RchiOBHm_Chr6g0256811

B3 domain-containing protein Os01g0234100-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0001540
Physical Location & Seq
Forward (+)
12042744 .. 12046119
3376 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ23039

Sequence Viewer

Length: 1374 bp
ATGGCAGTTGTTGAAGAACACAAAGAGAGCTCATCTTGCCCACAGCCCAAGCTTGAAAAGGTGAGACAACCAAAGAACGAAGACAAAGACAAGCCCTTTTGTCAAAACTCTTCACGTCTCAAACAGATGACCAAGAACAATGTAGTCAGTGCTAAGCCAATGAAGTCTGACAGCCATAAACCAAAGACATGTAAGAAGGCCAAAGTCAAAAGCTTATATGATACATCCAAAGCTCAGTGTTCCGTTCTTGAAAGAGCAAATGAGCTTGCAGCAAACCTAGCACCAGAGTTTCCTAGCCTCGTCAAAGTTATGATGCCGTCACATGTTACTCGAGTCTTCTGGCTTGGTATTCCTAGGTCGTTCTGCCGTGAGCATATGCCAAAGGATGACACAATGATTGTCTTGGAAGATGAAAATGGGGAAGAATACCAAACGAAATATCTTGTGGCGAAGGAAGGATTGAGCGGTGGATGGAGAGCATTCTCAGCTGCTCACAAGTTACTGGAGGGAGATGTTCTAGTTTTCCACAAAGTCATGCCATCAAAATTGAAGGTGTATATTGTTAGATCAACTGGTTCAGATGAAGTGGATTGCTCTCTTGGTCTTATCAAATTTGATCATGGCATTAGACGAATCAAAGCTACAGAAGTTACTGCATGTGAAAGCTCAAAAAATGGGAAACTGGAACCTATTTCAGTGGAAACTCTGCATGAAAATGTTCAGAAGGAGAACACCACAGCCTGTAATACCATGTCTGGTCCAATATCAAACCAGTATCAAATAGATAGTGAAGTTCTTTATTCTGAAGTTCTGGATGGAATAAGGCTTTCAGAGTCTGTTATCCCTTTCAAAGAAGTGAGCTGCATTGAGAACTTTAAGGTCACAGTCAGTGGTTCAATCATAAATGCTGAGATCTCCGAACACCATCTGATCAAGTACTATGAGCTCTGCCGCAGTCAAAACTCATTTCTTCATGAGCATATTCTTGAGCGTCTTAACTCCAGTCTGGTTGCTGGAATAATTTCCGAGACGGTAAATCTTTCGGATGCCATTAGAGCCTGCAAGATTACTACTTCAGAGTGTGATTTTTGGACTTGGGATAGGACTCTGCAAGCTTCTGAGATGTTAGGCATGAATGTTGGGTTCTTGCGTGCCCGGCTGGACCAGCTTGCAAGCCTTGCTTCAAAATCAAAACTGTGTAAAGAGGCTAGACTTGCAAGAGACCAAGCAGTAAAGGATATGATAACCTTGGAAGCAAAGCTTGTAAGAGCCAGAGAGGTCATAAATAGGCTCAACTTTGAGATTGAGAGTCTAAATACAAGTTCAAAGAAGCTTGAGGCTATGTTCAAAGAAGTGGCTAAGGCCCCTTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

457

Amino Acids

51.45

Weight (kDa)

8.29

Isoelectric Point (pI)

