Rroxscaffold_1G00045010

B3 DNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0001196
Physical Location & Seq
Forward (+)
64075755 .. 64083140
7386 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00045010.1

Sequence Viewer

Length: 924 bp
ATGGCTGCCAAAGCTTCGTGCTTTTCCTGCAAGGCCGAGAGTGATCAGTTCGTTAATGGCTGGGAGCTCAAGGATGGGTCTTTCGCCCAGCTCTGCAAGAAATGCGGTTTTGCTTATGTGAATGGTATATTTTGTGAAACTTTTCACATGGAAGCGGAAGGCTGGAGAGACTGTGCTGTTTGTGGGAAGCCACTCCATTGTGGGTGCATTATGTCATGCAACAAGTATGCACCACTGGATTTCGGAGGTGTAGGTTGCTCAAAATGCTTACTAGAAGTTGATCAGTCTATTGCTTCAGGTCGAATGACCAGAATGCTGAAAGCAATAGTTGCCACGTATACGACAGATCCAACAGTTTCATCTGATCCTACTGAGAAAGTTGTTGAGTCCCTTTCTCCTGGATCTTCACAAGCGCCTGCTCCTCATTTTGGAGAAGAAGCCATTACGGATGCATCTAAAGGAGATACAGCTTGTGAAACCAAAGAACAATCTCCTAGGGACATTGAAACCGGCCCTACGCCTGTAGATACAAGTCTGAAAGTAGACCAGGAACAATCTCCAATGGATATGGAAACAAGTCCAATGCCTATAGTAACAAATCAGAAAACAAACCAGGAACACTCTCCAAGGGACATTGAAACAAGTCCAACACCTGTAGATACAAGTCTGAAAACAGACCAGGAACAAACTCCAAGGGACATTGAAACAGGTCCAACGCCTGTAGATACAAGTGTGAAAACAGACCAGGGACAATCTCCAAGGGATATGGAGACAAGTCCAACTTTTGTAGAAGCAAGTCGGAAATCAGACAAGGAACAATCTCAAAGGGATATGGAAACAGGTCCAACACCTGCAGAAACAACCCTTAAAACAGACCAGGAACAGTCTCCGAGGGGCATGGAAACAAGTCTGAAAACAGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

307

Amino Acids

33.31

Weight (kDa)

4.61

Isoelectric Point (pI)

