RLG00000014652

B3 domain-containing protein Os01g0234100-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0001540
Physical Location & Seq
Forward (+)
55789722 .. 55792787
3066 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000014652

Sequence Viewer

Length: 1344 bp
ATGGCGATTGTTCAAGAACAGAAACAGAGCTCCAAAATTAACAGGGGAAGATCTCCAAACAAGGAAGAAAAGCTGTTGAAGAGAAAATCTTCGTGTCTCAAACAGACAACCAAGAACAATATGGTCAATGCTAAATCCATCAAGTCTGAAAGTCTTAAACCAAAGAGATTAAAGAGGGCGACAGAAGAAAGCGTATATGACACTTCCCAAGCTCAGCTCTCTGTTCTGGAAAGAGCAAAAGAGATTGAAGCAAGTTTAGCACCAGAGTTTCCTACACTGATCAAAGTTATGCTACCGTCACATGTTATGGGAGGATTCTGGCTTGGTCTTCCTAAGAAATTTTGTTGTGTGCATTTGCCAAAACATGACACGATGATTTCTTTGGAAGATGAAAGTGGGGAAGAATACCAAACCAAATATCTTGCGGACAAGGTTGGACTAAGTGGTGGATGGAGAGGCTTTTCGATTGCTCACAAGTTAGTGGAGGGAGATGTGGTAGTTTTCCATCGTGTCACTCCTTCAAAGTTCAAGGTGTATATTGTTCGATCAAAGGGTTCAGATGAATCGGATTGCTCTCTTGGCCTTATGAACCTAGGTGTTTGCATTAAAGAAATGGATACTGGGGACATTACTACTTGTGAAGGGGCAGAATATGAGGATATTAGACCTATTTCGGTGGAAAATCCTCAAAACTATAACACCATAGCCTGCAATACTAAATCCAGTCCGATTGCTGATCTGTCTGAAAATGATACTGGATGTCTTGGTTCTGAAGTGTTTGATGGAATTGGACTCGCAGAGTCTGTGATTCCTTTCAAAGAAGTGAGCTCCTTGGAGAACTTCAACTTCATCGTCGATGGTTTCATCATAAATTCTGAGTTTCCCAAACCAGTTTTGGCAAAGTACTATGACCTTTGCTGTAGTCAAAACACATACCTTCACGAACATCTTCTGGATGGTCTTAATTGGCAACTCATAACCGGGGCAATTTCTGAAATAACCACCATCGCAGAAGGCATAAGGACCTGCAAGGCCTCCACATTACAATGTAATTTTCCAACTTGGAATAAGACTCTACAGGCCTTTGAGGTGTTTGGCATGAACGTTGGTTTCCTGCGTACCCGGTTAGACAAGCTTGCAAGTCTCGCTTCAGAGTCAAAAAGAGGTAAAGAGGCAAGACATGAGAGGGAACAAGCAGAAGTAGAGATGAGAACCTTGGAGGCAAGGCTGTTGGAAGTGAAAAGGACCATAAGCAGGCTGTTGGAAGTAGAGACTGAGACTCAAAATGTGAGTTCAGAAAAGCTTGAGGATATGCTCCAACAAGTGGCTAAGGCCCCCTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

448

Amino Acids

50.08

Weight (kDa)

6.03

Isoelectric Point (pI)

