FvH4_3g20963

B3 DNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0001196
Physical Location & Seq
Reverse (-)
13998846 .. 14007491
8646 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g20963.t15

Sequence Viewer

Length: 1710 bp
ATGGCTTCCAAAGGTTCGTGCTTTTTCTGCAAGGCCGAGAGTGATGTGTTCGTTGATGGCTGGAAGCTCAAGGATGGGTCTTCGGCCCAGCTCTGCAGGAAATGCGGTTTTGCTTATGTGAATGGTATATTTTGTGAAACTTTTCACATGGGAGCGGAAGGCTGGAGAGACTGTGCTGTTTGTGGAAAGCCACTCCATTGTGGGTGCATTATGTCATGCCACAAGTATGCAGCACTGGATGTCGGAGGTGTAGGTTGCTCAAACTGCTTACTAGATGTTGATAAAGCTATTGCTTCCGGTCAAATGACCCAAATGCTGAAAGAATTGGTTGCCAAGTACAAGACGGATCCAACGGTTACGTTTGATCCTACTAGGAAAGTTGTCGAGTCCCTTTCTCCTGGATCTGTACAAGTGCCTTCTCCTCAGTTTGGAGCATCTAAAGGAGATACATCTTCTGAAACCAAAGTAGACAGTGAAACACCTCAAATGGATGCCTCCACAGGGAACCATTTACACACTGACAATTGGCTGAAGACGACAAGTGCTGAGCAACTCCCAAAAGTCTCTGGAAACTTAATCTCTTGTCATATATCTCTGTTTGAGAAGTCATCCACTGCAAGTGTAGCTAATTGCAGTACAGGATGTCTTGTGATACTGAAGAAATATGCTGAGAAACAGACTCCAAGGGACATTCAAACTGGTCCAACCCCAAAAGATACAAGTCTGAAAGCAGACCAGGAACAATCTCCGATGGATATGGAAACAAATTCGATGCCTATGGAAACAAATCAGAAGACAAATCAGAAAAGAAACCAGGAACAATCTCCTAGGGACATTGAAACAGGTCCAACGGCTGTAGGTAAAAGTCTGAAAGCAGACCAGAAACAGACTCCAAGGGACGTTCAAACAGGTCCAATGCCTGTAAATTCAAGTATGAAAACAGAAAAGACTCCAAGGAACATTCAAACAGGTCCAATGCCTGTAAATTCAAGTATGAAAACAGACAAGAAACAGACTCCAAGGGACGTTCAAACAGGTGCAATGCCTGTAAATTCAAGTATGAAAATAGATAAGAAACAGACTCCAAGGAACATTCAAACAGGTCCAATGCCTGTAAATTCAAGTATAAAAAGAGACAAGAAACAGACTCCAAGGGACATTCAAACTGGTCCAACCCCAAAAGATACAAGTCTGAAAGCAGACCAGGAACAATCTCCGATGGATATGGAAACAATTTCGATGCCTATGGAAACAAATCAGAAAAGAAACCAGGAACAATCTCCTAGGGACATTGAAACAGGTCCAACGGCTGTAGGTAAAAGTCTGAAAGCAGACCAGAAACAGATTCCAAGGGACGTTCAAACAGGTCCAATGCCTGTAAATTCAAGTATGAAAACAGACAAGGAACAGACTAGGGACATTCAGACAGATTCAATGCCTGTAAATTCAAGTTTGAAAACAGGCAAGAAACAATCTCCAAGGAATTTGAAGACAAGTCCAATACCGGTACAAACAAGTCAGAAATCAGAACAGGGACAATCTCCAAGGGATATGGAAACAGGTCCAACACCTGTAGGAACAAGTGTGAAAACAGATCAGGAAGAATCTCAAAGGGAAATGGAAACAGGTCCATCACCTGATGAAAGAACTCTCAAAACAGACCAGGAACAGTCTACAAAGGATATGGAAACAAGTCTGAAAACAGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

570

Amino Acids

62.19

Weight (kDa)

8.22

Isoelectric Point (pI)

