Rorug05G0577300

B3 domain-containing protein Os01g0234100-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0001540
Physical Location & Seq
Forward (+)
77350654 .. 77354987
4334 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0577300.1

Sequence Viewer

Length: 2061 bp
ATGCCGAGGACCGCGAAACGCGAACGGAGCGCCGGTCAAGGCCGTGGCGTCGACGACGGCGAGGAGGCGGAGCGGCTCCGAGCCGTGAAGCGCGAGCGACTCAATTCCGAGCAGGTTGAAAATGAGTCAACCGATCAAGACGCCGGTCAACGGCGAGCTCTCCGATCTGACTACAATGAAATCCTGAACCTCATTAGTGAGAAGAGGAATGAGTTATTGAAGCCGGAGTCGGAGAAGTTTGGCGTCATCATCGGTAGAGTTGAGAAGTTGCATGAGCTAGTTCAGAAGCCAAGAGAGCAAGTGGCAGATGCAGAGGCACTGTTAGACATAACCAACACATTGGTGACCTCTGTCAAGTCACAGTCCAGTGCTGGCATTACCCCATCGGACTTCATTACCTGTTTGATTCGAGATTTTGGGCAATTAAGGGATGGCCATGCCTCGATTCGGTGGAAAGATGTTGGGGTTGCTGTGGCGCCAATTTTCAAGAGGGCTCATGGTTGCTGTACAATGCTTGGACCCATGAATACTGAGTATAAGGAAAGGAAGGCAGCTGTTCGTACTAGACGAATAAAGCCTACCACAACTGATGAGCCTGAGGAGATTGATGCACAGGAAGAAGAAAAAACTGATACGGATAGGAATATGTCAACGATGTTTGAGATATTAAGAAAGAAGAAACGTGTCCGACTGGAATGTTTAATATTAAACAGAATGTTCTTTGCACAGACAGTTGAGAATTTATTCGCTCTTTCGTTCCTTGTTAAAGATGGCCGGGCTGAAATCATAGTTGATGCAAATCGCGCTCATTTTGTTGCACCAAGGAATGCTGCCAAGGCCACTGAAGTGGCATCTCGTGAGGTTGTTTATAGACACTTTGTGTTCCGATTTGATTTCAAGGACTGGAAGTTGATGAAGGATATGGTGCCTGATGGTGAGGAGCTAATGCCACATAGAATCCCTGCCAACTCCGAACCTGCCTCTCAAGAGGAACCAGCTGCATACGTGTCCCAAACAGGACACACAACTCCAATTCGAAAGCTTTCCAGGAATCGCGGCCGGATGGTGCAGGAAGAAGCTATTGTAGAAGAATCCCCTGAATATGATAATGATCGTGCTGCTGGAGGCGCACTTCGCAGGGTAAGAAACTGCACTAACGCGGGAGAAGTTCAGCTGTTTTGTGATCAATCTGCTCCGAGAATCTCTATAATTCCAATGGCTGAGGAGTGTGCTGTCAATTTTGAGCTACTCAACTTCAGCCAAGACCCGGTGCCACGGCGAGAGCTCCGCTCCGACTACCTCGCCTTCCGGAGCTTCACCAGTGAGAAAAGAGACGATTTAATGAGTCCGGATTCGATGAAGTTTGATGTGATCATTGATATAGTTGACAAACTGCATGAGCAAGTTCAGCTTCCGAGAGAACAAGTAGCGGATGCACAAGCACTTCTGGACATAAGTAACACAGTGGTGGCCTCTGTTATGTCACATTCTAATGGGGAAACTACCCCCAGCGATTTCATTACTGGTTTGATTACATATTTCGGGCAAGTCAATAGGCCATCAGTGAACAAAGATAATGCCCATGTCTCACTAAAATGGAAAGATATTGGACTTTCTGTTTCACCAATACTCAAGAGGGCTGTTGGATGTTGCATAATGCTTGGACCCATGAATATTGAGATTAAGCAAAGGAAGGCTGCAGTCCGGCGTACAAAGCCTACCACAACTCATCTGCCCAAGGAGATCAATAATGAAGGAGGCGAAAGAACTGATACAGATTTGAACATGGCAACAATGTTTGAGATTTTAAAGAACAAGAAAAGGGTGGAATTCAGTTGTTTGATACTAAACAGAAGGTCTTTTGCACAGACAGTGGAGAATCTATTTGCTCTGTCATTTCTGGCAAAAGACGGCAGAGTTAGAATTGCTGTCGAAGCAAATGGTTCTCATATTGTTTCTCCTACGAATGGTCCTGTTGCCAATGAAGTGGCTTATCACCATTTTGTGTTCCGATTTGATTTCAAGGACTGGAAGATTGTTGGAATTGTATCAATGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

686

Amino Acids

77.65

Weight (kDa)

7.23

Isoelectric Point (pI)

49.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nse4_C PF08743 229 - 315 1.1e-19 Nse4 C-terminal
Nse4_C PF08743 609 - 678 2.5e-17 Nse4 C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 103, 985
AccB1I GGYRCC 3 cut(s) 475, 925, 1270
AccBSI CCGCTC 2 cut(s) 73, 1290
AccI GTMKAC 1 cut(s) 51
AccII CGCG 6 cut(s) 14, 21, 93, 804, 1056, 1160
AccIII TCCGGA 2 cut(s) 1308, 1348
AciI CCGC 7 cut(s) 12, 68, 73, 1056, 1160, 1288, 1430
AcoI YGGCCR 3 cut(s) 433, 772, 1057
AcsI RAATTY 2 cut(s) 739, 1829
AcuI CTGAAG 2 cut(s) 864, 1240
AcyI GRCGYC 4 cut(s) 48, 141, 243, 476
AdeI CACNNNGTG 1 cut(s) 880
AfaI GTAC 3 cut(s) 508, 562, 1711
AfiI CCNNNNNNNGG 4 cut(s) 150, 447, 1267, 1967
AflIII ACRYGT 2 cut(s) 682, 1005
AgsI TTSAA 6 cut(s) 119, 220, 487, 898, 1783, 2023
AjnI CCWGG 1 cut(s) 1046
AleI CACNNNNGTG 3 cut(s) 341, 845, 1466
Alw21I GWGCWC 2 cut(s) 160, 1287
Alw26I GTCTC 2 cut(s) 1326, 1591
Aor13HI TCCGGA 2 cut(s) 1308, 1348
AoxI GGCC 7 cut(s) 40, 433, 772, 837, 1057, 1470, 1556
ApeKI GCWGC 5 cut(s) 551, 830, 998, 1118, 1697
ApoI RAATTY 2 cut(s) 739, 1829
Asp700I GAANNNNTTC 2 cut(s) 743, 1042
AspLEI GCGC 5 cut(s) 32, 93, 478, 806, 1130
AspS9I GGNCC 4 cut(s) 9, 518, 1664, 1970
AsuC2I CCSGG 2 cut(s) 776, 1268
AsuHPI GGTGA 5 cut(s) 355, 947, 1309, 1614, 1988
AsuII TTCGAA 1 cut(s) 1036
AvaII GGWCC 4 cut(s) 9, 518, 1664, 1970
AxyI CCTNAGG 1 cut(s) 597
BalI TGGCCA 1 cut(s) 435
BanI GGYRCC 3 cut(s) 475, 925, 1270
BanII GRGCYC 3 cut(s) 160, 496, 1287
BauI CACGAG 1 cut(s) 855
Bbv12I GWGCWC 2 cut(s) 160, 1287
BbvCI CCTCAGC 1 cut(s) 1221
BbvI GCAGC 5 cut(s) 563, 817, 985, 1105, 1684
BccI CCATC 6 cut(s) 391, 425, 764, 926, 1057, 1567
