Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0001526

Physical Location & Seq

Genomic Coordinates Forward (+)

25411379 .. 25413959

Gene Structure 2581 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1163400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1539 bp

ATGGCCTCACTTCCTCAACCAACCCAACCACTTCCTCCGCCGTTTAACCGTCCACCGCCGCCGTCAACTGTACGGCCACAAATGACACTCAATCCCCATCCTCCTCCTTCTTCCTCTTCAACTAATACTAATGCAAACAAAACGACTTCACTGGCAGTGACTCAGAACAGTCGGAGCAAAATTCTTGAGAGTCAGAAGCTGAAAGATAAGGAGGCGAAGGAGAGGAAAGAGGAGACCAACAACAGAATAGCTTCCAGAAAAGCAATCTCGGTGATACTCCGAAGAGAAGCCACCAAAGCCCTGATTGAGAAGAAGAAGAAAGGGTCCAAGAGGCTGCTTCCCAAAACCGTGCTCGAAGCCCTCCATGAGAGAATCACTGCTCTGCGTTGGGAGTCCGCTCTCAAGGTTTTTGAACTACTACAGGAGCAGCTCTGGTACCGACCTAATGCTGCTGTATATGTTAAGCTAATCGTCATGCTTGGAAAGTGTAAGCAACCAGAAAAAGCCCATGAGCTTTTTGAAGCTATGATTGAAGAAGGCTGTGATGTCAACACTGAATCGTACACTGCTCTTGTCTCTGCCTATGGTAGGAGCGGCCTCTTTCACAAAGCATTTTCCCTCCTTGAGCGGATGAAGACTACTCCTGACTGCCAGCCTGACGTCCACACTTATTCAATCCTCATAAAATCATGCCTGCAGGTTTTTGCATATGACAAAGTGCAGGCTCTACTTTCGGATATGGAAGTTCAGGGGATAAGACCCAACACCATCACATATAATACCCTTATTGATGCCTATGGCAAATCTAAAAGATTTGCAGAGATGGAATCAACACTAGTGGAAATGCTTAGCGAACAGAATTGTGAGCCTGATGTTTGGACCATGAATTCAGTACTGAGAGCCTTTGGCAACAGCGGGCAAATAGAAACAATGGAAAAGTGTTTTGAGAGGTTTCAGAGTGCTGGGATCCAAGCCAACATCATGACTTTCAACATTCTCCTAGATTCCTATGGAAAAGCTGGGAAATATGAGAAGATGAGTGCTGTGATGGAATATATGCAGAAATACCATTATTCGTGGACGATTGTGACATATAATGTGGTGATAGACGCATTTGGGAGGGCTGGTGATTTAAAACAAATGGAGTATCTATTTAGGCTAATGCGGTCAGAGAGGATCAAACCAAGCTGTGTCACACTTTGTTCACTCGTAAGGGCATATGGTCTAGCCGGTAAACCTGAAAAAATTGAAGGTGTTTTACGTTTTGTGGAGAATTCAGATGTGATGCTCGATACAGTGTTTTTCAATTGTCTGGTTGATGCTTATGGGAGGATGGGATGCTTCGCAGAAATGAAGGGGGTGCTTGAGATAATGGAGCAAAAAGGATGTAGGCCAGACAAAGTTACATATAGAACTATGATTAAAGCTTATTCCACCAACAAGATGATTGGCCATGTGAAGGAACTCCGTGAACTTATGGAATCAGCAGAAGGGAGTCAGATGACTTGGTCCCAGAGAGAAAAACCTGACTTTCAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2 PPR_3

Protein Analysis

513

Amino Acids

58.33

Weight (kDa)

9.0

Isoelectric Point (pI)

