Prupe.4G190000_v2.0.a1

B3 DNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0001196
Physical Location & Seq
Reverse (-)
11300877 .. 11316907
16031 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G190000.4

Sequence Viewer

Length: 1098 bp
ATGGCTCTGACTCACACTCGCTCACTCGTATATATACTGGATGTCCTTATCTTTCAACACTTTCTCTGCCCCTCTCTCTCTCGTTTGCTCTCTCTGTTCTCTCTGTCTCGTTTGCTCTCTCTGTTTCTCTCTGTCTCATTTGCTCTCTCATTCTCTCTCACTCTTTGTGTTGCTTCAATGGCTTCAATGGCTTCCGAGCACTGCTTCTTCTGCAAGGCTGAGATTGCTGAGTTCATTACTGGCTGGCCGCTGAAAGATGGCTCTTTCGCTAAGCTCTGCGGGAAATGCGGTTATGCTTATATACATGATCAGTTTTGTGAAACTTTTCATGCCAAGTCTGATGGATGGAGACACTGTGATGGCTGCAAGAAGCGACTCCACTGTGGATGCATTATGTCATGCCACACGTACACGGAACTGGATTATGGAGGTGTAGGTTGCAGTAAATGCTTATTGCGAAGTAAAAAATCTATGGAGTCAGCTGCCAATGAAGGGACGTATTTAACAACATTATCTGATTCTGATACCCATTCTGTGGTATCTAATGAGATAATTGCTGAATGCTGTTCTCCTGGATCATCTAAAGGAAAAGCTTCTGCACTTATTGGAGAAAACTCTAATGTGGATTCTAGTGGACATACAGGTTGTGAACAAGGAGTAGAGAGTGAAACGCCACGAGTGGATGCCTCTACTCAAAACCATTTATCGAACATTGATGCTATACCTCTGTTTGAGAAGTCATTAACCGCAAGTGATACTTTCCTGAATACAGCACGTCTAGTGATACCAAAGAAATGTGCAGAGGCCCATTTTCCAAAAATTTCTGAAACACAAGGATTCCCCATCGTAATTCAGGATTACACAGGCAGGGACTGGAAATTTCAATATCGTTGCTGGCAAAATAGTAACAGCAAAAAGATGTATGTATTAGAAGGGCTCAAGGATTACATGATCTTAAATCAGTGGCAAGTTGGGGACACAGTGACGTTTCATCGAACAGAACAAGAAGGAAAACTGATTATTGGAACAAAAAAGATGCCAGCTTCTATTGCTTCTATTCAAGCTCTTAAGGAAAAAATTTGGAAACTACTAGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

366

Amino Acids

40.46

Weight (kDa)

7.48

Isoelectric Point (pI)

