Rw5G022580

B3 DNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0001196
Physical Location & Seq
Reverse (-)
31371856 .. 31378854
6999 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G022580.1

Sequence Viewer

Length: 1239 bp
ATGGCTTTGACCCACACGCATTTATACTTCTGCCTCCGTCACTGTCTTTGCTTCTTGACTTCAATGGCTGCCAAAGCTTCGTGCTTTTTCTGCAAGGCTGACAGTGGTCAGTTCGTTAATGGCTGGGAGCTCAAGGATGGGTCTTTCGCCCAGCTCTGCAAGAAATGCGGTTTTGCTTATGTGAATGGTATATTTTGTGAAACTTTTCACATGGAAGCAGAAGGCTGGAGAGACTGTGCTGTTTGTGGGAAGCCACTCCATTGTGGGTGCATTATGTCACGCCACAAGTATGCACCACTGGATTTCGGAGGTGTAGGTTGCACAAAATGCTTACTAGAAGTTGATCAATCTATTGCTTCAGGTCGAATGACCAGAATGCTGAAAGAAATAGTTGCCAAGTACAAGATGGACCCAAAAGTTTCATCTGATCCTACTAAGAAAGTTTTTGAGTCCCTTTTTCCTGAATCTTTACAAGCTCCAGCACCTCAGATTGGAGAAGAATCCAATACGGATGCATCTAAAGGAGATACAGCTTGTGAAGCCAAAGTAGGCACTGAAACACCTCAAGTGGATGCCTCCACAGGAAACCATTTACACCCTGACAATCTGCCGAAGATGACAAGGGCTGAGCAACTCCAAAAAGTCTCCAGAAACTTAAACTCTGATCTTATACCTCTGTTTGAGAAGTCATCAATCGTAAGTGATACTAATCACAGTACAGGATTTCTTGTGATACCGAAGAAATATGCAGAGGAACAATCTCCAAGGGACATTGAAACAGGTCTAACACCTGTAGATACAAGTCTGAAAGCAGACCAGGAACAATCTCCAATGGATGTGGTAACAAATCAGAAAACAAACCAGGAACAATCTCCAAGGGACAATGAAACAGGTCCAACGCGTGTAGATACAACTCTGAAACCAGACCAGGAACAATCTCCGATGGTTATGGAAACAAGTCCAGTGCCTATAGAAACAAATCAGAAAACGAACCAGGAACAATCTCTAAGGGACTTTAAAGCAGGTCCAACACCTGTAGTTACAAGTCTGAAAACAGACCAGGAACAACCTTCAATGGATATGGAGACGAGTCCAATGCCTGTAGAAACAAGTCATAAATCAGACCAGGGACAATCTCCAAAGGATATGGAAACAGGTCCAACGCCTGTAGAAACAAGTCTGAAAACAGACCAGGAACAGTCTCCCAAGGGATATGGAAACAAGTCCAAAACAGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

412

Amino Acids

45.38

Weight (kDa)

5.34

Isoelectric Point (pI)

