Rh5AG245800

B3 DNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0001196
Physical Location & Seq
Reverse (-)
30385565 .. 30393758
8194 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG245800.1

Sequence Viewer

Length: 1173 bp
ATGGCTGCCAAAGCTTCGTGCTTTTTCTGCAAGGCCGACAGTGGTCAGTTCGTTAATGGCTGGGAGCTCAAGGATGGGTCTTTCGCCCAGCTCTGCAAGAAATGCGGTTTTGCTTATGTGAATGGTATATTTTGTGAAACTTTTCACATGGAAGCGGAAGGCTGGAGAGACTGTGCTGTTTGTGGGAAGCCACTCCATTGTGGGTGCATTATGTCACGCCACAAGTATGCACCACTGGATTTTGGAGGTGTAGGTTGCACAAAATGCTTACTAGAAGTTGATCAATCTATTGCTTCAGGTCGAATGACCAGAATGCTGAAGGAAATAGTTGCCAAGTACAAGACAGACCCAACAGTTTCATCTGATCCTACTAAGAAAGTTGTCGAGTCCCTTTTTCCTAAATCTTTACAAGCTCCAGCACCTCAGATTGGAGAAGAATCCAATACGGATGCATCTAAAGGAGATACAGCTTGTGAATCCAAAGTGGGCAGTGAAACACTTCAAGTGGATGCCTCCACAGGAAACCATTTACACCCTGACAATCTGCTGAAGATGACAAGGGCTGAGCAACTCCAAAAAGTCTCCGGAAACTTAAACTCTGATCTTATACCTCTGTTTGAGAAGTCATCGACCGTAAGTGATAGTAATCACAGTACAGGATTTCTTGTGATACCAAAGAAATATGCAGAGGAACAATCTCCAAGGGACATTGAAACAGGTCCAACACCTGTAGATACAAGTCTGAAAGCAGACCAGGAACAATCTCGAATGGATGTGGTAACAAATCCAATGCCTATAGAAACAAATCAGAAAACAAACCAAGAACAATCTCCAGGGGACATTGAAACAGGTCCAACGCGTGTATATACAACCCTGAAACCAGACCAGGAACAATCTCCGATGGTTATGGCAACAAGTCCAATGCCTATAGAAACAAATCAGAAAACGAACCAGGAACAATCTCTAAGGGACTTTGAAACAGGTCCAACGCCTGTAAATACAAGTCTGAAAACAGACGAGGAACAACCTCCAAAGGATATGGAGACAAGTCCAATGCCTGTAGAAACAAGTCATAAATCAGACCAGGGACAATCTCCAAAGGATATGGAAACAGGTCCAACGCCTGTAGAAACAAGTCTGAAAACAGACCAGGTCCAACACCTGAAGAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

390

Amino Acids

42.71

Weight (kDa)

5.14

Isoelectric Point (pI)

