Rmu_sc0009046.1_g000009

B3 DNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0009046.1
OG0001196
Physical Location & Seq
Reverse (-)
39274 .. 45565
6292 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0009046.1_g000009.1.cds

Sequence Viewer

Length: 1044 bp
atggctgccaaagcttcgtgcttttcctgcaaggtcgagagtgatcagcttgttaatggctgggagctcaaggatgggtctttcgcccagctttgcaagaaatgcggttttgcttatgtgaatggtatattttgtgaaacttttcacatggaagcagaaagctggagagactgtgctgtttgtgggaagccactccattgtgggtgcattatgtcacgccacaagtatgcaccactggatttcggaggtgtaggttgcacaaaatgcttactagaagttgatcaatctattgcttcaggtcgaatgaccagaatggtgaaagacatagttgccaagtacaagacggacccaacagtttcatctgatcctactaagaaagttgttgagtccctttttcctgaatctttacaagctccagcacctcagattggagaagaatccaatacggatgtatctaaaggagatacagcttgtgaattcaaagtagacagtgaaaaacctcaagtggatgcctccacaggaaaccattcacaccctgacaatctgctgaagatgacaagggctgagcaactccaaaaagtctccggaaatacaggatttcttgtgataccgaagaaatatgcagaggaacaatctccaagggacattgaaacaggtccaacacctgtatatacaagtctgagagcagaccaggaacaatctccaatggatgtggtaacaaatccaatgcctatagaaacaaatcagaaaacaaaccaggaacaatctccaagggacattgaaacaggtccaacgcgtgtagatacaaccctgaaaccagaccaggaacaatctctaagggactttgaaacaggtccaacgtctgtagatacaagtctgaaaacagaccaggaacaaccttcaaaggatatggagacaagtccaatgcctgtagaaacaagtcttaaatcagaccagggacaatctccaaaggatatggaaacaggtccaacgcctgtagaatcaagtctgaaaacagaccaggttcaactcctgaagaaataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

347

Amino Acids

38.12

Weight (kDa)

4.95

Isoelectric Point (pI)

