Rw5G022440

B3 DNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0001196
Physical Location & Seq
Reverse (-)
30960778 .. 30967382
6605 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G022440.1

Sequence Viewer

Length: 1155 bp
ATGGCTGCCAAAGCTTCGTGCTTTTCCTGCAAGGTCGAGAGTGATCAGCTTGTTAATGGCTGGGAGCTCAAGGATGGGTCTTTCGCCCAGCTTTGCAAGAAATGCGGTTTTGCTTATGTGAATGGTATATTTTGTGAAACTTTTCACATGGAAGCAGACAGCTGGAGAGACTGTGCTGTTTGTGGGAAGCCACTCCATTGTGGGTGCATTATGTCACGCCACAAGTATGCACCACTGGATTTCGGAGGTGTAGGTTGCACAAAATGCTTACTAGAAGTTGATCAATCTATTGCTTCAGGTCGAATGACCAGAATGGTGAAAGACATAGTTGCCAAGTACAAGACGGACCCAACAGTTTCATCTGATCCTACTAAGAAAGTTGTTGAGTCCCTTTTTCCTGAATCTTTACAAGCTCCAGCACCTCAGATTGGAGAAGAATCCAATACGGATGTATCTAAAGGAGATACAGCTTGTGAATTCAAAGTAGACAGTGAAAAACCTCAAGTGGATGCCTCCACAGGAAACCATTCACACCCTGACAATCTGCTGAAGATGACAAGGGCTGAGCAACTCCAAAAAAAGTCATCAACCGTAAGTGATGCTAATCACAGTACAGGATTTCTTGTGATACCGAAGAAATATGCAGAGGAACAATCTCCAAGGGACATTGAAACAGGTCCAACACCTGTATATACAAGTCTGAGAGCAGACCAGGAACAATCTCCAATGGATGTGGTAACAAATCCAATGCCTATAGAAACAAATCAGAAAACAAACCAGGAACAATCTCCAAGGGACATTGAAACAGGTCCAACGCGTGTAGATACAACCCTGAAACCAGACCAGGAACAATCTCCGATGGTAATGGAAACAAGTCCAATGCCTATAGAAACAAATCAGAAAACGAACCACGAACAATCTCTAAGGGACTTTGAAACAGGTCCAACGTTTGTAGATACAAGTCTGAAAACAGACCAGGAACAACCTTCAAAGGATATGGAGACAAGTCCAATGCCTGTAGAAACAAGTCTTAAATCAGACCAGGGACAATCTCCAAAGGATATGGAAACAGGTCCAACGCCTGTAGAATCAAGTCTGAAAACAGACCAGGAACAGTCTCCCAAGGGTATGGAAACAAGTCCGAAAACAGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

384

Amino Acids

42.24

Weight (kDa)

4.88

Isoelectric Point (pI)

