Rh5AG028100

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0002264
Physical Location & Seq
Forward (+)
1969036 .. 1969733
698 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG028100.1

Sequence Viewer

Length: 411 bp
ATGGATCTCACTATATCTACGTTCTCTCAACGCCGAAACGAAAAACCTAAACCCAACTTGTCCAGTCTCGGTCGTAATCGAAGACCTTCTCAGAGAAAGAGACTGCTTTACATTCAAATCGAACCAGCTATGAGTATCTGGATGATCTGCAACCAGGTGTACCATAAGGCTCCTGGTATGCTTTTTGTTTCAGGGTTCCTGAAGAAAATTATCCGCCACTTTCTTGGGCTGCTCCGAGTGTTTCGAGGAGAACAAGATACAGCCGCCAGCGCAATTGGCCAGCACCAGAAGCAGGCTCCGCCGGCGAAGCTTCATGATCCTCGACCGGGAGAACTCGAGGAGATTCTCATCGCTACTGGTTTTCAATATTTGAAAATTCTAAAAGACTGCAAGATTACTATCTTATATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

136

Amino Acids

15.67

Weight (kDa)

10.17

Isoelectric Point (pI)

54.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 214, 264, 299
AclWI GGATC 2 cut(s) 12, 311
AcoI YGGCCR 1 cut(s) 277
AcsI RAATTY 1 cut(s) 375
AcuI CTGAAG 1 cut(s) 221
AfaI GTAC 1 cut(s) 161
AfiI CCNNNNNNNGG 2 cut(s) 292, 326
AgsI TTSAA 3 cut(s) 116, 365, 373
AjnI CCWGG 2 cut(s) 153, 172
AluBI AGCT 2 cut(s) 128, 310
AluI AGCT 2 cut(s) 128, 310
Alw26I GTCTC 2 cut(s) 71, 94
AlwI GGATC 2 cut(s) 12, 311
Ama87I CYCGRG 1 cut(s) 335
AoxI GGCC 1 cut(s) 277
ApeKI GCWGC 1 cut(s) 229
ApoI RAATTY 1 cut(s) 375
Asp700I GAANNNNTTC 1 cut(s) 85
AspLEI GCGC 1 cut(s) 272
AsuC2I CCSGG 1 cut(s) 327
AvaI CYCGRG 1 cut(s) 335
BalI TGGCCA 1 cut(s) 279
BbsI GAAGAC 1 cut(s) 88
BbvI GCAGC 1 cut(s) 216
BciT130I CCWGG 2 cut(s) 155, 174
BcnI CCSGG 1 cut(s) 327
BcoDI GTCTC 2 cut(s) 71, 94
BisI GCNGC 2 cut(s) 230, 264
BlsI GCNGC 2 cut(s) 231, 265
Bme1390I CCNGG 3 cut(s) 155, 174, 327
BmeT110I CYCGRG 1 cut(s) 335
BmiI GGNNCC 3 cut(s) 171, 197, 297
BmrFI CCNGG 3 cut(s) 155, 174, 327
BpiI GAAGAC 1 cut(s) 88
BpuMI CCSGG 1 cut(s) 327
BsaBI GATNNNNATC 2 cut(s) 347, 398
Bsc4I CCNNNNNNNGG 2 cut(s) 292, 326
Bse118I RCCGGY 1 cut(s) 301
Bse1I ACTGG 2 cut(s) 63, 361
Bse8I GATNNNNATC 2 cut(s) 347, 398
BseBI CCWGG 2 cut(s) 155, 174
BseGI GGATG 1 cut(s) 147
BseJI GATNNNNATC 2 cut(s) 347, 398
BseLI CCNNNNNNNGG 2 cut(s) 292, 326
BseMII CTCAG 1 cut(s) 104
BseNI ACTGG 2 cut(s) 63, 361
BseRI GAGGAG 2 cut(s) 261, 353
BseXI GCAGC 1 cut(s) 216
Bsh1285I CGRYCG 2 cut(s) 73, 326
BshFI GGCC 1 cut(s) 279
BsiEI CGRYCG 2 cut(s) 73, 326
BsiHKCI CYCGRG 1 cut(s) 335
