Rh5BG245700

B3 DNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0001196
Physical Location & Seq
Reverse (-)
29833977 .. 29837951
3975 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG245700.1

Sequence Viewer

Length: 1053 bp
ATGGAAGCAGAAAGCTGGAGAGACTGTGCTGTTTGTGGGAAGCCACTCCATTGTGGGTGCATTATGTCACGCCACAAGGATGCACCACTGGATTTCGGAGGTGTAGGTTGCACAAAATGCTTACTAGACGTTGATCAATCTATTGCTTCAGGTCGAATGACCAGAATGGTGAAAGACATAGAAAATATTGTAACATGGCTGAATGAGGGACTGACCACTGACCAAAGCCAGGTCTTTGCTATTGCGGTTGCCAAGTACAAGACGGACCCAACAGTGTCATCTGATCCTACTAAGAAAGTTGTCGAGTCTCTTTTTCCTGAATCTTTACAAGCTCCAGCACCTCAGATTGGAGAAGAATCCAATACGGATGTATCTAAAGGAGCTACAGCTTGTGAATTCAAAGTAGACAGTGAAAAACCTCAAGTGGATGCCTCCACAGGAAACCATTTACACCCTGACAATCTGCTGAAGATGACAAGGGCTGAGCAACTCCAAAAAGTCTCCGGAAATACAGGATTTCTTGTGATACCGAAGAAATATGCAGAGGAACAATCTCCGAGGGACATTGAAACAGGTCCAACACCTGTATATACAAGTCTGAGAGCAGACCAGGAACAATCTCCAATGGATGTGGTAACAAATCCAATGCCTATAGAAACAAATCAGAAAACAAACCAGGAACAATCTCCAAGGGACATTGAAACAGGTCCAACGCGTGTAGATACAACCCTGAAACCAGACCAGGAACAATCTCTGATGGTAATGGAAACAAGTCCAATGCCTATAGAAACAAATCAGAAAACGAACCAGGAACAATCTCTAAGGGACTTTGAAACAGGTCCAACGTCTGTAGATACAAGTCTGAAAACAGACCAGGAACAACCTTCAAAGGATATGGAGACAAGTCCAATGCCTGTAGAAACAAGTCTTAAATCAGACCAGGGACAATCTCCAAAGGATATGGAAACAGGTCCAACGCCTGTAGAAACAAGTCTGAAAACAGACCAGGAACAGTCTCCCAAGGGTATGGAAACAAGTCCGAAAACAGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

350

Amino Acids

38.41

Weight (kDa)

4.62

Isoelectric Point (pI)

