MD14G1063700.v1.1

OTU domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0000651
Physical Location & Seq
Reverse (-)
6739906 .. 6743232
3327 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1063700.v1.1.491

Sequence Viewer

Length: 1047 bp
ATGAAGTTAAATCTCGATTACCAAGGCTATGCGAATGCAATTGTTTGTCCTACCATTAACCATTCGCCGAAGACTACTGTCTGCCTGAGTGACTGTAACCTAATGCAGATGAGCAGTACGATCTGTAGTGTGGTGTCACGAGGAGCATCAAGTTCATGTTGTTACGCCCTGCAGACGGGGAAGTCGGGAACAAAGTTTGTCCGTTTGTCTAACTCCAAGACTCGTCCATCCAGTATTGGCCAAACATCCTATGCAAGCTGCTGCTTCAAATCTTTCTTCTCCAAGGAAATAGGCAACTCACGGTCATTAACAGTTAATGCTGGAGGGCCTCATAAGCGACGTTTTGACATTTCATTTACCTATCACGGTATGAAAACCAGGCTTTTGGTTTCAGGACAAGGAATGCTTCCCAAAATCAAGTGTAATGTGCGCCGTGTATCTTGCCCACAAGGATGCGCCTCTTCTGGCTTATTATTTGGGTTGTTGGTCTGTAATTGTTCTGAATCAGCGCATGCTCAAGCAGCTCACAAGGACAATAAGGAAGAGGACAGTGATTTGTCATATGTAAAATTTTCTCATGGGAAAAAGGTTTACACTGACTATTCTATCATTGGAATACCTGGTGACGGGAGGTGCTTATTCCGTTCAGTTGCTCATGGGGCTCATTTACGAGCTGGAAAATCAGCTCCCGGTGAGAGCCTTCAGAGAGAATTAGCGGATGGCTTGAGAGCTAGAGTTGCGGATGAATTTATCAAAAGGAGGGAAGAAACCGAATGGTTTGTTGAAGGCGATTTTGACACTTACGTTTCCCAAATAAGAAAGCCACATGTATGGGGAGGTGAGCCTGAGTTGTTCATGGCTTCACATGTTCTCCGGATGCCAATTACAGTATACATGTACGATGAGAAGGCCGGTGGCTTGATAACCATTGCCGAGTACGGGCAAGAATACGGCAGGGACAACCCGATCAGAGTTCTCTACCACGGCTTTGGCCATTATGATGCGTTGCAGATTCCCGGAAGGAGCGGAGGTAGATCGAGGCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
OTU

Protein Analysis

349

Amino Acids

38.35

Weight (kDa)

9.12

Isoelectric Point (pI)