47.22

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 486
AccII CGCG 1 cut(s) 796
AccIII TCCGGA 1 cut(s) 584
AciI CCGC 1 cut(s) 105
AclWI GGATC 1 cut(s) 359
AcsI RAATTY 1 cut(s) 476
AcuI CTGAAG 2 cut(s) 279, 569
AfaI GTAC 1 cut(s) 338
AfiI CCNNNNNNNGG 1 cut(s) 428
AflIII ACRYGT 1 cut(s) 794
AgsI TTSAA 6 cut(s) 481, 650, 782, 848, 903, 1028
AjnI CCWGG 6 cut(s) 690, 756, 822, 888, 954, 1020
AluBI AGCT 7 cut(s) 14, 49, 67, 91, 162, 413, 470
AluI AGCT 7 cut(s) 14, 49, 67, 91, 162, 413, 470
Alw21I GWGCWC 1 cut(s) 69
Alw26I GTCTC 3 cut(s) 162, 586, 908
AlwI GGATC 1 cut(s) 359
Aor13HI TCCGGA 1 cut(s) 584
ApeKI GCWGC 1 cut(s) 5
ApoI RAATTY 1 cut(s) 476
Asp700I GAANNNNTTC 2 cut(s) 141, 526
AspS9I GGNCC 5 cut(s) 346, 656, 788, 854, 986
AsuHPI GGTGA 1 cut(s) 328
AvaII GGWCC 5 cut(s) 346, 656, 788, 854, 986
BaeI ACNNNNGTAYC 2 cut(s) 435, 468
BanII GRGCYC 1 cut(s) 69
Bbv12I GWGCWC 1 cut(s) 69
BccI CCATC 1 cut(s) 68
BciT130I CCWGG 6 cut(s) 692, 758, 824, 890, 956, 1022
BclI TGATCA 2 cut(s) 43, 280
BcoDI GTCTC 3 cut(s) 162, 586, 908
BfaI CTAG 1 cut(s) 272
BfmI CTRYAG 4 cut(s) 732, 864, 930, 996
BisI GCNGC 1 cut(s) 6
BlpI GCTNAGC 1 cut(s) 564
BlsI GCNGC 1 cut(s) 7
Bme1390I CCNGG 6 cut(s) 692, 758, 824, 890, 956, 1022
Bme18I GGWCC 5 cut(s) 346, 656, 788, 854, 986
BmgT120I GGNCC 5 cut(s) 346, 656, 788, 854, 986
BmiI GGNNCC 1 cut(s) 348
BmrFI CCNGG 6 cut(s) 692, 758, 824, 890, 956, 1022
BmsI GCATC 1 cut(s) 499
BpmI CTGGAG 2 cut(s) 184, 399
Bpu1102I GCTNAGC 1 cut(s) 564
BpuEI CTTGAG 2 cut(s) 53, 486
BsaJI CCNNGG 3 cut(s) 638, 770, 955
BsaWI WCCGGW 1 cut(s) 584
BsaXI ACNNNNNCTCC 2 cut(s) 157, 187
Bsc4I CCNNNNNNNGG 1 cut(s) 428
Bse1I ACTGG 1 cut(s) 240
BseAI TCCGGA 1 cut(s) 584
BseBI CCWGG 6 cut(s) 692, 758, 824, 890, 956, 1022
BseDI CCNNGG 3 cut(s) 638, 770, 955
BseGI GGATG 4 cut(s) 79, 454, 514, 715
BseLI CCNNNNNNNGG 1 cut(s) 428
BseMII CTCAG 3 cut(s) 437, 555, 671
BseNI ACTGG 1 cut(s) 240
BseYI CCCAGC 2 cut(s) 60, 87
Bsh1236I CGCG 1 cut(s) 796
BsiHKAI GWGCWC 1 cut(s) 69
BsiSI CCGG 1 cut(s) 585
BslFI GGGAC 5 cut(s) 373, 656, 788, 854, 972
BslI CCNNNNNNNGG 1 cut(s) 428
BsmAI GTCTC 3 cut(s) 162, 586, 908
BsmFI GGGAC 5 cut(s) 373, 656, 788, 854, 972
Bsp1286I GDGCHC 1 cut(s) 69
Bsp13I TCCGGA 1 cut(s) 584
Bsp143I GATC 3 cut(s) 43, 280, 364
Bsp1720I GCTNAGC 1 cut(s) 564
BspACI CCGC 1 cut(s) 105
BspCNI CTCAG 3 cut(s) 436, 556, 672
BspEI TCCGGA 1 cut(s) 584
BspFNI CGCG 1 cut(s) 796
BspLI GGNNCC 1 cut(s) 348
BspPI GGATC 1 cut(s) 359
BsrI ACTGG 1 cut(s) 240
BssECI CCNNGG 3 cut(s) 638, 770, 955
