Rh5CG433600

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0002264
Physical Location & Seq
Reverse (-)
60032245 .. 60033102
858 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG433600.1

Sequence Viewer

Length: 546 bp
ATGTTATTATTCTCTTCTTTACGCCAAACCTTAAGTCCTAACCTAAAGCACCAAATTTCTCTCCTTTACACAGATGCAGCCGATATGAGTATTAGCCCAACTGGACAGCAGGACCTGGAACAACTGGTGAAACTCTGTGATCCACCAACAACAGAATTAGAGGAAATCCTCATCCAGTCCACTAACGCTGCTGGTTGCAAGGTAGAAGAACCAAAGGCTTCCGATTACACGTATTATTGTAGGATTTGCGATGAGAATTGTGATCACAGCACTGAGACGTGCCCTGACAGATCTGGAAAATTTATAACAGTTTGTAGGGTTTGTGACTGTCTCCCATGTAAAAATGAGGCTGACCACAAGGAAGACCATTATGAACGGTTCTTTTTTTGTCATCTTTGTAGTACGATTGGTCAGCACTTGACTGAAATGTGCCCTGACATGGATTCCGACATGGGTGACGAGGACTGGGGTTCTGACATGGATGACGAGAACAGGGAGAAGGGTGATTTTCCGGAGAATACACACAGCGCCGCCAATGCTGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

181

Amino Acids

20.42

Weight (kDa)

4.38

Isoelectric Point (pI)