47.0

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 155
AccI GTMKAC 2 cut(s) 468, 1673
AciI CCGC 2 cut(s) 105, 155
AclWI GGATC 4 cut(s) 341, 354, 359, 409
AcsI RAATTY 8 cut(s) 766, 925, 985, 1051, 1117, 1381, 1444, 1483
AcuI CTGAAG 2 cut(s) 551, 677
AfaI GTAC 4 cut(s) 338, 408, 637, 1509
AfiI CCNNNNNNNGG 2 cut(s) 428, 501
AgeI ACCGGT 1 cut(s) 1504
AjnI CCWGG 6 cut(s) 397, 735, 813, 1203, 1269, 1662
AluBI AGCT 4 cut(s) 67, 91, 287, 626
AluI AGCT 4 cut(s) 67, 91, 287, 626
Alw26I GTCTC 3 cut(s) 162, 568, 1128
AlwI GGATC 4 cut(s) 341, 354, 359, 409
AoxI GGCC 2 cut(s) 33, 84
ApeKI GCWGC 1 cut(s) 230
ApoI RAATTY 8 cut(s) 766, 925, 985, 1051, 1117, 1381, 1444, 1483
AsiGI ACCGGT 1 cut(s) 1504
Asp700I GAANNNNTTC 1 cut(s) 141
AspA2I CCTAGG 2 cut(s) 827, 1283
AsuHPI GGTGA 1 cut(s) 1626
AvrII CCTAGG 2 cut(s) 827, 1283
BamHI GGATCC 1 cut(s) 346
BbsI GAAGAC 4 cut(s) 72, 539, 800, 1496
BbvI GCAGC 1 cut(s) 242
BccI CCATC 5 cut(s) 50, 68, 745, 1213, 1639
BceAI ACGGC 2 cut(s) 867, 1323
BciT130I CCWGG 6 cut(s) 399, 737, 815, 1205, 1271, 1664
BcoDI GTCTC 3 cut(s) 162, 568, 1128
BfaI CTAG 6 cut(s) 272, 372, 828, 1284, 1413, 1708
BfmI CTRYAG 4 cut(s) 94, 855, 1311, 1572
BisI GCNGC 1 cut(s) 231
BlnI CCTAGG 2 cut(s) 827, 1283
BlpI GCTNAGC 1 cut(s) 546
BlsI GCNGC 1 cut(s) 232
Bme1390I CCNGG 6 cut(s) 399, 737, 815, 1205, 1271, 1664
BmiI GGNNCC 2 cut(s) 348, 506
BmrFI CCNGG 6 cut(s) 399, 737, 815, 1205, 1271, 1664
BmsI GCATC 4 cut(s) 443, 481, 762, 1230
BpiI GAAGAC 4 cut(s) 72, 539, 800, 1496
BpmI CTGGAG 1 cut(s) 184
Bpu1102I GCTNAGC 1 cut(s) 546
BpuEI CTTGAG 1 cut(s) 53
BsaWI WCCGGW 2 cut(s) 296, 1504
BsaXI ACNNNNNCTCC 2 cut(s) 157, 187
Bsc4I CCNNNNNNNGG 2 cut(s) 428, 501
Bse118I RCCGGY 1 cut(s) 1504
Bse1I ACTGG 3 cut(s) 240, 703, 1171
Bse3DI GCAATG 1 cut(s) 1047
BseBI CCWGG 6 cut(s) 399, 737, 815, 1205, 1271, 1664
BseGI GGATG 5 cut(s) 79, 244, 496, 608, 647
BseLI CCNNNNNNNGG 2 cut(s) 428, 501
BseMI GCAATG 1 cut(s) 1047
BseMII CTCAG 3 cut(s) 437, 537, 660
BseNI ACTGG 3 cut(s) 240, 703, 1171
BseRI GAGGAG 1 cut(s) 411
BseXI GCAGC 1 cut(s) 242
BseYI CCCAGC 1 cut(s) 87
BshFI GGCC 2 cut(s) 35, 86
BshTI ACCGGT 1 cut(s) 1504
BsiSI CCGG 2 cut(s) 297, 1505
BslI CCNNNNNNNGG 2 cut(s) 428, 501
BsmAI GTCTC 3 cut(s) 162, 568, 1128
BsnI GGCC 2 cut(s) 35, 86
Bsp1407I TGTACA 1 cut(s) 406
Bsp143I GATC 4 cut(s) 346, 364, 401, 1594
Bsp1720I GCTNAGC 1 cut(s) 546
BspACI CCGC 2 cut(s) 105, 155
BspANI GGCC 2 cut(s) 35, 86
BspCNI CTCAG 3 cut(s) 436, 538, 661
BspLI GGNNCC 2 cut(s) 348, 506
BspMAI CTGCAG 1 cut(s) 98
BspPI GGATC 4 cut(s) 341, 354, 359, 409
