RLG00000033655

B3 DNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0001196
Physical Location & Seq
Reverse (-)
30258425 .. 30271200
12776 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000033655

Sequence Viewer

Length: 1800 bp
ATGGCTGCCAAAGCTTCGTGCTTTTCGTGCAAGGCCGAGATTGATCAGTTCGTTAATGGCTGGGAGCTCAAGGATGGGTCTTTGGCCCAGCTCTGCAAGAAATGCGGTTTTGCTTATGTGAATGGCATATTTTGTGAAACTTTTCACATGGAAGCGGAAGGCTGGAGAGACTGTGCTGTTTGTGGGAAGCCACTCCATTGTGGGTGCATTATGTCACTCCACAAGTATGCACCACTGGATTTCGGAGGTGTAGGTTGCACAAAATGCTTACTAGAAGTTGATCAATCTATTGCTTCAGGTCAAATGACCAGAATGCTGAAAGAAATAGTTGCGAAGTACAAGACTGACCCAACAGTTTCATCTGATCCTACTAAGAAAGTTGTCGAGTCCCTTTTTCCTGAATCTTTACAAGCTCCAGCACCTCAGATTGGAGAAGAATCCAATACGGATGCATCTAAAGGAGATACAGCTTGTGAAGCCAAAGTAGGCACTGAAACACCTCAAGTGGATGCCTCCACAGGAAACCATTTACACCCTGACAATCTGCTGAAGATGACAAGGGCTGAGCAACTCCAGAAAGTCTCCAGAAACTTAAACTCTGATCTTATACCTCTGTTTGAGAAGTCATCAACCGTAAGTGATACTAATCATAGTACAGGATTTCTTGTGATACCGAAGAAATATGCAGAGGAACAATCTCCAAGGGACAGAGAAACAGGTCCAACACCTGTAGATACAAGTCTGAAAGCAGACCAGGAACAACCTCCAATGGATGTGGCAACAAATCCAATGCCTATAGAAACAAATCAGAAAACAAACCAGGAACAATCTCCAAGGGACAATGAAACAAGTCCAACGCGTGTAGATACAACTCTGAAACCAGACCAGGAACAATCTCCGATGGTTATGGAAACAAGTCCAATGCCTATAGAAACAAATCAGGAAACGAACCAGGAACAATCTCTAAGGGACTTTGAAACAGGTCCAACGCCTGTAGATACAAGTCTGAAAACAGACCAGGAACAACCTCCAAAGGATATGGAGACAAGTCCAATGCCTGTAGAAACACGTCATATATCAGACCAGGGACAATCTCTGAAGGATATGGAAACAGGTCCAACGCCAGTAGAAACAAGTCTGAAAACAGACCAGGGTACAGATGCGGATCGACCTGATCTTAGGGTTATACCAGTTCAGCGACCCCGAAACCACATGCGACTCCAAAGTCAAACCCGAACGCCTGAAGCAAAACTCATTCGAGAAGTTGTTGATGAGATATGGAGGAAATTGCATTCCTCTTTTTCAAAACCTGCCATTAAGAATCTGGCATCAACGCCTTCTTGGTCTTCTTCTTGTCGACGGAAGCATGATGTATTTCTAAGCTTTAGAGGGAAGGATACCCGCTTGAATTTTTTGGACCATCTATATGATGCATTAATTCAGTGCAGAATTTCCACTTTTAGAGATGACAAAGATCTTGTTAAAGGAATGTCCATTCTCAAACAACTCGAAAAAGCAATTGAGGGATCAAAGGTTTGGGTTGTCATTGTTTCACCAAACTATGCTTCTTCAAAATGGTGCTTGGATGAGCTTTTGAAGATTCTTGAATGCAAAAAAGCATATAAAGTGGGGAAGAATAGAAGTTTGATGAAGGCATTTGCAGAATATGAAGAACAAGCTCATACTTTGGTGAATGGAGACAGAACACATAAGGGGAAGGCTAGTTTGCGGAACAAGAACAACAGTACTAATCATAATAAAAGGTTATTCATCATTTTCAGTTTCACGCAAGTTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

