Rh5CG276600

B3 DNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0001196
Physical Location & Seq
Reverse (-)
30066054 .. 30067519
1466 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG276600.1

Sequence Viewer

Length: 546 bp
ATGGTAACTGACTATATCCTTTGCAAATTTAGTTTTGCTTATGTGAATGGTATATTTTGTGAAACTTTTCACGTGGAAGCGGAAGGCTGGAGAGACTGTGCTGTTTGTGGGAAGCCACTCCATTGTGGGTGCATTATGTCACTCCACAAGTATGCACCACTGGATTTTGGAGGTGTAGGTTGCACAAAATGCTTACTAGAAGTTGATCAATCTATTGCTTCAGGTCGAATGACCAGAATGCTGAAAGAAATAGTTGCCAAGTACAAGACGGACCCAACAGTTTCATCTGATCCTACTAAGAAAGCTGTCGAGTCCCTTTTTCCTGAATCTTTACAAGCTCCAGCACCTCAGATTGGAGAAGAATCCAATACGGATGTATCTAAAGGAGATACAGCTTGTGAATTCAAAGTAGGCAGTGAAACACCTCAAGTGGATGCCTCCACAGGAAACCATTTACACCCTGACAATCTGCCGAAGATGACAAGGGCTGAGCAACTCCAAAAAGTCTCCGGAAAGTATCCATTAATCTTGTGTCATAGGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

181

Amino Acids

19.88

Weight (kDa)

6.06

Isoelectric Point (pI)

