FvH4_2g35301

OTU domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000651
Physical Location & Seq
Reverse (-)
26060035 .. 26064369
4335 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g35301.t18

Sequence Viewer

Length: 1032 bp
ATGAAGGTAAATCTTGATTATCAAGGCTATGTGAATACGGTTGTTGGTACTCACATCAACCAGGGTGCAAATAATGTTGTTTGCATGAGCGGCTGTATCGAAATGCAGATGGGCAGTAAGATCTGTAGTGTAGTGTCTCGAGGAGCATCCAGTTCATATTGTTATCGGCTGCAGCCAGGGAAATCGGGAAATAAATTTGGCACTTTGTCCCTCACCAAGAGTCGTCCATCAGAAACTGGCCAAACACCGCATGGAAGCTGTTTCAGGTCTTGCTTCTCCATGGATAGGGGCAACTCACGGTCGTTGACAGTTAATGCTAAAAGAACTCAGAAATGTCTAGAAATTTCATTGGCATGTCGGGGCATGAAAACTAGAATTTTGGTCCCAAGACAAGGAATGCTTCCCAAAATCAAGTGTAATGTGGGCCCAATGTCATGGACACAATGTGGTTATGCTGGTTTAATGTTTGGGTTGCTGATTTGTAATTCTTCTGAGCCAGCACACGCTGAAACAACTCATAAGAATGATGATAAGGAAGATGACGGTGATTTGTCATATTCACATGGAAAAAAAGTTCATACTGACTATTCCATTATTGGTATACCTGGAGATGGAAGGTGCTTGTTCCGCTCTGTTGCTCATGGCGCTTGTTTAAGAGCTGGAAAATCAGCTCCTAGTCAAAGCCTTCAGAGAGAATTAGCAGATGACTTGAGAGCTAGAGTTGCTGATGAGTTTATCAAAAGGAGAGAAGAGACAGAATGGTTTGTTGAAGGTGATTTTGACACATATGTCTCCCAAATAAGAAAGCCACATGTATGGGGAGGGGAGCCCGAATTGTTAATGGCTTCACATGTTCTTCAAATGCCGATCACAGTATACATGCATGATGAAAAGGCTGGCGGCTTAATAACTATTGCCGAGTACGGGCAAGAATATGGGAAGGAAAATCCAATCAGAGTTCTCTATCATGGTTTTGGCCATTATGATGCATTGCATATTCCAGGAGTGAGGAGTGGTAAATCTAGGCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
OTU

Protein Analysis

344

Amino Acids

37.87

Weight (kDa)

8.8

Isoelectric Point (pI)

