Rh5AG396900

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0002264
Physical Location & Seq
Reverse (-)
68760455 .. 68761137
683 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG396900.1

Sequence Viewer

Length: 477 bp
ATGAGTATTAGCCCAACTGGACAGCAGGACCTGGAACAACTGATGAAACTCTGTGATCCTCCAACAAAAGAATTAGAGGAAATCCTAATCGAGTCCACTAACGCTGCTGAATCACAAACAGGTTGCAAGGTAGAAGAACCAAAGGCTTCCGGTTACACGTATCATTGTAGGATTTGCGATGATAATTGTGATCACAGCACTGAGACGTGCCCTGACAGATCTGGAAAATTTATAACAGTTTGCAGGGTTTGTGACTGTCTCCCATGTAAAAATGAGGCTGACCACAAGGAAGACCATTATGAAGATTTATTGTTTTGTCATCTTTGTAATACGATTGGTGAGCACTTGACTGAAATGTGCCCTGACATGGATTCCGACATGGGTGACGAGGACTGGGGTTCTGACATGGATGACGAGAGCAGGGATGAGAAGGGTGATTTTCCGGAGAATACACACAGCGCCGCCAATGCTGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

158

Amino Acids

17.68

Weight (kDa)

4.24

Isoelectric Point (pI)