56.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf_VAL1_N PF25813 3 - 41 6.9e-16 VAL1-like, N-terminal zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 405
AccII CGCG 1 cut(s) 715
AccIII TCCGGA 1 cut(s) 503
AciI CCGC 1 cut(s) 245
AclWI GGATC 1 cut(s) 278
AcsI RAATTY 1 cut(s) 395
AcuI CTGAAG 2 cut(s) 132, 488
AfaI GTAC 1 cut(s) 257
AfiI CCNNNNNNNGG 2 cut(s) 229, 347
AflIII ACRYGT 1 cut(s) 713
AgsI TTSAA 5 cut(s) 400, 569, 701, 833, 888
AjnI CCWGG 8 cut(s) 228, 609, 675, 741, 807, 873, 939, 1005
AluBI AGCT 4 cut(s) 15, 332, 383, 389
AluI AGCT 4 cut(s) 15, 332, 383, 389
Alw26I GTCTC 5 cut(s) 15, 312, 505, 893, 1020
AlwI GGATC 1 cut(s) 278
Aor13HI TCCGGA 1 cut(s) 503
ApoI RAATTY 1 cut(s) 395
AspS9I GGNCC 5 cut(s) 265, 575, 707, 839, 971
AsuHPI GGTGA 1 cut(s) 181
AvaII GGWCC 5 cut(s) 265, 575, 707, 839, 971
BaeI ACNNNNGTAYC 2 cut(s) 354, 387
BccI CCATC 1 cut(s) 751
BciT130I CCWGG 8 cut(s) 230, 611, 677, 743, 809, 875, 941, 1007
BclI TGATCA 1 cut(s) 133
BcoDI GTCTC 5 cut(s) 15, 312, 505, 893, 1020
BfaI CTAG 2 cut(s) 125, 1051
BfmI CTRYAG 6 cut(s) 384, 651, 783, 849, 915, 981
BlpI GCTNAGC 1 cut(s) 483
Bme1390I CCNGG 8 cut(s) 230, 611, 677, 743, 809, 875, 941, 1007
Bme18I GGWCC 5 cut(s) 265, 575, 707, 839, 971
BmgT120I GGNCC 5 cut(s) 265, 575, 707, 839, 971
BmiI GGNNCC 1 cut(s) 267
BmrFI CCNGG 8 cut(s) 230, 611, 677, 743, 809, 875, 941, 1007
BmsI GCATC 2 cut(s) 70, 418
BpmI CTGGAG 2 cut(s) 37, 318
Bpu1102I GCTNAGC 1 cut(s) 483
BpuEI CTTGAG 1 cut(s) 405
BsaJI CCNNGG 4 cut(s) 557, 689, 940, 1020
BsaWI WCCGGW 1 cut(s) 503
BsaXI ACNNNNNCTCC 2 cut(s) 10, 40
Bsc4I CCNNNNNNNGG 2 cut(s) 229, 347
Bse1I ACTGG 1 cut(s) 93
BseAI TCCGGA 1 cut(s) 503
BseBI CCWGG 8 cut(s) 230, 611, 677, 743, 809, 875, 941, 1007
BseDI CCNNGG 4 cut(s) 557, 689, 940, 1020
BseGI GGATG 4 cut(s) 85, 373, 433, 634
BseLI CCNNNNNNNGG 2 cut(s) 229, 347
BseMII CTCAG 3 cut(s) 356, 474, 590
BseNI ACTGG 1 cut(s) 93
Bsh1236I CGCG 1 cut(s) 715
BsiSI CCGG 1 cut(s) 504
BslFI GGGAC 5 cut(s) 222, 575, 707, 839, 957
BslI CCNNNNNNNGG 2 cut(s) 229, 347
BsmAI GTCTC 5 cut(s) 15, 312, 505, 893, 1020
BsmFI GGGAC 5 cut(s) 222, 575, 707, 839, 957
Bsp13I TCCGGA 1 cut(s) 503
Bsp143I GATC 2 cut(s) 133, 283
Bsp1720I GCTNAGC 1 cut(s) 483
BspACI CCGC 1 cut(s) 245
BspCNI CTCAG 3 cut(s) 355, 475, 591
BspEI TCCGGA 1 cut(s) 503