51.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OTU PF02338 207 - 333 2.4e-28 OTU-like cysteine protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 181
AccBSI CCGCTC 1 cut(s) 1026
AccI GTMKAC 1 cut(s) 891
AccIII TCCGGA 1 cut(s) 873
AciI CCGC 3 cut(s) 716, 740, 1026
AcoI YGGCCR 2 cut(s) 238, 991
AcsI RAATTY 2 cut(s) 569, 746
AcuI CTGAAG 1 cut(s) 686
AfaI GTAC 3 cut(s) 118, 899, 938
AfiI CCNNNNNNNGG 3 cut(s) 175, 626, 939
AflIII ACRYGT 3 cut(s) 826, 865, 894
AgsI TTSAA 2 cut(s) 268, 785
AjnI CCWGG 2 cut(s) 377, 619
AluBI AGCT 5 cut(s) 258, 524, 674, 686, 731
AluI AGCT 5 cut(s) 258, 524, 674, 686, 731
Aor13HI TCCGGA 1 cut(s) 873
AoxI GGCC 4 cut(s) 238, 326, 909, 991
ApeKI GCWGC 3 cut(s) 258, 261, 521
ApoI RAATTY 2 cut(s) 569, 746
AspLEI GCGC 3 cut(s) 432, 458, 511
AspS9I GGNCC 1 cut(s) 326
AsuC2I CCSGG 2 cut(s) 690, 1017
AsuHPI GGTGA 3 cut(s) 635, 704, 851
BalI TGGCCA 2 cut(s) 240, 993
BanII GRGCYC 1 cut(s) 664
BauI CACGAG 1 cut(s) 138
BbsI GAAGAC 1 cut(s) 77
BbvI GCAGC 3 cut(s) 245, 248, 533
BccI CCATC 2 cut(s) 235, 713
BceAI ACGGC 3 cut(s) 417, 967, 1000
BciT130I CCWGG 2 cut(s) 379, 621
BcnI CCSGG 2 cut(s) 690, 1017
BfaI CTAG 1 cut(s) 732
BfmI CTRYAG 2 cut(s) 124, 170
BisI GCNGC 3 cut(s) 259, 262, 522
BlsI GCNGC 3 cut(s) 260, 263, 523
Bme1390I CCNGG 4 cut(s) 379, 621, 690, 1017
BmgT120I GGNCC 1 cut(s) 326
BmrFI CCNGG 4 cut(s) 379, 621, 690, 1017
BmsI GCATC 4 cut(s) 155, 443, 867, 991
BoxI GACNNNNGTC 1 cut(s) 77
BpiI GAAGAC 1 cut(s) 77
BpmI CTGGAG 1 cut(s) 342
BpuEI CTTGAG 2 cut(s) 501, 745
BpuMI CCSGG 2 cut(s) 690, 1017
BsaJI CCNNGG 3 cut(s) 22, 282, 982
BsaWI WCCGGW 1 cut(s) 873
BsaXI ACNNNNNCTCC 4 cut(s) 855, 885, 1015, 1045
Bsc4I CCNNNNNNNGG 3 cut(s) 175, 626, 939
Bse118I RCCGGY 1 cut(s) 911
Bse1I ACTGG 1 cut(s) 231
Bse3DI GCAATG 1 cut(s) 927
BseAI TCCGGA 1 cut(s) 873
BseBI CCWGG 2 cut(s) 379, 621
BseDI CCNNGG 3 cut(s) 22, 282, 982
BseGI GGATG 6 cut(s) 227, 245, 458, 724, 748, 882
BseLI CCNNNNNNNGG 3 cut(s) 175, 626, 939
BseMI GCAATG 1 cut(s) 927
BseMII CTCAG 2 cut(s) 77, 837
BseNI ACTGG 1 cut(s) 231
BseRI GAGGAG 1 cut(s) 156
BseXI GCAGC 3 cut(s) 245, 248, 533
BshFI GGCC 4 cut(s) 240, 328, 911, 993
BsiSI CCGG 4 cut(s) 690, 874, 912, 1017
BslFI GGGAC 1 cut(s) 971
BslI CCNNNNNNNGG 3 cut(s) 175, 626, 939