BceAI ACGGC 6 cut(s) 27, 68, 73, 167, 1292, 1927
BciT130I CCWGG 1 cut(s) 1048
BclI TGATCA 2 cut(s) 1183, 1371
BcnI CCSGG 2 cut(s) 776, 1268
BcoDI GTCTC 2 cut(s) 1326, 1591
BfaI CTAG 2 cut(s) 278, 564
BfmI CTRYAG 1 cut(s) 1698
BfoI RGCGCY 2 cut(s) 33, 479
BfuAI ACCTGC 2 cut(s) 103, 985
BisI GCNGC 7 cut(s) 74, 552, 831, 999, 1057, 1119, 1698
BlsI GCNGC 7 cut(s) 75, 553, 832, 1000, 1058, 1120, 1699
Bme1390I CCNGG 3 cut(s) 776, 1048, 1268
Bme18I GGWCC 4 cut(s) 9, 518, 1664, 1970
BmgT120I GGNCC 4 cut(s) 9, 518, 1664, 1970
BmiI GGNNCC 7 cut(s) 77, 477, 520, 927, 993, 1272, 1666
BmrFI CCNGG 3 cut(s) 776, 1048, 1268
BmsI GCATC 5 cut(s) 298, 598, 784, 860, 1423
BoxI GACNNNNGTC 2 cut(s) 144, 350
BplI GAGNNNNNCTC 2 cut(s) 1274, 1306
BpmI CTGGAG 1 cut(s) 1143
Bpu10I CCTNAGC 1 cut(s) 1221
Bpu14I TTCGAA 1 cut(s) 1036
BpuEI CTTGAG 2 cut(s) 969, 1616
BpuMI CCSGG 2 cut(s) 776, 1268
BsaAI YACGTR 1 cut(s) 1006
BsaBI GATNNNNATC 2 cut(s) 798, 1110
BsaHI GRCGYC 4 cut(s) 48, 141, 243, 476
BsaJI CCNNGG 6 cut(s) 5, 43, 821, 834, 1274, 1737
BsaWI WCCGGW 2 cut(s) 1308, 1348
BsaXI ACNNNNNCTCC 2 cut(s) 19, 49
Bsc4I CCNNNNNNNGG 4 cut(s) 150, 447, 1267, 1967
Bse118I RCCGGY 2 cut(s) 32, 143
Bse1I ACTGG 6 cut(s) 366, 696, 908, 1320, 1528, 2033
Bse21I CCTNAGG 1 cut(s) 597
Bse8I GATNNNNATC 2 cut(s) 798, 1110
BseAI TCCGGA 2 cut(s) 1308, 1348
BseBI CCWGG 1 cut(s) 1048
BseDI CCNNGG 6 cut(s) 5, 43, 821, 834, 1274, 1737
BseGI GGATG 4 cut(s) 436, 1068, 1438, 1652
BseJI GATNNNNATC 2 cut(s) 798, 1110
BseLI CCNNNNNNNGG 4 cut(s) 150, 447, 1267, 1967
BseMII CTCAG 3 cut(s) 522, 588, 1212
BseNI ACTGG 6 cut(s) 366, 696, 908, 1320, 1528, 2033
BseRI GAGGAG 4 cut(s) 77, 614, 953, 1238
BseX3I CGGCCG 1 cut(s) 1057
BseXI GCAGC 5 cut(s) 563, 817, 985, 1105, 1684
BseYI CCCAGC 1 cut(s) 1508
BsgI GTGCAG 2 cut(s) 1088, 1135
Bsh1236I CGCG 6 cut(s) 14, 21, 93, 804, 1056, 1160
Bsh1285I CGRYCG 1 cut(s) 1060
BshFI GGCC 7 cut(s) 42, 435, 774, 839, 1059, 1472, 1558
BshNI GGYRCC 3 cut(s) 475, 925, 1270
BsiEI CGRYCG 1 cut(s) 1060
BsiHKAI GWGCWC 2 cut(s) 160, 1287
