTGCAG 1 cut(s) 938
PstNI CAGNNNCTG 1 cut(s) 818
RsaI GTAC 3 cut(s) 920, 1135, 1309
RsaNI GTAC 3 cut(s) 919, 1134, 1308
RseI CAYNNNNRTG 1 cut(s) 1060
SacI GAGCTC 2 cut(s) 32, 845
SaqAI TTAA 5 cut(s) 51, 171, 185, 621, 978
SatI GCNGC 1 cut(s) 934
Sau3AI GATC 4 cut(s) 62, 294, 560, 751
Sau96I GGNCC 2 cut(s) 1260, 1348
ScaI AGTACT 1 cut(s) 920
SchI GAGTC 5 cut(s) 801, 824, 1081, 1178, 1288
ScrFI CCNGG 2 cut(s) 997, 1138
SduI GDGCHC 2 cut(s) 32, 845
SfcI CTRYAG 2 cut(s) 934, 1091
SinI GGWCC 1 cut(s) 1260
SmiMI CAYNNNNRTG 1 cut(s) 1060
SmlI CTYRAG 2 cut(s) 110, 1319
SmoI CTYRAG 2 cut(s) 110, 1319
Sse9I AATT 6 cut(s) 353, 801, 886, 979, 1002, 1066
SseBI AGGCCT 1 cut(s) 1097
SsiI CCGC 1 cut(s) 440
SspMI CTAG 2 cut(s) 608, 1142
SstI GAGCTC 2 cut(s) 32, 845
StuI AGGCCT 1 cut(s) 1097
StyD4I CCNGG 2 cut(s) 995, 1136
StyI CCWWGG 3 cut(s) 607, 846, 1352
TaaI ACNGT 1 cut(s) 312
TaqI TCGA 2 cut(s) 479, 559
TasI AATT 6 cut(s) 353, 801, 886, 979, 1002, 1066
TatI WGTACW 2 cut(s) 918, 1307
TfiI GAWTC 3 cut(s) 330, 578, 823
Tru1I TTAA 5 cut(s) 51, 171, 185, 621, 978
Tru9I TTAA 5 cut(s) 51, 171, 185, 621, 978
TscAI CASTG 1 cut(s) 297
TseFI GTSAC 2 cut(s) 312, 526
TseI GCWGC 1 cut(s) 933
Tsp45I GTSAC 2 cut(s) 312, 526
TspDTI ATGAA 7 cut(s) 93, 420, 591, 617, 868, 1130, 1212
TspRI CASTG 1 cut(s) 297
Van91I CCANNNNNTGG 1 cut(s) 1339
VpaK11BI GGWCC 1 cut(s) 1260
XapI RAATTY 2 cut(s) 353, 886
XceI RCATGY 1 cut(s) 320
XcmI CCANNNNNNNNNTGG 1 cut(s) 907
XmaJI CCTAGG 1 cut(s) 607
XmnI GAANNNNTTC 2 cut(s) 100, 963
XspI CTAG 2 cut(s) 608, 1142
ZrmI AGTACT 1 cut(s) 920
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.