Rorug04G0396300

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0002264
Physical Location & Seq
Forward (+)
56276154 .. 56278557
2404 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0396300.1

Sequence Viewer

Length: 1269 bp
ATGCAAGTCCTGCTCGGAGATGGAATCTTCAATTCAGATGGAGAAGTTTGGAGGAAACAGAGAAAGACTGCAAGCTTCGAATTTGCATCCAAGAATTTGAGGGACTTCAGCACTGTTGTCTTCAGAGATTACAGCCTAAAGCTCCATGGCATTCTGAGTCAAGCATCTTTTCAAGGGCAACAAGTAGACATGCAGGAATTGCTGATGAGGATGACTCTGGACTCCATATGTAAGGTGGGATTTGGTGTAGAGATTGGAACCCTGGCTCCAGATCTACCAGATAATCAGTTTGCCCAGGCCTTCGACACTGCAAACATAATTGTCACATATCGATTCATTGATCCACTATGGAAAATAAAGAAATTCTTAAATTGGGGATCAGAAGCTTTACTTGACAAGAGCATGAAAACCATAGATGATTTTACATATTCTGTAATTCGGAAAAGGAAATCAGAGATAAAAGAAGCTCAACAGAGTTCAGATAAAAACCTGATGAAGCATGATATACTGTCAAGATTCATCGAGTTAGGTGAAGACCCAGAAAGCAACTTAACTGACAAAAGCCTCAGGGATGTTGTCCTCAACTTCGTAATAGCTGGTCGGGACACAACAGCAACCACTCTCTCATGGGCGATTTACATGATAATGACCCATCCCGAGGTAGCCGAGAAGCTATATACGGAGCTTAAGACTCTTGAAGAAGAACAAGCACGAGAAGAGAAAGATTCATTACTCCAATATGACAAAGAGGACCTTGAATCATTCAATCAAAGAGTGAAACAATTTGCAGGACTCATGAACTATGATTCATTGGGGAGACTCTATTATTTGCATGCAGTCATCACAGAGACACTCCGTCTGTACCCTGCAGTCCCTCAGGACCCTAAAGGCATATTGGAAGATGATGTTCTACCAGATGGAACAAAAGTCAGAGCAGGAGGAATGGTAACCTACGTTCCCTATTCGATGGGTAGAATGGAGTACAATTGGGGTGCTGATGCCGCTTCATTTAAGCCAGAGAGATGGCTCAAAGACGGTTACTTCTTAAATGCATCGCCATTTAAGTTCACCGCATTTCAGGCAGGACCAAGAATATGCCTAGGGAAGGACTCTGCATACCTCCAAATGAAGATGGTACTGGCCATTCTGTGCAGATTTTTCACATTCACTTTGGTTCCCGGTCATCCAGTTGAGTATAGGATGATGACAATTTTATCAATGAAGCATGGCTTGAAGCTTACAGTAGCCAGACGCTCATCAGATGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

422

Amino Acids

48.29

Weight (kDa)

6.15

Isoelectric Point (pI)

