FvH4_4g24091

OTU domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000651
Physical Location & Seq
Forward (+)
26383974 .. 26386153
2180 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g24091.t5

Sequence Viewer

Length: 609 bp
ATGCTGGCTAATTTGGCATCGCACTTGAAGACCAAAAGCTGTGCACTTTGGAGGAATTGTTCTCGCATCACCTGTCTTATTCGACGGTTCGCAATCAGTTGCATATCACAATCACAGTTAGTAGGAAGTAGAGAAGCAGGCATACGCGGAGATGGAAGGTGTTTGTTTCGATCTGTGGTTCATGGAGCTTGTCTAAGAGCAGGGAAGCCATCCCCAAGTGACAGCTATCAGAAAGAACTTGCAGATGACCTTAGAGCCAAGGTAGCAGATGAATTCATTAAGAGGAGGGCTGACACTGAATGGTTTCTTGAAGATGATTTTGAGTCATACATTGTACAGATGCGAAAGCCTCACATCTGGGGAGGCGAGCCTGAGTTGCTTATGTCCTCCCATGTCCTACAGATACCGATTACAGTTTATATGCGCGACAAGAATTCGAGAAGTCTCAGAATCATAGCTGAGTATGGTCAAGAGTATGGTAATGATAACCCTATCCGTGTACTTTATCATGGCTATGGACACTATGATGCATTGCGCGGTCCAACTCCAACTACCGGTGCAATGTCGAAGCAGCTGTCCAAACAAAGAAGCAGAACAGAGATGCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
OTU

Protein Analysis

203

Amino Acids

23.0

Weight (kDa)

9.3

Isoelectric Point (pI)