54.81

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 233
AccIII TCCGGA 1 cut(s) 442
AciI CCGC 1 cut(s) 462
AclWI GGATC 1 cut(s) 50
AcsI RAATTY 1 cut(s) 227
AfiI CCNNNNNNNGG 1 cut(s) 367
AflIII ACRYGT 1 cut(s) 156
AjiI CACGTC 1 cut(s) 207
AjnI CCWGG 1 cut(s) 30
Alw21I GWGCWC 1 cut(s) 345
Alw26I GTCTC 2 cut(s) 197, 263
AlwI GGATC 1 cut(s) 50
AlwNI CAGNNNCTG 1 cut(s) 31
Aor13HI TCCGGA 1 cut(s) 442
ApeKI GCWGC 2 cut(s) 104, 470
ApoI RAATTY 1 cut(s) 227
AspLEI GCGC 1 cut(s) 461
AspS9I GGNCC 1 cut(s) 28
AsuHPI GGTGA 3 cut(s) 350, 395, 446
AvaII GGWCC 1 cut(s) 28
BaeGI GKGCMC 2 cut(s) 212, 362
BbsI GAAGAC 1 cut(s) 297
Bbv12I GWGCWC 1 cut(s) 345
BbvI GCAGC 2 cut(s) 91, 457
BciT130I CCWGG 1 cut(s) 32
BclI TGATCA 1 cut(s) 190
BcoDI GTCTC 2 cut(s) 197, 263
BfoI RGCGCY 1 cut(s) 462
BglII AGATCT 1 cut(s) 218
BisI GCNGC 3 cut(s) 105, 462, 471
BlsI GCNGC 3 cut(s) 106, 463, 472
Bme1390I CCNGG 1 cut(s) 32
Bme18I GGWCC 1 cut(s) 28
BmgBI CACGTC 1 cut(s) 207
BmgT120I GGNCC 1 cut(s) 28
BmrFI CCNGG 1 cut(s) 32
BmrI ACTGGG 1 cut(s) 403
BmuI ACTGGG 1 cut(s) 403
BpiI GAAGAC 1 cut(s) 297
BsaAI YACGTR 1 cut(s) 159
BsaWI WCCGGW 2 cut(s) 149, 442
Bsc4I CCNNNNNNNGG 1 cut(s) 367
Bse1I ACTGG 2 cut(s) 22, 398
BseAI TCCGGA 1 cut(s) 442
BseBI CCWGG 1 cut(s) 32
BseGI GGATG 2 cut(s) 415, 430
BseLI CCNNNNNNNGG 1 cut(s) 367
BseMII CTCAG 1 cut(s) 192
BseNI ACTGG 2 cut(s) 22, 398
BseSI GKGCMC 2 cut(s) 212, 362
BseXI GCAGC 2 cut(s) 91, 457
BsiHKAI GWGCWC 1 cut(s) 345
BsiSI CCGG 2 cut(s) 150, 443
BslI CCNNNNNNNGG 1 cut(s) 367
BsmAI GTCTC 2 cut(s) 197, 263
BsmBI CGTCTC 1 cut(s) 197
Bsp1286I GDGCHC 3 cut(s) 212, 345, 362
Bsp13I TCCGGA 1 cut(s) 442
Bsp143I GATC 3 cut(s) 55, 190, 218
BspACI CCGC 1 cut(s) 462
BspCNI CTCAG 1 cut(s) 193
BspEI TCCGGA 1 cut(s) 442
BspPI GGATC 1 cut(s) 50
BsrI ACTGG 2 cut(s) 22, 398
BssMI GATC 3 cut(s) 55, 190, 218
Bst2UI CCWGG 1 cut(s) 32
Bst4CI ACNGT 2 cut(s) 238, 257
BstBAI YACGTR 1 cut(s) 159
BstDEI CTNAG 1 cut(s) 201
BstF5I GGATG 2 cut(s) 415, 430
BstH2I RGCGCY 1 cut(s) 462
BstHHI GCGC 1 cut(s) 461
BstKTI GATC 3 cut(s) 58, 193, 221
BstMAI GTCTC 2 cut(s) 197, 263
BstMBI GATC 3 cut(s) 55, 190, 218
BstMWI GCNNNNNNNGC 2 cut(s) 467, 470
BstNI CCWGG 1 cut(s) 32
BstSCI CCNGG 1 cut(s) 30
BstSLI GKGCMC 2 cut(s) 212, 362
BstV1I GCAGC 2 cut(s) 91, 457
BstV2I GAAGAC 1 cut(s) 297
BstX2I RGATCY 1 cut(s) 218
BstYI RGATCY 1 cut(s) 218
BtgZI GCGATG 1 cut(s) 192
BtrI CACGTC 1 cut(s) 207
BtsCI GGATG 2 cut(s) 415, 430
BtsIMutI CAGTG 1 cut(s) 198
CaiI CAGNNNCTG 1 cut(s) 31
CfoI GCGC 1 cut(s) 461
Cfr13I GGNCC 1 cut(s) 28
CviAII CATG 4 cut(s) 264, 367, 379, 406
CviJI RGCY 3 cut(s) 12, 146, 278
CviKI_1 RGCY 3 cut(s) 12, 146, 278
DdeI CTNAG 1 cut(s) 201
DpnI GATC 3 cut(s) 57, 192, 220
DpnII GATC 3 cut(s) 55, 190, 218
Eco47I GGWCC 1 cut(s) 28
EcoO109I RGGNCCY 1 cut(s) 28
EcoRII CCWGG 1 cut(s) 30
Esp3I CGTCTC 1 cut(s) 197
