Rh3CG266400

OTU domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0000651
Physical Location & Seq
Reverse (-)
26178149 .. 26184866
6718 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3CG266400.1

Sequence Viewer

Length: 1629 bp
ATGAAGGTAAATCTTGATTATCAAGGCTATGTGAATACGATTGTTTGTACTCACATCGACCAGGGTGCAAAGAATGTTGTCTGCATGAGTGGCAGTATTGAAGTGCAGATGGGCAGTAAGATCTGTAGTGTAGTGTCTCGAGGAGCATCCAGTTCATGTTGTTACCGACTGCAGCCAGGGAAATCGGGAAATAAATTTGGTGGCTTATCCCTCACCAAGAGTCGTCCATCTGGTCAAACACCCCATGGAAGCTGTTTCAGGTCTTACTTCTCCACGGATATGGGCAACTCACGGTCATTAACAGTTAATGCTAGAAAACCTCAGAAACGATGTCTAGAAATTTCATTGGCATGTCAGGGTATGAAAACGAGATTTTTGGTCCCAAGACAAGGAATGCTTCCCAAAATCAAGTGTAATGTGGGCCCACTGTCGTGGCCACAAGGATGTGCGTCTGCTGGTTTGATTTTTGGGTTGCTGATTTGTAATTCTTCTGAGCCAACACACGCTGAAGCAGCTTGTAAAACTGATGATAAGGAATATGACGATGATTTGTCATATTCACATGGAAAAAAAGTTTATACTGATTATTCCATTATTGGAATCCCTGGAGATGGAAGGTGCATGTTCCGCTCTGTTGCTCATGGCGCTTGTTTGCGAGCTGGAAAATCAGCTCCTAGTCCAAGCCTTCAGAGAGAATTAGCAGATGACTTGAGAGCTAGAGTTGCAGATGAGTTTATCAAAAGGAGAGAAGAGACAGAATGGTTTGTTGAAGGTGATTTTGACACATATGTTTCCCAAATAAGAAAGCCACATGTATGGGGAGGCGAGCCCGAATTGTTAATGGCTTCACATGTTCTTGAAATGCCGATCACAGTATACATGTATGATGAAAAGGCTGGCGGCTTGATAACCATTGCCGAGTATGGGCAAGAGTATGGGAAGGAAAATCCAATCAGAGTTCTCTATCATGGTTTTGGCCATTATGATGCATTGCATATTCCGGGAAAGAGTGGTGGGTGTTGGAAGTTCATGCACATAGCGAATCTTGCAGAGCTGCTCGGTTCGTCAACGGTGGCACTGGTCCAAGATAAATCTGAGTTTTCCGAAGACGAGCTATTGTCTGTTGTTATCATGAAGAATGGTCAGAAAGTTATTTGCAGCTCACAAACTGGGAATATATTGTTGTATTCATGGGGTTGTTTCAAGGATTGCAGTGATCGTTTTATTGATCTCTCTCCAAATTCTGTTGATGCTTTGTTGAAGCTTGATGAAGATAGACTTATCGCTGGATCTGAGACCGGACTTATCAGCCTAGTAGGGATATTACCAAACAGAATCATCCAACCAATTGCAGAGCACTCAGAGTATCCGGTAGAGCGTCTTGCTTTTTCTCATGATAAGAAGTTCCTGGGCAGTATAGCACATGATCAGATGTTGAAGCTGTGGGATATGGATGATATATTGCAAGGCTCTGGAAATACTTCAAGGACTCAAGCAACTGTGGTGGACAGTGATAGTGATGAGATGGATACAGATATTAATACTCCAAAGTCTAGAAAAGGAACAAAGAGAAAAAATGCAAGCAAAGATCATGTTATGGGTTCACACAACTTTTTTGCCGATTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
OTU

Protein Analysis

542

Amino Acids

59.71

Weight (kDa)

6.47

Isoelectric Point (pI)