BsmFI GGGAC 1 cut(s) 971
BsmI GAATGC 2 cut(s) 40, 408
BsnI GGCC 4 cut(s) 240, 328, 911, 993
Bsp1286I GDGCHC 1 cut(s) 664
Bsp13I TCCGGA 1 cut(s) 873
Bsp143I GATC 3 cut(s) 120, 966, 1034
BspACI CCGC 3 cut(s) 716, 740, 1026
BspANI GGCC 4 cut(s) 240, 328, 911, 993
BspCNI CTCAG 2 cut(s) 78, 838
BspEI TCCGGA 1 cut(s) 873
BspMAI CTGCAG 1 cut(s) 174
BsrBI CCGCTC 1 cut(s) 1026
BsrDI GCAATG 1 cut(s) 927
BsrFI RCCGGY 1 cut(s) 911
BsrI ACTGG 1 cut(s) 231
BssAI RCCGGY 1 cut(s) 911
BssECI CCNNGG 3 cut(s) 22, 282, 982
BssMI GATC 3 cut(s) 120, 966, 1034
BssNAI GTATAC 1 cut(s) 892
BssSI CACGAG 1 cut(s) 138
BssT1I CCWWGG 2 cut(s) 22, 282
Bst1107I GTATAC 1 cut(s) 892
Bst2BI CACGAG 1 cut(s) 138
Bst2UI CCWGG 2 cut(s) 379, 621
Bst4CI ACNGT 7 cut(s) 79, 95, 303, 313, 368, 551, 889
Bst6I CTCTTC 2 cut(s) 466, 537
BstC8I GCNNGC 2 cut(s) 256, 513
BstDEI CTNAG 2 cut(s) 86, 846
BstDSI CCRYGG 1 cut(s) 982
BstF5I GGATG 6 cut(s) 227, 245, 458, 724, 748, 882
BstHHI GCGC 3 cut(s) 432, 458, 511
BstKTI GATC 3 cut(s) 123, 969, 1037
BstMBI GATC 3 cut(s) 120, 966, 1034
BstMWI GCNNNNNNNGC 4 cut(s) 334, 521, 659, 737
BstNI CCWGG 2 cut(s) 379, 621
BstNSI RCATGY 4 cut(s) 515, 830, 869, 898
BstPAI GACNNNNGTC 1 cut(s) 77
BstSCI CCNGG 4 cut(s) 377, 619, 688, 1015
BstSFI CTRYAG 2 cut(s) 124, 170
BstV1I GCAGC 3 cut(s) 245, 248, 533
BstV2I GAAGAC 1 cut(s) 77
BstXI CCANNNNNNTGG 3 cut(s) 385, 831, 989
BstZ17I GTATAC 1 cut(s) 892
BsuRI GGCC 4 cut(s) 240, 328, 911, 993
BtgI CCRYGG 1 cut(s) 982
BtsCI GGATG 6 cut(s) 227, 245, 458, 724, 748, 882
BtsIMutI CAGTG 2 cut(s) 556, 594
Cac8I GCNNGC 2 cut(s) 256, 513
CfoI GCGC 3 cut(s) 432, 458, 511
Cfr10I RCCGGY 1 cut(s) 911
Cfr13I GGNCC 1 cut(s) 326
CsiI ACCWGGT 1 cut(s) 619
Csp6I GTAC 3 cut(s) 117, 898, 937
CviAII CATG 8 cut(s) 156, 512, 578, 656, 827, 856, 866, 895
CviQI GTAC 3 cut(s) 117, 898, 937
DdeI CTNAG 2 cut(s) 86, 846
DpnI GATC 3 cut(s) 122, 968, 1036
DpnII GATC 3 cut(s) 120, 966, 1034
DrdI GACNNNNNNGTC 1 cut(s) 181
DseDI GACNNNNNNGTC 1 cut(s) 181
EaeI YGGCCR 2 cut(s) 238, 991
Eam1104I CTCTTC 2 cut(s) 466, 537
EarI CTCTTC 2 cut(s) 466, 537
Eco130I CCWWGG 2 cut(s) 22, 282
Eco24I GRGCYC 1 cut(s) 664
Eco57I CTGAAG 1 cut(s) 686
EcoO109I RGGNCCY 1 cut(s) 326
EcoRII CCWGG 2 cut(s) 377, 619
EcoT14I CCWWGG 2 cut(s) 22, 282