34.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 5 - 411 1.9e-64 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 186
AciI CCGC 2 cut(s) 1002, 1071
AclWI GGATC 2 cut(s) 335, 385
AcoI YGGCCR 1 cut(s) 1140
AcsI RAATTY 3 cut(s) 80, 94, 362
AcuI CTGAAG 2 cut(s) 91, 106
AfaI GTAC 3 cut(s) 863, 983, 1137
AfiI CCNNNNNNNGG 2 cut(s) 658, 1105
AflII CTTAAG 1 cut(s) 686
AgsI TTSAA 6 cut(s) 31, 173, 698, 758, 766, 1234
AhdI GACNNNNNGTC 1 cut(s) 855
AjnI CCWGG 2 cut(s) 261, 294
AloI GAACNNNNNNTCC 2 cut(s) 250, 282
AluBI AGCT 8 cut(s) 75, 142, 386, 467, 596, 673, 685, 1237
AluI AGCT 8 cut(s) 75, 142, 386, 467, 596, 673, 685, 1237
Alw26I GTCTC 2 cut(s) 811, 842
AlwI GGATC 2 cut(s) 335, 385
Ama87I CYCGRG 1 cut(s) 656
AoxI GGCC 2 cut(s) 297, 1140
ApoI RAATTY 3 cut(s) 80, 94, 362
AspA2I CCTAGG 1 cut(s) 1099
AspS9I GGNCC 3 cut(s) 751, 880, 1085
AsuC2I CCSGG 1 cut(s) 1179
AsuHPI GGTGA 2 cut(s) 542, 1060
AsuII TTCGAA 1 cut(s) 78
AvaI CYCGRG 1 cut(s) 656
AvaII GGWCC 3 cut(s) 751, 880, 1085
AvrII CCTAGG 1 cut(s) 1099
AxyI CCTNAGG 2 cut(s) 566, 876
BalI TGGCCA 1 cut(s) 1142
BauI CACGAG 1 cut(s) 711
BbsI GAAGAC 2 cut(s) 112, 540
BccI CCATC 7 cut(s) 14, 32, 660, 911, 961, 1017, 1126
BciT130I CCWGG 2 cut(s) 263, 296
BcnI CCSGG 1 cut(s) 1179
BcoDI GTCTC 2 cut(s) 811, 842
BfaI CTAG 1 cut(s) 1100
BfmI CTRYAG 1 cut(s) 867
BfrI CTTAAG 1 cut(s) 686
BglII AGATCT 1 cut(s) 271
BisI GCNGC 1 cut(s) 1002
BlnI CCTAGG 1 cut(s) 1099
BlsI GCNGC 1 cut(s) 1003
Bme1390I CCNGG 3 cut(s) 263, 296, 1179
Bme18I GGWCC 3 cut(s) 751, 880, 1085
BmeRI GACNNNNNGTC 1 cut(s) 855
BmeT110I CYCGRG 1 cut(s) 656
BmgT120I GGNCC 3 cut(s) 751, 880, 1085
BmiI GGNNCC 4 cut(s) 259, 267, 882, 1176
BmrFI CCNGG 3 cut(s) 263, 296, 1179
BmsI GCATC 5 cut(s) 95, 173, 988, 1061, 1252
BpiI GAAGAC 2 cut(s) 112, 540
BplI GAGNNNNNCTC 2 cut(s) 199, 231
BpmI CTGGAG 1 cut(s) 252
Bpu14I TTCGAA 1 cut(s) 78
BpuMI CCSGG 1 cut(s) 1179
Bsa29I ATCGAT 1 cut(s) 331
BsaJI CCNNGG 5 cut(s) 145, 261, 294, 657, 1099
BsaXI ACNNNNNCTCC 4 cut(s) 250, 280, 930, 960
Bsc4I CCNNNNNNNGG 2 cut(s) 658, 1105
Bse1I ACTGG 2 cut(s) 1143, 1187
Bse21I CCTNAGG 2 cut(s) 566, 876
BseBI CCWGG 2 cut(s) 263, 296
BseCI ATCGAT 1 cut(s) 331
BseDI CCNNGG 5 cut(s) 145, 261, 294, 657, 1099
BseGI GGATG 6 cut(s) 86, 216, 577, 652, 1183, 1206
BseLI CCNNNNNNNGG 2 cut(s) 658, 1105
BseMII CTCAG 3 cut(s) 146, 580, 890
BseNI ACTGG 2 cut(s) 1143, 1187