50.09

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 305
AccIII TCCGGA 1 cut(s) 511
AciI CCGC 1 cut(s) 531
AclWI GGATC 1 cut(s) 134
AcsI RAATTY 2 cut(s) 54, 299
AfaI GTAC 1 cut(s) 403
AfiI CCNNNNNNNGG 1 cut(s) 439
AflII CTTAAG 1 cut(s) 31
AflIII ACRYGT 1 cut(s) 228
AjiI CACGTC 1 cut(s) 279
AjnI CCWGG 1 cut(s) 114
Alw26I GTCTC 2 cut(s) 269, 335
AlwI GGATC 1 cut(s) 134
AlwNI CAGNNNCTG 1 cut(s) 115
Aor13HI TCCGGA 1 cut(s) 511
ApeKI GCWGC 3 cut(s) 77, 188, 539
ApoI RAATTY 2 cut(s) 54, 299
ArsI GACNNNNNNTTYG 2 cut(s) 19, 51
AspLEI GCGC 1 cut(s) 530
AspS9I GGNCC 1 cut(s) 112
AsuHPI GGTGA 3 cut(s) 139, 467, 515
AvaII GGWCC 1 cut(s) 112
BaeGI GKGCMC 2 cut(s) 284, 434
BbsI GAAGAC 1 cut(s) 369
BbvI GCAGC 3 cut(s) 89, 175, 526
BciT130I CCWGG 1 cut(s) 116
BclI TGATCA 1 cut(s) 262
BcoDI GTCTC 2 cut(s) 269, 335
BfoI RGCGCY 1 cut(s) 531
BfrI CTTAAG 1 cut(s) 31
BglII AGATCT 1 cut(s) 290
BisI GCNGC 4 cut(s) 78, 189, 531, 540
BlsI GCNGC 4 cut(s) 79, 190, 532, 541
Bme1390I CCNGG 1 cut(s) 116
Bme18I GGWCC 1 cut(s) 112
BmgBI CACGTC 1 cut(s) 279
BmgT120I GGNCC 1 cut(s) 112
BmrFI CCNGG 1 cut(s) 116
BmrI ACTGGG 1 cut(s) 475
BmsI GCATC 1 cut(s) 64
BmuI ACTGGG 1 cut(s) 475
BpiI GAAGAC 1 cut(s) 369
BsaAI YACGTR 1 cut(s) 231
BsaWI WCCGGW 1 cut(s) 511
Bsc4I CCNNNNNNNGG 1 cut(s) 439
Bse1I ACTGG 4 cut(s) 106, 129, 175, 470
BseAI TCCGGA 1 cut(s) 511
BseBI CCWGG 1 cut(s) 116
BseGI GGATG 2 cut(s) 171, 487
BseLI CCNNNNNNNGG 1 cut(s) 439
BseMII CTCAG 1 cut(s) 264
BseNI ACTGG 4 cut(s) 106, 129, 175, 470
BseSI GKGCMC 2 cut(s) 284, 434
BseXI GCAGC 3 cut(s) 89, 175, 526
BsiSI CCGG 1 cut(s) 512
BslI CCNNNNNNNGG 1 cut(s) 439
BsmAI GTCTC 2 cut(s) 269, 335
BsmBI CGTCTC 1 cut(s) 269
Bsp1286I GDGCHC 2 cut(s) 284, 434
Bsp13I TCCGGA 1 cut(s) 511
Bsp143I GATC 3 cut(s) 139, 262, 290
BspACI CCGC 1 cut(s) 531
BspCNI CTCAG 1 cut(s) 265
BspEI TCCGGA 1 cut(s) 511
BspPI GGATC 1 cut(s) 134
BspTI CTTAAG 1 cut(s) 31
BsrI ACTGG 4 cut(s) 106, 129, 175, 470
BssMI GATC 3 cut(s) 139, 262, 290
Bst2UI CCWGG 1 cut(s) 116
Bst4CI ACNGT 3 cut(s) 310, 329, 378
Bst6I CTCTTC 1 cut(s) 19
BstAFI CTTAAG 1 cut(s) 31
BstBAI YACGTR 1 cut(s) 231
BstDEI CTNAG 1 cut(s) 273
BstF5I GGATG 2 cut(s) 171, 487
BstH2I RGCGCY 1 cut(s) 531
BstHHI GCGC 1 cut(s) 530
BstKTI GATC 3 cut(s) 142, 265, 293
BstMAI GTCTC 2 cut(s) 269, 335
BstMBI GATC 3 cut(s) 139, 262, 290
BstMWI GCNNNNNNNGC 2 cut(s) 536, 539
BstNI CCWGG 1 cut(s) 116
BstSCI CCNGG 1 cut(s) 114
BstSLI GKGCMC 2 cut(s) 284, 434
BstV1I GCAGC 3 cut(s) 89, 175, 526
BstV2I GAAGAC 1 cut(s) 369
BstX2I RGATCY 1 cut(s) 290
BstYI RGATCY 1 cut(s) 290
BtgZI GCGATG 1 cut(s) 264
BtrI CACGTC 1 cut(s) 279
BtsCI GGATG 2 cut(s) 171, 487
BtsIMutI CAGTG 1 cut(s) 270
CaiI CAGNNNCTG 1 cut(s) 115
CfoI GCGC 1 cut(s) 530
Cfr13I GGNCC 1 cut(s) 112
Csp6I GTAC 1 cut(s) 402
CviAII CATG 4 cut(s) 336, 439, 451, 478
CviJI RGCY 4 cut(s) 80, 96, 218, 350
CviKI_1 RGCY 4 cut(s) 80, 96, 218, 350
CviQI GTAC 1 cut(s) 402
DdeI CTNAG 1 cut(s) 273
DpnI GATC 3 cut(s) 141, 264, 292
DpnII GATC 3 cut(s) 139, 262, 290
Eam1104I CTCTTC 1 cut(s) 19
EarI CTCTTC 1 cut(s) 19
Eco47I GGWCC 1 cut(s) 112