54.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf_VAL1_N PF25813 47 - 91 6.2e-20 VAL1-like, N-terminal zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 486
AccII CGCG 1 cut(s) 817
AciI CCGC 1 cut(s) 105
AclI AACGTT 1 cut(s) 947
AclWI GGATC 1 cut(s) 359
AcsI RAATTY 1 cut(s) 476
AcuI CTGAAG 2 cut(s) 279, 569
AfaI GTAC 2 cut(s) 338, 613
AfiI CCNNNNNNNGG 1 cut(s) 428
AflIII ACRYGT 1 cut(s) 815
AgsI TTSAA 5 cut(s) 481, 671, 803, 935, 990
AjnI CCWGG 6 cut(s) 711, 777, 843, 975, 1041, 1107
AluBI AGCT 7 cut(s) 14, 49, 67, 91, 162, 413, 470
AluI AGCT 7 cut(s) 14, 49, 67, 91, 162, 413, 470
Alw21I GWGCWC 1 cut(s) 69
Alw26I GTCTC 3 cut(s) 162, 995, 1122
AlwI GGATC 1 cut(s) 359
ApeKI GCWGC 1 cut(s) 5
ApoI RAATTY 1 cut(s) 476
Asp700I GAANNNNTTC 2 cut(s) 141, 526
AspS9I GGNCC 5 cut(s) 346, 677, 809, 941, 1073
AsuHPI GGTGA 1 cut(s) 328
AvaII GGWCC 5 cut(s) 346, 677, 809, 941, 1073
BaeI ACNNNNGTAYC 2 cut(s) 435, 468
BanII GRGCYC 1 cut(s) 69
Bbv12I GWGCWC 1 cut(s) 69
BccI CCATC 2 cut(s) 68, 853
BciT130I CCWGG 6 cut(s) 713, 779, 845, 977, 1043, 1109
BclI TGATCA 2 cut(s) 43, 280
BcoDI GTCTC 3 cut(s) 162, 995, 1122
BfaI CTAG 2 cut(s) 272, 1153
BfmI CTRYAG 4 cut(s) 753, 885, 1017, 1083
BisI GCNGC 1 cut(s) 6
BlpI GCTNAGC 1 cut(s) 564
BlsI GCNGC 1 cut(s) 7
Bme1390I CCNGG 6 cut(s) 713, 779, 845, 977, 1043, 1109
Bme18I GGWCC 5 cut(s) 346, 677, 809, 941, 1073
BmgT120I GGNCC 5 cut(s) 346, 677, 809, 941, 1073
BmiI GGNNCC 1 cut(s) 348
BmrFI CCNGG 6 cut(s) 713, 779, 845, 977, 1043, 1109
BmsI GCATC 2 cut(s) 499, 589
BpmI CTGGAG 2 cut(s) 184, 399
Bpu1102I GCTNAGC 1 cut(s) 564
BpuEI CTTGAG 2 cut(s) 53, 486
BsaBI GATNNNNATC 1 cut(s) 603
BsaJI CCNNGG 4 cut(s) 659, 791, 1042, 1122
BsaXI ACNNNNNCTCC 2 cut(s) 157, 187
Bsc4I CCNNNNNNNGG 1 cut(s) 428
Bse1I ACTGG 1 cut(s) 240
Bse8I GATNNNNATC 1 cut(s) 603
BseBI CCWGG 6 cut(s) 713, 779, 845, 977, 1043, 1109
BseDI CCNNGG 4 cut(s) 659, 791, 1042, 1122
BseGI GGATG 4 cut(s) 79, 454, 514, 736
BseJI GATNNNNATC 1 cut(s) 603
BseLI CCNNNNNNNGG 1 cut(s) 428
BseMII CTCAG 3 cut(s) 437, 555, 692
BseNI ACTGG 1 cut(s) 240
BseYI CCCAGC 2 cut(s) 60, 87
Bsh1236I CGCG 1 cut(s) 817
BsiHKAI GWGCWC 1 cut(s) 69
BslFI GGGAC 5 cut(s) 373, 677, 809, 941, 1059
BslI CCNNNNNNNGG 1 cut(s) 428
BsmAI GTCTC 3 cut(s) 162, 995, 1122
BsmFI GGGAC 5 cut(s) 373, 677, 809, 941, 1059
Bsp1286I GDGCHC 1 cut(s) 69
Bsp143I GATC 3 cut(s) 43, 280, 364
Bsp1720I GCTNAGC 1 cut(s) 564
BspACI CCGC 1 cut(s) 105
BspCNI CTCAG 3 cut(s) 436, 556, 693
BspFNI CGCG 1 cut(s) 817
BspLI GGNNCC 1 cut(s) 348
BspPI GGATC 1 cut(s) 359