BsiSI CCGG 9 cut(s) 33, 144, 224, 775, 1060, 1268, 1309, 1349, 1705
BslFI GGGAC 1 cut(s) 994
BslI CCNNNNNNNGG 4 cut(s) 150, 447, 1267, 1967
BsmAI GTCTC 2 cut(s) 1326, 1591
BsmBI CGTCTC 1 cut(s) 1326
BsmFI GGGAC 1 cut(s) 994
BsmI GAATGC 1 cut(s) 832
BsnI GGCC 7 cut(s) 42, 435, 774, 839, 1059, 1472, 1558
Bsp119I TTCGAA 1 cut(s) 1036
Bsp1286I GDGCHC 3 cut(s) 160, 496, 1287
Bsp13I TCCGGA 2 cut(s) 1308, 1348
Bsp1407I TGTACA 1 cut(s) 506
Bsp143I GATC 6 cut(s) 133, 164, 1111, 1183, 1371, 1743
BspACI CCGC 7 cut(s) 12, 68, 73, 1056, 1160, 1288, 1430
BspANI GGCC 7 cut(s) 42, 435, 774, 839, 1059, 1472, 1558
BspCNI CTCAG 3 cut(s) 523, 589, 1213
BspEI TCCGGA 2 cut(s) 1308, 1348
BspFNI CGCG 6 cut(s) 14, 21, 93, 804, 1056, 1160
BspLI GGNNCC 7 cut(s) 77, 477, 520, 927, 993, 1272, 1666
BspMAI CTGCAG 1 cut(s) 1702
BspMI ACCTGC 2 cut(s) 103, 985
BspT104I TTCGAA 1 cut(s) 1036
BspT107I GGYRCC 3 cut(s) 475, 925, 1270
BsrBI CCGCTC 2 cut(s) 73, 1290
BsrFI RCCGGY 2 cut(s) 32, 143
BsrGI TGTACA 1 cut(s) 506
BsrI ACTGG 6 cut(s) 366, 696, 908, 1320, 1528, 2033
BssAI RCCGGY 2 cut(s) 32, 143
BssECI CCNNGG 6 cut(s) 5, 43, 821, 834, 1274, 1737
BssMI GATC 6 cut(s) 133, 164, 1111, 1183, 1371, 1743
BssNI GRCGYC 4 cut(s) 48, 141, 243, 476
BssSI CACGAG 1 cut(s) 855
BssT1I CCWWGG 3 cut(s) 821, 834, 1737
Bst2BI CACGAG 1 cut(s) 855
Bst2UI CCWGG 1 cut(s) 1048
Bst4CI ACNGT 5 cut(s) 321, 363, 733, 1465, 1873
Bst6I CTCTTC 1 cut(s) 197
BstACI GRCGYC 4 cut(s) 48, 141, 243, 476
BstAUI TGTACA 1 cut(s) 506
BstBAI YACGTR 1 cut(s) 1006
BstBI TTCGAA 1 cut(s) 1036
BstC8I GCNNGC 3 cut(s) 95, 156, 373
BstDEI CTNAG 3 cut(s) 531, 597, 1221
BstDSI CCRYGG 2 cut(s) 43, 1274
BstEII GGTNACC 1 cut(s) 343
BstF5I GGATG 4 cut(s) 436, 1068, 1438, 1652
BstFNI CGCG 6 cut(s) 14, 21, 93, 804, 1056, 1160
BstH2I RGCGCY 2 cut(s) 33, 479
BstHHI GCGC 5 cut(s) 32, 93, 478, 806, 1130
BstKTI GATC 6 cut(s) 136, 167, 1114, 1186, 1374, 1746
BstMAI GTCTC 2 cut(s) 1326, 1591
BstMBI GATC 6 cut(s) 133, 164, 1111, 1183, 1371, 1743
BstMCI CGRYCG 1 cut(s) 1060
BstMWI GCNNNNNNNGC 9 cut(s) 27, 295, 803, 836, 1127, 1134, 1408, 1714, 1934