BsgI GTGCAG 1 cut(s) 1171
BshFI GGCC 2 cut(s) 299, 1142
BshVI ATCGAT 1 cut(s) 331
BsiHKCI CYCGRG 1 cut(s) 656
BsiSI CCGG 1 cut(s) 1179
BslFI GGGAC 3 cut(s) 116, 617, 857
BslI CCNNNNNNNGG 2 cut(s) 658, 1105
BsmAI GTCTC 2 cut(s) 811, 842
BsmFI GGGAC 3 cut(s) 116, 617, 857
BsmI GAATGC 1 cut(s) 150
BsnI GGCC 2 cut(s) 299, 1142
BsoBI CYCGRG 1 cut(s) 656
Bsp119I TTCGAA 1 cut(s) 78
Bsp143I GATC 3 cut(s) 271, 340, 377
Bsp19I CCATGG 1 cut(s) 145
BspACI CCGC 2 cut(s) 1002, 1071
BspANI GGCC 2 cut(s) 299, 1142
BspCNI CTCAG 3 cut(s) 147, 579, 889
BspDI ATCGAT 1 cut(s) 331
BspHI TCATGA 1 cut(s) 795
BspLI GGNNCC 4 cut(s) 259, 267, 882, 1176
BspMAI CTGCAG 1 cut(s) 871
BspPI GGATC 2 cut(s) 335, 385
BspT104I TTCGAA 1 cut(s) 78
BspTI CTTAAG 1 cut(s) 686
BsrI ACTGG 2 cut(s) 1143, 1187
BssECI CCNNGG 5 cut(s) 145, 261, 294, 657, 1099
BssMI GATC 3 cut(s) 271, 340, 377
BssSI CACGAG 1 cut(s) 711
BssT1I CCWWGG 2 cut(s) 145, 1099
Bst2BI CACGAG 1 cut(s) 711
Bst2UI CCWGG 2 cut(s) 263, 296
Bst4CI ACNGT 4 cut(s) 115, 510, 1037, 1243
Bst6I CTCTTC 1 cut(s) 711
BstAFI CTTAAG 1 cut(s) 686
BstAPI GCANNNNNTGC 2 cut(s) 10, 199
BstBI TTCGAA 1 cut(s) 78
BstC8I GCNNGC 2 cut(s) 73, 834
BstDEI CTNAG 3 cut(s) 155, 566, 876
BstDSI CCRYGG 1 cut(s) 145
BstEII GGTNACC 1 cut(s) 946
BstENI CCTNNNNNAGG 1 cut(s) 1103
BstF5I GGATG 6 cut(s) 86, 216, 577, 652, 1183, 1206
BstKTI GATC 3 cut(s) 274, 343, 380
BstMAI GTCTC 2 cut(s) 811, 842
BstMBI GATC 3 cut(s) 271, 340, 377
BstMWI GCNNNNNNNGC 4 cut(s) 10, 199, 1001, 1079
BstNI CCWGG 2 cut(s) 263, 296
BstNSI RCATGY 2 cut(s) 193, 836
BstPI GGTNACC 1 cut(s) 946
BstSCI CCNGG 3 cut(s) 261, 294, 1177
BstSFI CTRYAG 1 cut(s) 867
BstV2I GAAGAC 2 cut(s) 112, 540
BstX2I RGATCY 1 cut(s) 271
BstXI CCANNNNNNTGG 1 cut(s) 1023
BstYI RGATCY 1 cut(s) 271
Bsu15I ATCGAT 1 cut(s) 331
Bsu36I CCTNAGG 2 cut(s) 566, 876
BsuRI GGCC 2 cut(s) 299, 1142
BsuTUI ATCGAT 1 cut(s) 331
BtgI CCRYGG 1 cut(s) 145
BtgZI GCGATG 1 cut(s) 1038
BtsCI GGATG 6 cut(s) 86, 216, 577, 652, 1183, 1206
BtsI GCAGTG 1 cut(s) 306
BtsIMutI CAGTG 2 cut(s) 111, 306
Cac8I GCNNGC 2 cut(s) 73, 834
CciI TCATGA 1 cut(s) 795
Cfr13I GGNCC 3 cut(s) 751, 880, 1085
ClaI ATCGAT 1 cut(s) 331
CseI GACGC 1 cut(s) 1260
Csp6I GTAC 3 cut(s) 862, 982, 1136
CviQI GTAC 3 cut(s) 862, 982, 1136
DdeI CTNAG 3 cut(s) 155, 566, 876
DpnI GATC 3 cut(s) 273, 342, 379
DpnII GATC 3 cut(s) 271, 340, 377
DriI GACNNNNNGTC 1 cut(s) 855
EaeI YGGCCR 1 cut(s) 1140