600

Amino Acids

67.06

Weight (kDa)

6.21

Isoelectric Point (pI)

44.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf_VAL1_N PF25813 47 - 91 5.3e-21 VAL1-like, N-terminal zinc finger domain
TIR PF01582 455 - 541 1.6e-30 TIR domain
TIR_2 PF13676 458 - 538 3.5e-14 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1224
Acc36I ACCTGC 1 cut(s) 1318
AccI GTMKAC 1 cut(s) 1357
AccII CGCG 1 cut(s) 859
AciI CCGC 5 cut(s) 105, 155, 1163, 1402, 1729
AclWI GGATC 3 cut(s) 359, 1173, 1534
AcsI RAATTY 2 cut(s) 1408, 1449
AcuI CTGAAG 4 cut(s) 279, 569, 1118, 1263
AfaI GTAC 4 cut(s) 338, 655, 1156, 1747
AfiI CCNNNNNNNGG 1 cut(s) 428
AflIII ACRYGT 2 cut(s) 857, 1067
AgsI TTSAA 6 cut(s) 977, 1305, 1408, 1572, 1597, 1607
AjiI CACGTC 1 cut(s) 1070
AjnI CCWGG 7 cut(s) 753, 819, 885, 951, 1017, 1083, 1149
AjuI GAANNNNNNNTTGG 2 cut(s) 1549, 1581
AluBI AGCT 8 cut(s) 14, 67, 91, 413, 470, 1383, 1591, 1679
AluI AGCT 8 cut(s) 14, 67, 91, 413, 470, 1383, 1591, 1679
Alw21I GWGCWC 1 cut(s) 69
Alw26I GTCTC 4 cut(s) 162, 586, 1037, 1692
AlwI GGATC 3 cut(s) 359, 1173, 1534
AoxI GGCC 2 cut(s) 33, 84
ApeKI GCWGC 1 cut(s) 5
ApoI RAATTY 2 cut(s) 1408, 1449
AseI ATTAAT 1 cut(s) 1436
Asp700I GAANNNNTTC 1 cut(s) 141
AspS9I GGNCC 5 cut(s) 85, 719, 983, 1115, 1417
AsuHPI GGTGA 2 cut(s) 1545, 1702
AvaII GGWCC 4 cut(s) 719, 983, 1115, 1417
BanII GRGCYC 1 cut(s) 69
BbsI GAAGAC 1 cut(s) 1338
Bbv12I GWGCWC 1 cut(s) 69
BccI CCATC 3 cut(s) 68, 895, 1428
BciT130I CCWGG 7 cut(s) 755, 821, 887, 953, 1019, 1085, 1151
BciVI GTATCC 1 cut(s) 1390
BclI TGATCA 2 cut(s) 43, 280
BcoDI GTCTC 4 cut(s) 162, 586, 1037, 1692
BfaI CTAG 2 cut(s) 272, 1722
BfmI CTRYAG 5 cut(s) 729, 795, 927, 993, 1059
BfuAI ACCTGC 1 cut(s) 1318
BfuI GTATCC 1 cut(s) 1390
BglII AGATCT 1 cut(s) 1474
BisI GCNGC 1 cut(s) 6
BlpI GCTNAGC 1 cut(s) 564
BlsI GCNGC 1 cut(s) 7
BmcAI AGTACT 1 cut(s) 1747
Bme1390I CCNGG 7 cut(s) 755, 821, 887, 953, 1019, 1085, 1151
Bme18I GGWCC 4 cut(s) 719, 983, 1115, 1417
BmgBI CACGTC 1 cut(s) 1070
BmgT120I GGNCC 5 cut(s) 85, 719, 983, 1115, 1417
