Rh5DG254800

B3 DNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0001196
Physical Location & Seq
Reverse (-)
29832952 .. 29841052
8101 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG254800.1

Sequence Viewer

Length: 1257 bp
ATGGCTGCCAAAGCTTCGTGCTTTTTCTGCAAGGCTGACAGTGGTCAGTTCGTTAATGGCTGGGAGCTCAAGGATGGGTCTTTCGCCCAGCTCTGCAAGAAATGCGGTTTTGCTTATGTGAATGGTATATTTTGTGAAACTTTTCACATGGAAGTAGAAGGCTGGAGAGACTGTGCTGTTTGTGGGAAGCCACTCCATTGTGGGTGCATTATGTCACGCCACAAGTATGCACCACTGGATTTCGGAGGTGTAGGTTGCACAAAATGCTTACTAGAAGTTGATCAATCTATTGCTTCAGGTCGAATGACCAGAATGCTGAAAGAAATAGTTGCCAAGTACAAGACGGACCCAACAGTTTCATCTGATCCTACTAAGAAAGTTTTTGAGTCCCTTTTTCCTGAATCTTTACAAGCTCCAGCACCTCAGATTGGAGAAGAATCCAATACGGATGCATCTAAAGGAGATACAGCTTGTGAAGCCAAAGTAGGCACTGAAACACCTCAAGTGGATGCCTCCACAGGAAACCATTTACACCCTGACAATCTGCCGAAGATGACAAGGGCTGAGCAACTCCAAAAAGTCTCCAGAAACTTAAACTCTGATCTTATACCTCTGTTTGAGAAGTCATCAACCGTAAGTGATACTAATCACGGTACAGGATTTCTTGTGATACCGAAGAAATATGCAGGGGAACAATCTCCAAGGGACATTGAAACAGGTCTAACACCTGTAGATACAAGTCTGAAAGCAGATCAGGAACAATCTCCAATGGATGTGGTAACAAATCCAATGCCTATAGTAACAAATCAGAAAACAAACCAGGAACAATCTCCAAGGGACAATGAAACAGGTCCAACGCGTGTAGATACAACTCTGAAACCAGACCAGGAACAATCTCCGATGGTTATGGAAACAAGTCCAGTGCCTATAGAAACAAATCAGAAAACGAACCAGGAACAATCTCTAAGGGACTTTGAAACAGGTCCAACGCCTGTAGATACAAGTCTGAAAACAGACCAGGAACAACCTCCAAAGGATATGGAGACAAGTCCAATGCCTGTAGAAACAAGTCATAAATTAGACCAGGGACAATCTCCAAAGGATATGGAAACAGGTCCAACGCCTGTAGAAACAAGTCTGAAAACAGACCAGGACTGTGAACCCTGTGCTATCCTTGGATGGGTGAAGGCCTTTTATGCAATTAGAGTTTCATCTCCATTAGGGTTTTCAAACTTTCTGCGCCGAAGTGGAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

418

Amino Acids

45.81

Weight (kDa)

5.04

Isoelectric Point (pI)

