MD10G1093200.v1.1

B3 domain-containing protein Os01g0234100-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0001540
Physical Location & Seq
Forward (+)
14336528 .. 14338584
2057 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1093200.v1.1.491

Sequence Viewer

Length: 1188 bp
ATGCAGGTGGTCAGTGCCAATCCTAAAAGGTCAGTGAGAGCTACAGTAGATAGCTTGTATGGTACTTGCCAAGCACCGTCTTCTGTTCTGGAAAGAGCCAAGGAGGTGGAGGCAACCCTAGATCCTAGGTTTCCTGTCCTGCTCAAAGTTATGCTGCCTTCACATGTTACCGGAGGATTCTGGCTTGGTCTTCCTAAGAAGTTCTGCTGTGAGAATTTGCCAAAACAAGATACAATGATTGTTTTGGAGGACGAAAGTGGGGAGGAGTTCCACACGAAATATCTTGTGGAGAAGGTGGGACTGAGTGGTGGATGGAGAGGGTTTTCGATTGCTCACAACCTGCTGGAGGGAGATGTTGTCGTCTTCCATGTACTCGCCCCTTCCAAGCTTAAGGTGTACATTGTCAGATCAAATGGTTCAGATGATTTGGACGGTGCTCTTGGCCTGATGAGATTAGATGCTTGCACTAAACAGATGGATACTGGAGACATTATTGCATGTGAAGGGGAAGATAGTGAGAATTTGGAATCTATTCCTGATGACAATGCAATAGCCTTTGATACAAAATCTGGTCTGTCATCAGACCATTCTGGAAATGATAGTGAAAATCTCGATTTTGAAGTTCTGGATGGACTTAGACTTTCAGAAACTGTTGTCACTTTTGAAGAAGTGAACTGCATGGAGAACTTCAACGTTCTCGTCAATGGTTTAAACATAAACTCAAAGTTTTCGAAACACATTCTGAGAAAGTACTATGAGCTTTGCTGCAGTCAGAACGCCTTCCTTCATGAACAACTTATCGAGGGTCTGAACTGCAAACTGGTTGCTGGAGTAATTTCTGAGACAGTCAACATTGCAGATGCCATCAGGGCCTGCAAAATAACTACCTCGAAAGGTAACTTTTCCACTTGGGATACGACTTTGAAAGCCTTTGACGATTTAGGCATGAATGTTGGGTTCCTGTCTGCCCGGCTGGACCATCTCACAAGTCTTGGTTTAAAATCAAAAAGATTTAAAGAGGCTAAACTTGAAAAGGACCGAGCGGAAGAAGAGAAGAGAAACTTGGAGGCAAAGCTTACTGAAGTTCAGAACGCAATTATTAGGCTCGCCTCTGAGATGAAGACTCTAAATACGAGTTCAGAGAAGCTTGAGCTTATGTTTCAAGAGGTGGCTAAAGCCCCTTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

396

Amino Acids

43.75

Weight (kDa)

5.07

Isoelectric Point (pI)

