Rh5CG276000

B3 DNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0001196
Physical Location & Seq
Reverse (-)
29996420 .. 30000197
3778 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG276000.1

Sequence Viewer

Length: 972 bp
ATGGACGCGGAAGGCTGGAGAGACTGTGCTGTTTGTGGGAAGCCACTCCATCGTGGGTGCATTATGTCATGCAACAAGTATGCACCACTGGATTTCGGAGGTGTAGGTTGCTCAAAATGCTTACTAGAAGTTGATCAGTCTATTGCGTCAGGTCGAATGACCAGAATGCTGAAAGAAATAGTTGCCACGTATAAGACAGATCCAACAGTTTCATCTGATCCTGCTGAGAAAGTTGTCGAGTCCCTTTCTCCTGGATCTTCACAAGCGCCTGCTCCTCATTTTGGAGAAGAAGCCAATACGGATGCATCTAAAGGAGATACAGCTTGTGATACCAAAGTAGACAGTGAAACACCTCAAGTGGATATCTCCACAGGAAACCATTTACACCCTGACAATCTGCCGAAGATGACAAGTGCTGAGCAACTCCAAAAAGTCTCCGGAAACTTAAACTCTGATCTTATACCTCTGTTTGAGAAGTCATCAAACACAAGTGATTCTAATCGTAGTACAGGATGTCTTGTGATACCAAAGAAATACGCAGAGGAACAATCTCCTAGGGACATTGAAACCGGCCCTACGCCTGTAGATACAAGTCTGAAAGTAGACCAGGAACAATCTCCGATGGATATGGAAACAAGTCCAATGCCTATAGTAACAAATCAGAAAACAAACCAGGAACAATCTCCAAGGGACATTGAAACAAGTCCAACACCTGTAGATACAAGTCTGAAAACAGATCAGGAACAAACTCCAAGGGACATTGAAACAGGTCCAACGCCTGTAGATACAAGTGTGAAAACAGACCAGGGACAATCTCCAAGGGATATGGAGACAAGTCCAACTTTTGTAGAAGCAAGTTGGAAATCAGACGAGGAACAATCTCAAAGGGATATGGAAACAGGTCCAACACCTGCAGAAACAACCCTTAAAACAGACCAGGAACAGTCTCCGATGGGTATGAAAACAGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

323

Amino Acids

35.03

Weight (kDa)

4.47

Isoelectric Point (pI)

