Rorug05G0577800

B3 domain-containing protein Os01g0234100-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0001540
Physical Location & Seq
Forward (+)
77379890 .. 77380737
848 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0577800.1

Sequence Viewer

Length: 753 bp
ATGGGAAGGAGCAATTCCTTACAGATTCATCTCCACGAATCCGAAGTCACCACTCTTCCTTCTCCCCCACCCGCCCTGATCTTAGCTCCTCGGCTAGGATACCTACCATTGTTAGCCTCGCATTTAAAGCCTTACTTCAGCAGTGCACTTCCTCCTGGTGTTGACACTATTTGGTTCGAGTACAGAGGCTTGCCCTTGAAATGGTATATACCAACAGGAGTTCTTTTTGATCTTCTATGTGCAGAACCGGAGAGACCTTGGAATTTAACGGTGCATTTTAGAGGATATCCAGGAAACATATTGATCCGTTGTGAAGATGAAGATAGCATAAAGTGGAGCTTTATCAACTCATTAAAAGAGGCAGCATATATAATCAATGGAAACTGCAAGAATGTGATGAACATGTGTCAACCTGATCAGATGGAGCTCTGGCGCTCTGTCTTAAATGGTAATTTGGAAGCCTACCTACGGGTGAAGCAAGGCCGAGTTCCTGTTCTGTTATATGTTTGGTATGTCATTGAGGACTTTGATGACTTGGAAGATGCACCACCGATTGATAGTTGGGACAAAGTTTTTTACATAAACCAGCCTGTCGAGATACAAGGAGAAGGTAGATGCTTCACTTTACATGATGCAGTGAAAAGCCTTCTGCCAGACTATTTTGTGGAGAAATCGTTGGGCACTGCTGAATCATTGACAGTAGGTGGTGATGACAAGCAAAGAGTTTCATCCGAAGATGCAAGCGATCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

250

Amino Acids

28.43

Weight (kDa)

4.84

Isoelectric Point (pI)