42.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OTU PF02338 202 - 328 1.8e-28 OTU-like cysteine protease
WDR55 PF24796 361 - 485 4.8e-37 WDR55
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 630
AccI GTMKAC 1 cut(s) 876
AciI CCGC 2 cut(s) 628, 900
AclWI GGATC 1 cut(s) 1297
AcoI YGGCCR 2 cut(s) 434, 976
AcsI RAATTY 3 cut(s) 194, 339, 1240
AcuI CTGAAG 2 cut(s) 528, 671
AfaI GTAC 1 cut(s) 49
AfiI CCNNNNNNNGG 3 cut(s) 389, 611, 924
AflIII ACRYGT 3 cut(s) 811, 850, 879
AgsI TTSAA 7 cut(s) 101, 770, 860, 1204, 1261, 1438, 1485
AjnI CCWGG 4 cut(s) 60, 175, 604, 1407
AleI CACNNNNGTG 1 cut(s) 430
Alw21I GWGCWC 1 cut(s) 1359
Alw26I GTCTC 3 cut(s) 141, 746, 1289
AlwI GGATC 1 cut(s) 1297
Ama87I CYCGRG 1 cut(s) 138
AoxI GGCC 3 cut(s) 421, 434, 976
ApaI GGGCCC 1 cut(s) 425
ApeKI GCWGC 4 cut(s) 172, 512, 1054, 1158
ApoI RAATTY 3 cut(s) 194, 339, 1240
AseI ATTAAT 1 cut(s) 1539
Asp700I GAANNNNTTC 1 cut(s) 1480
AspLEI GCGC 1 cut(s) 647
AspS9I GGNCC 4 cut(s) 379, 421, 422, 1081
AsuC2I CCSGG 1 cut(s) 1002
AsuHPI GGTGA 2 cut(s) 205, 785
AvaI CYCGRG 1 cut(s) 138
AvaII GGWCC 2 cut(s) 379, 1081
BaeGI GKGCMC 1 cut(s) 425
BalI TGGCCA 2 cut(s) 436, 978
BanII GRGCYC 2 cut(s) 425, 831
BbsI GAAGAC 1 cut(s) 1113
Bbv12I GWGCWC 1 cut(s) 1359
BbvI GCAGC 4 cut(s) 184, 524, 1041, 1170
BccI CCATC 4 cut(s) 103, 235, 605, 1519
BcgI CGANNNNNNTGC 2 cut(s) 47, 81
BciT130I CCWGG 4 cut(s) 62, 177, 606, 1409
BciVI GTATCC 2 cut(s) 1377, 1522
BclI TGATCA 1 cut(s) 1426
BcnI CCSGG 1 cut(s) 1002
BcoDI GTCTC 3 cut(s) 141, 746, 1289
BfaI CTAG 6 cut(s) 312, 335, 675, 717, 1313, 1554
BfmI CTRYAG 2 cut(s) 124, 170
BfoI RGCGCY 1 cut(s) 648
BfuI GTATCC 2 cut(s) 1377, 1522
BglII AGATCT 1 cut(s) 120
BisI GCNGC 5 cut(s) 173, 513, 901, 1055, 1159
BlsI GCNGC 5 cut(s) 174, 514, 902, 1056, 1160
Bme1390I CCNGG 5 cut(s) 62, 177, 606, 1002, 1409
Bme18I GGWCC 2 cut(s) 379, 1081
BmeT110I CYCGRG 1 cut(s) 138
BmgT120I GGNCC 4 cut(s) 379, 421, 422, 1081
BmiI GGNNCC 2 cut(s) 381, 423
BmrFI CCNGG 5 cut(s) 62, 177, 606, 1002, 1409
BmrI ACTGGG 1 cut(s) 1179
BmsI GCATC 3 cut(s) 155, 976, 1240
BmuI ACTGGG 1 cut(s) 1179
BpiI GAAGAC 1 cut(s) 1113
BpmI CTGGAG 1 cut(s) 627
BpuEI CTTGAG 2 cut(s) 730, 1476
BpuMI CCSGG 1 cut(s) 1002
BsaI GGTCTC 1 cut(s) 1289
