RchiOBHm_Chr5g0035901

B3 DNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0001196
Physical Location & Seq
Reverse (-)
29955883 .. 29962943
7061 bp
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UTR
Exon/CDS
Intron
PRQ31474

Sequence Viewer

Length: 678 bp
ATGGCTGCCAAAGCTTCGTGCTTTTTCTGCAAGGCCGAGAGTGATCAGTTCGTTAATGGCTGGGAGCTCAAGGATGGGTCTTTCGCCCAGCTCTGCAAGAAATGCGGTTTTGCTTATGTGAATGGTATATTTTGTGAAACCTTTCACATGGAAGCAGAAGGCTGGAGAGACTGTGCTGTTTGTGGGAAGCCACTCCATTGTGGGTGCATTATGTCACGCCACAAGTATGCACCACTGGATTTCGGAGGCGTAGGTTGCACAAAATGCTTACTAGAAGTTGATCAATCTATTGCTTCAGGTCGAATGACCAGAATGCTGAAAGAAATAGTTGCCAAGTACAAGATGGACCCAACAGTTTCATCTGATCCTACTAAGAAAGTTTTTGAGTCCCTTTTTCCTGAATCTTTACAAGCTCCAGCACCTCAGATTGGAGAAGAATCCAATACGGATGCATCTAAAGGAGATACAGCTTGTGAAGCCAAAGTAGGCACTGAAATACCTCAAGTGGATGCCTCCACAGGAAACCATTTACACCCTGACAATCTGCCGAAGATGACAAGGGCTGAGCAACTCCAAAAAGTCTCCAGAAACTTAAACTCTGATCTTATACCTCTGTTTGAGAAGTCATCAACCGTAAGTGATACTAATCACAGTACAGGATTTCTTGTGATACCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

225

Amino Acids

24.57

Weight (kDa)

5.88

Isoelectric Point (pI)