EcoT38I GRGCYC 1 cut(s) 664
ErhI CCWWGG 2 cut(s) 22, 282
FaeI CATG 8 cut(s) 159, 515, 581, 659, 830, 859, 869, 898
FalI AAGNNNNNCTT 2 cut(s) 390, 422
FaqI GGGAC 1 cut(s) 971
FatI CATG 8 cut(s) 155, 511, 577, 655, 826, 855, 865, 894
FauNDI CATATG 1 cut(s) 562
FblI GTMKAC 1 cut(s) 891
Fnu4HI GCNGC 3 cut(s) 259, 262, 522
FokI GGATG 6 cut(s) 214, 232, 465, 731, 755, 889
FriOI GRGCYC 1 cut(s) 664
Fsp4HI GCNGC 3 cut(s) 259, 262, 522
FspBI CTAG 1 cut(s) 732
GlaI GCGC 3 cut(s) 431, 457, 510
GluI GCNGC 3 cut(s) 259, 262, 522
GsuI CTGGAG 1 cut(s) 342
HaeIII GGCC 4 cut(s) 240, 328, 911, 993
HapII CCGG 4 cut(s) 690, 874, 912, 1017
HhaI GCGC 3 cut(s) 432, 458, 511
Hin1II CATG 8 cut(s) 159, 515, 581, 659, 830, 859, 869, 898
Hin6I GCGC 3 cut(s) 430, 456, 509
HinP1I GCGC 3 cut(s) 430, 456, 509
HinfI GANTC 3 cut(s) 220, 503, 1012
HpaII CCGG 4 cut(s) 690, 874, 912, 1017
HphI GGTGA 3 cut(s) 635, 704, 851
Hpy166II GTNNAC 2 cut(s) 592, 892
Hpy188I TCNGA 3 cut(s) 502, 705, 971
Hpy188III TCNNGA 5 cut(s) 14, 138, 186, 393, 874
Hpy8I GTNNAC 2 cut(s) 592, 892
Hpy99I CGWCG 1 cut(s) 342
HpyAV CCTTC 4 cut(s) 710, 779, 901, 1014
HpyCH4III ACNGT 7 cut(s) 79, 95, 303, 313, 368, 551, 889
HpyCH4IV ACGT 2 cut(s) 340, 804
HpyCH4V TGCA 5 cut(s) 38, 106, 172, 254, 1009
HpyF10VI GCNNNNNNNGC 4 cut(s) 334, 521, 659, 737
HpyF3I CTNAG 2 cut(s) 86, 846
HpySE526I ACGT 2 cut(s) 340, 804
Hsp92II CATG 8 cut(s) 159, 515, 581, 659, 830, 859, 869, 898
HspAI GCGC 3 cut(s) 430, 456, 509
Kpn2I TCCGGA 1 cut(s) 873
Kzo9I GATC 3 cut(s) 120, 966, 1034
LmnI GCTCC 3 cut(s) 143, 691, 1023
Lsp1109I GCAGC 3 cut(s) 245, 248, 533
LweI GCATC 4 cut(s) 155, 443, 867, 991
MabI ACCWGGT 1 cut(s) 619
MaeI CTAG 1 cut(s) 732
MaeII ACGT 2 cut(s) 340, 804
MaeIII GTNAC 5 cut(s) 89, 95, 135, 161, 623
MalI GATC 3 cut(s) 122, 968, 1036
MbiI CCGCTC 1 cut(s) 1026
MboI GATC 3 cut(s) 120, 966, 1034
MboII GAAGA 5 cut(s) 82, 268, 453, 554, 776
MfeI CAATTG 1 cut(s) 39
MhlI GDGCHC 1 cut(s) 664
MlsI TGGCCA 2 cut(s) 240, 993
MluCI AATT 6 cut(s) 39, 493, 569, 710, 746, 882
MluNI TGGCCA 2 cut(s) 240, 993
MlyI GAGTC 1 cut(s) 214
Mox20I TGGCCA 2 cut(s) 240, 993
MroI TCCGGA 1 cut(s) 873
MscI TGGCCA 2 cut(s) 240, 993
MseI TTAA 4 cut(s) 8, 57, 308, 315
MslI CAYNNNNRTG 3 cut(s) 451, 829, 999
Msp20I TGGCCA 2 cut(s) 240, 993