43.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATG5_UblA PF20638 7 - 93 3.6e-25 Autophagy protein ATG5, UblA domain
ATG5_HBR PF20637 107 - 159 2.1e-20 Autophagy protein ATG5, alpha-helical bundle region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 72
AclWI GGATC 1 cut(s) 298
AcsI RAATTY 1 cut(s) 262
AcuI CTGAAG 1 cut(s) 121
AfaI GTAC 1 cut(s) 182
AfiI CCNNNNNNNGG 3 cut(s) 95, 201, 468
AflIII ACRYGT 1 cut(s) 402
AgsI TTSAA 1 cut(s) 199
AjnI CCWGG 2 cut(s) 154, 289
AluBI AGCT 3 cut(s) 86, 339, 427
AluI AGCT 3 cut(s) 86, 339, 427
Alw21I GWGCWC 2 cut(s) 148, 429
Alw26I GTCTC 1 cut(s) 247
Alw44I GTGCAC 1 cut(s) 144
AlwI GGATC 1 cut(s) 298
AoxI GGCC 1 cut(s) 481
ApaLI GTGCAC 1 cut(s) 144
ApeKI GCWGC 1 cut(s) 362
ApoI RAATTY 1 cut(s) 262
ArsI GACNNNNNNTTYG 2 cut(s) 30, 62
AspLEI GCGC 1 cut(s) 435
AsuHPI GGTGA 3 cut(s) 40, 484, 719
BaeGI GKGCMC 2 cut(s) 148, 683
BanII GRGCYC 1 cut(s) 429
BarI GAAGNNNNNNTAC 2 cut(s) 450, 482
Bbv12I GWGCWC 2 cut(s) 148, 429
BbvI GCAGC 1 cut(s) 374
BccI CCATC 1 cut(s) 415
BciT130I CCWGG 2 cut(s) 156, 291
BciVI GTATCC 1 cut(s) 92
BclI TGATCA 1 cut(s) 415
BcoDI GTCTC 1 cut(s) 247
BfaI CTAG 1 cut(s) 95
BfoI RGCGCY 1 cut(s) 436
BfuI GTATCC 1 cut(s) 92
BisI GCNGC 1 cut(s) 363
BlsI GCNGC 1 cut(s) 364
Bme1390I CCNGG 2 cut(s) 156, 291
BmrFI CCNGG 2 cut(s) 156, 291
BmsI GCATC 4 cut(s) 532, 605, 622, 727
Bsa29I ATCGAT 1 cut(s) 748
BsaI GGTCTC 1 cut(s) 247
BsaJI CCNNGG 2 cut(s) 89, 257
BsaWI WCCGGW 1 cut(s) 247
BsaXI ACNNNNNCTCC 2 cut(s) 659, 689
Bsc4I CCNNNNNNNGG 3 cut(s) 95, 201, 468
BseBI CCWGG 2 cut(s) 156, 291
BseCI ATCGAT 1 cut(s) 748
BseDI CCNNGG 2 cut(s) 89, 257
BseGI GGATG 1 cut(s) 728
BseLI CCNNNNNNNGG 3 cut(s) 95, 201, 468
BseRI GAGGAG 1 cut(s) 78
BseSI GKGCMC 2 cut(s) 148, 683
BseXI GCAGC 1 cut(s) 374
BsgI GTGCAG 1 cut(s) 261
Bsh1285I CGRYCG 1 cut(s) 748
BshFI GGCC 1 cut(s) 483
BshVI ATCGAT 1 cut(s) 748
BsiEI CGRYCG 1 cut(s) 748
BsiHKAI GWGCWC 2 cut(s) 148, 429
BsiSI CCGG 1 cut(s) 248
BslFI GGGAC 1 cut(s) 578
BslI CCNNNNNNNGG 3 cut(s) 95, 201, 468
BsmAI GTCTC 1 cut(s) 247
BsmFI GGGAC 1 cut(s) 578
BsnI GGCC 1 cut(s) 483
Bso31I GGTCTC 1 cut(s) 247
Bsp1286I GDGCHC 3 cut(s) 148, 429, 683
Bsp143I GATC 5 cut(s) 78, 229, 303, 415, 745
BspACI CCGC 1 cut(s) 72
BspANI GGCC 1 cut(s) 483
BspDI ATCGAT 1 cut(s) 748
BspPI GGATC 1 cut(s) 298
BspTNI GGTCTC 1 cut(s) 247
BssECI CCNNGG 2 cut(s) 89, 257
BssMI GATC 5 cut(s) 78, 229, 303, 415, 745