BsrI ACTGG 1 cut(s) 240
BssECI CCNNGG 4 cut(s) 659, 791, 1042, 1122
BssMI GATC 3 cut(s) 43, 280, 364
BssT1I CCWWGG 3 cut(s) 659, 791, 1122
Bst2UI CCWGG 6 cut(s) 713, 779, 845, 977, 1043, 1109
Bst4CI ACNGT 6 cut(s) 173, 355, 491, 592, 611, 1116
BstAPI GCANNNNNTGC 2 cut(s) 102, 264
BstDEI CTNAG 5 cut(s) 372, 423, 564, 701, 923
BstF5I GGATG 4 cut(s) 79, 454, 514, 736
BstFNI CGCG 1 cut(s) 817
BstKTI GATC 3 cut(s) 46, 283, 367
BstMAI GTCTC 3 cut(s) 162, 995, 1122
BstMBI GATC 3 cut(s) 43, 280, 364
BstMWI GCNNNNNNNGC 4 cut(s) 11, 27, 102, 264
BstNI CCWGG 6 cut(s) 713, 779, 845, 977, 1043, 1109
BstSCI CCNGG 6 cut(s) 711, 777, 843, 975, 1041, 1107
BstSFI CTRYAG 4 cut(s) 753, 885, 1017, 1083
BstUI CGCG 1 cut(s) 817
BstXI CCANNNNNNTGG 1 cut(s) 1129
BtsCI GGATG 4 cut(s) 79, 454, 514, 736
BtsIMutI CAGTG 2 cut(s) 233, 496
Cfr13I GGNCC 5 cut(s) 346, 677, 809, 941, 1073
Csp6I GTAC 2 cut(s) 337, 612
CspCI CAANNNNNGTGG 2 cut(s) 714, 749
CviAII CATG 1 cut(s) 148
CviQI GTAC 2 cut(s) 337, 612
DdeI CTNAG 5 cut(s) 372, 423, 564, 701, 923
DpnI GATC 3 cut(s) 45, 282, 366
DpnII GATC 3 cut(s) 43, 280, 364
Ecl136II GAGCTC 1 cut(s) 67
Eco130I CCWWGG 3 cut(s) 659, 791, 1122
Eco24I GRGCYC 1 cut(s) 69
Eco47I GGWCC 5 cut(s) 346, 677, 809, 941, 1073
Eco53kI GAGCTC 1 cut(s) 67
Eco57I CTGAAG 2 cut(s) 279, 569
EcoICRI GAGCTC 1 cut(s) 67
EcoRI GAATTC 1 cut(s) 476
EcoRII CCWGG 6 cut(s) 711, 777, 843, 975, 1041, 1107
EcoT14I CCWWGG 3 cut(s) 659, 791, 1122
EcoT38I GRGCYC 1 cut(s) 69
ErhI CCWWGG 3 cut(s) 659, 791, 1122
FaeI CATG 1 cut(s) 151
FaqI GGGAC 5 cut(s) 373, 677, 809, 941, 1059
FatI CATG 1 cut(s) 147
FbaI TGATCA 2 cut(s) 43, 280
FblI GTMKAC 1 cut(s) 486
Fnu4HI GCNGC 1 cut(s) 6
FokI GGATG 4 cut(s) 86, 461, 521, 743
FriOI GRGCYC 1 cut(s) 69
Fsp4HI GCNGC 1 cut(s) 6
FspBI CTAG 2 cut(s) 272, 1153
GluI GCNGC 1 cut(s) 6
GsaI CCCAGC 2 cut(s) 64, 91
GsuI CTGGAG 2 cut(s) 184, 399
Hin1II CATG 1 cut(s) 151
HindIII AAGCTT 1 cut(s) 12
HinfI GANTC 4 cut(s) 386, 401, 437, 1088
HphI GGTGA 1 cut(s) 328
Hpy166II GTNNAC 1 cut(s) 487
Hpy188III TCNNGA 2 cut(s) 37, 398
Hpy8I GTNNAC 1 cut(s) 487
HpyAV CCTTC 1 cut(s) 996
HpyCH4III ACNGT 6 cut(s) 173, 355, 491, 592, 611, 1116
HpyCH4IV ACGT 1 cut(s) 947
HpyCH4V TGCA 6 cut(s) 30, 96, 207, 230, 258, 644
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 27, 102, 264
HpyF3I CTNAG 5 cut(s) 372, 423, 564, 701, 923
HpySE526I ACGT 1 cut(s) 947
Hsp92II CATG 1 cut(s) 151
Ksp22I TGATCA 2 cut(s) 43, 280
Kzo9I GATC 3 cut(s) 43, 280, 364
LmnI GCTCC 2 cut(s) 64, 418
LweI GCATC 2 cut(s) 499, 589
MaeI CTAG 2 cut(s) 272, 1153
