Rh5CG276900

B3 DNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0001196
Physical Location & Seq
Reverse (-)
30115320 .. 30125500
10181 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG276900.1

Sequence Viewer

Length: 1395 bp
ATGGCTGCCAAAGCTTCGTGCTTTTTCTGCAAGGCCGAGAGTGATCAGTTCGTTAATGGCTGGGAGCTCAAGGATGGGTCTTTCGCCCAGCTCTGCAAGAAATGCGGTTTTGCTTATGTGAATGGTATATTTTGTGAAACTTTTCACATGGAAGCAGAAGGCTGGAGAGACTGTGCTGTTTGTGGGAAGCCACTCCATTGTGGGTGCATTATGTCACGCCACAAGTATGCACCACTGGATTTCGGAGGTGTAGGTTGCACAAAATGCTTACTAGAAGTTGATCAATCTATTGCTTCAGGTCGAATGACCAGAATGCTGAAAGAAATAGTTGCCAAGTACAAGACGGACCCAACAGTTTCATCTGATCCTACTAAGAAAGTTGTCGAGTCCCTTTTTCCTGAATCTATACAAGCTCCAGCACCTCAGATTGGAGAAGAATCCAATACGGATGCATCTAAAGGAGATACAGCTTGTGAAGCCAAAGTAGGCACTGAACCACCTCAAGTGGATGCCTCCACAGGAAACCATTTACACCCTGACAATCTGCCGAAGATGACAAGGGCCGAGCAACTCCAAAAAGTCTCCAGAAACTTAAACTCTGATCTTATACCTCTGTTTGAGAAGTCATCAACTGTAAGTGATACTAATCACAGTACAGGATTTATTGTGATCCCGAAGAAGTATGCAGAGGAACAATCTCCGAGGGGCATTGAAACAGGTCCAACACCTGTACATACAAGTCTGAAAGCAGACCAGGATCAATCTCCATTGGATGTGGTAACAAATCCAATGCCTATAGAAACAAATCAGAAAACAAACCAGGAACAATCTCCAAAGGACAATGAAACAGGTCCAACGCGTGTAGATACAACTCTAAAACCAGACCAGGAACAATCTCCGATGGTTATGGAAACAAGTCCAATGCCTATAGAAACAAATCAGAAAACGAACCAGGAACAATCTCTAAGGGACTTTGAAACTGGTCCAACGCCTGTAGATACAAGTCTGAAAACAGACCAGGAACAGCCTCCAAAGGATATGGAGACAAGTCCAATGCCTGTAGAAACAAGTCATAAATCAGACCAGGGACAATCTCCAAAGGATATGGAAACAGGTCCAACGCCTGTAGAAACAAGTCTGAAAACAGACCAGGACGAATTGCTTGTATTTCGATATAAGCCGTACGATATTCCATCCCTAAGCTCCAAAGTCCCATTGGACAACGAAGCGTTCAGATTTCGGCTCAGCATGCTTGGCCTTGTAAGGCAATATATACGAATGCAAAGATCGATAGACCCACGGTACGTACCATCACTACCGGTACCATGGGAAGAACCACCTATGGCAAGAACCATGGTACGAGTCATGGGACGAAGTGTTCGAAGCGCTGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

464

Amino Acids

51.5

Weight (kDa)

5.23

Isoelectric Point (pI)

49.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf_VAL1_N PF25813 47 - 91 2.5e-21 VAL1-like, N-terminal zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1321
AccB1I GGYRCC 1 cut(s) 1321
AccII CGCG 1 cut(s) 859
AciI CCGC 1 cut(s) 105
AclWI GGATC 3 cut(s) 359, 664, 765
AcuI CTGAAG 1 cut(s) 279