36.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 101 - 190 1.4e-17 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 465
AciI CCGC 2 cut(s) 465, 952
AcsI RAATTY 1 cut(s) 611
AcuI CTGAAG 2 cut(s) 825, 1059
AfaI GTAC 1 cut(s) 938
AflIII ACRYGT 2 cut(s) 188, 322
AgsI TTSAA 9 cut(s) 14, 56, 251, 550, 850, 897, 1185, 1326, 1348
AjiI CACGTC 1 cut(s) 116
Alw21I GWGCWC 2 cut(s) 32, 948
Alw26I GTCTC 4 cut(s) 58, 122, 1022, 1215
AlwNI CAGNNNCTG 1 cut(s) 836
Ama87I CYCGRG 1 cut(s) 330
AoxI GGCC 2 cut(s) 198, 1362
ApeKI GCWGC 3 cut(s) 269, 488, 861
ApoI RAATTY 1 cut(s) 611
Asp700I GAANNNNTTC 2 cut(s) 717, 1021
AspA2I CCTAGG 1 cut(s) 353
AspS9I GGNCC 3 cut(s) 758, 1162, 1363
AsuC2I CCSGG 1 cut(s) 1156
AsuHPI GGTGA 1 cut(s) 73
AvaI CYCGRG 1 cut(s) 330
AvaII GGWCC 2 cut(s) 758, 1162
AvrII CCTAGG 1 cut(s) 353
BaeGI GKGCMC 1 cut(s) 1156
BanII GRGCYC 2 cut(s) 32, 948
BbsI GAAGAC 2 cut(s) 87, 328
Bbv12I GWGCWC 2 cut(s) 32, 948
BbvI GCAGC 3 cut(s) 281, 475, 848
BccI CCATC 4 cut(s) 465, 547, 809, 933
BceAI ACGGC 2 cut(s) 301, 351
BclI TGATCA 2 cut(s) 616, 930
BcnI CCSGG 1 cut(s) 1156
BcoDI GTCTC 4 cut(s) 58, 122, 1022, 1215
BfaI CTAG 5 cut(s) 278, 294, 354, 518, 1209
BfmI CTRYAG 1 cut(s) 642
BglII AGATCT 1 cut(s) 912
BisI GCNGC 4 cut(s) 270, 489, 862, 952
BlnI CCTAGG 1 cut(s) 353
BlpI GCTNAGC 1 cut(s) 153
BlsI GCNGC 4 cut(s) 271, 490, 863, 953
BmcAI AGTACT 1 cut(s) 938
Bme1390I CCNGG 1 cut(s) 1156
Bme18I GGWCC 2 cut(s) 758, 1162
BmeT110I CYCGRG 1 cut(s) 330
BmgBI CACGTC 1 cut(s) 116
BmgT120I GGNCC 3 cut(s) 758, 1162, 1363
BmiI GGNNCC 2 cut(s) 687, 1365
BmrFI CCNGG 1 cut(s) 1156
BmsI GCATC 2 cut(s) 303, 1036
BpiI GAAGAC 2 cut(s) 87, 328
BpmI CTGGAG 2 cut(s) 524, 985
Bpu10I CCTNAGC 1 cut(s) 1359
Bpu1102I GCTNAGC 1 cut(s) 153
BpuEI CTTGAG 2 cut(s) 1007, 1355
BpuMI CCSGG 1 cut(s) 1156
BsaI GGTCTC 1 cut(s) 1215
BsaJI CCNNGG 3 cut(s) 353, 1248, 1367
Bse1I ACTGG 5 cut(s) 507, 577, 687, 772, 1002
BseDI CCNNGG 3 cut(s) 353, 1248, 1367
BseGI GGATG 5 cut(s) 224, 391, 476, 820, 1051
BseMII CTCAG 4 cut(s) 248, 498, 900, 1110
BseNI ACTGG 5 cut(s) 507, 577, 687, 772, 1002
BseSI GKGCMC 1 cut(s) 1156
BseXI GCAGC 3 cut(s) 281, 475, 848
BshFI GGCC 2 cut(s) 200, 1364
BsiHKAI GWGCWC 2 cut(s) 32, 948
BsiHKCI CYCGRG 1 cut(s) 330
BsiSI CCGG 1 cut(s) 1156
BsmAI GTCTC 4 cut(s) 58, 122, 1022, 1215
