Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb5

Orthogroup ID

OG0001526

Physical Location & Seq

Genomic Coordinates Forward (+)

23991649 .. 23994532

Gene Structure 2884 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_5g33190.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1512 bp

ATGGCCTCGCTCTTGAGCTCAACCCAACCTCTGCCACCGGAGTTTAACCGAGGACGGGCTCCGGCCACCAAAACGGCAGCTCCACGGGCGCAGACGACGCCCCATCCTCAACCCACTAGTGCCGATAGCAACACTAAACTCGCAGTAGTAACAGAGAGACAAGCAGCAGAGCTTAAGAGGCTAAAACATAAGGAGAGCAAGGACAGGAAGGAGGAGACCAACAAGAAGATAGCTTCTCGGAAAGCAATCTCGGTCGTTTTAATGAGAGAAGCTACCAAATCCCACATCGAGAAGAAGAAAGGCTCCAAGAGACTGCTACCCAGGACTGTTCTCGAAGCCCTCCATGAGAGAATTACTGCTCTGCGCTGGGAGTCTGCTCTCAAGGTTTTTCAACTACTACAAGAGCAGTTGTGGTACAGGCCCAATCCTGGTGTTTACATTAAGCTAATTGTCATGCTAGGAAAATGTAAACAACCAGAAAAAGCCGAGGAGCTTTTCCAAGCTATGATTGACGAAGGCTGTGGTGTCAGCCATGAATCTTATACTGCTCTTATATCTGCCTATGGTAGGAGCAGTATCTTCGACAAAGCATTTTCCCTCCTTGAGCAGATGAAGGATACTCCTGACTGTCAACCTGATGTCCATACTTATTCAATCCTCATAAAGTCTTGCTTGCATGTTTTCGCATACGACAGAGTACAGGCTCTGCTTTCAGATATGGAAACTCAGCACATTAGACCCAACACCATCACATACAATACCCTAATTGATGCTTACGGGAAATCTAAAAGATTTGCAGAGATGGAATCGACACTTGTGGCAATGCTTAGCCAACGGGATTGTGAGCCTGATGTCTGGACCATGAATTCTGTAGTGAGAGCCTTTGGCAGCAGTGGGCAAATAGAAACAATGGAAAAATGTTTTGAGAGATTCCACAGTGCCGGAATCCAACCAAACATCATGACTTTCAACATTCTCCTCGATTCATATGGAAAATCTGGGAATTATAAGAAAATGAGTGCTGTAATGGAATACATGCAGAAATACCATTATTCATGGACAATCGTAACCTATAATGTGGTGATAGATGCATTTGGGAGGGCTGGGGATTTAACACAAATGGAGTATCTGTTTAGGCTAATGCAGTCAGAGCGGATCAAGCCAAGCTGTGTCACACTTTGCTCACTCGTAAGAGCCTATGGGAAAGCAGGTAAACCTGAAAAAATTGGTGGTGTTTTATGTTTTGTGGAGAATTCAGACGTGAAGCTGGATGCCGTGTTTTTCAATTGTCTGGTTGATGCTTATGGGAGGCTGGGATGCTTCACAGAAATGAAAGGGGTGCTTGAGATGATGGAGCAAAAAGGATGTTCTCCAGATAAGATCACATATAGAACCATGATTAAAGCTTATTCATTCAACAAGATGACTGGCCATGTGAAGGAACTCCATGAACTCTTGCAATCGGCAGGAAGTCAGATGAGTTGGTCACAGAGACAAAAGCCGGACTTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2 PPR_3

Protein Analysis

504

Amino Acids

57.08

Weight (kDa)

9.07

Isoelectric Point (pI)