54.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf_VAL1_N PF25813 2 - 42 2e-16 VAL1-like, N-terminal zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 919
Acc36I ACCTGC 1 cut(s) 919
AccI GTMKAC 2 cut(s) 339, 603
AccII CGCG 1 cut(s) 8
AccIII TCCGGA 1 cut(s) 437
AciI CCGC 1 cut(s) 8
AclWI GGATC 3 cut(s) 194, 212, 262
AfaI GTAC 1 cut(s) 508
AfiI CCNNNNNNNGG 1 cut(s) 281
AgsI TTSAA 3 cut(s) 566, 698, 764
AjnI CCWGG 5 cut(s) 250, 606, 672, 804, 936
AluBI AGCT 1 cut(s) 323
AluI AGCT 1 cut(s) 323
Alw26I GTCTC 4 cut(s) 15, 439, 824, 951
AlwI GGATC 3 cut(s) 194, 212, 262
Aor13HI TCCGGA 1 cut(s) 437
AoxI GGCC 1 cut(s) 571
AspA2I CCTAGG 1 cut(s) 554
AspLEI GCGC 1 cut(s) 268
AspS9I GGNCC 3 cut(s) 572, 770, 902
AvaII GGWCC 2 cut(s) 770, 902
AvrII CCTAGG 1 cut(s) 554
BaeI ACNNNNGTAYC 4 cut(s) 321, 354, 777, 810
BccI CCATC 3 cut(s) 57, 616, 946
BciT130I CCWGG 5 cut(s) 252, 608, 674, 806, 938
BclI TGATCA 1 cut(s) 133
BcoDI GTCTC 4 cut(s) 15, 439, 824, 951
BfaI CTAG 3 cut(s) 125, 555, 970
BfmI CTRYAG 5 cut(s) 582, 648, 714, 780, 912
BfoI RGCGCY 1 cut(s) 269
BfuAI ACCTGC 1 cut(s) 919
BlnI CCTAGG 1 cut(s) 554
BlpI GCTNAGC 1 cut(s) 417
Bme1390I CCNGG 5 cut(s) 252, 608, 674, 806, 938
Bme18I GGWCC 2 cut(s) 770, 902
BmgT120I GGNCC 3 cut(s) 572, 770, 902
BmrFI CCNGG 5 cut(s) 252, 608, 674, 806, 938
BmsI GCATC 2 cut(s) 292, 314
BpmI CTGGAG 1 cut(s) 37
Bpu1102I GCTNAGC 1 cut(s) 417
BpuEI CTTGAG 1 cut(s) 339
BsaAI YACGTR 1 cut(s) 189
BsaBI GATNNNNATC 1 cut(s) 498
BsaJI CCNNGG 5 cut(s) 554, 686, 752, 805, 818
BsaWI WCCGGW 1 cut(s) 437
BsaXI ACNNNNNCTCC 2 cut(s) 10, 40
Bsc4I CCNNNNNNNGG 1 cut(s) 281
Bse118I RCCGGY 1 cut(s) 569
Bse1I ACTGG 1 cut(s) 93
Bse8I GATNNNNATC 1 cut(s) 498
BseAI TCCGGA 1 cut(s) 437
BseBI CCWGG 5 cut(s) 252, 608, 674, 806, 938
BseDI CCNNGG 5 cut(s) 554, 686, 752, 805, 818
BseGI GGATG 2 cut(s) 307, 518
BseJI GATNNNNATC 1 cut(s) 498
BseLI CCNNNNNNNGG 1 cut(s) 281
BseMII CTCAG 2 cut(s) 216, 408
BseNI ACTGG 1 cut(s) 93
BseRI GAGGAG 1 cut(s) 264
Bsh1236I CGCG 1 cut(s) 8
BshFI GGCC 1 cut(s) 573
BsiSI CCGG 2 cut(s) 438, 570
BslFI GGGAC 5 cut(s) 226, 572, 704, 770, 822
BslI CCNNNNNNNGG 1 cut(s) 281
BsmAI GTCTC 4 cut(s) 15, 439, 824, 951
BsmFI GGGAC 5 cut(s) 226, 572, 704, 770, 822
BsmI GAATGC 1 cut(s) 171
BsnI GGCC 1 cut(s) 573
Bsp13I TCCGGA 1 cut(s) 437
Bsp143I GATC 6 cut(s) 133, 199, 217, 254, 454, 736
Bsp1720I GCTNAGC 1 cut(s) 417
BspACI CCGC 1 cut(s) 8
BspANI GGCC 1 cut(s) 573
BspCNI CTCAG 2 cut(s) 217, 409
BspEI TCCGGA 1 cut(s) 437
BspFNI CGCG 1 cut(s) 8
BspMAI CTGCAG 1 cut(s) 916