BstNI CCWGG 1 cut(s) 1048
BstPAI GACNNNNGTC 2 cut(s) 144, 350
BstPI GGTNACC 1 cut(s) 343
BstSCI CCNGG 3 cut(s) 774, 1046, 1266
BstSFI CTRYAG 1 cut(s) 1698
BstUI CGCG 6 cut(s) 14, 21, 93, 804, 1056, 1160
BstV1I GCAGC 5 cut(s) 563, 817, 985, 1105, 1684
BstXI CCANNNNNNTGG 3 cut(s) 340, 847, 1987
BstZI CGGCCG 1 cut(s) 1057
Bsu36I CCTNAGG 1 cut(s) 597
BsuRI GGCC 7 cut(s) 42, 435, 774, 839, 1059, 1472, 1558
BtgI CCRYGG 2 cut(s) 43, 1274
BtsCI GGATG 4 cut(s) 436, 1068, 1438, 1652
BtsIMutI CAGTG 7 cut(s) 317, 373, 840, 1327, 1470, 1569, 1878
BveI ACCTGC 2 cut(s) 103, 985
Cac8I GCNNGC 3 cut(s) 95, 156, 373
CfoI GCGC 5 cut(s) 32, 93, 478, 806, 1130
Cfr10I RCCGGY 2 cut(s) 32, 143
Cfr13I GGNCC 4 cut(s) 9, 518, 1664, 1970
CseI GACGC 3 cut(s) 37, 149, 232
Csp6I GTAC 3 cut(s) 507, 561, 1710
CviAII CATG 8 cut(s) 272, 437, 497, 523, 1397, 1583, 1669, 1786
CviQI GTAC 3 cut(s) 507, 561, 1710
DdeI CTNAG 3 cut(s) 531, 597, 1221
DinI GGCGCC 1 cut(s) 477
DpnI GATC 6 cut(s) 135, 166, 1113, 1185, 1373, 1745
DpnII GATC 6 cut(s) 133, 164, 1111, 1183, 1371, 1743
DraI TTTAAA 1 cut(s) 1809
DraIII CACNNNGTG 1 cut(s) 880
EaeI YGGCCR 3 cut(s) 433, 772, 1057
EagI CGGCCG 1 cut(s) 1057
Eam1104I CTCTTC 1 cut(s) 197
EarI CTCTTC 1 cut(s) 197
EciI GGCGGA 1 cut(s) 83
Ecl136II GAGCTC 2 cut(s) 158, 1285
EclXI CGGCCG 1 cut(s) 1057
Eco130I CCWWGG 3 cut(s) 821, 834, 1737
Eco24I GRGCYC 3 cut(s) 160, 496, 1287
Eco47I GGWCC 4 cut(s) 9, 518, 1664, 1970
Eco52I CGGCCG 1 cut(s) 1057
Eco53kI GAGCTC 2 cut(s) 158, 1285
Eco57I CTGAAG 2 cut(s) 864, 1240
Eco81I CCTNAGG 1 cut(s) 597
Eco91I GGTNACC 1 cut(s) 343
EcoICRI GAGCTC 2 cut(s) 158, 1285
EcoO65I GGTNACC 1 cut(s) 343
EcoRI GAATTC 1 cut(s) 1829
EcoRII CCWGG 1 cut(s) 1046
EcoT14I CCWWGG 3 cut(s) 821, 834, 1737
EcoT38I GRGCYC 3 cut(s) 160, 496, 1287
EgeI GGCGCC 1 cut(s) 477
EheI GGCGCC 1 cut(s) 477
ErhI CCWWGG 3 cut(s) 821, 834, 1737
Esp3I CGTCTC 1 cut(s) 1326
FaeI CATG 8 cut(s) 275, 440, 500, 526, 1400, 1586, 1672, 1789
FalI AAGNNNNNCTT 2 cut(s) 1395, 1427
FaqI GGGAC 1 cut(s) 994