48.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf_VAL1_N PF25813 47 - 91 6.1e-21 VAL1-like, N-terminal zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 859
AciI CCGC 1 cut(s) 105
AclWI GGATC 1 cut(s) 359
AcuI CTGAAG 1 cut(s) 279
AfaI GTAC 2 cut(s) 338, 655
AfiI CCNNNNNNNGG 2 cut(s) 428, 1180
AflIII ACRYGT 1 cut(s) 857
AgsI TTSAA 3 cut(s) 713, 977, 1230
AjnI CCWGG 6 cut(s) 819, 885, 951, 1017, 1083, 1149
AluBI AGCT 5 cut(s) 14, 67, 91, 413, 470
AluI AGCT 5 cut(s) 14, 67, 91, 413, 470
Alw21I GWGCWC 1 cut(s) 69
Alw26I GTCTC 3 cut(s) 162, 586, 1037
AlwI GGATC 1 cut(s) 359
AoxI GGCC 1 cut(s) 1188
ApeKI GCWGC 1 cut(s) 5
Asp700I GAANNNNTTC 1 cut(s) 141
AspLEI GCGC 1 cut(s) 1242
AspS9I GGNCC 4 cut(s) 346, 851, 983, 1115
AsuHPI GGTGA 1 cut(s) 1195
AvaII GGWCC 4 cut(s) 346, 851, 983, 1115
BanII GRGCYC 1 cut(s) 69
Bbv12I GWGCWC 1 cut(s) 69
BccI CCATC 3 cut(s) 68, 895, 1173
BciT130I CCWGG 6 cut(s) 821, 887, 953, 1019, 1085, 1151
BclI TGATCA 1 cut(s) 280
BcoDI GTCTC 3 cut(s) 162, 586, 1037
BfaI CTAG 1 cut(s) 272
BfmI CTRYAG 6 cut(s) 729, 795, 927, 993, 1059, 1125
BisI GCNGC 1 cut(s) 6
BlpI GCTNAGC 1 cut(s) 564
BlsI GCNGC 1 cut(s) 7
Bme1390I CCNGG 6 cut(s) 821, 887, 953, 1019, 1085, 1151
Bme18I GGWCC 4 cut(s) 346, 851, 983, 1115
BmgT120I GGNCC 4 cut(s) 346, 851, 983, 1115
BmiI GGNNCC 1 cut(s) 348
BmrFI CCNGG 6 cut(s) 821, 887, 953, 1019, 1085, 1151
BmsI GCATC 3 cut(s) 439, 461, 499
BoxI GACNNNNGTC 1 cut(s) 42
BpmI CTGGAG 3 cut(s) 184, 399, 568
Bpu1102I GCTNAGC 1 cut(s) 564
BpuEI CTTGAG 2 cut(s) 53, 486
BsaBI GATNNNNATC 1 cut(s) 645
BsaJI CCNNGG 4 cut(s) 701, 833, 1084, 1174
BsaXI ACNNNNNCTCC 2 cut(s) 157, 187
Bsc4I CCNNNNNNNGG 2 cut(s) 428, 1180
Bse1I ACTGG 2 cut(s) 240, 920
Bse8I GATNNNNATC 1 cut(s) 645
BseBI CCWGG 6 cut(s) 821, 887, 953, 1019, 1085, 1151
BseDI CCNNGG 4 cut(s) 701, 833, 1084, 1174
BseGI GGATG 5 cut(s) 79, 454, 514, 778, 1184
BseJI GATNNNNATC 1 cut(s) 645
BseLI CCNNNNNNNGG 2 cut(s) 428, 1180
BseMII CTCAG 2 cut(s) 437, 555
BseNI ACTGG 2 cut(s) 240, 920
BseYI CCCAGC 2 cut(s) 60, 87
Bsh1236I CGCG 1 cut(s) 859
BshFI GGCC 1 cut(s) 1190
BsiHKAI GWGCWC 1 cut(s) 69
BslFI GGGAC 5 cut(s) 373, 719, 851, 983, 1101
BslI CCNNNNNNNGG 2 cut(s) 428, 1180
BsmAI GTCTC 3 cut(s) 162, 586, 1037
BsmFI GGGAC 5 cut(s) 373, 719, 851, 983, 1101
BsmI GAATGC 1 cut(s) 318
BsnI GGCC 1 cut(s) 1190
Bsp1286I GDGCHC 1 cut(s) 69
Bsp143I GATC 4 cut(s) 280, 364, 601, 751
Bsp1720I GCTNAGC 1 cut(s) 564
BspACI CCGC 1 cut(s) 105
BspANI GGCC 1 cut(s) 1190
BspCNI CTCAG 2 cut(s) 436, 556
BspFNI CGCG 1 cut(s) 859
BspLI GGNNCC 1 cut(s) 348
BspPI GGATC 1 cut(s) 359
BsrI ACTGG 2 cut(s) 240, 920
BssECI CCNNGG 4 cut(s) 701, 833, 1084, 1174
BssMI GATC 4 cut(s) 280, 364, 601, 751