BsiSI CCGG 2 cut(s) 302, 326
BslI CCNNNNNNNGG 2 cut(s) 292, 326
BsmAI GTCTC 2 cut(s) 71, 94
BsnI GGCC 1 cut(s) 279
BsoBI CYCGRG 1 cut(s) 335
Bsp143I GATC 3 cut(s) 4, 144, 316
BspACI CCGC 3 cut(s) 214, 264, 299
BspANI GGCC 1 cut(s) 279
BspCNI CTCAG 1 cut(s) 103
BspHI TCATGA 1 cut(s) 313
BspLI GGNNCC 3 cut(s) 171, 197, 297
BspPI GGATC 2 cut(s) 12, 311
BsrFI RCCGGY 1 cut(s) 301
BsrI ACTGG 2 cut(s) 63, 361
BssAI RCCGGY 1 cut(s) 301
BssMI GATC 3 cut(s) 4, 144, 316
Bst2UI CCWGG 2 cut(s) 155, 174
BstC8I GCNNGC 4 cut(s) 268, 281, 294, 303
BstDEI CTNAG 1 cut(s) 90
BstF5I GGATG 1 cut(s) 147
BstHHI GCGC 1 cut(s) 272
BstKTI GATC 3 cut(s) 7, 147, 319
BstMAI GTCTC 2 cut(s) 71, 94
BstMBI GATC 3 cut(s) 4, 144, 316
BstMCI CGRYCG 2 cut(s) 73, 326
BstMWI GCNNNNNNNGC 6 cut(s) 269, 276, 289, 298, 302, 307
BstNI CCWGG 2 cut(s) 155, 174
BstSCI CCNGG 3 cut(s) 153, 172, 325
BstV1I GCAGC 1 cut(s) 216
BstV2I GAAGAC 1 cut(s) 88
BstX2I RGATCY 1 cut(s) 4
BstXI CCANNNNNNTGG 1 cut(s) 224
BstYI RGATCY 1 cut(s) 4
BsuRI GGCC 1 cut(s) 279
BtgZI GCGATG 1 cut(s) 334
BtsCI GGATG 1 cut(s) 147
Cac8I GCNNGC 4 cut(s) 268, 281, 294, 303
CciI TCATGA 1 cut(s) 313
CfoI GCGC 1 cut(s) 272
Cfr10I RCCGGY 1 cut(s) 301
CsiI ACCWGGT 1 cut(s) 153
Csp6I GTAC 1 cut(s) 160
CviAII CATG 1 cut(s) 314
CviJI RGCY 7 cut(s) 128, 170, 229, 263, 279, 296, 310
CviKI_1 RGCY 7 cut(s) 128, 170, 229, 263, 279, 296, 310
CviQI GTAC 1 cut(s) 160
DdeI CTNAG 1 cut(s) 90
DpnI GATC 3 cut(s) 6, 146, 318
DpnII GATC 3 cut(s) 4, 144, 316
EaeI YGGCCR 1 cut(s) 277
EciI GGCGGA 2 cut(s) 203, 288
Eco57I CTGAAG 1 cut(s) 221
Eco88I CYCGRG 1 cut(s) 335
EcoRII CCWGG 2 cut(s) 153, 172
FaeI CATG 1 cut(s) 317
FaiI YATR 6 cut(s) 14, 131, 165, 179, 315, 406
FatI CATG 1 cut(s) 313
Fnu4HI GCNGC 2 cut(s) 230, 264
FokI GGATG 1 cut(s) 154
Fsp4HI GCNGC 2 cut(s) 230, 264
GlaI GCGC 1 cut(s) 271
GluI GCNGC 2 cut(s) 230, 264
HaeIII GGCC 1 cut(s) 279
HapII CCGG 2 cut(s) 302, 326
HhaI GCGC 1 cut(s) 272
Hin1II CATG 1 cut(s) 317
Hin6I GCGC 1 cut(s) 270
HinP1I GCGC 1 cut(s) 270
HindIII AAGCTT 1 cut(s) 308
HinfI GANTC 1 cut(s) 343
HpaII CCGG 2 cut(s) 302, 326
Hpy166II GTNNAC 1 cut(s) 160
Hpy188I TCNGA 2 cut(s) 93, 236
Hpy188III TCNNGA 3 cut(s) 139, 199, 314
Hpy8I GTNNAC 1 cut(s) 160
HpyAV CCTTC 1 cut(s) 96
HpyCH4IV ACGT 1 cut(s) 20
HpyCH4V TGCA 2 cut(s) 150, 390