BmrFI CCNGG 7 cut(s) 755, 821, 887, 953, 1019, 1085, 1151
BmsI GCATC 6 cut(s) 439, 461, 499, 1150, 1337, 1420
BpiI GAAGAC 1 cut(s) 1338
BpmI CTGGAG 4 cut(s) 184, 399, 557, 568
Bpu1102I GCTNAGC 1 cut(s) 564
BpuEI CTTGAG 2 cut(s) 53, 486
BsaBI GATNNNNATC 2 cut(s) 645, 1164
BsaJI CCNNGG 4 cut(s) 701, 833, 1084, 1150
BsaXI ACNNNNNCTCC 2 cut(s) 157, 187
Bsc4I CCNNNNNNNGG 1 cut(s) 428
Bse1I ACTGG 3 cut(s) 240, 1124, 1190
Bse8I GATNNNNATC 2 cut(s) 645, 1164
BseBI CCWGG 7 cut(s) 755, 821, 887, 953, 1019, 1085, 1151
BseDI CCNNGG 4 cut(s) 701, 833, 1084, 1150
BseGI GGATG 5 cut(s) 79, 454, 514, 778, 1591
BseJI GATNNNNATC 2 cut(s) 645, 1164
BseLI CCNNNNNNNGG 1 cut(s) 428
BseMII CTCAG 2 cut(s) 437, 555
BseNI ACTGG 3 cut(s) 240, 1124, 1190
BseYI CCCAGC 2 cut(s) 60, 87
BsgI GTGCAG 1 cut(s) 1465
Bsh1236I CGCG 1 cut(s) 859
BshFI GGCC 2 cut(s) 35, 86
BsiHKAI GWGCWC 1 cut(s) 69
BslFI GGGAC 5 cut(s) 373, 719, 851, 983, 1101
BslI CCNNNNNNNGG 1 cut(s) 428
BsmAI GTCTC 4 cut(s) 162, 586, 1037, 1692
BsmFI GGGAC 5 cut(s) 373, 719, 851, 983, 1101
BsmI GAATGC 3 cut(s) 318, 1291, 1613
BsnI GGCC 2 cut(s) 35, 86
Bsp1286I GDGCHC 1 cut(s) 69
Bsp143I GATC 8 cut(s) 43, 280, 364, 601, 1165, 1174, 1474, 1526
Bsp1720I GCTNAGC 1 cut(s) 564
BspACI CCGC 5 cut(s) 105, 155, 1163, 1402, 1729
BspANI GGCC 2 cut(s) 35, 86
BspCNI CTCAG 2 cut(s) 436, 556
BspFNI CGCG 1 cut(s) 859
BspMI ACCTGC 1 cut(s) 1318
BspPI GGATC 3 cut(s) 359, 1173, 1534
BsrI ACTGG 3 cut(s) 240, 1124, 1190
BssECI CCNNGG 4 cut(s) 701, 833, 1084, 1150
BssMI GATC 8 cut(s) 43, 280, 364, 601, 1165, 1174, 1474, 1526
BssT1I CCWWGG 2 cut(s) 701, 833
Bst2UI CCWGG 7 cut(s) 755, 821, 887, 953, 1019, 1085, 1151
Bst4CI ACNGT 4 cut(s) 173, 355, 634, 1745
BstAPI GCANNNNNTGC 2 cut(s) 102, 264
BstDEI CTNAG 6 cut(s) 372, 423, 564, 965, 1178, 1379
BstF5I GGATG 5 cut(s) 79, 454, 514, 778, 1591
BstFNI CGCG 1 cut(s) 859
BstKTI GATC 8 cut(s) 46, 283, 367, 604, 1168, 1177, 1477, 1529
BstMAI GTCTC 4 cut(s) 162, 586, 1037, 1692
BstMBI GATC 8 cut(s) 43, 280, 364, 601, 1165, 1174, 1474, 1526
BstMWI GCNNNNNNNGC 5 cut(s) 11, 27, 102, 264, 476
