RH5BG028300

No description available

Design Primers CRISPR Design

Basic Information

Type: Sequence Only
Biological Identity
rosa_samantha
Unknown
OG0002264
Physical Location & Seq
Reverse (-)
0 .. 0
1 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG028300.1

Sequence Viewer

Length: 678 bp
ATGGATCTCACTATATCTACGTTCTCTCAACGCCGAAACGAAAAACCTAAACCCAACTTGTCCAGTCTCGGTCGTAATCGAAGACCTTCTCAGAGAAAGAGACTGCTTTACATTCAAATCGAACCAGCTATGAGTATCTGGATGATCTGCAACCAGGTGTACCATAAGGCTCCTGGTATGCTTTTTGTTTCAGGGTTCCTGAAGAAAATTATCCGCCACTTTCTTGGACTGCTCCGAGTGTTTCGAGGAGAACAAGATACAGCCGCCAGCGCAATTGGCCAGCACCAGAAGCAGGCTCCGCCGGCGAAGCTTCATGATCCTCGACCGGGAGAACTCGAGGAGATTCTCATCGCTACTGGTAGCAGGGTAGAAGAACGAGACCCTTCTGATGACAGGCATTGTAGGATTTGCGATACAAGTGGTGATCATAGTACTGAAACGTGCCCCGAAAGATCCAAGGCATATGTAGTGCTTTGTGGGATTTGTGATGATGGCCCATGCGAAAATGAGGCTGACCACAAGGAAGAACATTACGAAGAACTCCTTTTTTGTTATATTTGTAACAAGGTTGGTGAGCACTGGACTGAAAATTGCCCTGACAACGATTGTGACTTTGATCCTCCCAACGGCTGGGACATTACAGAGAATAAACGGATAGATTTGTTCCCTCCGTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

225

Amino Acids

25.81

Weight (kDa)

5.73

Isoelectric Point (pI)