BssMI GATC 3 cut(s) 43, 280, 364
BssT1I CCWWGG 2 cut(s) 638, 770
Bst2UI CCWGG 6 cut(s) 692, 758, 824, 890, 956, 1022
Bst4CI ACNGT 3 cut(s) 173, 355, 491
BstAPI GCANNNNNTGC 2 cut(s) 102, 264
BstDEI CTNAG 5 cut(s) 372, 423, 564, 680, 836
BstF5I GGATG 4 cut(s) 79, 454, 514, 715
BstFNI CGCG 1 cut(s) 796
BstKTI GATC 3 cut(s) 46, 283, 367
BstMAI GTCTC 3 cut(s) 162, 586, 908
BstMBI GATC 3 cut(s) 43, 280, 364
BstMWI GCNNNNNNNGC 4 cut(s) 11, 27, 102, 264
BstNI CCWGG 6 cut(s) 692, 758, 824, 890, 956, 1022
BstSCI CCNGG 6 cut(s) 690, 756, 822, 888, 954, 1020
BstSFI CTRYAG 4 cut(s) 732, 864, 930, 996
BstUI CGCG 1 cut(s) 796
BtsCI GGATG 4 cut(s) 79, 454, 514, 715
BtsIMutI CAGTG 2 cut(s) 233, 496
Cfr13I GGNCC 5 cut(s) 346, 656, 788, 854, 986
CsiI ACCWGGT 1 cut(s) 1020
Csp6I GTAC 1 cut(s) 337
CspCI CAANNNNNGTGG 2 cut(s) 693, 728
CviAII CATG 1 cut(s) 148
CviQI GTAC 1 cut(s) 337
DdeI CTNAG 5 cut(s) 372, 423, 564, 680, 836
DpnI GATC 3 cut(s) 45, 282, 366
DpnII GATC 3 cut(s) 43, 280, 364
Ecl136II GAGCTC 1 cut(s) 67
Eco130I CCWWGG 2 cut(s) 638, 770
Eco24I GRGCYC 1 cut(s) 69
Eco47I GGWCC 5 cut(s) 346, 656, 788, 854, 986
Eco53kI GAGCTC 1 cut(s) 67
Eco57I CTGAAG 2 cut(s) 279, 569
EcoICRI GAGCTC 1 cut(s) 67
EcoRI GAATTC 1 cut(s) 476
EcoRII CCWGG 6 cut(s) 690, 756, 822, 888, 954, 1020
EcoT14I CCWWGG 2 cut(s) 638, 770
EcoT38I GRGCYC 1 cut(s) 69
ErhI CCWWGG 2 cut(s) 638, 770
FaeI CATG 1 cut(s) 151
FaqI GGGAC 5 cut(s) 373, 656, 788, 854, 972
FatI CATG 1 cut(s) 147
FbaI TGATCA 2 cut(s) 43, 280
FblI GTMKAC 1 cut(s) 486
Fnu4HI GCNGC 1 cut(s) 6
FokI GGATG 4 cut(s) 86, 461, 521, 722
FriOI GRGCYC 1 cut(s) 69
Fsp4HI GCNGC 1 cut(s) 6
FspBI CTAG 1 cut(s) 272
GluI GCNGC 1 cut(s) 6
GsaI CCCAGC 2 cut(s) 64, 91
GsuI CTGGAG 2 cut(s) 184, 399
HapII CCGG 1 cut(s) 585
Hin1II CATG 1 cut(s) 151
HindIII AAGCTT 1 cut(s) 12
HinfI GANTC 4 cut(s) 386, 401, 437, 1001
HpaII CCGG 1 cut(s) 585
HphI GGTGA 1 cut(s) 328
Hpy166II GTNNAC 1 cut(s) 487
Hpy188I TCNGA 8 cut(s) 245, 364, 426, 681, 747, 879, 952, 1011
Hpy188III TCNNGA 4 cut(s) 37, 398, 585, 1033
Hpy8I GTNNAC 1 cut(s) 487
HpyAV CCTTC 1 cut(s) 909
HpyCH4III ACNGT 3 cut(s) 173, 355, 491
HpyCH4IV ACGT 1 cut(s) 860
HpyCH4V TGCA 6 cut(s) 30, 96, 207, 230, 258, 623
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 27, 102, 264
HpyF3I CTNAG 5 cut(s) 372, 423, 564, 680, 836
HpySE526I ACGT 1 cut(s) 860
Hsp92II CATG 1 cut(s) 151
Kpn2I TCCGGA 1 cut(s) 584
Ksp22I TGATCA 2 cut(s) 43, 280
Kzo9I GATC 3 cut(s) 43, 280, 364
LmnI GCTCC 2 cut(s) 64, 418
LweI GCATC 1 cut(s) 499