BsrBI CCGCTC 1 cut(s) 155
BsrDI GCAATG 1 cut(s) 1047
BsrFI RCCGGY 1 cut(s) 1504
BsrGI TGTACA 1 cut(s) 406
BsrI ACTGG 3 cut(s) 240, 703, 1171
BssAI RCCGGY 1 cut(s) 1504
BssMI GATC 4 cut(s) 346, 364, 401, 1594
Bst2UI CCWGG 6 cut(s) 399, 737, 815, 1205, 1271, 1664
Bst4CI ACNGT 4 cut(s) 173, 355, 473, 1671
BstAPI GCANNNNNTGC 1 cut(s) 102
BstAUI TGTACA 1 cut(s) 406
BstDEI CTNAG 3 cut(s) 423, 546, 669
BstF5I GGATG 5 cut(s) 79, 244, 496, 608, 647
BstKTI GATC 4 cut(s) 349, 367, 404, 1597
BstMAI GTCTC 3 cut(s) 162, 568, 1128
BstMBI GATC 4 cut(s) 346, 364, 401, 1594
BstMWI GCNNNNNNNGC 4 cut(s) 27, 102, 264, 623
BstNI CCWGG 6 cut(s) 399, 737, 815, 1205, 1271, 1664
BstSCI CCNGG 6 cut(s) 397, 735, 813, 1203, 1269, 1662
BstSFI CTRYAG 4 cut(s) 94, 855, 1311, 1572
BstV1I GCAGC 1 cut(s) 242
BstV2I GAAGAC 4 cut(s) 72, 539, 800, 1496
BstX2I RGATCY 2 cut(s) 346, 401
BstYI RGATCY 2 cut(s) 346, 401
BsuRI GGCC 2 cut(s) 35, 86
BtsCI GGATG 5 cut(s) 79, 244, 496, 608, 647
BtsI GCAGTG 1 cut(s) 612
BtsIMutI CAGTG 4 cut(s) 233, 478, 516, 612
Cfr10I RCCGGY 1 cut(s) 1504
Csp6I GTAC 4 cut(s) 337, 407, 636, 1508
CspAI ACCGGT 1 cut(s) 1504
CviAII CATG 2 cut(s) 148, 216
CviQI GTAC 4 cut(s) 337, 407, 636, 1508
DdeI CTNAG 3 cut(s) 423, 546, 669
DpnI GATC 4 cut(s) 348, 366, 403, 1596
DpnII GATC 4 cut(s) 346, 364, 401, 1594
Eco57I CTGAAG 2 cut(s) 551, 677
EcoRII CCWGG 6 cut(s) 397, 735, 813, 1203, 1269, 1662
FaeI CATG 2 cut(s) 151, 219
FatI CATG 2 cut(s) 147, 215
FblI GTMKAC 2 cut(s) 468, 1673
Fnu4HI GCNGC 1 cut(s) 231
FokI GGATG 5 cut(s) 86, 251, 503, 595, 654
Fsp4HI GCNGC 1 cut(s) 231
FspBI CTAG 6 cut(s) 272, 372, 828, 1284, 1413, 1708
GluI GCNGC 1 cut(s) 231
GsaI CCCAGC 1 cut(s) 91
GsuI CTGGAG 1 cut(s) 184
HaeIII GGCC 2 cut(s) 35, 86
HapII CCGG 2 cut(s) 297, 1505
Hin1II CATG 2 cut(s) 151, 219
HpaII CCGG 2 cut(s) 297, 1505
HphI GGTGA 1 cut(s) 1626
Hpy166II GTNNAC 2 cut(s) 469, 1674
Hpy188III TCNNGA 2 cut(s) 567, 1598
Hpy8I GTNNAC 2 cut(s) 469, 1674
HpyAV CCTTC 2 cut(s) 152, 426
HpyCH4III ACNGT 4 cut(s) 173, 355, 473, 1671
HpyCH4IV ACGT 4 cut(s) 359, 900, 1026, 1356
HpyCH4V TGCA 7 cut(s) 30, 96, 207, 230, 617, 633, 1040
HpyF10VI GCNNNNNNNGC 4 cut(s) 27, 102, 264, 623
HpyF3I CTNAG 3 cut(s) 423, 546, 669
HpySE526I ACGT 4 cut(s) 359, 900, 1026, 1356
Hsp92II CATG 2 cut(s) 151, 219
Kzo9I GATC 4 cut(s) 346, 364, 401, 1594
LmnI GCTCC 2 cut(s) 152, 431
Lsp1109I GCAGC 1 cut(s) 242
LweI GCATC 4 cut(s) 443, 481, 762, 1230
MaeI CTAG 6 cut(s) 272, 372, 828, 1284, 1413, 1708
MaeII ACGT 4 cut(s) 359, 900, 1026, 1356
MaeIII GTNAC 1 cut(s) 355