BstNI CCWGG 7 cut(s) 755, 821, 887, 953, 1019, 1085, 1151
BstNSI RCATGY 1 cut(s) 1216
BstSCI CCNGG 7 cut(s) 753, 819, 885, 951, 1017, 1083, 1149
BstSFI CTRYAG 5 cut(s) 729, 795, 927, 993, 1059
BstUI CGCG 1 cut(s) 859
BstV2I GAAGAC 1 cut(s) 1338
BstX2I RGATCY 1 cut(s) 1474
BstYI RGATCY 1 cut(s) 1474
BsuI GTATCC 1 cut(s) 1390
BsuRI GGCC 2 cut(s) 35, 86
BtrI CACGTC 1 cut(s) 1070
BtsCI GGATG 5 cut(s) 79, 454, 514, 778, 1591
BtsIMutI CAGTG 3 cut(s) 233, 489, 1448
BveI ACCTGC 1 cut(s) 1318
Cfr13I GGNCC 5 cut(s) 85, 719, 983, 1115, 1417
Csp6I GTAC 4 cut(s) 337, 654, 1155, 1746
CspCI CAANNNNNGTGG 2 cut(s) 756, 791
CviAII CATG 3 cut(s) 148, 1213, 1367
CviQI GTAC 4 cut(s) 337, 654, 1155, 1746
DdeI CTNAG 6 cut(s) 372, 423, 564, 965, 1178, 1379
DpnI GATC 8 cut(s) 45, 282, 366, 603, 1167, 1176, 1476, 1528
DpnII GATC 8 cut(s) 43, 280, 364, 601, 1165, 1174, 1474, 1526
DrdI GACNNNNNNGTC 1 cut(s) 1224
DseDI GACNNNNNNGTC 1 cut(s) 1224
Ecl136II GAGCTC 1 cut(s) 67
Eco130I CCWWGG 2 cut(s) 701, 833
Eco24I GRGCYC 1 cut(s) 69
Eco47I GGWCC 4 cut(s) 719, 983, 1115, 1417
Eco53kI GAGCTC 1 cut(s) 67
Eco57I CTGAAG 4 cut(s) 279, 569, 1118, 1263
EcoICRI GAGCTC 1 cut(s) 67
EcoRII CCWGG 7 cut(s) 753, 819, 885, 951, 1017, 1083, 1149
EcoT14I CCWWGG 2 cut(s) 701, 833
EcoT22I ATGCAT 2 cut(s) 454, 1435
EcoT38I GRGCYC 1 cut(s) 69
ErhI CCWWGG 2 cut(s) 701, 833
FaeI CATG 3 cut(s) 151, 1216, 1370
FaqI GGGAC 5 cut(s) 373, 719, 851, 983, 1101
FatI CATG 3 cut(s) 147, 1212, 1366
FauI CCCGC 1 cut(s) 1409
FbaI TGATCA 2 cut(s) 43, 280
FblI GTMKAC 1 cut(s) 1357
Fnu4HI GCNGC 1 cut(s) 6
FokI GGATG 5 cut(s) 86, 461, 521, 785, 1598
FriOI GRGCYC 1 cut(s) 69
Fsp4HI GCNGC 1 cut(s) 6
FspBI CTAG 2 cut(s) 272, 1722
GluI GCNGC 1 cut(s) 6
GsaI CCCAGC 2 cut(s) 64, 91
GsuI CTGGAG 4 cut(s) 184, 399, 557, 568
HaeIII GGCC 2 cut(s) 35, 86
Hin1II CATG 3 cut(s) 151, 1216, 1370
HincII GTYRAC 1 cut(s) 1358
HindII GTYRAC 1 cut(s) 1358
HindIII AAGCTT 2 cut(s) 12, 1381
HinfI GANTC 6 cut(s) 386, 401, 437, 1218, 1321, 1600
HphI GGTGA 2 cut(s) 1545, 1702
Hpy166II GTNNAC 1 cut(s) 1358