48.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf_VAL1_N PF25813 47 - 91 1.6e-20 VAL1-like, N-terminal zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 859
Acc36I ACCTGC 1 cut(s) 859
AccI GTMKAC 2 cut(s) 338, 543
AciI CCGC 2 cut(s) 105, 155
AclWI GGATC 3 cut(s) 341, 359, 409
AcuI CTGAAG 1 cut(s) 279
AfiI CCNNNNNNNGG 1 cut(s) 428
AgsI TTSAA 3 cut(s) 506, 638, 704
AjnI CCWGG 6 cut(s) 397, 546, 612, 678, 744, 876
AluBI AGCT 4 cut(s) 14, 67, 91, 470
AluI AGCT 4 cut(s) 14, 67, 91, 470
Alw21I GWGCWC 1 cut(s) 69
Alw26I GTCTC 3 cut(s) 162, 764, 891
AlwI GGATC 3 cut(s) 341, 359, 409
AoxI GGCC 2 cut(s) 33, 511
ApeKI GCWGC 1 cut(s) 5
Asp700I GAANNNNTTC 1 cut(s) 141
AspA2I CCTAGG 1 cut(s) 494
AspLEI GCGC 1 cut(s) 415
AspS9I GGNCC 3 cut(s) 512, 710, 842
AvaII GGWCC 2 cut(s) 710, 842
AvrII CCTAGG 1 cut(s) 494
BaeI ACNNNNGTAYC 2 cut(s) 717, 750
BanII GRGCYC 1 cut(s) 69
Bbv12I GWGCWC 1 cut(s) 69
BccI CCATC 1 cut(s) 68
BciT130I CCWGG 6 cut(s) 399, 548, 614, 680, 746, 878
BclI TGATCA 2 cut(s) 43, 280
BcoDI GTCTC 3 cut(s) 162, 764, 891
BfaI CTAG 3 cut(s) 272, 495, 922
BfmI CTRYAG 5 cut(s) 522, 588, 654, 720, 852
BfoI RGCGCY 1 cut(s) 416
BfuAI ACCTGC 1 cut(s) 859
BisI GCNGC 1 cut(s) 6
BlnI CCTAGG 1 cut(s) 494
BlsI GCNGC 1 cut(s) 7
Bme1390I CCNGG 6 cut(s) 399, 548, 614, 680, 746, 878
Bme18I GGWCC 2 cut(s) 710, 842
BmgT120I GGNCC 3 cut(s) 512, 710, 842
BmrFI CCNGG 6 cut(s) 399, 548, 614, 680, 746, 878
BmsI GCATC 2 cut(s) 439, 461
BpmI CTGGAG 1 cut(s) 184
BpuEI CTTGAG 1 cut(s) 53
BsaAI YACGTR 1 cut(s) 336
BsaJI CCNNGG 6 cut(s) 494, 626, 692, 745, 758, 890
BsaXI ACNNNNNCTCC 2 cut(s) 157, 187
Bsc4I CCNNNNNNNGG 1 cut(s) 428
Bse118I RCCGGY 1 cut(s) 509
Bse1I ACTGG 1 cut(s) 240
BseBI CCWGG 6 cut(s) 399, 548, 614, 680, 746, 878
BseDI CCNNGG 6 cut(s) 494, 626, 692, 745, 758, 890
BseGI GGATG 2 cut(s) 79, 454
BseLI CCNNNNNNNGG 1 cut(s) 428
BseMII CTCAG 1 cut(s) 363
BseNI ACTGG 1 cut(s) 240
BseRI GAGGAG 1 cut(s) 411
BseYI CCCAGC 2 cut(s) 60, 87
BshFI GGCC 2 cut(s) 35, 513
BsiHKAI GWGCWC 1 cut(s) 69
BsiSI CCGG 1 cut(s) 510
BslFI GGGAC 5 cut(s) 373, 512, 644, 710, 762
BslI CCNNNNNNNGG 1 cut(s) 428
BsmAI GTCTC 3 cut(s) 162, 764, 891
BsmFI GGGAC 5 cut(s) 373, 512, 644, 710, 762
BsmI GAATGC 1 cut(s) 318
BsnI GGCC 2 cut(s) 35, 513
Bsp1286I GDGCHC 1 cut(s) 69
Bsp143I GATC 5 cut(s) 43, 280, 346, 364, 401
BspACI CCGC 2 cut(s) 105, 155
BspANI GGCC 2 cut(s) 35, 513
BspCNI CTCAG 1 cut(s) 364
BspMAI CTGCAG 1 cut(s) 856
BspMI ACCTGC 1 cut(s) 859
BspPI GGATC 3 cut(s) 341, 359, 409
BsrFI RCCGGY 1 cut(s) 509
BsrI ACTGG 1 cut(s) 240
BssAI RCCGGY 1 cut(s) 509
BssECI CCNNGG 6 cut(s) 494, 626, 692, 745, 758, 890