AfaI GTAC 8 cut(s) 338, 655, 732, 1184, 1304, 1308, 1323, 1359
AfeI AGCGCT 1 cut(s) 1387
AfiI CCNNNNNNNGG 1 cut(s) 428
AflIII ACRYGT 1 cut(s) 857
AgeI ACCGGT 1 cut(s) 1318
AgsI TTSAA 2 cut(s) 713, 977
AjnI CCWGG 7 cut(s) 753, 819, 885, 951, 1017, 1083, 1149
AloI GAACNNNNNNTCC 2 cut(s) 1362, 1394
AluBI AGCT 6 cut(s) 14, 67, 91, 413, 470, 1203
AluI AGCT 6 cut(s) 14, 67, 91, 413, 470, 1203
Alw21I GWGCWC 1 cut(s) 69
Alw26I GTCTC 3 cut(s) 162, 586, 1037
AlwI GGATC 3 cut(s) 359, 664, 765
Aor51HI AGCGCT 1 cut(s) 1387
AoxI GGCC 3 cut(s) 33, 561, 1255
ApeKI GCWGC 1 cut(s) 5
ArsI GACNNNNNNTTYG 2 cut(s) 1362, 1394
AsiGI ACCGGT 1 cut(s) 1318
Asp700I GAANNNNTTC 1 cut(s) 141
Asp718I GGTACC 1 cut(s) 1321
AspLEI GCGC 1 cut(s) 1388
AspS9I GGNCC 6 cut(s) 346, 561, 719, 851, 983, 1115
AsuII TTCGAA 1 cut(s) 1381
AvaII GGWCC 5 cut(s) 346, 719, 851, 983, 1115
BanI GGYRCC 1 cut(s) 1321
BanII GRGCYC 1 cut(s) 69
Bbv12I GWGCWC 1 cut(s) 69
BccI CCATC 4 cut(s) 68, 895, 1201, 1318
BceAI ACGGC 1 cut(s) 1165
BciT130I CCWGG 7 cut(s) 755, 821, 887, 953, 1019, 1085, 1151
BclI TGATCA 2 cut(s) 43, 280
BcoDI GTCTC 3 cut(s) 162, 586, 1037
BfaI CTAG 1 cut(s) 272
BfmI CTRYAG 5 cut(s) 795, 927, 993, 1059, 1125
BfoI RGCGCY 1 cut(s) 1389
BisI GCNGC 1 cut(s) 6
BlpI GCTNAGC 1 cut(s) 1244
BlsI GCNGC 1 cut(s) 7
Bme1390I CCNGG 7 cut(s) 755, 821, 887, 953, 1019, 1085, 1151
Bme18I GGWCC 5 cut(s) 346, 719, 851, 983, 1115
BmgT120I GGNCC 6 cut(s) 346, 561, 719, 851, 983, 1115
BmiI GGNNCC 2 cut(s) 348, 1323
BmrFI CCNGG 7 cut(s) 755, 821, 887, 953, 1019, 1085, 1151
BmsI GCATC 3 cut(s) 439, 461, 499
BpmI CTGGAG 3 cut(s) 184, 399, 568
Bpu10I CCTNAGC 1 cut(s) 1199
Bpu1102I GCTNAGC 1 cut(s) 1244
Bpu14I TTCGAA 1 cut(s) 1381
BpuEI CTTGAG 2 cut(s) 53, 486
Bsa29I ATCGAT 1 cut(s) 1289
BsaAI YACGTR 1 cut(s) 1306
BsaBI GATNNNNATC 1 cut(s) 645
BsaJI CCNNGG 5 cut(s) 701, 1084, 1298, 1325, 1353
BsaWI WCCGGW 1 cut(s) 1318
BsaXI ACNNNNNCTCC 2 cut(s) 157, 187
Bsc4I CCNNNNNNNGG 1 cut(s) 428
Bse118I RCCGGY 1 cut(s) 1318
Bse1I ACTGG 2 cut(s) 240, 985
Bse8I GATNNNNATC 1 cut(s) 645