MalI GATC 4 cut(s) 348, 366, 403, 1596
MbiI CCGCTC 1 cut(s) 155
MboI GATC 4 cut(s) 346, 364, 401, 1594
MboII GAAGA 7 cut(s) 72, 444, 544, 670, 805, 1501, 1613
MfeI CAATTG 1 cut(s) 523
MflI RGATCY 2 cut(s) 346, 401
MlyI GAGTC 7 cut(s) 395, 673, 883, 943, 1009, 1075, 1141
MmeI TCCRAC 7 cut(s) 223, 374, 728, 872, 1196, 1328, 1589
MnlI CCTC 4 cut(s) 239, 432, 492, 505
MroXI GAANNNNTTC 1 cut(s) 141
MseI TTAA 1 cut(s) 575
MslI CAYNNNNRTG 1 cut(s) 225
MspI CCGG 2 cut(s) 297, 1505
MspR9I CCNGG 6 cut(s) 399, 737, 815, 1205, 1271, 1664
MunI CAATTG 1 cut(s) 523
MvaI CCWGG 6 cut(s) 399, 737, 815, 1205, 1271, 1664
MwoI GCNNNNNNNGC 4 cut(s) 27, 102, 264, 623
NdeII GATC 4 cut(s) 346, 364, 401, 1594
NlaIII CATG 2 cut(s) 151, 219
NlaIV GGNNCC 2 cut(s) 348, 506
NmeAIII GCCGAG 1 cut(s) 61
PdmI GAANNNNTTC 1 cut(s) 141
PfeI GAWTC 3 cut(s) 1345, 1430, 1604
PfoI TCCNGGA 1 cut(s) 397
PinAI ACCGGT 1 cut(s) 1504
PkrI GCNGC 1 cut(s) 232
PleI GAGTC 7 cut(s) 394, 673, 883, 943, 1009, 1075, 1141
PpsI GAGTC 7 cut(s) 394, 673, 883, 943, 1009, 1075, 1141
Psp6I CCWGG 6 cut(s) 397, 735, 813, 1203, 1269, 1662
PspFI CCCAGC 1 cut(s) 87
PspGI CCWGG 6 cut(s) 397, 735, 813, 1203, 1269, 1662
PspN4I GGNNCC 2 cut(s) 348, 506
PstI CTGCAG 1 cut(s) 98
PsuI RGATCY 2 cut(s) 346, 401
RsaI GTAC 4 cut(s) 338, 408, 637, 1509
RsaNI GTAC 4 cut(s) 337, 407, 636, 1508
RseI CAYNNNNRTG 1 cut(s) 225
SaqAI TTAA 1 cut(s) 575
SatI GCNGC 1 cut(s) 231
Sau3AI GATC 4 cut(s) 346, 364, 401, 1594
SchI GAGTC 7 cut(s) 395, 673, 883, 943, 1009, 1075, 1141
ScrFI CCNGG 6 cut(s) 399, 737, 815, 1205, 1271, 1664
SfaNI GCATC 4 cut(s) 443, 481, 762, 1230
SfcI CTRYAG 4 cut(s) 94, 855, 1311, 1572
SmiMI CAYNNNNRTG 1 cut(s) 225
SmlI CTYRAG 1 cut(s) 68
SmoI CTYRAG 1 cut(s) 68
SsiI CCGC 2 cut(s) 105, 155
SspMI CTAG 6 cut(s) 272, 372, 828, 1284, 1413, 1708
StyD4I CCNGG 6 cut(s) 397, 735, 813, 1203, 1269, 1662
TaaI ACNGT 4 cut(s) 173, 355, 473, 1671
TaiI ACGT 4 cut(s) 362, 903, 1029, 1359
TaqI TCGA 3 cut(s) 384, 770, 1238
TatI WGTACW 3 cut(s) 336, 406, 635
TfiI GAWTC 3 cut(s) 1345, 1430, 1604
Tru1I TTAA 1 cut(s) 575
Tru9I TTAA 1 cut(s) 575
TscAI CASTG 4 cut(s) 240, 478, 523, 619
TseI GCWGC 1 cut(s) 230
TspDTI ATGAA 5 cut(s) 950, 1010, 1076, 1406, 1656
TspGWI ACGGA 1 cut(s) 359
TspRI CASTG 4 cut(s) 240, 478, 523, 619
XapI RAATTY 8 cut(s) 766, 925, 985, 1051, 1117, 1381, 1444, 1483
XmaJI CCTAGG 2 cut(s) 827, 1283
XmiI GTMKAC 2 cut(s) 468, 1673
XmnI GAANNNNTTC 1 cut(s) 141
XspI CTAG 6 cut(s) 272, 372, 828, 1284, 1413, 1708
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.