MD05G1079100.v1.1

B3 domain-containing protein Os01g0234100-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0001540
Physical Location & Seq
Forward (+)
16667560 .. 16670584
3025 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1079100.v1.1.491

Sequence Viewer

Length: 1311 bp
ATGGAGGAAGCAATGGTTATTCTTCAAGACCAGAAACAGAGCTCCTCTTACCCACTACTACAGCTACCGCCGCCGCCTCCGCTGCATAAGGGCATGGAAACCGAAAAGCAGTTCGGGCAAAAACCTCCATGCTTTCAACCGACGACTACAAGCAATGTGTTGAATGCTAATGCTAATCCCATGATGTTTAAATGTCCTAAACGAAAGGCCCCTGTAAGAGTTAGAGTAGAAAGCTTATACGATAGTTCCCAGGCTCAGTCTTCTGTTTTGGAAAGAGCAAAGAAGATCGAAGCAAGCCTAGATGATAAGTTTCCTTCACTACTCAAAGTTATGATGCCGTCAGAAGTTTCGGGAGGATTCTACCTTAATCTTGCTAAGAAATTTCGCAGTGAATATATGCCAAAACAAGATTCAATGATTGTTTTGGAAGATGAAAATGGGAAGGAATTTGGAACGAAGTATCTTGTGGAGAAGGGTGGACTGAGTGGTGGATGGAGAGGGTTTTCAATTGCTCACAAGATAAAGAAGGGAGATGTTGTGATTTTCCATCTAGTCACTCCTTCCAAATTTAAGGTATACATCGTTAGGTCAAATGAAGTGGACTGGAGTATTCCTCTTATGAGGGTAGATCCTCCCATCAGACAGATGGATACCGGAGGCTTTACTGCATGTGGAAAGGAAGAACGTGAGAATTTGGAACCTAATCCGCAGGAGAATGCAATAGTCTGTTATACGGACTCTGGTCCAACATCAGATCAGTCTGGAAATGACGGCGAAGATGTTGGTTCCGAATTTCTGGATGGTCTTAGACTTTCAGAGTCTGTTGTCACTTTTAAAGAAGTGCACTGTTTGGACAACTTTAATGTTCAGGTCAATGGTTTAATCATAAACTCTGAATTTTCAAAATACGTCCTGACCAAGTATTATGAGCTTTGCTGCAGTCAGAAATCCTTCCTTCATGAACAACTTCTCGAGGGTCTGAACTGCAAACTGATTGCCGGAGTAATTTCTGAGACAGTCAACATTGCAGATGCCATCAGGGCCTGCAAGATCACTACCCCGGAAGATTATCTTTCGACTTGGGATAAGACTCTGAAAGCCTTTGGTGATTTAGGCATGAATGTCCGTTTCCTGCGTGCCCGCCTGGACCAGCTTACAAGTCTTGCTTCAAAATCAAAAAGACTTGAGGCAAATGTTTTGGAAGTGAAAGAGACCATGAATAGTCTTGACTCTGAGGTTGAGACTCCGAATACGAGTTTAGAGGAGGAGCGGGAAATTCCGTTCCAAGAAATGGCTAAAGCCCCTTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

437

Amino Acids

48.95

Weight (kDa)

5.39

Isoelectric Point (pI)