BseBI CCWGG 7 cut(s) 755, 821, 887, 953, 1019, 1085, 1151
BseCI ATCGAT 1 cut(s) 1289
BseDI CCNNGG 5 cut(s) 701, 1084, 1298, 1325, 1353
BseGI GGATG 5 cut(s) 79, 454, 514, 778, 1193
BseJI GATNNNNATC 1 cut(s) 645
BseLI CCNNNNNNNGG 1 cut(s) 428
BseMII CTCAG 2 cut(s) 437, 1258
BseNI ACTGG 2 cut(s) 240, 985
BseYI CCCAGC 2 cut(s) 60, 87
Bsh1236I CGCG 1 cut(s) 859
BshFI GGCC 3 cut(s) 35, 563, 1257
BshNI GGYRCC 1 cut(s) 1321
BshTI ACCGGT 1 cut(s) 1318
BshVI ATCGAT 1 cut(s) 1289
BsiHKAI GWGCWC 1 cut(s) 69
BsiSI CCGG 1 cut(s) 1319
BsiWI CGTACG 1 cut(s) 1182
BslFI GGGAC 5 cut(s) 373, 983, 1101, 1196, 1383
BslI CCNNNNNNNGG 1 cut(s) 428
BsmAI GTCTC 3 cut(s) 162, 586, 1037
BsmFI GGGAC 5 cut(s) 373, 983, 1101, 1196, 1383
BsmI GAATGC 2 cut(s) 318, 1284
BsnI GGCC 3 cut(s) 35, 563, 1257
Bsp119I TTCGAA 1 cut(s) 1381
Bsp1286I GDGCHC 1 cut(s) 69
Bsp1407I TGTACA 1 cut(s) 730
Bsp143I GATC 7 cut(s) 43, 280, 364, 601, 669, 757, 1286
Bsp1720I GCTNAGC 1 cut(s) 1244
Bsp19I CCATGG 2 cut(s) 1325, 1353
BspACI CCGC 1 cut(s) 105
BspANI GGCC 3 cut(s) 35, 563, 1257
BspCNI CTCAG 2 cut(s) 436, 1257
BspDI ATCGAT 1 cut(s) 1289
BspFNI CGCG 1 cut(s) 859
BspLI GGNNCC 2 cut(s) 348, 1323
BspPI GGATC 3 cut(s) 359, 664, 765
BspT104I TTCGAA 1 cut(s) 1381
BspT107I GGYRCC 1 cut(s) 1321
BsrFI RCCGGY 1 cut(s) 1318
BsrGI TGTACA 1 cut(s) 730
BsrI ACTGG 2 cut(s) 240, 985
BssAI RCCGGY 1 cut(s) 1318
BssECI CCNNGG 5 cut(s) 701, 1084, 1298, 1325, 1353
BssMI GATC 7 cut(s) 43, 280, 364, 601, 669, 757, 1286
BssT1I CCWWGG 2 cut(s) 1325, 1353
Bst2UI CCWGG 7 cut(s) 755, 821, 887, 953, 1019, 1085, 1151
Bst4CI ACNGT 5 cut(s) 173, 355, 634, 653, 1302
BstAPI GCANNNNNTGC 2 cut(s) 102, 264
BstAUI TGTACA 1 cut(s) 730
BstBAI YACGTR 1 cut(s) 1306
BstBI TTCGAA 1 cut(s) 1381
BstC8I GCNNGC 1 cut(s) 1250
BstDEI CTNAG 5 cut(s) 372, 423, 965, 1199, 1244
BstDSI CCRYGG 3 cut(s) 1298, 1325, 1353
BstF5I GGATG 5 cut(s) 79, 454, 514, 778, 1193
BstFNI CGCG 1 cut(s) 859
BstH2I RGCGCY 1 cut(s) 1389
BstHHI GCGC 1 cut(s) 1388
BstKTI GATC 7 cut(s) 46, 283, 367, 604, 672, 760, 1289
BstMAI GTCTC 3 cut(s) 162, 586, 1037