49.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf_VAL1_N PF25813 68 - 112 2.1e-21 VAL1-like, N-terminal zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1013
AccII CGCG 1 cut(s) 901
AciI CCGC 1 cut(s) 168
AclWI GGATC 1 cut(s) 422
AcuI CTGAAG 1 cut(s) 342
AfaI GTAC 2 cut(s) 401, 718
AfiI CCNNNNNNNGG 1 cut(s) 491
AflIII ACRYGT 1 cut(s) 899
AgsI TTSAA 3 cut(s) 63, 776, 1074
AjnI CCWGG 7 cut(s) 816, 861, 927, 993, 1059, 1125, 1191
AluBI AGCT 5 cut(s) 77, 130, 154, 476, 533
AluI AGCT 5 cut(s) 77, 130, 154, 476, 533
Alw21I GWGCWC 1 cut(s) 132
Alw26I GTCTC 4 cut(s) 225, 649, 1079, 1206
AlwI GGATC 1 cut(s) 422
ApeKI GCWGC 1 cut(s) 68
Asp700I GAANNNNTTC 1 cut(s) 204
AspS9I GGNCC 4 cut(s) 409, 893, 1025, 1157
AvaII GGWCC 4 cut(s) 409, 893, 1025, 1157
BanII GRGCYC 1 cut(s) 132
Bbv12I GWGCWC 1 cut(s) 132
BbvI GCAGC 1 cut(s) 55
BccI CCATC 3 cut(s) 131, 400, 937
BcgI CGANNNNNNTGC 2 cut(s) 1078, 1112
BciT130I CCWGG 7 cut(s) 818, 863, 929, 995, 1061, 1127, 1193
BclI TGATCA 1 cut(s) 343
BcoDI GTCTC 4 cut(s) 225, 649, 1079, 1206
BfaI CTAG 2 cut(s) 335, 1237
BfmI CTRYAG 5 cut(s) 792, 969, 1035, 1101, 1167
BfuAI ACCTGC 1 cut(s) 1013
BisI GCNGC 1 cut(s) 69
BlpI GCTNAGC 1 cut(s) 627
BlsI GCNGC 1 cut(s) 70
Bme1390I CCNGG 7 cut(s) 818, 863, 929, 995, 1061, 1127, 1193
Bme18I GGWCC 4 cut(s) 409, 893, 1025, 1157
BmgT120I GGNCC 4 cut(s) 409, 893, 1025, 1157
BmiI GGNNCC 1 cut(s) 411
BmrFI CCNGG 7 cut(s) 818, 863, 929, 995, 1061, 1127, 1193
BmsI GCATC 3 cut(s) 502, 524, 562
BoxI GACNNNNGTC 1 cut(s) 105
BpmI CTGGAG 3 cut(s) 247, 462, 631
Bpu1102I GCTNAGC 1 cut(s) 627
BpuEI CTTGAG 2 cut(s) 116, 549
BsaBI GATNNNNATC 1 cut(s) 708
BsaJI CCNNGG 4 cut(s) 764, 875, 1126, 1206
BsaXI ACNNNNNCTCC 2 cut(s) 220, 250
Bsc4I CCNNNNNNNGG 1 cut(s) 491
Bse1I ACTGG 2 cut(s) 303, 962
Bse8I GATNNNNATC 1 cut(s) 708
BseBI CCWGG 7 cut(s) 818, 863, 929, 995, 1061, 1127, 1193
BseDI CCNNGG 4 cut(s) 764, 875, 1126, 1206
BseGI GGATG 4 cut(s) 142, 517, 577, 841
BseJI GATNNNNATC 1 cut(s) 708
BseLI CCNNNNNNNGG 1 cut(s) 491
BseMII CTCAG 2 cut(s) 500, 618
BseNI ACTGG 2 cut(s) 303, 962
BseXI GCAGC 1 cut(s) 55
BseYI CCCAGC 2 cut(s) 123, 150
Bsh1236I CGCG 1 cut(s) 901
BsiHKAI GWGCWC 1 cut(s) 132
BslFI GGGAC 5 cut(s) 436, 782, 893, 1025, 1143
BslI CCNNNNNNNGG 1 cut(s) 491
BsmAI GTCTC 4 cut(s) 225, 649, 1079, 1206
BsmBI CGTCTC 1 cut(s) 1079
BsmFI GGGAC 5 cut(s) 436, 782, 893, 1025, 1143
BsmI GAATGC 1 cut(s) 381
Bsp1286I GDGCHC 1 cut(s) 132
Bsp143I GATC 3 cut(s) 343, 427, 664
Bsp1720I GCTNAGC 1 cut(s) 627
BspACI CCGC 1 cut(s) 168
BspCNI CTCAG 2 cut(s) 499, 619
BspFNI CGCG 1 cut(s) 901
BspLI GGNNCC 1 cut(s) 411
BspMI ACCTGC 1 cut(s) 1013