BssMI GATC 5 cut(s) 43, 280, 346, 364, 401
BssNAI GTATAC 1 cut(s) 339
BssT1I CCWWGG 4 cut(s) 494, 626, 692, 758
Bst1107I GTATAC 1 cut(s) 339
Bst2UI CCWGG 6 cut(s) 399, 548, 614, 680, 746, 878
Bst4CI ACNGT 3 cut(s) 173, 355, 885
BstAPI GCANNNNNTGC 2 cut(s) 102, 329
BstBAI YACGTR 1 cut(s) 336
BstC8I GCNNGC 1 cut(s) 417
BstDEI CTNAG 1 cut(s) 372
BstF5I GGATG 2 cut(s) 79, 454
BstH2I RGCGCY 1 cut(s) 416
BstHHI GCGC 1 cut(s) 415
BstKTI GATC 5 cut(s) 46, 283, 349, 367, 404
BstMAI GTCTC 3 cut(s) 162, 764, 891
BstMBI GATC 5 cut(s) 43, 280, 346, 364, 401
BstMWI GCNNNNNNNGC 5 cut(s) 11, 27, 102, 264, 329
BstNI CCWGG 6 cut(s) 399, 548, 614, 680, 746, 878
BstSCI CCNGG 6 cut(s) 397, 546, 612, 678, 744, 876
BstSFI CTRYAG 5 cut(s) 522, 588, 654, 720, 852
BstX2I RGATCY 2 cut(s) 346, 401
BstYI RGATCY 2 cut(s) 346, 401
BstZ17I GTATAC 1 cut(s) 339
BsuRI GGCC 2 cut(s) 35, 513
BtsCI GGATG 2 cut(s) 79, 454
BtsIMutI CAGTG 1 cut(s) 233
BveI ACCTGC 1 cut(s) 859
Cac8I GCNNGC 1 cut(s) 417
CfoI GCGC 1 cut(s) 415
Cfr10I RCCGGY 1 cut(s) 509
Cfr13I GGNCC 3 cut(s) 512, 710, 842
CviAII CATG 3 cut(s) 148, 216, 898
DdeI CTNAG 1 cut(s) 372
DpnI GATC 5 cut(s) 45, 282, 348, 366, 403
DpnII GATC 5 cut(s) 43, 280, 346, 364, 401
Ecl136II GAGCTC 1 cut(s) 67
Eco130I CCWWGG 4 cut(s) 494, 626, 692, 758
Eco24I GRGCYC 1 cut(s) 69
Eco47I GGWCC 2 cut(s) 710, 842
Eco53kI GAGCTC 1 cut(s) 67
Eco57I CTGAAG 1 cut(s) 279
EcoICRI GAGCTC 1 cut(s) 67
EcoRII CCWGG 6 cut(s) 397, 546, 612, 678, 744, 876
EcoT14I CCWWGG 4 cut(s) 494, 626, 692, 758
EcoT22I ATGCAT 1 cut(s) 454
EcoT38I GRGCYC 1 cut(s) 69
ErhI CCWWGG 4 cut(s) 494, 626, 692, 758
FaeI CATG 3 cut(s) 151, 219, 901
FalI AAGNNNNNCTT 2 cut(s) 766, 798
FaqI GGGAC 5 cut(s) 373, 512, 644, 710, 762
FatI CATG 3 cut(s) 147, 215, 897
FbaI TGATCA 2 cut(s) 43, 280
FblI GTMKAC 2 cut(s) 338, 543
Fnu4HI GCNGC 1 cut(s) 6
FokI GGATG 2 cut(s) 86, 461
FriOI GRGCYC 1 cut(s) 69
Fsp4HI GCNGC 1 cut(s) 6
FspBI CTAG 3 cut(s) 272, 495, 922
GlaI GCGC 1 cut(s) 414
GluI GCNGC 1 cut(s) 6
GsaI CCCAGC 2 cut(s) 64, 91
GsuI CTGGAG 1 cut(s) 184
HaeII RGCGCY 1 cut(s) 416
HaeIII GGCC 2 cut(s) 35, 513
HapII CCGG 1 cut(s) 510
HhaI GCGC 1 cut(s) 415
Hin1II CATG 3 cut(s) 151, 219, 901
Hin6I GCGC 1 cut(s) 413
HinP1I GCGC 1 cut(s) 413
HindIII AAGCTT 1 cut(s) 12
HinfI GANTC 1 cut(s) 386
HpaII CCGG 1 cut(s) 510
Hpy166II GTNNAC 2 cut(s) 339, 544
Hpy188I TCNGA 9 cut(s) 245, 364, 537, 603, 669, 801, 808, 891, 912
Hpy8I GTNNAC 2 cut(s) 339, 544
HpyAV CCTTC 1 cut(s) 152
HpyCH4III ACNGT 3 cut(s) 173, 355, 885
HpyCH4IV ACGT 1 cut(s) 335
HpyCH4V TGCA 7 cut(s) 30, 96, 207, 219, 230, 452, 854