57.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 630
AciI CCGC 3 cut(s) 214, 264, 299
AclWI GGATC 4 cut(s) 12, 311, 447, 611
AcoI YGGCCR 1 cut(s) 277
AcuI CTGAAG 1 cut(s) 221
AfaI GTAC 2 cut(s) 161, 433
AfiI CCNNNNNNNGG 4 cut(s) 292, 326, 626, 630
AgsI TTSAA 1 cut(s) 116
AjnI CCWGG 2 cut(s) 153, 172
AluBI AGCT 2 cut(s) 128, 310
AluI AGCT 2 cut(s) 128, 310
Alw21I GWGCWC 1 cut(s) 579
Alw26I GTCTC 3 cut(s) 71, 94, 372
AlwI GGATC 4 cut(s) 12, 311, 447, 611
Ama87I CYCGRG 1 cut(s) 335
AoxI GGCC 2 cut(s) 277, 493
Asp700I GAANNNNTTC 1 cut(s) 85
AspLEI GCGC 1 cut(s) 272
AspS9I GGNCC 1 cut(s) 494
AsuC2I CCSGG 1 cut(s) 327
AsuHPI GGTGA 2 cut(s) 434, 584
AvaI CYCGRG 1 cut(s) 335
BaeGI GKGCMC 1 cut(s) 446
BalI TGGCCA 1 cut(s) 279
BbsI GAAGAC 1 cut(s) 88
Bbv12I GWGCWC 1 cut(s) 579
BccI CCATC 1 cut(s) 485
BceAI ACGGC 1 cut(s) 643
BciT130I CCWGG 2 cut(s) 155, 174
BclI TGATCA 1 cut(s) 424
BcnI CCSGG 1 cut(s) 327
BcoDI GTCTC 3 cut(s) 71, 94, 372
BisI GCNGC 1 cut(s) 264
BlsI GCNGC 1 cut(s) 265
BmcAI AGTACT 1 cut(s) 433
Bme1390I CCNGG 3 cut(s) 155, 174, 327
BmeT110I CYCGRG 1 cut(s) 335
BmgT120I GGNCC 1 cut(s) 494
BmiI GGNNCC 3 cut(s) 171, 197, 297
BmrFI CCNGG 3 cut(s) 155, 174, 327
BpiI GAAGAC 1 cut(s) 88
BpuMI CCSGG 1 cut(s) 327
BsaBI GATNNNNATC 1 cut(s) 347
BsaI GGTCTC 1 cut(s) 372
BsaJI CCNNGG 1 cut(s) 456
Bsc4I CCNNNNNNNGG 4 cut(s) 292, 326, 626, 630
Bse118I RCCGGY 1 cut(s) 301
Bse1I ACTGG 3 cut(s) 63, 361, 584
Bse8I GATNNNNATC 1 cut(s) 347
BseBI CCWGG 2 cut(s) 155, 174
BseDI CCNNGG 1 cut(s) 456
BseGI GGATG 1 cut(s) 147
BseJI GATNNNNATC 1 cut(s) 347
BseLI CCNNNNNNNGG 4 cut(s) 292, 326, 626, 630
BseMII CTCAG 1 cut(s) 104
BseNI ACTGG 3 cut(s) 63, 361, 584
BseRI GAGGAG 2 cut(s) 261, 353
BseSI GKGCMC 1 cut(s) 446
BseYI CCCAGC 1 cut(s) 630
Bsh1285I CGRYCG 2 cut(s) 73, 326
BshFI GGCC 2 cut(s) 279, 495
BsiEI CGRYCG 2 cut(s) 73, 326
BsiHKAI GWGCWC 1 cut(s) 579
BsiHKCI CYCGRG 1 cut(s) 335
BsiSI CCGG 2 cut(s) 302, 326
BslFI GGGAC 1 cut(s) 647
BslI CCNNNNNNNGG 4 cut(s) 292, 326, 626, 630
BsmAI GTCTC 3 cut(s) 71, 94, 372
BsmFI GGGAC 1 cut(s) 647
BsnI GGCC 2 cut(s) 279, 495
Bso31I GGTCTC 1 cut(s) 372
BsoBI CYCGRG 1 cut(s) 335
Bsp1286I GDGCHC 2 cut(s) 446, 579
Bsp143I GATC 6 cut(s) 4, 144, 316, 424, 452, 616
BspACI CCGC 3 cut(s) 214, 264, 299
BspANI GGCC 2 cut(s) 279, 495
BspCNI CTCAG 1 cut(s) 103
BspHI TCATGA 1 cut(s) 313
BspLI GGNNCC 3 cut(s) 171, 197, 297
BspPI GGATC 4 cut(s) 12, 311, 447, 611
BspTNI GGTCTC 1 cut(s) 372
BsrFI RCCGGY 1 cut(s) 301
BsrI ACTGG 3 cut(s) 63, 361, 584
BssAI RCCGGY 1 cut(s) 301
BssECI CCNNGG 1 cut(s) 456