35.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 48 - 137 8.5e-16 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 348
AccBSI CCGCTC 1 cut(s) 1043
AciI CCGC 1 cut(s) 1043
AclI AACGTT 1 cut(s) 693
AclWI GGATC 1 cut(s) 116
AcsI RAATTY 2 cut(s) 214, 520
AcuI CTGAAG 1 cut(s) 1101
AfaI GTAC 4 cut(s) 64, 372, 398, 752
AfiI CCNNNNNNNGG 1 cut(s) 346
AflII CTTAAG 1 cut(s) 389
AflIII ACRYGT 1 cut(s) 163
AgsI TTSAA 6 cut(s) 620, 665, 691, 925, 1031, 1163
AjuI GAANNNNNNNTTGG 2 cut(s) 1046, 1078
AluBI AGCT 7 cut(s) 41, 54, 388, 760, 1075, 1147, 1153
AluI AGCT 7 cut(s) 41, 54, 388, 760, 1075, 1147, 1153
Alw21I GWGCWC 1 cut(s) 439
Alw26I GTCTC 2 cut(s) 480, 836
AlwI GGATC 1 cut(s) 116
AlwNI CAGNNNCTG 2 cut(s) 650, 873
AoxI GGCC 2 cut(s) 442, 870
ApeKI GCWGC 2 cut(s) 154, 765
ApoI RAATTY 2 cut(s) 214, 520
Asp700I GAANNNNTTC 2 cut(s) 531, 779
AspA2I CCTAGG 1 cut(s) 125
AspS9I GGNCC 3 cut(s) 870, 976, 1036
AsuC2I CCSGG 1 cut(s) 970
AsuII TTCGAA 1 cut(s) 731
AvaII GGWCC 2 cut(s) 976, 1036
AvrII CCTAGG 1 cut(s) 125
BbsI GAAGAC 4 cut(s) 72, 182, 355, 1127
Bbv12I GWGCWC 1 cut(s) 439
BbvI GCAGC 2 cut(s) 141, 752
BccI CCATC 5 cut(s) 306, 469, 623, 872, 987
BciVI GTATCC 2 cut(s) 472, 907
BcnI CCSGG 1 cut(s) 970
BcoDI GTCTC 2 cut(s) 480, 836
BfaI CTAG 2 cut(s) 119, 126
BfmI CTRYAG 2 cut(s) 42, 766
BfrI CTTAAG 1 cut(s) 389
BfuAI ACCTGC 1 cut(s) 348
BfuI GTATCC 2 cut(s) 472, 907
BglI GCCNNNNNGGC 1 cut(s) 869
BisI GCNGC 2 cut(s) 155, 766
BlnI CCTAGG 1 cut(s) 125
BlsI GCNGC 2 cut(s) 156, 767
BmcAI AGTACT 1 cut(s) 752
Bme1390I CCNGG 1 cut(s) 970
Bme18I GGWCC 2 cut(s) 976, 1036
BmgT120I GGNCC 3 cut(s) 870, 976, 1036
BmiI GGNNCC 1 cut(s) 959
BmrFI CCNGG 1 cut(s) 970
BmsI GCATC 2 cut(s) 448, 850
BpiI GAAGAC 4 cut(s) 72, 182, 355, 1127
BpmI CTGGAG 3 cut(s) 365, 504, 849
Bpu14I TTCGAA 1 cut(s) 731
BpuEI CTTGAG 1 cut(s) 1169
BpuMI CCSGG 1 cut(s) 970
BsaJI CCNNGG 3 cut(s) 99, 125, 1181
BsaWI WCCGGW 1 cut(s) 170
BsaXI ACNNNNNCTCC 2 cut(s) 342, 372
Bsc4I CCNNNNNNNGG 1 cut(s) 346
Bse1I ACTGG 2 cut(s) 487, 825
Bse3DI GCAATG 1 cut(s) 852
BseDI CCNNGG 3 cut(s) 99, 125, 1181
BseGI GGATG 2 cut(s) 317, 634
BseLI CCNNNNNNNGG 1 cut(s) 346
BseMI GCAATG 1 cut(s) 852
BseMII CTCAG 4 cut(s) 293, 734, 831, 1104
BseNI ACTGG 2 cut(s) 487, 825
BseRI GAGGAG 1 cut(s) 278
BseXI GCAGC 2 cut(s) 141, 752
BshFI GGCC 2 cut(s) 444, 872
BsiHKAI GWGCWC 1 cut(s) 439
BsiSI CCGG 2 cut(s) 171, 970
BslFI GGGAC 1 cut(s) 312
BslI CCNNNNNNNGG 1 cut(s) 346
BsmAI GTCTC 2 cut(s) 480, 836
BsmFI GGGAC 1 cut(s) 312
BsnI GGCC 2 cut(s) 444, 872
Bsp119I TTCGAA 1 cut(s) 731
Bsp1286I GDGCHC 1 cut(s) 439
Bsp1407I TGTACA 1 cut(s) 396
Bsp143I GATC 2 cut(s) 121, 407
BspACI CCGC 1 cut(s) 1043
BspANI GGCC 2 cut(s) 444, 872
BspCNI CTCAG 4 cut(s) 294, 735, 832, 1105
BspHI TCATGA 1 cut(s) 787
BspLI GGNNCC 1 cut(s) 959
BspMAI CTGCAG 1 cut(s) 770
BspMI ACCTGC 1 cut(s) 348