BspMI ACCTGC 1 cut(s) 919
BspPI GGATC 3 cut(s) 194, 212, 262
BsrFI RCCGGY 1 cut(s) 569
BsrI ACTGG 1 cut(s) 93
BssAI RCCGGY 1 cut(s) 569
BssECI CCNNGG 5 cut(s) 554, 686, 752, 805, 818
BssMI GATC 6 cut(s) 133, 199, 217, 254, 454, 736
BssT1I CCWWGG 4 cut(s) 554, 686, 752, 818
Bst2UI CCWGG 5 cut(s) 252, 608, 674, 806, 938
Bst4CI ACNGT 4 cut(s) 26, 208, 344, 945
BstBAI YACGTR 1 cut(s) 189
BstC8I GCNNGC 1 cut(s) 270
BstDEI CTNAG 2 cut(s) 225, 417
BstF5I GGATG 2 cut(s) 307, 518
BstFNI CGCG 1 cut(s) 8
BstH2I RGCGCY 1 cut(s) 269
BstHHI GCGC 1 cut(s) 268
BstKTI GATC 6 cut(s) 136, 202, 220, 257, 457, 739
BstMAI GTCTC 4 cut(s) 15, 439, 824, 951
BstMBI GATC 6 cut(s) 133, 199, 217, 254, 454, 736
BstMWI GCNNNNNNNGC 1 cut(s) 117
BstNI CCWGG 5 cut(s) 252, 608, 674, 806, 938
BstSCI CCNGG 5 cut(s) 250, 606, 672, 804, 936
BstSFI CTRYAG 5 cut(s) 582, 648, 714, 780, 912
BstUI CGCG 1 cut(s) 8
BstX2I RGATCY 2 cut(s) 199, 254
BstYI RGATCY 2 cut(s) 199, 254
BsuRI GGCC 1 cut(s) 573
BtsCI GGATG 2 cut(s) 307, 518
BtsIMutI CAGTG 2 cut(s) 86, 349
BveI ACCTGC 1 cut(s) 919
Cac8I GCNNGC 1 cut(s) 270
CfoI GCGC 1 cut(s) 268
Cfr10I RCCGGY 1 cut(s) 569
Cfr13I GGNCC 3 cut(s) 572, 770, 902
CseI GACGC 2 cut(s) 14, 135
Csp6I GTAC 1 cut(s) 507
CviAII CATG 1 cut(s) 69
CviJI RGCY 5 cut(s) 15, 43, 293, 323, 573
CviKI_1 RGCY 5 cut(s) 15, 43, 293, 323, 573
CviQI GTAC 1 cut(s) 507
DdeI CTNAG 2 cut(s) 225, 417
DpnI GATC 6 cut(s) 135, 201, 219, 256, 456, 738
DpnII GATC 6 cut(s) 133, 199, 217, 254, 454, 736
Eco130I CCWWGG 4 cut(s) 554, 686, 752, 818
Eco32I GATATC 1 cut(s) 364
Eco47I GGWCC 2 cut(s) 770, 902
EcoRII CCWGG 5 cut(s) 250, 606, 672, 804, 936
EcoRV GATATC 1 cut(s) 364
EcoT14I CCWWGG 4 cut(s) 554, 686, 752, 818
EcoT22I ATGCAT 1 cut(s) 307
ErhI CCWWGG 4 cut(s) 554, 686, 752, 818
FaeI CATG 1 cut(s) 72
FalI AAGNNNNNCTT 2 cut(s) 826, 858
FaqI GGGAC 5 cut(s) 226, 572, 704, 770, 822
FatI CATG 1 cut(s) 68
FbaI TGATCA 1 cut(s) 133
FblI GTMKAC 2 cut(s) 339, 603
FokI GGATG 2 cut(s) 314, 525
FspBI CTAG 3 cut(s) 125, 555, 970
GlaI GCGC 1 cut(s) 267
GsuI CTGGAG 1 cut(s) 37
HaeII RGCGCY 1 cut(s) 269
HaeIII GGCC 1 cut(s) 573
HapII CCGG 2 cut(s) 438, 570
HgaI GACGC 2 cut(s) 14, 135
HhaI GCGC 1 cut(s) 268
Hin1II CATG 1 cut(s) 72
Hin6I GCGC 1 cut(s) 266
HinP1I GCGC 1 cut(s) 266
HinfI GANTC 2 cut(s) 239, 494
HpaII CCGG 2 cut(s) 438, 570
Hpy166II GTNNAC 2 cut(s) 340, 604
Hpy188I TCNGA 9 cut(s) 98, 217, 454, 597, 621, 663, 729, 868, 951
Hpy188III TCNNGA 2 cut(s) 438, 740
Hpy8I GTNNAC 2 cut(s) 340, 604
HpyAV CCTTC 1 cut(s) 5