BsmBI CGTCTC 2 cut(s) 122, 1022
BsmI GAATGC 1 cut(s) 479
BsnI GGCC 2 cut(s) 200, 1364
Bso31I GGTCTC 1 cut(s) 1215
BsoBI CYCGRG 1 cut(s) 330
Bsp1286I GDGCHC 3 cut(s) 32, 948, 1156
Bsp143I GATC 4 cut(s) 566, 616, 912, 930
Bsp1720I GCTNAGC 1 cut(s) 153
BspACI CCGC 2 cut(s) 465, 952
BspANI GGCC 2 cut(s) 200, 1364
BspCNI CTCAG 4 cut(s) 247, 497, 901, 1111
BspHI TCATGA 1 cut(s) 973
BspLI GGNNCC 2 cut(s) 687, 1365
BspTNI GGTCTC 1 cut(s) 1215
BsrBI CCGCTC 1 cut(s) 465
BsrI ACTGG 5 cut(s) 507, 577, 687, 772, 1002
BssECI CCNNGG 3 cut(s) 353, 1248, 1367
BssMI GATC 4 cut(s) 566, 616, 912, 930
BssT1I CCWWGG 3 cut(s) 353, 1248, 1367
Bst4CI ACNGT 3 cut(s) 886, 1033, 1197
Bst6I CTCTTC 1 cut(s) 115
BstAPI GCANNNNNTGC 1 cut(s) 1178
BstC8I GCNNGC 6 cut(s) 267, 1060, 1113, 1152, 1170, 1174
BstDEI CTNAG 6 cut(s) 153, 234, 484, 909, 1119, 1359
BstF5I GGATG 5 cut(s) 224, 391, 476, 820, 1051
BstKTI GATC 4 cut(s) 569, 619, 915, 933
BstMAI GTCTC 4 cut(s) 58, 122, 1022, 1215
BstMBI GATC 4 cut(s) 566, 616, 912, 930
BstMWI GCNNNNNNNGC 8 cut(s) 36, 278, 485, 1055, 1156, 1165, 1178, 1214
BstNSI RCATGY 3 cut(s) 192, 326, 660
BstSCI CCNGG 1 cut(s) 1154
BstSFI CTRYAG 1 cut(s) 642
BstSLI GKGCMC 1 cut(s) 1156
BstV1I GCAGC 3 cut(s) 281, 475, 848
BstV2I GAAGAC 2 cut(s) 87, 328
BstX2I RGATCY 1 cut(s) 912
BstYI RGATCY 1 cut(s) 912
BsuRI GGCC 2 cut(s) 200, 1364
BtrI CACGTC 1 cut(s) 116
BtsCI GGATG 5 cut(s) 224, 391, 476, 820, 1051
BtsIMutI CAGTG 4 cut(s) 154, 242, 702, 895
Cac8I GCNNGC 6 cut(s) 267, 1060, 1113, 1152, 1170, 1174
CaiI CAGNNNCTG 1 cut(s) 836
CciI TCATGA 1 cut(s) 973
Cfr13I GGNCC 3 cut(s) 758, 1162, 1363
CseI GACGC 1 cut(s) 980
Csp6I GTAC 1 cut(s) 937
CviAII CATG 9 cut(s) 189, 323, 535, 620, 657, 710, 751, 974, 1132
CviQI GTAC 1 cut(s) 937
DdeI CTNAG 6 cut(s) 153, 234, 484, 909, 1119, 1359
DpnI GATC 4 cut(s) 568, 618, 914, 932
DpnII GATC 4 cut(s) 566, 616, 912, 930
Eam1104I CTCTTC 1 cut(s) 115
EarI CTCTTC 1 cut(s) 115
Ecl136II GAGCTC 2 cut(s) 30, 946
Eco130I CCWWGG 3 cut(s) 353, 1248, 1367
Eco24I GRGCYC 2 cut(s) 32, 948
Eco31I GGTCTC 1 cut(s) 1215
Eco47I GGWCC 2 cut(s) 758, 1162
Eco53kI GAGCTC 2 cut(s) 30, 946
Eco57I CTGAAG 2 cut(s) 825, 1059
Eco88I CYCGRG 1 cut(s) 330
EcoICRI GAGCTC 2 cut(s) 30, 946
EcoO109I RGGNCCY 1 cut(s) 1363
EcoT14I CCWWGG 3 cut(s) 353, 1248, 1367