38.7

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	180 - 225	2.9e-11	PPR repeat family
PPR_3	PF13812	200 - 261	1.3e-09	Pentatricopeptide repeat domain
PPR_2	PF13041	247 - 295	1.6e-08	PPR repeat family
PPR_3	PF13812	276 - 332	1.1e-07	Pentatricopeptide repeat domain
PPR_2	PF13041	283 - 331	8.3e-08	PPR repeat family
PPR_2	PF13041	355 - 401	6.1e-08	PPR repeat family
PPR_3	PF13812	416 - 470	7.5e-06	Pentatricopeptide repeat domain
PPR_2	PF13041	424 - 470	4.8e-10	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001526)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G48730
fragaria_vesca	FvH4_5g33190
malus_domestica	MD10G1163400.v1.1
prunus_persica	Prupe.8G201700_v2.0.a1 Prupe.8G201700_v2.0.a1 Prupe.8G201700_v2.0.a1
pyrus_communis	pycom10g14080
rosa_chinensis	RchiOBHm_Chr1g0373361 RchiOBHm_Chr7g0231531
rosa_laevigata	RLG00000001413 RLG00000008268
rosa_multiflora	Rmu_sc0001565.1_g000030 Rmu_sc0003505.1_g000027 Rmu_sc0005399.1_g000019 Rmu_sc0011314.1_g000012 Rmu_sc0012399.1_g000004 Rmu_sc0013558.1_g000012 Rmu_sc0028716.1_g000001
rosa_roxburghii	Rroxscaffold_1G00016380 Rroxscaffold_1G00029710 Rroxscaffold_3G00229090
rosa_rugosa	Rorug07G0267100 Rorug07G0267100
rosa_samantha	Rh1AG365000 Rh1CG099500 Rh2AG510800 Rh2BG430100 Rh2BG476000 Rh2CG061700 Rh2CG496000 Rh2DG388600 Rh2DG440000 Rh2DG484700 Rh4BG006500 Rh4DG135300 Rh5AG195600 Rh5AG195700 Rh5CG115300 Rh6AG146300 Rh7AG421500 Rh7BG396200 Rh7CG441800 Rh7DG259000 Rh7DG414200
rosa_wichuraiana	Rw0G012110 Rw6G012720 Rw6G032340 Rw6G045030 Rw7G034780 Rw7G034910 Rw7G034940