49.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf_VAL1_N PF25813 47 - 91 2e-21 VAL1-like, N-terminal zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 859
AccIII TCCGGA 1 cut(s) 584
AciI CCGC 2 cut(s) 105, 155
AclWI GGATC 1 cut(s) 359
AcuI CTGAAG 3 cut(s) 279, 338, 569
AfaI GTAC 2 cut(s) 338, 655
AfiI CCNNNNNNNGG 1 cut(s) 428
AflIII ACRYGT 1 cut(s) 857
AgsI TTSAA 4 cut(s) 503, 713, 845, 977
AjnI CCWGG 6 cut(s) 753, 832, 885, 951, 1083, 1149
AluBI AGCT 5 cut(s) 14, 67, 91, 413, 470
AluI AGCT 5 cut(s) 14, 67, 91, 413, 470
Alw21I GWGCWC 1 cut(s) 69
Alw26I GTCTC 3 cut(s) 162, 586, 1037
AlwI GGATC 1 cut(s) 359
Aor13HI TCCGGA 1 cut(s) 584
AoxI GGCC 1 cut(s) 33
ApeKI GCWGC 1 cut(s) 5
Asp700I GAANNNNTTC 2 cut(s) 141, 498
AspS9I GGNCC 5 cut(s) 719, 851, 983, 1115, 1153
AvaII GGWCC 5 cut(s) 719, 851, 983, 1115, 1153
BanII GRGCYC 1 cut(s) 69
Bbv12I GWGCWC 1 cut(s) 69
BccI CCATC 2 cut(s) 68, 895
BciT130I CCWGG 6 cut(s) 755, 834, 887, 953, 1085, 1151
BclI TGATCA 1 cut(s) 280
BcoDI GTCTC 3 cut(s) 162, 586, 1037
BfaI CTAG 1 cut(s) 272
BfmI CTRYAG 5 cut(s) 729, 795, 927, 1059, 1125
BisI GCNGC 1 cut(s) 6
BlpI GCTNAGC 1 cut(s) 564
BlsI GCNGC 1 cut(s) 7
Bme1390I CCNGG 6 cut(s) 755, 834, 887, 953, 1085, 1151
Bme18I GGWCC 5 cut(s) 719, 851, 983, 1115, 1153
BmgT120I GGNCC 5 cut(s) 719, 851, 983, 1115, 1153
BmrFI CCNGG 6 cut(s) 755, 834, 887, 953, 1085, 1151
BmsI GCATC 3 cut(s) 439, 461, 499
BoxI GACNNNNGTC 1 cut(s) 42
BpmI CTGGAG 3 cut(s) 184, 399, 816
Bpu1102I GCTNAGC 1 cut(s) 564
BpuEI CTTGAG 1 cut(s) 53
BsaBI GATNNNNATC 1 cut(s) 645
BsaJI CCNNGG 3 cut(s) 701, 833, 1084
BsaWI WCCGGW 1 cut(s) 584
BsaXI ACNNNNNCTCC 2 cut(s) 157, 187
Bsc4I CCNNNNNNNGG 1 cut(s) 428
Bse1I ACTGG 1 cut(s) 240
Bse8I GATNNNNATC 1 cut(s) 645
BseAI TCCGGA 1 cut(s) 584
BseBI CCWGG 6 cut(s) 755, 834, 887, 953, 1085, 1151
BseDI CCNNGG 3 cut(s) 701, 833, 1084
BseGI GGATG 4 cut(s) 79, 454, 514, 778
BseJI GATNNNNATC 1 cut(s) 645
BseLI CCNNNNNNNGG 1 cut(s) 428
BseMII CTCAG 2 cut(s) 437, 555
BseNI ACTGG 1 cut(s) 240
BseYI CCCAGC 2 cut(s) 60, 87
Bsh1236I CGCG 1 cut(s) 859
Bsh1285I CGRYCG 1 cut(s) 633
BshFI GGCC 1 cut(s) 35
BsiEI CGRYCG 1 cut(s) 633
BsiHKAI GWGCWC 1 cut(s) 69
BsiSI CCGG 1 cut(s) 585
BslFI GGGAC 5 cut(s) 373, 719, 851, 983, 1101
BslI CCNNNNNNNGG 1 cut(s) 428
BsmAI GTCTC 3 cut(s) 162, 586, 1037
BsmFI GGGAC 5 cut(s) 373, 719, 851, 983, 1101
BsmI GAATGC 1 cut(s) 318
BsnI GGCC 1 cut(s) 35
Bsp1286I GDGCHC 1 cut(s) 69
Bsp13I TCCGGA 1 cut(s) 584
Bsp143I GATC 3 cut(s) 280, 364, 601
Bsp1720I GCTNAGC 1 cut(s) 564
BspACI CCGC 2 cut(s) 105, 155
BspANI GGCC 1 cut(s) 35