49.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OTU PF02338 202 - 328 2.7e-29 OTU-like cysteine protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 788
AccBSI CCGCTC 2 cut(s) 90, 630
AccI GTMKAC 2 cut(s) 601, 876
AciI CCGC 4 cut(s) 90, 248, 628, 900
AcoI YGGCCR 2 cut(s) 238, 976
AcsI RAATTY 3 cut(s) 194, 342, 375
AcuI CTGAAG 1 cut(s) 671
AdeI CACNNNGTG 1 cut(s) 446
AfaI GTAC 2 cut(s) 49, 923
AfiI CCNNNNNNNGG 4 cut(s) 285, 392, 611, 924
AflIII ACRYGT 2 cut(s) 811, 850
AgsI TTSAA 2 cut(s) 770, 860
AjnI CCWGG 4 cut(s) 60, 175, 604, 1000
AloI GAACNNNNNNTCC 2 cut(s) 558, 590
AluBI AGCT 4 cut(s) 258, 659, 671, 716
AluI AGCT 4 cut(s) 258, 659, 671, 716
Alw26I GTCTC 3 cut(s) 141, 746, 796
AlwNI CAGNNNCTG 1 cut(s) 236
Ama87I CYCGRG 1 cut(s) 138
AoxI GGCC 3 cut(s) 238, 424, 976
ApaI GGGCCC 1 cut(s) 428
ApeKI GCWGC 2 cut(s) 169, 172
ApoI RAATTY 3 cut(s) 194, 342, 375
AspLEI GCGC 1 cut(s) 647
AspS9I GGNCC 3 cut(s) 382, 424, 425
AsuHPI GGTGA 3 cut(s) 205, 557, 785
AvaI CYCGRG 1 cut(s) 138
AvaII GGWCC 1 cut(s) 382
BaeGI GKGCMC 1 cut(s) 428
BalI TGGCCA 2 cut(s) 240, 978
BanII GRGCYC 2 cut(s) 428, 831
BbvI GCAGC 2 cut(s) 156, 184
BccI CCATC 3 cut(s) 103, 235, 605
BciT130I CCWGG 4 cut(s) 62, 177, 606, 1002
BcoDI GTCTC 3 cut(s) 141, 746, 796
BfaI CTAG 5 cut(s) 338, 372, 675, 717, 1023
BfmI CTRYAG 2 cut(s) 124, 170
BfoI RGCGCY 1 cut(s) 648
BglII AGATCT 1 cut(s) 120
BisI GCNGC 4 cut(s) 91, 170, 173, 901
BlsI GCNGC 4 cut(s) 92, 171, 174, 902
Bme1390I CCNGG 4 cut(s) 62, 177, 606, 1002
Bme18I GGWCC 1 cut(s) 382
BmeT110I CYCGRG 1 cut(s) 138
BmgT120I GGNCC 3 cut(s) 382, 424, 425
BmiI GGNNCC 3 cut(s) 384, 426, 828
BmrFI CCNGG 4 cut(s) 62, 177, 606, 1002
BmsI GCATC 2 cut(s) 155, 976
BpmI CTGGAG 1 cut(s) 627
BpuEI CTTGAG 1 cut(s) 730
BsaJI CCNNGG 3 cut(s) 61, 176, 279
Bsc4I CCNNNNNNNGG 4 cut(s) 285, 392, 611, 924
Bse1I ACTGG 2 cut(s) 150, 241
Bse3DI GCAATG 1 cut(s) 989
BseBI CCWGG 4 cut(s) 62, 177, 606, 1002
BseDI CCNNGG 3 cut(s) 61, 176, 279
BseGI GGATG 1 cut(s) 146
BseLI CCNNNNNNNGG 4 cut(s) 285, 392, 611, 924
BseMI GCAATG 1 cut(s) 989
BseMII CTCAG 2 cut(s) 341, 483
BseNI ACTGG 2 cut(s) 150, 241
BseRI GAGGAG 2 cut(s) 156, 1024
BseSI GKGCMC 1 cut(s) 428
BseXI GCAGC 2 cut(s) 156, 184