FatI CATG 8 cut(s) 271, 436, 496, 522, 1396, 1582, 1668, 1785
FauI CCCGC 1 cut(s) 1153
FbaI TGATCA 2 cut(s) 1183, 1371
FblI GTMKAC 1 cut(s) 51
Fnu4HI GCNGC 7 cut(s) 74, 552, 831, 999, 1057, 1119, 1698
FokI GGATG 4 cut(s) 443, 1075, 1445, 1659
FriOI GRGCYC 3 cut(s) 160, 496, 1287
Fsp4HI GCNGC 7 cut(s) 74, 552, 831, 999, 1057, 1119, 1698
FspBI CTAG 2 cut(s) 278, 564
GlaI GCGC 5 cut(s) 31, 92, 477, 805, 1129
GluI GCNGC 7 cut(s) 74, 552, 831, 999, 1057, 1119, 1698
GsaI CCCAGC 1 cut(s) 1512
GsuI CTGGAG 1 cut(s) 1143
HaeII RGCGCY 2 cut(s) 33, 479
HaeIII GGCC 7 cut(s) 42, 435, 774, 839, 1059, 1472, 1558
HapII CCGG 9 cut(s) 33, 144, 224, 775, 1060, 1268, 1309, 1349, 1705
HgaI GACGC 3 cut(s) 37, 149, 232
HhaI GCGC 5 cut(s) 32, 93, 478, 806, 1130
Hin1I GRCGYC 4 cut(s) 48, 141, 243, 476
Hin1II CATG 8 cut(s) 275, 440, 500, 526, 1400, 1586, 1672, 1789
Hin6I GCGC 5 cut(s) 30, 91, 476, 804, 1128
HinP1I GCGC 5 cut(s) 30, 91, 476, 804, 1128
HincII GTYRAC 5 cut(s) 52, 129, 149, 651, 1387
HindII GTYRAC 5 cut(s) 52, 129, 149, 651, 1387
HindIII AAGCTT 1 cut(s) 1040
HpaII CCGG 9 cut(s) 33, 144, 224, 775, 1060, 1268, 1309, 1349, 1705
HphI GGTGA 5 cut(s) 355, 947, 1309, 1614, 1988
Hpy166II GTNNAC 6 cut(s) 52, 129, 149, 651, 1387, 1567
Hpy8I GTNNAC 6 cut(s) 52, 129, 149, 651, 1387, 1567
Hpy99I CGWCG 3 cut(s) 53, 56, 59
HpyAV CCTTC 6 cut(s) 541, 910, 1315, 1687, 1748, 1848
HpyCH4III ACNGT 5 cut(s) 321, 363, 733, 1465, 1873
HpyCH4IV ACGT 2 cut(s) 682, 1005
HpyF10VI GCNNNNNNNGC 9 cut(s) 27, 295, 803, 836, 1127, 1134, 1408, 1714, 1934
HpyF3I CTNAG 3 cut(s) 531, 597, 1221
HpySE526I ACGT 2 cut(s) 682, 1005
Hsp92I GRCGYC 4 cut(s) 48, 141, 243, 476
Hsp92II CATG 8 cut(s) 275, 440, 500, 526, 1400, 1586, 1672, 1789
HspAI GCGC 5 cut(s) 30, 91, 476, 804, 1128
KasI GGCGCC 1 cut(s) 475
Kpn2I TCCGGA 2 cut(s) 1308, 1348
Ksp22I TGATCA 2 cut(s) 1183, 1371
Kzo9I GATC 6 cut(s) 133, 164, 1111, 1183, 1371, 1743
LmnI GCTCC 8 cut(s) 27, 70, 81, 940, 1198, 1290, 1295, 1311
Lsp1109I GCAGC 5 cut(s) 563, 817, 985, 1105, 1684
LweI GCATC 5 cut(s) 298, 598, 784, 860, 1423
MaeI CTAG 2 cut(s) 278, 564