32.81

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 535
AciI CCGC 4 cut(s) 248, 279, 288, 747
AclWI GGATC 1 cut(s) 583
AcoI YGGCCR 1 cut(s) 245
AcsI RAATTY 3 cut(s) 819, 878, 1077
AfaI GTAC 1 cut(s) 410
AfiI CCNNNNNNNGG 2 cut(s) 492, 535
AflII CTTAAG 1 cut(s) 1067
AflIII ACRYGT 1 cut(s) 405
AgsI TTSAA 5 cut(s) 56, 177, 186, 884, 1061
AjiI CACGTC 1 cut(s) 776
AjnI CCWGG 1 cut(s) 571
AluBI AGCT 5 cut(s) 274, 482, 593, 1043, 1064
AluI AGCT 5 cut(s) 274, 482, 593, 1043, 1064
Alw21I GWGCWC 1 cut(s) 201
Alw26I GTCTC 3 cut(s) 111, 139, 343
AlwI GGATC 1 cut(s) 583
AlwNI CAGNNNCTG 1 cut(s) 873
AoxI GGCC 2 cut(s) 245, 804
ApeKI GCWGC 2 cut(s) 363, 482
ApoI RAATTY 3 cut(s) 819, 878, 1077
Asp700I GAANNNNTTC 2 cut(s) 324, 592
AspS9I GGNCC 1 cut(s) 805
BanII GRGCYC 1 cut(s) 939
BauI CACGAG 1 cut(s) 675
Bbv12I GWGCWC 1 cut(s) 201
BbvI GCAGC 2 cut(s) 350, 469
BccI CCATC 5 cut(s) 251, 335, 339, 353, 851
BciT130I CCWGG 1 cut(s) 573
BclI TGATCA 1 cut(s) 307
BcoDI GTCTC 3 cut(s) 111, 139, 343
BfaI CTAG 3 cut(s) 630, 779, 1091
BfrI CTTAAG 1 cut(s) 1067
BisI GCNGC 3 cut(s) 248, 364, 483
BlpI GCTNAGC 1 cut(s) 270
BlsI GCNGC 3 cut(s) 249, 365, 484
Bme1390I CCNGG 1 cut(s) 573
BmgBI CACGTC 1 cut(s) 776
BmgT120I GGNCC 1 cut(s) 805
BmrFI CCNGG 1 cut(s) 573
BmsI GCATC 4 cut(s) 377, 673, 706, 1026
Bpu1102I GCTNAGC 1 cut(s) 270
BpuEI CTTGAG 1 cut(s) 923
BsaAI YACGTR 1 cut(s) 408
BsaXI ACNNNNNCTCC 2 cut(s) 340, 370
Bsc4I CCNNNNNNNGG 2 cut(s) 492, 535
Bse1I ACTGG 4 cut(s) 42, 244, 423, 878
BseBI CCWGG 1 cut(s) 573
BseGI GGATG 4 cut(s) 46, 350, 392, 688
BseLI CCNNNNNNNGG 2 cut(s) 492, 535
BseMII CTCAG 2 cut(s) 210, 219
BseNI ACTGG 4 cut(s) 42, 244, 423, 878
BseXI GCAGC 2 cut(s) 350, 469
BsgI GTGCAG 2 cut(s) 582, 819
BshFI GGCC 2 cut(s) 247, 806
BsiHKAI GWGCWC 1 cut(s) 201
BslFI GGGAC 3 cut(s) 508, 884, 989
BslI CCNNNNNNNGG 2 cut(s) 492, 535
BsmAI GTCTC 3 cut(s) 111, 139, 343
BsmFI GGGAC 3 cut(s) 508, 884, 989
BsmI GAATGC 1 cut(s) 566
BsnI GGCC 2 cut(s) 247, 806
Bsp1286I GDGCHC 2 cut(s) 201, 939
Bsp143I GATC 3 cut(s) 307, 575, 951
Bsp1720I GCTNAGC 1 cut(s) 270
BspACI CCGC 4 cut(s) 248, 279, 288, 747
BspANI GGCC 2 cut(s) 247, 806
BspCNI CTCAG 2 cut(s) 211, 220
BspPI GGATC 1 cut(s) 583
BspTI CTTAAG 1 cut(s) 1067
BsrI ACTGG 4 cut(s) 42, 244, 423, 878
BssMI GATC 3 cut(s) 307, 575, 951
BssSI CACGAG 1 cut(s) 675
Bst2BI CACGAG 1 cut(s) 675
Bst2UI CCWGG 1 cut(s) 573
