Rh5DG253300

B3 DNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0001196
Physical Location & Seq
Reverse (-)
29242893 .. 29248801
5909 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG253300.1

Sequence Viewer

Length: 876 bp
ATGGCTGGGAGCTCAAGGATGGGTCTTTCGCCTAGCTCTGCAAGAAATGCGGGCTGGAGAGACTGTGCTGTTTGTGGGAAGCCACTCCATTGTGGGTGCATTATGTCATGCAACAAGTATGCACCACTGGATTTCGGAGGTGTAGGTTGCTCAAAATGCTTACTAGAAGTTGATCAGTCTATTGCTTCAGGTCAAATGACCAGAATGCTGAAAGAAATAGTTGCCACGTATAAGACAGGTCCAACAGTTTCATCTGATCCTACTGAGAAAGTTGTCGAGTCCCTTTCTCCTGGATCTTCACAAGCGCCTGCTCCTCATTTTGGAGAAGAAGCCAATACGGATGCATCTAAAGGAGATACAGCTTGTGAAACCAAAGTAGACAGTGAAACACCTCAAGTGGATATCTCCACAGGAAACCATTTACACCCTGACAATCTGCCGAAGATGACAAATGCTGAGCAACTCCAAAAAGTCTCCGGAAACTTAAACTCTGATCTTATACCTCTGTTTGAGAAGTCATCAACCACAAGTGATGCTAATCGTAGTACAGGATGTCTTGTGATACCAAAGAAATACGCAGAGGAACACTCTCCAAGGGACATTGAAACAAGTCCAACACCTGTAGATACAAGTCTGAAAACAGACCAGGAACAAACTCCAAGGGACATTGAAACAGGTCCAACGCCTGTAGATACAAGTGGACAATCTCCAAGGGATATGGAGACAAGTCCAACTTTTGTAGAAGCAAGTCAGAAATCAGACAAGGAACAATCTCAAAGGGATATGGAAACAGGTCCAACACCTGCAGAAACAATCCTTAAAACAGACCAGGAACAGTCTCCGAGGGGTATGGAAACAAGTCTGAAAACAGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

291

Amino Acids

31.12

Weight (kDa)

4.8

Isoelectric Point (pI)