39.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 94 - 182 9.7e-16 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1034
AccB7I CCANNNNNTGG 1 cut(s) 1324
AciI CCGC 1 cut(s) 425
AclI AACGTT 1 cut(s) 1104
AcsI RAATTY 2 cut(s) 338, 871
AcuI CTGAAG 2 cut(s) 792, 1134
AfaI GTAC 2 cut(s) 905, 1120
AfiI CCNNNNNNNGG 2 cut(s) 1254, 1324
AflIII ACRYGT 1 cut(s) 301
AgsI TTSAA 7 cut(s) 14, 79, 248, 522, 529, 817, 844
AjnI CCWGG 1 cut(s) 1337
AluBI AGCT 7 cut(s) 30, 73, 212, 217, 828, 1135, 1303
AluI AGCT 7 cut(s) 30, 73, 212, 217, 828, 1135, 1303
Alw21I GWGCWC 2 cut(s) 32, 830
Alw26I GTCTC 4 cut(s) 101, 1148, 1265, 1271
AlwNI CAGNNNCTG 1 cut(s) 803
AoxI GGCC 4 cut(s) 580, 1032, 1080, 1332
ApoI RAATTY 2 cut(s) 338, 871
Asp700I GAANNNNTTC 2 cut(s) 88, 948
AspA2I CCTAGG 1 cut(s) 592
AspS9I GGNCC 3 cut(s) 1023, 1245, 1333
AsuC2I CCSGG 2 cut(s) 982, 1123
AvaII GGWCC 2 cut(s) 1023, 1245
AvrII CCTAGG 1 cut(s) 592
BanII GRGCYC 2 cut(s) 32, 830
BbsI GAAGAC 1 cut(s) 320
Bbv12I GWGCWC 2 cut(s) 32, 830
BccI CCATC 7 cut(s) 146, 444, 513, 776, 851, 950, 1013
BciT130I CCWGG 1 cut(s) 1339
BciVI GTATCC 1 cut(s) 610
BclI TGATCA 1 cut(s) 279
BcnI CCSGG 2 cut(s) 982, 1123
BcoDI GTCTC 4 cut(s) 101, 1148, 1265, 1271
BfaI CTAG 1 cut(s) 593
BfmI CTRYAG 2 cut(s) 919, 1076
BfuAI ACCTGC 1 cut(s) 1034
BfuI GTATCC 1 cut(s) 610
BglII AGATCT 1 cut(s) 50
BlnI CCTAGG 1 cut(s) 592
BlpI GCTNAGC 1 cut(s) 213
BmcAI AGTACT 1 cut(s) 905
Bme1390I CCNGG 3 cut(s) 982, 1123, 1339
Bme18I GGWCC 2 cut(s) 1023, 1245
BmgT120I GGNCC 3 cut(s) 1023, 1245, 1333
BmiI GGNNCC 1 cut(s) 1335
BmrFI CCNGG 3 cut(s) 982, 1123, 1339
BmrI ACTGGG 1 cut(s) 630
BmuI ACTGGG 1 cut(s) 630
BpiI GAAGAC 1 cut(s) 320
Bpu10I CCTNAGC 1 cut(s) 1329
Bpu1102I GCTNAGC 1 cut(s) 213
BpuEI CTTGAG 1 cut(s) 1325
BpuMI CCSGG 2 cut(s) 982, 1123
BsaJI CCNNGG 5 cut(s) 592, 831, 981, 1215, 1337
BsaXI ACNNNNNCTCC 2 cut(s) 480, 510
Bsc4I CCNNNNNNNGG 2 cut(s) 1254, 1324
Bse1I ACTGG 4 cut(s) 625, 723, 760, 890
BseBI CCWGG 1 cut(s) 1339
BseDI CCNNGG 5 cut(s) 592, 831, 981, 1215, 1337
BseGI GGATG 3 cut(s) 455, 764, 961
BseLI CCNNNNNNNGG 2 cut(s) 1254, 1324