43.05

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	148 - 194	6.1e-07	PPR repeat family
PPR_3	PF13812	172 - 231	3.7e-06	Pentatricopeptide repeat domain
PPR_1	PF12854	180 - 212	1e-05	PPR repeat
PPR_2	PF13041	184 - 231	3.6e-11	PPR repeat family
PPR	PF01535	187 - 212	2.6e-06	PPR repeat
PPR_long	PF17177	236 - 367	1.7e-07	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	243 - 304	8e-09	Pentatricopeptide repeat domain
PPR_2	PF13041	254 - 303	2.9e-10	PPR repeat family
PPR_2	PF13041	290 - 338	1e-07	PPR repeat family
PPR_3	PF13812	316 - 373	7.6e-11	Pentatricopeptide repeat domain
PPR_2	PF13041	362 - 407	3.9e-08	PPR repeat family
PPR	PF01535	363 - 392	8.6e-06	PPR repeat
TPR_24	PF23276	411 - 481	1.4e-06	Fungal tetratrico peptide repeats
PPR_2	PF13041	431 - 477	1.2e-10	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001526)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G48730
fragaria_vesca	FvH4_5g33190
malus_domestica	MD10G1163400.v1.1
prunus_persica	Prupe.8G201700_v2.0.a1 Prupe.8G201700_v2.0.a1 Prupe.8G201700_v2.0.a1
pyrus_communis	pycom10g14080
rosa_chinensis	RchiOBHm_Chr1g0373361 RchiOBHm_Chr7g0231531
rosa_laevigata	RLG00000001413 RLG00000008268
rosa_multiflora	Rmu_sc0001565.1_g000030 Rmu_sc0003505.1_g000027 Rmu_sc0005399.1_g000019 Rmu_sc0011314.1_g000012 Rmu_sc0012399.1_g000004 Rmu_sc0013558.1_g000012 Rmu_sc0028716.1_g000001
rosa_roxburghii	Rroxscaffold_1G00016380 Rroxscaffold_1G00029710 Rroxscaffold_3G00229090
rosa_rugosa	Rorug07G0267100 Rorug07G0267100
rosa_samantha	Rh1AG365000 Rh1CG099500 Rh2AG510800 Rh2BG430100 Rh2BG476000 Rh2CG061700 Rh2CG496000 Rh2DG388600 Rh2DG440000 Rh2DG484700 Rh4BG006500 Rh4DG135300 Rh5AG195600 Rh5AG195700 Rh5CG115300 Rh6AG146300 Rh7AG421500 Rh7BG396200 Rh7CG441800 Rh7DG259000 Rh7DG414200
rosa_wichuraiana	Rw0G012110 Rw6G012720 Rw6G032340 Rw6G045030 Rw7G034780 Rw7G034910 Rw7G034940