BssT1I CCWWGG 1 cut(s) 257
Bst2UI CCWGG 2 cut(s) 156, 291
Bst4CI ACNGT 2 cut(s) 271, 700
Bst6I CTCTTC 1 cut(s) 60
BstC8I GCNNGC 2 cut(s) 191, 742
BstDEI CTNAG 1 cut(s) 82
BstENI CCTNNNNNAGG 1 cut(s) 93
BstF5I GGATG 1 cut(s) 728
BstH2I RGCGCY 1 cut(s) 436
BstHHI GCGC 1 cut(s) 435
BstKTI GATC 5 cut(s) 81, 232, 306, 418, 748
BstMAI GTCTC 1 cut(s) 247
BstMBI GATC 5 cut(s) 78, 229, 303, 415, 745
BstMCI CGRYCG 1 cut(s) 748
BstMWI GCNNNNNNNGC 1 cut(s) 127
BstNI CCWGG 2 cut(s) 156, 291
BstNSI RCATGY 1 cut(s) 406
BstSCI CCNGG 2 cut(s) 154, 289
BstSLI GKGCMC 2 cut(s) 148, 683
BstV1I GCAGC 1 cut(s) 374
Bsu15I ATCGAT 1 cut(s) 748
BsuI GTATCC 1 cut(s) 92
BsuRI GGCC 1 cut(s) 483
BsuTUI ATCGAT 1 cut(s) 748
BtsCI GGATG 1 cut(s) 728
BtsI GCAGTG 3 cut(s) 148, 642, 681
BtsIMutI CAGTG 3 cut(s) 148, 642, 681
Cac8I GCNNGC 2 cut(s) 191, 742
CfoI GCGC 1 cut(s) 435
ClaI ATCGAT 1 cut(s) 748
Csp6I GTAC 1 cut(s) 181
CviAII CATG 2 cut(s) 403, 629
CviQI GTAC 1 cut(s) 181
DdeI CTNAG 1 cut(s) 82
DpnI GATC 5 cut(s) 80, 231, 305, 417, 747
DpnII GATC 5 cut(s) 78, 229, 303, 415, 745
DraI TTTAAA 1 cut(s) 126
Eam1104I CTCTTC 1 cut(s) 60
EarI CTCTTC 1 cut(s) 60
Ecl136II GAGCTC 1 cut(s) 427
Eco130I CCWWGG 1 cut(s) 257
Eco24I GRGCYC 1 cut(s) 429
Eco31I GGTCTC 1 cut(s) 247
Eco32I GATATC 1 cut(s) 287
Eco53kI GAGCTC 1 cut(s) 427
Eco57I CTGAAG 1 cut(s) 121
EcoICRI GAGCTC 1 cut(s) 427
EcoNI CCTNNNNNAGG 1 cut(s) 93
EcoRII CCWGG 2 cut(s) 154, 289
EcoRV GATATC 1 cut(s) 287
EcoT14I CCWWGG 1 cut(s) 257
EcoT38I GRGCYC 1 cut(s) 429
ErhI CCWWGG 1 cut(s) 257
FaeI CATG 2 cut(s) 406, 632
FalI AAGNNNNNCTT 4 cut(s) 119, 151, 323, 355
FaqI GGGAC 1 cut(s) 578
FatI CATG 2 cut(s) 402, 628
FauI CCCGC 1 cut(s) 79
FbaI TGATCA 1 cut(s) 415
Fnu4HI GCNGC 1 cut(s) 363
FokI GGATG 1 cut(s) 715
FriOI GRGCYC 1 cut(s) 429
Fsp4HI GCNGC 1 cut(s) 363
FspBI CTAG 1 cut(s) 95
GlaI GCGC 1 cut(s) 434
GluI GCNGC 1 cut(s) 363
HaeII RGCGCY 1 cut(s) 436
HaeIII GGCC 1 cut(s) 483
HapII CCGG 1 cut(s) 248
HhaI GCGC 1 cut(s) 435
Hin1II CATG 2 cut(s) 406, 632
Hin6I GCGC 1 cut(s) 433
HinP1I GCGC 1 cut(s) 433
HincII GTYRAC 2 cut(s) 163, 410
HindII GTYRAC 2 cut(s) 163, 410
HinfI GANTC 3 cut(s) 25, 38, 689
HpaII CCGG 1 cut(s) 248
HphI GGTGA 3 cut(s) 40, 484, 719
Hpy166II GTNNAC 3 cut(s) 146, 163, 410
Hpy188I TCNGA 3 cut(s) 43, 420, 733
Hpy188III TCNNGA 1 cut(s) 595
Hpy8I GTNNAC 3 cut(s) 146, 163, 410
HpyAV CCTTC 3 cut(s) 69, 602, 656