Bst4CI ACNGT 3 cut(s) 356, 383, 982
BstAFI CTTAAG 1 cut(s) 1067
BstAPI GCANNNNNTGC 1 cut(s) 447
BstBAI YACGTR 1 cut(s) 408
BstC8I GCNNGC 3 cut(s) 245, 896, 1041
BstDEI CTNAG 3 cut(s) 219, 228, 270
BstF5I GGATG 4 cut(s) 46, 350, 392, 688
BstKTI GATC 3 cut(s) 310, 578, 954
BstMAI GTCTC 3 cut(s) 111, 139, 343
BstMBI GATC 3 cut(s) 307, 575, 951
BstMWI GCNNNNNNNGC 7 cut(s) 179, 188, 210, 224, 285, 447, 1049
BstNI CCWGG 1 cut(s) 573
BstSCI CCNGG 1 cut(s) 571
BstV1I GCAGC 2 cut(s) 350, 469
BsuRI GGCC 2 cut(s) 247, 806
BtrI CACGTC 1 cut(s) 776
BtsCI GGATG 4 cut(s) 46, 350, 392, 688
BtsI GCAGTG 1 cut(s) 199
BtsIMutI CAGTG 5 cut(s) 199, 352, 379, 968, 987
Cac8I GCNNGC 3 cut(s) 245, 896, 1041
CaiI CAGNNNCTG 1 cut(s) 873
Cfr13I GGNCC 1 cut(s) 805
Csp6I GTAC 1 cut(s) 409
CviAII CATG 4 cut(s) 305, 329, 399, 949
CviQI GTAC 1 cut(s) 409
DdeI CTNAG 3 cut(s) 219, 228, 270
DpnI GATC 3 cut(s) 309, 577, 953
DpnII GATC 3 cut(s) 307, 575, 951
EaeI YGGCCR 1 cut(s) 245
Eco24I GRGCYC 1 cut(s) 939
EcoRII CCWGG 1 cut(s) 571
EcoT22I ATGCAT 1 cut(s) 392
EcoT38I GRGCYC 1 cut(s) 939
FaeI CATG 4 cut(s) 308, 332, 402, 952
FalI AAGNNNNNCTT 2 cut(s) 742, 774
FaqI GGGAC 3 cut(s) 508, 884, 989
FatI CATG 4 cut(s) 304, 328, 398, 948
FauI CCCGC 1 cut(s) 272
FbaI TGATCA 1 cut(s) 307
Fnu4HI GCNGC 3 cut(s) 248, 364, 483
FokI GGATG 4 cut(s) 53, 357, 399, 695
FriOI GRGCYC 1 cut(s) 939
Fsp4HI GCNGC 3 cut(s) 248, 364, 483
FspBI CTAG 3 cut(s) 630, 779, 1091
GluI GCNGC 3 cut(s) 248, 364, 483
HaeIII GGCC 2 cut(s) 247, 806
Hin1II CATG 4 cut(s) 308, 332, 402, 952
HindIII AAGCTT 1 cut(s) 591
HinfI GANTC 6 cut(s) 10, 375, 476, 518, 626, 837
Hpy166II GTNNAC 3 cut(s) 411, 635, 650
Hpy188I TCNGA 6 cut(s) 9, 196, 340, 517, 523, 826
Hpy188III TCNNGA 2 cut(s) 763, 854
Hpy8I GTNNAC 3 cut(s) 411, 635, 650
HpyAV CCTTC 3 cut(s) 485, 926, 1001
HpyCH4III ACNGT 3 cut(s) 356, 383, 982
HpyCH4IV ACGT 4 cut(s) 407, 497, 775, 986
HpyCH4V TGCA 6 cut(s) 213, 366, 390, 441, 599, 800
HpyF10VI GCNNNNNNNGC 7 cut(s) 179, 188, 210, 224, 285, 447, 1049
HpyF3I CTNAG 3 cut(s) 219, 228, 270
HpySE526I ACGT 4 cut(s) 407, 497, 775, 986
Hsp92II CATG 4 cut(s) 308, 332, 402, 952
Ksp22I TGATCA 1 cut(s) 307
Kzo9I GATC 3 cut(s) 307, 575, 951
Lsp1109I GCAGC 2 cut(s) 350, 469
LweI GCATC 4 cut(s) 377, 673, 706, 1026
MaeI CTAG 3 cut(s) 630, 779, 1091
MaeII ACGT 4 cut(s) 407, 497, 775, 986
MaeIII GTNAC 2 cut(s) 905, 982
MalI GATC 3 cut(s) 309, 577, 953