Hpy188III TCNNGA 6 cut(s) 398, 574, 585, 941, 1259, 1604
Hpy8I GTNNAC 1 cut(s) 1358
Hpy99I CGWCG 1 cut(s) 1362
HpyAV CCTTC 6 cut(s) 152, 1093, 1347, 1387, 1645, 1711
HpyCH4III ACNGT 4 cut(s) 173, 355, 634, 1745
HpyCH4IV ACGT 1 cut(s) 1069
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 27, 102, 264, 476
HpyF3I CTNAG 6 cut(s) 372, 423, 564, 965, 1178, 1379
HpySE526I ACGT 1 cut(s) 1069
Hsp92II CATG 3 cut(s) 151, 1216, 1370
Ksp22I TGATCA 2 cut(s) 43, 280
Kzo9I GATC 8 cut(s) 43, 280, 364, 601, 1165, 1174, 1474, 1526
LmnI GCTCC 2 cut(s) 64, 418
LweI GCATC 6 cut(s) 439, 461, 499, 1150, 1337, 1420
MaeI CTAG 2 cut(s) 272, 1722
MaeII ACGT 1 cut(s) 1069
MaeIII GTNAC 1 cut(s) 213
MalI GATC 8 cut(s) 45, 282, 366, 603, 1167, 1176, 1476, 1528
MboI GATC 8 cut(s) 43, 280, 364, 601, 1165, 1174, 1474, 1526
MboII GAAGA 9 cut(s) 446, 562, 688, 1338, 1341, 1560, 1609, 1645, 1682
MfeI CAATTG 1 cut(s) 1518
MflI RGATCY 1 cut(s) 1474
MhlI GDGCHC 1 cut(s) 69
MluCI AATT 6 cut(s) 1286, 1408, 1437, 1449, 1518, 1795
MluI ACGCGT 1 cut(s) 857
MlyI GAGTC 2 cut(s) 395, 1212
MmeI TCCRAC 4 cut(s) 746, 878, 1010, 1142
Mph1103I ATGCAT 2 cut(s) 454, 1435
MroXI GAANNNNTTC 1 cut(s) 141
MseI TTAA 7 cut(s) 54, 593, 1317, 1436, 1482, 1794, 1798
MslI CAYNNNNRTG 2 cut(s) 225, 1425
MspR9I CCNGG 7 cut(s) 755, 821, 887, 953, 1019, 1085, 1151
MunI CAATTG 1 cut(s) 1518
Mva1269I GAATGC 3 cut(s) 318, 1291, 1613
MvaI CCWGG 7 cut(s) 755, 821, 887, 953, 1019, 1085, 1151
MvnI CGCG 1 cut(s) 859
MwoI GCNNNNNNNGC 5 cut(s) 11, 27, 102, 264, 476
NdeII GATC 8 cut(s) 43, 280, 364, 601, 1165, 1174, 1474, 1526
NlaIII CATG 3 cut(s) 151, 1216, 1370
NmeAIII GCCGAG 1 cut(s) 61
NmuCI GTSAC 1 cut(s) 213
NsiI ATGCAT 2 cut(s) 454, 1435
NspI RCATGY 1 cut(s) 1216
PacI TTAATTAA 1 cut(s) 1798
PcsI WCGNNNNNNNCGW 1 cut(s) 23
PctI GAATGC 3 cut(s) 318, 1291, 1613
PdmI GAANNNNTTC 1 cut(s) 141
PfeI GAWTC 4 cut(s) 401, 437, 1321, 1600
PkrI GCNGC 1 cut(s) 7
PleI GAGTC 2 cut(s) 394, 1212
PpsI GAGTC 2 cut(s) 394, 1212
PshBI ATTAAT 1 cut(s) 1436
Psp124BI GAGCTC 1 cut(s) 69
Psp6I CCWGG 7 cut(s) 753, 819, 885, 951, 1017, 1083, 1149