BsaJI CCNNGG 6 cut(s) 61, 176, 244, 273, 604, 1408
BsaWI WCCGGW 2 cut(s) 1298, 1369
Bsc4I CCNNNNNNNGG 3 cut(s) 389, 611, 924
Bse1I ACTGG 3 cut(s) 150, 1083, 1174
Bse3DI GCAATG 2 cut(s) 912, 989
BseBI CCWGG 4 cut(s) 62, 177, 606, 1409
BseDI CCNNGG 6 cut(s) 61, 176, 244, 273, 604, 1408
BseGI GGATG 4 cut(s) 146, 449, 1338, 1459
BseLI CCNNNNNNNGG 3 cut(s) 389, 611, 924
BseMI GCAATG 2 cut(s) 912, 989
BseMII CTCAG 5 cut(s) 335, 483, 1086, 1284, 1374
BseNI ACTGG 3 cut(s) 150, 1083, 1174
BseRI GAGGAG 1 cut(s) 156
BseSI GKGCMC 1 cut(s) 425
BseXI GCAGC 4 cut(s) 184, 524, 1041, 1170
BsgI GTGCAG 1 cut(s) 125
BshFI GGCC 3 cut(s) 423, 436, 978
BsiHKAI GWGCWC 1 cut(s) 1359
BsiHKCI CYCGRG 1 cut(s) 138
BsiSI CCGG 3 cut(s) 1001, 1299, 1370
BslFI GGGAC 1 cut(s) 365
BslI CCNNNNNNNGG 3 cut(s) 389, 611, 924
BsmAI GTCTC 3 cut(s) 141, 746, 1289
BsmFI GGGAC 1 cut(s) 365
BsmI GAATGC 1 cut(s) 399
BsnI GGCC 3 cut(s) 423, 436, 978
Bso31I GGTCTC 1 cut(s) 1289
BsoBI CYCGRG 1 cut(s) 138
Bsp120I GGGCCC 1 cut(s) 421
Bsp1286I GDGCHC 3 cut(s) 425, 831, 1359
Bsp143I GATC 7 cut(s) 120, 867, 1216, 1228, 1289, 1426, 1588
Bsp19I CCATGG 1 cut(s) 244
BspACI CCGC 2 cut(s) 628, 900
BspANI GGCC 3 cut(s) 423, 436, 978
BspCNI CTCAG 5 cut(s) 334, 484, 1087, 1285, 1373
BspHI TCATGA 2 cut(s) 1131, 1393
BspLI GGNNCC 2 cut(s) 381, 423
BspMAI CTGCAG 1 cut(s) 174
BspPI GGATC 1 cut(s) 1297
BspTNI GGTCTC 1 cut(s) 1289
BsrBI CCGCTC 1 cut(s) 630
BsrDI GCAATG 2 cut(s) 912, 989
BsrI ACTGG 3 cut(s) 150, 1083, 1174
BssECI CCNNGG 6 cut(s) 61, 176, 244, 273, 604, 1408
BssMI GATC 7 cut(s) 120, 867, 1216, 1228, 1289, 1426, 1588
BssNAI GTATAC 1 cut(s) 877
BssT1I CCWWGG 1 cut(s) 244
Bst1107I GTATAC 1 cut(s) 877
Bst2UI CCWGG 4 cut(s) 62, 177, 606, 1409
Bst4CI ACNGT 7 cut(s) 294, 304, 429, 874, 1072, 1501, 1511
Bst6I CTCTTC 1 cut(s) 744
BstC8I GCNNGC 4 cut(s) 657, 827, 898, 1582
BstDEI CTNAG 5 cut(s) 321, 492, 1095, 1293, 1360
BstDSI CCRYGG 2 cut(s) 244, 273
BstF5I GGATG 4 cut(s) 146, 449, 1338, 1459
BstH2I RGCGCY 1 cut(s) 648
BstHHI GCGC 1 cut(s) 647
BstKTI GATC 7 cut(s) 123, 870, 1219, 1231, 1292, 1429, 1591
BstMAI GTCTC 3 cut(s) 141, 746, 1289
BstMBI GATC 7 cut(s) 120, 867, 1216, 1228, 1289, 1426, 1588
BstMWI GCNNNNNNNGC 6 cut(s) 90, 512, 627, 644, 722, 1046
