Rh5DG253700

B3 DNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0001196
Physical Location & Seq
Reverse (-)
29338509 .. 29356094
17586 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG253700.1

Sequence Viewer

Length: 528 bp
ATGAAGATTAATTTCAAGAGGAGCAATGCTAGTCCAGTAGTACGTTGCAGACAAGGAGTTAAGTGGGTTAAACCTAGGGTAGGACAGTTTAAGATGAACTGTGATGGAGCGTATATAGCTTCAACTAGACATGGAGAAGCTCGGTGTGTATTGCGGAACAGTGAGGGTGAGTTTTTGGCAGTTGCCACATATAAGACAGATCCAACAGTTTCATCTGATCCTACTGAGAAAGTTGTCGAGTCCCTTTCTCCTGGATCTTCACAAGCGCCTGCTCCTCATTTTGGAGAAGAAGCCAATACGGATGCATCTAAAGGAGATGCAGCTTGTAAATTCAAAGTAGGCAGTGCAACACCTCGAGTGGATGCCCCCACAGGAAACCTTTTACACCCTGACAATCTGCCGAAGATGACAAGTGCTGAGCAACTCCAAAAAAGTCTCCGGAAAGAAGGTGCTGTCTCGGTTTATGACATTGCTGAAGGAGGAGCTGGAGATTGGTTTAGAGGTGAGCTCCAGCGATCCAGACCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

175

Amino Acids

18.89

Weight (kDa)

9.1

Isoelectric Point (pI)