BspPI GGATC 1 cut(s) 422
BsrI ACTGG 2 cut(s) 303, 962
BssECI CCNNGG 4 cut(s) 764, 875, 1126, 1206
BssMI GATC 3 cut(s) 343, 427, 664
BssT1I CCWWGG 3 cut(s) 764, 875, 1206
Bst2UI CCWGG 7 cut(s) 818, 863, 929, 995, 1061, 1127, 1193
Bst4CI ACNGT 5 cut(s) 44, 104, 236, 716, 1200
BstAPI GCANNNNNTGC 2 cut(s) 165, 327
BstDEI CTNAG 4 cut(s) 435, 486, 627, 1007
BstF5I GGATG 4 cut(s) 142, 517, 577, 841
BstFNI CGCG 1 cut(s) 901
BstKTI GATC 3 cut(s) 346, 430, 667
BstMAI GTCTC 4 cut(s) 225, 649, 1079, 1206
BstMBI GATC 3 cut(s) 343, 427, 664
BstMWI GCNNNNNNNGC 5 cut(s) 74, 90, 165, 327, 539
BstNI CCWGG 7 cut(s) 818, 863, 929, 995, 1061, 1127, 1193
BstPAI GACNNNNGTC 1 cut(s) 105
BstSCI CCNGG 7 cut(s) 816, 861, 927, 993, 1059, 1125, 1191
BstSFI CTRYAG 5 cut(s) 792, 969, 1035, 1101, 1167
BstUI CGCG 1 cut(s) 901
BstV1I GCAGC 1 cut(s) 55
BtsCI GGATG 4 cut(s) 142, 517, 577, 841
BtsIMutI CAGTG 5 cut(s) 40, 109, 296, 552, 969
BveI ACCTGC 1 cut(s) 1013
Cfr13I GGNCC 4 cut(s) 409, 893, 1025, 1157
Csp6I GTAC 2 cut(s) 400, 717
CspCI CAANNNNNGTGG 2 cut(s) 819, 854
CviAII CATG 1 cut(s) 211
CviQI GTAC 2 cut(s) 400, 717
DdeI CTNAG 4 cut(s) 435, 486, 627, 1007
DpnI GATC 3 cut(s) 345, 429, 666
DpnII GATC 3 cut(s) 343, 427, 664
DraI TTTAAA 1 cut(s) 1018
Ecl136II GAGCTC 1 cut(s) 130
Eco130I CCWWGG 3 cut(s) 764, 875, 1206
Eco24I GRGCYC 1 cut(s) 132
Eco47I GGWCC 4 cut(s) 409, 893, 1025, 1157
Eco53kI GAGCTC 1 cut(s) 130
Eco57I CTGAAG 1 cut(s) 342
EcoICRI GAGCTC 1 cut(s) 130
EcoRII CCWGG 7 cut(s) 816, 861, 927, 993, 1059, 1125, 1191
EcoT14I CCWWGG 3 cut(s) 764, 875, 1206
EcoT22I ATGCAT 1 cut(s) 517
EcoT38I GRGCYC 1 cut(s) 132
ErhI CCWWGG 3 cut(s) 764, 875, 1206
Esp3I CGTCTC 1 cut(s) 1079
FaeI CATG 1 cut(s) 214
FaqI GGGAC 5 cut(s) 436, 782, 893, 1025, 1143
FatI CATG 1 cut(s) 210
FbaI TGATCA 1 cut(s) 343
Fnu4HI GCNGC 1 cut(s) 69
FokI GGATG 4 cut(s) 149, 524, 584, 848
FriOI GRGCYC 1 cut(s) 132
Fsp4HI GCNGC 1 cut(s) 69
FspBI CTAG 2 cut(s) 335, 1237
GluI GCNGC 1 cut(s) 69
GsaI CCCAGC 2 cut(s) 127, 154
GsuI CTGGAG 3 cut(s) 247, 462, 631
Hin1II CATG 1 cut(s) 214
HindIII AAGCTT 1 cut(s) 75
HinfI GANTC 4 cut(s) 449, 464, 500, 1090
Hpy188III TCNNGA 3 cut(s) 55, 461, 648
HpyAV CCTTC 2 cut(s) 215, 1080
HpyCH4III ACNGT 5 cut(s) 44, 104, 236, 716, 1200
HpyCH4V TGCA 7 cut(s) 93, 159, 270, 293, 321, 515, 749
HpyF10VI GCNNNNNNNGC 5 cut(s) 74, 90, 165, 327, 539
HpyF3I CTNAG 4 cut(s) 435, 486, 627, 1007
Hsp92II CATG 1 cut(s) 214
Ksp22I TGATCA 1 cut(s) 343
Kzo9I GATC 3 cut(s) 343, 427, 664
LmnI GCTCC 2 cut(s) 127, 481
Lsp1109I GCAGC 1 cut(s) 55
LweI GCATC 3 cut(s) 502, 524, 562
MaeI CTAG 2 cut(s) 335, 1237
