FvH4_2g40191

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0002264
Physical Location & Seq
Forward (+)
28686202 .. 28687009
808 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g40191.t1

Sequence Viewer

Length: 594 bp
ATGACGATCTGGAAAACTATATGCAACCTCGTGCACTTTAGGGCACCAGCTATGCTTTCGATTGTTTCAGGATTCGTGAGGAAAATTATCTGCAATTTCCTTGGAGTACTGGTTCATCTGCGTATAGGAAAAAAAGATACAGCCGTCAAGATTAGCGGCCGAACTGAACTGGTGAAGCTCTGTGATCCTCCAACAACAGAAACGGTGGAAATCCTCATTGAACCCAATAATAACGATGCCGGTTGCAAAATAGAAGAACCAAATGCTTCCAAATACACTTATTGTACCATCTGCAAAAATCGTGATCACGACTTGGAGATGTGCCCTGACAGATTTGGGAAAGTTATAACAGTTTGTAGCGTCTGTGGTCATCTCCCATGTAAAAATGCGGCTGACGACCACAAGGAACACCATTATGTACGTCTACTATATTGTCGCCTTTGTGATGAGATTGGTGATCACTTGACTGCACTGTGCCCGGACATGGAATCCGACTTGGGTGACGAGGACTGGGATTCTGACACGGATGATGATAACTGGGATAATCAGGGTGATTTGCCGGAGAATACACAGACGGCCGCCGCTGCTGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

198

Amino Acids

22.02

Weight (kDa)

4.91

Isoelectric Point (pI)