51.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf_VAL1_N PF25813 15 - 55 9e-18 VAL1-like, N-terminal zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 811
Acc36I ACCTGC 1 cut(s) 811
AccI GTMKAC 1 cut(s) 378
AccIII TCCGGA 1 cut(s) 476
AciI CCGC 1 cut(s) 50
AclWI GGATC 2 cut(s) 251, 301
AcuI CTGAAG 1 cut(s) 171
AfaI GTAC 1 cut(s) 547
AfiI CCNNNNNNNGG 1 cut(s) 320
AgsI TTSAA 2 cut(s) 605, 671
AjnI CCWGG 3 cut(s) 289, 645, 828
AluBI AGCT 3 cut(s) 12, 36, 362
AluI AGCT 3 cut(s) 12, 36, 362
Alw21I GWGCWC 1 cut(s) 14
Alw26I GTCTC 4 cut(s) 54, 478, 716, 843
AlwI GGATC 2 cut(s) 251, 301
Aor13HI TCCGGA 1 cut(s) 476
AspLEI GCGC 1 cut(s) 307
AspS9I GGNCC 3 cut(s) 239, 677, 794
AvaII GGWCC 3 cut(s) 239, 677, 794
BanII GRGCYC 1 cut(s) 14
Bbv12I GWGCWC 1 cut(s) 14
BccI CCATC 1 cut(s) 13
BciT130I CCWGG 3 cut(s) 291, 647, 830
BclI TGATCA 1 cut(s) 172
BcoDI GTCTC 4 cut(s) 54, 478, 716, 843
BfaI CTAG 3 cut(s) 33, 164, 874
BfmI CTRYAG 3 cut(s) 621, 687, 804
BfoI RGCGCY 1 cut(s) 308
BfuAI ACCTGC 1 cut(s) 811
BlpI GCTNAGC 1 cut(s) 456
Bme1390I CCNGG 3 cut(s) 291, 647, 830
Bme18I GGWCC 3 cut(s) 239, 677, 794
BmgT120I GGNCC 3 cut(s) 239, 677, 794
BmrFI CCNGG 3 cut(s) 291, 647, 830
BmsI GCATC 3 cut(s) 331, 353, 523
BplI GAGNNNNNCTC 2 cut(s) 572, 604
BpmI CTGGAG 1 cut(s) 76
Bpu1102I GCTNAGC 1 cut(s) 456
BpuEI CTTGAG 1 cut(s) 378
BsaAI YACGTR 1 cut(s) 228
BsaBI GATNNNNATC 1 cut(s) 537
BsaJI CCNNGG 4 cut(s) 593, 659, 710, 842
BsaWI WCCGGW 1 cut(s) 476
BsaXI ACNNNNNCTCC 2 cut(s) 49, 79
Bsc4I CCNNNNNNNGG 1 cut(s) 320
Bse1I ACTGG 1 cut(s) 132
Bse8I GATNNNNATC 1 cut(s) 537
BseAI TCCGGA 1 cut(s) 476
BseBI CCWGG 3 cut(s) 291, 647, 830
BseDI CCNNGG 4 cut(s) 593, 659, 710, 842
BseGI GGATG 3 cut(s) 24, 346, 557
BseJI GATNNNNATC 1 cut(s) 537
BseLI CCNNNNNNNGG 1 cut(s) 320
BseMII CTCAG 2 cut(s) 255, 447
BseNI ACTGG 1 cut(s) 132
BseRI GAGGAG 1 cut(s) 303
BseYI CCCAGC 1 cut(s) 5
BsiHKAI GWGCWC 1 cut(s) 14
BsiSI CCGG 1 cut(s) 477
BslFI GGGAC 3 cut(s) 265, 611, 677
BslI CCNNNNNNNGG 1 cut(s) 320
BsmAI GTCTC 4 cut(s) 54, 478, 716, 843
BsmFI GGGAC 3 cut(s) 265, 611, 677
BsmI GAATGC 1 cut(s) 210
Bsp1286I GDGCHC 1 cut(s) 14
Bsp13I TCCGGA 1 cut(s) 476
Bsp143I GATC 4 cut(s) 172, 256, 293, 493
Bsp1720I GCTNAGC 1 cut(s) 456
BspACI CCGC 1 cut(s) 50
BspCNI CTCAG 2 cut(s) 256, 448
BspEI TCCGGA 1 cut(s) 476
BspMAI CTGCAG 1 cut(s) 808
BspMI ACCTGC 1 cut(s) 811
BspPI GGATC 2 cut(s) 251, 301
BsrI ACTGG 1 cut(s) 132