46.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 108 - 197 5.3e-14 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1291
AccBSI CCGCTC 1 cut(s) 1270
AccI GTMKAC 1 cut(s) 576
AciI CCGC 7 cut(s) 68, 71, 74, 80, 707, 1141, 1270
AclWI GGATC 1 cut(s) 623
AcsI RAATTY 7 cut(s) 380, 446, 566, 691, 791, 896, 1275
AfiI CCNNNNNNNGG 1 cut(s) 1291
AgsI TTSAA 7 cut(s) 26, 137, 163, 414, 507, 903, 1170
AjnI CCWGG 2 cut(s) 249, 1143
AjuI GAANNNNNNNTTGG 2 cut(s) 394, 426
AloI GAACNNNNNNTCC 2 cut(s) 1265, 1297
AluBI AGCT 5 cut(s) 42, 64, 234, 931, 1153
AluI AGCT 5 cut(s) 42, 64, 234, 931, 1153
Alw21I GWGCWC 2 cut(s) 44, 846
Alw26I GTCTC 3 cut(s) 1007, 1205, 1235
Alw44I GTGCAC 1 cut(s) 842
AlwI GGATC 1 cut(s) 623
AlwNI CAGNNNCTG 2 cut(s) 821, 1044
Ama87I CYCGRG 1 cut(s) 971
AoxI GGCC 2 cut(s) 207, 1041
ApaLI GTGCAC 1 cut(s) 842
ApeKI GCWGC 2 cut(s) 82, 936
ApoI RAATTY 7 cut(s) 380, 446, 566, 691, 791, 896, 1275
Asp700I GAANNNNTTC 2 cut(s) 950, 966
AspS9I GGNCC 4 cut(s) 208, 743, 1041, 1147
AsuC2I CCSGG 1 cut(s) 1061
AsuHPI GGTGA 1 cut(s) 1118
AvaI CYCGRG 1 cut(s) 971
AvaII GGWCC 2 cut(s) 743, 1147
BaeGI GKGCMC 2 cut(s) 846, 1141
BanII GRGCYC 1 cut(s) 44
BbsI GAAGAC 1 cut(s) 252
Bbv12I GWGCWC 2 cut(s) 44, 846
BbvI GCAGC 2 cut(s) 69, 923
BccI CCATC 6 cut(s) 486, 555, 640, 644, 794, 1043
BceAI ACGGC 2 cut(s) 322, 787
BciT130I CCWGG 2 cut(s) 251, 1145
BciVI GTATCC 1 cut(s) 643
BcnI CCSGG 1 cut(s) 1061
BcoDI GTCTC 3 cut(s) 1007, 1205, 1235
BfaI CTAG 2 cut(s) 299, 551
BfmI CTRYAG 2 cut(s) 59, 937
BfuI GTATCC 1 cut(s) 643
BglI GCCNNNNNGGC 1 cut(s) 1040
BisI GCNGC 4 cut(s) 71, 74, 83, 937
BlsI GCNGC 4 cut(s) 72, 75, 84, 938
Bme1390I CCNGG 3 cut(s) 251, 1061, 1145
Bme18I GGWCC 2 cut(s) 743, 1147
BmeT110I CYCGRG 1 cut(s) 971
BmgT120I GGNCC 4 cut(s) 208, 743, 1041, 1147
BmiI GGNNCC 3 cut(s) 210, 699, 787
BmrFI CCNGG 3 cut(s) 251, 1061, 1145
BmsI GCATC 2 cut(s) 324, 1021
BoxI GACNNNNGTC 1 cut(s) 741
BpiI GAAGAC 1 cut(s) 252
BplI GAGNNNNNCTC 2 cut(s) 598, 630
BpmI CTGGAG 1 cut(s) 625
BpuEI CTTGAG 1 cut(s) 1205
BpuMI CCSGG 1 cut(s) 1061
BsaI GGTCTC 1 cut(s) 1205
BsaJI CCNNGG 3 cut(s) 249, 1059, 1304
BsaWI WCCGGW 1 cut(s) 653
BsaXI ACNNNNNCTCC 2 cut(s) 522, 552
Bsc4I CCNNNNNNNGG 1 cut(s) 1291
Bse1I ACTGG 1 cut(s) 608
Bse3DI GCAATG 3 cut(s) 18, 160, 1023
BseBI CCWGG 2 cut(s) 251, 1145
BseDI CCNNGG 3 cut(s) 249, 1059, 1304
BseGI GGATG 2 cut(s) 497, 805
BseLI CCNNNNNNNGG 1 cut(s) 1291
BseMI GCAATG 3 cut(s) 18, 160, 1023