40.62

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 438
AciI CCGC 1 cut(s) 154
AclWI GGATC 4 cut(s) 194, 212, 262, 510
AcsI RAATTY 1 cut(s) 329
AcuI CTGAAG 1 cut(s) 495
AfaI GTAC 1 cut(s) 42
AfiI CCNNNNNNNGG 2 cut(s) 80, 281
AgsI TTSAA 3 cut(s) 16, 123, 334
AjnI CCWGG 1 cut(s) 250
AluBI AGCT 5 cut(s) 119, 140, 323, 485, 508
AluI AGCT 5 cut(s) 119, 140, 323, 485, 508
Alw21I GWGCWC 1 cut(s) 510
Alw26I GTCTC 2 cut(s) 440, 460
AlwI GGATC 4 cut(s) 194, 212, 262, 510
Ama87I CYCGRG 1 cut(s) 354
Aor13HI TCCGGA 1 cut(s) 438
ApeKI GCWGC 1 cut(s) 320
ApoI RAATTY 1 cut(s) 329
AseI ATTAAT 1 cut(s) 9
AspA2I CCTAGG 1 cut(s) 74
AspLEI GCGC 1 cut(s) 268
AsuHPI GGTGA 2 cut(s) 179, 515
AvaI CYCGRG 1 cut(s) 354
AvrII CCTAGG 1 cut(s) 74
BanII GRGCYC 1 cut(s) 510
Bbv12I GWGCWC 1 cut(s) 510
BbvI GCAGC 1 cut(s) 332
BccI CCATC 1 cut(s) 98
BciT130I CCWGG 1 cut(s) 252
BcoDI GTCTC 2 cut(s) 440, 460
BfaI CTAG 3 cut(s) 30, 75, 126
BfoI RGCGCY 1 cut(s) 269
BisI GCNGC 1 cut(s) 321
BlnI CCTAGG 1 cut(s) 74
BlpI GCTNAGC 1 cut(s) 417
BlsI GCNGC 1 cut(s) 322
Bme1390I CCNGG 1 cut(s) 252
BmeT110I CYCGRG 1 cut(s) 354
BmrFI CCNGG 1 cut(s) 252
BmsI GCATC 4 cut(s) 292, 307, 314, 352
BplI GAGNNNNNCTC 2 cut(s) 492, 524
BpmI CTGGAG 2 cut(s) 494, 507
Bpu1102I GCTNAGC 1 cut(s) 417
BsaJI CCNNGG 1 cut(s) 74
BsaWI WCCGGW 1 cut(s) 438
Bsc4I CCNNNNNNNGG 2 cut(s) 80, 281
Bse1I ACTGG 1 cut(s) 35
Bse3DI GCAATG 2 cut(s) 31, 468
BseAI TCCGGA 1 cut(s) 438
BseBI CCWGG 1 cut(s) 252
BseDI CCNNGG 1 cut(s) 74
BseGI GGATG 2 cut(s) 307, 367
BseLI CCNNNNNNNGG 2 cut(s) 80, 281
BseMI GCAATG 2 cut(s) 31, 468
BseMII CTCAG 2 cut(s) 216, 408
BseNI ACTGG 1 cut(s) 35
BseRI GAGGAG 3 cut(s) 34, 264, 495
BseXI GCAGC 1 cut(s) 332
BsiHKAI GWGCWC 1 cut(s) 510
BsiHKCI CYCGRG 1 cut(s) 354
BsiSI CCGG 1 cut(s) 439
BslFI GGGAC 1 cut(s) 226
BslI CCNNNNNNNGG 2 cut(s) 80, 281
BsmAI GTCTC 2 cut(s) 440, 460
BsmFI GGGAC 1 cut(s) 226
BsoBI CYCGRG 1 cut(s) 354
Bsp1286I GDGCHC 1 cut(s) 510
Bsp13I TCCGGA 1 cut(s) 438
Bsp143I GATC 4 cut(s) 199, 217, 254, 515
Bsp1720I GCTNAGC 1 cut(s) 417
BspACI CCGC 1 cut(s) 154
BspCNI CTCAG 2 cut(s) 217, 409
BspEI TCCGGA 1 cut(s) 438
BspPI GGATC 4 cut(s) 194, 212, 262, 510
BsrDI GCAATG 2 cut(s) 31, 468
BsrI ACTGG 1 cut(s) 35
BssECI CCNNGG 1 cut(s) 74
BssMI GATC 4 cut(s) 199, 217, 254, 515
BssT1I CCWWGG 1 cut(s) 74
Bst2UI CCWGG 1 cut(s) 252
Bst4CI ACNGT 4 cut(s) 87, 101, 161, 208
BstC8I GCNNGC 1 cut(s) 270
BstDEI CTNAG 2 cut(s) 225, 417
BstENI CCTNNNNNAGG 1 cut(s) 78
BstF5I GGATG 2 cut(s) 307, 367
BstH2I RGCGCY 1 cut(s) 269
BstHHI GCGC 1 cut(s) 268
BstKTI GATC 4 cut(s) 202, 220, 257, 518
BstMAI GTCTC 2 cut(s) 440, 460
BstMBI GATC 4 cut(s) 199, 217, 254, 515
BstMWI GCNNNNNNNGC 1 cut(s) 116
BstNI CCWGG 1 cut(s) 252
BstSCI CCNGG 1 cut(s) 250
BstV1I GCAGC 1 cut(s) 332
BstX2I RGATCY 2 cut(s) 199, 254
BstYI RGATCY 2 cut(s) 199, 254
BtsCI GGATG 2 cut(s) 307, 367
BtsI GCAGTG 1 cut(s) 349
BtsIMutI CAGTG 2 cut(s) 166, 349
Cac8I GCNNGC 1 cut(s) 270
CfoI GCGC 1 cut(s) 268
Csp6I GTAC 1 cut(s) 41
CviAII CATG 1 cut(s) 131
CviJI RGCY 6 cut(s) 119, 140, 293, 323, 485, 508
CviKI_1 RGCY 6 cut(s) 119, 140, 293, 323, 485, 508
CviQI GTAC 1 cut(s) 41
DdeI CTNAG 2 cut(s) 225, 417
DpnI GATC 4 cut(s) 201, 219, 256, 517
DpnII GATC 4 cut(s) 199, 217, 254, 515
Ecl136II GAGCTC 1 cut(s) 508
Eco130I CCWWGG 1 cut(s) 74
Eco24I GRGCYC 1 cut(s) 510
Eco53kI GAGCTC 1 cut(s) 508
Eco57I CTGAAG 1 cut(s) 495
