RLG00000033647

B3 DNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0001196
Physical Location & Seq
Reverse (-)
30170798 .. 30173722
2925 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000033647

Sequence Viewer

Length: 759 bp
ATGGAAGCGGAAGGCTGGAGAGACTGTGCTGTTTGTGGGAAGCCACTCCATTGTGGGTGCATTATGTCACGCCACAAGTATGCACCACTGGATTTCGGAGGTGTAGGTTGCACAAAATGCTTACTAGAAGTTGATCAATCTATTGCTTCAGGTCGGATGACCAGAATGCTGAAAGAAATAGTTGCCAAGTACAAGACGGACCCAACAGTTTCATCTGATCCTACTAAGAAAGTTGTCGAGTCCCTTTTTCCTGAATCTTTACAAGCTCCAGCACCTCAGATTGGAGAAGAATCCAATACGGATGTATCTAAAGGAGATACAGCTTGTGAATTCAAAGTAGGCAGTGAAACACCTCAAGTGGATGCCTCCACAGGAAACCATTTACACCCTGACAATCTGCCGAAGATGACAAGGGCTGAGCAACTCCAAAAAAAGTCATCAACGGTAAGTGATGCTAATCACGGTACAGGATTTCTTGTGATACCGAAGAAATATGCAGAGTCGAATGCCCCTATAGAAACAAATCAGAAAACGAACCAGGAACAATCTCTAAGGGACTTTGAAACGGGTCCAACGCCTGCAGATACAAGTCTGAAAACAGACCAGGAACAACCTCCAAAGGATATGGAGACAAGTCCAATGCCTGTAGAAACAAGTCATAAATCAGACCAGGGACAATCTCCAAAGGATATGAATACAGGTCCAACGCCTGTAGAAACAAGTCTGAAAACAGACCACGTTCAACACCTGCAGAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

253

Amino Acids

27.45

Weight (kDa)

5.52

Isoelectric Point (pI)