FaeI CATG 4 cut(s) 267, 370, 382, 409
FaiI YATR 7 cut(s) 233, 265, 300, 368, 380, 407, 475
FatI CATG 4 cut(s) 263, 366, 378, 405
FbaI TGATCA 1 cut(s) 190
Fnu4HI GCNGC 3 cut(s) 105, 462, 471
FokI GGATG 2 cut(s) 422, 437
Fsp4HI GCNGC 3 cut(s) 105, 462, 471
GlaI GCGC 1 cut(s) 460
GluI GCNGC 3 cut(s) 105, 462, 471
HaeII RGCGCY 1 cut(s) 462
HapII CCGG 2 cut(s) 150, 443
HhaI GCGC 1 cut(s) 461
Hin1II CATG 4 cut(s) 267, 370, 382, 409
Hin6I GCGC 1 cut(s) 459
HinP1I GCGC 1 cut(s) 459
HinfI GANTC 3 cut(s) 92, 110, 371
HpaII CCGG 2 cut(s) 150, 443
HphI GGTGA 3 cut(s) 350, 395, 446
Hpy166II GTNNAC 1 cut(s) 96
Hpy188I TCNGA 2 cut(s) 376, 403
Hpy188III TCNNGA 2 cut(s) 222, 443
Hpy8I GTNNAC 1 cut(s) 96
HpyAV CCTTC 1 cut(s) 424
HpyCH4III ACNGT 2 cut(s) 238, 257
HpyCH4IV ACGT 2 cut(s) 158, 206
HpyCH4V TGCA 3 cut(s) 126, 243, 473
HpyF10VI GCNNNNNNNGC 2 cut(s) 467, 470
HpyF3I CTNAG 1 cut(s) 201
HpySE526I ACGT 2 cut(s) 158, 206
Hsp92II CATG 4 cut(s) 267, 370, 382, 409
HspAI GCGC 1 cut(s) 459
Kpn2I TCCGGA 1 cut(s) 442
Ksp22I TGATCA 1 cut(s) 190
Kzo9I GATC 3 cut(s) 55, 190, 218
Lsp1109I GCAGC 2 cut(s) 91, 457
MaeII ACGT 2 cut(s) 158, 206
MaeIII GTNAC 3 cut(s) 152, 251, 383
MalI GATC 3 cut(s) 57, 192, 220
MboI GATC 3 cut(s) 55, 190, 218
MboII GAAGA 3 cut(s) 146, 302, 314
MflI RGATCY 1 cut(s) 218
MhlI GDGCHC 3 cut(s) 212, 345, 362
MluCI AATT 3 cut(s) 71, 184, 227
MlyI GAGTC 1 cut(s) 101
MmeI TCCRAC 2 cut(s) 86, 399
MnlI CCTC 4 cut(s) 69, 70, 268, 382
MroI TCCGGA 1 cut(s) 442
MspI CCGG 2 cut(s) 150, 443
MspR9I CCNGG 1 cut(s) 32
MvaI CCWGG 1 cut(s) 32
MwoI GCNNNNNNNGC 2 cut(s) 467, 470
NdeII GATC 3 cut(s) 55, 190, 218
NlaIII CATG 4 cut(s) 267, 370, 382, 409
NmuCI GTSAC 2 cut(s) 251, 383
PfeI GAWTC 2 cut(s) 110, 371
PkrI GCNGC 3 cut(s) 106, 463, 472
PleI GAGTC 1 cut(s) 100
PpsI GAGTC 1 cut(s) 100
Ppu21I YACGTR 1 cut(s) 159
PpuMI RGGWCCY 1 cut(s) 28
PsiI TTATAA 1 cut(s) 233
Psp5II RGGWCCY 1 cut(s) 28
Psp6I CCWGG 1 cut(s) 30
PspGI CCWGG 1 cut(s) 30
PspPI GGNCC 1 cut(s) 28
PspPPI RGGWCCY 1 cut(s) 28
PstNI CAGNNNCTG 1 cut(s) 31
PsuI RGATCY 1 cut(s) 218
SatI GCNGC 3 cut(s) 105, 462, 471
Sau3AI GATC 3 cut(s) 55, 190, 218
Sau96I GGNCC 1 cut(s) 28
SchI GAGTC 1 cut(s) 101
ScrFI CCNGG 1 cut(s) 32
SduI GDGCHC 3 cut(s) 212, 345, 362
SetI ASST 5 cut(s) 33, 124, 132, 161, 209
SinI GGWCC 1 cut(s) 28
Sse9I AATT 3 cut(s) 71, 184, 227
SsiI CCGC 1 cut(s) 462
StyD4I CCNGG 1 cut(s) 30
TaaI ACNGT 2 cut(s) 238, 257
TaiI ACGT 2 cut(s) 161, 209
TaqI TCGA 1 cut(s) 90
TasI AATT 3 cut(s) 71, 184, 227
TauI GCSGC 1 cut(s) 464
TfiI GAWTC 2 cut(s) 110, 371
TscAI CASTG 1 cut(s) 205
TseFI GTSAC 2 cut(s) 251, 383
TseI GCWGC 2 cut(s) 104, 470
Tsp45I GTSAC 2 cut(s) 251, 383
TspDTI ATGAA 2 cut(s) 59, 315
TspRI CASTG 1 cut(s) 205
VpaK11BI GGWCC 1 cut(s) 28
XapI RAATTY 1 cut(s) 227
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.