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1008
Acc36I	ACCTGC	1 cut(s)	1199
AccBSI	CCGCTC	1 cut(s)	1153
AciI	CCGC	1 cut(s)	1153
AclWI	GGATC	1 cut(s)	1163
AcoI	YGGCCR	2 cut(s)	63, 1429
AcsI	RAATTY	2 cut(s)	865, 1252
AcyI	GRCGYC	1 cut(s)	98
AfaI	GTAC	2 cut(s)	416, 699
AfiI	CCNNNNNNNGG	4 cut(s)	55, 428, 567, 1438
AflII	CTTAAG	1 cut(s)	173
AgsI	TTSAA	5 cut(s)	392, 654, 970, 1285, 1417
AhlI	ACTAGT	1 cut(s)	116
AjiI	CACGTC	1 cut(s)	1261
AjnI	CCWGG	2 cut(s)	320, 427
Alw21I	GWGCWC	1 cut(s)	20
Alw26I	GTCTC	4 cut(s)	151, 209, 304, 1486
AlwI	GGATC	1 cut(s)	1163
AlwNI	CAGNNNCTG	1 cut(s)	706
AoxI	GGCC	4 cut(s)	3, 63, 419, 1429
ApeKI	GCWGC	3 cut(s)	77, 164, 888
ApoI	RAATTY	2 cut(s)	865, 1252
AspLEI	GCGC	2 cut(s)	91, 366
AspS9I	GGNCC	2 cut(s)	420, 858
AsuHPI	GGTGA	1 cut(s)	1093
AvaII	GGWCC	1 cut(s)	858
BalI	TGGCCA	1 cut(s)	1431
BanII	GRGCYC	2 cut(s)	20, 61
Bbv12I	GWGCWC	1 cut(s)	20
BbvI	GCAGC	3 cut(s)	89, 176, 900
BccI	CCATC	4 cut(s)	111, 755, 796, 1345
BceAI	ACGGC	2 cut(s)	90, 1259
BcgI	CGANNNNNNTGC	2 cut(s)	562, 596
BciT130I	CCWGG	2 cut(s)	322, 429
BciVI	GTATCC	1 cut(s)	610
BcoDI	GTCTC	4 cut(s)	151, 209, 304, 1486
BcuI	ACTAGT	1 cut(s)	116
BfaI	CTAG	2 cut(s)	117, 458
BfmI	CTRYAG	1 cut(s)	870
BfrI	CTTAAG	1 cut(s)	173
BfuAI	ACCTGC	1 cut(s)	1199
BfuI	GTATCC	1 cut(s)	610
BisI	GCNGC	3 cut(s)	78, 165, 889
BlpI	GCTNAGC	1 cut(s)	827
BlsI	GCNGC	3 cut(s)	79, 166, 890
Bme1390I	CCNGG	2 cut(s)	322, 429
Bme18I	GGWCC	1 cut(s)	858
BmgBI	CACGTC	1 cut(s)	1261
BmgT120I	GGNCC	2 cut(s)	420, 858
BmiI	GGNNCC	2 cut(s)	60, 304
BmrFI	CCNGG	2 cut(s)	322, 429
BmsI	GCATC	5 cut(s)	760, 1078, 1261, 1288, 1307
BpmI	CTGGAG	1 cut(s)	1356
Bpu1102I	GCTNAGC	1 cut(s)	827
BpuEI	CTTGAG	4 cut(s)	34, 365, 623, 1364
BsaHI	GRCGYC	1 cut(s)	98
BsaI	GGTCTC	1 cut(s)	209
BsaJI	CCNNGG	4 cut(s)	49, 83, 320, 486
BsaWI	WCCGGW	1 cut(s)	37
BsaXI	ACNNNNNCTCC	4 cut(s)	64, 94, 1115, 1145
Bsc4I	CCNNNNNNNGG	4 cut(s)	55, 428, 567, 1438
Bse1I	ACTGG	1 cut(s)	1432
Bse3DI	GCAATG	1 cut(s)	828
BseBI	CCWGG	2 cut(s)	322, 429
BseDI	CCNNGG	4 cut(s)	49, 83, 320, 486
BseGI	GGATG	4 cut(s)	103, 1276, 1322, 1370
BseLI	CCNNNNNNNGG	4 cut(s)	55, 428, 567, 1438
BseMI	GCAATG	1 cut(s)	828
BseMII	CTCAG	1 cut(s)	740
BseNI	ACTGG	1 cut(s)	1432
BseRI	GAGGAG	3 cut(s)	227, 503, 968
BseXI	GCAGC	3 cut(s)	89, 176, 900
BseYI	CCCAGC	3 cut(s)	366, 1103, 1312