60.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OTU PF02338 9 - 135 5.6e-27 OTU-like cysteine protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 147, 426, 537
AciI CCGC 2 cut(s) 147, 537
AcsI RAATTY 2 cut(s) 272, 433
AfaI GTAC 2 cut(s) 336, 501
AfiI CCNNNNNNNGG 1 cut(s) 554
AgeI ACCGGT 1 cut(s) 554
AgsI TTSAA 2 cut(s) 28, 311
AloI GAACNNNNNNTCC 2 cut(s) 43, 75
AluBI AGCT 5 cut(s) 39, 188, 225, 458, 574
AluI AGCT 5 cut(s) 39, 188, 225, 458, 574
Alw21I GWGCWC 1 cut(s) 46
Alw26I GTCTC 1 cut(s) 449
Alw44I GTGCAC 1 cut(s) 42
ApaLI GTGCAC 1 cut(s) 42
ApeKI GCWGC 1 cut(s) 571
ApoI RAATTY 2 cut(s) 272, 433
ArsI GACNNNNNNTTYG 2 cut(s) 560, 592
AsiGI ACCGGT 1 cut(s) 554
Asp700I GAANNNNTTC 1 cut(s) 303
AspLEI GCGC 2 cut(s) 426, 537
AspS9I GGNCC 1 cut(s) 539
AsuHPI GGTGA 1 cut(s) 61
AvaII GGWCC 1 cut(s) 539
BaeGI GKGCMC 1 cut(s) 46
BarI GAAGNNNNNNTAC 2 cut(s) 126, 158
BbsI GAAGAC 1 cut(s) 35
Bbv12I GWGCWC 1 cut(s) 46
BbvI GCAGC 1 cut(s) 583
BccI CCATC 2 cut(s) 146, 217
BcoDI GTCTC 1 cut(s) 449
BfmI CTRYAG 1 cut(s) 398
BisI GCNGC 1 cut(s) 572
BlsI GCNGC 1 cut(s) 573
Bme18I GGWCC 1 cut(s) 539
BmgT120I GGNCC 1 cut(s) 539
BmsI GCATC 5 cut(s) 26, 75, 330, 517, 591
BpiI GAAGAC 1 cut(s) 35
BsaJI CCNNGG 1 cut(s) 258
BsaWI WCCGGW 1 cut(s) 554
BsaXI ACNNNNNCTCC 2 cut(s) 43, 73
Bsc4I CCNNNNNNNGG 1 cut(s) 554
Bse118I RCCGGY 1 cut(s) 554
Bse3DI GCAATG 2 cut(s) 530, 567
BseDI CCNNGG 1 cut(s) 258
BseGI GGATG 1 cut(s) 209
BseLI CCNNNNNNNGG 1 cut(s) 554
BseMI GCAATG 2 cut(s) 530, 567
BseMII CTCAG 3 cut(s) 363, 450, 460
BseRI GAGGAG 1 cut(s) 298
BseSI GKGCMC 1 cut(s) 46
BseXI GCAGC 1 cut(s) 583
Bsh1236I CGCG 3 cut(s) 147, 426, 537
BshTI ACCGGT 1 cut(s) 554
BsiHKAI GWGCWC 1 cut(s) 46
BsiSI CCGG 1 cut(s) 555
BslI CCNNNNNNNGG 1 cut(s) 554
BsmAI GTCTC 1 cut(s) 449
Bsp1286I GDGCHC 1 cut(s) 46
Bsp1407I TGTACA 1 cut(s) 334
Bsp143I GATC 1 cut(s) 170
BspACI CCGC 2 cut(s) 147, 537
BspCNI CTCAG 3 cut(s) 364, 451, 459
BspFNI CGCG 3 cut(s) 147, 426, 537
BsrDI GCAATG 2 cut(s) 530, 567
BsrFI RCCGGY 1 cut(s) 554
BsrGI TGTACA 1 cut(s) 334
BssAI RCCGGY 1 cut(s) 554
BssECI CCNNGG 1 cut(s) 258
BssMI GATC 1 cut(s) 170
BssT1I CCWWGG 1 cut(s) 258
Bst4CI ACNGT 3 cut(s) 87, 117, 415
BstAUI TGTACA 1 cut(s) 334
BstC8I GCNNGC 3 cut(s) 6, 139, 368
BstDEI CTNAG 5 cut(s) 194, 251, 372, 446, 459
BstF5I GGATG 1 cut(s) 209
BstFNI CGCG 3 cut(s) 147, 426, 537
BstHHI GCGC 2 cut(s) 426, 537
BstKTI GATC 1 cut(s) 173
BstMAI GTCTC 1 cut(s) 449
BstMBI GATC 1 cut(s) 170
BstMWI GCNNNNNNNGC 3 cut(s) 14, 263, 376
BstSFI CTRYAG 1 cut(s) 398
BstSLI GKGCMC 1 cut(s) 46
BstUI CGCG 3 cut(s) 147, 426, 537
BstV1I GCAGC 1 cut(s) 583
BstV2I GAAGAC 1 cut(s) 35
BtgZI GCGATG 1 cut(s) 3
BtsCI GGATG 1 cut(s) 209
BtsIMutI CAGTG 1 cut(s) 294
Cac8I GCNNGC 3 cut(s) 6, 139, 368
CfoI GCGC 2 cut(s) 426, 537
Cfr10I RCCGGY 1 cut(s) 554
Cfr13I GGNCC 1 cut(s) 539
Csp6I GTAC 2 cut(s) 335, 500
CspAI ACCGGT 1 cut(s) 554
CviAII CATG 3 cut(s) 182, 392, 509
CviQI GTAC 2 cut(s) 335, 500
DdeI CTNAG 5 cut(s) 194, 251, 372, 446, 459
DpnI GATC 1 cut(s) 172
DpnII GATC 1 cut(s) 170
Eco130I CCWWGG 1 cut(s) 258