Bsh1285I CGRYCG 1 cut(s) 302
BshFI GGCC 3 cut(s) 240, 426, 978
BsiEI CGRYCG 1 cut(s) 302
BsiHKCI CYCGRG 1 cut(s) 138
BslFI GGGAC 2 cut(s) 193, 368
BslI CCNNNNNNNGG 4 cut(s) 285, 392, 611, 924
BsmAI GTCTC 3 cut(s) 141, 746, 796
BsmFI GGGAC 2 cut(s) 193, 368
BsmI GAATGC 1 cut(s) 402
BsnI GGCC 3 cut(s) 240, 426, 978
BsoBI CYCGRG 1 cut(s) 138
Bsp120I GGGCCC 1 cut(s) 424
Bsp1286I GDGCHC 2 cut(s) 428, 831
Bsp143I GATC 2 cut(s) 120, 867
Bsp19I CCATGG 1 cut(s) 279
BspACI CCGC 4 cut(s) 90, 248, 628, 900
BspANI GGCC 3 cut(s) 240, 426, 978
BspCNI CTCAG 2 cut(s) 340, 484
BspLI GGNNCC 3 cut(s) 384, 426, 828
BspMAI CTGCAG 1 cut(s) 174
BsrBI CCGCTC 2 cut(s) 90, 630
BsrDI GCAATG 1 cut(s) 989
BsrI ACTGG 2 cut(s) 150, 241
BssECI CCNNGG 3 cut(s) 61, 176, 279
BssMI GATC 2 cut(s) 120, 867
BssNAI GTATAC 2 cut(s) 602, 877
BssT1I CCWWGG 1 cut(s) 279
Bst1107I GTATAC 2 cut(s) 602, 877
Bst2UI CCWGG 4 cut(s) 62, 177, 606, 1002
Bst4CI ACNGT 5 cut(s) 40, 300, 310, 545, 874
Bst6I CTCTTC 1 cut(s) 744
BstC8I GCNNGC 2 cut(s) 498, 898
BstDEI CTNAG 2 cut(s) 327, 492
BstDSI CCRYGG 1 cut(s) 279
BstF5I GGATG 1 cut(s) 146
BstH2I RGCGCY 1 cut(s) 648
BstHHI GCGC 1 cut(s) 647
BstKTI GATC 2 cut(s) 123, 870
BstMAI GTCTC 3 cut(s) 141, 746, 796
BstMBI GATC 2 cut(s) 120, 867
BstMCI CGRYCG 1 cut(s) 302
BstMWI GCNNNNNNNGC 4 cut(s) 90, 627, 644, 722
BstNI CCWGG 4 cut(s) 62, 177, 606, 1002
BstNSI RCATGY 4 cut(s) 357, 815, 854, 883
BstSCI CCNGG 4 cut(s) 60, 175, 604, 1000
BstSFI CTRYAG 2 cut(s) 124, 170
BstSLI GKGCMC 1 cut(s) 428
BstV1I GCAGC 2 cut(s) 156, 184
BstX2I RGATCY 1 cut(s) 120
BstXI CCANNNNNNTGG 2 cut(s) 435, 816
BstYI RGATCY 1 cut(s) 120
BstZ17I GTATAC 2 cut(s) 602, 877
BsuRI GGCC 3 cut(s) 240, 426, 978
BtgI CCRYGG 1 cut(s) 279
BtsCI GGATG 1 cut(s) 146
Cac8I GCNNGC 2 cut(s) 498, 898
CaiI CAGNNNCTG 1 cut(s) 236
CfoI GCGC 1 cut(s) 647
Cfr13I GGNCC 3 cut(s) 382, 424, 425
Csp6I GTAC 2 cut(s) 48, 922
CviQI GTAC 2 cut(s) 48, 922
DdeI CTNAG 2 cut(s) 327, 492
DpnI GATC 2 cut(s) 122, 869
DpnII GATC 2 cut(s) 120, 867
DraIII CACNNNGTG 1 cut(s) 446
DrdI GACNNNNNNGTC 1 cut(s) 788
DseDI GACNNNNNNGTC 1 cut(s) 788
EaeI YGGCCR 2 cut(s) 238, 976
Eam1104I CTCTTC 1 cut(s) 744
EarI CTCTTC 1 cut(s) 744
Eco130I CCWWGG 1 cut(s) 279