BspPI GGATC 1 cut(s) 116
BspT104I TTCGAA 1 cut(s) 731
BspTI CTTAAG 1 cut(s) 389
BsrBI CCGCTC 1 cut(s) 1043
BsrDI GCAATG 1 cut(s) 852
BsrGI TGTACA 1 cut(s) 396
BsrI ACTGG 2 cut(s) 487, 825
BssECI CCNNGG 3 cut(s) 99, 125, 1181
BssMI GATC 2 cut(s) 121, 407
BssT1I CCWWGG 3 cut(s) 99, 125, 1181
Bst4CI ACNGT 5 cut(s) 46, 78, 434, 652, 847
Bst6I CTCTTC 2 cut(s) 1044, 1049
BstAFI CTTAAG 1 cut(s) 389
BstAUI TGTACA 1 cut(s) 396
BstBI TTCGAA 1 cut(s) 731
BstC8I GCNNGC 3 cut(s) 463, 874, 1107
BstDEI CTNAG 6 cut(s) 195, 302, 635, 743, 840, 1113
BstENI CCTNNNNNAGG 1 cut(s) 344
BstF5I GGATG 2 cut(s) 317, 634
BstKTI GATC 2 cut(s) 124, 410
BstMAI GTCTC 2 cut(s) 480, 836
BstMBI GATC 2 cut(s) 121, 407
BstMWI GCNNNNNNNGC 1 cut(s) 869
BstNSI RCATGY 2 cut(s) 167, 501
BstSCI CCNGG 1 cut(s) 968
BstSFI CTRYAG 2 cut(s) 42, 766
BstV1I GCAGC 2 cut(s) 141, 752
BstV2I GAAGAC 4 cut(s) 72, 182, 355, 1127
BstX2I RGATCY 1 cut(s) 121
BstXI CCANNNNNNTGG 1 cut(s) 106
BstYI RGATCY 1 cut(s) 121
BsuI GTATCC 2 cut(s) 472, 907
BsuRI GGCC 2 cut(s) 444, 872
BtsCI GGATG 2 cut(s) 317, 634
BtsIMutI CAGTG 2 cut(s) 19, 39
BveI ACCTGC 1 cut(s) 348
Cac8I GCNNGC 3 cut(s) 463, 874, 1107
CaiI CAGNNNCTG 2 cut(s) 650, 873
CciI TCATGA 1 cut(s) 787
Cfr13I GGNCC 3 cut(s) 870, 976, 1036
Csp6I GTAC 4 cut(s) 63, 371, 397, 751
CviAII CATG 6 cut(s) 164, 368, 498, 679, 788, 946
CviQI GTAC 4 cut(s) 63, 371, 397, 751
DdeI CTNAG 6 cut(s) 195, 302, 635, 743, 840, 1113
DpnI GATC 2 cut(s) 123, 409
DpnII GATC 2 cut(s) 121, 407
DraI TTTAAA 3 cut(s) 711, 999, 1015
Eam1104I CTCTTC 2 cut(s) 1044, 1049
EarI CTCTTC 2 cut(s) 1044, 1049
Eco130I CCWWGG 3 cut(s) 99, 125, 1181
Eco47I GGWCC 2 cut(s) 976, 1036
Eco57I CTGAAG 1 cut(s) 1101
EcoNI CCTNNNNNAGG 1 cut(s) 344
EcoO109I RGGNCCY 1 cut(s) 870
EcoT14I CCWWGG 3 cut(s) 99, 125, 1181
ErhI CCWWGG 3 cut(s) 99, 125, 1181
FaeI CATG 6 cut(s) 167, 371, 501, 682, 791, 949
FalI AAGNNNNNCTT 2 cut(s) 1046, 1078
FaqI GGGAC 1 cut(s) 312
FatI CATG 6 cut(s) 163, 367, 497, 678, 787, 945
Fnu4HI GCNGC 2 cut(s) 155, 766
FokI GGATG 2 cut(s) 324, 641
Fsp4HI GCNGC 2 cut(s) 155, 766
FspBI CTAG 2 cut(s) 119, 126
GluI GCNGC 2 cut(s) 155, 766
GsuI CTGGAG 3 cut(s) 365, 504, 849
HaeIII GGCC 2 cut(s) 444, 872
HapII CCGG 2 cut(s) 171, 970
Hin1II CATG 6 cut(s) 167, 371, 501, 682, 791, 949
HincII GTYRAC 1 cut(s) 850
HindII GTYRAC 1 cut(s) 850
HindIII AAGCTT 3 cut(s) 386, 1073, 1145
HinfI GANTC 3 cut(s) 177, 527, 1123
HpaII CCGG 2 cut(s) 171, 970
Hpy166II GTNNAC 3 cut(s) 397, 673, 850
Hpy188III TCNNGA 7 cut(s) 89, 536, 591, 611, 626, 788, 1163
Hpy8I GTNNAC 3 cut(s) 397, 673, 850
HpyAV CCTTC 6 cut(s) 168, 286, 390, 497, 790, 794
HpyCH4III ACNGT 5 cut(s) 46, 78, 434, 652, 847
HpyCH4IV ACGT 1 cut(s) 693
HpyCH4V TGCA 9 cut(s) 4, 465, 497, 548, 678, 768, 816, 857, 876
HpyF10VI GCNNNNNNNGC 1 cut(s) 869
HpyF3I CTNAG 6 cut(s) 195, 302, 635, 743, 840, 1113