MspI CCGG 4 cut(s) 690, 874, 912, 1017
MspR9I CCNGG 4 cut(s) 379, 621, 690, 1017
MunI CAATTG 1 cut(s) 39
Mva1269I GAATGC 2 cut(s) 40, 408
MvaI CCWGG 2 cut(s) 379, 621
MwoI GCNNNNNNNGC 4 cut(s) 334, 521, 659, 737
NciI CCSGG 2 cut(s) 690, 1017
NdeI CATATG 1 cut(s) 562
NdeII GATC 3 cut(s) 120, 966, 1034
NlaIII CATG 8 cut(s) 159, 515, 581, 659, 830, 859, 869, 898
NmeAIII GCCGAG 1 cut(s) 958
NmuCI GTSAC 3 cut(s) 89, 135, 623
NspI RCATGY 4 cut(s) 515, 830, 869, 898
PaeI GCATGC 1 cut(s) 515
PciI ACATGT 3 cut(s) 826, 865, 894
PctI GAATGC 2 cut(s) 40, 408
PfeI GAWTC 2 cut(s) 503, 1012
PfoI TCCNGGA 1 cut(s) 1015
PkrI GCNGC 3 cut(s) 260, 263, 523
PleI GAGTC 1 cut(s) 214
PpsI GAGTC 1 cut(s) 214
PscI ACATGT 3 cut(s) 826, 865, 894
PshAI GACNNNNGTC 1 cut(s) 77
Psp6I CCWGG 2 cut(s) 377, 619
PspGI CCWGG 2 cut(s) 377, 619
PspPI GGNCC 1 cut(s) 326
PstI CTGCAG 1 cut(s) 174
RsaI GTAC 3 cut(s) 118, 899, 938
RsaNI GTAC 3 cut(s) 117, 898, 937
RseI CAYNNNNRTG 3 cut(s) 451, 829, 999
SaqAI TTAA 4 cut(s) 8, 57, 308, 315
SatI GCNGC 3 cut(s) 259, 262, 522
Sau3AI GATC 3 cut(s) 120, 966, 1034
Sau96I GGNCC 1 cut(s) 326
SchI GAGTC 1 cut(s) 214
ScrFI CCNGG 4 cut(s) 379, 621, 690, 1017
SduI GDGCHC 1 cut(s) 664
SexAI ACCWGGT 1 cut(s) 619
SfaNI GCATC 4 cut(s) 155, 443, 867, 991
SfcI CTRYAG 2 cut(s) 124, 170
SmiMI CAYNNNNRTG 3 cut(s) 451, 829, 999
SmlI CTYRAG 2 cut(s) 516, 724
SmoI CTYRAG 2 cut(s) 516, 724
SphI GCATGC 1 cut(s) 515
Sse9I AATT 6 cut(s) 39, 493, 569, 710, 746, 882
SsiI CCGC 3 cut(s) 716, 740, 1026
SspMI CTAG 1 cut(s) 732
StyD4I CCNGG 4 cut(s) 377, 619, 688, 1015
StyI CCWWGG 2 cut(s) 22, 282
TaaI ACNGT 7 cut(s) 79, 95, 303, 313, 368, 551, 889
TaiI ACGT 2 cut(s) 343, 807
TaqI TCGA 2 cut(s) 15, 1037
TasI AATT 6 cut(s) 39, 493, 569, 710, 746, 882
TfiI GAWTC 2 cut(s) 503, 1012
Tru1I TTAA 4 cut(s) 8, 57, 308, 315
Tru9I TTAA 4 cut(s) 8, 57, 308, 315
TscAI CASTG 2 cut(s) 556, 601
TseFI GTSAC 3 cut(s) 89, 135, 623
TseI GCWGC 3 cut(s) 258, 261, 521
Tsp45I GTSAC 3 cut(s) 89, 135, 623
TspDTI ATGAA 6 cut(s) 17, 144, 342, 386, 759, 844
TspGWI ACGGA 2 cut(s) 191, 632
TspRI CASTG 2 cut(s) 556, 601
XapI RAATTY 2 cut(s) 569, 746
XceI RCATGY 4 cut(s) 515, 830, 869, 898
XmiI GTMKAC 1 cut(s) 891
XspI CTAG 1 cut(s) 732
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.