EcoT38I GRGCYC 2 cut(s) 32, 948
ErhI CCWWGG 3 cut(s) 353, 1248, 1367
Esp3I CGTCTC 2 cut(s) 122, 1022
FaeI CATG 9 cut(s) 192, 326, 538, 623, 660, 713, 754, 977, 1135
FalI AAGNNNNNCTT 4 cut(s) 1165, 1197, 1245, 1277
FatI CATG 9 cut(s) 188, 322, 534, 619, 656, 709, 750, 973, 1131
FauNDI CATATG 1 cut(s) 375
FbaI TGATCA 2 cut(s) 616, 930
Fnu4HI GCNGC 4 cut(s) 270, 489, 862, 952
FokI GGATG 5 cut(s) 211, 398, 483, 827, 1058
FriOI GRGCYC 2 cut(s) 32, 948
Fsp4HI GCNGC 4 cut(s) 270, 489, 862, 952
FspBI CTAG 5 cut(s) 278, 294, 354, 518, 1209
GluI GCNGC 4 cut(s) 270, 489, 862, 952
GsuI CTGGAG 2 cut(s) 524, 985
HaeIII GGCC 2 cut(s) 200, 1364
HapII CCGG 1 cut(s) 1156
HgaI GACGC 1 cut(s) 980
Hin1II CATG 9 cut(s) 192, 326, 538, 623, 660, 713, 754, 977, 1135
HindIII AAGCTT 5 cut(s) 50, 211, 1113, 1259, 1331
HinfI GANTC 5 cut(s) 333, 633, 833, 1105, 1309
HpaII CCGG 1 cut(s) 1156
HphI GGTGA 1 cut(s) 73
Hpy188III TCNNGA 4 cut(s) 248, 812, 974, 986
HpyAV CCTTC 5 cut(s) 190, 445, 449, 544, 718
HpyCH4III ACNGT 3 cut(s) 886, 1033, 1197
HpyCH4IV ACGT 1 cut(s) 115
HpyCH4V TGCA 8 cut(s) 269, 656, 709, 864, 1062, 1111, 1172, 1217
HpyF10VI GCNNNNNNNGC 8 cut(s) 36, 278, 485, 1055, 1156, 1165, 1178, 1214
HpyF3I CTNAG 6 cut(s) 153, 234, 484, 909, 1119, 1359
HpySE526I ACGT 1 cut(s) 115
Hsp92II CATG 9 cut(s) 192, 326, 538, 623, 660, 713, 754, 977, 1135
Ksp22I TGATCA 2 cut(s) 616, 930
Kzo9I GATC 4 cut(s) 566, 616, 912, 930
Lsp1109I GCAGC 3 cut(s) 281, 475, 848
LweI GCATC 2 cut(s) 303, 1036
MaeI CTAG 5 cut(s) 278, 294, 354, 518, 1209
MaeII ACGT 1 cut(s) 115
MaeIII GTNAC 5 cut(s) 318, 325, 498, 649, 880
MalI GATC 4 cut(s) 568, 618, 914, 932
MbiI CCGCTC 1 cut(s) 465
MboI GATC 4 cut(s) 566, 616, 912, 930
MboII GAAGA 7 cut(s) 26, 92, 102, 328, 419, 434, 962
MflI RGATCY 1 cut(s) 912
MhlI GDGCHC 3 cut(s) 32, 948, 1156
MluCI AATT 3 cut(s) 545, 611, 1020
MlyI GAGTC 4 cut(s) 342, 842, 1099, 1318
MnlI CCTC 5 cut(s) 308, 499, 1198, 1270, 1330
MroXI GAANNNNTTC 2 cut(s) 717, 1021
MseI TTAA 2 cut(s) 876, 996
MslI CAYNNNNRTG 1 cut(s) 714
MspA1I CMGCKG 1 cut(s) 488
MspI CCGG 1 cut(s) 1156
MspR9I CCNGG 1 cut(s) 1156
Mva1269I GAATGC 1 cut(s) 479
MwoI GCNNNNNNNGC 8 cut(s) 36, 278, 485, 1055, 1156, 1165, 1178, 1214
NciI CCSGG 1 cut(s) 1156
NdeI CATATG 1 cut(s) 375
NdeII GATC 4 cut(s) 566, 616, 912, 930