30.39

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 347
AccB1I GGYRCC 1 cut(s) 43
AccI GTMKAC 1 cut(s) 424
AciI CCGC 4 cut(s) 156, 389, 579, 582
AclWI GGATC 1 cut(s) 179
AcoI YGGCCR 2 cut(s) 157, 576
AfaI GTAC 3 cut(s) 108, 286, 420
AfiI CCNNNNNNNGG 1 cut(s) 484
AgsI TTSAA 1 cut(s) 221
AloI GAACNNNNNNTCC 2 cut(s) 96, 128
AluBI AGCT 2 cut(s) 50, 178
AluI AGCT 2 cut(s) 50, 178
Alw21I GWGCWC 1 cut(s) 36
Alw44I GTGCAC 1 cut(s) 32
AlwI GGATC 1 cut(s) 179
AoxI GGCC 2 cut(s) 157, 576
ApaLI GTGCAC 1 cut(s) 32
ApeKI GCWGC 2 cut(s) 584, 587
AsuC2I CCSGG 1 cut(s) 479
AsuHPI GGTGA 4 cut(s) 184, 467, 512, 563
BaeGI GKGCMC 4 cut(s) 36, 46, 326, 479
BaeI ACNNNNGTAYC 2 cut(s) 129, 162
BanI GGYRCC 1 cut(s) 43
BauI CACGAG 1 cut(s) 29
Bbv12I GWGCWC 1 cut(s) 36
BbvI GCAGC 2 cut(s) 571, 574
BccI CCATC 1 cut(s) 296
BceAI ACGGC 2 cut(s) 128, 591
BclI TGATCA 2 cut(s) 304, 457
BcnI CCSGG 1 cut(s) 479
BisI GCNGC 6 cut(s) 157, 390, 579, 582, 585, 588
BlsI GCNGC 6 cut(s) 158, 391, 580, 583, 586, 589
BmcAI AGTACT 1 cut(s) 108
Bme1390I CCNGG 1 cut(s) 479
BmiI GGNNCC 1 cut(s) 45
BmrFI CCNGG 1 cut(s) 479
BmrI ACTGGG 2 cut(s) 520, 547
BmsI GCATC 1 cut(s) 226
BmuI ACTGGG 2 cut(s) 520, 547
BpuMI CCSGG 1 cut(s) 479
BsaJI CCNNGG 1 cut(s) 100
BsaXI ACNNNNNCTCC 2 cut(s) 96, 126
Bsc4I CCNNNNNNNGG 1 cut(s) 484
Bse118I RCCGGY 1 cut(s) 239
Bse1I ACTGG 4 cut(s) 114, 174, 515, 542
BseDI CCNNGG 1 cut(s) 100
BseGI GGATG 1 cut(s) 532
BseLI CCNNNNNNNGG 1 cut(s) 484
BseNI ACTGG 4 cut(s) 114, 174, 515, 542
BseSI GKGCMC 4 cut(s) 36, 46, 326, 479
BseX3I CGGCCG 2 cut(s) 157, 576
BseXI GCAGC 2 cut(s) 571, 574
BsgI GTGCAG 1 cut(s) 453
Bsh1285I CGRYCG 2 cut(s) 160, 579
BshFI GGCC 2 cut(s) 159, 578
BshNI GGYRCC 1 cut(s) 43
BsiEI CGRYCG 2 cut(s) 160, 579
BsiHKAI GWGCWC 1 cut(s) 36
BsiSI CCGG 3 cut(s) 240, 479, 560
BslI CCNNNNNNNGG 1 cut(s) 484
BsnI GGCC 2 cut(s) 159, 578
Bsp1286I GDGCHC 4 cut(s) 36, 46, 326, 479
Bsp143I GATC 4 cut(s) 6, 184, 304, 457
BspACI CCGC 4 cut(s) 156, 389, 579, 582
BspANI GGCC 2 cut(s) 159, 578
BspLI GGNNCC 1 cut(s) 45
BspPI GGATC 1 cut(s) 179
BspT107I GGYRCC 1 cut(s) 43
BsrFI RCCGGY 1 cut(s) 239
BsrI ACTGG 4 cut(s) 114, 174, 515, 542
BssAI RCCGGY 1 cut(s) 239
BssECI CCNNGG 1 cut(s) 100
BssMI GATC 4 cut(s) 6, 184, 304, 457
BssSI CACGAG 1 cut(s) 29
BssT1I CCWWGG 1 cut(s) 100
Bst2BI CACGAG 1 cut(s) 29
Bst4CI ACNGT 3 cut(s) 205, 352, 474
BstF5I GGATG 1 cut(s) 532
BstKTI GATC 4 cut(s) 9, 187, 307, 460
BstMBI GATC 4 cut(s) 6, 184, 304, 457
BstMCI CGRYCG 2 cut(s) 160, 579
BstMWI GCNNNNNNNGC 2 cut(s) 584, 587
BstSCI CCNGG 1 cut(s) 477
BstSLI GKGCMC 4 cut(s) 36, 46, 326, 479
BstV1I GCAGC 2 cut(s) 571, 574
BstZI CGGCCG 2 cut(s) 157, 576
BsuRI GGCC 2 cut(s) 159, 578
BtsCI GGATG 1 cut(s) 532
BtsIMutI CAGTG 1 cut(s) 470
Cfr10I RCCGGY 1 cut(s) 239
CseI GACGC 1 cut(s) 349
Csp6I GTAC 3 cut(s) 107, 285, 419
CviAII CATG 2 cut(s) 378, 484
CviJI RGCY 6 cut(s) 50, 143, 159, 178, 392, 578
CviKI_1 RGCY 6 cut(s) 50, 143, 159, 178, 392, 578
CviQI GTAC 3 cut(s) 107, 285, 419
