Rh4DG306600

OTU domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0000651
Physical Location & Seq
Forward (+)
52880476 .. 52883016
2541 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG306600.1

Sequence Viewer

Length: 576 bp
ATGCTGGCTAATTTGGCATCGCACTTGAAGACCAAAAGTCGTGCACTTTGGAGGAATTGTTCTCACATCACTTGTGGGCTTATTCGACGGTTCGCAATCAGTTGCATATCAAAATCAAAATTACCAAGAAGCAAAGAAGGCATACGCGGAGATGGAAGGTGTTTGTTTCGTTCTGTGGTTCATGGAGCTTGTCTAAGAGCAGGGAAGCCATCTCCAAGTGACAGCCATCAGAAAGAACTTGCAGATGAGCTTAGAGAGAAGGTAGCAGATGAATTCATTAAGAGGAGGGCTGACACTGAATGGTTTCTTGAAGATGATTTTGAGAGATACGTTGTACAGATGCGGCAACCCCACATTTGGGGAGGCGAGCCTGAGTTGCTTATGTCCTCGCATGTCCTGCAGATACCGATTACAGTTTATATGCGGGACAAGAATTCTAGGAGTCTCAGAATCATAGCTGAATATGGCAAAGAGTATGGTAAGGATAACCCTATCCGTGTACTTTATCATGGCTATGGACACTACGATGCATTGCGTGGTCCAAGTGCAACTACTGGTGCAGCGTCCAAGCCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
OTU

Protein Analysis

191

Amino Acids

21.72

Weight (kDa)

9.53

Isoelectric Point (pI)