MboI GATC 3 cut(s) 307, 575, 951
MboII GAAGA 1 cut(s) 199
MhlI GDGCHC 2 cut(s) 201, 939
MluCI AATT 5 cut(s) 552, 819, 849, 878, 1077
MlyI GAGTC 3 cut(s) 4, 369, 485
MnlI CCTC 5 cut(s) 82, 422, 697, 735, 796
Mph1103I ATGCAT 1 cut(s) 392
MroXI GAANNNNTTC 2 cut(s) 324, 592
MseI TTAA 4 cut(s) 503, 743, 956, 1068
MslI CAYNNNNRTG 1 cut(s) 357
MspA1I CMGCKG 2 cut(s) 250, 482
MspCI CTTAAG 1 cut(s) 1067
MspR9I CCNGG 1 cut(s) 573
Mva1269I GAATGC 1 cut(s) 566
MvaI CCWGG 1 cut(s) 573
MwoI GCNNNNNNNGC 7 cut(s) 179, 188, 210, 224, 285, 447, 1049
NdeII GATC 3 cut(s) 307, 575, 951
NlaIII CATG 4 cut(s) 308, 332, 402, 952
NmuCI GTSAC 1 cut(s) 982
NsiI ATGCAT 1 cut(s) 392
PctI GAATGC 1 cut(s) 566
PdmI GAANNNNTTC 2 cut(s) 324, 592
PfeI GAWTC 3 cut(s) 518, 626, 837
PflMI CCANNNNNTGG 1 cut(s) 535
PfoI TCCNGGA 1 cut(s) 571
PkrI GCNGC 3 cut(s) 249, 365, 484
PleI GAGTC 3 cut(s) 4, 369, 484
PpsI GAGTC 3 cut(s) 4, 369, 484
Ppu21I YACGTR 1 cut(s) 408
Psp6I CCWGG 1 cut(s) 571
PspGI CCWGG 1 cut(s) 571
PspPI GGNCC 1 cut(s) 805
PstNI CAGNNNCTG 1 cut(s) 873
PvuII CAGCTG 1 cut(s) 482
RsaI GTAC 1 cut(s) 410
RsaNI GTAC 1 cut(s) 409
RseI CAYNNNNRTG 1 cut(s) 357
SaqAI TTAA 4 cut(s) 503, 743, 956, 1068
SatI GCNGC 3 cut(s) 248, 364, 483
Sau3AI GATC 3 cut(s) 307, 575, 951
Sau96I GGNCC 1 cut(s) 805
SchI GAGTC 3 cut(s) 4, 369, 485
ScrFI CCNGG 1 cut(s) 573
SduI GDGCHC 2 cut(s) 201, 939
SfaNI GCATC 4 cut(s) 377, 673, 706, 1026
SmiMI CAYNNNNRTG 1 cut(s) 357
SmlI CTYRAG 2 cut(s) 938, 1067
SmoI CTYRAG 2 cut(s) 938, 1067
Sse9I AATT 5 cut(s) 552, 819, 849, 878, 1077
SsiI CCGC 4 cut(s) 248, 279, 288, 747
SspMI CTAG 3 cut(s) 630, 779, 1091
StyD4I CCNGG 1 cut(s) 571
TaaI ACNGT 3 cut(s) 356, 383, 982
TaiI ACGT 4 cut(s) 410, 500, 778, 989
TaqI TCGA 2 cut(s) 707, 994
TasI AATT 5 cut(s) 552, 819, 849, 878, 1077
TauI GCSGC 1 cut(s) 250
TfiI GAWTC 3 cut(s) 518, 626, 837
Tru1I TTAA 4 cut(s) 503, 743, 956, 1068
Tru9I TTAA 4 cut(s) 503, 743, 956, 1068
TscAI CASTG 5 cut(s) 206, 359, 386, 968, 987
TseFI GTSAC 1 cut(s) 982
TseI GCWGC 2 cut(s) 363, 482
Tsp45I GTSAC 1 cut(s) 982
TspDTI ATGAA 4 cut(s) 223, 317, 504, 980
TspGWI ACGGA 1 cut(s) 428
TspRI CASTG 5 cut(s) 206, 359, 386, 968, 987
Van91I CCANNNNNTGG 1 cut(s) 535
Vha464I CTTAAG 1 cut(s) 1067
XapI RAATTY 3 cut(s) 819, 878, 1077
XmnI GAANNNNTTC 2 cut(s) 324, 592
XspI CTAG 3 cut(s) 630, 779, 1091
Zsp2I ATGCAT 1 cut(s) 392
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.