37.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf_VAL1_N PF25813 47 - 91 8.4e-22 VAL1-like, N-terminal zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 105
AclWI GGATC 1 cut(s) 359
AcuI CTGAAG 1 cut(s) 279
AfaI GTAC 2 cut(s) 338, 655
AfiI CCNNNNNNNGG 1 cut(s) 428
AluBI AGCT 5 cut(s) 14, 67, 91, 413, 470
AluI AGCT 5 cut(s) 14, 67, 91, 413, 470
Alw21I GWGCWC 1 cut(s) 69
Alw26I GTCTC 2 cut(s) 162, 586
AlwI GGATC 1 cut(s) 359
AoxI GGCC 1 cut(s) 33
ApeKI GCWGC 1 cut(s) 5
Asp700I GAANNNNTTC 1 cut(s) 141
AspS9I GGNCC 1 cut(s) 346
AvaII GGWCC 1 cut(s) 346
BanII GRGCYC 1 cut(s) 69
Bbv12I GWGCWC 1 cut(s) 69
BccI CCATC 2 cut(s) 68, 337
BclI TGATCA 2 cut(s) 43, 280
BcoDI GTCTC 2 cut(s) 162, 586
BfaI CTAG 1 cut(s) 272
BisI GCNGC 1 cut(s) 6
BlpI GCTNAGC 1 cut(s) 564
BlsI GCNGC 1 cut(s) 7
Bme18I GGWCC 1 cut(s) 346
BmgT120I GGNCC 1 cut(s) 346
BmiI GGNNCC 1 cut(s) 348
BmsI GCATC 3 cut(s) 439, 461, 499
BpmI CTGGAG 3 cut(s) 184, 399, 568
Bpu1102I GCTNAGC 1 cut(s) 564
BpuEI CTTGAG 2 cut(s) 53, 486
BsaBI GATNNNNATC 1 cut(s) 645
BsaXI ACNNNNNCTCC 2 cut(s) 157, 187
Bsc4I CCNNNNNNNGG 1 cut(s) 428
Bse1I ACTGG 1 cut(s) 240
Bse8I GATNNNNATC 1 cut(s) 645
BseGI GGATG 3 cut(s) 79, 454, 514
BseJI GATNNNNATC 1 cut(s) 645
BseLI CCNNNNNNNGG 1 cut(s) 428
BseMII CTCAG 2 cut(s) 437, 555
BseNI ACTGG 1 cut(s) 240
BseYI CCCAGC 2 cut(s) 60, 87
BshFI GGCC 1 cut(s) 35
BsiHKAI GWGCWC 1 cut(s) 69
BslFI GGGAC 1 cut(s) 373
BslI CCNNNNNNNGG 1 cut(s) 428
BsmAI GTCTC 2 cut(s) 162, 586
BsmFI GGGAC 1 cut(s) 373
BsmI GAATGC 1 cut(s) 318
BsnI GGCC 1 cut(s) 35
Bsp1286I GDGCHC 1 cut(s) 69
Bsp143I GATC 4 cut(s) 43, 280, 364, 601
Bsp1720I GCTNAGC 1 cut(s) 564
BspACI CCGC 1 cut(s) 105
BspANI GGCC 1 cut(s) 35
BspCNI CTCAG 2 cut(s) 436, 556
BspLI GGNNCC 1 cut(s) 348
BspPI GGATC 1 cut(s) 359
BsrI ACTGG 1 cut(s) 240
BssMI GATC 4 cut(s) 43, 280, 364, 601
Bst4CI ACNGT 5 cut(s) 173, 355, 634, 653, 675
BstAPI GCANNNNNTGC 2 cut(s) 102, 264
BstDEI CTNAG 3 cut(s) 372, 423, 564
BstF5I GGATG 3 cut(s) 79, 454, 514
BstKTI GATC 4 cut(s) 46, 283, 367, 604
BstMAI GTCTC 2 cut(s) 162, 586
BstMBI GATC 4 cut(s) 43, 280, 364, 601
BstMWI GCNNNNNNNGC 6 cut(s) 11, 27, 102, 255, 264, 476
BsuRI GGCC 1 cut(s) 35
BtsCI GGATG 3 cut(s) 79, 454, 514
BtsIMutI CAGTG 2 cut(s) 233, 489
Cfr13I GGNCC 1 cut(s) 346
Csp6I GTAC 2 cut(s) 337, 654
CviAII CATG 1 cut(s) 148
CviQI GTAC 2 cut(s) 337, 654
DdeI CTNAG 3 cut(s) 372, 423, 564
DpnI GATC 4 cut(s) 45, 282, 366, 603
DpnII GATC 4 cut(s) 43, 280, 364, 601
Ecl136II GAGCTC 1 cut(s) 67
Eco24I GRGCYC 1 cut(s) 69
Eco47I GGWCC 1 cut(s) 346
Eco53kI GAGCTC 1 cut(s) 67
Eco57I CTGAAG 1 cut(s) 279
EcoICRI GAGCTC 1 cut(s) 67
EcoT22I ATGCAT 1 cut(s) 454
EcoT38I GRGCYC 1 cut(s) 69
FaeI CATG 1 cut(s) 151
FaiI YATR 6 cut(s) 117, 128, 149, 212, 228, 608