BssMI GATC 6 cut(s) 4, 144, 316, 424, 452, 616
BssT1I CCWWGG 1 cut(s) 456
Bst2UI CCWGG 2 cut(s) 155, 174
BstC8I GCNNGC 4 cut(s) 268, 281, 294, 303
BstDEI CTNAG 1 cut(s) 90
BstF5I GGATG 1 cut(s) 147
BstHHI GCGC 1 cut(s) 272
BstKTI GATC 6 cut(s) 7, 147, 319, 427, 455, 619
BstMAI GTCTC 3 cut(s) 71, 94, 372
BstMBI GATC 6 cut(s) 4, 144, 316, 424, 452, 616
BstMCI CGRYCG 2 cut(s) 73, 326
BstMWI GCNNNNNNNGC 6 cut(s) 269, 276, 289, 298, 302, 307
BstNI CCWGG 2 cut(s) 155, 174
BstSCI CCNGG 3 cut(s) 153, 172, 325
BstSLI GKGCMC 1 cut(s) 446
BstV2I GAAGAC 1 cut(s) 88
BstX2I RGATCY 2 cut(s) 4, 452
BstXI CCANNNNNNTGG 1 cut(s) 224
BstYI RGATCY 2 cut(s) 4, 452
BsuRI GGCC 2 cut(s) 279, 495
BtgZI GCGATG 1 cut(s) 334
BtsCI GGATG 1 cut(s) 147
BtsIMutI CAGTG 1 cut(s) 577
Cac8I GCNNGC 4 cut(s) 268, 281, 294, 303
CciI TCATGA 1 cut(s) 313
CfoI GCGC 1 cut(s) 272
Cfr10I RCCGGY 1 cut(s) 301
Cfr13I GGNCC 1 cut(s) 494
CsiI ACCWGGT 1 cut(s) 153
Csp6I GTAC 2 cut(s) 160, 432
CviAII CATG 2 cut(s) 314, 498
CviJI RGCY 9 cut(s) 128, 170, 263, 279, 296, 310, 495, 512, 630
CviKI_1 RGCY 9 cut(s) 128, 170, 263, 279, 296, 310, 495, 512, 630
CviQI GTAC 2 cut(s) 160, 432
DdeI CTNAG 1 cut(s) 90
DpnI GATC 6 cut(s) 6, 146, 318, 426, 454, 618
DpnII GATC 6 cut(s) 4, 144, 316, 424, 452, 616
EaeI YGGCCR 1 cut(s) 277
EciI GGCGGA 2 cut(s) 203, 288
Eco130I CCWWGG 1 cut(s) 456
Eco31I GGTCTC 1 cut(s) 372
Eco57I CTGAAG 1 cut(s) 221
Eco88I CYCGRG 1 cut(s) 335
EcoRII CCWGG 2 cut(s) 153, 172
EcoT14I CCWWGG 1 cut(s) 456
ErhI CCWWGG 1 cut(s) 456
FaeI CATG 2 cut(s) 317, 501
FalI AAGNNNNNCTT 2 cut(s) 528, 560
FaqI GGGAC 1 cut(s) 647
FatI CATG 2 cut(s) 313, 497
FauNDI CATATG 1 cut(s) 463
FbaI TGATCA 1 cut(s) 424
Fnu4HI GCNGC 1 cut(s) 264
FokI GGATG 1 cut(s) 154
Fsp4HI GCNGC 1 cut(s) 264
GlaI GCGC 1 cut(s) 271
GluI GCNGC 1 cut(s) 264
GsaI CCCAGC 1 cut(s) 634
HaeIII GGCC 2 cut(s) 279, 495
HapII CCGG 2 cut(s) 302, 326
HhaI GCGC 1 cut(s) 272
Hin1II CATG 2 cut(s) 317, 501
Hin6I GCGC 1 cut(s) 270
HinP1I GCGC 1 cut(s) 270
HindIII AAGCTT 1 cut(s) 308
HinfI GANTC 1 cut(s) 343
HpaII CCGG 2 cut(s) 302, 326
HphI GGTGA 2 cut(s) 434, 584
Hpy166II GTNNAC 1 cut(s) 160
Hpy188I TCNGA 3 cut(s) 93, 236, 388
Hpy188III TCNNGA 3 cut(s) 139, 199, 314
Hpy8I GTNNAC 1 cut(s) 160
HpyAV CCTTC 2 cut(s) 96, 393
HpyCH4IV ACGT 2 cut(s) 20, 440
HpyCH4V TGCA 1 cut(s) 150
HpyF10VI GCNNNNNNNGC 6 cut(s) 269, 276, 289, 298, 302, 307
HpyF3I CTNAG 1 cut(s) 90
HpySE526I ACGT 2 cut(s) 20, 440
Hsp92II CATG 2 cut(s) 317, 501
HspAI GCGC 1 cut(s) 270
KroI GCCGGC 1 cut(s) 301
KroNI GCCGGC 1 cut(s) 303
Ksp22I TGATCA 1 cut(s) 424
Kzo9I GATC 6 cut(s) 4, 144, 316, 424, 452, 616