BssT1I CCWWGG 3 cut(s) 701, 833, 1174
Bst2UI CCWGG 6 cut(s) 821, 887, 953, 1019, 1085, 1151
Bst4CI ACNGT 6 cut(s) 41, 173, 355, 634, 653, 1157
BstAPI GCANNNNNTGC 2 cut(s) 102, 264
BstDEI CTNAG 4 cut(s) 372, 423, 564, 965
BstF5I GGATG 5 cut(s) 79, 454, 514, 778, 1184
BstFNI CGCG 1 cut(s) 859
BstHHI GCGC 1 cut(s) 1242
BstKTI GATC 4 cut(s) 283, 367, 604, 754
BstMAI GTCTC 3 cut(s) 162, 586, 1037
BstMBI GATC 4 cut(s) 280, 364, 601, 751
BstMWI GCNNNNNNNGC 6 cut(s) 11, 27, 102, 264, 476, 1196
BstNI CCWGG 6 cut(s) 821, 887, 953, 1019, 1085, 1151
BstPAI GACNNNNGTC 1 cut(s) 42
BstSCI CCNGG 6 cut(s) 819, 885, 951, 1017, 1083, 1149
BstSFI CTRYAG 6 cut(s) 729, 795, 927, 993, 1059, 1125
BstUI CGCG 1 cut(s) 859
BsuRI GGCC 1 cut(s) 1190
BtsCI GGATG 5 cut(s) 79, 454, 514, 778, 1184
BtsIMutI CAGTG 4 cut(s) 46, 233, 489, 927
CfoI GCGC 1 cut(s) 1242
Cfr13I GGNCC 4 cut(s) 346, 851, 983, 1115
Csp6I GTAC 2 cut(s) 337, 654
CspCI CAANNNNNGTGG 2 cut(s) 756, 791
CviAII CATG 1 cut(s) 148
CviQI GTAC 2 cut(s) 337, 654
DdeI CTNAG 4 cut(s) 372, 423, 564, 965
DpnI GATC 4 cut(s) 282, 366, 603, 753
DpnII GATC 4 cut(s) 280, 364, 601, 751
Ecl136II GAGCTC 1 cut(s) 67
Eco130I CCWWGG 3 cut(s) 701, 833, 1174
Eco147I AGGCCT 1 cut(s) 1190
Eco24I GRGCYC 1 cut(s) 69
Eco47I GGWCC 4 cut(s) 346, 851, 983, 1115
Eco53kI GAGCTC 1 cut(s) 67
Eco57I CTGAAG 1 cut(s) 279
EcoICRI GAGCTC 1 cut(s) 67
EcoRII CCWGG 6 cut(s) 819, 885, 951, 1017, 1083, 1149
EcoT14I CCWWGG 3 cut(s) 701, 833, 1174
EcoT22I ATGCAT 1 cut(s) 454
EcoT38I GRGCYC 1 cut(s) 69
ErhI CCWWGG 3 cut(s) 701, 833, 1174
FaeI CATG 1 cut(s) 151
FaqI GGGAC 5 cut(s) 373, 719, 851, 983, 1101
FatI CATG 1 cut(s) 147
FbaI TGATCA 1 cut(s) 280
Fnu4HI GCNGC 1 cut(s) 6
FokI GGATG 5 cut(s) 86, 461, 521, 785, 1191
FriOI GRGCYC 1 cut(s) 69
Fsp4HI GCNGC 1 cut(s) 6
FspBI CTAG 1 cut(s) 272
GlaI GCGC 1 cut(s) 1241
GluI GCNGC 1 cut(s) 6
GsaI CCCAGC 2 cut(s) 64, 91
GsuI CTGGAG 3 cut(s) 184, 399, 568
HaeIII GGCC 1 cut(s) 1190
HhaI GCGC 1 cut(s) 1242
Hin1II CATG 1 cut(s) 151
Hin6I GCGC 1 cut(s) 1240
HinP1I GCGC 1 cut(s) 1240
HindIII AAGCTT 1 cut(s) 12
HinfI GANTC 3 cut(s) 386, 401, 437
HphI GGTGA 1 cut(s) 1195
Hpy166II GTNNAC 1 cut(s) 1160
Hpy188III TCNNGA 3 cut(s) 398, 585, 755
Hpy8I GTNNAC 1 cut(s) 1160
HpyAV CCTTC 2 cut(s) 152, 1180
HpyCH4III ACNGT 6 cut(s) 41, 173, 355, 634, 653, 1157
HpyCH4V TGCA 8 cut(s) 30, 96, 207, 230, 258, 452, 686, 1199
HpyF10VI GCNNNNNNNGC 6 cut(s) 11, 27, 102, 264, 476, 1196
HpyF3I CTNAG 4 cut(s) 372, 423, 564, 965
Hsp92II CATG 1 cut(s) 151
HspAI GCGC 1 cut(s) 1240
Ksp22I TGATCA 1 cut(s) 280
Kzo9I GATC 4 cut(s) 280, 364, 601, 751
LmnI GCTCC 2 cut(s) 64, 418
LweI GCATC 3 cut(s) 439, 461, 499
MaeI CTAG 1 cut(s) 272