48.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf_VAL1_N PF25813 2 - 42 3.3e-19 VAL1-like, N-terminal zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 8
AclWI GGATC 1 cut(s) 212
AcsI RAATTY 1 cut(s) 329
AcuI CTGAAG 1 cut(s) 132
AfaI GTAC 2 cut(s) 191, 466
AfiI CCNNNNNNNGG 1 cut(s) 281
AgsI TTSAA 3 cut(s) 334, 563, 743
AjnI CCWGG 3 cut(s) 537, 603, 669
AluBI AGCT 2 cut(s) 266, 323
AluI AGCT 2 cut(s) 266, 323
Alw26I GTCTC 2 cut(s) 15, 623
AlwI GGATC 1 cut(s) 212
ApoI RAATTY 1 cut(s) 329
AspS9I GGNCC 3 cut(s) 199, 569, 701
AvaII GGWCC 3 cut(s) 199, 569, 701
BaeI ACNNNNGTAYC 2 cut(s) 288, 321
BciT130I CCWGG 3 cut(s) 539, 605, 671
BclI TGATCA 1 cut(s) 133
BcoDI GTCTC 2 cut(s) 15, 623
BfaI CTAG 1 cut(s) 125
BfmI CTRYAG 5 cut(s) 513, 579, 645, 711, 749
BlpI GCTNAGC 1 cut(s) 417
Bme1390I CCNGG 3 cut(s) 539, 605, 671
Bme18I GGWCC 3 cut(s) 199, 569, 701
BmgT120I GGNCC 3 cut(s) 199, 569, 701
BmiI GGNNCC 2 cut(s) 201, 570
BmrFI CCNGG 3 cut(s) 539, 605, 671
BmsI GCATC 2 cut(s) 352, 442
BpmI CTGGAG 2 cut(s) 37, 252
Bpu1102I GCTNAGC 1 cut(s) 417
BpuEI CTTGAG 1 cut(s) 339
BsaBI GATNNNNATC 1 cut(s) 456
BsaJI CCNNGG 1 cut(s) 670
BsaXI ACNNNNNCTCC 2 cut(s) 10, 40
Bsc4I CCNNNNNNNGG 1 cut(s) 281
Bse1I ACTGG 1 cut(s) 93
Bse8I GATNNNNATC 1 cut(s) 456
BseBI CCWGG 3 cut(s) 539, 605, 671
BseDI CCNNGG 1 cut(s) 670
BseGI GGATG 3 cut(s) 162, 307, 367
BseJI GATNNNNATC 1 cut(s) 456
BseLI CCNNNNNNNGG 1 cut(s) 281
BseMII CTCAG 2 cut(s) 290, 408
BseNI ACTGG 1 cut(s) 93
BslFI GGGAC 3 cut(s) 226, 569, 687
BslI CCNNNNNNNGG 1 cut(s) 281
BsmAI GTCTC 2 cut(s) 15, 623
BsmFI GGGAC 3 cut(s) 226, 569, 687
BsmI GAATGC 2 cut(s) 171, 511
Bsp143I GATC 2 cut(s) 133, 217
Bsp1720I GCTNAGC 1 cut(s) 417
BspACI CCGC 1 cut(s) 8
BspCNI CTCAG 2 cut(s) 289, 409
BspLI GGNNCC 2 cut(s) 201, 570
BspMAI CTGCAG 2 cut(s) 583, 753
BspPI GGATC 1 cut(s) 212
BsrI ACTGG 1 cut(s) 93
BssECI CCNNGG 1 cut(s) 670
BssMI GATC 2 cut(s) 133, 217
Bst2UI CCWGG 3 cut(s) 539, 605, 671
Bst4CI ACNGT 4 cut(s) 26, 208, 445, 464
BstAPI GCANNNNNTGC 1 cut(s) 117
BstC8I GCNNGC 1 cut(s) 579
BstDEI CTNAG 4 cut(s) 225, 276, 417, 551
BstF5I GGATG 3 cut(s) 162, 307, 367
BstKTI GATC 2 cut(s) 136, 220
BstMAI GTCTC 2 cut(s) 15, 623
BstMBI GATC 2 cut(s) 133, 217
BstMWI GCNNNNNNNGC 1 cut(s) 117
BstNI CCWGG 3 cut(s) 539, 605, 671
BstSCI CCNGG 3 cut(s) 537, 603, 669
BstSFI CTRYAG 5 cut(s) 513, 579, 645, 711, 749
BtsCI GGATG 3 cut(s) 162, 307, 367
BtsI GCAGTG 1 cut(s) 349
BtsIMutI CAGTG 2 cut(s) 86, 349
Cac8I GCNNGC 1 cut(s) 579
Cfr13I GGNCC 3 cut(s) 199, 569, 701
Csp6I GTAC 2 cut(s) 190, 465
CviJI RGCY 5 cut(s) 15, 43, 266, 323, 416
CviKI_1 RGCY 5 cut(s) 15, 43, 266, 323, 416
CviQI GTAC 2 cut(s) 190, 465
DdeI CTNAG 4 cut(s) 225, 276, 417, 551