Eco24I GRGCYC 2 cut(s) 428, 831
Eco47I GGWCC 1 cut(s) 382
Eco57I CTGAAG 1 cut(s) 671
Eco88I CYCGRG 1 cut(s) 138
EcoRII CCWGG 4 cut(s) 60, 175, 604, 1000
EcoT14I CCWWGG 1 cut(s) 279
EcoT22I ATGCAT 2 cut(s) 885, 991
EcoT38I GRGCYC 2 cut(s) 428, 831
ErhI CCWWGG 1 cut(s) 279
FalI AAGNNNNNCTT 2 cut(s) 384, 416
FaqI GGGAC 2 cut(s) 193, 368
FauNDI CATATG 1 cut(s) 787
FblI GTMKAC 2 cut(s) 601, 876
Fnu4HI GCNGC 4 cut(s) 91, 170, 173, 901
FokI GGATG 1 cut(s) 133
FriOI GRGCYC 2 cut(s) 428, 831
Fsp4HI GCNGC 4 cut(s) 91, 170, 173, 901
FspBI CTAG 5 cut(s) 338, 372, 675, 717, 1023
GlaI GCGC 1 cut(s) 646
GluI GCNGC 4 cut(s) 91, 170, 173, 901
GsuI CTGGAG 1 cut(s) 627
HaeII RGCGCY 1 cut(s) 648
HaeIII GGCC 3 cut(s) 240, 426, 978
HhaI GCGC 1 cut(s) 647
Hin6I GCGC 1 cut(s) 645
HinP1I GCGC 1 cut(s) 645
HincII GTYRAC 1 cut(s) 306
HindII GTYRAC 1 cut(s) 306
HinfI GANTC 1 cut(s) 220
HphI GGTGA 3 cut(s) 205, 557, 785
Hpy166II GTNNAC 3 cut(s) 306, 602, 877
Hpy188I TCNGA 5 cut(s) 232, 330, 493, 690, 956
Hpy188III TCNNGA 4 cut(s) 14, 138, 186, 338
Hpy8I GTNNAC 3 cut(s) 306, 602, 877
HpyAV CCTTC 4 cut(s) 609, 695, 764, 934
HpyCH4III ACNGT 5 cut(s) 40, 300, 310, 545, 874
HpyCH4V TGCA 7 cut(s) 68, 84, 106, 172, 883, 989, 994
HpyF10VI GCNNNNNNNGC 4 cut(s) 90, 627, 644, 722
HpyF3I CTNAG 2 cut(s) 327, 492
HspAI GCGC 1 cut(s) 645
Kzo9I GATC 2 cut(s) 120, 867
LmnI GCTCC 3 cut(s) 143, 676, 826
Lsp1109I GCAGC 2 cut(s) 156, 184
LweI GCATC 2 cut(s) 155, 976
MaeI CTAG 5 cut(s) 338, 372, 675, 717, 1023
MalI GATC 2 cut(s) 122, 869
MbiI CCGCTC 2 cut(s) 90, 630
MboI GATC 2 cut(s) 120, 867
MboII GAAGA 4 cut(s) 480, 548, 761, 848
MflI RGATCY 1 cut(s) 120
MhlI GDGCHC 2 cut(s) 428, 831
MlsI TGGCCA 2 cut(s) 240, 978
MluCI AATT 6 cut(s) 194, 342, 375, 484, 695, 833
MluNI TGGCCA 2 cut(s) 240, 978
MlyI GAGTC 1 cut(s) 229
MnlI CCTC 4 cut(s) 134, 221, 815, 1002
Mox20I TGGCCA 2 cut(s) 240, 978
Mph1103I ATGCAT 2 cut(s) 885, 991
MscI TGGCCA 2 cut(s) 240, 978
MseI TTAA 5 cut(s) 312, 461, 653, 839, 905
MslI CAYNNNNRTG 4 cut(s) 352, 522, 814, 984
Msp20I TGGCCA 2 cut(s) 240, 978
MspR9I CCNGG 4 cut(s) 62, 177, 606, 1002
Mva1269I GAATGC 1 cut(s) 402
MvaI CCWGG 4 cut(s) 62, 177, 606, 1002
MwoI GCNNNNNNNGC 4 cut(s) 90, 627, 644, 722