BstNI CCWGG 4 cut(s) 62, 177, 606, 1409
BstNSI RCATGY 5 cut(s) 354, 625, 815, 854, 883
BstSCI CCNGG 5 cut(s) 60, 175, 604, 1000, 1407
BstSFI CTRYAG 2 cut(s) 124, 170
BstSLI GKGCMC 1 cut(s) 425
BstV1I GCAGC 4 cut(s) 184, 524, 1041, 1170
BstV2I GAAGAC 1 cut(s) 1113
BstX2I RGATCY 2 cut(s) 120, 1289
BstXI CCANNNNNNTGG 3 cut(s) 280, 432, 816
BstYI RGATCY 2 cut(s) 120, 1289
BstZ17I GTATAC 1 cut(s) 877
BsuI GTATCC 2 cut(s) 1377, 1522
BsuRI GGCC 3 cut(s) 423, 436, 978
BtgI CCRYGG 2 cut(s) 244, 273
BtsCI GGATG 4 cut(s) 146, 449, 1338, 1459
BtsI GCAGTG 1 cut(s) 1219
BtsIMutI CAGTG 4 cut(s) 425, 1076, 1219, 1516
Cac8I GCNNGC 4 cut(s) 657, 827, 898, 1582
CciI TCATGA 2 cut(s) 1131, 1393
CfoI GCGC 1 cut(s) 647
Cfr13I GGNCC 4 cut(s) 379, 421, 422, 1081
CseI GACGC 2 cut(s) 438, 1367
Csp6I GTAC 1 cut(s) 48
CspCI CAANNNNNGTGG 2 cut(s) 1485, 1520
CviQI GTAC 1 cut(s) 48
DdeI CTNAG 5 cut(s) 321, 492, 1095, 1293, 1360
DpnI GATC 7 cut(s) 122, 869, 1218, 1230, 1291, 1428, 1590
DpnII GATC 7 cut(s) 120, 867, 1216, 1228, 1289, 1426, 1588
EaeI YGGCCR 2 cut(s) 434, 976
Eam1104I CTCTTC 1 cut(s) 744
EarI CTCTTC 1 cut(s) 744
Eco130I CCWWGG 1 cut(s) 244
Eco24I GRGCYC 2 cut(s) 425, 831
Eco31I GGTCTC 1 cut(s) 1289
Eco47I GGWCC 2 cut(s) 379, 1081
Eco57I CTGAAG 2 cut(s) 528, 671
Eco88I CYCGRG 1 cut(s) 138
EcoRII CCWGG 4 cut(s) 60, 175, 604, 1407
EcoT14I CCWWGG 1 cut(s) 244
EcoT22I ATGCAT 1 cut(s) 991
EcoT38I GRGCYC 2 cut(s) 425, 831
ErhI CCWWGG 1 cut(s) 244
FalI AAGNNNNNCTT 4 cut(s) 381, 413, 1263, 1295
FaqI GGGAC 1 cut(s) 365
FauNDI CATATG 1 cut(s) 787
FbaI TGATCA 1 cut(s) 1426
FblI GTMKAC 1 cut(s) 876
Fnu4HI GCNGC 5 cut(s) 173, 513, 901, 1055, 1159
FokI GGATG 4 cut(s) 133, 456, 1325, 1466
FriOI GRGCYC 2 cut(s) 425, 831
Fsp4HI GCNGC 5 cut(s) 173, 513, 901, 1055, 1159
FspBI CTAG 6 cut(s) 312, 335, 675, 717, 1313, 1554
GlaI GCGC 1 cut(s) 646
GluI GCNGC 5 cut(s) 173, 513, 901, 1055, 1159
GsuI CTGGAG 1 cut(s) 627
HaeII RGCGCY 1 cut(s) 648
HaeIII GGCC 3 cut(s) 423, 436, 978
HapII CCGG 3 cut(s) 1001, 1299, 1370
HgaI GACGC 2 cut(s) 438, 1367
HhaI GCGC 1 cut(s) 647
Hin6I GCGC 1 cut(s) 645
HinP1I GCGC 1 cut(s) 645
HincII GTYRAC 1 cut(s) 1068
HindII GTYRAC 1 cut(s) 1068
HindIII AAGCTT 1 cut(s) 1262