BstMBI GATC 7 cut(s) 43, 280, 364, 601, 669, 757, 1286
BstMWI GCNNNNNNNGC 7 cut(s) 11, 27, 102, 264, 476, 1249, 1254
BstNI CCWGG 7 cut(s) 755, 821, 887, 953, 1019, 1085, 1151
BstNSI RCATGY 1 cut(s) 1252
BstSCI CCNGG 7 cut(s) 753, 819, 885, 951, 1017, 1083, 1149
BstSFI CTRYAG 5 cut(s) 795, 927, 993, 1059, 1125
BstSNI TACGTA 1 cut(s) 1306
BstUI CGCG 1 cut(s) 859
Bsu15I ATCGAT 1 cut(s) 1289
BsuRI GGCC 3 cut(s) 35, 563, 1257
BsuTUI ATCGAT 1 cut(s) 1289
BtgI CCRYGG 3 cut(s) 1298, 1325, 1353
BtsCI GGATG 5 cut(s) 79, 454, 514, 778, 1193
BtsIMutI CAGTG 2 cut(s) 233, 489
Cac8I GCNNGC 1 cut(s) 1250
CfoI GCGC 1 cut(s) 1388
Cfr10I RCCGGY 1 cut(s) 1318
Cfr13I GGNCC 6 cut(s) 346, 561, 719, 851, 983, 1115
ClaI ATCGAT 1 cut(s) 1289
Csp6I GTAC 8 cut(s) 337, 654, 731, 1183, 1303, 1307, 1322, 1358
CspAI ACCGGT 1 cut(s) 1318
CviAII CATG 5 cut(s) 148, 1249, 1326, 1354, 1366
CviQI GTAC 8 cut(s) 337, 654, 731, 1183, 1303, 1307, 1322, 1358
DdeI CTNAG 5 cut(s) 372, 423, 965, 1199, 1244
DpnI GATC 7 cut(s) 45, 282, 366, 603, 671, 759, 1288
DpnII GATC 7 cut(s) 43, 280, 364, 601, 669, 757, 1286
Ecl136II GAGCTC 1 cut(s) 67
Eco105I TACGTA 1 cut(s) 1306
Eco130I CCWWGG 2 cut(s) 1325, 1353
Eco24I GRGCYC 1 cut(s) 69
Eco47I GGWCC 5 cut(s) 346, 719, 851, 983, 1115
Eco47III AGCGCT 1 cut(s) 1387
Eco53kI GAGCTC 1 cut(s) 67
Eco57I CTGAAG 1 cut(s) 279
EcoICRI GAGCTC 1 cut(s) 67
EcoRII CCWGG 7 cut(s) 753, 819, 885, 951, 1017, 1083, 1149
EcoT14I CCWWGG 2 cut(s) 1325, 1353
EcoT22I ATGCAT 1 cut(s) 454
EcoT38I GRGCYC 1 cut(s) 69
ErhI CCWWGG 2 cut(s) 1325, 1353
FaeI CATG 5 cut(s) 151, 1252, 1329, 1357, 1369
FaqI GGGAC 5 cut(s) 373, 983, 1101, 1196, 1383
FatI CATG 5 cut(s) 147, 1248, 1325, 1353, 1365
FbaI TGATCA 2 cut(s) 43, 280
Fnu4HI GCNGC 1 cut(s) 6
FokI GGATG 5 cut(s) 86, 461, 521, 785, 1180
FriOI GRGCYC 1 cut(s) 69
Fsp4HI GCNGC 1 cut(s) 6
FspBI CTAG 1 cut(s) 272
GlaI GCGC 1 cut(s) 1387
GluI GCNGC 1 cut(s) 6
GsaI CCCAGC 2 cut(s) 64, 91
GsuI CTGGAG 3 cut(s) 184, 399, 568
HaeII RGCGCY 1 cut(s) 1389
HaeIII GGCC 3 cut(s) 35, 563, 1257
HapII CCGG 1 cut(s) 1319
HhaI GCGC 1 cut(s) 1388