EcoO109I RGGNCCY 1 cut(s) 112
EcoRII CCWGG 1 cut(s) 114
Esp3I CGTCTC 1 cut(s) 269
FaeI CATG 4 cut(s) 339, 442, 454, 481
FaiI YATR 8 cut(s) 86, 305, 337, 372, 440, 452, 479, 544
FatI CATG 4 cut(s) 335, 438, 450, 477
FbaI TGATCA 1 cut(s) 262
Fnu4HI GCNGC 4 cut(s) 78, 189, 531, 540
FokI GGATG 2 cut(s) 158, 494
Fsp4HI GCNGC 4 cut(s) 78, 189, 531, 540
GlaI GCGC 1 cut(s) 529
GluI GCNGC 4 cut(s) 78, 189, 531, 540
HaeII RGCGCY 1 cut(s) 531
HapII CCGG 1 cut(s) 512
HhaI GCGC 1 cut(s) 530
Hin1II CATG 4 cut(s) 339, 442, 454, 481
Hin6I GCGC 1 cut(s) 528
HinP1I GCGC 1 cut(s) 528
HinfI GANTC 1 cut(s) 443
HpaII CCGG 1 cut(s) 512
HphI GGTGA 3 cut(s) 139, 467, 515
Hpy166II GTNNAC 1 cut(s) 180
Hpy188I TCNGA 3 cut(s) 223, 448, 475
Hpy188III TCNNGA 2 cut(s) 294, 512
Hpy8I GTNNAC 1 cut(s) 180
HpyAV CCTTC 1 cut(s) 493
HpyCH4III ACNGT 3 cut(s) 310, 329, 378
HpyCH4IV ACGT 2 cut(s) 230, 278
HpyCH4V TGCA 3 cut(s) 77, 198, 542
HpyF10VI GCNNNNNNNGC 2 cut(s) 536, 539
HpyF3I CTNAG 1 cut(s) 273
HpySE526I ACGT 2 cut(s) 230, 278
Hsp92II CATG 4 cut(s) 339, 442, 454, 481
HspAI GCGC 1 cut(s) 528
Kpn2I TCCGGA 1 cut(s) 511
Ksp22I TGATCA 1 cut(s) 262
Kzo9I GATC 3 cut(s) 139, 262, 290
Lsp1109I GCAGC 3 cut(s) 89, 175, 526
LweI GCATC 1 cut(s) 64
MaeII ACGT 2 cut(s) 230, 278
MaeIII GTNAC 2 cut(s) 323, 455
MalI GATC 3 cut(s) 141, 264, 292
MboI GATC 3 cut(s) 139, 262, 290
MboII GAAGA 3 cut(s) 6, 218, 374
MflI RGATCY 1 cut(s) 290
MhlI GDGCHC 2 cut(s) 284, 434
MluCI AATT 4 cut(s) 54, 155, 256, 299
MmeI TCCRAC 1 cut(s) 471
MnlI CCTC 4 cut(s) 154, 179, 340, 454
MroI TCCGGA 1 cut(s) 511
MseI TTAA 1 cut(s) 32
MspCI CTTAAG 1 cut(s) 31
MspI CCGG 1 cut(s) 512
MspR9I CCNGG 1 cut(s) 116
MvaI CCWGG 1 cut(s) 116
MwoI GCNNNNNNNGC 2 cut(s) 536, 539
NdeII GATC 3 cut(s) 139, 262, 290
NlaIII CATG 4 cut(s) 339, 442, 454, 481
NmuCI GTSAC 2 cut(s) 323, 455
PfeI GAWTC 1 cut(s) 443
PkrI GCNGC 4 cut(s) 79, 190, 532, 541
Ppu21I YACGTR 1 cut(s) 231
PpuMI RGGWCCY 1 cut(s) 112
PsiI TTATAA 1 cut(s) 305
Psp5II RGGWCCY 1 cut(s) 112
Psp6I CCWGG 1 cut(s) 114
PspGI CCWGG 1 cut(s) 114
PspPI GGNCC 1 cut(s) 112
PspPPI RGGWCCY 1 cut(s) 112
PstNI CAGNNNCTG 1 cut(s) 115
PsuI RGATCY 1 cut(s) 290
RsaI GTAC 1 cut(s) 403
RsaNI GTAC 1 cut(s) 402
SaqAI TTAA 1 cut(s) 32
SatI GCNGC 4 cut(s) 78, 189, 531, 540
Sau3AI GATC 3 cut(s) 139, 262, 290
Sau96I GGNCC 1 cut(s) 112
ScrFI CCNGG 1 cut(s) 116
SduI GDGCHC 2 cut(s) 284, 434
SetI ASST 6 cut(s) 32, 45, 117, 204, 233, 281
SfaNI GCATC 1 cut(s) 64
SinI GGWCC 1 cut(s) 112
SmlI CTYRAG 1 cut(s) 31
SmoI CTYRAG 1 cut(s) 31
Sse9I AATT 4 cut(s) 54, 155, 256, 299
SsiI CCGC 1 cut(s) 531
StyD4I CCNGG 1 cut(s) 114
TaaI ACNGT 3 cut(s) 310, 329, 378
TaiI ACGT 2 cut(s) 233, 281
TasI AATT 4 cut(s) 54, 155, 256, 299
TauI GCSGC 1 cut(s) 533
TfiI GAWTC 1 cut(s) 443
Tru1I TTAA 1 cut(s) 32
Tru9I TTAA 1 cut(s) 32
TscAI CASTG 1 cut(s) 277
TseFI GTSAC 2 cut(s) 323, 455
TseI GCWGC 3 cut(s) 77, 188, 539
Tsp45I GTSAC 2 cut(s) 323, 455
TspDTI ATGAA 1 cut(s) 387
TspRI CASTG 1 cut(s) 277
Vha464I CTTAAG 1 cut(s) 31
VpaK11BI GGWCC 1 cut(s) 112
XapI RAATTY 2 cut(s) 54, 299
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.