Eam1104I CTCTTC 1 cut(s) 711
Eam1105I GACNNNNNGTC 1 cut(s) 855
EarI CTCTTC 1 cut(s) 711
Eco130I CCWWGG 2 cut(s) 145, 1099
Eco147I AGGCCT 1 cut(s) 299
Eco47I GGWCC 3 cut(s) 751, 880, 1085
Eco57I CTGAAG 2 cut(s) 91, 106
Eco81I CCTNAGG 2 cut(s) 566, 876
Eco88I CYCGRG 1 cut(s) 656
Eco91I GGTNACC 1 cut(s) 946
EcoNI CCTNNNNNAGG 1 cut(s) 1103
EcoO109I RGGNCCY 2 cut(s) 751, 880
EcoO65I GGTNACC 1 cut(s) 946
EcoRII CCWGG 2 cut(s) 261, 294
EcoT14I CCWWGG 2 cut(s) 145, 1099
EcoT22I ATGCAT 2 cut(s) 1054, 1267
ErhI CCWWGG 2 cut(s) 145, 1099
FalI AAGNNNNNCTT 8 cut(s) 350, 382, 375, 407, 738, 770, 1214, 1246
FaqI GGGAC 3 cut(s) 116, 617, 857
FauNDI CATATG 1 cut(s) 227
FblI GTMKAC 1 cut(s) 186
Fnu4HI GCNGC 1 cut(s) 1002
FokI GGATG 6 cut(s) 73, 223, 584, 639, 1170, 1213
Fsp4HI GCNGC 1 cut(s) 1002
FspBI CTAG 1 cut(s) 1100
GluI GCNGC 1 cut(s) 1002
GsuI CTGGAG 1 cut(s) 252
HaeIII GGCC 2 cut(s) 299, 1142
HapII CCGG 1 cut(s) 1179
HgaI GACGC 1 cut(s) 1260
HindIII AAGCTT 3 cut(s) 73, 384, 1235
HpaII CCGG 1 cut(s) 1179
HphI GGTGA 2 cut(s) 542, 1060
Hpy166II GTNNAC 2 cut(s) 187, 1068
Hpy188III TCNNGA 8 cut(s) 218, 269, 513, 602, 656, 695, 796, 878
Hpy8I GTNNAC 2 cut(s) 187, 1068
HpyAV CCTTC 2 cut(s) 310, 1099
HpyCH4III ACNGT 4 cut(s) 115, 510, 1037, 1243
HpyCH4IV ACGT 1 cut(s) 954
HpyF10VI GCNNNNNNNGC 4 cut(s) 10, 199, 1001, 1079
HpyF3I CTNAG 3 cut(s) 155, 566, 876
HpySE526I ACGT 1 cut(s) 954
Kzo9I GATC 3 cut(s) 271, 340, 377
LmnI GCTCC 3 cut(s) 147, 271, 682
LweI GCATC 5 cut(s) 95, 173, 988, 1061, 1252
MaeI CTAG 1 cut(s) 1100
MaeII ACGT 1 cut(s) 954
MaeIII GTNAC 3 cut(s) 322, 946, 1037
MalI GATC 3 cut(s) 273, 342, 379
MboI GATC 3 cut(s) 271, 340, 377
MboII GAAGA 8 cut(s) 19, 112, 545, 710, 713, 728, 911, 1141
MfeI CAATTG 1 cut(s) 985
MflI RGATCY 1 cut(s) 271
MlsI TGGCCA 1 cut(s) 1142
MluNI TGGCCA 1 cut(s) 1142
MlyI GAGTC 7 cut(s) 166, 208, 215, 685, 786, 813, 1103
Mox20I TGGCCA 1 cut(s) 1142
Mph1103I ATGCAT 2 cut(s) 1054, 1267
MscI TGGCCA 1 cut(s) 1142
MseI TTAA 6 cut(s) 368, 551, 687, 1011, 1046, 1062
MslI CAYNNNNRTG 1 cut(s) 644
Msp20I TGGCCA 1 cut(s) 1142
MspCI CTTAAG 1 cut(s) 686
MspI CCGG 1 cut(s) 1179
MspR9I CCNGG 3 cut(s) 263, 296, 1179
MunI CAATTG 1 cut(s) 985
Mva1269I GAATGC 1 cut(s) 150
MvaI CCWGG 2 cut(s) 263, 296
MwoI GCNNNNNNNGC 4 cut(s) 10, 199, 1001, 1079
NciI CCSGG 1 cut(s) 1179
NcoI CCATGG 1 cut(s) 145
NdeI CATATG 1 cut(s) 227
NdeII GATC 3 cut(s) 271, 340, 377