BseMII CTCAG 3 cut(s) 227, 867, 1266
BseNI ACTGG 4 cut(s) 625, 723, 760, 890
BshFI GGCC 4 cut(s) 582, 1034, 1082, 1334
BsiHKAI GWGCWC 2 cut(s) 32, 830
BsiSI CCGG 2 cut(s) 981, 1123
BslFI GGGAC 1 cut(s) 638
BslI CCNNNNNNNGG 2 cut(s) 1254, 1324
BsmAI GTCTC 4 cut(s) 101, 1148, 1265, 1271
BsmFI GGGAC 1 cut(s) 638
BsnI GGCC 4 cut(s) 582, 1034, 1082, 1334
Bsp1286I GDGCHC 2 cut(s) 32, 830
Bsp143I GATC 4 cut(s) 50, 279, 545, 736
Bsp1720I GCTNAGC 1 cut(s) 213
BspACI CCGC 1 cut(s) 425
BspANI GGCC 4 cut(s) 582, 1034, 1082, 1334
BspCNI CTCAG 3 cut(s) 226, 868, 1267
BspLI GGNNCC 1 cut(s) 1335
BspMI ACCTGC 1 cut(s) 1034
BsrI ACTGG 4 cut(s) 625, 723, 760, 890
BssECI CCNNGG 5 cut(s) 592, 831, 981, 1215, 1337
BssMI GATC 4 cut(s) 50, 279, 545, 736
BssT1I CCWWGG 3 cut(s) 592, 831, 1215
Bst2UI CCWGG 1 cut(s) 1339
Bst4CI ACNGT 1 cut(s) 297
Bst6I CTCTTC 1 cut(s) 74
BstC8I GCNNGC 3 cut(s) 709, 1137, 1256
BstDEI CTNAG 6 cut(s) 213, 333, 440, 876, 1275, 1329
BstF5I GGATG 3 cut(s) 455, 764, 961
BstKTI GATC 4 cut(s) 53, 282, 548, 739
BstMAI GTCTC 4 cut(s) 101, 1148, 1265, 1271
BstMBI GATC 4 cut(s) 50, 279, 545, 736
BstMWI GCNNNNNNNGC 3 cut(s) 257, 579, 1145
BstNI CCWGG 1 cut(s) 1339
BstNSI RCATGY 1 cut(s) 305
BstSCI CCNGG 3 cut(s) 980, 1121, 1337
BstSFI CTRYAG 2 cut(s) 919, 1076
BstV2I GAAGAC 1 cut(s) 320
BstX2I RGATCY 1 cut(s) 50
BstYI RGATCY 1 cut(s) 50
BsuI GTATCC 1 cut(s) 610
BsuRI GGCC 4 cut(s) 582, 1034, 1082, 1334
BtgZI GCGATG 1 cut(s) 991
BtsCI GGATG 3 cut(s) 455, 764, 961
BtsIMutI CAGTG 1 cut(s) 275
BveI ACCTGC 1 cut(s) 1034
Cac8I GCNNGC 3 cut(s) 709, 1137, 1256
CaiI CAGNNNCTG 1 cut(s) 803
Cfr13I GGNCC 3 cut(s) 1023, 1245, 1333
Csp6I GTAC 2 cut(s) 904, 1119
CviAII CATG 4 cut(s) 302, 365, 1099, 1181
CviQI GTAC 2 cut(s) 904, 1119
DdeI CTNAG 6 cut(s) 213, 333, 440, 876, 1275, 1329
DpnI GATC 4 cut(s) 52, 281, 547, 738
DpnII GATC 4 cut(s) 50, 279, 545, 736
Eam1104I CTCTTC 1 cut(s) 74
EarI CTCTTC 1 cut(s) 74
Ecl136II GAGCTC 2 cut(s) 30, 828
Eco130I CCWWGG 3 cut(s) 592, 831, 1215
Eco147I AGGCCT 2 cut(s) 1034, 1082
Eco24I GRGCYC 2 cut(s) 32, 830
Eco47I GGWCC 2 cut(s) 1023, 1245
Eco53kI GAGCTC 2 cut(s) 30, 828