HinfI GANTC 5 cut(s) 220, 600, 1042, 1335, 1489
HpaII CCGG 3 cut(s) 1001, 1299, 1370
HphI GGTGA 2 cut(s) 205, 785
Hpy166II GTNNAC 4 cut(s) 877, 1068, 1507, 1604
Hpy188III TCNNGA 9 cut(s) 14, 138, 186, 335, 857, 1132, 1394, 1473, 1554
Hpy8I GTNNAC 4 cut(s) 877, 1068, 1507, 1604
HpyAV CCTTC 4 cut(s) 609, 695, 764, 934
HpyCH4III ACNGT 7 cut(s) 294, 304, 429, 874, 1072, 1501, 1511
HpyF10VI GCNNNNNNNGC 6 cut(s) 90, 512, 627, 644, 722, 1046
HpyF3I CTNAG 5 cut(s) 321, 492, 1095, 1293, 1360
HspAI GCGC 1 cut(s) 645
Ksp22I TGATCA 1 cut(s) 1426
Kzo9I GATC 7 cut(s) 120, 867, 1216, 1228, 1289, 1426, 1588
LmnI GCTCC 2 cut(s) 143, 676
Lsp1109I GCAGC 4 cut(s) 184, 524, 1041, 1170
LweI GCATC 3 cut(s) 155, 976, 1240
MaeI CTAG 6 cut(s) 312, 335, 675, 717, 1313, 1554
MaeIII GTNAC 1 cut(s) 161
MalI GATC 7 cut(s) 122, 869, 1218, 1230, 1291, 1428, 1590
MbiI CCGCTC 1 cut(s) 630
MboI GATC 7 cut(s) 120, 867, 1216, 1228, 1289, 1426, 1588
MboII GAAGA 5 cut(s) 480, 761, 1118, 1147, 1283
MfeI CAATTG 1 cut(s) 1347
MflI RGATCY 2 cut(s) 120, 1289
MhlI GDGCHC 3 cut(s) 425, 831, 1359
MlsI TGGCCA 2 cut(s) 436, 978
MluCI AATT 7 cut(s) 194, 339, 484, 695, 833, 1240, 1347
MluNI TGGCCA 2 cut(s) 436, 978
MlyI GAGTC 2 cut(s) 229, 1483
MmeI TCCRAC 2 cut(s) 1001, 1366
MnlI CCTC 4 cut(s) 134, 221, 330, 815
Mox20I TGGCCA 2 cut(s) 436, 978
Mph1103I ATGCAT 1 cut(s) 991
MroXI GAANNNNTTC 1 cut(s) 1480
MscI TGGCCA 2 cut(s) 436, 978
MseI TTAA 4 cut(s) 299, 306, 839, 1539
MslI CAYNNNNRTG 6 cut(s) 278, 349, 430, 442, 814, 984
Msp20I TGGCCA 2 cut(s) 436, 978
MspI CCGG 3 cut(s) 1001, 1299, 1370
MspR9I CCNGG 5 cut(s) 62, 177, 606, 1002, 1409
MunI CAATTG 1 cut(s) 1347
Mva1269I GAATGC 1 cut(s) 399
MvaI CCWGG 4 cut(s) 62, 177, 606, 1409
MwoI GCNNNNNNNGC 6 cut(s) 90, 512, 627, 644, 722, 1046
NciI CCSGG 1 cut(s) 1002
NcoI CCATGG 1 cut(s) 244
NdeI CATATG 1 cut(s) 787
NdeII GATC 7 cut(s) 120, 867, 1216, 1228, 1289, 1426, 1588
NlaIV GGNNCC 2 cut(s) 381, 423
NmeAIII GCCGAG 1 cut(s) 943
NsiI ATGCAT 1 cut(s) 991
NspI RCATGY 5 cut(s) 354, 625, 815, 854, 883
OliI CACNNNNGTG 1 cut(s) 430
PaeR7I CTCGAG 1 cut(s) 138
PagI TCATGA 2 cut(s) 1131, 1393
PciI ACATGT 3 cut(s) 811, 850, 879
PctI GAATGC 1 cut(s) 399
PdmI GAANNNNTTC 1 cut(s) 1480
PfeI GAWTC 3 cut(s) 600, 1042, 1335