BseMII CTCAG 4 cut(s) 269, 473, 1002, 1224
BseNI ACTGG 1 cut(s) 608
BseRI GAGGAG 3 cut(s) 34, 1277, 1280
BseSI GKGCMC 2 cut(s) 846, 1141
BseXI GCAGC 2 cut(s) 69, 923
BshFI GGCC 2 cut(s) 209, 1043
BsiHKAI GWGCWC 2 cut(s) 44, 846
BsiHKCI CYCGRG 1 cut(s) 971
BsiSI CCGG 3 cut(s) 654, 999, 1061
BslI CCNNNNNNNGG 1 cut(s) 1291
BsmAI GTCTC 3 cut(s) 1007, 1205, 1235
BsmI GAATGC 2 cut(s) 169, 721
BsnI GGCC 2 cut(s) 209, 1043
Bso31I GGTCTC 1 cut(s) 1205
BsoBI CYCGRG 1 cut(s) 971
Bsp1286I GDGCHC 3 cut(s) 44, 846, 1141
Bsp143I GATC 4 cut(s) 285, 628, 754, 1050
BspACI CCGC 7 cut(s) 68, 71, 74, 80, 707, 1141, 1270
BspANI GGCC 2 cut(s) 209, 1043
BspCNI CTCAG 4 cut(s) 268, 474, 1003, 1225
BspHI TCATGA 1 cut(s) 958
BspLI GGNNCC 3 cut(s) 210, 699, 787
BspMAI CTGCAG 1 cut(s) 941
BspPI GGATC 1 cut(s) 623
BspTNI GGTCTC 1 cut(s) 1205
BsrBI CCGCTC 1 cut(s) 1270
BsrDI GCAATG 3 cut(s) 18, 160, 1023
BsrI ACTGG 1 cut(s) 608
BssECI CCNNGG 3 cut(s) 249, 1059, 1304
BssMI GATC 4 cut(s) 285, 628, 754, 1050
BssNAI GTATAC 1 cut(s) 577
BssT1I CCWWGG 1 cut(s) 1304
Bst1107I GTATAC 1 cut(s) 577
Bst2UI CCWGG 2 cut(s) 251, 1145
Bst4CI ACNGT 2 cut(s) 848, 1018
BstC8I GCNNGC 4 cut(s) 295, 1045, 1137, 1141
BstDEI CTNAG 6 cut(s) 255, 375, 482, 806, 1011, 1233
BstF5I GGATG 2 cut(s) 497, 805
BstKTI GATC 4 cut(s) 288, 631, 757, 1053
BstMAI GTCTC 3 cut(s) 1007, 1205, 1235
BstMBI GATC 4 cut(s) 285, 628, 754, 1050
BstMWI GCNNNNNNNGC 5 cut(s) 70, 79, 82, 115, 1040
BstNI CCWGG 2 cut(s) 251, 1145
BstNSI RCATGY 1 cut(s) 672
BstPAI GACNNNNGTC 1 cut(s) 741
BstSCI CCNGG 3 cut(s) 249, 1059, 1143
BstSFI CTRYAG 2 cut(s) 59, 937
BstSLI GKGCMC 2 cut(s) 846, 1141
BstV1I GCAGC 2 cut(s) 69, 923
BstV2I GAAGAC 1 cut(s) 252
BstX2I RGATCY 1 cut(s) 628
BstYI RGATCY 1 cut(s) 628
BstZ17I GTATAC 1 cut(s) 577
BsuI GTATCC 1 cut(s) 643
BsuRI GGCC 2 cut(s) 209, 1043
BtsCI GGATG 2 cut(s) 497, 805
BtsI GCAGTG 1 cut(s) 394
BtsIMutI CAGTG 2 cut(s) 394, 844
Cac8I GCNNGC 4 cut(s) 295, 1045, 1137, 1141
CaiI CAGNNNCTG 2 cut(s) 821, 1044
CciI TCATGA 1 cut(s) 958
Cfr13I GGNCC 4 cut(s) 208, 743, 1041, 1147
CspCI CAANNNNNGTGG 2 cut(s) 579, 614
CviAII CATG 7 cut(s) 94, 129, 181, 669, 959, 1117, 1216
DdeI CTNAG 6 cut(s) 255, 375, 482, 806, 1011, 1233
DpnI GATC 4 cut(s) 287, 630, 756, 1052
DpnII GATC 4 cut(s) 285, 628, 754, 1050
DraI TTTAAA 2 cut(s) 190, 835
Ecl136II GAGCTC 1 cut(s) 42
Eco130I CCWWGG 1 cut(s) 1304
Eco24I GRGCYC 1 cut(s) 44
Eco31I GGTCTC 1 cut(s) 1205
Eco47I GGWCC 2 cut(s) 743, 1147
Eco53kI GAGCTC 1 cut(s) 42