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	663
Acc36I	ACCTGC	1 cut(s)	688
Acc65I	GGTACC	1 cut(s)	435
AccB1I	GGYRCC	1 cut(s)	435
AccBSI	CCGCTC	3 cut(s)	398, 594, 628
AciI	CCGC	8 cut(s)	38, 56, 59, 396, 594, 628, 915, 1165
AclWI	GGATC	3 cut(s)	961, 974, 1184
AcoI	YGGCCR	2 cut(s)	74, 1450
AcsI	RAATTY	3 cut(s)	180, 886, 1273
AcyI	GRCGYC	1 cut(s)	660
AfaI	GTAC	4 cut(s)	72, 437, 563, 894
AfiI	CCNNNNNNNGG	2 cut(s)	588, 1459
AgsI	TTSAA	8 cut(s)	120, 413, 521, 533, 675, 991, 1250, 1306
AhlI	ACTAGT	1 cut(s)	835
AluBI	AGCT	9 cut(s)	199, 251, 430, 466, 514, 524, 1019, 1188, 1427
AluI	AGCT	9 cut(s)	199, 251, 430, 466, 514, 524, 1019, 1188, 1427
Alw21I	GWGCWC	1 cut(s)	354
Alw26I	GTCTC	2 cut(s)	227, 580
AlwI	GGATC	3 cut(s)	961, 974, 1184
AlwNI	CAGNNNCTG	1 cut(s)	199
AoxI	GGCC	5 cut(s)	3, 74, 595, 1391, 1450
ApeKI	GCWGC	3 cut(s)	334, 427, 449
ApoI	RAATTY	3 cut(s)	180, 886, 1273
Asp700I	GAANNNNTTC	1 cut(s)	250
Asp718I	GGTACC	1 cut(s)	435
AspS9I	GGNCC	3 cut(s)	324, 879, 1509
AsuHPI	GGTGA	3 cut(s)	283, 1114, 1139
AvaII	GGWCC	3 cut(s)	324, 879, 1509
BalI	TGGCCA	1 cut(s)	1452
BamHI	GGATCC	1 cut(s)	966
BanI	GGYRCC	1 cut(s)	435
BarI	GAAGNNNNNNTAC	2 cut(s)	1242, 1274
BbsI	GAAGAC	1 cut(s)	641
Bbv12I	GWGCWC	1 cut(s)	354
BbvI	GCAGC	3 cut(s)	321, 436, 439
BccI	CCATC	5 cut(s)	105, 776, 817, 1042, 1327
BceAI	ACGGC	3 cut(s)	25, 46, 89
BcoDI	GTCTC	2 cut(s)	227, 580
BcuI	ACTAGT	1 cut(s)	835
BfaI	CTAG	3 cut(s)	836, 1001, 1226
BfmI	CTRYAG	2 cut(s)	419, 695
BfuAI	ACCTGC	1 cut(s)	688
BisI	GCNGC	5 cut(s)	59, 335, 428, 450, 595
BlpI	GCTNAGC	1 cut(s)	848
BlsI	GCNGC	5 cut(s)	60, 336, 429, 451, 596
BmcAI	AGTACT	1 cut(s)	894
Bme18I	GGWCC	3 cut(s)	324, 879, 1509
BmgT120I	GGNCC	3 cut(s)	324, 879, 1509
BmiI	GGNNCC	4 cut(s)	325, 437, 968, 1511
BmsI	GCATC	4 cut(s)	781, 1275, 1309, 1328
BpiI	GAAGAC	1 cut(s)	641
Bpu1102I	GCTNAGC	1 cut(s)	848
BpuEI	CTTGAG	4 cut(s)	206, 386, 644, 1385
BsaHI	GRCGYC	1 cut(s)	660
BsaI	GGTCTC	1 cut(s)	227
BsaXI	ACNNNNNCTCC	2 cut(s)	1111, 1141
Bsc4I	CCNNNNNNNGG	2 cut(s)	588, 1459
Bse118I	RCCGGY	1 cut(s)	1229
Bse1I	ACTGG	1 cut(s)	156
BseGI	GGATG	5 cut(s)	97, 636, 1338, 1343, 1391
BseLI	CCNNNNNNNGG	2 cut(s)	588, 1459
BseMII	CTCAG	2 cut(s)	176, 887
BseNI	ACTGG	1 cut(s)	156
BseRI	GAGGAG	2 cut(s)	93, 245
BseXI	GCAGC	3 cut(s)	321, 436, 439
BseYI	CCCAGC	2 cut(s)	962, 1019
BsgI	GTGCAG	1 cut(s)	740
BshFI	GGCC	5 cut(s)	5, 76, 597, 1393, 1452