BspCNI CTCAG 2 cut(s) 436, 556
BspEI TCCGGA 1 cut(s) 584
BspFNI CGCG 1 cut(s) 859
BspPI GGATC 1 cut(s) 359
BsrI ACTGG 1 cut(s) 240
BssECI CCNNGG 3 cut(s) 701, 833, 1084
BssMI GATC 3 cut(s) 280, 364, 601
BssT1I CCWWGG 1 cut(s) 701
Bst2UI CCWGG 6 cut(s) 755, 834, 887, 953, 1085, 1151
Bst4CI ACNGT 5 cut(s) 41, 173, 355, 634, 653
BstAPI GCANNNNNTGC 2 cut(s) 102, 264
BstDEI CTNAG 4 cut(s) 372, 423, 564, 965
BstF5I GGATG 4 cut(s) 79, 454, 514, 778
BstFNI CGCG 1 cut(s) 859
BstKTI GATC 3 cut(s) 283, 367, 604
BstMAI GTCTC 3 cut(s) 162, 586, 1037
BstMBI GATC 3 cut(s) 280, 364, 601
BstMCI CGRYCG 1 cut(s) 633
BstMWI GCNNNNNNNGC 4 cut(s) 11, 27, 102, 264
BstNI CCWGG 6 cut(s) 755, 834, 887, 953, 1085, 1151
BstPAI GACNNNNGTC 1 cut(s) 42
BstSCI CCNGG 6 cut(s) 753, 832, 885, 951, 1083, 1149
BstSFI CTRYAG 5 cut(s) 729, 795, 927, 1059, 1125
BstUI CGCG 1 cut(s) 859
BsuRI GGCC 1 cut(s) 35
BtsCI GGATG 4 cut(s) 79, 454, 514, 778
BtsI GCAGTG 1 cut(s) 496
BtsIMutI CAGTG 3 cut(s) 46, 233, 496
Cfr13I GGNCC 5 cut(s) 719, 851, 983, 1115, 1153
CsiI ACCWGGT 1 cut(s) 1149
Csp6I GTAC 2 cut(s) 337, 654
CviAII CATG 1 cut(s) 148
CviQI GTAC 2 cut(s) 337, 654
DdeI CTNAG 4 cut(s) 372, 423, 564, 965
DpnI GATC 3 cut(s) 282, 366, 603
DpnII GATC 3 cut(s) 280, 364, 601
Ecl136II GAGCTC 1 cut(s) 67
Eco130I CCWWGG 1 cut(s) 701
Eco24I GRGCYC 1 cut(s) 69
Eco47I GGWCC 5 cut(s) 719, 851, 983, 1115, 1153
Eco53kI GAGCTC 1 cut(s) 67
Eco57I CTGAAG 3 cut(s) 279, 338, 569
EcoICRI GAGCTC 1 cut(s) 67
EcoRII CCWGG 6 cut(s) 753, 832, 885, 951, 1083, 1149
EcoT14I CCWWGG 1 cut(s) 701
EcoT22I ATGCAT 1 cut(s) 454
EcoT38I GRGCYC 1 cut(s) 69
ErhI CCWWGG 1 cut(s) 701
FaeI CATG 1 cut(s) 151
FaqI GGGAC 5 cut(s) 373, 719, 851, 983, 1101
FatI CATG 1 cut(s) 147
FbaI TGATCA 1 cut(s) 280
Fnu4HI GCNGC 1 cut(s) 6
FokI GGATG 4 cut(s) 86, 461, 521, 785
FriOI GRGCYC 1 cut(s) 69
Fsp4HI GCNGC 1 cut(s) 6
FspBI CTAG 1 cut(s) 272
GluI GCNGC 1 cut(s) 6
GsaI CCCAGC 2 cut(s) 64, 91
GsuI CTGGAG 3 cut(s) 184, 399, 816
HaeIII GGCC 1 cut(s) 35
HapII CCGG 1 cut(s) 585
Hin1II CATG 1 cut(s) 151
HindIII AAGCTT 1 cut(s) 12
HinfI GANTC 3 cut(s) 386, 437, 476
HpaII CCGG 1 cut(s) 585
Hpy188III TCNNGA 2 cut(s) 585, 765
HpyAV CCTTC 2 cut(s) 152, 313
HpyCH4III ACNGT 5 cut(s) 41, 173, 355, 634, 653
HpyCH4V TGCA 7 cut(s) 30, 96, 207, 230, 258, 452, 686
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 27, 102, 264
HpyF3I CTNAG 4 cut(s) 372, 423, 564, 965
Hsp92II CATG 1 cut(s) 151
Kpn2I TCCGGA 1 cut(s) 584
Ksp22I TGATCA 1 cut(s) 280
Kzo9I GATC 3 cut(s) 280, 364, 601
LmnI GCTCC 2 cut(s) 64, 418
LweI GCATC 3 cut(s) 439, 461, 499
MabI ACCWGGT 1 cut(s) 1149