52.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OTU PF02338 49 - 175 2.2e-28 OTU-like cysteine protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 147
AciI CCGC 3 cut(s) 147, 343, 424
AcsI RAATTY 2 cut(s) 272, 433
AfaI GTAC 2 cut(s) 336, 501
AfiI CCNNNNNNNGG 2 cut(s) 357, 358
AgsI TTSAA 2 cut(s) 28, 311
AhdI GACNNNNNGTC 1 cut(s) 36
AloI GAACNNNNNNTCC 2 cut(s) 43, 75
AluBI AGCT 3 cut(s) 188, 250, 458
AluI AGCT 3 cut(s) 188, 250, 458
Alw21I GWGCWC 1 cut(s) 46
Alw26I GTCTC 1 cut(s) 449
Alw44I GTGCAC 1 cut(s) 42
ApaLI GTGCAC 1 cut(s) 42
ApeKI GCWGC 1 cut(s) 560
ApoI RAATTY 2 cut(s) 272, 433
Asp700I GAANNNNTTC 1 cut(s) 303
AspS9I GGNCC 1 cut(s) 539
AvaII GGWCC 1 cut(s) 539
BaeGI GKGCMC 1 cut(s) 46
BbsI GAAGAC 1 cut(s) 35
Bbv12I GWGCWC 1 cut(s) 46
BbvI GCAGC 1 cut(s) 572
BccI CCATC 3 cut(s) 146, 217, 234
BceAI ACGGC 1 cut(s) 556
BcoDI GTCTC 1 cut(s) 449
BfaI CTAG 1 cut(s) 438
BfmI CTRYAG 1 cut(s) 398
BisI GCNGC 2 cut(s) 344, 561
BlsI GCNGC 2 cut(s) 345, 562
Bme18I GGWCC 1 cut(s) 539
BmeRI GACNNNNNGTC 1 cut(s) 36
BmgT120I GGNCC 1 cut(s) 539
BmsI GCATC 3 cut(s) 26, 330, 517
BpiI GAAGAC 1 cut(s) 35
BsaXI ACNNNNNCTCC 2 cut(s) 43, 73
Bsc4I CCNNNNNNNGG 2 cut(s) 357, 358
Bse1I ACTGG 1 cut(s) 559
Bse3DI GCAATG 1 cut(s) 530
BseLI CCNNNNNNNGG 2 cut(s) 357, 358
BseMI GCAATG 1 cut(s) 530
BseMII CTCAG 2 cut(s) 363, 460
BseNI ACTGG 1 cut(s) 559
BseRI GAGGAG 1 cut(s) 298
BseSI GKGCMC 1 cut(s) 46
BseXI GCAGC 1 cut(s) 572
Bsh1236I CGCG 1 cut(s) 147
BsiHKAI GWGCWC 1 cut(s) 46
BslFI GGGAC 1 cut(s) 440
BslI CCNNNNNNNGG 2 cut(s) 357, 358
BsmAI GTCTC 1 cut(s) 449
BsmFI GGGAC 1 cut(s) 440
Bsp1286I GDGCHC 1 cut(s) 46
Bsp1407I TGTACA 1 cut(s) 334
BspACI CCGC 3 cut(s) 147, 343, 424
BspCNI CTCAG 2 cut(s) 364, 459
BspFNI CGCG 1 cut(s) 147
BspMAI CTGCAG 1 cut(s) 402
BsrDI GCAATG 1 cut(s) 530
BsrGI TGTACA 1 cut(s) 334
BsrI ACTGG 1 cut(s) 559
Bst4CI ACNGT 2 cut(s) 90, 415
BstAPI GCANNNNNTGC 1 cut(s) 397
BstAUI TGTACA 1 cut(s) 334
BstC8I GCNNGC 2 cut(s) 6, 368
BstDEI CTNAG 4 cut(s) 194, 251, 372, 446
BstFNI CGCG 1 cut(s) 147
BstMAI GTCTC 1 cut(s) 449
BstMWI GCNNNNNNNGC 4 cut(s) 14, 138, 376, 397
BstNSI RCATGY 1 cut(s) 395
BstSFI CTRYAG 1 cut(s) 398
BstSLI GKGCMC 1 cut(s) 46
BstUI CGCG 1 cut(s) 147
BstV1I GCAGC 1 cut(s) 572
BstV2I GAAGAC 1 cut(s) 35
BtgZI GCGATG 1 cut(s) 3
BtsIMutI CAGTG 1 cut(s) 294
Cac8I GCNNGC 2 cut(s) 6, 368
Cfr13I GGNCC 1 cut(s) 539
CseI GACGC 1 cut(s) 552
Csp6I GTAC 2 cut(s) 335, 500
CviAII CATG 3 cut(s) 182, 392, 509
CviQI GTAC 2 cut(s) 335, 500
DdeI CTNAG 4 cut(s) 194, 251, 372, 446
DriI GACNNNNNGTC 1 cut(s) 36
Eam1105I GACNNNNNGTC 1 cut(s) 36
Eco47I GGWCC 1 cut(s) 539
EcoRI GAATTC 2 cut(s) 272, 433
EcoT22I ATGCAT 1 cut(s) 532
FaeI CATG 3 cut(s) 185, 395, 512