HpySE526I ACGT 1 cut(s) 693
Hsp92II CATG 6 cut(s) 167, 371, 501, 682, 791, 949
Kzo9I GATC 2 cut(s) 121, 407
Lsp1109I GCAGC 2 cut(s) 141, 752
LweI GCATC 2 cut(s) 448, 850
MaeI CTAG 2 cut(s) 119, 126
MaeII ACGT 1 cut(s) 693
MaeIII GTNAC 3 cut(s) 166, 655, 896
MalI GATC 2 cut(s) 123, 409
MbiI CCGCTC 1 cut(s) 1043
MboI GATC 2 cut(s) 121, 407
MboII GAAGA 9 cut(s) 72, 182, 355, 521, 677, 1058, 1061, 1066, 1132
MflI RGATCY 1 cut(s) 121
MhlI GDGCHC 1 cut(s) 439
MluCI AATT 4 cut(s) 214, 520, 834, 1095
MlyI GAGTC 1 cut(s) 1117
MroXI GAANNNNTTC 2 cut(s) 531, 779
MseI TTAA 4 cut(s) 390, 710, 998, 1014
MspCI CTTAAG 1 cut(s) 389
MspI CCGG 2 cut(s) 171, 970
MspR9I CCNGG 1 cut(s) 970
MssI GTTTAAAC 1 cut(s) 711
MwoI GCNNNNNNNGC 1 cut(s) 869
NciI CCSGG 1 cut(s) 970
NdeII GATC 2 cut(s) 121, 407
NlaIII CATG 6 cut(s) 167, 371, 501, 682, 791, 949
NlaIV GGNNCC 1 cut(s) 959
NmuCI GTSAC 1 cut(s) 655
NspI RCATGY 2 cut(s) 167, 501
NspV TTCGAA 1 cut(s) 731
PagI TCATGA 1 cut(s) 787
PciI ACATGT 1 cut(s) 163
PdmI GAANNNNTTC 2 cut(s) 531, 779
PfeI GAWTC 2 cut(s) 177, 527
PkrI GCNGC 2 cut(s) 156, 767
PleI GAGTC 1 cut(s) 1117
PmeI GTTTAAAC 1 cut(s) 711
PpsI GAGTC 1 cut(s) 1117
PscI ACATGT 1 cut(s) 163
Psp1406I AACGTT 1 cut(s) 693
PspN4I GGNNCC 1 cut(s) 959
PspPI GGNCC 3 cut(s) 870, 976, 1036
PstI CTGCAG 1 cut(s) 770
PstNI CAGNNNCTG 2 cut(s) 650, 873
PsuI RGATCY 1 cut(s) 121
RsaI GTAC 4 cut(s) 64, 372, 398, 752
RsaNI GTAC 4 cut(s) 63, 371, 397, 751
SaqAI TTAA 4 cut(s) 390, 710, 998, 1014
SatI GCNGC 2 cut(s) 155, 766
Sau3AI GATC 2 cut(s) 121, 407
Sau96I GGNCC 3 cut(s) 870, 976, 1036
ScaI AGTACT 1 cut(s) 752
SchI GAGTC 1 cut(s) 1117
ScrFI CCNGG 1 cut(s) 970
SduI GDGCHC 1 cut(s) 439
SfaNI GCATC 2 cut(s) 448, 850
SfcI CTRYAG 2 cut(s) 42, 766
SfuI TTCGAA 1 cut(s) 731
SinI GGWCC 2 cut(s) 976, 1036
SmlI CTYRAG 2 cut(s) 389, 1148
SmoI CTYRAG 2 cut(s) 389, 1148
Sse9I AATT 4 cut(s) 214, 520, 834, 1095
SsiI CCGC 1 cut(s) 1043
SspMI CTAG 2 cut(s) 119, 126
StyD4I CCNGG 1 cut(s) 968
StyI CCWWGG 3 cut(s) 99, 125, 1181
TaaI ACNGT 5 cut(s) 46, 78, 434, 652, 847
TaiI ACGT 1 cut(s) 696
TaqI TCGA 5 cut(s) 326, 612, 731, 801, 890
TaqII GACCGA 1 cut(s) 1053
TasI AATT 4 cut(s) 214, 520, 834, 1095
TatI WGTACW 3 cut(s) 370, 396, 750
TfiI GAWTC 2 cut(s) 177, 527
Tru1I TTAA 4 cut(s) 390, 710, 998, 1014
Tru9I TTAA 4 cut(s) 390, 710, 998, 1014
TscAI CASTG 2 cut(s) 19, 39
TseFI GTSAC 1 cut(s) 655
TseI GCWGC 2 cut(s) 154, 765
Tsp45I GTSAC 1 cut(s) 655
TspDTI ATGAA 4 cut(s) 776, 804, 962, 1133
TspRI CASTG 2 cut(s) 19, 39
Vha464I CTTAAG 1 cut(s) 389
VpaK11BI GGWCC 2 cut(s) 976, 1036
XagI CCTNNNNNAGG 1 cut(s) 344
XapI RAATTY 2 cut(s) 214, 520
XceI RCATGY 2 cut(s) 167, 501
XmaJI CCTAGG 1 cut(s) 125
XmnI GAANNNNTTC 2 cut(s) 531, 779
XspI CTAG 2 cut(s) 119, 126
ZrmI AGTACT 1 cut(s) 752
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.