NlaIII CATG 9 cut(s) 192, 326, 538, 623, 660, 713, 754, 977, 1135
NlaIV GGNNCC 2 cut(s) 687, 1365
NmuCI GTSAC 2 cut(s) 318, 880
NspI RCATGY 3 cut(s) 192, 326, 660
PaeR7I CTCGAG 1 cut(s) 330
PagI TCATGA 1 cut(s) 973
PciI ACATGT 2 cut(s) 188, 322
PctI GAATGC 1 cut(s) 479
PdmI GAANNNNTTC 2 cut(s) 717, 1021
PfeI GAWTC 1 cut(s) 633
PkrI GCNGC 4 cut(s) 271, 490, 863, 953
PleI GAGTC 4 cut(s) 341, 841, 1099, 1317
PpsI GAGTC 4 cut(s) 341, 841, 1099, 1317
PscI ACATGT 2 cut(s) 188, 322
Psp124BI GAGCTC 2 cut(s) 32, 948
PspN4I GGNNCC 2 cut(s) 687, 1365
PspPI GGNCC 3 cut(s) 758, 1162, 1363
PspXI VCTCGAGB 1 cut(s) 330
PstNI CAGNNNCTG 1 cut(s) 836
PsuI RGATCY 1 cut(s) 912
PvuII CAGCTG 1 cut(s) 488
RsaI GTAC 1 cut(s) 938
RsaNI GTAC 1 cut(s) 937
RseI CAYNNNNRTG 1 cut(s) 714
SacI GAGCTC 2 cut(s) 32, 948
SaqAI TTAA 2 cut(s) 876, 996
SatI GCNGC 4 cut(s) 270, 489, 862, 952
Sau3AI GATC 4 cut(s) 566, 616, 912, 930
Sau96I GGNCC 3 cut(s) 758, 1162, 1363
ScaI AGTACT 1 cut(s) 938
SchI GAGTC 4 cut(s) 342, 842, 1099, 1318
ScrFI CCNGG 1 cut(s) 1156
SduI GDGCHC 3 cut(s) 32, 948, 1156
SfaNI GCATC 2 cut(s) 303, 1036
SfcI CTRYAG 1 cut(s) 642
Sfr274I CTCGAG 1 cut(s) 330
SinI GGWCC 2 cut(s) 758, 1162
SlaI CTCGAG 1 cut(s) 330
SmiMI CAYNNNNRTG 1 cut(s) 714
SmlI CTYRAG 3 cut(s) 330, 986, 1334
SmoI CTYRAG 3 cut(s) 330, 986, 1334
Sse9I AATT 3 cut(s) 545, 611, 1020
SsiI CCGC 2 cut(s) 465, 952
SspMI CTAG 5 cut(s) 278, 294, 354, 518, 1209
SstI GAGCTC 2 cut(s) 32, 948
StyD4I CCNGG 1 cut(s) 1154
StyI CCWWGG 3 cut(s) 353, 1248, 1367
TaaI ACNGT 3 cut(s) 886, 1033, 1197
TaiI ACGT 1 cut(s) 118
TaqI TCGA 1 cut(s) 331
TasI AATT 3 cut(s) 545, 611, 1020
TatI WGTACW 1 cut(s) 936
TauI GCSGC 1 cut(s) 954
TfiI GAWTC 1 cut(s) 633
Tru1I TTAA 2 cut(s) 876, 996
Tru9I TTAA 2 cut(s) 876, 996
TscAI CASTG 4 cut(s) 154, 242, 702, 895
TseFI GTSAC 2 cut(s) 318, 880
TseI GCWGC 3 cut(s) 269, 488, 861
Tsp45I GTSAC 2 cut(s) 318, 880
TspDTI ATGAA 6 cut(s) 176, 426, 597, 726, 962, 1148
TspGWI ACGGA 1 cut(s) 232
TspRI CASTG 4 cut(s) 154, 242, 702, 895
VpaK11BI GGWCC 2 cut(s) 758, 1162
XapI RAATTY 1 cut(s) 611
XceI RCATGY 3 cut(s) 192, 326, 660
XhoI CTCGAG 1 cut(s) 330
XmaJI CCTAGG 1 cut(s) 353
XmnI GAANNNNTTC 2 cut(s) 717, 1021
XspI CTAG 5 cut(s) 278, 294, 354, 518, 1209
ZrmI AGTACT 1 cut(s) 938
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.