Eco88I CYCGRG 1 cut(s) 971
EcoICRI GAGCTC 1 cut(s) 42
EcoO109I RGGNCCY 2 cut(s) 208, 1041
EcoRII CCWGG 2 cut(s) 249, 1143
EcoT14I CCWWGG 1 cut(s) 1304
EcoT38I GRGCYC 1 cut(s) 44
ErhI CCWWGG 1 cut(s) 1304
FaeI CATG 7 cut(s) 97, 132, 184, 672, 962, 1120, 1219
FalI AAGNNNNNCTT 4 cut(s) 1056, 1088, 1150, 1182
FatI CATG 7 cut(s) 93, 128, 180, 668, 958, 1116, 1215
FauI CCCGC 2 cut(s) 1148, 1263
FblI GTMKAC 1 cut(s) 576
Fnu4HI GCNGC 4 cut(s) 71, 74, 83, 937
FokI GGATG 2 cut(s) 504, 812
FriOI GRGCYC 1 cut(s) 44
Fsp4HI GCNGC 4 cut(s) 71, 74, 83, 937
FspBI CTAG 2 cut(s) 299, 551
GluI GCNGC 4 cut(s) 71, 74, 83, 937
GsuI CTGGAG 1 cut(s) 625
HaeIII GGCC 2 cut(s) 209, 1043
HapII CCGG 3 cut(s) 654, 999, 1061
Hin1II CATG 7 cut(s) 97, 132, 184, 672, 962, 1120, 1219
HincII GTYRAC 1 cut(s) 1021
HindII GTYRAC 1 cut(s) 1021
HindIII AAGCTT 1 cut(s) 232
HinfI GANTC 7 cut(s) 357, 410, 737, 818, 1090, 1229, 1243
HpaII CCGG 3 cut(s) 654, 999, 1061
HphI GGTGA 1 cut(s) 1118
Hpy166II GTNNAC 5 cut(s) 479, 577, 601, 844, 1021
Hpy188III TCNNGA 8 cut(s) 26, 351, 762, 797, 913, 959, 971, 1226
Hpy8I GTNNAC 5 cut(s) 479, 577, 601, 844, 1021
Hpy99I CGWCG 1 cut(s) 145
HpyAV CCTTC 7 cut(s) 324, 436, 466, 520, 570, 961, 965
HpyCH4III ACNGT 2 cut(s) 848, 1018
HpyCH4IV ACGT 2 cut(s) 685, 909
HpyCH4V TGCA 8 cut(s) 85, 668, 719, 844, 939, 987, 1028, 1047
HpyF10VI GCNNNNNNNGC 5 cut(s) 70, 79, 82, 115, 1040
HpyF3I CTNAG 6 cut(s) 255, 375, 482, 806, 1011, 1233
HpySE526I ACGT 2 cut(s) 685, 909
Hsp92II CATG 7 cut(s) 97, 132, 184, 672, 962, 1120, 1219
Kzo9I GATC 4 cut(s) 285, 628, 754, 1050
LmnI GCTCC 2 cut(s) 47, 1267
Lsp1109I GCAGC 2 cut(s) 69, 923
LweI GCATC 2 cut(s) 324, 1021
MaeI CTAG 2 cut(s) 299, 551
MaeII ACGT 2 cut(s) 685, 909
MaeIII GTNAC 2 cut(s) 553, 826
MalI GATC 4 cut(s) 287, 630, 756, 1052
MbiI CCGCTC 1 cut(s) 1270
MboI GATC 4 cut(s) 285, 628, 754, 1050
MboII GAAGA 7 cut(s) 14, 252, 295, 440, 692, 788, 1076
MfeI CAATTG 1 cut(s) 507
MflI RGATCY 1 cut(s) 628
MhlI GDGCHC 3 cut(s) 44, 846, 1141
MluCI AATT 9 cut(s) 380, 446, 507, 566, 691, 791, 896, 1005, 1275
MlyI GAGTC 5 cut(s) 731, 827, 1084, 1223, 1237
MmeI TCCRAC 1 cut(s) 770
MroXI GAANNNNTTC 2 cut(s) 950, 966
MseI TTAA 6 cut(s) 189, 366, 570, 834, 861, 881
MspA1I CMGCKG 1 cut(s) 82
MspI CCGG 3 cut(s) 654, 999, 1061
MspR9I CCNGG 3 cut(s) 251, 1061, 1145
MunI CAATTG 1 cut(s) 507
Mva1269I GAATGC 2 cut(s) 169, 721
MvaI CCWGG 2 cut(s) 251, 1145
MwoI GCNNNNNNNGC 5 cut(s) 70, 79, 82, 115, 1040
NciI CCSGG 1 cut(s) 1061