DpnI GATC 4 cut(s) 8, 186, 306, 459
DpnII GATC 4 cut(s) 6, 184, 304, 457
EaeI YGGCCR 2 cut(s) 157, 576
EagI CGGCCG 2 cut(s) 157, 576
EclXI CGGCCG 2 cut(s) 157, 576
Eco130I CCWWGG 1 cut(s) 100
Eco52I CGGCCG 2 cut(s) 157, 576
EcoT14I CCWWGG 1 cut(s) 100
ErhI CCWWGG 1 cut(s) 100
FaeI CATG 2 cut(s) 381, 487
FatI CATG 2 cut(s) 377, 483
FbaI TGATCA 2 cut(s) 304, 457
FblI GTMKAC 1 cut(s) 424
Fnu4HI GCNGC 6 cut(s) 157, 390, 579, 582, 585, 588
FokI GGATG 1 cut(s) 539
Fsp4HI GCNGC 6 cut(s) 157, 390, 579, 582, 585, 588
GluI GCNGC 6 cut(s) 157, 390, 579, 582, 585, 588
HaeIII GGCC 2 cut(s) 159, 578
HapII CCGG 3 cut(s) 240, 479, 560
HgaI GACGC 1 cut(s) 349
Hin1II CATG 2 cut(s) 381, 487
HinfI GANTC 3 cut(s) 72, 488, 515
HpaII CCGG 3 cut(s) 240, 479, 560
HphI GGTGA 4 cut(s) 184, 467, 512, 563
Hpy166II GTNNAC 2 cut(s) 34, 425
Hpy188I TCNGA 2 cut(s) 493, 520
Hpy188III TCNNGA 6 cut(s) 10, 69, 76, 148, 302, 308
Hpy8I GTNNAC 2 cut(s) 34, 425
HpyCH4III ACNGT 3 cut(s) 205, 352, 474
HpyCH4IV ACGT 1 cut(s) 421
HpyCH4V TGCA 7 cut(s) 24, 34, 93, 246, 294, 470, 590
HpyF10VI GCNNNNNNNGC 2 cut(s) 584, 587
HpySE526I ACGT 1 cut(s) 421
Hsp92II CATG 2 cut(s) 381, 487
Ksp22I TGATCA 2 cut(s) 304, 457
Kzo9I GATC 4 cut(s) 6, 184, 304, 457
Lsp1109I GCAGC 2 cut(s) 571, 574
LweI GCATC 1 cut(s) 226
MaeII ACGT 1 cut(s) 421
MaeIII GTNAC 1 cut(s) 500
MalI GATC 4 cut(s) 8, 186, 306, 459
MboI GATC 4 cut(s) 6, 184, 304, 457
MboII GAAGA 1 cut(s) 266
MhlI GDGCHC 4 cut(s) 36, 46, 326, 479
MluCI AATT 2 cut(s) 84, 94
MmeI TCCRAC 2 cut(s) 215, 516
MnlI CCTC 5 cut(s) 38, 72, 198, 224, 499
MslI CAYNNNNRTG 1 cut(s) 414
MspA1I CMGCKG 1 cut(s) 584
MspI CCGG 3 cut(s) 240, 479, 560
MspR9I CCNGG 1 cut(s) 479
MwoI GCNNNNNNNGC 2 cut(s) 584, 587
NciI CCSGG 1 cut(s) 479
NdeII GATC 4 cut(s) 6, 184, 304, 457
NlaIII CATG 2 cut(s) 381, 487
NlaIV GGNNCC 1 cut(s) 45
NmuCI GTSAC 1 cut(s) 500
PfeI GAWTC 3 cut(s) 72, 488, 515
PkrI GCNGC 6 cut(s) 158, 391, 580, 583, 586, 589
PsiI TTATAA 1 cut(s) 347
PspN4I GGNNCC 1 cut(s) 45
RsaI GTAC 3 cut(s) 108, 286, 420
RsaNI GTAC 3 cut(s) 107, 285, 419
RseI CAYNNNNRTG 1 cut(s) 414
SatI GCNGC 6 cut(s) 157, 390, 579, 582, 585, 588
Sau3AI GATC 4 cut(s) 6, 184, 304, 457
ScaI AGTACT 1 cut(s) 108
ScrFI CCNGG 1 cut(s) 479
SduI GDGCHC 4 cut(s) 36, 46, 326, 479
SetI ASST 4 cut(s) 30, 52, 180, 424
SfaNI GCATC 1 cut(s) 226
SmiMI CAYNNNNRTG 1 cut(s) 414
Sse9I AATT 2 cut(s) 84, 94
SsiI CCGC 4 cut(s) 156, 389, 579, 582
StyD4I CCNGG 1 cut(s) 477
StyI CCWWGG 1 cut(s) 100
TaaI ACNGT 3 cut(s) 205, 352, 474
TaiI ACGT 1 cut(s) 424
TaqI TCGA 1 cut(s) 59
TasI AATT 2 cut(s) 84, 94
TatI WGTACW 1 cut(s) 106
TauI GCSGC 4 cut(s) 159, 392, 581, 584
TfiI GAWTC 3 cut(s) 72, 488, 515
TscAI CASTG 1 cut(s) 477
TseFI GTSAC 1 cut(s) 500
TseI GCWGC 2 cut(s) 584, 587
Tsp45I GTSAC 1 cut(s) 500
TspDTI ATGAA 1 cut(s) 104
TspGWI ACGGA 1 cut(s) 539
TspRI CASTG 1 cut(s) 477
VneI GTGCAC 1 cut(s) 32
XmiI GTMKAC 1 cut(s) 424
ZrmI AGTACT 1 cut(s) 108
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.