BspFNI CGCG 1 cut(s) 715
BspLI GGNNCC 1 cut(s) 267
BspPI GGATC 1 cut(s) 278
BsrI ACTGG 1 cut(s) 93
BssECI CCNNGG 4 cut(s) 557, 689, 940, 1020
BssMI GATC 2 cut(s) 133, 283
BssT1I CCWWGG 2 cut(s) 689, 1020
Bst2UI CCWGG 8 cut(s) 230, 611, 677, 743, 809, 875, 941, 1007
Bst4CI ACNGT 4 cut(s) 26, 274, 410, 1014
BstAPI GCANNNNNTGC 1 cut(s) 117
BstDEI CTNAG 5 cut(s) 291, 342, 483, 599, 821
BstF5I GGATG 4 cut(s) 85, 373, 433, 634
BstFNI CGCG 1 cut(s) 715
BstKTI GATC 2 cut(s) 136, 286
BstMAI GTCTC 5 cut(s) 15, 312, 505, 893, 1020
BstMBI GATC 2 cut(s) 133, 283
BstMWI GCNNNNNNNGC 1 cut(s) 117
BstNI CCWGG 8 cut(s) 230, 611, 677, 743, 809, 875, 941, 1007
BstSCI CCNGG 8 cut(s) 228, 609, 675, 741, 807, 873, 939, 1005
BstSFI CTRYAG 6 cut(s) 384, 651, 783, 849, 915, 981
BstUI CGCG 1 cut(s) 715
BstXI CCANNNNNNTGG 1 cut(s) 1027
BtsCI GGATG 4 cut(s) 85, 373, 433, 634
BtsIMutI CAGTG 4 cut(s) 86, 216, 279, 415
Cfr13I GGNCC 5 cut(s) 265, 575, 707, 839, 971
Csp6I GTAC 1 cut(s) 256
CspCI CAANNNNNGTGG 2 cut(s) 612, 647
CviAII CATG 1 cut(s) 195
CviJI RGCY 8 cut(s) 15, 43, 199, 228, 332, 383, 389, 482
CviKI_1 RGCY 8 cut(s) 15, 43, 199, 228, 332, 383, 389, 482
CviQI GTAC 1 cut(s) 256
DdeI CTNAG 5 cut(s) 291, 342, 483, 599, 821
DpnI GATC 2 cut(s) 135, 285
DpnII GATC 2 cut(s) 133, 283
Eco130I CCWWGG 2 cut(s) 689, 1020
Eco47I GGWCC 5 cut(s) 265, 575, 707, 839, 971
Eco57I CTGAAG 2 cut(s) 132, 488
EcoRI GAATTC 1 cut(s) 395
EcoRII CCWGG 8 cut(s) 228, 609, 675, 741, 807, 873, 939, 1005
EcoT14I CCWWGG 2 cut(s) 689, 1020
ErhI CCWWGG 2 cut(s) 689, 1020
FaeI CATG 1 cut(s) 198
FaqI GGGAC 5 cut(s) 222, 575, 707, 839, 957
FatI CATG 1 cut(s) 194
FbaI TGATCA 1 cut(s) 133
FblI GTMKAC 1 cut(s) 405
FokI GGATG 4 cut(s) 92, 380, 440, 641
FspBI CTAG 2 cut(s) 125, 1051
GsuI CTGGAG 2 cut(s) 37, 318
HapII CCGG 1 cut(s) 504
Hin1II CATG 1 cut(s) 198
HinfI GANTC 3 cut(s) 305, 320, 356
HpaII CCGG 1 cut(s) 504
HphI GGTGA 1 cut(s) 181
Hpy166II GTNNAC 1 cut(s) 406
Hpy188III TCNNGA 2 cut(s) 317, 504
Hpy8I GTNNAC 1 cut(s) 406
HpyAV CCTTC 1 cut(s) 894
HpyCH4III ACNGT 4 cut(s) 26, 274, 410, 1014
HpyCH4IV ACGT 2 cut(s) 129, 845
HpyCH4V TGCA 4 cut(s) 60, 83, 111, 542
HpyF10VI GCNNNNNNNGC 1 cut(s) 117
HpyF3I CTNAG 5 cut(s) 291, 342, 483, 599, 821
HpySE526I ACGT 2 cut(s) 129, 845
Hsp92II CATG 1 cut(s) 198
Kpn2I TCCGGA 1 cut(s) 503
Ksp22I TGATCA 1 cut(s) 133