BshNI	GGYRCC	1 cut(s)	435
BsiHKAI	GWGCWC	1 cut(s)	354
BsiSI	CCGG	1 cut(s)	1230
BslFI	GGGAC	1 cut(s)	1495
BslI	CCNNNNNNNGG	2 cut(s)	588, 1459
BsmAI	GTCTC	2 cut(s)	227, 580
BsmFI	GGGAC	1 cut(s)	1495
BsnI	GGCC	5 cut(s)	5, 76, 597, 1393, 1452
Bso31I	GGTCTC	1 cut(s)	227
Bsp1286I	GDGCHC	1 cut(s)	354
Bsp143I	GATC	2 cut(s)	966, 1176
Bsp1720I	GCTNAGC	1 cut(s)	848
BspACI	CCGC	8 cut(s)	38, 56, 59, 396, 594, 628, 915, 1165
BspANI	GGCC	5 cut(s)	5, 76, 597, 1393, 1452
BspCNI	CTCAG	2 cut(s)	175, 888
BspHI	TCATGA	1 cut(s)	981
BspLI	GGNNCC	4 cut(s)	325, 437, 968, 1511
BspMAI	CTGCAG	1 cut(s)	699
BspMI	ACCTGC	1 cut(s)	688
BspPI	GGATC	3 cut(s)	961, 974, 1184
BspT107I	GGYRCC	1 cut(s)	435
BspTNI	GGTCTC	1 cut(s)	227
BsrBI	CCGCTC	3 cut(s)	398, 594, 628
BsrFI	RCCGGY	1 cut(s)	1229
BsrI	ACTGG	1 cut(s)	156
BssAI	RCCGGY	1 cut(s)	1229
BssMI	GATC	2 cut(s)	966, 1176
BssNI	GRCGYC	1 cut(s)	660
Bst4CI	ACNGT	5 cut(s)	50, 70, 170, 349, 1297
Bst6I	CTCTTC	2 cut(s)	121, 277
BstACI	GRCGYC	1 cut(s)	660
BstC8I	GCNNGC	4 cut(s)	653, 695, 723, 917
BstDEI	CTNAG	3 cut(s)	162, 848, 896
BstENI	CCTNNNNNAGG	1 cut(s)	586
BstF5I	GGATG	5 cut(s)	97, 636, 1338, 1343, 1391
BstKTI	GATC	2 cut(s)	969, 1179
BstMAI	GTCTC	2 cut(s)	227, 580
BstMBI	GATC	2 cut(s)	966, 1176
BstMWI	GCNNNNNNNGC	1 cut(s)	296
BstSFI	CTRYAG	2 cut(s)	419, 695
BstV1I	GCAGC	3 cut(s)	321, 436, 439
BstV2I	GAAGAC	1 cut(s)	641
BstX2I	RGATCY	1 cut(s)	966
BstYI	RGATCY	1 cut(s)	966
BsuRI	GGCC	5 cut(s)	5, 76, 597, 1393, 1452
BtsCI	GGATG	5 cut(s)	97, 636, 1338, 1343, 1391
BtsI	GCAGTG	3 cut(s)	162, 375, 564
BtsIMutI	CAGTG	6 cut(s)	149, 162, 375, 552, 564, 1302
BveI	ACCTGC	1 cut(s)	688
Cac8I	GCNNGC	4 cut(s)	653, 695, 723, 917
CaiI	CAGNNNCTG	1 cut(s)	199
CciI	TCATGA	1 cut(s)	981
Cfr10I	RCCGGY	1 cut(s)	1229
Cfr13I	GGNCC	3 cut(s)	324, 879, 1509
CseI	GACGC	1 cut(s)	1118
Csp6I	GTAC	4 cut(s)	71, 436, 562, 893
CspCI	CAANNNNNGTGG	2 cut(s)	819, 854
CviAII	CATG	7 cut(s)	365, 475, 509, 690, 883, 982, 1454
CviQI	GTAC	4 cut(s)	71, 436, 562, 893
DdeI	CTNAG	3 cut(s)	162, 848, 896
DpnI	GATC	2 cut(s)	968, 1178
DpnII	GATC	2 cut(s)	966, 1176
DraI	TTTAAA	1 cut(s)	1134
EaeI	YGGCCR	2 cut(s)	74, 1450
Eam1104I	CTCTTC	2 cut(s)	121, 277
EarI	CTCTTC	2 cut(s)	121, 277
EciI	GGCGGA	1 cut(s)	27
Eco31I	GGTCTC	1 cut(s)	227
Eco47I	GGWCC	3 cut(s)	324, 879, 1509
EcoNI	CCTNNNNNAGG	1 cut(s)	586
EcoRI	GAATTC	2 cut(s)	886, 1273
FaeI	CATG	7 cut(s)	368, 478, 512, 693, 886, 985, 1457