NcoI CCATGG 1 cut(s) 279
NdeI CATATG 1 cut(s) 787
NdeII GATC 2 cut(s) 120, 867
NlaIV GGNNCC 3 cut(s) 384, 426, 828
NmeAIII GCCGAG 1 cut(s) 943
NsiI ATGCAT 2 cut(s) 885, 991
NspI RCATGY 4 cut(s) 357, 815, 854, 883
PaeR7I CTCGAG 1 cut(s) 138
PciI ACATGT 2 cut(s) 811, 850
PctI GAATGC 1 cut(s) 402
PfoI TCCNGGA 1 cut(s) 1000
PkrI GCNGC 4 cut(s) 92, 171, 174, 902
PleI GAGTC 1 cut(s) 228
PpsI GAGTC 1 cut(s) 228
PscI ACATGT 2 cut(s) 811, 850
Psp6I CCWGG 4 cut(s) 60, 175, 604, 1000
PspGI CCWGG 4 cut(s) 60, 175, 604, 1000
PspN4I GGNNCC 3 cut(s) 384, 426, 828
PspOMI GGGCCC 1 cut(s) 424
PspPI GGNCC 3 cut(s) 382, 424, 425
PstI CTGCAG 1 cut(s) 174
PstNI CAGNNNCTG 1 cut(s) 236
PsuI RGATCY 1 cut(s) 120
RsaI GTAC 2 cut(s) 49, 923
RsaNI GTAC 2 cut(s) 48, 922
RseI CAYNNNNRTG 4 cut(s) 352, 522, 814, 984
SaqAI TTAA 5 cut(s) 312, 461, 653, 839, 905
SatI GCNGC 4 cut(s) 91, 170, 173, 901
Sau3AI GATC 2 cut(s) 120, 867
Sau96I GGNCC 3 cut(s) 382, 424, 425
SchI GAGTC 1 cut(s) 229
ScrFI CCNGG 4 cut(s) 62, 177, 606, 1002
SduI GDGCHC 2 cut(s) 428, 831
SetI ASST 9 cut(s) 9, 260, 269, 607, 620, 661, 673, 718, 775
SfaNI GCATC 2 cut(s) 155, 976
SfcI CTRYAG 2 cut(s) 124, 170
Sfr274I CTCGAG 1 cut(s) 138
SinI GGWCC 1 cut(s) 382
SlaI CTCGAG 1 cut(s) 138
SmiMI CAYNNNNRTG 4 cut(s) 352, 522, 814, 984
SmlI CTYRAG 2 cut(s) 138, 709
SmoI CTYRAG 2 cut(s) 138, 709
Sse9I AATT 6 cut(s) 194, 342, 375, 484, 695, 833
SsiI CCGC 4 cut(s) 90, 248, 628, 900
SspMI CTAG 5 cut(s) 338, 372, 675, 717, 1023
StyD4I CCNGG 4 cut(s) 60, 175, 604, 1000
StyI CCWWGG 1 cut(s) 279
TaaI ACNGT 5 cut(s) 40, 300, 310, 545, 874
TaqI TCGA 2 cut(s) 99, 139
TasI AATT 6 cut(s) 194, 342, 375, 484, 695, 833
TauI GCSGC 2 cut(s) 93, 903
Tru1I TTAA 5 cut(s) 312, 461, 653, 839, 905
Tru9I TTAA 5 cut(s) 312, 461, 653, 839, 905
TseI GCWGC 2 cut(s) 169, 172
TspDTI ATGAA 6 cut(s) 17, 144, 336, 380, 566, 903
VpaK11BI GGWCC 1 cut(s) 382
XapI RAATTY 3 cut(s) 194, 342, 375
XbaI TCTAGA 1 cut(s) 337
XceI RCATGY 4 cut(s) 357, 815, 854, 883
XcmI CCANNNNNNNNNTGG 1 cut(s) 248
XhoI CTCGAG 1 cut(s) 138
XmiI GTMKAC 2 cut(s) 601, 876
XspI CTAG 5 cut(s) 338, 372, 675, 717, 1023
Zsp2I ATGCAT 2 cut(s) 885, 991
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.