MaeIII GTNAC 4 cut(s) 38, 276, 841, 1039
MalI GATC 3 cut(s) 345, 429, 666
MboI GATC 3 cut(s) 343, 427, 664
MboII GAAGA 3 cut(s) 509, 625, 751
MhlI GDGCHC 1 cut(s) 132
MluI ACGCGT 1 cut(s) 899
MlyI GAGTC 2 cut(s) 458, 1099
MmeI TCCRAC 3 cut(s) 920, 1052, 1184
MnlI CCTC 7 cut(s) 44, 302, 495, 573, 586, 684, 745
Mph1103I ATGCAT 1 cut(s) 517
MroXI GAANNNNTTC 1 cut(s) 204
MseI TTAA 3 cut(s) 117, 656, 1017
MslI CAYNNNNRTG 1 cut(s) 288
MspR9I CCNGG 7 cut(s) 818, 863, 929, 995, 1061, 1127, 1193
Mva1269I GAATGC 1 cut(s) 381
MvaI CCWGG 7 cut(s) 818, 863, 929, 995, 1061, 1127, 1193
MvnI CGCG 1 cut(s) 901
MwoI GCNNNNNNNGC 5 cut(s) 74, 90, 165, 327, 539
NdeII GATC 3 cut(s) 343, 427, 664
NlaIII CATG 1 cut(s) 214
NlaIV GGNNCC 1 cut(s) 411
NmuCI GTSAC 2 cut(s) 38, 276
NsiI ATGCAT 1 cut(s) 517
PctI GAATGC 1 cut(s) 381
PdmI GAANNNNTTC 1 cut(s) 204
PfeI GAWTC 2 cut(s) 464, 500
PkrI GCNGC 1 cut(s) 70
PleI GAGTC 2 cut(s) 457, 1098
PpsI GAGTC 2 cut(s) 457, 1098
PshAI GACNNNNGTC 1 cut(s) 105
Psp124BI GAGCTC 1 cut(s) 132
Psp6I CCWGG 7 cut(s) 816, 861, 927, 993, 1059, 1125, 1191
PspFI CCCAGC 2 cut(s) 123, 150
PspGI CCWGG 7 cut(s) 816, 861, 927, 993, 1059, 1125, 1191
PspN4I GGNNCC 1 cut(s) 411
PspPI GGNCC 4 cut(s) 409, 893, 1025, 1157
RsaI GTAC 2 cut(s) 401, 718
RsaNI GTAC 2 cut(s) 400, 717
RseI CAYNNNNRTG 1 cut(s) 288
SacI GAGCTC 1 cut(s) 132
SaqAI TTAA 3 cut(s) 117, 656, 1017
SatI GCNGC 1 cut(s) 69
Sau3AI GATC 3 cut(s) 343, 427, 664
Sau96I GGNCC 4 cut(s) 409, 893, 1025, 1157
SchI GAGTC 2 cut(s) 458, 1099
ScrFI CCNGG 7 cut(s) 818, 863, 929, 995, 1061, 1127, 1193
SduI GDGCHC 1 cut(s) 132
SfaNI GCATC 3 cut(s) 502, 524, 562
SfcI CTRYAG 5 cut(s) 792, 969, 1035, 1101, 1167
SinI GGWCC 4 cut(s) 409, 893, 1025, 1157
SmiMI CAYNNNNRTG 1 cut(s) 288
SmlI CTYRAG 2 cut(s) 131, 564
SmoI CTYRAG 2 cut(s) 131, 564
SsiI CCGC 1 cut(s) 168
SspMI CTAG 2 cut(s) 335, 1237
SstI GAGCTC 1 cut(s) 132
StyD4I CCNGG 7 cut(s) 816, 861, 927, 993, 1059, 1125, 1191
StyI CCWWGG 3 cut(s) 764, 875, 1206
TaaI ACNGT 5 cut(s) 44, 104, 236, 716, 1200
TaqI TCGA 1 cut(s) 364
TatI WGTACW 2 cut(s) 399, 716
TfiI GAWTC 2 cut(s) 464, 500
Tru1I TTAA 3 cut(s) 117, 656, 1017
Tru9I TTAA 3 cut(s) 117, 656, 1017
TscAI CASTG 5 cut(s) 47, 109, 303, 559, 969
TseFI GTSAC 2 cut(s) 38, 276
TseI GCWGC 1 cut(s) 68
Tsp45I GTSAC 2 cut(s) 38, 276
TspDTI ATGAA 2 cut(s) 411, 900
TspGWI ACGGA 2 cut(s) 26, 524
TspRI CASTG 5 cut(s) 47, 109, 303, 559, 969
VpaK11BI GGWCC 4 cut(s) 409, 893, 1025, 1157
XcmI CCANNNNNNNNNTGG 1 cut(s) 403
XmnI GAANNNNTTC 1 cut(s) 204
XspI CTAG 2 cut(s) 335, 1237
Zsp2I ATGCAT 1 cut(s) 517
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.