LmnI GCTCC 3 cut(s) 175, 237, 301
MabI ACCWGGT 1 cut(s) 153
MaeII ACGT 2 cut(s) 20, 440
MaeIII GTNAC 2 cut(s) 560, 608
MalI GATC 6 cut(s) 6, 146, 318, 426, 454, 618
MboI GATC 6 cut(s) 4, 144, 316, 424, 452, 616
MboII GAAGA 5 cut(s) 93, 214, 383, 536, 548
MfeI CAATTG 1 cut(s) 273
MflI RGATCY 2 cut(s) 4, 452
MhlI GDGCHC 2 cut(s) 446, 579
MlsI TGGCCA 1 cut(s) 279
MluCI AATT 3 cut(s) 207, 273, 589
MluNI TGGCCA 1 cut(s) 279
MnlI CCTC 6 cut(s) 239, 330, 331, 502, 630, 678
Mox20I TGGCCA 1 cut(s) 279
MreI CGCCGGCG 1 cut(s) 301
MroNI GCCGGC 1 cut(s) 301
MroXI GAANNNNTTC 1 cut(s) 85
MscI TGGCCA 1 cut(s) 279
Msp20I TGGCCA 1 cut(s) 279
MspI CCGG 2 cut(s) 302, 326
MspR9I CCNGG 3 cut(s) 155, 174, 327
MunI CAATTG 1 cut(s) 273
MvaI CCWGG 2 cut(s) 155, 174
MwoI GCNNNNNNNGC 6 cut(s) 269, 276, 289, 298, 302, 307
NaeI GCCGGC 1 cut(s) 303
NciI CCSGG 1 cut(s) 327
NdeI CATATG 1 cut(s) 463
NdeII GATC 6 cut(s) 4, 144, 316, 424, 452, 616
NgoMIV GCCGGC 1 cut(s) 301
NlaIII CATG 2 cut(s) 317, 501
NlaIV GGNNCC 3 cut(s) 171, 197, 297
NmuCI GTSAC 1 cut(s) 608
PaeR7I CTCGAG 1 cut(s) 335
PagI TCATGA 1 cut(s) 313
PdiI GCCGGC 1 cut(s) 303
PdmI GAANNNNTTC 1 cut(s) 85
PfeI GAWTC 1 cut(s) 343
PflMI CCANNNNNTGG 1 cut(s) 630
PkrI GCNGC 1 cut(s) 265
Psp6I CCWGG 2 cut(s) 153, 172
PspFI CCCAGC 1 cut(s) 630
PspGI CCWGG 2 cut(s) 153, 172
PspN4I GGNNCC 3 cut(s) 171, 197, 297
PspPI GGNCC 1 cut(s) 494
PspXI VCTCGAGB 1 cut(s) 335
PsuI RGATCY 2 cut(s) 4, 452
RsaI GTAC 2 cut(s) 161, 433
RsaNI GTAC 2 cut(s) 160, 432
SatI GCNGC 1 cut(s) 264
Sau3AI GATC 6 cut(s) 4, 144, 316, 424, 452, 616
Sau96I GGNCC 1 cut(s) 494
ScaI AGTACT 1 cut(s) 433
ScrFI CCNGG 3 cut(s) 155, 174, 327
SduI GDGCHC 2 cut(s) 446, 579
SetI ASST 8 cut(s) 23, 49, 88, 130, 159, 312, 443, 570
SexAI ACCWGGT 1 cut(s) 153
Sfr274I CTCGAG 1 cut(s) 335
SgrAI CRCCGGYG 1 cut(s) 301
SlaI CTCGAG 1 cut(s) 335
SmlI CTYRAG 1 cut(s) 335
SmoI CTYRAG 1 cut(s) 335
Sse9I AATT 3 cut(s) 207, 273, 589
SsiI CCGC 3 cut(s) 214, 264, 299
StyD4I CCNGG 3 cut(s) 153, 172, 325
StyI CCWWGG 1 cut(s) 456
TaiI ACGT 2 cut(s) 23, 443
TaqI TCGA 5 cut(s) 79, 120, 244, 322, 336
TaqII GACCGA 1 cut(s) 59
TasI AATT 3 cut(s) 207, 273, 589
TatI WGTACW 1 cut(s) 431
TauI GCSGC 1 cut(s) 266
TfiI GAWTC 1 cut(s) 343
TscAI CASTG 1 cut(s) 584
TseFI GTSAC 1 cut(s) 608
Tsp45I GTSAC 1 cut(s) 608
TspDTI ATGAA 1 cut(s) 302
TspGWI ACGGA 2 cut(s) 660, 667
TspRI CASTG 1 cut(s) 584
Van91I CCANNNNNTGG 1 cut(s) 630
XcmI CCANNNNNNNNNTGG 1 cut(s) 170
XhoI CTCGAG 1 cut(s) 335
XmnI GAANNNNTTC 1 cut(s) 85
ZrmI AGTACT 1 cut(s) 433
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.