BssECI CCNNGG 4 cut(s) 593, 659, 710, 842
BssMI GATC 4 cut(s) 172, 256, 293, 493
BssT1I CCWWGG 3 cut(s) 593, 659, 710
Bst2UI CCWGG 3 cut(s) 291, 647, 830
Bst4CI ACNGT 4 cut(s) 65, 247, 383, 837
BstAPI GCANNNNNTGC 1 cut(s) 47
BstBAI YACGTR 1 cut(s) 228
BstC8I GCNNGC 2 cut(s) 52, 309
BstDEI CTNAG 2 cut(s) 264, 456
BstF5I GGATG 3 cut(s) 24, 346, 557
BstH2I RGCGCY 1 cut(s) 308
BstHHI GCGC 1 cut(s) 307
BstKTI GATC 4 cut(s) 175, 259, 296, 496
BstMAI GTCTC 4 cut(s) 54, 478, 716, 843
BstMBI GATC 4 cut(s) 172, 256, 293, 493
BstMWI GCNNNNNNNGC 2 cut(s) 47, 156
BstNI CCWGG 3 cut(s) 291, 647, 830
BstSCI CCNGG 3 cut(s) 289, 645, 828
BstSFI CTRYAG 3 cut(s) 621, 687, 804
BstX2I RGATCY 1 cut(s) 293
BstYI RGATCY 1 cut(s) 293
BtsCI GGATG 3 cut(s) 24, 346, 557
BtsIMutI CAGTG 2 cut(s) 125, 388
BveI ACCTGC 1 cut(s) 811
Cac8I GCNNGC 2 cut(s) 52, 309
CfoI GCGC 1 cut(s) 307
Cfr13I GGNCC 3 cut(s) 239, 677, 794
Csp6I GTAC 1 cut(s) 546
CviAII CATG 1 cut(s) 108
CviJI RGCY 7 cut(s) 5, 12, 36, 54, 82, 332, 362
CviKI_1 RGCY 7 cut(s) 5, 12, 36, 54, 82, 332, 362
CviQI GTAC 1 cut(s) 546
DdeI CTNAG 2 cut(s) 264, 456
DpnI GATC 4 cut(s) 174, 258, 295, 495
DpnII GATC 4 cut(s) 172, 256, 293, 493
Ecl136II GAGCTC 1 cut(s) 12
Eco130I CCWWGG 3 cut(s) 593, 659, 710
Eco24I GRGCYC 1 cut(s) 14
Eco32I GATATC 1 cut(s) 403
Eco47I GGWCC 3 cut(s) 239, 677, 794
Eco53kI GAGCTC 1 cut(s) 12
Eco57I CTGAAG 1 cut(s) 171
EcoICRI GAGCTC 1 cut(s) 12
EcoRII CCWGG 3 cut(s) 289, 645, 828
EcoRV GATATC 1 cut(s) 403
EcoT14I CCWWGG 3 cut(s) 593, 659, 710
EcoT22I ATGCAT 1 cut(s) 346
EcoT38I GRGCYC 1 cut(s) 14
ErhI CCWWGG 3 cut(s) 593, 659, 710
FaeI CATG 1 cut(s) 111
FaiI YATR 8 cut(s) 104, 109, 120, 231, 500, 719, 785, 851
FalI AAGNNNNNCTT 2 cut(s) 718, 750
FaqI GGGAC 3 cut(s) 265, 611, 677
FatI CATG 1 cut(s) 107
FauI CCCGC 1 cut(s) 43
FbaI TGATCA 1 cut(s) 172
FblI GTMKAC 1 cut(s) 378
FokI GGATG 3 cut(s) 31, 353, 564
FriOI GRGCYC 1 cut(s) 14
FspBI CTAG 3 cut(s) 33, 164, 874
GlaI GCGC 1 cut(s) 306
GsaI CCCAGC 1 cut(s) 9
GsuI CTGGAG 1 cut(s) 76
HaeII RGCGCY 1 cut(s) 308
HapII CCGG 1 cut(s) 477
HhaI GCGC 1 cut(s) 307
Hin1II CATG 1 cut(s) 111
Hin6I GCGC 1 cut(s) 305
HinP1I GCGC 1 cut(s) 305
HinfI GANTC 1 cut(s) 278
HpaII CCGG 1 cut(s) 477
Hpy166II GTNNAC 2 cut(s) 379, 701
Hpy188I TCNGA 8 cut(s) 137, 256, 493, 636, 753, 760, 843, 864
Hpy188III TCNNGA 1 cut(s) 477
Hpy8I GTNNAC 2 cut(s) 379, 701
HpyCH4III ACNGT 4 cut(s) 65, 247, 383, 837