Eco47I GGWCC 1 cut(s) 539
EcoRI GAATTC 2 cut(s) 272, 433
EcoT14I CCWWGG 1 cut(s) 258
EcoT22I ATGCAT 1 cut(s) 532
ErhI CCWWGG 1 cut(s) 258
FaeI CATG 3 cut(s) 185, 395, 512
FatI CATG 3 cut(s) 181, 391, 508
Fnu4HI GCNGC 1 cut(s) 572
FokI GGATG 1 cut(s) 196
Fsp4HI GCNGC 1 cut(s) 572
GlaI GCGC 2 cut(s) 425, 536
GluI GCNGC 1 cut(s) 572
HapII CCGG 1 cut(s) 555
HhaI GCGC 2 cut(s) 426, 537
Hin1II CATG 3 cut(s) 185, 395, 512
Hin6I GCGC 2 cut(s) 424, 535
HinP1I GCGC 2 cut(s) 424, 535
HinfI GANTC 2 cut(s) 323, 450
HpaII CCGG 1 cut(s) 555
HphI GGTGA 1 cut(s) 61
Hpy166II GTNNAC 2 cut(s) 44, 500
Hpy188I TCNGA 2 cut(s) 231, 449
Hpy188III TCNNGA 3 cut(s) 308, 438, 470
Hpy8I GTNNAC 2 cut(s) 44, 500
Hpy99I CGWCG 1 cut(s) 87
HpyAV CCTTC 1 cut(s) 150
HpyCH4III ACNGT 3 cut(s) 87, 117, 415
HpyCH4V TGCA 6 cut(s) 44, 102, 242, 530, 560, 604
HpyF10VI GCNNNNNNNGC 3 cut(s) 14, 263, 376
HpyF3I CTNAG 5 cut(s) 194, 251, 372, 446, 459
Hsp92II CATG 3 cut(s) 185, 395, 512
HspAI GCGC 2 cut(s) 424, 535
Kzo9I GATC 1 cut(s) 170
LmnI GCTCC 1 cut(s) 185
LpnPI CCDG 6 cut(s) 85, 123, 186, 343, 384, 568
Lsp1109I GCAGC 1 cut(s) 583
LweI GCATC 5 cut(s) 26, 75, 330, 517, 591
MaeIII GTNAC 1 cut(s) 218
MalI GATC 1 cut(s) 172
MboI GATC 1 cut(s) 170
MboII GAAGA 2 cut(s) 40, 323
MhlI GDGCHC 1 cut(s) 46
MluCI AATT 4 cut(s) 10, 55, 272, 433
MlyI GAGTC 1 cut(s) 332
MmeI TCCRAC 2 cut(s) 566, 572
MnlI CCTC 6 cut(s) 45, 276, 279, 356, 360, 397
Mph1103I ATGCAT 1 cut(s) 532
MroXI GAANNNNTTC 1 cut(s) 303
MseI TTAA 1 cut(s) 279
MslI CAYNNNNRTG 2 cut(s) 513, 525
MspA1I CMGCKG 1 cut(s) 574
MspI CCGG 1 cut(s) 555
MvnI CGCG 3 cut(s) 147, 426, 537
MwoI GCNNNNNNNGC 3 cut(s) 14, 263, 376
NdeII GATC 1 cut(s) 170
NlaIII CATG 3 cut(s) 185, 395, 512
NmuCI GTSAC 1 cut(s) 218
NsiI ATGCAT 1 cut(s) 532
PdmI GAANNNNTTC 1 cut(s) 303
PfeI GAWTC 1 cut(s) 450
PinAI ACCGGT 1 cut(s) 554
PkrI GCNGC 1 cut(s) 573
PleI GAGTC 1 cut(s) 331
PpsI GAGTC 1 cut(s) 331
PspPI GGNCC 1 cut(s) 539
PvuII CAGCTG 1 cut(s) 574
RsaI GTAC 2 cut(s) 336, 501
RsaNI GTAC 2 cut(s) 335, 500
RseI CAYNNNNRTG 2 cut(s) 513, 525
SaqAI TTAA 1 cut(s) 279
SatI GCNGC 1 cut(s) 572
Sau3AI GATC 1 cut(s) 170
Sau96I GGNCC 1 cut(s) 539
SchI GAGTC 1 cut(s) 332
SduI GDGCHC 1 cut(s) 46
SetI ASST 9 cut(s) 41, 74, 161, 190, 227, 252, 264, 460, 576
SfaNI GCATC 5 cut(s) 26, 75, 330, 517, 591
SfcI CTRYAG 1 cut(s) 398
SinI GGWCC 1 cut(s) 539
SmiMI CAYNNNNRTG 2 cut(s) 513, 525
Sse9I AATT 4 cut(s) 10, 55, 272, 433
SsiI CCGC 2 cut(s) 147, 537
StyI CCWWGG 1 cut(s) 258
TaaI ACNGT 3 cut(s) 87, 117, 415
TaqI TCGA 4 cut(s) 82, 169, 437, 566
TasI AATT 4 cut(s) 10, 55, 272, 433
TatI WGTACW 2 cut(s) 334, 499
TfiI GAWTC 1 cut(s) 450
Tru1I TTAA 1 cut(s) 279
Tru9I TTAA 1 cut(s) 279
TscAI CASTG 1 cut(s) 301
TseFI GTSAC 1 cut(s) 218
TseI GCWGC 1 cut(s) 571
Tsp45I GTSAC 1 cut(s) 218
TspDTI ATGAA 3 cut(s) 170, 265, 285
TspGWI ACGGA 1 cut(s) 485
TspRI CASTG 1 cut(s) 301
VneI GTGCAC 1 cut(s) 42
VpaK11BI GGWCC 1 cut(s) 539
XapI RAATTY 2 cut(s) 272, 433
XmnI GAANNNNTTC 1 cut(s) 303
Zsp2I ATGCAT 1 cut(s) 532
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.