NdeII GATC 4 cut(s) 285, 628, 754, 1050
NlaIII CATG 7 cut(s) 97, 132, 184, 672, 962, 1120, 1219
NlaIV GGNNCC 3 cut(s) 210, 699, 787
NmuCI GTSAC 2 cut(s) 553, 826
NspI RCATGY 1 cut(s) 672
PaeR7I CTCGAG 1 cut(s) 971
PagI TCATGA 1 cut(s) 958
PctI GAATGC 2 cut(s) 169, 721
PdmI GAANNNNTTC 2 cut(s) 950, 966
PfeI GAWTC 2 cut(s) 357, 410
PflMI CCANNNNNTGG 1 cut(s) 1291
PkrI GCNGC 4 cut(s) 72, 75, 84, 938
PleI GAGTC 5 cut(s) 731, 826, 1084, 1223, 1237
PpsI GAGTC 5 cut(s) 731, 826, 1084, 1223, 1237
PshAI GACNNNNGTC 1 cut(s) 741
Psp124BI GAGCTC 1 cut(s) 44
Psp6I CCWGG 2 cut(s) 249, 1143
PspGI CCWGG 2 cut(s) 249, 1143
PspN4I GGNNCC 3 cut(s) 210, 699, 787
PspPI GGNCC 4 cut(s) 208, 743, 1041, 1147
PstI CTGCAG 1 cut(s) 941
PstNI CAGNNNCTG 2 cut(s) 821, 1044
PsuI RGATCY 1 cut(s) 628
SacI GAGCTC 1 cut(s) 44
SaqAI TTAA 6 cut(s) 189, 366, 570, 834, 861, 881
SatI GCNGC 4 cut(s) 71, 74, 83, 937
Sau3AI GATC 4 cut(s) 285, 628, 754, 1050
Sau96I GGNCC 4 cut(s) 208, 743, 1041, 1147
SchI GAGTC 5 cut(s) 731, 827, 1084, 1223, 1237
ScrFI CCNGG 3 cut(s) 251, 1061, 1145
SduI GDGCHC 3 cut(s) 44, 846, 1141
SfaNI GCATC 2 cut(s) 324, 1021
SfcI CTRYAG 2 cut(s) 59, 937
Sfr274I CTCGAG 1 cut(s) 971
SinI GGWCC 2 cut(s) 743, 1147
SlaI CTCGAG 1 cut(s) 971
SmlI CTYRAG 2 cut(s) 971, 1184
SmoI CTYRAG 2 cut(s) 971, 1184
Sse9I AATT 9 cut(s) 380, 446, 507, 566, 691, 791, 896, 1005, 1275
SsiI CCGC 7 cut(s) 68, 71, 74, 80, 707, 1141, 1270
SspMI CTAG 2 cut(s) 299, 551
SstI GAGCTC 1 cut(s) 44
StyD4I CCNGG 3 cut(s) 249, 1059, 1143
StyI CCWWGG 1 cut(s) 1304
TaaI ACNGT 2 cut(s) 848, 1018
TaiI ACGT 2 cut(s) 688, 912
TaqI TCGA 3 cut(s) 288, 972, 1076
TasI AATT 9 cut(s) 380, 446, 507, 566, 691, 791, 896, 1005, 1275
TauI GCSGC 2 cut(s) 73, 76
TfiI GAWTC 2 cut(s) 357, 410
Tru1I TTAA 6 cut(s) 189, 366, 570, 834, 861, 881
Tru9I TTAA 6 cut(s) 189, 366, 570, 834, 861, 881
TscAI CASTG 2 cut(s) 394, 851
TseFI GTSAC 2 cut(s) 553, 826
TseI GCWGC 2 cut(s) 82, 936
Tsp45I GTSAC 2 cut(s) 553, 826
TspDTI ATGAA 6 cut(s) 447, 609, 947, 975, 1133, 1232
TspGWI ACGGA 3 cut(s) 749, 1115, 1269
TspRI CASTG 2 cut(s) 394, 851
Van91I CCANNNNNTGG 1 cut(s) 1291
VneI GTGCAC 1 cut(s) 842
VpaK11BI GGWCC 2 cut(s) 743, 1147
XapI RAATTY 7 cut(s) 380, 446, 566, 691, 791, 896, 1275
XceI RCATGY 1 cut(s) 672
XcmI CCANNNNNNNNNTGG 1 cut(s) 643
XhoI CTCGAG 1 cut(s) 971
XmiI GTMKAC 1 cut(s) 576
XmnI GAANNNNTTC 2 cut(s) 950, 966
XspI CTAG 2 cut(s) 299, 551
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.