FaqI	GGGAC	1 cut(s)	1495
FatI	CATG	7 cut(s)	364, 474, 508, 689, 882, 981, 1453
FauI	CCCGC	1 cut(s)	908
FauNDI	CATATG	2 cut(s)	709, 1219
Fnu4HI	GCNGC	5 cut(s)	59, 335, 428, 450, 595
FokI	GGATG	5 cut(s)	84, 643, 1345, 1350, 1398
Fsp4HI	GCNGC	5 cut(s)	59, 335, 428, 450, 595
FspBI	CTAG	3 cut(s)	836, 1001, 1226
GluI	GCNGC	5 cut(s)	59, 335, 428, 450, 595
GsaI	CCCAGC	2 cut(s)	966, 1023
HaeIII	GGCC	5 cut(s)	5, 76, 597, 1393, 1452
HapII	CCGG	1 cut(s)	1230
HgaI	GACGC	1 cut(s)	1118
Hin1I	GRCGYC	1 cut(s)	660
Hin1II	CATG	7 cut(s)	368, 478, 512, 693, 886, 985, 1457
HincII	GTYRAC	2 cut(s)	66, 550
HindII	GTYRAC	2 cut(s)	66, 550
HindIII	AAGCTT	1 cut(s)	1425
HinfI	GANTC	9 cut(s)	160, 190, 372, 392, 557, 827, 1004, 1481, 1495
HpaII	CCGG	1 cut(s)	1230
HphI	GGTGA	3 cut(s)	283, 1114, 1139
Hpy166II	GTNNAC	9 cut(s)	53, 66, 550, 564, 664, 1080, 1205, 1235, 1472
Hpy188I	TCNGA	9 cut(s)	165, 174, 195, 281, 736, 957, 1171, 1279, 1500
Hpy188III	TCNNGA	4 cut(s)	185, 255, 644, 982
Hpy8I	GTNNAC	9 cut(s)	53, 66, 550, 564, 664, 1080, 1205, 1235, 1472
HpyAV	CCTTC	7 cut(s)	117, 211, 530, 1244, 1348, 1453, 1484
HpyCH4III	ACNGT	5 cut(s)	50, 70, 170, 349, 1297
HpyCH4IV	ACGT	2 cut(s)	660, 1261
HpyCH4V	TGCA	6 cut(s)	134, 697, 707, 721, 818, 1060
HpyF10VI	GCNNNNNNNGC	1 cut(s)	296
HpyF3I	CTNAG	3 cut(s)	162, 848, 896
HpySE526I	ACGT	2 cut(s)	660, 1261
Hsp92I	GRCGYC	1 cut(s)	660
Hsp92II	CATG	7 cut(s)	368, 478, 512, 693, 886, 985, 1457
KpnI	GGTACC	1 cut(s)	439
Kzo9I	GATC	2 cut(s)	966, 1176
LmnI	GCTCC	4 cut(s)	174, 424, 591, 1375
Lsp1109I	GCAGC	3 cut(s)	321, 436, 439
LweI	GCATC	4 cut(s)	781, 1275, 1309, 1328
MaeI	CTAG	3 cut(s)	836, 1001, 1226
MaeII	ACGT	2 cut(s)	660, 1261
MaeIII	GTNAC	4 cut(s)	157, 1087, 1192, 1402
MalI	GATC	2 cut(s)	968, 1178
MbiI	CCGCTC	3 cut(s)	398, 594, 628
MboI	GATC	2 cut(s)	966, 1176
MboII	GAAGA	9 cut(s)	102, 108, 294, 322, 325, 328, 545, 646, 1045
MfeI	CAATTG	1 cut(s)	1306
MflI	RGATCY	1 cut(s)	966
MhlI	GDGCHC	1 cut(s)	354
MlsI	TGGCCA	1 cut(s)	1452
MluCI	AATT	6 cut(s)	180, 859, 886, 1245, 1273, 1306
MluNI	TGGCCA	1 cut(s)	1452
MlyI	GAGTC	4 cut(s)	154, 199, 401, 1504
MmeI	TCCRAC	1 cut(s)	152
Mox20I	TGGCCA	1 cut(s)	1452
MroXI	GAANNNNTTC	1 cut(s)	250
MscI	TGGCCA	1 cut(s)	1452
MseI	TTAA	4 cut(s)	45, 462, 1133, 1422
Msp20I	TGGCCA	1 cut(s)	1452
MspA1I	CMGCKG	1 cut(s)	915
MspI	CCGG	1 cut(s)	1230
MunI	CAATTG	1 cut(s)	1306