Kzo9I GATC 2 cut(s) 133, 283
LmnI GCTCC 2 cut(s) 337, 380
LweI GCATC 2 cut(s) 70, 418
MaeI CTAG 2 cut(s) 125, 1051
MaeII ACGT 2 cut(s) 129, 845
MaeIII GTNAC 3 cut(s) 66, 190, 634
MalI GATC 2 cut(s) 135, 285
MboI GATC 2 cut(s) 133, 283
MboII GAAGA 3 cut(s) 365, 481, 544
MluCI AATT 1 cut(s) 395
MluI ACGCGT 1 cut(s) 713
MlyI GAGTC 1 cut(s) 314
MmeI TCCRAC 4 cut(s) 602, 734, 866, 998
MnlI CCTC 7 cut(s) 92, 199, 351, 429, 442, 538, 552
MroI TCCGGA 1 cut(s) 503
MseI TTAA 1 cut(s) 930
MslI CAYNNNNRTG 1 cut(s) 78
MspI CCGG 1 cut(s) 504
MspR9I CCNGG 8 cut(s) 230, 611, 677, 743, 809, 875, 941, 1007
MvaI CCWGG 8 cut(s) 230, 611, 677, 743, 809, 875, 941, 1007
MvnI CGCG 1 cut(s) 715
MwoI GCNNNNNNNGC 1 cut(s) 117
NdeII GATC 2 cut(s) 133, 283
NlaIII CATG 1 cut(s) 198
NlaIV GGNNCC 1 cut(s) 267
NmuCI GTSAC 1 cut(s) 66
PfeI GAWTC 2 cut(s) 320, 356
PleI GAGTC 1 cut(s) 313
PpsI GAGTC 1 cut(s) 313
Psp6I CCWGG 8 cut(s) 228, 609, 675, 741, 807, 873, 939, 1005
PspGI CCWGG 8 cut(s) 228, 609, 675, 741, 807, 873, 939, 1005
PspN4I GGNNCC 1 cut(s) 267
PspPI GGNCC 5 cut(s) 265, 575, 707, 839, 971
RsaI GTAC 1 cut(s) 257
RsaNI GTAC 1 cut(s) 256
RseI CAYNNNNRTG 1 cut(s) 78
SaqAI TTAA 1 cut(s) 930
Sau3AI GATC 2 cut(s) 133, 283
Sau96I GGNCC 5 cut(s) 265, 575, 707, 839, 971
SchI GAGTC 1 cut(s) 314
ScrFI CCNGG 8 cut(s) 230, 611, 677, 743, 809, 875, 941, 1007
SfaNI GCATC 2 cut(s) 70, 418
SfcI CTRYAG 6 cut(s) 384, 651, 783, 849, 915, 981
SinI GGWCC 5 cut(s) 265, 575, 707, 839, 971
SmiMI CAYNNNNRTG 1 cut(s) 78
SmlI CTYRAG 1 cut(s) 420
SmoI CTYRAG 1 cut(s) 420
Sse9I AATT 1 cut(s) 395
SsiI CCGC 1 cut(s) 245
SspI AATATT 1 cut(s) 187
SspMI CTAG 2 cut(s) 125, 1051
StyD4I CCNGG 8 cut(s) 228, 609, 675, 741, 807, 873, 939, 1005
StyI CCWWGG 2 cut(s) 689, 1020
TaaI ACNGT 4 cut(s) 26, 274, 410, 1014
TaiI ACGT 2 cut(s) 132, 848
TaqI TCGA 2 cut(s) 154, 303
TasI AATT 1 cut(s) 395
TatI WGTACW 1 cut(s) 255
TfiI GAWTC 2 cut(s) 320, 356
Tru1I TTAA 1 cut(s) 930
Tru9I TTAA 1 cut(s) 930
TscAI CASTG 4 cut(s) 93, 223, 279, 415
TseFI GTSAC 1 cut(s) 66
Tsp45I GTSAC 1 cut(s) 66
TspGWI ACGGA 2 cut(s) 278, 380
TspRI CASTG 4 cut(s) 93, 223, 279, 415
VpaK11BI GGWCC 5 cut(s) 265, 575, 707, 839, 971
XapI RAATTY 1 cut(s) 395
XmiI GTMKAC 1 cut(s) 405
XspI CTAG 2 cut(s) 125, 1051
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.