DpnI GATC 2 cut(s) 135, 219
DpnII GATC 2 cut(s) 133, 217
Eco47I GGWCC 3 cut(s) 199, 569, 701
Eco57I CTGAAG 1 cut(s) 132
EcoRI GAATTC 1 cut(s) 329
EcoRII CCWGG 3 cut(s) 537, 603, 669
FaiI YATR 7 cut(s) 65, 81, 495, 515, 626, 660, 692
FaqI GGGAC 3 cut(s) 226, 569, 687
FbaI TGATCA 1 cut(s) 133
FokI GGATG 3 cut(s) 169, 314, 374
FspBI CTAG 1 cut(s) 125
GsuI CTGGAG 2 cut(s) 37, 252
HinfI GANTC 4 cut(s) 239, 254, 290, 500
Hpy188I TCNGA 8 cut(s) 98, 156, 217, 279, 528, 594, 667, 726
Hpy188III TCNNGA 1 cut(s) 251
HpyAV CCTTC 1 cut(s) 5
HpyCH4III ACNGT 4 cut(s) 26, 208, 445, 464
HpyCH4IV ACGT 1 cut(s) 738
HpyCH4V TGCA 6 cut(s) 60, 83, 111, 497, 581, 751
HpyF10VI GCNNNNNNNGC 1 cut(s) 117
HpyF3I CTNAG 4 cut(s) 225, 276, 417, 551
HpySE526I ACGT 1 cut(s) 738
Ksp22I TGATCA 1 cut(s) 133
Kzo9I GATC 2 cut(s) 133, 217
LmnI GCTCC 1 cut(s) 271
LweI GCATC 2 cut(s) 352, 442
MaeI CTAG 1 cut(s) 125
MaeII ACGT 1 cut(s) 738
MaeIII GTNAC 1 cut(s) 66
MalI GATC 2 cut(s) 135, 219
MboI GATC 2 cut(s) 133, 217
MboII GAAGA 3 cut(s) 299, 415, 499
MluCI AATT 1 cut(s) 329
MlyI GAGTC 2 cut(s) 248, 509
MmeI TCCRAC 3 cut(s) 134, 596, 728
MnlI CCTC 5 cut(s) 92, 285, 363, 376, 624
MslI CAYNNNNRTG 1 cut(s) 78
MspR9I CCNGG 3 cut(s) 539, 605, 671
Mva1269I GAATGC 2 cut(s) 171, 511
MvaI CCWGG 3 cut(s) 539, 605, 671
MwoI GCNNNNNNNGC 1 cut(s) 117
NdeII GATC 2 cut(s) 133, 217
NlaIV GGNNCC 2 cut(s) 201, 570
NmuCI GTSAC 1 cut(s) 66
PctI GAATGC 2 cut(s) 171, 511
PfeI GAWTC 2 cut(s) 254, 290
PleI GAGTC 2 cut(s) 247, 508
PpsI GAGTC 2 cut(s) 247, 508
Psp6I CCWGG 3 cut(s) 537, 603, 669
PspGI CCWGG 3 cut(s) 537, 603, 669
PspN4I GGNNCC 2 cut(s) 201, 570
PspPI GGNCC 3 cut(s) 199, 569, 701
PstI CTGCAG 2 cut(s) 583, 753
RsaI GTAC 2 cut(s) 191, 466
RsaNI GTAC 2 cut(s) 190, 465
RseI CAYNNNNRTG 1 cut(s) 78
Sau3AI GATC 2 cut(s) 133, 217
Sau96I GGNCC 3 cut(s) 199, 569, 701
SchI GAGTC 2 cut(s) 248, 509
ScrFI CCNGG 3 cut(s) 539, 605, 671
SfaNI GCATC 2 cut(s) 352, 442
SfcI CTRYAG 5 cut(s) 513, 579, 645, 711, 749
SinI GGWCC 3 cut(s) 199, 569, 701
SmiMI CAYNNNNRTG 1 cut(s) 78
SmlI CTYRAG 1 cut(s) 354
SmoI CTYRAG 1 cut(s) 354
Sse9I AATT 1 cut(s) 329
SsiI CCGC 1 cut(s) 8
SspMI CTAG 1 cut(s) 125
StyD4I CCNGG 3 cut(s) 537, 603, 669
TaaI ACNGT 4 cut(s) 26, 208, 445, 464
TaiI ACGT 1 cut(s) 741
TaqI TCGA 2 cut(s) 237, 503
TasI AATT 1 cut(s) 329
TatI WGTACW 1 cut(s) 189
TfiI GAWTC 2 cut(s) 254, 290
TscAI CASTG 2 cut(s) 93, 349
TseFI GTSAC 1 cut(s) 66
Tsp45I GTSAC 1 cut(s) 66
TspDTI ATGAA 2 cut(s) 201, 707
TspGWI ACGGA 2 cut(s) 212, 314
TspRI CASTG 2 cut(s) 93, 349
VpaK11BI GGWCC 3 cut(s) 199, 569, 701
XapI RAATTY 1 cut(s) 329
XspI CTAG 1 cut(s) 125
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.