MaeII ACGT 1 cut(s) 947
MaeIII GTNAC 2 cut(s) 213, 736
MalI GATC 3 cut(s) 45, 282, 366
MboI GATC 3 cut(s) 43, 280, 364
MboII GAAGA 3 cut(s) 446, 562, 646
MhlI GDGCHC 1 cut(s) 69
MluCI AATT 1 cut(s) 476
MluI ACGCGT 1 cut(s) 815
MlyI GAGTC 1 cut(s) 395
MmeI TCCRAC 4 cut(s) 704, 836, 968, 1100
MnlI CCTC 5 cut(s) 239, 432, 510, 523, 640
MroXI GAANNNNTTC 2 cut(s) 141, 526
MseI TTAA 2 cut(s) 54, 1032
MslI CAYNNNNRTG 1 cut(s) 225
MspA1I CMGCKG 1 cut(s) 162
MspR9I CCNGG 6 cut(s) 713, 779, 845, 977, 1043, 1109
MvaI CCWGG 6 cut(s) 713, 779, 845, 977, 1043, 1109
MvnI CGCG 1 cut(s) 817
MwoI GCNNNNNNNGC 4 cut(s) 11, 27, 102, 264
NdeII GATC 3 cut(s) 43, 280, 364
NlaIII CATG 1 cut(s) 151
NlaIV GGNNCC 1 cut(s) 348
NmuCI GTSAC 1 cut(s) 213
PdmI GAANNNNTTC 2 cut(s) 141, 526
PfeI GAWTC 3 cut(s) 401, 437, 1088
PkrI GCNGC 1 cut(s) 7
PleI GAGTC 1 cut(s) 394
PpsI GAGTC 1 cut(s) 394
Psp124BI GAGCTC 1 cut(s) 69
Psp1406I AACGTT 1 cut(s) 947
Psp6I CCWGG 6 cut(s) 711, 777, 843, 975, 1041, 1107
PspFI CCCAGC 2 cut(s) 60, 87
PspGI CCWGG 6 cut(s) 711, 777, 843, 975, 1041, 1107
PspN4I GGNNCC 1 cut(s) 348
PspPI GGNCC 5 cut(s) 346, 677, 809, 941, 1073
PvuII CAGCTG 1 cut(s) 162
RsaI GTAC 2 cut(s) 338, 613
RsaNI GTAC 2 cut(s) 337, 612
RseI CAYNNNNRTG 1 cut(s) 225
SacI GAGCTC 1 cut(s) 69
SaqAI TTAA 2 cut(s) 54, 1032
SatI GCNGC 1 cut(s) 6
Sau3AI GATC 3 cut(s) 43, 280, 364
Sau96I GGNCC 5 cut(s) 346, 677, 809, 941, 1073
SchI GAGTC 1 cut(s) 395
ScrFI CCNGG 6 cut(s) 713, 779, 845, 977, 1043, 1109
SduI GDGCHC 1 cut(s) 69
SfaNI GCATC 2 cut(s) 499, 589
SfcI CTRYAG 4 cut(s) 753, 885, 1017, 1083
SinI GGWCC 5 cut(s) 346, 677, 809, 941, 1073
SmiMI CAYNNNNRTG 1 cut(s) 225
SmlI CTYRAG 2 cut(s) 68, 501
SmoI CTYRAG 2 cut(s) 68, 501
Sse9I AATT 1 cut(s) 476
SsiI CCGC 1 cut(s) 105
SspMI CTAG 2 cut(s) 272, 1153
SstI GAGCTC 1 cut(s) 69
StyD4I CCNGG 6 cut(s) 711, 777, 843, 975, 1041, 1107
StyI CCWWGG 3 cut(s) 659, 791, 1122
TaaI ACNGT 6 cut(s) 173, 355, 491, 592, 611, 1116
TaiI ACGT 1 cut(s) 950
TaqI TCGA 2 cut(s) 36, 301
TasI AATT 1 cut(s) 476
TatI WGTACW 2 cut(s) 336, 611
TfiI GAWTC 3 cut(s) 401, 437, 1088
Tru1I TTAA 2 cut(s) 54, 1032
Tru9I TTAA 2 cut(s) 54, 1032
TscAI CASTG 2 cut(s) 240, 496
TseFI GTSAC 1 cut(s) 213
TseI GCWGC 1 cut(s) 5
Tsp45I GTSAC 1 cut(s) 213
TspDTI ATGAA 1 cut(s) 348
TspGWI ACGGA 2 cut(s) 359, 461
TspRI CASTG 2 cut(s) 240, 496
VpaK11BI GGWCC 5 cut(s) 346, 677, 809, 941, 1073
XapI RAATTY 1 cut(s) 476
XmiI GTMKAC 1 cut(s) 486
XmnI GAANNNNTTC 2 cut(s) 141, 526
XspI CTAG 2 cut(s) 272, 1153
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.