Hin1II CATG 5 cut(s) 151, 1252, 1329, 1357, 1369
Hin6I GCGC 1 cut(s) 1386
HinP1I GCGC 1 cut(s) 1386
HindIII AAGCTT 1 cut(s) 12
HinfI GANTC 4 cut(s) 386, 401, 437, 1362
HpaII CCGG 1 cut(s) 1319
Hpy188III TCNNGA 3 cut(s) 398, 585, 673
HpyAV CCTTC 1 cut(s) 152
HpyCH4III ACNGT 5 cut(s) 173, 355, 634, 653, 1302
HpyCH4IV ACGT 1 cut(s) 1305
HpyCH4V TGCA 8 cut(s) 30, 96, 207, 230, 258, 452, 686, 1282
HpyF10VI GCNNNNNNNGC 7 cut(s) 11, 27, 102, 264, 476, 1249, 1254
HpyF3I CTNAG 5 cut(s) 372, 423, 965, 1199, 1244
HpySE526I ACGT 1 cut(s) 1305
Hsp92II CATG 5 cut(s) 151, 1252, 1329, 1357, 1369
HspAI GCGC 1 cut(s) 1386
KpnI GGTACC 1 cut(s) 1325
Ksp22I TGATCA 2 cut(s) 43, 280
Kzo9I GATC 7 cut(s) 43, 280, 364, 601, 669, 757, 1286
LmnI GCTCC 3 cut(s) 64, 418, 1208
LweI GCATC 3 cut(s) 439, 461, 499
MaeI CTAG 1 cut(s) 272
MaeII ACGT 1 cut(s) 1305
MaeIII GTNAC 2 cut(s) 213, 778
MalI GATC 7 cut(s) 45, 282, 366, 603, 671, 759, 1288
MboI GATC 7 cut(s) 43, 280, 364, 601, 669, 757, 1286
MboII GAAGA 4 cut(s) 446, 562, 688, 1343
MhlI GDGCHC 1 cut(s) 69
MluCI AATT 1 cut(s) 1157
MluI ACGCGT 1 cut(s) 857
MlyI GAGTC 2 cut(s) 395, 1371
MmeI TCCRAC 4 cut(s) 746, 878, 1010, 1142
MnlI CCTC 8 cut(s) 239, 432, 510, 523, 621, 682, 696, 1038
Mph1103I ATGCAT 1 cut(s) 454
MroXI GAANNNNTTC 1 cut(s) 141
MseI TTAA 2 cut(s) 54, 593
MslI CAYNNNNRTG 1 cut(s) 225
MspI CCGG 1 cut(s) 1319
MspR9I CCNGG 7 cut(s) 755, 821, 887, 953, 1019, 1085, 1151
Mva1269I GAATGC 2 cut(s) 318, 1284
MvaI CCWGG 7 cut(s) 755, 821, 887, 953, 1019, 1085, 1151
MvnI CGCG 1 cut(s) 859
MwoI GCNNNNNNNGC 7 cut(s) 11, 27, 102, 264, 476, 1249, 1254
NcoI CCATGG 2 cut(s) 1325, 1353
NdeII GATC 7 cut(s) 43, 280, 364, 601, 669, 757, 1286
NlaIII CATG 5 cut(s) 151, 1252, 1329, 1357, 1369
NlaIV GGNNCC 2 cut(s) 348, 1323
NmeAIII GCCGAG 2 cut(s) 61, 589
NmuCI GTSAC 1 cut(s) 213
NsiI ATGCAT 1 cut(s) 454
NspI RCATGY 1 cut(s) 1252
NspV TTCGAA 1 cut(s) 1381
PaeI GCATGC 1 cut(s) 1252
PcsI WCGNNNNNNNCGW 1 cut(s) 1378
PctI GAATGC 2 cut(s) 318, 1284
PdmI GAANNNNTTC 1 cut(s) 141
PfeI GAWTC 2 cut(s) 401, 437
Pfl23II CGTACG 1 cut(s) 1182