MaeIII GTNAC 3 cut(s) 213, 778, 799
MalI GATC 4 cut(s) 282, 366, 603, 753
MboI GATC 4 cut(s) 280, 364, 601, 751
MboII GAAGA 3 cut(s) 446, 562, 688
MhlI GDGCHC 1 cut(s) 69
MluCI AATT 3 cut(s) 1076, 1200, 1252
MluI ACGCGT 1 cut(s) 857
MlyI GAGTC 1 cut(s) 395
MmeI TCCRAC 3 cut(s) 878, 1010, 1142
MnlI CCTC 6 cut(s) 239, 432, 510, 523, 621, 1038
Mph1103I ATGCAT 1 cut(s) 454
MroXI GAANNNNTTC 1 cut(s) 141
MseI TTAA 2 cut(s) 54, 593
MslI CAYNNNNRTG 1 cut(s) 225
MspR9I CCNGG 6 cut(s) 821, 887, 953, 1019, 1085, 1151
Mva1269I GAATGC 1 cut(s) 318
MvaI CCWGG 6 cut(s) 821, 887, 953, 1019, 1085, 1151
MvnI CGCG 1 cut(s) 859
MwoI GCNNNNNNNGC 6 cut(s) 11, 27, 102, 264, 476, 1196
NdeII GATC 4 cut(s) 280, 364, 601, 751
NlaIII CATG 1 cut(s) 151
NlaIV GGNNCC 1 cut(s) 348
NmuCI GTSAC 1 cut(s) 213
NsiI ATGCAT 1 cut(s) 454
PceI AGGCCT 1 cut(s) 1190
PctI GAATGC 1 cut(s) 318
PdmI GAANNNNTTC 1 cut(s) 141
PfeI GAWTC 2 cut(s) 401, 437
PkrI GCNGC 1 cut(s) 7
PleI GAGTC 1 cut(s) 394
PpsI GAGTC 1 cut(s) 394
PshAI GACNNNNGTC 1 cut(s) 42
Psp124BI GAGCTC 1 cut(s) 69
Psp6I CCWGG 6 cut(s) 819, 885, 951, 1017, 1083, 1149
PspFI CCCAGC 2 cut(s) 60, 87
PspGI CCWGG 6 cut(s) 819, 885, 951, 1017, 1083, 1149
PspN4I GGNNCC 1 cut(s) 348
PspPI GGNCC 4 cut(s) 346, 851, 983, 1115
RsaI GTAC 2 cut(s) 338, 655
RsaNI GTAC 2 cut(s) 337, 654
RseI CAYNNNNRTG 1 cut(s) 225
SacI GAGCTC 1 cut(s) 69
SaqAI TTAA 2 cut(s) 54, 593
SatI GCNGC 1 cut(s) 6
Sau3AI GATC 4 cut(s) 280, 364, 601, 751
Sau96I GGNCC 4 cut(s) 346, 851, 983, 1115
SchI GAGTC 1 cut(s) 395
ScrFI CCNGG 6 cut(s) 821, 887, 953, 1019, 1085, 1151
SduI GDGCHC 1 cut(s) 69
SfaNI GCATC 3 cut(s) 439, 461, 499
SfcI CTRYAG 6 cut(s) 729, 795, 927, 993, 1059, 1125
SinI GGWCC 4 cut(s) 346, 851, 983, 1115
SmiMI CAYNNNNRTG 1 cut(s) 225
SmlI CTYRAG 2 cut(s) 68, 501
SmoI CTYRAG 2 cut(s) 68, 501
Sse9I AATT 3 cut(s) 1076, 1200, 1252
SseBI AGGCCT 1 cut(s) 1190
SsiI CCGC 1 cut(s) 105
SspMI CTAG 1 cut(s) 272
SstI GAGCTC 1 cut(s) 69
StuI AGGCCT 1 cut(s) 1190
StyD4I CCNGG 6 cut(s) 819, 885, 951, 1017, 1083, 1149
StyI CCWWGG 3 cut(s) 701, 833, 1174
TaaI ACNGT 6 cut(s) 41, 173, 355, 634, 653, 1157
TaqI TCGA 1 cut(s) 301
TasI AATT 3 cut(s) 1076, 1200, 1252
TatI WGTACW 1 cut(s) 336
TfiI GAWTC 2 cut(s) 401, 437
Tru1I TTAA 2 cut(s) 54, 593
Tru9I TTAA 2 cut(s) 54, 593
TscAI CASTG 4 cut(s) 46, 240, 496, 927
TseFI GTSAC 1 cut(s) 213
TseI GCWGC 1 cut(s) 5
Tsp45I GTSAC 1 cut(s) 213
TspDTI ATGAA 3 cut(s) 348, 858, 1200
TspGWI ACGGA 2 cut(s) 359, 461
TspRI CASTG 4 cut(s) 46, 240, 496, 927
VpaK11BI GGWCC 4 cut(s) 346, 851, 983, 1115
XmnI GAANNNNTTC 1 cut(s) 141
XspI CTAG 1 cut(s) 272
Zsp2I ATGCAT 1 cut(s) 454
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.