HpyF10VI GCNNNNNNNGC 6 cut(s) 269, 276, 289, 298, 302, 307
HpyF3I CTNAG 1 cut(s) 90
HpySE526I ACGT 1 cut(s) 20
Hsp92II CATG 1 cut(s) 317
HspAI GCGC 1 cut(s) 270
KroI GCCGGC 1 cut(s) 301
KroNI GCCGGC 1 cut(s) 303
Kzo9I GATC 3 cut(s) 4, 144, 316
LmnI GCTCC 3 cut(s) 175, 237, 301
Lsp1109I GCAGC 1 cut(s) 216
MabI ACCWGGT 1 cut(s) 153
MaeII ACGT 1 cut(s) 20
MalI GATC 3 cut(s) 6, 146, 318
MboI GATC 3 cut(s) 4, 144, 316
MboII GAAGA 2 cut(s) 93, 214
MfeI CAATTG 1 cut(s) 273
MflI RGATCY 1 cut(s) 4
MlsI TGGCCA 1 cut(s) 279
MluCI AATT 3 cut(s) 207, 273, 375
MluNI TGGCCA 1 cut(s) 279
MnlI CCTC 3 cut(s) 239, 330, 331
Mox20I TGGCCA 1 cut(s) 279
MreI CGCCGGCG 1 cut(s) 301
MroNI GCCGGC 1 cut(s) 301
MroXI GAANNNNTTC 1 cut(s) 85
MscI TGGCCA 1 cut(s) 279
Msp20I TGGCCA 1 cut(s) 279
MspI CCGG 2 cut(s) 302, 326
MspR9I CCNGG 3 cut(s) 155, 174, 327
MunI CAATTG 1 cut(s) 273
MvaI CCWGG 2 cut(s) 155, 174
MwoI GCNNNNNNNGC 6 cut(s) 269, 276, 289, 298, 302, 307
NaeI GCCGGC 1 cut(s) 303
NciI CCSGG 1 cut(s) 327
NdeII GATC 3 cut(s) 4, 144, 316
NgoMIV GCCGGC 1 cut(s) 301
NlaIII CATG 1 cut(s) 317
NlaIV GGNNCC 3 cut(s) 171, 197, 297
PaeR7I CTCGAG 1 cut(s) 335
PagI TCATGA 1 cut(s) 313
PdiI GCCGGC 1 cut(s) 303
PdmI GAANNNNTTC 1 cut(s) 85
PfeI GAWTC 1 cut(s) 343
PkrI GCNGC 2 cut(s) 231, 265
Psp6I CCWGG 2 cut(s) 153, 172
PspGI CCWGG 2 cut(s) 153, 172
PspN4I GGNNCC 3 cut(s) 171, 197, 297
PspXI VCTCGAGB 1 cut(s) 335
PsuI RGATCY 1 cut(s) 4
RsaI GTAC 1 cut(s) 161
RsaNI GTAC 1 cut(s) 160
SatI GCNGC 2 cut(s) 230, 264
Sau3AI GATC 3 cut(s) 4, 144, 316
ScrFI CCNGG 3 cut(s) 155, 174, 327
SetI ASST 6 cut(s) 23, 49, 88, 130, 159, 312
SexAI ACCWGGT 1 cut(s) 153
Sfr274I CTCGAG 1 cut(s) 335
SgrAI CRCCGGYG 1 cut(s) 301
SlaI CTCGAG 1 cut(s) 335
SmlI CTYRAG 1 cut(s) 335
SmoI CTYRAG 1 cut(s) 335
Sse9I AATT 3 cut(s) 207, 273, 375
SsiI CCGC 3 cut(s) 214, 264, 299
SspI AATATT 1 cut(s) 368
StyD4I CCNGG 3 cut(s) 153, 172, 325
TaiI ACGT 1 cut(s) 23
TaqI TCGA 5 cut(s) 79, 120, 244, 322, 336
TaqII GACCGA 1 cut(s) 59
TasI AATT 3 cut(s) 207, 273, 375
TauI GCSGC 1 cut(s) 266
TfiI GAWTC 1 cut(s) 343
TseI GCWGC 1 cut(s) 229
TspDTI ATGAA 1 cut(s) 302
XapI RAATTY 1 cut(s) 375
XcmI CCANNNNNNNNNTGG 1 cut(s) 170
XhoI CTCGAG 1 cut(s) 335
XmnI GAANNNNTTC 1 cut(s) 85
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.