Eco57I CTGAAG 2 cut(s) 792, 1134
EcoICRI GAGCTC 2 cut(s) 30, 828
EcoO109I RGGNCCY 2 cut(s) 1023, 1333
EcoRII CCWGG 1 cut(s) 1337
EcoT14I CCWWGG 3 cut(s) 592, 831, 1215
EcoT38I GRGCYC 2 cut(s) 32, 830
ErhI CCWWGG 3 cut(s) 592, 831, 1215
FaeI CATG 4 cut(s) 305, 368, 1102, 1184
FalI AAGNNNNNCTT 2 cut(s) 1132, 1164
FaqI GGGAC 1 cut(s) 638
FatI CATG 4 cut(s) 301, 364, 1098, 1180
FbaI TGATCA 1 cut(s) 279
FokI GGATG 3 cut(s) 462, 771, 968
FriOI GRGCYC 2 cut(s) 32, 830
FspBI CTAG 1 cut(s) 593
HaeIII GGCC 4 cut(s) 582, 1034, 1082, 1334
HapII CCGG 2 cut(s) 981, 1123
Hin1II CATG 4 cut(s) 305, 368, 1102, 1184
HindIII AAGCTT 2 cut(s) 1133, 1301
HinfI GANTC 8 cut(s) 315, 563, 792, 800, 808, 1072, 1154, 1279
HpaII CCGG 2 cut(s) 981, 1123
Hpy188III TCNNGA 4 cut(s) 14, 227, 941, 953
Hpy99I CGWCG 1 cut(s) 857
HpyAV CCTTC 4 cut(s) 528, 635, 947, 1007
HpyCH4III ACNGT 1 cut(s) 297
HpyCH4IV ACGT 1 cut(s) 1104
HpyCH4V TGCA 5 cut(s) 352, 603, 711, 1029, 1139
HpyF10VI GCNNNNNNNGC 3 cut(s) 257, 579, 1145
HpyF3I CTNAG 6 cut(s) 213, 333, 440, 876, 1275, 1329
HpySE526I ACGT 1 cut(s) 1104
Hsp92II CATG 4 cut(s) 305, 368, 1102, 1184
Ksp22I TGATCA 1 cut(s) 279
Kzo9I GATC 4 cut(s) 50, 279, 545, 736
LmnI GCTCC 3 cut(s) 35, 833, 1320
MaeI CTAG 1 cut(s) 593
MaeII ACGT 1 cut(s) 1104
MaeIII GTNAC 2 cut(s) 297, 511
MalI GATC 4 cut(s) 52, 281, 547, 738
MboI GATC 4 cut(s) 50, 279, 545, 736
MboII GAAGA 9 cut(s) 60, 77, 81, 91, 197, 320, 398, 413, 941
MflI RGATCY 1 cut(s) 50
MhlI GDGCHC 2 cut(s) 32, 830
MluCI AATT 7 cut(s) 36, 338, 786, 871, 964, 987, 1051
MlyI GAGTC 5 cut(s) 786, 809, 1066, 1163, 1273
MmeI TCCRAC 5 cut(s) 415, 1082, 1212, 1242, 1342
MroXI GAANNNNTTC 2 cut(s) 88, 948
MseI TTAA 5 cut(s) 39, 156, 170, 606, 963
MslI CAYNNNNRTG 1 cut(s) 1045
MspI CCGG 2 cut(s) 981, 1123
MspR9I CCNGG 3 cut(s) 982, 1123, 1339
MvaI CCWGG 1 cut(s) 1339
MwoI GCNNNNNNNGC 3 cut(s) 257, 579, 1145
NciI CCSGG 2 cut(s) 982, 1123
NdeII GATC 4 cut(s) 50, 279, 545, 736
NlaIII CATG 4 cut(s) 305, 368, 1102, 1184
NlaIV GGNNCC 1 cut(s) 1335
NmuCI GTSAC 2 cut(s) 297, 511
NspI RCATGY 1 cut(s) 305
PceI AGGCCT 2 cut(s) 1034, 1082