Bsh1285I	CGRYCG	1 cut(s)	255
BshFI	GGCC	4 cut(s)	5, 65, 421, 1431
BsiEI	CGRYCG	1 cut(s)	255
BsiHKAI	GWGCWC	1 cut(s)	20
BsiSI	CCGG	4 cut(s)	38, 62, 942, 1502
BslI	CCNNNNNNNGG	4 cut(s)	55, 428, 567, 1438
BsmAI	GTCTC	4 cut(s)	151, 209, 304, 1486
BsnI	GGCC	4 cut(s)	5, 65, 421, 1431
Bso31I	GGTCTC	1 cut(s)	209
Bsp1286I	GDGCHC	2 cut(s)	20, 61
Bsp143I	GATC	2 cut(s)	1155, 1380
Bsp1720I	GCTNAGC	1 cut(s)	827
BspACI	CCGC	1 cut(s)	1153
BspANI	GGCC	4 cut(s)	5, 65, 421, 1431
BspCNI	CTCAG	1 cut(s)	739
BspHI	TCATGA	1 cut(s)	960
BspLI	GGNNCC	2 cut(s)	60, 304
BspMI	ACCTGC	1 cut(s)	1199
BspPI	GGATC	1 cut(s)	1163
BspTI	CTTAAG	1 cut(s)	173
BspTNI	GGTCTC	1 cut(s)	209
BsrBI	CCGCTC	1 cut(s)	1153
BsrDI	GCAATG	1 cut(s)	828
BsrI	ACTGG	1 cut(s)	1432
BssECI	CCNNGG	4 cut(s)	49, 83, 320, 486
BssMI	GATC	2 cut(s)	1155, 1380
BssNI	GRCGYC	1 cut(s)	98
Bst2UI	CCWGG	2 cut(s)	322, 429
Bst4CI	ACNGT	3 cut(s)	328, 629, 938
BstACI	GRCGYC	1 cut(s)	98
BstAFI	CTTAAG	1 cut(s)	173
BstC8I	GCNNGC	1 cut(s)	674
BstDEI	CTNAG	2 cut(s)	726, 827
BstDSI	CCRYGG	1 cut(s)	83
BstENI	CCTNNNNNAGG	1 cut(s)	565
BstF5I	GGATG	4 cut(s)	103, 1276, 1322, 1370
BstHHI	GCGC	2 cut(s)	91, 366
BstKTI	GATC	2 cut(s)	1158, 1383
BstMAI	GTCTC	4 cut(s)	151, 209, 304, 1486
BstMBI	GATC	2 cut(s)	1155, 1380
BstMCI	CGRYCG	1 cut(s)	255
BstMWI	GCNNNNNNNGC	5 cut(s)	86, 97, 178, 1150, 1159
BstNI	CCWGG	2 cut(s)	322, 429
BstNSI	RCATGY	2 cut(s)	680, 1039
BstSCI	CCNGG	2 cut(s)	320, 427
BstSFI	CTRYAG	1 cut(s)	870
BstV1I	GCAGC	3 cut(s)	89, 176, 900
BsuI	GTATCC	1 cut(s)	610
BsuRI	GGCC	4 cut(s)	5, 65, 421, 1431
BtgI	CCRYGG	1 cut(s)	83
BtrI	CACGTC	1 cut(s)	1261
BtsCI	GGATG	4 cut(s)	103, 1276, 1322, 1370
BtsI	GCAGTG	1 cut(s)	898
BtsIMutI	CAGTG	2 cut(s)	898, 943
BveI	ACCTGC	1 cut(s)	1199
Cac8I	GCNNGC	1 cut(s)	674
CaiI	CAGNNNCTG	1 cut(s)	706
CciI	TCATGA	1 cut(s)	960
CfoI	GCGC	2 cut(s)	91, 366
Cfr13I	GGNCC	2 cut(s)	420, 858
CseI	GACGC	1 cut(s)	106
Csp6I	GTAC	2 cut(s)	415, 698
CviQI	GTAC	2 cut(s)	415, 698
DdeI	CTNAG	2 cut(s)	726, 827
DpnI	GATC	2 cut(s)	1157, 1382
DpnII	GATC	2 cut(s)	1155, 1380
EaeI	YGGCCR	2 cut(s)	63, 1429
Ecl136II	GAGCTC	1 cut(s)	18
Eco24I	GRGCYC	2 cut(s)	20, 61
Eco31I	GGTCTC	1 cut(s)	209
Eco47I	GGWCC	1 cut(s)	858
Eco53kI	GAGCTC	1 cut(s)	18
EcoICRI	GAGCTC	1 cut(s)	18
EcoNI	CCTNNNNNAGG	1 cut(s)	565
EcoRI	GAATTC	2 cut(s)	865, 1252