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 27, 102, 264, 329
HpyF3I CTNAG 1 cut(s) 372
HpySE526I ACGT 1 cut(s) 335
Hsp92II CATG 3 cut(s) 151, 219, 901
HspAI GCGC 1 cut(s) 413
Ksp22I TGATCA 2 cut(s) 43, 280
Kzo9I GATC 5 cut(s) 43, 280, 346, 364, 401
LmnI GCTCC 2 cut(s) 64, 424
LweI GCATC 2 cut(s) 439, 461
MaeI CTAG 3 cut(s) 272, 495, 922
MaeII ACGT 1 cut(s) 335
MaeIII GTNAC 1 cut(s) 592
MalI GATC 5 cut(s) 45, 282, 348, 366, 403
MboI GATC 5 cut(s) 43, 280, 346, 364, 401
MboII GAAGA 2 cut(s) 396, 446
MflI RGATCY 2 cut(s) 346, 401
MhlI GDGCHC 1 cut(s) 69
MlyI GAGTC 1 cut(s) 395
MmeI TCCRAC 6 cut(s) 374, 671, 737, 779, 803, 869
MnlI CCTC 3 cut(s) 239, 432, 885
Mph1103I ATGCAT 1 cut(s) 454
MroXI GAANNNNTTC 1 cut(s) 141
MseI TTAA 2 cut(s) 54, 867
MspI CCGG 1 cut(s) 510
MspR9I CCNGG 6 cut(s) 399, 548, 614, 680, 746, 878
Mva1269I GAATGC 1 cut(s) 318
MvaI CCWGG 6 cut(s) 399, 548, 614, 680, 746, 878
MwoI GCNNNNNNNGC 5 cut(s) 11, 27, 102, 264, 329
NdeII GATC 5 cut(s) 43, 280, 346, 364, 401
NlaIII CATG 3 cut(s) 151, 219, 901
NmeAIII GCCGAG 1 cut(s) 61
NsiI ATGCAT 1 cut(s) 454
PaqCI CACCTGC 1 cut(s) 859
PctI GAATGC 1 cut(s) 318
PdmI GAANNNNTTC 1 cut(s) 141
PfoI TCCNGGA 1 cut(s) 397
PkrI GCNGC 1 cut(s) 7
PleI GAGTC 1 cut(s) 394
PpsI GAGTC 1 cut(s) 394
Ppu21I YACGTR 1 cut(s) 336
Psp124BI GAGCTC 1 cut(s) 69
Psp6I CCWGG 6 cut(s) 397, 546, 612, 678, 744, 876
PspFI CCCAGC 2 cut(s) 60, 87
PspGI CCWGG 6 cut(s) 397, 546, 612, 678, 744, 876
PspPI GGNCC 3 cut(s) 512, 710, 842
PstI CTGCAG 1 cut(s) 856
PsuI RGATCY 2 cut(s) 346, 401
SacI GAGCTC 1 cut(s) 69
SaqAI TTAA 2 cut(s) 54, 867
SatI GCNGC 1 cut(s) 6
Sau3AI GATC 5 cut(s) 43, 280, 346, 364, 401
Sau96I GGNCC 3 cut(s) 512, 710, 842
SchI GAGTC 1 cut(s) 395
ScrFI CCNGG 6 cut(s) 399, 548, 614, 680, 746, 878
SduI GDGCHC 1 cut(s) 69
SfaNI GCATC 2 cut(s) 439, 461
SfcI CTRYAG 5 cut(s) 522, 588, 654, 720, 852
SinI GGWCC 2 cut(s) 710, 842
SmlI CTYRAG 1 cut(s) 68
SmoI CTYRAG 1 cut(s) 68
SsiI CCGC 2 cut(s) 105, 155
SspMI CTAG 3 cut(s) 272, 495, 922
SstI GAGCTC 1 cut(s) 69
StyD4I CCNGG 6 cut(s) 397, 546, 612, 678, 744, 876
StyI CCWWGG 4 cut(s) 494, 626, 692, 758
TaaI ACNGT 3 cut(s) 173, 355, 885
TaiI ACGT 1 cut(s) 338
TaqI TCGA 1 cut(s) 301
Tru1I TTAA 2 cut(s) 54, 867
Tru9I TTAA 2 cut(s) 54, 867
TscAI CASTG 1 cut(s) 240
TseI GCWGC 1 cut(s) 5
TspDTI ATGAA 1 cut(s) 348
TspGWI ACGGA 1 cut(s) 461
TspRI CASTG 1 cut(s) 240
VpaK11BI GGWCC 2 cut(s) 710, 842
XmaJI CCTAGG 1 cut(s) 494
XmiI GTMKAC 2 cut(s) 338, 543
XmnI GAANNNNTTC 1 cut(s) 141
XspI CTAG 3 cut(s) 272, 495, 922
Zsp2I ATGCAT 1 cut(s) 454
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.