HpyCH4IV ACGT 1 cut(s) 227
HpyCH4V TGCA 6 cut(s) 41, 99, 111, 122, 344, 806
HpyF10VI GCNNNNNNNGC 2 cut(s) 47, 156
HpyF3I CTNAG 2 cut(s) 264, 456
HpySE526I ACGT 1 cut(s) 227
Hsp92II CATG 1 cut(s) 111
HspAI GCGC 1 cut(s) 305
Kpn2I TCCGGA 1 cut(s) 476
Ksp22I TGATCA 1 cut(s) 172
Kzo9I GATC 4 cut(s) 172, 256, 293, 493
LmnI GCTCC 2 cut(s) 9, 316
LweI GCATC 3 cut(s) 331, 353, 523
MaeI CTAG 3 cut(s) 33, 164, 874
MaeII ACGT 1 cut(s) 227
MalI GATC 4 cut(s) 174, 258, 295, 495
MboI GATC 4 cut(s) 172, 256, 293, 493
MboII GAAGA 3 cut(s) 288, 338, 454
MflI RGATCY 1 cut(s) 293
MhlI GDGCHC 1 cut(s) 14
MlyI GAGTC 1 cut(s) 287
MmeI TCCRAC 5 cut(s) 266, 638, 704, 755, 821
MnlI CCTC 6 cut(s) 131, 324, 402, 513, 574, 837
Mph1103I ATGCAT 1 cut(s) 346
MroI TCCGGA 1 cut(s) 476
MseI TTAA 2 cut(s) 485, 819
MspI CCGG 1 cut(s) 477
MspR9I CCNGG 3 cut(s) 291, 647, 830
Mva1269I GAATGC 1 cut(s) 210
MvaI CCWGG 3 cut(s) 291, 647, 830
MwoI GCNNNNNNNGC 2 cut(s) 47, 156
NdeII GATC 4 cut(s) 172, 256, 293, 493
NlaIII CATG 1 cut(s) 111
NsiI ATGCAT 1 cut(s) 346
PaqCI CACCTGC 1 cut(s) 811
PctI GAATGC 1 cut(s) 210
PfoI TCCNGGA 1 cut(s) 289
PleI GAGTC 1 cut(s) 286
PpsI GAGTC 1 cut(s) 286
Ppu21I YACGTR 1 cut(s) 228
Psp124BI GAGCTC 1 cut(s) 14
Psp6I CCWGG 3 cut(s) 289, 645, 828
PspFI CCCAGC 1 cut(s) 5
PspGI CCWGG 3 cut(s) 289, 645, 828
PspPI GGNCC 3 cut(s) 239, 677, 794
PstI CTGCAG 1 cut(s) 808
PsuI RGATCY 1 cut(s) 293
RsaI GTAC 1 cut(s) 547
RsaNI GTAC 1 cut(s) 546
SacI GAGCTC 1 cut(s) 14
SaqAI TTAA 2 cut(s) 485, 819
Sau3AI GATC 4 cut(s) 172, 256, 293, 493
Sau96I GGNCC 3 cut(s) 239, 677, 794
SchI GAGTC 1 cut(s) 287
ScrFI CCNGG 3 cut(s) 291, 647, 830
SduI GDGCHC 1 cut(s) 14
SfaNI GCATC 3 cut(s) 331, 353, 523
SfcI CTRYAG 3 cut(s) 621, 687, 804
SinI GGWCC 3 cut(s) 239, 677, 794
SmlI CTYRAG 2 cut(s) 13, 393
SmoI CTYRAG 2 cut(s) 13, 393
SsiI CCGC 1 cut(s) 50
SspMI CTAG 3 cut(s) 33, 164, 874
SstI GAGCTC 1 cut(s) 14
StyD4I CCNGG 3 cut(s) 289, 645, 828
StyI CCWWGG 3 cut(s) 593, 659, 710
TaaI ACNGT 4 cut(s) 65, 247, 383, 837
TaiI ACGT 1 cut(s) 230
TaqI TCGA 1 cut(s) 276
TatI WGTACW 1 cut(s) 545
Tru1I TTAA 2 cut(s) 485, 819
Tru9I TTAA 2 cut(s) 485, 819
TscAI CASTG 2 cut(s) 132, 388
TspDTI ATGAA 1 cut(s) 240
TspGWI ACGGA 1 cut(s) 353
TspRI CASTG 2 cut(s) 132, 388
VpaK11BI GGWCC 3 cut(s) 239, 677, 794
XmiI GTMKAC 1 cut(s) 378
XspI CTAG 3 cut(s) 33, 164, 874
Zsp2I ATGCAT 1 cut(s) 346
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.