NlaIV GGNNCC 4 cut(s) 259, 267, 882, 1176
NmeAIII GCCGAG 1 cut(s) 691
NmuCI GTSAC 1 cut(s) 322
NsiI ATGCAT 2 cut(s) 1054, 1267
NspI RCATGY 2 cut(s) 193, 836
NspV TTCGAA 1 cut(s) 78
PaeI GCATGC 1 cut(s) 836
PagI TCATGA 1 cut(s) 795
PceI AGGCCT 1 cut(s) 299
PctI GAATGC 1 cut(s) 150
PfeI GAWTC 6 cut(s) 24, 333, 516, 725, 758, 806
PkrI GCNGC 1 cut(s) 1003
PleI GAGTC 7 cut(s) 165, 208, 215, 685, 786, 813, 1103
PpsI GAGTC 7 cut(s) 165, 208, 215, 685, 786, 813, 1103
PpuMI RGGWCCY 2 cut(s) 751, 880
Psp5II RGGWCCY 2 cut(s) 751, 880
Psp6I CCWGG 2 cut(s) 261, 294
PspEI GGTNACC 1 cut(s) 946
PspGI CCWGG 2 cut(s) 261, 294
PspN4I GGNNCC 4 cut(s) 259, 267, 882, 1176
PspPI GGNCC 3 cut(s) 751, 880, 1085
PspPPI RGGWCCY 2 cut(s) 751, 880
PsrI GAACNNNNNNTAC 2 cut(s) 939, 971
PstI CTGCAG 1 cut(s) 871
PsuI RGATCY 1 cut(s) 271
RsaI GTAC 3 cut(s) 863, 983, 1137
RsaNI GTAC 3 cut(s) 862, 982, 1136
RseI CAYNNNNRTG 1 cut(s) 644
SaqAI TTAA 6 cut(s) 368, 551, 687, 1011, 1046, 1062
SatI GCNGC 1 cut(s) 1002
Sau3AI GATC 3 cut(s) 271, 340, 377
Sau96I GGNCC 3 cut(s) 751, 880, 1085
SchI GAGTC 7 cut(s) 166, 208, 215, 685, 786, 813, 1103
ScrFI CCNGG 3 cut(s) 263, 296, 1179
SfaNI GCATC 5 cut(s) 95, 173, 988, 1061, 1252
SfcI CTRYAG 1 cut(s) 867
SfuI TTCGAA 1 cut(s) 78
SinI GGWCC 3 cut(s) 751, 880, 1085
SmiMI CAYNNNNRTG 1 cut(s) 644
SmlI CTYRAG 1 cut(s) 686
SmoI CTYRAG 1 cut(s) 686
SphI GCATGC 1 cut(s) 836
SseBI AGGCCT 1 cut(s) 299
SsiI CCGC 2 cut(s) 1002, 1071
SspMI CTAG 1 cut(s) 1100
StuI AGGCCT 1 cut(s) 299
StyD4I CCNGG 3 cut(s) 261, 294, 1177
StyI CCWWGG 2 cut(s) 145, 1099
TaaI ACNGT 4 cut(s) 115, 510, 1037, 1243
TaiI ACGT 1 cut(s) 957
TaqI TCGA 5 cut(s) 78, 303, 331, 522, 965
TatI WGTACW 1 cut(s) 981
TauI GCSGC 1 cut(s) 1004
TfiI GAWTC 6 cut(s) 24, 333, 516, 725, 758, 806
Tru1I TTAA 6 cut(s) 368, 551, 687, 1011, 1046, 1062
Tru9I TTAA 6 cut(s) 368, 551, 687, 1011, 1046, 1062
TscAI CASTG 2 cut(s) 118, 313
TseFI GTSAC 1 cut(s) 322
Tsp45I GTSAC 1 cut(s) 322
TspGWI ACGGA 2 cut(s) 695, 845
TspRI CASTG 2 cut(s) 118, 313
Vha464I CTTAAG 1 cut(s) 686
VpaK11BI GGWCC 3 cut(s) 751, 880, 1085
XagI CCTNNNNNAGG 1 cut(s) 1103
XapI RAATTY 3 cut(s) 80, 94, 362
XceI RCATGY 2 cut(s) 193, 836
XcmI CCANNNNNNNNNTGG 1 cut(s) 232
XmaJI CCTAGG 1 cut(s) 1099
XmiI GTMKAC 1 cut(s) 186
XspI CTAG 1 cut(s) 1100
Zsp2I ATGCAT 2 cut(s) 1054, 1267
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.