PfoI TCCNGGA 1 cut(s) 1000
PkrI GCNGC 5 cut(s) 174, 514, 902, 1056, 1160
PleI GAGTC 2 cut(s) 228, 1483
PpsI GAGTC 2 cut(s) 228, 1483
PscI ACATGT 3 cut(s) 811, 850, 879
PshBI ATTAAT 1 cut(s) 1539
Psp6I CCWGG 4 cut(s) 60, 175, 604, 1407
PspGI CCWGG 4 cut(s) 60, 175, 604, 1407
PspN4I GGNNCC 2 cut(s) 381, 423
PspOMI GGGCCC 1 cut(s) 421
PspPI GGNCC 4 cut(s) 379, 421, 422, 1081
PstI CTGCAG 1 cut(s) 174
PsuI RGATCY 2 cut(s) 120, 1289
RsaI GTAC 1 cut(s) 49
RsaNI GTAC 1 cut(s) 48
RseI CAYNNNNRTG 6 cut(s) 278, 349, 430, 442, 814, 984
SaqAI TTAA 4 cut(s) 299, 306, 839, 1539
SatI GCNGC 5 cut(s) 173, 513, 901, 1055, 1159
Sau3AI GATC 7 cut(s) 120, 867, 1216, 1228, 1289, 1426, 1588
Sau96I GGNCC 4 cut(s) 379, 421, 422, 1081
SchI GAGTC 2 cut(s) 229, 1483
ScrFI CCNGG 5 cut(s) 62, 177, 606, 1002, 1409
SduI GDGCHC 3 cut(s) 425, 831, 1359
SfaNI GCATC 3 cut(s) 155, 976, 1240
SfcI CTRYAG 2 cut(s) 124, 170
Sfr274I CTCGAG 1 cut(s) 138
SinI GGWCC 2 cut(s) 379, 1081
SlaI CTCGAG 1 cut(s) 138
SmiMI CAYNNNNRTG 6 cut(s) 278, 349, 430, 442, 814, 984
SmlI CTYRAG 3 cut(s) 138, 709, 1491
SmoI CTYRAG 3 cut(s) 138, 709, 1491
Sse9I AATT 7 cut(s) 194, 339, 484, 695, 833, 1240, 1347
SsiI CCGC 2 cut(s) 628, 900
SspMI CTAG 6 cut(s) 312, 335, 675, 717, 1313, 1554
StyD4I CCNGG 5 cut(s) 60, 175, 604, 1000, 1407
StyI CCWWGG 1 cut(s) 244
TaaI ACNGT 7 cut(s) 294, 304, 429, 874, 1072, 1501, 1511
TaqI TCGA 2 cut(s) 57, 139
TasI AATT 7 cut(s) 194, 339, 484, 695, 833, 1240, 1347
TatI WGTACW 1 cut(s) 47
TauI GCSGC 1 cut(s) 903
TfiI GAWTC 3 cut(s) 600, 1042, 1335
Tru1I TTAA 4 cut(s) 299, 306, 839, 1539
Tru9I TTAA 4 cut(s) 299, 306, 839, 1539
TscAI CASTG 4 cut(s) 432, 1083, 1219, 1516
TseI GCWGC 4 cut(s) 172, 512, 1054, 1158
TspDTI ATGAA 9 cut(s) 17, 144, 333, 377, 903, 1018, 1148, 1179, 1284
TspGWI ACGGA 1 cut(s) 290
TspRI CASTG 4 cut(s) 432, 1083, 1219, 1516
VpaK11BI GGWCC 2 cut(s) 379, 1081
VspI ATTAAT 1 cut(s) 1539
XapI RAATTY 3 cut(s) 194, 339, 1240
XbaI TCTAGA 2 cut(s) 334, 1553
XceI RCATGY 5 cut(s) 354, 625, 815, 854, 883
XhoI CTCGAG 1 cut(s) 138
XmiI GTMKAC 1 cut(s) 876
XmnI GAANNNNTTC 1 cut(s) 1480
XspI CTAG 6 cut(s) 312, 335, 675, 717, 1313, 1554
Zsp2I ATGCAT 1 cut(s) 991
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.