PciI ACATGT 1 cut(s) 301
PdmI GAANNNNTTC 2 cut(s) 88, 948
PfeI GAWTC 3 cut(s) 315, 563, 808
PflMI CCANNNNNTGG 1 cut(s) 1324
PleI GAGTC 5 cut(s) 786, 808, 1066, 1162, 1273
PpsI GAGTC 5 cut(s) 786, 808, 1066, 1162, 1273
PpuMI RGGWCCY 1 cut(s) 1023
PscI ACATGT 1 cut(s) 301
Psp124BI GAGCTC 2 cut(s) 32, 830
Psp1406I AACGTT 1 cut(s) 1104
Psp5II RGGWCCY 1 cut(s) 1023
Psp6I CCWGG 1 cut(s) 1337
PspGI CCWGG 1 cut(s) 1337
PspN4I GGNNCC 1 cut(s) 1335
PspPI GGNCC 3 cut(s) 1023, 1245, 1333
PspPPI RGGWCCY 1 cut(s) 1023
PstNI CAGNNNCTG 1 cut(s) 803
PsuI RGATCY 1 cut(s) 50
RsaI GTAC 2 cut(s) 905, 1120
RsaNI GTAC 2 cut(s) 904, 1119
RseI CAYNNNNRTG 1 cut(s) 1045
SacI GAGCTC 2 cut(s) 32, 830
SaqAI TTAA 5 cut(s) 39, 156, 170, 606, 963
Sau3AI GATC 4 cut(s) 50, 279, 545, 736
Sau96I GGNCC 3 cut(s) 1023, 1245, 1333
ScaI AGTACT 1 cut(s) 905
SchI GAGTC 5 cut(s) 786, 809, 1066, 1163, 1273
ScrFI CCNGG 3 cut(s) 982, 1123, 1339
SduI GDGCHC 2 cut(s) 32, 830
SfcI CTRYAG 2 cut(s) 919, 1076
SinI GGWCC 2 cut(s) 1023, 1245
SmiMI CAYNNNNRTG 1 cut(s) 1045
SmlI CTYRAG 1 cut(s) 1304
SmoI CTYRAG 1 cut(s) 1304
Sse9I AATT 7 cut(s) 36, 338, 786, 871, 964, 987, 1051
SseBI AGGCCT 2 cut(s) 1034, 1082
SsiI CCGC 1 cut(s) 425
SspMI CTAG 1 cut(s) 593
SstI GAGCTC 2 cut(s) 32, 830
StuI AGGCCT 2 cut(s) 1034, 1082
StyD4I CCNGG 3 cut(s) 980, 1121, 1337
StyI CCWWGG 3 cut(s) 592, 831, 1215
TaaI ACNGT 1 cut(s) 297
TaiI ACGT 1 cut(s) 1107
TaqI TCGA 3 cut(s) 464, 544, 855
TasI AATT 7 cut(s) 36, 338, 786, 871, 964, 987, 1051
TatI WGTACW 1 cut(s) 903
TfiI GAWTC 3 cut(s) 315, 563, 808
Tru1I TTAA 5 cut(s) 39, 156, 170, 606, 963
Tru9I TTAA 5 cut(s) 39, 156, 170, 606, 963
TscAI CASTG 1 cut(s) 282
TseFI GTSAC 2 cut(s) 297, 511
Tsp45I GTSAC 2 cut(s) 297, 511
TspDTI ATGAA 6 cut(s) 405, 576, 602, 838, 853, 1115
TspRI CASTG 1 cut(s) 282
Van91I CCANNNNNTGG 1 cut(s) 1324
VpaK11BI GGWCC 2 cut(s) 1023, 1245
XapI RAATTY 2 cut(s) 338, 871
XceI RCATGY 1 cut(s) 305
XcmI CCANNNNNNNNNTGG 2 cut(s) 118, 892
XmaJI CCTAGG 1 cut(s) 592
XmnI GAANNNNTTC 2 cut(s) 88, 948
XspI CTAG 1 cut(s) 593
ZrmI AGTACT 1 cut(s) 905
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.