MabI ACCWGGT 1 cut(s) 1020
MaeI CTAG 1 cut(s) 272
MaeII ACGT 1 cut(s) 860
MaeIII GTNAC 2 cut(s) 213, 715
MalI GATC 3 cut(s) 45, 282, 366
MboI GATC 3 cut(s) 43, 280, 364
MboII GAAGA 3 cut(s) 446, 562, 625
MhlI GDGCHC 1 cut(s) 69
MluCI AATT 1 cut(s) 476
MluI ACGCGT 1 cut(s) 794
MlyI GAGTC 1 cut(s) 395
MmeI TCCRAC 4 cut(s) 683, 815, 881, 1013
MnlI CCTC 5 cut(s) 239, 432, 510, 523, 619
MroI TCCGGA 1 cut(s) 584
MroXI GAANNNNTTC 2 cut(s) 141, 526
MseI TTAA 2 cut(s) 54, 945
MslI CAYNNNNRTG 1 cut(s) 225
MspI CCGG 1 cut(s) 585
MspR9I CCNGG 6 cut(s) 692, 758, 824, 890, 956, 1022
MvaI CCWGG 6 cut(s) 692, 758, 824, 890, 956, 1022
MvnI CGCG 1 cut(s) 796
MwoI GCNNNNNNNGC 4 cut(s) 11, 27, 102, 264
NdeII GATC 3 cut(s) 43, 280, 364
NlaIII CATG 1 cut(s) 151
NlaIV GGNNCC 1 cut(s) 348
NmuCI GTSAC 1 cut(s) 213
PdmI GAANNNNTTC 2 cut(s) 141, 526
PfeI GAWTC 3 cut(s) 401, 437, 1001
PkrI GCNGC 1 cut(s) 7
PleI GAGTC 1 cut(s) 394
PpsI GAGTC 1 cut(s) 394
Psp124BI GAGCTC 1 cut(s) 69
Psp6I CCWGG 6 cut(s) 690, 756, 822, 888, 954, 1020
PspFI CCCAGC 2 cut(s) 60, 87
PspGI CCWGG 6 cut(s) 690, 756, 822, 888, 954, 1020
PspN4I GGNNCC 1 cut(s) 348
PspPI GGNCC 5 cut(s) 346, 656, 788, 854, 986
RsaI GTAC 1 cut(s) 338
RsaNI GTAC 1 cut(s) 337
RseI CAYNNNNRTG 1 cut(s) 225
SacI GAGCTC 1 cut(s) 69
SaqAI TTAA 2 cut(s) 54, 945
SatI GCNGC 1 cut(s) 6
Sau3AI GATC 3 cut(s) 43, 280, 364
Sau96I GGNCC 5 cut(s) 346, 656, 788, 854, 986
SchI GAGTC 1 cut(s) 395
ScrFI CCNGG 6 cut(s) 692, 758, 824, 890, 956, 1022
SduI GDGCHC 1 cut(s) 69
SexAI ACCWGGT 1 cut(s) 1020
SfaNI GCATC 1 cut(s) 499
SfcI CTRYAG 4 cut(s) 732, 864, 930, 996
SinI GGWCC 5 cut(s) 346, 656, 788, 854, 986
SmiMI CAYNNNNRTG 1 cut(s) 225
SmlI CTYRAG 2 cut(s) 68, 501
SmoI CTYRAG 2 cut(s) 68, 501
Sse9I AATT 1 cut(s) 476
SsiI CCGC 1 cut(s) 105
SspMI CTAG 1 cut(s) 272
SstI GAGCTC 1 cut(s) 69
StyD4I CCNGG 6 cut(s) 690, 756, 822, 888, 954, 1020
StyI CCWWGG 2 cut(s) 638, 770
TaaI ACNGT 3 cut(s) 173, 355, 491
TaiI ACGT 1 cut(s) 863
TaqI TCGA 2 cut(s) 36, 301
TasI AATT 1 cut(s) 476
TatI WGTACW 1 cut(s) 336
TfiI GAWTC 3 cut(s) 401, 437, 1001
Tru1I TTAA 2 cut(s) 54, 945
Tru9I TTAA 2 cut(s) 54, 945
TscAI CASTG 2 cut(s) 240, 496
TseFI GTSAC 1 cut(s) 213
TseI GCWGC 1 cut(s) 5
Tsp45I GTSAC 1 cut(s) 213
TspDTI ATGAA 1 cut(s) 348
TspGWI ACGGA 2 cut(s) 359, 461
TspRI CASTG 2 cut(s) 240, 496
VpaK11BI GGWCC 5 cut(s) 346, 656, 788, 854, 986
XapI RAATTY 1 cut(s) 476
XmiI GTMKAC 1 cut(s) 486
XmnI GAANNNNTTC 2 cut(s) 141, 526
XspI CTAG 1 cut(s) 272
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.