MwoI	GCNNNNNNNGC	1 cut(s)	296
NdeI	CATATG	2 cut(s)	709, 1219
NdeII	GATC	2 cut(s)	966, 1176
NlaIII	CATG	7 cut(s)	368, 478, 512, 693, 886, 985, 1457
NlaIV	GGNNCC	4 cut(s)	325, 437, 968, 1511
NmuCI	GTSAC	3 cut(s)	157, 1087, 1192
PagI	TCATGA	1 cut(s)	981
PdmI	GAANNNNTTC	1 cut(s)	250
PfeI	GAWTC	5 cut(s)	372, 557, 827, 1004, 1481
PflFI	GACNNNGTC	1 cut(s)	1507
PkrI	GCNGC	5 cut(s)	60, 336, 429, 451, 596
PleI	GAGTC	4 cut(s)	154, 198, 400, 1503
PpsI	GAGTC	4 cut(s)	154, 198, 400, 1503
PspFI	CCCAGC	2 cut(s)	962, 1019
PspN4I	GGNNCC	4 cut(s)	325, 437, 968, 1511
PspPI	GGNCC	3 cut(s)	324, 879, 1509
PstI	CTGCAG	1 cut(s)	699
PstNI	CAGNNNCTG	1 cut(s)	199
PsuI	RGATCY	1 cut(s)	966
PsyI	GACNNNGTC	1 cut(s)	1507
RsaI	GTAC	4 cut(s)	72, 437, 563, 894
RsaNI	GTAC	4 cut(s)	71, 436, 562, 893
SaqAI	TTAA	4 cut(s)	45, 462, 1133, 1422
SatI	GCNGC	5 cut(s)	59, 335, 428, 450, 595
Sau3AI	GATC	2 cut(s)	966, 1176
Sau96I	GGNCC	3 cut(s)	324, 879, 1509
SbfI	CCTGCAGG	1 cut(s)	699
ScaI	AGTACT	1 cut(s)	894
SchI	GAGTC	4 cut(s)	154, 199, 401, 1504
SdaI	CCTGCAGG	1 cut(s)	699
SduI	GDGCHC	1 cut(s)	354
SfaNI	GCATC	4 cut(s)	781, 1275, 1309, 1328
SfcI	CTRYAG	2 cut(s)	419, 695
SinI	GGWCC	3 cut(s)	324, 879, 1509
SmlI	CTYRAG	4 cut(s)	185, 401, 623, 1364
SmoI	CTYRAG	4 cut(s)	185, 401, 623, 1364
SpeI	ACTAGT	1 cut(s)	835
Sse8387I	CCTGCAGG	1 cut(s)	699
Sse9I	AATT	6 cut(s)	180, 859, 886, 1245, 1273, 1306
SsiI	CCGC	8 cut(s)	38, 56, 59, 396, 594, 628, 915, 1165
SspMI	CTAG	3 cut(s)	836, 1001, 1226
TaaI	ACNGT	5 cut(s)	50, 70, 170, 349, 1297
TaiI	ACGT	2 cut(s)	663, 1264
TaqI	TCGA	2 cut(s)	354, 1290
TasI	AATT	6 cut(s)	180, 859, 886, 1245, 1273, 1306
TatI	WGTACW	1 cut(s)	892
TauI	GCSGC	2 cut(s)	61, 597
TfiI	GAWTC	5 cut(s)	372, 557, 827, 1004, 1481
Tru1I	TTAA	4 cut(s)	45, 462, 1133, 1422
Tru9I	TTAA	4 cut(s)	45, 462, 1133, 1422
TscAI	CASTG	6 cut(s)	156, 162, 382, 559, 571, 1302
TseFI	GTSAC	3 cut(s)	157, 1087, 1192
TseI	GCWGC	3 cut(s)	334, 427, 449
Tsp45I	GTSAC	3 cut(s)	157, 1087, 1192
TspDTI	ATGAA	3 cut(s)	647, 899, 1367
TspGWI	ACGGA	1 cut(s)	1457
TspRI	CASTG	6 cut(s)	156, 162, 382, 559, 571, 1302
Tth111I	GACNNNGTC	1 cut(s)	1507
VpaK11BI	GGWCC	3 cut(s)	324, 879, 1509
XagI	CCTNNNNNAGG	1 cut(s)	586
XapI	RAATTY	3 cut(s)	180, 886, 1273
XmnI	GAANNNNTTC	1 cut(s)	250
XspI	CTAG	3 cut(s)	836, 1001, 1226
ZraI	GACGTC	1 cut(s)	661
ZrmI	AGTACT	1 cut(s)	894

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.