EcoRII	CCWGG	2 cut(s)	320, 427
EcoT22I	ATGCAT	1 cut(s)	1093
EcoT38I	GRGCYC	2 cut(s)	20, 61
FalI	AAGNNNNNCTT	5 cut(s)	656, 688, 1326, 1358, 1490
FauNDI	CATATG	1 cut(s)	988
Fnu4HI	GCNGC	3 cut(s)	78, 165, 889
FokI	GGATG	4 cut(s)	90, 1283, 1329, 1377
FriOI	GRGCYC	2 cut(s)	20, 61
Fsp4HI	GCNGC	3 cut(s)	78, 165, 889
FspBI	CTAG	2 cut(s)	117, 458
GlaI	GCGC	2 cut(s)	90, 365
GluI	GCNGC	3 cut(s)	78, 165, 889
GsaI	CCCAGC	3 cut(s)	370, 1107, 1316
GsuI	CTGGAG	1 cut(s)	1356
HaeIII	GGCC	4 cut(s)	5, 65, 421, 1431
HapII	CCGG	4 cut(s)	38, 62, 942, 1502
HgaI	GACGC	1 cut(s)	106
HhaI	GCGC	2 cut(s)	91, 366
Hin1I	GRCGYC	1 cut(s)	98
Hin6I	GCGC	2 cut(s)	89, 364
HinP1I	GCGC	2 cut(s)	89, 364
HincII	GTYRAC	1 cut(s)	632
HindII	GTYRAC	1 cut(s)	632
HindIII	AAGCTT	1 cut(s)	1404
HinfI	GANTC	6 cut(s)	371, 536, 806, 930, 945, 983
HpaII	CCGG	4 cut(s)	38, 62, 942, 1502
HphI	GGTGA	1 cut(s)	1093
Hpy166II	GTNNAC	4 cut(s)	436, 470, 632, 1214
Hpy188I	TCNGA	5 cut(s)	240, 715, 1150, 1258, 1476
Hpy188III	TCNNGA	7 cut(s)	13, 289, 332, 623, 856, 961, 1373
Hpy8I	GTNNAC	4 cut(s)	436, 470, 632, 1214
Hpy99I	CGWCG	1 cut(s)	100
HpyAV	CCTTC	4 cut(s)	202, 509, 607, 1432
HpyCH4III	ACNGT	3 cut(s)	328, 629, 938
HpyCH4IV	ACGT	1 cut(s)	1260
HpyCH4V	TGCA	6 cut(s)	676, 797, 1039, 1091, 1144, 1459
HpyF10VI	GCNNNNNNNGC	5 cut(s)	86, 97, 178, 1150, 1159
HpyF3I	CTNAG	2 cut(s)	726, 827
HpySE526I	ACGT	1 cut(s)	1260
Hsp92I	GRCGYC	1 cut(s)	98
HspAI	GCGC	2 cut(s)	89, 364
Kzo9I	GATC	2 cut(s)	1155, 1380
LmnI	GCTCC	6 cut(s)	64, 85, 308, 490, 570, 1354
Lsp1109I	GCAGC	3 cut(s)	89, 176, 900
LweI	GCATC	5 cut(s)	760, 1078, 1261, 1288, 1307
MaeI	CTAG	2 cut(s)	117, 458
MaeII	ACGT	1 cut(s)	1260
MaeIII	GTNAC	4 cut(s)	148, 1066, 1171, 1485
MalI	GATC	2 cut(s)	1157, 1382
MbiI	CCGCTC	1 cut(s)	1153
MboI	GATC	2 cut(s)	1155, 1380
MboII	GAAGA	4 cut(s)	238, 304, 307, 571
MfeI	CAATTG	1 cut(s)	1285
MhlI	GDGCHC	2 cut(s)	20, 61
MlsI	TGGCCA	1 cut(s)	1431
MluCI	AATT	8 cut(s)	351, 447, 765, 865, 1003, 1224, 1252, 1285
MluNI	TGGCCA	1 cut(s)	1431
MlyI	GAGTC	1 cut(s)	380
MmeI	TCCRAC	1 cut(s)	973
Mox20I	TGGCCA	1 cut(s)	1431
Mph1103I	ATGCAT	1 cut(s)	1093
MscI	TGGCCA	1 cut(s)	1431
MseI	TTAA	6 cut(s)	45, 174, 260, 441, 1112, 1401
MslI	CAYNNNNRTG	1 cut(s)	1328
Msp20I	TGGCCA	1 cut(s)	1431
MspCI	CTTAAG	1 cut(s)	173
MspI	CCGG	4 cut(s)	38, 62, 942, 1502
MspR9I	CCNGG	2 cut(s)	322, 429