HpyCH4III ACNGT 4 cut(s) 26, 208, 344, 945
HpyCH4IV ACGT 1 cut(s) 188
HpyCH4V TGCA 5 cut(s) 60, 72, 83, 305, 914
HpyF10VI GCNNNNNNNGC 1 cut(s) 117
HpyF3I CTNAG 2 cut(s) 225, 417
HpySE526I ACGT 1 cut(s) 188
Hsp92II CATG 1 cut(s) 72
HspAI GCGC 1 cut(s) 266
Kpn2I TCCGGA 1 cut(s) 437
Ksp22I TGATCA 1 cut(s) 133
Kzo9I GATC 6 cut(s) 133, 199, 217, 254, 454, 736
LmnI GCTCC 1 cut(s) 277
LweI GCATC 2 cut(s) 292, 314
MaeI CTAG 3 cut(s) 125, 555, 970
MaeII ACGT 1 cut(s) 188
MaeIII GTNAC 1 cut(s) 652
MalI GATC 6 cut(s) 135, 201, 219, 256, 456, 738
MboI GATC 6 cut(s) 133, 199, 217, 254, 454, 736
MboII GAAGA 3 cut(s) 249, 299, 415
MflI RGATCY 2 cut(s) 199, 254
MlyI GAGTC 1 cut(s) 248
MmeI TCCRAC 6 cut(s) 227, 731, 797, 839, 863, 929
MnlI CCTC 6 cut(s) 92, 285, 363, 474, 535, 865
Mph1103I ATGCAT 1 cut(s) 307
MroI TCCGGA 1 cut(s) 437
MseI TTAA 2 cut(s) 446, 927
MspI CCGG 2 cut(s) 438, 570
MspR9I CCNGG 5 cut(s) 252, 608, 674, 806, 938
Mva1269I GAATGC 1 cut(s) 171
MvaI CCWGG 5 cut(s) 252, 608, 674, 806, 938
MvnI CGCG 1 cut(s) 8
MwoI GCNNNNNNNGC 1 cut(s) 117
NdeII GATC 6 cut(s) 133, 199, 217, 254, 454, 736
NlaIII CATG 1 cut(s) 72
NsiI ATGCAT 1 cut(s) 307
PaqCI CACCTGC 1 cut(s) 919
PctI GAATGC 1 cut(s) 171
PfeI GAWTC 1 cut(s) 494
PfoI TCCNGGA 1 cut(s) 250
PleI GAGTC 1 cut(s) 247
PpsI GAGTC 1 cut(s) 247
Ppu21I YACGTR 1 cut(s) 189
Psp6I CCWGG 5 cut(s) 250, 606, 672, 804, 936
PspGI CCWGG 5 cut(s) 250, 606, 672, 804, 936
PspPI GGNCC 3 cut(s) 572, 770, 902
PstI CTGCAG 1 cut(s) 916
PsuI RGATCY 2 cut(s) 199, 254
RsaI GTAC 1 cut(s) 508
RsaNI GTAC 1 cut(s) 507
SaqAI TTAA 2 cut(s) 446, 927
Sau3AI GATC 6 cut(s) 133, 199, 217, 254, 454, 736
Sau96I GGNCC 3 cut(s) 572, 770, 902
SchI GAGTC 1 cut(s) 248
ScrFI CCNGG 5 cut(s) 252, 608, 674, 806, 938
SfaNI GCATC 2 cut(s) 292, 314
SfcI CTRYAG 5 cut(s) 582, 648, 714, 780, 912
SinI GGWCC 2 cut(s) 770, 902
SmlI CTYRAG 1 cut(s) 354
SmoI CTYRAG 1 cut(s) 354
SsiI CCGC 1 cut(s) 8
SspMI CTAG 3 cut(s) 125, 555, 970
StyD4I CCNGG 5 cut(s) 250, 606, 672, 804, 936
StyI CCWWGG 4 cut(s) 554, 686, 752, 818
TaaI ACNGT 4 cut(s) 26, 208, 344, 945
TaiI ACGT 1 cut(s) 191
TaqI TCGA 2 cut(s) 154, 237
TatI WGTACW 1 cut(s) 506
TfiI GAWTC 1 cut(s) 494
Tru1I TTAA 2 cut(s) 446, 927
Tru9I TTAA 2 cut(s) 446, 927
TscAI CASTG 2 cut(s) 93, 349
TspDTI ATGAA 1 cut(s) 201
TspGWI ACGGA 1 cut(s) 314
TspRI CASTG 2 cut(s) 93, 349
VpaK11BI GGWCC 2 cut(s) 770, 902
XmaJI CCTAGG 1 cut(s) 554
XmiI GTMKAC 2 cut(s) 339, 603
XspI CTAG 3 cut(s) 125, 555, 970
Zsp2I ATGCAT 1 cut(s) 307
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.