MaeI CTAG 1 cut(s) 272
MaeIII GTNAC 2 cut(s) 213, 778
MalI GATC 3 cut(s) 282, 366, 603
MboI GATC 3 cut(s) 280, 364, 601
MboII GAAGA 2 cut(s) 446, 562
MhlI GDGCHC 1 cut(s) 69
MluI ACGCGT 1 cut(s) 857
MlyI GAGTC 1 cut(s) 395
MmeI TCCRAC 4 cut(s) 746, 878, 1010, 1142
MnlI CCTC 7 cut(s) 239, 432, 523, 621, 682, 1012, 1038
Mph1103I ATGCAT 1 cut(s) 454
MroI TCCGGA 1 cut(s) 584
MroXI GAANNNNTTC 2 cut(s) 141, 498
MseI TTAA 2 cut(s) 54, 593
MslI CAYNNNNRTG 1 cut(s) 225
MspI CCGG 1 cut(s) 585
MspR9I CCNGG 6 cut(s) 755, 834, 887, 953, 1085, 1151
Mva1269I GAATGC 1 cut(s) 318
MvaI CCWGG 6 cut(s) 755, 834, 887, 953, 1085, 1151
MvnI CGCG 1 cut(s) 859
MwoI GCNNNNNNNGC 4 cut(s) 11, 27, 102, 264
NdeII GATC 3 cut(s) 280, 364, 601
NlaIII CATG 1 cut(s) 151
NmuCI GTSAC 1 cut(s) 213
NsiI ATGCAT 1 cut(s) 454
PctI GAATGC 1 cut(s) 318
PdmI GAANNNNTTC 2 cut(s) 141, 498
PfeI GAWTC 2 cut(s) 437, 476
PflFI GACNNNGTC 1 cut(s) 1151
PkrI GCNGC 1 cut(s) 7
PleI GAGTC 1 cut(s) 394
PpsI GAGTC 1 cut(s) 394
PshAI GACNNNNGTC 1 cut(s) 42
Psp124BI GAGCTC 1 cut(s) 69
Psp6I CCWGG 6 cut(s) 753, 832, 885, 951, 1083, 1149
PspFI CCCAGC 2 cut(s) 60, 87
PspGI CCWGG 6 cut(s) 753, 832, 885, 951, 1083, 1149
PspPI GGNCC 5 cut(s) 719, 851, 983, 1115, 1153
PsyI GACNNNGTC 1 cut(s) 1151
RsaI GTAC 2 cut(s) 338, 655
RsaNI GTAC 2 cut(s) 337, 654
RseI CAYNNNNRTG 1 cut(s) 225
SacI GAGCTC 1 cut(s) 69
SaqAI TTAA 2 cut(s) 54, 593
SatI GCNGC 1 cut(s) 6
Sau3AI GATC 3 cut(s) 280, 364, 601
Sau96I GGNCC 5 cut(s) 719, 851, 983, 1115, 1153
SchI GAGTC 1 cut(s) 395
ScrFI CCNGG 6 cut(s) 755, 834, 887, 953, 1085, 1151
SduI GDGCHC 1 cut(s) 69
SexAI ACCWGGT 1 cut(s) 1149
SfaNI GCATC 3 cut(s) 439, 461, 499
SfcI CTRYAG 5 cut(s) 729, 795, 927, 1059, 1125
SinI GGWCC 5 cut(s) 719, 851, 983, 1115, 1153
SmiMI CAYNNNNRTG 1 cut(s) 225
SmlI CTYRAG 1 cut(s) 68
SmoI CTYRAG 1 cut(s) 68
SsiI CCGC 2 cut(s) 105, 155
SspMI CTAG 1 cut(s) 272
SstI GAGCTC 1 cut(s) 69
StyD4I CCNGG 6 cut(s) 753, 832, 885, 951, 1083, 1149
StyI CCWWGG 1 cut(s) 701
TaaI ACNGT 5 cut(s) 41, 173, 355, 634, 653
TaqI TCGA 4 cut(s) 301, 384, 629, 766
TatI WGTACW 2 cut(s) 336, 653
TfiI GAWTC 2 cut(s) 437, 476
Tru1I TTAA 2 cut(s) 54, 593
Tru9I TTAA 2 cut(s) 54, 593
TscAI CASTG 3 cut(s) 46, 240, 496
TseFI GTSAC 1 cut(s) 213
TseI GCWGC 1 cut(s) 5
Tsp45I GTSAC 1 cut(s) 213
TspDTI ATGAA 1 cut(s) 348
TspGWI ACGGA 1 cut(s) 461
TspRI CASTG 3 cut(s) 46, 240, 496
Tth111I GACNNNGTC 1 cut(s) 1151
VpaK11BI GGWCC 5 cut(s) 719, 851, 983, 1115, 1153
XmnI GAANNNNTTC 2 cut(s) 141, 498
XspI CTAG 1 cut(s) 272
Zsp2I ATGCAT 1 cut(s) 454
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.