PinAI ACCGGT 1 cut(s) 1318
PkrI GCNGC 1 cut(s) 7
PleI GAGTC 2 cut(s) 394, 1370
PpsI GAGTC 2 cut(s) 394, 1370
Ppu21I YACGTR 1 cut(s) 1306
Psp124BI GAGCTC 1 cut(s) 69
Psp6I CCWGG 7 cut(s) 753, 819, 885, 951, 1017, 1083, 1149
PspFI CCCAGC 2 cut(s) 60, 87
PspGI CCWGG 7 cut(s) 753, 819, 885, 951, 1017, 1083, 1149
PspLI CGTACG 1 cut(s) 1182
PspN4I GGNNCC 2 cut(s) 348, 1323
PspPI GGNCC 6 cut(s) 346, 561, 719, 851, 983, 1115
RsaI GTAC 8 cut(s) 338, 655, 732, 1184, 1304, 1308, 1323, 1359
RsaNI GTAC 8 cut(s) 337, 654, 731, 1183, 1303, 1307, 1322, 1358
RseI CAYNNNNRTG 1 cut(s) 225
SacI GAGCTC 1 cut(s) 69
SaqAI TTAA 2 cut(s) 54, 593
SatI GCNGC 1 cut(s) 6
Sau3AI GATC 7 cut(s) 43, 280, 364, 601, 669, 757, 1286
Sau96I GGNCC 6 cut(s) 346, 561, 719, 851, 983, 1115
SchI GAGTC 2 cut(s) 395, 1371
ScrFI CCNGG 7 cut(s) 755, 821, 887, 953, 1019, 1085, 1151
SduI GDGCHC 1 cut(s) 69
SfaNI GCATC 3 cut(s) 439, 461, 499
SfcI CTRYAG 5 cut(s) 795, 927, 993, 1059, 1125
SfuI TTCGAA 1 cut(s) 1381
SinI GGWCC 5 cut(s) 346, 719, 851, 983, 1115
SmiMI CAYNNNNRTG 1 cut(s) 225
SmlI CTYRAG 2 cut(s) 68, 501
SmoI CTYRAG 2 cut(s) 68, 501
SnaBI TACGTA 1 cut(s) 1306
SphI GCATGC 1 cut(s) 1252
Sse9I AATT 1 cut(s) 1157
SsiI CCGC 1 cut(s) 105
SspMI CTAG 1 cut(s) 272
SstI GAGCTC 1 cut(s) 69
StyD4I CCNGG 7 cut(s) 753, 819, 885, 951, 1017, 1083, 1149
StyI CCWWGG 2 cut(s) 1325, 1353
TaaI ACNGT 5 cut(s) 173, 355, 634, 653, 1302
TaiI ACGT 1 cut(s) 1308
TaqI TCGA 5 cut(s) 301, 384, 1171, 1289, 1381
TasI AATT 1 cut(s) 1157
TatI WGTACW 3 cut(s) 336, 653, 730
TfiI GAWTC 2 cut(s) 401, 437
Tru1I TTAA 2 cut(s) 54, 593
Tru9I TTAA 2 cut(s) 54, 593
TscAI CASTG 2 cut(s) 240, 496
TseFI GTSAC 1 cut(s) 213
TseI GCWGC 1 cut(s) 5
Tsp45I GTSAC 1 cut(s) 213
TspDTI ATGAA 2 cut(s) 348, 858
TspGWI ACGGA 2 cut(s) 359, 461
TspRI CASTG 2 cut(s) 240, 496
VpaK11BI GGWCC 5 cut(s) 346, 719, 851, 983, 1115
XceI RCATGY 1 cut(s) 1252
XcmI CCANNNNNNNNNTGG 1 cut(s) 1213
XmnI GAANNNNTTC 1 cut(s) 141
XspI CTAG 1 cut(s) 272
Zsp2I ATGCAT 1 cut(s) 454
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.