MaeII ACGT 2 cut(s) 682, 1005
MaeIII GTNAC 4 cut(s) 343, 357, 1457, 1482
MalI GATC 6 cut(s) 135, 166, 1113, 1185, 1373, 1745
MbiI CCGCTC 2 cut(s) 73, 1290
MboI GATC 6 cut(s) 133, 164, 1111, 1183, 1371, 1743
MboII GAAGA 7 cut(s) 214, 629, 632, 688, 1085, 1100, 2044
MhlI GDGCHC 3 cut(s) 160, 496, 1287
MlsI TGGCCA 1 cut(s) 435
MluNI TGGCCA 1 cut(s) 435
Mly113I GGCGCC 1 cut(s) 476
MlyI GAGTC 4 cut(s) 93, 134, 236, 1354
MmeI TCCRAC 5 cut(s) 210, 712, 1317, 1624, 2020
Mox20I TGGCCA 1 cut(s) 435
MroI TCCGGA 2 cut(s) 1308, 1348
MroXI GAANNNNTTC 2 cut(s) 743, 1042
MscI TGGCCA 1 cut(s) 435
MseI TTAA 8 cut(s) 425, 668, 701, 707, 765, 1340, 1683, 1808
MslI CAYNNNNRTG 5 cut(s) 341, 845, 1466, 1491, 1594
Msp20I TGGCCA 1 cut(s) 435
MspA1I CMGCKG 3 cut(s) 554, 998, 1174
MspI CCGG 9 cut(s) 33, 144, 224, 775, 1060, 1268, 1309, 1349, 1705
MspR9I CCNGG 3 cut(s) 776, 1048, 1268
Mva1269I GAATGC 1 cut(s) 832
MvaI CCWGG 1 cut(s) 1048
MvnI CGCG 6 cut(s) 14, 21, 93, 804, 1056, 1160
MwoI GCNNNNNNNGC 9 cut(s) 27, 295, 803, 836, 1127, 1134, 1408, 1714, 1934
NarI GGCGCC 1 cut(s) 476
NciI CCSGG 2 cut(s) 776, 1268
NdeII GATC 6 cut(s) 133, 164, 1111, 1183, 1371, 1743
NlaIII CATG 8 cut(s) 275, 440, 500, 526, 1400, 1586, 1672, 1789
NlaIV GGNNCC 7 cut(s) 77, 477, 520, 927, 993, 1272, 1666
NmeAIII GCCGAG 1 cut(s) 30
NmuCI GTSAC 3 cut(s) 343, 357, 1482
NspV TTCGAA 1 cut(s) 1036
OliI CACNNNNGTG 3 cut(s) 341, 845, 1466
PcsI WCGNNNNNNNCGW 2 cut(s) 57, 565
PctI GAATGC 1 cut(s) 832
PdmI GAANNNNTTC 2 cut(s) 743, 1042
PfeI GAWTC 8 cut(s) 406, 445, 957, 1051, 1091, 1200, 1352, 1879
PfoI TCCNGGA 1 cut(s) 1046
PkrI GCNGC 7 cut(s) 75, 553, 832, 1000, 1058, 1120, 1699
PleI GAGTC 4 cut(s) 93, 133, 235, 1353
PluTI GGCGCC 1 cut(s) 479
PpsI GAGTC 4 cut(s) 93, 133, 235, 1353
Ppu21I YACGTR 1 cut(s) 1006
PshAI GACNNNNGTC 2 cut(s) 144, 350
Psp124BI GAGCTC 2 cut(s) 160, 1287
Psp6I CCWGG 1 cut(s) 1046
PspEI GGTNACC 1 cut(s) 343
PspFI CCCAGC 1 cut(s) 1508
PspGI CCWGG 1 cut(s) 1046
PspN4I GGNNCC 7 cut(s) 77, 477, 520, 927, 993, 1272, 1666
PspPI GGNCC 4 cut(s) 9, 518, 1664, 1970