40.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf_VAL1_N PF25813 22 - 66 1.4e-21 VAL1-like, N-terminal zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 509
AciI CCGC 1 cut(s) 80
AclWI GGATC 1 cut(s) 284
AcsI RAATTY 2 cut(s) 26, 401
AcuI CTGAAG 1 cut(s) 204
AcvI CACGTG 1 cut(s) 73
AfaI GTAC 1 cut(s) 263
AfiI CCNNNNNNNGG 1 cut(s) 353
AgsI TTSAA 1 cut(s) 406
AluBI AGCT 3 cut(s) 305, 338, 395
AluI AGCT 3 cut(s) 305, 338, 395
Alw26I GTCTC 2 cut(s) 87, 511
AlwI GGATC 1 cut(s) 284
Aor13HI TCCGGA 1 cut(s) 509
ApoI RAATTY 2 cut(s) 26, 401
AseI ATTAAT 1 cut(s) 524
Asp700I GAANNNNTTC 1 cut(s) 66
AspS9I GGNCC 1 cut(s) 271
AvaII GGWCC 1 cut(s) 271
BaeI ACNNNNGTAYC 2 cut(s) 360, 393
BbrPI CACGTG 1 cut(s) 73
BciVI GTATCC 1 cut(s) 528
BclI TGATCA 1 cut(s) 205
BcoDI GTCTC 2 cut(s) 87, 511
BfaI CTAG 1 cut(s) 197
BfuI GTATCC 1 cut(s) 528
BlpI GCTNAGC 1 cut(s) 489
Bme18I GGWCC 1 cut(s) 271
BmgT120I GGNCC 1 cut(s) 271
BmiI GGNNCC 1 cut(s) 273
BmsI GCATC 1 cut(s) 424
BpmI CTGGAG 2 cut(s) 109, 324
Bpu1102I GCTNAGC 1 cut(s) 489
BpuEI CTTGAG 1 cut(s) 411
BsaAI YACGTR 1 cut(s) 73
BsaWI WCCGGW 1 cut(s) 509
BsaXI ACNNNNNCTCC 2 cut(s) 82, 112
Bsc4I CCNNNNNNNGG 1 cut(s) 353
Bse1I ACTGG 1 cut(s) 165
BseAI TCCGGA 1 cut(s) 509
BseGI GGATG 2 cut(s) 379, 439
BseLI CCNNNNNNNGG 1 cut(s) 353
BseMII CTCAG 2 cut(s) 362, 480
BseNI ACTGG 1 cut(s) 165
BsiSI CCGG 1 cut(s) 510
BslFI GGGAC 1 cut(s) 298
BslI CCNNNNNNNGG 1 cut(s) 353
BsmAI GTCTC 2 cut(s) 87, 511
BsmFI GGGAC 1 cut(s) 298
BsmI GAATGC 1 cut(s) 243
Bsp13I TCCGGA 1 cut(s) 509
Bsp143I GATC 2 cut(s) 205, 289
Bsp1720I GCTNAGC 1 cut(s) 489
BspACI CCGC 1 cut(s) 80
BspCNI CTCAG 2 cut(s) 361, 481
BspEI TCCGGA 1 cut(s) 509
BspLI GGNNCC 1 cut(s) 273
BspPI GGATC 1 cut(s) 284
BsrI ACTGG 1 cut(s) 165
BssMI GATC 2 cut(s) 205, 289
Bst4CI ACNGT 2 cut(s) 98, 280
BstAPI GCANNNNNTGC 1 cut(s) 189
BstBAI YACGTR 1 cut(s) 73
BstDEI CTNAG 3 cut(s) 297, 348, 489
BstF5I GGATG 2 cut(s) 379, 439
BstKTI GATC 2 cut(s) 208, 292
BstMAI GTCTC 2 cut(s) 87, 511
BstMBI GATC 2 cut(s) 205, 289
BstMWI GCNNNNNNNGC 1 cut(s) 189
BsuI GTATCC 1 cut(s) 528
BtsCI GGATG 2 cut(s) 379, 439
BtsI GCAGTG 1 cut(s) 421
BtsIMutI CAGTG 2 cut(s) 158, 421
Cfr13I GGNCC 1 cut(s) 271
Csp6I GTAC 1 cut(s) 262
CviJI RGCY 6 cut(s) 87, 115, 305, 338, 395, 488
CviKI_1 RGCY 6 cut(s) 87, 115, 305, 338, 395, 488
CviQI GTAC 1 cut(s) 262
DdeI CTNAG 3 cut(s) 297, 348, 489
DpnI GATC 2 cut(s) 207, 291
DpnII GATC 2 cut(s) 205, 289
Eco47I GGWCC 1 cut(s) 271
Eco57I CTGAAG 1 cut(s) 204
Eco72I CACGTG 1 cut(s) 73
EcoRI GAATTC 1 cut(s) 401
FaiI YATR 6 cut(s) 15, 42, 53, 137, 153, 537
FaqI GGGAC 1 cut(s) 298
FbaI TGATCA 1 cut(s) 205
FokI GGATG 2 cut(s) 386, 446
FspBI CTAG 1 cut(s) 197
GsuI CTGGAG 2 cut(s) 109, 324
HapII CCGG 1 cut(s) 510
HinfI GANTC 3 cut(s) 311, 326, 362
HpaII CCGG 1 cut(s) 510
Hpy188I TCNGA 2 cut(s) 289, 351
Hpy188III TCNNGA 2 cut(s) 323, 510
HpyAV CCTTC 1 cut(s) 77
HpyCH4III ACNGT 2 cut(s) 98, 280
HpyCH4IV ACGT 1 cut(s) 72
HpyCH4V TGCA 4 cut(s) 24, 132, 155, 183
HpyF10VI GCNNNNNNNGC 1 cut(s) 189
HpyF3I CTNAG 3 cut(s) 297, 348, 489
HpySE526I ACGT 1 cut(s) 72
Kpn2I TCCGGA 1 cut(s) 509
Ksp22I TGATCA 1 cut(s) 205
Kzo9I GATC 2 cut(s) 205, 289
LmnI GCTCC 1 cut(s) 343
LpnPI CCDG 9 cut(s) 73, 146, 207, 247, 336, 354, 429, 474, 523
LweI GCATC 1 cut(s) 424
MaeI CTAG 1 cut(s) 197
MaeII ACGT 1 cut(s) 72
MaeIII GTNAC 2 cut(s) 4, 138
MalI GATC 2 cut(s) 207, 291
MboI GATC 2 cut(s) 205, 289
MboII GAAGA 2 cut(s) 371, 487
MluCI AATT 2 cut(s) 26, 401
MlyI GAGTC 1 cut(s) 320
MnlI CCTC 4 cut(s) 164, 357, 435, 448
MroI TCCGGA 1 cut(s) 509
MroXI GAANNNNTTC 1 cut(s) 66
MseI TTAA 1 cut(s) 524
MslI CAYNNNNRTG 1 cut(s) 150
MspI CCGG 1 cut(s) 510
Mva1269I GAATGC 1 cut(s) 243
MwoI GCNNNNNNNGC 1 cut(s) 189
NdeII GATC 2 cut(s) 205, 289
NlaIV GGNNCC 1 cut(s) 273
NmuCI GTSAC 1 cut(s) 138
PctI GAATGC 1 cut(s) 243
PdmI GAANNNNTTC 1 cut(s) 66
PfeI GAWTC 2 cut(s) 326, 362
PleI GAGTC 1 cut(s) 319
PmaCI CACGTG 1 cut(s) 73
PmlI CACGTG 1 cut(s) 73
PpsI GAGTC 1 cut(s) 319
Ppu21I YACGTR 1 cut(s) 73
PshBI ATTAAT 1 cut(s) 524
PspCI CACGTG 1 cut(s) 73
PspN4I GGNNCC 1 cut(s) 273
PspPI GGNCC 1 cut(s) 271
RsaI GTAC 1 cut(s) 263
RsaNI GTAC 1 cut(s) 262
RseI CAYNNNNRTG 1 cut(s) 150
SaqAI TTAA 1 cut(s) 524
Sau3AI GATC 2 cut(s) 205, 289
Sau96I GGNCC 1 cut(s) 271
SchI GAGTC 1 cut(s) 320
SfaNI GCATC 1 cut(s) 424
SinI GGWCC 1 cut(s) 271
SmiMI CAYNNNNRTG 1 cut(s) 150
SmlI CTYRAG 1 cut(s) 426
SmoI CTYRAG 1 cut(s) 426
Sse9I AATT 2 cut(s) 26, 401
SsiI CCGC 1 cut(s) 80
SspMI CTAG 1 cut(s) 197
TaaI ACNGT 2 cut(s) 98, 280
TaiI ACGT 1 cut(s) 75
TaqI TCGA 2 cut(s) 226, 309
TasI AATT 2 cut(s) 26, 401
TatI WGTACW 1 cut(s) 261
TfiI GAWTC 2 cut(s) 326, 362
Tru1I TTAA 1 cut(s) 524
Tru9I TTAA 1 cut(s) 524
TscAI CASTG 2 cut(s) 165, 421
TseFI GTSAC 1 cut(s) 138
Tsp45I GTSAC 1 cut(s) 138
TspDTI ATGAA 1 cut(s) 273
TspGWI ACGGA 2 cut(s) 284, 386
TspRI CASTG 2 cut(s) 165, 421
VpaK11BI GGWCC 1 cut(s) 271
VspI ATTAAT 1 cut(s) 524
XapI RAATTY 2 cut(s) 26, 401
XmnI GAANNNNTTC 1 cut(s) 66
XspI CTAG 1 cut(s) 197
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.