MunI	CAATTG	1 cut(s)	1285
MvaI	CCWGG	2 cut(s)	322, 429
MwoI	GCNNNNNNNGC	5 cut(s)	86, 97, 178, 1150, 1159
NdeI	CATATG	1 cut(s)	988
NdeII	GATC	2 cut(s)	1155, 1380
NlaIV	GGNNCC	2 cut(s)	60, 304
NmeAIII	GCCGAG	1 cut(s)	511
NmuCI	GTSAC	2 cut(s)	1171, 1485
NsiI	ATGCAT	1 cut(s)	1093
NspI	RCATGY	2 cut(s)	680, 1039
PagI	TCATGA	1 cut(s)	960
PfeI	GAWTC	5 cut(s)	536, 806, 930, 945, 983
PkrI	GCNGC	3 cut(s)	79, 166, 890
PleI	GAGTC	1 cut(s)	379
PpsI	GAGTC	1 cut(s)	379
PsiI	TTATAA	1 cut(s)	1008
Psp124BI	GAGCTC	1 cut(s)	20
Psp6I	CCWGG	2 cut(s)	320, 427
PspFI	CCCAGC	3 cut(s)	366, 1103, 1312
PspGI	CCWGG	2 cut(s)	320, 427
PspN4I	GGNNCC	2 cut(s)	60, 304
PspPI	GGNCC	2 cut(s)	420, 858
PstNI	CAGNNNCTG	1 cut(s)	706
RsaI	GTAC	2 cut(s)	416, 699
RsaNI	GTAC	2 cut(s)	415, 698
RseI	CAYNNNNRTG	1 cut(s)	1328
SacI	GAGCTC	1 cut(s)	20
SaqAI	TTAA	6 cut(s)	45, 174, 260, 441, 1112, 1401
SatI	GCNGC	3 cut(s)	78, 165, 889
Sau3AI	GATC	2 cut(s)	1155, 1380
Sau96I	GGNCC	2 cut(s)	420, 858
SchI	GAGTC	1 cut(s)	380
ScrFI	CCNGG	2 cut(s)	322, 429
SduI	GDGCHC	2 cut(s)	20, 61
SfaNI	GCATC	5 cut(s)	760, 1078, 1261, 1288, 1307
SfcI	CTRYAG	1 cut(s)	870
SinI	GGWCC	1 cut(s)	858
SmiMI	CAYNNNNRTG	1 cut(s)	1328
SmlI	CTYRAG	5 cut(s)	13, 173, 380, 602, 1343
SmoI	CTYRAG	5 cut(s)	13, 173, 380, 602, 1343
SpeI	ACTAGT	1 cut(s)	116
Sse9I	AATT	8 cut(s)	351, 447, 765, 865, 1003, 1224, 1252, 1285
SsiI	CCGC	1 cut(s)	1153
SspMI	CTAG	2 cut(s)	117, 458
SstI	GAGCTC	1 cut(s)	20
StyD4I	CCNGG	2 cut(s)	320, 427
TaaI	ACNGT	3 cut(s)	328, 629, 938
TaiI	ACGT	1 cut(s)	1263
TaqI	TCGA	5 cut(s)	288, 333, 582, 809, 981
TaqII	GACCGA	1 cut(s)	241
TasI	AATT	8 cut(s)	351, 447, 765, 865, 1003, 1224, 1252, 1285
TatI	WGTACW	1 cut(s)	697
TfiI	GAWTC	5 cut(s)	536, 806, 930, 945, 983
Tru1I	TTAA	6 cut(s)	45, 174, 260, 441, 1112, 1401
Tru9I	TTAA	6 cut(s)	45, 174, 260, 441, 1112, 1401
TscAI	CASTG	2 cut(s)	898, 943
TseFI	GTSAC	2 cut(s)	1171, 1485
TseI	GCWGC	3 cut(s)	77, 164, 888
Tsp45I	GTSAC	2 cut(s)	1171, 1485
TspDTI	ATGAA	8 cut(s)	549, 626, 878, 975, 1044, 1346, 1401, 1464
TspRI	CASTG	2 cut(s)	898, 943
Vha464I	CTTAAG	1 cut(s)	173
VpaK11BI	GGWCC	1 cut(s)	858
XagI	CCTNNNNNAGG	1 cut(s)	565
XapI	RAATTY	2 cut(s)	865, 1252
XceI	RCATGY	2 cut(s)	680, 1039
XspI	CTAG	2 cut(s)	117, 458
Zsp2I	ATGCAT	1 cut(s)	1093

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.