PspFI CCCAGC 2 cut(s) 60, 87
PspGI CCWGG 7 cut(s) 753, 819, 885, 951, 1017, 1083, 1149
PspPI GGNCC 5 cut(s) 85, 719, 983, 1115, 1417
PsuI RGATCY 1 cut(s) 1474
RsaI GTAC 4 cut(s) 338, 655, 1156, 1747
RsaNI GTAC 4 cut(s) 337, 654, 1155, 1746
RseI CAYNNNNRTG 2 cut(s) 225, 1425
SacI GAGCTC 1 cut(s) 69
SalI GTCGAC 1 cut(s) 1356
SaqAI TTAA 7 cut(s) 54, 593, 1317, 1436, 1482, 1794, 1798
SatI GCNGC 1 cut(s) 6
Sau3AI GATC 8 cut(s) 43, 280, 364, 601, 1165, 1174, 1474, 1526
Sau96I GGNCC 5 cut(s) 85, 719, 983, 1115, 1417
ScaI AGTACT 1 cut(s) 1747
SchI GAGTC 2 cut(s) 395, 1212
ScrFI CCNGG 7 cut(s) 755, 821, 887, 953, 1019, 1085, 1151
SduI GDGCHC 1 cut(s) 69
SfaNI GCATC 6 cut(s) 439, 461, 499, 1150, 1337, 1420
SfcI CTRYAG 5 cut(s) 729, 795, 927, 993, 1059
SinI GGWCC 4 cut(s) 719, 983, 1115, 1417
SmiMI CAYNNNNRTG 2 cut(s) 225, 1425
SmlI CTYRAG 2 cut(s) 68, 501
SmoI CTYRAG 2 cut(s) 68, 501
Sse9I AATT 6 cut(s) 1286, 1408, 1437, 1449, 1518, 1795
SsiI CCGC 5 cut(s) 105, 155, 1163, 1402, 1729
SspMI CTAG 2 cut(s) 272, 1722
SstI GAGCTC 1 cut(s) 69
StyD4I CCNGG 7 cut(s) 753, 819, 885, 951, 1017, 1083, 1149
StyI CCWWGG 2 cut(s) 701, 833
TaaI ACNGT 4 cut(s) 173, 355, 634, 1745
TaiI ACGT 1 cut(s) 1072
TaqI TCGA 5 cut(s) 384, 1168, 1258, 1357, 1509
TasI AATT 6 cut(s) 1286, 1408, 1437, 1449, 1518, 1795
TatI WGTACW 3 cut(s) 336, 653, 1745
TfiI GAWTC 4 cut(s) 401, 437, 1321, 1600
Tru1I TTAA 7 cut(s) 54, 593, 1317, 1436, 1482, 1794, 1798
Tru9I TTAA 7 cut(s) 54, 593, 1317, 1436, 1482, 1794, 1798
TscAI CASTG 3 cut(s) 240, 496, 1448
TseFI GTSAC 1 cut(s) 213
TseI GCWGC 1 cut(s) 5
Tsp45I GTSAC 1 cut(s) 213
TspDTI ATGAA 5 cut(s) 348, 858, 1664, 1683, 1759
TspGWI ACGGA 2 cut(s) 461, 1375
TspRI CASTG 3 cut(s) 240, 496, 1448
VpaK11BI GGWCC 4 cut(s) 719, 983, 1115, 1417
VspI ATTAAT 1 cut(s) 1436
XapI RAATTY 2 cut(s) 1408, 1449
XceI RCATGY 1 cut(s) 1216
XcmI CCANNNNNNNNNTGG 1 cut(s) 1321
XmiI GTMKAC 1 cut(s) 1357
XmnI GAANNNNTTC 1 cut(s) 141
XspI CTAG 2 cut(s) 272, 1722
ZrmI AGTACT 1 cut(s) 1747
Zsp2I ATGCAT 2 cut(s) 454, 1435
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.