FaqI GGGAC 1 cut(s) 373
FatI CATG 1 cut(s) 147
FbaI TGATCA 2 cut(s) 43, 280
Fnu4HI GCNGC 1 cut(s) 6
FokI GGATG 3 cut(s) 86, 461, 521
FriOI GRGCYC 1 cut(s) 69
Fsp4HI GCNGC 1 cut(s) 6
FspBI CTAG 1 cut(s) 272
GluI GCNGC 1 cut(s) 6
GsaI CCCAGC 2 cut(s) 64, 91
GsuI CTGGAG 3 cut(s) 184, 399, 568
HaeIII GGCC 1 cut(s) 35
Hin1II CATG 1 cut(s) 151
HindIII AAGCTT 1 cut(s) 12
HinfI GANTC 3 cut(s) 386, 401, 437
Hpy188I TCNGA 4 cut(s) 245, 364, 426, 601
Hpy188III TCNNGA 2 cut(s) 398, 585
HpyAV CCTTC 1 cut(s) 152
HpyCH4III ACNGT 5 cut(s) 173, 355, 634, 653, 675
HpyCH4V TGCA 6 cut(s) 30, 96, 207, 230, 258, 452
HpyF10VI GCNNNNNNNGC 6 cut(s) 11, 27, 102, 255, 264, 476
HpyF3I CTNAG 3 cut(s) 372, 423, 564
Hsp92II CATG 1 cut(s) 151
Ksp22I TGATCA 2 cut(s) 43, 280
Kzo9I GATC 4 cut(s) 43, 280, 364, 601
LmnI GCTCC 2 cut(s) 64, 418
LweI GCATC 3 cut(s) 439, 461, 499
MaeI CTAG 1 cut(s) 272
MaeIII GTNAC 1 cut(s) 213
MalI GATC 4 cut(s) 45, 282, 366, 603
MboI GATC 4 cut(s) 43, 280, 364, 601
MboII GAAGA 2 cut(s) 446, 562
MhlI GDGCHC 1 cut(s) 69
MlyI GAGTC 1 cut(s) 395
MnlI CCTC 5 cut(s) 239, 432, 510, 523, 621
Mph1103I ATGCAT 1 cut(s) 454
MroXI GAANNNNTTC 1 cut(s) 141
MseI TTAA 2 cut(s) 54, 593
MslI CAYNNNNRTG 1 cut(s) 225
Mva1269I GAATGC 1 cut(s) 318
MwoI GCNNNNNNNGC 6 cut(s) 11, 27, 102, 255, 264, 476
NdeII GATC 4 cut(s) 43, 280, 364, 601
NlaIII CATG 1 cut(s) 151
NlaIV GGNNCC 1 cut(s) 348
NmeAIII GCCGAG 1 cut(s) 61
NmuCI GTSAC 1 cut(s) 213
NsiI ATGCAT 1 cut(s) 454
PctI GAATGC 1 cut(s) 318
PdmI GAANNNNTTC 1 cut(s) 141
PfeI GAWTC 2 cut(s) 401, 437
PkrI GCNGC 1 cut(s) 7
PleI GAGTC 1 cut(s) 394
PpsI GAGTC 1 cut(s) 394
Psp124BI GAGCTC 1 cut(s) 69
PspFI CCCAGC 2 cut(s) 60, 87
PspN4I GGNNCC 1 cut(s) 348
PspPI GGNCC 1 cut(s) 346
RsaI GTAC 2 cut(s) 338, 655
RsaNI GTAC 2 cut(s) 337, 654
RseI CAYNNNNRTG 1 cut(s) 225
SacI GAGCTC 1 cut(s) 69
SaqAI TTAA 2 cut(s) 54, 593
SatI GCNGC 1 cut(s) 6
Sau3AI GATC 4 cut(s) 43, 280, 364, 601
Sau96I GGNCC 1 cut(s) 346
SchI GAGTC 1 cut(s) 395
SduI GDGCHC 1 cut(s) 69
SfaNI GCATC 3 cut(s) 439, 461, 499
SinI GGWCC 1 cut(s) 346
SmiMI CAYNNNNRTG 1 cut(s) 225
SmlI CTYRAG 2 cut(s) 68, 501
SmoI CTYRAG 2 cut(s) 68, 501
SsiI CCGC 1 cut(s) 105
SspMI CTAG 1 cut(s) 272
SstI GAGCTC 1 cut(s) 69
TaaI ACNGT 5 cut(s) 173, 355, 634, 653, 675
TaqI TCGA 1 cut(s) 301
TatI WGTACW 2 cut(s) 336, 653
TfiI GAWTC 2 cut(s) 401, 437
Tru1I TTAA 2 cut(s) 54, 593
Tru9I TTAA 2 cut(s) 54, 593
TscAI CASTG 2 cut(s) 240, 496
TseFI GTSAC 1 cut(s) 213
TseI GCWGC 1 cut(s) 5
Tsp45I GTSAC 1 cut(s) 213
TspDTI ATGAA 1 cut(s) 348
TspGWI ACGGA 1 cut(s) 461
TspRI CASTG 2 cut(s) 240, 496
VpaK11BI GGWCC 1 cut(s) 346
XcmI CCANNNNNNNNNTGG 1 cut(s) 340
XmnI GAANNNNTTC 1 cut(s) 141
XspI CTAG 1 cut(s) 272
Zsp2I ATGCAT 1 cut(s) 454
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.