Eco88I CYCGRG 1 cut(s) 354
EcoICRI GAGCTC 1 cut(s) 508
EcoNI CCTNNNNNAGG 1 cut(s) 78
EcoRII CCWGG 1 cut(s) 250
EcoT14I CCWWGG 1 cut(s) 74
EcoT22I ATGCAT 1 cut(s) 307
EcoT38I GRGCYC 1 cut(s) 510
ErhI CCWWGG 1 cut(s) 74
FaeI CATG 1 cut(s) 134
FaiI YATR 7 cut(s) 114, 116, 132, 190, 192, 465, 526
FaqI GGGAC 1 cut(s) 226
FatI CATG 1 cut(s) 130
Fnu4HI GCNGC 1 cut(s) 321
FokI GGATG 2 cut(s) 314, 374
FriOI GRGCYC 1 cut(s) 510
Fsp4HI GCNGC 1 cut(s) 321
FspBI CTAG 3 cut(s) 30, 75, 126
GlaI GCGC 1 cut(s) 267
GluI GCNGC 1 cut(s) 321
GsuI CTGGAG 2 cut(s) 494, 507
HaeII RGCGCY 1 cut(s) 269
HapII CCGG 1 cut(s) 439
HhaI GCGC 1 cut(s) 268
Hin1II CATG 1 cut(s) 134
Hin6I GCGC 1 cut(s) 266
HinP1I GCGC 1 cut(s) 266
HinfI GANTC 1 cut(s) 239
HpaII CCGG 1 cut(s) 439
HphI GGTGA 2 cut(s) 179, 515
Hpy188I TCNGA 1 cut(s) 217
Hpy188III TCNNGA 3 cut(s) 16, 439, 519
HpyAV CCTTC 2 cut(s) 440, 470
HpyCH4III ACNGT 4 cut(s) 87, 101, 161, 208
HpyCH4IV ACGT 1 cut(s) 43
HpyCH4V TGCA 4 cut(s) 48, 305, 320, 347
HpyF10VI GCNNNNNNNGC 1 cut(s) 116
HpyF3I CTNAG 2 cut(s) 225, 417
HpySE526I ACGT 1 cut(s) 43
Hsp92II CATG 1 cut(s) 134
HspAI GCGC 1 cut(s) 266
Kpn2I TCCGGA 1 cut(s) 438
Kzo9I GATC 4 cut(s) 199, 217, 254, 515
LmnI GCTCC 5 cut(s) 21, 107, 277, 482, 513
LpnPI CCDG 9 cut(s) 48, 237, 264, 282, 357, 402, 452, 471, 524
Lsp1109I GCAGC 1 cut(s) 332
LweI GCATC 4 cut(s) 292, 307, 314, 352
MaeI CTAG 3 cut(s) 30, 75, 126
MaeII ACGT 1 cut(s) 43
MalI GATC 4 cut(s) 201, 219, 256, 517
MboI GATC 4 cut(s) 199, 217, 254, 515
MboII GAAGA 4 cut(s) 16, 249, 299, 415
MflI RGATCY 2 cut(s) 199, 254
MhlI GDGCHC 1 cut(s) 510
MluCI AATT 2 cut(s) 10, 329
MlyI GAGTC 1 cut(s) 248
MmeI TCCRAC 1 cut(s) 227
MnlI CCTC 6 cut(s) 12, 157, 285, 363, 473, 494
Mph1103I ATGCAT 1 cut(s) 307
MroI TCCGGA 1 cut(s) 438
MseI TTAA 4 cut(s) 9, 60, 69, 90
MspI CCGG 1 cut(s) 439
MspR9I CCNGG 1 cut(s) 252
MvaI CCWGG 1 cut(s) 252
MwoI GCNNNNNNNGC 1 cut(s) 116
NdeII GATC 4 cut(s) 199, 217, 254, 515
NlaIII CATG 1 cut(s) 134
NsiI ATGCAT 1 cut(s) 307
PaeR7I CTCGAG 1 cut(s) 354
PfoI TCCNGGA 1 cut(s) 250
PkrI GCNGC 1 cut(s) 322
PleI GAGTC 1 cut(s) 247
PpsI GAGTC 1 cut(s) 247
PshBI ATTAAT 1 cut(s) 9
Psp124BI GAGCTC 1 cut(s) 510
Psp6I CCWGG 1 cut(s) 250
PspGI CCWGG 1 cut(s) 250
PspXI VCTCGAGB 1 cut(s) 354
PsuI RGATCY 2 cut(s) 199, 254
RsaI GTAC 1 cut(s) 42
RsaNI GTAC 1 cut(s) 41
SacI GAGCTC 1 cut(s) 510
SaqAI TTAA 4 cut(s) 9, 60, 69, 90
SatI GCNGC 1 cut(s) 321
Sau3AI GATC 4 cut(s) 199, 217, 254, 515
SchI GAGTC 1 cut(s) 248
ScrFI CCNGG 1 cut(s) 252
SduI GDGCHC 1 cut(s) 510
SfaNI GCATC 4 cut(s) 292, 307, 314, 352
Sfr274I CTCGAG 1 cut(s) 354
SlaI CTCGAG 1 cut(s) 354
SmlI CTYRAG 1 cut(s) 354
SmoI CTYRAG 1 cut(s) 354
Sse9I AATT 2 cut(s) 10, 329
SsiI CCGC 1 cut(s) 154
SspMI CTAG 3 cut(s) 30, 75, 126
SstI GAGCTC 1 cut(s) 510
StyD4I CCNGG 1 cut(s) 250
StyI CCWWGG 1 cut(s) 74
TaaI ACNGT 4 cut(s) 87, 101, 161, 208
TaiI ACGT 1 cut(s) 46
TaqI TCGA 2 cut(s) 237, 355
TasI AATT 2 cut(s) 10, 329
Tru1I TTAA 4 cut(s) 9, 60, 69, 90
Tru9I TTAA 4 cut(s) 9, 60, 69, 90
TscAI CASTG 2 cut(s) 166, 349
TseI GCWGC 1 cut(s) 320
TspDTI ATGAA 3 cut(s) 17, 110, 201
TspGWI ACGGA 1 cut(s) 314
TspRI CASTG 2 cut(s) 166, 349
VspI ATTAAT 1 cut(s) 9
XagI CCTNNNNNAGG 1 cut(s) 78
XapI RAATTY 1 cut(s) 329
XhoI CTCGAG 1 cut(s) 354
XmaJI CCTAGG 1 cut(s) 74
XspI CTAG 3 cut(s) 30, 75, 126
Zsp2I ATGCAT 1 cut(s) 307
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.