FaqI GGGAC 1 cut(s) 440
FatI CATG 3 cut(s) 181, 391, 508
FauI CCCGC 1 cut(s) 417
Fnu4HI GCNGC 2 cut(s) 344, 561
Fsp4HI GCNGC 2 cut(s) 344, 561
FspBI CTAG 1 cut(s) 438
GluI GCNGC 2 cut(s) 344, 561
HgaI GACGC 1 cut(s) 552
Hin1II CATG 3 cut(s) 185, 395, 512
HinfI GANTC 2 cut(s) 442, 450
Hpy166II GTNNAC 2 cut(s) 44, 500
Hpy188I TCNGA 2 cut(s) 231, 449
Hpy188III TCNNGA 1 cut(s) 308
Hpy8I GTNNAC 2 cut(s) 44, 500
Hpy99I CGWCG 1 cut(s) 90
HpyAV CCTTC 3 cut(s) 131, 150, 253
HpyCH4III ACNGT 2 cut(s) 90, 415
HpyCH4IV ACGT 1 cut(s) 330
HpyCH4V TGCA 7 cut(s) 44, 105, 242, 400, 530, 548, 560
HpyF10VI GCNNNNNNNGC 4 cut(s) 14, 138, 376, 397
HpyF3I CTNAG 4 cut(s) 194, 251, 372, 446
HpySE526I ACGT 1 cut(s) 330
Hsp92II CATG 3 cut(s) 185, 395, 512
LmnI GCTCC 1 cut(s) 185
LpnPI CCDG 4 cut(s) 186, 384, 410, 540
Lsp1109I GCAGC 1 cut(s) 572
LweI GCATC 3 cut(s) 26, 330, 517
MaeI CTAG 1 cut(s) 438
MaeII ACGT 1 cut(s) 330
MaeIII GTNAC 1 cut(s) 218
MboII GAAGA 2 cut(s) 40, 323
MhlI GDGCHC 1 cut(s) 46
MluCI AATT 5 cut(s) 10, 55, 119, 272, 433
MlyI GAGTC 1 cut(s) 451
MnlI CCTC 5 cut(s) 45, 276, 279, 356, 397
Mph1103I ATGCAT 1 cut(s) 532
MroXI GAANNNNTTC 1 cut(s) 303
MseI TTAA 1 cut(s) 279
MslI CAYNNNNRTG 2 cut(s) 513, 525
MvnI CGCG 1 cut(s) 147
MwoI GCNNNNNNNGC 4 cut(s) 14, 138, 376, 397
NlaIII CATG 3 cut(s) 185, 395, 512
NmuCI GTSAC 1 cut(s) 218
NsiI ATGCAT 1 cut(s) 532
NspI RCATGY 1 cut(s) 395
PdmI GAANNNNTTC 1 cut(s) 303
PfeI GAWTC 1 cut(s) 450
PkrI GCNGC 2 cut(s) 345, 562
PleI GAGTC 1 cut(s) 450
PpsI GAGTC 1 cut(s) 450
PspPI GGNCC 1 cut(s) 539
PstI CTGCAG 1 cut(s) 402
RsaI GTAC 2 cut(s) 336, 501
RsaNI GTAC 2 cut(s) 335, 500
RseI CAYNNNNRTG 2 cut(s) 513, 525
SaqAI TTAA 1 cut(s) 279
SatI GCNGC 2 cut(s) 344, 561
Sau96I GGNCC 1 cut(s) 539
SchI GAGTC 1 cut(s) 451
SduI GDGCHC 1 cut(s) 46
SetI ASST 6 cut(s) 161, 190, 252, 264, 333, 460
SfaNI GCATC 3 cut(s) 26, 330, 517
SfcI CTRYAG 1 cut(s) 398
SinI GGWCC 1 cut(s) 539
SmiMI CAYNNNNRTG 2 cut(s) 513, 525
Sse9I AATT 5 cut(s) 10, 55, 119, 272, 433
SsiI CCGC 3 cut(s) 147, 343, 424
SspMI CTAG 1 cut(s) 438
TaaI ACNGT 2 cut(s) 90, 415
TaiI ACGT 1 cut(s) 333
TaqI TCGA 1 cut(s) 85
TasI AATT 5 cut(s) 10, 55, 119, 272, 433
TatI WGTACW 2 cut(s) 334, 499
TauI GCSGC 1 cut(s) 346
TfiI GAWTC 1 cut(s) 450
Tru1I TTAA 1 cut(s) 279
Tru9I TTAA 1 cut(s) 279
TscAI CASTG 1 cut(s) 301
TseFI GTSAC 1 cut(s) 218
TseI GCWGC 1 cut(s) 560
Tsp45I GTSAC 1 cut(s) 218
TspDTI ATGAA 3 cut(s) 170, 265, 285
TspGWI ACGGA 1 cut(s) 485
TspRI CASTG 1 cut(s) 301
VneI GTGCAC 1 cut(s) 42
VpaK11BI GGWCC 1 cut(s) 539
XapI RAATTY 2 cut(s) 272, 433
XceI RCATGY 1 cut(s) 395
XmnI GAANNNNTTC 1 cut(s) 303
XspI CTAG 1 cut(s) 438
Zsp2I ATGCAT 1 cut(s) 532
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.