PstI CTGCAG 1 cut(s) 1702
PvuII CAGCTG 3 cut(s) 554, 998, 1174
RsaI GTAC 3 cut(s) 508, 562, 1711
RsaNI GTAC 3 cut(s) 507, 561, 1710
RseI CAYNNNNRTG 5 cut(s) 341, 845, 1466, 1491, 1594
SacI GAGCTC 2 cut(s) 160, 1287
SalI GTCGAC 1 cut(s) 50
SaqAI TTAA 8 cut(s) 425, 668, 701, 707, 765, 1340, 1683, 1808
SatI GCNGC 7 cut(s) 74, 552, 831, 999, 1057, 1119, 1698
Sau3AI GATC 6 cut(s) 133, 164, 1111, 1183, 1371, 1743
Sau96I GGNCC 4 cut(s) 9, 518, 1664, 1970
SchI GAGTC 4 cut(s) 93, 134, 236, 1354
ScrFI CCNGG 3 cut(s) 776, 1048, 1268
SduI GDGCHC 3 cut(s) 160, 496, 1287
SfaNI GCATC 5 cut(s) 298, 598, 784, 860, 1423
SfcI CTRYAG 1 cut(s) 1698
SfoI GGCGCC 1 cut(s) 477
SfuI TTCGAA 1 cut(s) 1036
SgrDI CGTCGACG 1 cut(s) 50
SinI GGWCC 4 cut(s) 9, 518, 1664, 1970
SmiMI CAYNNNNRTG 5 cut(s) 341, 845, 1466, 1491, 1594
SmlI CTYRAG 2 cut(s) 984, 1631
SmoI CTYRAG 2 cut(s) 984, 1631
SsiI CCGC 7 cut(s) 12, 68, 73, 1056, 1160, 1288, 1430
SspDI GGCGCC 1 cut(s) 475
SspI AATATT 2 cut(s) 705, 1675
SspMI CTAG 2 cut(s) 278, 564
SstI GAGCTC 2 cut(s) 160, 1287
StyD4I CCNGG 3 cut(s) 774, 1046, 1266
StyI CCWWGG 3 cut(s) 821, 834, 1737
TaaI ACNGT 5 cut(s) 321, 363, 733, 1465, 1873
TaiI ACGT 2 cut(s) 685, 1008
TaqI TCGA 6 cut(s) 51, 409, 443, 1036, 1355, 1932
TatI WGTACW 1 cut(s) 506
TauI GCSGC 2 cut(s) 76, 1059
TfiI GAWTC 8 cut(s) 406, 445, 957, 1051, 1091, 1200, 1352, 1879
Tru1I TTAA 8 cut(s) 425, 668, 701, 707, 765, 1340, 1683, 1808
Tru9I TTAA 8 cut(s) 425, 668, 701, 707, 765, 1340, 1683, 1808
TscAI CASTG 7 cut(s) 324, 373, 847, 1327, 1470, 1569, 1878
TseFI GTSAC 3 cut(s) 343, 357, 1482
TseI GCWGC 5 cut(s) 551, 830, 998, 1118, 1697
Tsp45I GTSAC 3 cut(s) 343, 357, 1482
TspDTI ATGAA 9 cut(s) 192, 382, 539, 929, 1373, 1507, 1685, 1767, 1998
TspGWI ACGGA 2 cut(s) 40, 650
TspRI CASTG 7 cut(s) 324, 373, 847, 1327, 1470, 1569, 1878
VpaK11BI GGWCC 4 cut(s) 9, 518, 1664, 1970
XapI RAATTY 2 cut(s) 739, 1829
XmiI GTMKAC 1 cut(s) 51
XmnI GAANNNNTTC 2 cut(s) 743, 1042
XspI CTAG 2 cut(s) 278, 564
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.