HpyCH4III ACNGT 2 cut(s) 271, 700
HpyCH4V TGCA 7 cut(s) 146, 242, 274, 387, 545, 635, 740
HpyF10VI GCNNNNNNNGC 1 cut(s) 127
HpyF3I CTNAG 1 cut(s) 82
Hsp92II CATG 2 cut(s) 406, 632
HspAI GCGC 1 cut(s) 433
Ksp22I TGATCA 1 cut(s) 415
Kzo9I GATC 5 cut(s) 78, 229, 303, 415, 745
LmnI GCTCC 4 cut(s) 9, 91, 336, 424
Lsp1109I GCAGC 1 cut(s) 374
LweI GCATC 4 cut(s) 532, 605, 622, 727
MaeI CTAG 1 cut(s) 95
MaeIII GTNAC 1 cut(s) 46
MalI GATC 5 cut(s) 80, 231, 305, 417, 747
MboI GATC 5 cut(s) 78, 229, 303, 415, 745
MboII GAAGA 6 cut(s) 47, 224, 326, 332, 551, 746
MhlI GDGCHC 3 cut(s) 148, 429, 683
MluCI AATT 3 cut(s) 13, 262, 451
MnlI CCTC 7 cut(s) 99, 127, 162, 179, 275, 352, 514
MseI TTAA 4 cut(s) 125, 266, 353, 443
MspI CCGG 1 cut(s) 248
MspR9I CCNGG 2 cut(s) 156, 291
MvaI CCWGG 2 cut(s) 156, 291
MwoI GCNNNNNNNGC 1 cut(s) 127
NdeII GATC 5 cut(s) 78, 229, 303, 415, 745
NlaIII CATG 2 cut(s) 406, 632
NmeAIII GCCGAG 2 cut(s) 70, 509
NmuCI GTSAC 1 cut(s) 46
NspI RCATGY 1 cut(s) 406
PciI ACATGT 1 cut(s) 402
PfeI GAWTC 3 cut(s) 25, 38, 689
PfoI TCCNGGA 1 cut(s) 289
PkrI GCNGC 1 cut(s) 364
Ple19I CGATCG 1 cut(s) 748
PscI ACATGT 1 cut(s) 402
Psp124BI GAGCTC 1 cut(s) 429
Psp6I CCWGG 2 cut(s) 154, 289
PspGI CCWGG 2 cut(s) 154, 289
PvuI CGATCG 1 cut(s) 748
RsaI GTAC 1 cut(s) 182
RsaNI GTAC 1 cut(s) 181
SacI GAGCTC 1 cut(s) 429
SaqAI TTAA 4 cut(s) 125, 266, 353, 443
SatI GCNGC 1 cut(s) 363
Sau3AI GATC 5 cut(s) 78, 229, 303, 415, 745
ScrFI CCNGG 2 cut(s) 156, 291
SduI GDGCHC 3 cut(s) 148, 429, 683
SetI ASST 9 cut(s) 88, 105, 259, 341, 415, 429, 468, 613, 706
SfaNI GCATC 4 cut(s) 532, 605, 622, 727
Sse9I AATT 3 cut(s) 13, 262, 451
SsiI CCGC 1 cut(s) 72
SspMI CTAG 1 cut(s) 95
SstI GAGCTC 1 cut(s) 429
StyD4I CCNGG 2 cut(s) 154, 289
StyI CCWWGG 1 cut(s) 257
TaaI ACNGT 2 cut(s) 271, 700
TaqI TCGA 3 cut(s) 177, 594, 748
TasI AATT 3 cut(s) 13, 262, 451
TatI WGTACW 1 cut(s) 180
TfiI GAWTC 3 cut(s) 25, 38, 689
Tru1I TTAA 4 cut(s) 125, 266, 353, 443
Tru9I TTAA 4 cut(s) 125, 266, 353, 443
TscAI CASTG 3 cut(s) 148, 642, 688
TseFI GTSAC 1 cut(s) 46
TseI GCWGC 1 cut(s) 362
Tsp45I GTSAC 1 cut(s) 46
TspDTI ATGAA 4 cut(s) 17, 333, 413, 717
TspGWI ACGGA 1 cut(s) 296
TspRI CASTG 3 cut(s) 148, 642, 688
VneI GTGCAC 1 cut(s) 144
XagI CCTNNNNNAGG 1 cut(s) 93
XapI RAATTY 1 cut(s) 262
XceI RCATGY 1 cut(s) 406
XspI CTAG 1 cut(s) 95
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.